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https://www.readbyqxmd.com/read/28158302/host-cell-interactions-of-outer-membrane-vesicle-associated-virulence-factors-of-enterohemorrhagic-escherichia-coli-o157-intracellular-delivery-trafficking-and-mechanisms-of-cell-injury
#1
Martina Bielaszewska, Christian Rüter, Andreas Bauwens, Lilo Greune, Kevin-André Jarosch, Daniel Steil, Wenlan Zhang, Xiaohua He, Roland Lloubes, Angelika Fruth, Kwang Sik Kim, M Alexander Schmidt, Ulrich Dobrindt, Alexander Mellmann, Helge Karch
Outer membrane vesicles (OMVs) are important tools in bacterial virulence but their role in the pathogenesis of infections caused by enterohemorrhagic Escherichia coli (EHEC) O157, the leading cause of life-threatening hemolytic uremic syndrome, is poorly understood. Using proteomics, electron and confocal laser scanning microscopy, immunoblotting, and bioassays, we investigated OMVs secreted by EHEC O157 clinical isolates for virulence factors cargoes, interactions with pathogenetically relevant human cells, and mechanisms of cell injury...
February 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28144586/interaction-of-bacteroides-fragilis-toxin-with-outer-membrane-vesicles-reveals-new-mechanism-of-its-secretion-and-delivery
#2
Natalya B Zakharzhevskaya, Vladimir B Tsvetkov, Anna A Vanyushkina, Anna M Varizhuk, Daria V Rakitina, Victor V Podgorsky, Innokentii E Vishnyakov, Daria D Kharlampieva, Valentin A Manuvera, Fedor V Lisitsyn, Elena A Gushina, Vassili N Lazarev, Vadim M Govorun
The only recognized virulence factor of enterotoxigenic Bacteroides fragilis (ETBF) that accompanies bloodstream infections is the zinc-dependent non-lethal metalloprotease B. fragilis toxin (BFT). The isolated toxin stimulates intestinal secretion, resulting in epithelial damage and necrosis. Numerous publications have focused on the interrelation of BFT with intestinal inflammation and colorectal neoplasia, but nothing is known about the mechanism of its secretion and delivery to host cells. However, recent studies of gram-negative bacteria have shown that outer membrane vesicles (OMVs) could be an essential mechanism for the spread of a large number of virulence factors...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28091579/impact-of-age-and-vaccination-history-on-long-term-serological-responses-after-symptomatic-b-pertussis-infection-a-high-dimensional-data-analysis
#3
Inonge van Twillert, Axel A Bonačić Marinović, Betsy Kuipers, Jacqueline A M van Gaans-van den Brink, Elisabeth A M Sanders, Cécile A C M van Els
Capturing the complexity and waning patterns of co-occurring immunoglobulin (Ig) responses after clinical B. pertussis infection may help understand how the human host gradually loses protection against whooping cough. We applied bi-exponential modelling to characterise and compare B. pertussis specific serological dynamics in a comprehensive database of IgG, IgG subclass and IgA responses to Ptx, FHA, Prn, Fim2/3 and OMV antigens of (ex-) symptomatic pertussis cases across all age groups. The decay model revealed that antigen type and age group were major factors determining differences in levels and kinetics of Ig (sub) classes...
January 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28091557/the-same-oculomotor-vermal-purkinje-cells-encode-the-different-kinematics-of-saccades-and-of-smooth-pursuit-eye-movements
#4
Zongpeng Sun, Aleksandra Smilgin, Marc Junker, Peter W Dicke, Peter Thier
Saccades and smooth pursuit eye movements (SPEM) are two types of goal-directed eye movements whose kinematics differ profoundly, a fact that may have contributed to the notion that the underlying cerebellar substrates are separated. However, it is suggested that some Purkinje cells (PCs) in the oculomotor vermis (OMV) of monkey cerebellum may be involved in both saccades and SPEM, a puzzling finding in view of the different kinematic demands of the two types of eye movements. Such 'dual' OMV PCs might be oddities with little if any functional relevance...
January 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28068479/secretable-small-rnas-via-outer-membrane-vesicles-in-periodontal-pathogens
#5
J-W Choi, S-C Kim, S-H Hong, H-J Lee
MicroRNAs (miRNAs) have been shown to be major regulators of eukaryotic gene expression. However, bacterial RNAs comparable in size to eukaryotic miRNAs (18-22 nucleotides) have received little attention. Recently, a novel class of small RNAs similar in size to miRNAs (miRNA-size, small RNAs or msRNAs) have also been found in several bacteria. Like miRNAs, msRNAs are approximately 15 to 25 nucleotides in length, and their precursors are predicted to form a hairpin loop secondary structure. Here, we identified msRNAs in the periodontal pathogens Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Treponema denticola...
January 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28054126/longitudinal-multi-modal-neuroimaging-in-opsoclonus-myoclonus-syndrome
#6
Sun-Young Oh, Rainer Boegle, Peter Zu Eulenburg, Matthias Ertl, Ji-Soo Kim, Marianne Dieterich
To investigate structural, metabolic, and functional connectivity changes in visual and oculomotor structures in a patient with paraneoplastic opsoclonus-myoclonus syndrome, serial resting-state functional and structural MRI, and FDG-PET data were collected during the acute stage and later on when the opsoclonus had resolved. In the acute stage, an FDG-PET scan demonstrated a substantially increased metabolism in structures around the deep cerebellar nuclei [e.g., fastigial nucleus (FN)] and a relatively reduced metabolism in the bilateral occipital lobes which normalized over 12 months...
January 4, 2017: Journal of Neurology
https://www.readbyqxmd.com/read/28050585/dataset-of-the-proteome-of-purified-outer-membrane-vesicles-from-the-human-pathogen-aggregatibacter-actinomycetemcomintans
#7
Thomas Kieselbach, Jan Oscarsson
The Gram-negative bacterium Aggregatibacter actinomycetemcomitans is an oral and systemic pathogen, which is linked to aggressive forms of periodontitis and can be associated with endocarditis. The outer membrane vesicles (OMVs) of this species contain effector proteins such as cytolethal distending toxin (CDT) and leukotoxin (LtxA), which they can deliver into human host cells. The OMVs can also activate innate immunity through NOD1- and NOD2-active pathogen-associated molecular patterns. This dataset provides a proteome of highly purified OMVs from A...
February 2017: Data in Brief
https://www.readbyqxmd.com/read/28036315/evaluation-of-the-immunogenic-property-of-nt-h-influenzae-protein-d-with-neisseria-meningitidis-omv-in-balb-c
#8
Ava Behrouzi, Saeid Bouzari, Seyed Davar Siadat, Mana Oloomi, Mehdi Davari, Hoora Mazaheri
INTRODUCTION: Identifying ideal non typeable Haemophilus influenzae (NTHi) vaccine candidates has not been easy due to extensive sequence and antigenic variation among gene products interacting with the immune system. Protein D (PD) is a highly conserved 42 kDa surface lipoprotein available in all H. influenzae, including NTHi. METHODOLOGY: In this study, the gene encoding PD was cloned from H. influenzae and expressed in Escheriachia coli TOPO10 cell in pBAD vector...
December 30, 2016: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/28035602/proteomic-characterization-of-the-outer-membrane-vesicle-of-the-halophilic-marine-bacterium-novosphingobium-pentaromativorans-us6-1
#9
Sung Ho Yun, Sang-Yeop Lee, Chi-Won Choi, Hayoung Lee, Hyun-Joo Ro, Sangmi Jun, Yong Min Kwon, Kae Kyoung Kwon, Sang-Jin Kim, Gun-Hwa Kim, Seung Il Kim
Novosphingobium pentaromativorans US6-1 is a Gram-negative halophilic marine bacterium able to utilize several polycyclic aromatic hydrocarbons such as phenanthrene, pyrene, and benzo[a]pyrene. In this study, using transmission electron microscopy, we confirmed that N. pentaromativorans US6-1 produces outer membrane vesicles (OMVs). N. pentaromativorans OMVs (hereafter OMVNovo) are spherical in shape, and the average diameter of OMVNovo is 25-70 nm. Proteomic analysis revealed that outer membrane proteins and periplasmic proteins of N...
January 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28031283/diversification-of-the-alpb-outer-membrane-protein-of-helicobacter-pylori-affects-biofilm-formation-and-cellular-adhesion
#10
Hideo Yonezawa, Takako Osaki, Toshiyuki Fukutomi, Tomoko Hanawa, Satoshi Kurata, Cynthia Zaman, Fuhito Hojo, Shigeru Kamiya
Helicobacter pylori is one of the most common causes of bacterial infection in humans and forms biofilms on human gastric mucosal epithelium as well as on in vitro abiotic surfaces. Bacterial biofilm is critical not only for environmental survival but also for successful infection. We previously demonstrated that strain TK1402, which was isolated from a Japanese patient with duodenal and gastric ulcers, has a high biofilm forming ability in vitro relative to other strains. In addition, we showed that outer membrane vesicles (OMV) play an important role in biofilm formation...
December 28, 2016: Journal of Bacteriology
https://www.readbyqxmd.com/read/28024958/outer-membrane-vesicles-harboring-modified-lipid-a-moiety-augment-the-efficacy-of-an-influenza-vaccine-exhibiting-reduced-endotoxicity-in-a-mouse-model
#11
Tae-Young Lee, Chang-Ung Kim, Eun-Hye Bae, Sang-Hwan Seo, Dae Gwin Jeong, Sun-Woo Yoon, Kyu-Tae Chang, Young Sang Kim, Sang-Hyun Kim, Doo-Jin Kim
Influenza is an acute respiratory disease and a major health problem worldwide. Since mucosal immunity plays a critical role in protection against influenza virus infection, mucosal immunization is considered a promising vaccination route. However, except for live-attenuated vaccines, there are no effective killed or recombinant mucosal influenza vaccines to date. Outer membrane vesicles (OMVs) are nano-sized vesicles produced by gram-negative bacteria, and contain various bacterial components capable of stimulating the immune system of the host...
December 23, 2016: Vaccine
https://www.readbyqxmd.com/read/28018313/outer-membrane-vesicles-and-soluble-factors-released-by-probiotic-escherichia-coli-nissle-1917-and-commensal-ecor63-enhance-barrier-function-by-regulating-expression-of-tight-junction-proteins-in-intestinal-epithelial-cells
#12
Carina-Shianya Alvarez, Josefa Badia, Manel Bosch, Rosa Giménez, Laura Baldomà
The gastrointestinal epithelial layer forms a physical and biochemical barrier that maintains the segregation between host and intestinal microbiota. The integrity of this barrier is critical in maintaining homeostasis in the body and its dysfunction is linked to a variety of illnesses, especially inflammatory bowel disease. Gut microbes, and particularly probiotic bacteria, modulate the barrier integrity by reducing gut permeability and reinforcing tight junctions. Probiotic Escherichia coli Nissle 1917 (EcN) is a good colonizer of the human gut with proven therapeutic efficacy in the remission of ulcerative colitis in humans...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28008331/a-meningococcal-outer-membrane-vesicle-vaccine-incorporating-genetically-attenuated-endotoxin-dissociates-inflammation-from-immunogenicity
#13
David J Dowling, Holly Sanders, Wing Ki Cheng, Sweta Joshi, Spencer Brightman, Ilana Bergelson, Carlo Pietrasanta, Simon D van Haren, Sandra van Amsterdam, Jeffrey Fernandez, Germie P J M van den Dobbelsteen, Ofer Levy
BACKGROUND: Group B Neisseria meningitidis, an endotoxin-producing Gram-negative bacterium, causes the highest incidence of group B meningococcus (MenB) disease in the first year of life. The Bexsero vaccine is indicated in Europe from 8 weeks of age. Endotoxin components of outer membrane vesicles (OMVs) or soluble lipopolysaccharide (LPS) represent a potential source of inflammation and residual reactogenicity. The purpose of this study was to compare novel candidate MenB vaccine formulations with licensed vaccines, including Bexsero, using age-specific human in vitro culture systems...
2016: Frontiers in Immunology
https://www.readbyqxmd.com/read/27996366/vitamin-e-supplementation-modulates-the-biological-effects-of-omega-3-fatty-acids-in-naturally-aged-rats
#14
Arunaksharan Narayanankutty, Anagha Kottekkat, Shaji E Mathew, Soorya P Illam, Indu M Suseela, Achuthan C Raghavamenon
Omega-3 fatty acids are well known class of nutraceuticals with established health benefits. Recently, the oxidation products of these fatty acids are gaining attention, as they are likely to disturb body redox balance. Therefore, the efficacy of omega-3 fats under conditions of diminished antioxidant status, such as ageing, is always a concern. Present study assessed the effects of omega-3 fats (DHA and EPA) together with or without vitamin-E in naturally aged rats. It was found that in omega-3 fats alone consumed rats the lipid profile was improved, while in omega-3 fat with vitamin-E consumed group (OMVE), the hepato protective and antioxidant properties were pronounced, especially the redox status of brain tissue...
December 20, 2016: Toxicology Mechanisms and Methods
https://www.readbyqxmd.com/read/27942362/immunization-with-protein-d-from-non-typeable-haemophilus-influenzae-nthi-induced-cytokine-responses-and-bioactive-antibody-production
#15
Atefeh Davoudi Vijeh Motlagh, Seyed Davar Siadat, Saeid Abedian Kenari, Mehdi Mahdavi, Ava Behrouzi, Hossein Asgarian-Omran
BACKGROUND: Outer membrane protein D (PD) is a highly conserved and stable protein in the outer membrane of both encapsulated (typeable) and non-capsulated (non-typeable) strains of Haemophilus influenzae. As an immunogen, PD is a potential candidate vaccine against non-typeable H. influenzae (NTHi) strains. OBJECTIVES: The aim of this study was to determine the cytokine pattern and the opsonic antibody response in a BALB/c mouse model versus PD from NTHi as a vaccine candidate...
October 2016: Jundishapur Journal of Microbiology
https://www.readbyqxmd.com/read/27938861/increased-production-of-outer-membrane-vesicles-by-cultured-freshwater-bacteria-in-response-to-ultraviolet-radiation
#16
Juliana P Gamalier, Thiago P Silva, Victor Zarantonello, Felipe F Dias, Rossana C N Melo
Secretion of membrane vesicles is an important biological process of both eukaryotic and prokaryotic cells. This process has been characterized in pathogenic bacteria, but is less clear in non-pathogenic bacteria from aquatic ecosystems. Here, we investigated, for the first time, the process of formation of outer membranes vesicles (OMVs), nanoscale vesicles extruded from the outer membrane (OM) of gram-negative bacteria, in cultures of freshwater bacteria after exposure or not to ultraviolet radiation (UVR) as an environmental stressor...
January 2017: Microbiological Research
https://www.readbyqxmd.com/read/27911359/directed-protein-packaging-within-outer-membrane-vesicles-from-escherichia-coli-design-production-and-purification
#17
Nathan J Alves, Kendrick B Turner, Scott A Walper
An increasing interest in applying synthetic biology techniques to program outer membrane vesicles (OMV) are leading to some very interesting and unique applications for OMV where traditional nanoparticles are proving too difficult to synthesize. To date, all Gram-negative bacteria have been shown to produce OMV demonstrating packaging of a variety of cargo that includes small molecules, peptides, proteins and genetic material. Based on their diverse cargo, OMV are implicated in many biological processes ranging from cell-cell communication to gene transfer and delivery of virulence factors depending upon which bacteria are producing the OMV...
November 16, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27858524/the-fhab-fhac-two-partner-secretion-system-is-involved-in-adhesion-of-acinetobacter-baumannii-abh12o-a2-strain
#18
A Pérez, M Merino, S Rumbo-Feal, L Álvarez-Fraga, J A Vallejo, A Beceiro, E J Ohneck, J Mateos, P Fernández-Puente, L A Actis, M Poza, G Bou
Acinetobacter baumannii is a hospital-acquired pathogen that shows an extraordinary capacity to stay in the hospital environment. Adherence of the bacteria to eukaryotic cells or to abiotic surfaces is the first step for establishing an infection. The A. baumannii strain AbH12O-A2 showed an exceptional ability to adhere to A549 epithelial cells. The AbFhaB/FhaC 2-partner secretion (TPS) system involved in adhesion was discovered after the screening of the recently determined A. baumannii AbH12O-A2 strain genome (CP009534...
November 18, 2016: Virulence
https://www.readbyqxmd.com/read/27849050/employing-escherichia-coli-derived-outer-membrane-vesicles-as-an-antigen-delivery-platform-elicits-protective-immunity-against-acinetobacter-baumannii-infection
#19
Weiwei Huang, Shijie Wang, Yufeng Yao, Ye Xia, Xu Yang, Kui Li, Pengyan Sun, Cunbao Liu, Wenjia Sun, Hongmei Bai, Xiaojie Chu, Yang Li, Yanbing Ma
Outer membrane vesicles (OMVs) have proven to be highly immunogenic and induced an immune response against bacterial infection in human clinics and animal models. We sought to investigate whether engineered OMVs can be a feasible antigen-delivery platform for efficiently inducing specific antibody responses. In this study, Omp22 (an outer membrane protein of A. baumannii) was displayed on E. coli DH5α-derived OMVs (Omp22-OMVs) using recombinant gene technology. The morphological features of Omp22-OMVs were similar to those of wild-type OMVs (wtOMVs)...
November 16, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27834207/polymorphic-regulation-of-outer-membrane-lipid-a-composition
#20
Russell E Bishop
The importance of the polymorphic-phase behavior of lipid A structural variations in determining their endotoxic activities has been recognized previously, but any potential role for lipid A polymorphism in controlling outer membrane structure and function has been largely ignored until now. In a recent article in mBio [7(5):e01532-16, https://doi.org/10.1128/mBio.01532-16], Katherine E. Bonnington and Meta J. Kuehn of Duke University's Department of Biochemistry make a compelling case for considering how the molecular shapes of the various lipid A structural subtypes found in the outer membrane contribute to the process of outer membrane vesicle (OMV) formation...
November 8, 2016: MBio
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