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https://www.readbyqxmd.com/read/27911359/directed-protein-packaging-within-outer-membrane-vesicles-from-escherichia-coli-design-production-and-purification
#1
Nathan J Alves, Kendrick B Turner, Scott A Walper
An increasing interest in applying synthetic biology techniques to program outer membrane vesicles (OMV) are leading to some very interesting and unique applications for OMV where traditional nanoparticles are proving too difficult to synthesize. To date, all Gram-negative bacteria have been shown to produce OMV demonstrating packaging of a variety of cargo that includes small molecules, peptides, proteins and genetic material. Based on their diverse cargo, OMV are implicated in many biological processes ranging from cell-cell communication to gene transfer and delivery of virulence factors depending upon which bacteria are producing the OMV...
November 16, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27858524/the-fhab-fhac-two-partner-secretion-system-is-involved-in-adhesion-of-acinetobacter-baumannii-abh12o-a2-strain
#2
A Pérez, M Merino, S Rumbo-Feal, L Álvarez-Fraga, J A Vallejo, A Beceiro, E J Ohneck, J Mateos, P Fernández-Puente, L A Actis, M Poza, G Bou
Acinetobacter baumannii is a hospital-acquired pathogen that shows an extraordinary capacity to stay in the hospital environment. Adherence of the bacteria to eukaryotic cells or to abiotic surfaces is the first step for establishing an infection. The A. baumannii strain AbH12O-A2 showed an exceptional ability to adhere to A549 epithelial cells. The AbFhaB/FhaC two-partner secretion (TPS) system involved in adhesion was discovered after the screening of the recently determined A. baumannii AbH12O-A2 strain genome (CP009534...
November 18, 2016: Virulence
https://www.readbyqxmd.com/read/27849050/employing-escherichia-coli-derived-outer-membrane-vesicles-as-an-antigen-delivery-platform-elicits-protective-immunity-against-acinetobacter-baumannii-infection
#3
Weiwei Huang, Shijie Wang, Yufeng Yao, Ye Xia, Xu Yang, Kui Li, Pengyan Sun, Cunbao Liu, Wenjia Sun, Hongmei Bai, Xiaojie Chu, Yang Li, Yanbing Ma
Outer membrane vesicles (OMVs) have proven to be highly immunogenic and induced an immune response against bacterial infection in human clinics and animal models. We sought to investigate whether engineered OMVs can be a feasible antigen-delivery platform for efficiently inducing specific antibody responses. In this study, Omp22 (an outer membrane protein of A. baumannii) was displayed on E. coli DH5α-derived OMVs (Omp22-OMVs) using recombinant gene technology. The morphological features of Omp22-OMVs were similar to those of wild-type OMVs (wtOMVs)...
November 16, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27834207/polymorphic-regulation-of-outer-membrane-lipid-a-composition
#4
Russell E Bishop
The importance of the polymorphic-phase behavior of lipid A structural variations in determining their endotoxic activities has been recognized previously, but any potential role for lipid A polymorphism in controlling outer membrane structure and function has been largely ignored until now. In a recent article in mBio [7(5):e01532-16, https://doi.org/10.1128/mBio.01532-16], Katherine E. Bonnington and Meta J. Kuehn of Duke University's Department of Biochemistry make a compelling case for considering how the molecular shapes of the various lipid A structural subtypes found in the outer membrane contribute to the process of outer membrane vesicle (OMV) formation...
November 8, 2016: MBio
https://www.readbyqxmd.com/read/27796471/deletion-of-degq-gene-enhances-outer-membrane-vesicle-production-of-shewanella-oneidensis-cells
#5
Yoshihiro Ojima, Thivagaran Mohanadas, Kosei Kitamura, Shota Nunogami, Reiki Yajima, Masahito Taya
Shewanella oneidensis is a Gram-negative facultative anaerobe that can use a wide variety of terminal electron acceptors for anaerobic respiration. In this study, S. oneidensis degQ gene, encoding a putative periplasmic serine protease, was cloned and expressed. The activity of purified DegQ was inhibited by diisopropyl fluorophosphate, a typical serine protease-specific inhibitor, indicating that DegQ is a serine protease. In-frame deletion and subsequent complementation of the degQ were carried out to examine the effect of envelope stress on the production of outer membrane vesicles (OMVs)...
October 31, 2016: Archives of Microbiology
https://www.readbyqxmd.com/read/27795394/outer-membrane-vesicle-production-facilitates-lps-remodeling-and-outer-membrane-maintenance-in-salmonella-during-environmental-transitions
#6
Katherine E Bonnington, Meta J Kuehn
: The ability of Gram-negative bacteria to carefully modulate outer membrane (OM) composition is essential to their survival. However, the asymmetric and heterogeneous structure of the Gram-negative OM poses unique challenges to the cell's successful adaption to rapid environmental transitions. Although mechanisms to recycle and degrade OM phospholipid material exist, there is no known mechanism by which to remove unfavorable lipopolysaccharide (LPS) glycoforms, except slow dilution through cell growth...
October 18, 2016: MBio
https://www.readbyqxmd.com/read/27795359/immunity-provided-by-an-outer-membrane-vesicle-cholera-vaccine-is-due-to-o-antigen-specific-antibodies-inhibiting-bacterial-motility
#7
Zhu Wang, David W Lazinski, Andrew Camilli
An outer membrane vesicle (OMV)-based cholera vaccine is highly efficacious in preventing intestinal colonization in the suckling mouse model. Immunity from OMVs comes from immunoglobulin (Ig), particularly IgG, in the milk of mucosally immunized dams. Anti-OMV IgG renders Vibrio cholerae immotile, thus they pass through the small intestine without colonizing. However, the importance of motility inhibition for protection, and the mechanism by which motility is inhibited, remain unclear. By using both in vitro and in vivo experiments, we found that IgG inhibits motility by specifically binding to the O-antigen of V...
October 24, 2016: Infection and Immunity
https://www.readbyqxmd.com/read/27773766/affinity-purification-of-bacterial-outer-membrane-vesicles-omvs-utilizing-a-his-tag-mutant
#8
Nathan J Alves, Kendrick B Turner, Kyle A DiVito, Michael A Daniele, Scott A Walper
To facilitate the rapid purification of bacterial outer membrane vesicles (OMVs), we developed two plasmid constructs that utilize a truncated, transmembrane protein to present an exterior histidine repeat sequence. We chose OmpA, a highly abundant porin protein, as the protein scaffold and utilized the lac promoter to allow for inducible control of the epitope-presenting construct. OMVs containing mutant OmpA-His6 were purified directly from Escherichia coli culture media on an immobilized metal affinity chromatography (IMAC) Ni-NTA resin...
October 20, 2016: Research in Microbiology
https://www.readbyqxmd.com/read/27771057/outer-membrane-vesicles-of-gallibacterium-anatis-induce-protective-immunity-in-egg-laying-hens
#9
Susanne E Pors, Ida J Pedersen, Ragnhild Bager Skjerning, Ida C N Thøfner, Gry Persson, Anders M Bojesen
Gallibacterium anatis causes infections in the reproductive tract of egg-laying hens and induce increased mortality and decreased egg production. New prophylactic measures are needed in order to improve animal welfare and production efficiency. Bacterial outer membrane vesicles (OMVs) have previously shown promising results in protection against infections and we hypothesized that OMVs could serve as an immunogen to protect egg-laying hens against G. anatis. To investigate the immunogenic potential of G. anatis OMVs, two in vivo studies in egg-laying hens were made...
November 15, 2016: Veterinary Microbiology
https://www.readbyqxmd.com/read/27756813/stenotrophomonas-maltophilia-outer-membrane-vesicles-elicit-a-potent-inflammatory-response-in-vitro-and-in-vivo
#10
Yoo Jeong Kim, Hyejin Jeon, Seok Hyeon Na, Hyo Il Kwon, Gati Noble Selasi, Asiimwe Nicholas, Tae In Park, Sang Hwa Lee, Je Chul Lee
Stenotrophomonas maltophilia has become one of the most prevalent opportunistic pathogens in hospitalized patients. This microorganism secretes outer membrane vesicles (OMVs), but the pathogenesis of S. maltophilia as it relates to OMVs has not been characterized. This study investigated the cytotoxic activity of S. maltophilia OMVs and their ability to induce inflammatory responses both in vitro and in vivo S. maltophilia ATCC 13637 and two clinical isolates were found to secrete spherical OMVs during in vitro culture...
October 17, 2016: Pathogens and Disease
https://www.readbyqxmd.com/read/27725813/active-shiga-like-toxin-produced-by-some-aeromonas-spp-isolated-in-mexico-city
#11
Ingrid Palma-Martínez, Andrea Guerrero-Mandujano, Manuel J Ruiz-Ruiz, Cecilia Hernández-Cortez, José Molina-López, Virgilio Bocanegra-García, Graciela Castro-Escarpulli
Shiga-like toxins (Stx) represent a group of bacterial toxins involved in human and animal diseases. Stx is produced by enterohemorrhagic Escherichia coli, Shigella dysenteriae type 1, Citrobacter freundii, and Aeromonas spp.; Stx is an important cause of bloody diarrhea and hemolytic uremic syndrome (HUS). The aim of this study was to identify the stx1/stx2 genes in clinical strains and outer membrane vesicles (OMVs) of Aeromonas spp., 66 strains were isolated from children who live in Mexico City, and Stx effects were evaluated in Vero cell cultures...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27698383/outer-membrane-vesicles-from-flagellin-deficient-salmonella-enterica-serovar-typhimurium-induce-cross-reactive-immunity-and-provide-cross-protection-against-heterologous-salmonella-challenge
#12
Qiong Liu, Qing Liu, Jie Yi, Kang Liang, Bo Hu, Xiangmin Zhang, Roy Curtiss, Qingke Kong
Outer membrane vesicles (OMVs) isolated from Salmonella Typhimurium are potentially useful for developing subunit vaccines because of high immunogenicity and protective efficacy. However, flagella might remain in OMV pellets following OMV purification, resulting in non-essential immune responses and counteraction of bacterial protective immune responses when developing a vaccine against infection of multiple serotypes Salmonella. In this study, a flagellin-deficient S. Typhimurium mutant was constructed. Lipopolysaccharide profiles, protein profiles and cryo-electron microscopy revealed that there were no significant differences between the wild-type and mutant OMVs, with the exception of a large amount of flagellin in the wild-type OMVs...
October 4, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27673272/environmentally-controlled-bacterial-vesicle-mediated-export
#13
Nichole Orench-Rivera, Meta J Kuehn
Over the past two decades, researchers studying both microbial and host cell communities have gained an appreciation for the ability of bacteria to produce, regulate, and functionally utilize outer membrane vesicles (OMVs) as a means to survive and interact with their cellular and acellular environments. Common ground has emerged, as it appears that vesicle production is an environmentally controlled and specific secretion process; however, it has been challenging to discover the principles that govern fundamentals of vesicle-mediated transport...
November 2016: Cellular Microbiology
https://www.readbyqxmd.com/read/27666736/antibiotics-and-iron-limiting-conditions-and-their-effect-on-the-production-and-composition-of-outer-membrane-vesicles-secreted-from-clinical-isolates-of-extraintestinal-pathogenic-e-coli
#14
Kin W Chan, Clifford Shone, J Richard Hesp
PURPOSE: The focus of this study was to characterize the effect of clinically relevant stress-inducing conditions on the production and composition of proinflammatory outer membrane vesicles (OMVs) produced from ST131 extraintestinal pathogenic Escherichia coli (ExPEC) clinical isolates. EXPERIMENTAL DESIGN: A label-free method (relative normalized spectral index quantification, SINQ) was used to identify changes in the respective OMV proteomes following exposure of the ExPEC strains to antibiotics and low iron...
September 26, 2016: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/27651089/immune-responses-of-a-meningococcal-a-w-outer-membrane-vesicle-omv-vaccine-with-and-without-aluminium-hydroxide-adjuvant-in-two-different-mouse-strains
#15
Gro Tunheim, Marianne Arnemo, Lisbeth M Naess, Gunnstein Norheim, Luis Garcia, Daniel Cardoso, Aleida Mandiarote, Domingo Gonzalez, Kalpana Sinnadurai, Åse-Karine Fjeldheim, Karin Bolstad, Einar Rosenqvist
Meningococci (Neisseria meningiditis) of serogroups A and W have caused large epidemics of meningitis in sub-Saharan Africa for decades, and affordable and multivalent vaccines, effective in all age groups, are needed. A bivalent serogroup A and W (A + W) meningococcal vaccine candidate consisting of deoxycholate-extracted outer membrane vesicles (OMV) from representative African disease isolates was previously found to be highly immunogenic in outbred mice when formulated with the adjuvant aluminium hydroxide (AH)...
November 2016: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/27640208/implementation-of-a-quantum-cascade-laser-based-gas-sensor-prototype-for-sub-ppmv-h2s-measurements-in-a-petrochemical-process-gas-stream
#16
Harald Moser, Walter Pölz, Johannes Paul Waclawek, Johannes Ofner, Bernhard Lendl
The implementation of a sensitive and selective as well as industrial fit gas sensor prototype based on wavelength modulation spectroscopy with second harmonic detection (2f-WMS) employing an 8-μm continuous-wave distributed feedback quantum cascade laser (CW-DFB-QCL) for monitoring hydrogen sulfide (H2S) at sub-ppm levels is reported. Regarding the applicability for analytical and industrial process purposes aimed at petrochemical environments, a synthetic methane (CH4) matrix of up to 1000 ppmv together with a varying H2S content was chosen as the model environment for the laboratory-based performance evaluation performed at TU Wien...
September 17, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27600663/a-molecularly-complete-planar-bacterial-outer-membrane-platform
#17
Chih-Yun Hsia, Linxiao Chen, Rohit R Singh, Matthew P DeLisa, Susan Daniel
The bacterial outer membrane (OM) is a barrier containing membrane proteins and liposaccharides that fulfill crucial functions for Gram-negative bacteria. With the advent of drug-resistant bacteria, it is necessary to understand the functional role of this membrane and its constituents to enable novel drug designs. Here we report a simple method to form an OM-like supported bilayer (OM-SB), which incorporates native lipids and membrane proteins of gram-negative bacteria from outer membrane vesicles (OMVs). We characterize the formation of OM-SBs using quartz crystal microbalance with dissipation (QCM-D) and fluorescence microscopy...
September 7, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27600586/outer-membrane-vesicles-in-service-as-protein-shuttles-biotic-defenders-and-immunological-doppelg%C3%A3-ngers
#18
Richard C Laughlin, Robert C Alaniz
Characterization of host microbial interactions typically occurs on the cellular or protein level. Recently, a more thorough and accurate appreciation of cellular interactions has come into better focus with improved understanding of membrane vesicles (OMV). While OMVs are documented primarily in Gram-negative bacteria, certain Gram-positive species generate these structures, despite the obvious physical limitations of the cell envelope. Here, we briefly review the current understanding of OMVs in content and function, their role in pathogenesis, and the consequences of somatic cell gene expression on these events...
September 2, 2016: Gut Microbes
https://www.readbyqxmd.com/read/27578609/outer-membrane-vesicles-derived-from-salmonella-typhimurium-mutants-with-truncated-lps-induce-cross-protective-immune-responses-against-infection-of-salmonella-enterica-serovars-in-the-mouse-model
#19
Qiong Liu, Qing Liu, Jie Yi, Kang Liang, Tian Liu, Kenneth L Roland, Yanlong Jiang, Qingke Kong
Salmonella enterica cause diarrheal and systemic diseases and are of considerable concern worldwide. Vaccines that are cross-protective against multiple serovars could provide effective control of Salmonella-mediated diseases. Bacteria-derived outer membrane vesicles (OMVs) are highly immunogenic and are capable of eliciting protective immune responses. Alterations in lipopolysaccharide (LPS) length can result in outer membrane remodeling and composition of outer membrane proteins (OMPs) changing. In this study, we investigated the impact of truncated LPS on both the production and immunogenicity of Salmonella OMVs, including the ability of OMVs to elicit cross-protection against challenge by heterologous Salmonella strains...
August 25, 2016: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/27564529/bacterial-membrane-vesicles-biogenesis-immune-regulation-and-pathogenesis
#20
Rishi D Pathirana, Maria Kaparakis-Liaskos
Outer membrane vesicles were first described approximately 50 years ago and for many years were considered to be an artifact of bacterial growth. Since that initial discovery, it has become evident that outer membrane vesicles are produced by almost all Gram-negative bacteria as part of their normal growth in addition to driving pathogenesis within the host. More recently, the identification of membrane vesicle (MV) production by some Gram-positive bacteria, parasites, fungi, mycobacteria and infected host cells has significantly broadened the field of MV research and emphasized their importance to pathogenesis...
November 2016: Cellular Microbiology
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