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https://www.readbyqxmd.com/read/29240272/amino-acid-deprivation-and-central-carbon-metabolism-regulate-the-production-of-outer-membrane-vesicles-and-tubes-by-francisella
#1
Vinaya Sampath, William D McCaig, David G Thanassi
Francisella tularensis is a highly virulent Gram-negative bacterial pathogen that causes the zoonotic disease tularemia. F. novicida, a model tularemia strain, produces spherical outer membrane vesicles (OMV), as well as novel tubular vesicles and extensions of the cell surface. These OMV and tubes (OMV/T) are produced in a regulated manner and contain known virulence factors. Mechanisms by which bacterial vesicles are produced and regulated are not well understood. We performed a genetic screen in F. novicida to decipher the molecular basis for regulated OMV/T formation, and identified both hypo- and hyper-vesiculating mutants...
December 14, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/29234030/escherichia-coli-outer-membrane-vesicles-can-contribute-to-sepsis-induced-cardiac-dysfunction
#2
Kristina Svennerholm, Kyong-Su Park, Johannes Wikström, Cecilia Lässer, Rossella Crescitelli, Ganesh V Shelke, Su Chul Jang, Shintaro Suzuki, Elga Bandeira, Charlotta S Olofsson, Jan Lötvall
Sepsis induced cardiac dysfunction (SIC) is a severe complication to sepsis which significantly worsens patient outcomes. It is known that bacteria have the capacity to release outer membrane vesicles (OMVs), which are nano-sized bilayered vesicles composed of lipids and proteins, that can induce a fatal inflammatory response. The aim of this study was to determine whether OMVs from a uropathogenic Escherichia coli strain can induce cardiac dysfunction, and to elucidate any mechanisms involved. OMVs induced irregular Ca2+ oscillations with a decreased frequency in cardiomyocytes through recordings of intracellular Ca2+ dynamics...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29232837/a-burkholderia-pseudomallei-outer-membrane-vesicle-vaccine-provides-cross-protection-against-inhalational-glanders-in-mice-and-non-human-primates
#3
Sarah M Baker, Christopher J H Davitt, Natalya Motyka, Nicole L Kikendall, Kasi Russell-Lodrigue, Chad J Roy, Lisa A Morici
Burkholderia mallei is a Gram-negative, non-motile, facultative intracellular bacillus and the causative agent of glanders, a highly contagious zoonotic disease. B. mallei is naturally resistant to multiple antibiotics and there is concern for its potential use as a bioweapon, making the development of a vaccine against B. mallei of critical importance. We have previously demonstrated that immunization with multivalent outer membrane vesicles (OMV) derived from B. pseudomallei provide significant protection against pneumonic melioidosis...
December 9, 2017: Vaccines
https://www.readbyqxmd.com/read/29208444/characterization-of-outer-membrane-vesicles-of-acetobacter-pasteurianus-nbrc3283
#4
Masahito Hashimoto, Taichi Matsumoto, Miwa Tamura-Nakano, Mami Ozono, Shuhei Hashiguchi, Yasuo Suda
Acetobacter pasteurianus is characterized as a fermenting bacterium of kurozu, which is a common traditional Japanese black vinegar. Recently, we separated acid-resistant and low Toll-like receptor 4 (TLR4)-stimulatory lipopolysaccharides (LPS) from A. pasteurianus. We also showed that their lipid A parts possessed a novel sugar backbone that is responsible for the low TLR4-stimulatory and acid-resistant properties of the LPS. Outer membrane vesicles (OMVs) are nano-sized spherical structures secreted from many gram-negative bacteria...
December 2, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/29205766/the-bile-salt-sodium-taurocholate-induces-campylobacter-jejuni-outer-membrane-vesicle-production-and-increases-omv-associated-proteolytic-activity
#5
Abdi Elmi, Amber Dorey, Eleanor Watson, Heena Jagatia, Neil F Inglis, Ozan Gundogdu, Mona Bajaj-Elliott, Brendan W Wren, David G E Smith, Nick Dorrell
Campylobacter jejuni, the leading cause of bacterial acute gastroenteritis worldwide, secretes an arsenal of virulence-associated proteins within outer membrane vesicles (OMVs). C. jejuni OMVs contain three serine proteases (HtrA, Cj0511 and Cj1365c) which cleave the intestinal epithelial cell (IEC) tight and adherens junction proteins occludin and E-cadherin, promoting enhanced C. jejuni adhesion to and invasion of IECs. C. jejuni OMVs also induce IECs innate immune responses. The bile salt sodium taurocholate (ST) is sensed as a host signal to co-ordinate the activation of virulence-associated genes in the enteric pathogen Vibrio cholerae...
December 4, 2017: Cellular Microbiology
https://www.readbyqxmd.com/read/29203497/outer-membrane-vesicles-omv-based-and-proteomics-driven-antigen-selection-identifies-novel-factors-contributing-to-bordetella-pertussis-adhesion-to-epithelial-cells
#6
Gianmarco Gasperini, Massimiliano Biagini, Vanessa Arato, Claudia Gianfaldoni, Alessandro Vadi, Nathalie Norais, Giuliano Bensi, Isabel Delany, Mariagrazia Pizza, Beatrice Aricò, Rosanna Leuzzi
Despite high vaccination coverage world-wide, whooping cough, a highly contagious disease caused by Bordetella pertussis, is recently increasing in occurrence suggesting that novel vaccine formulations targeted at the prevention of colonization and transmission should be investigated. In order to identify new candidates for inclusion in the acellular formulation, we used spontaneously released outer membrane vesicles (OMV) as a potential source of key adhesins. The enrichment of Bvg+ OMV with adhesins and the ability of anti-OMV serum to inhibit the adhesion of B...
December 4, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/29191736/immunogenicity-of-vibrio-cholerae-outer-membrane-vesicles-secreted-at-various-environmental-conditions
#7
Rezaei Adriani, Seyed Latif Mousavi Gargari, Shahram Nazarian, Samaneh Sarvary, Nafiseh Noroozi
Cholera is caused by toxigenic Vibrio cholerae. It is a significant health problem and an important cause of mortality of children in developing countries. Annually, about 5-7 million people are being infected worldwide, leading to death of 100,000 to 120,000. Immunization using the currently available cholera vaccines has been recommended by World Health Organization (WHO) in areas where cholera is endemic or at risk of outbreaks. Gram-negative bacteria secrete outer membrane vesicles (OMVs) that play important roles in virulence and host-pathogen interaction...
November 27, 2017: Vaccine
https://www.readbyqxmd.com/read/29180369/membrane-vesicles-derived-from-bordetella-bronchiseptica-active-constituent-of-a-new-vaccine-against-infections-caused-by-this-pathogen
#8
D Bottero, M E Zurita, M E Gaillard, E Bartel, C Vercellini, D Hozbor
Bordetella bronchiseptica, a Gram-negative bacterium, causes chronic respiratory-tract infections in a wide variety of mammalian hosts-humans included, albeit rarely. We recently designed B. pertussis and B. parapertussis experimental vaccines based on outer-membrane vesicles derived from each pathogen and obtained protection against the respective infections in mice. Here, we demonstrate that outer-membrane vesicles derived from virulent-phase B. bronchiseptica (OMVBbvir+) protect mice against sublethal infection from different B...
November 27, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29164053/synergistic-protective-activity-of-tumor-specific-epitopes-engineered-in-bacterial-outer-membrane-vesicles
#9
Alberto Grandi, Michele Tomasi, Ilaria Zanella, Luisa Ganfini, Elena Caproni, Laura Fantappiè, Carmela Irene, Luca Frattini, Samine J Isaac, Enrico König, Francesca Zerbini, Simona Tavarini, Chiara Sammicheli, Fabiola Giusti, Ilaria Ferlenghi, Matteo Parri, Guido Grandi
Introduction: Bacterial outer membrane vesicles (OMVs) are naturally produced by all Gram-negative bacteria and, thanks to their plasticity and unique adjuvanticity, are emerging as an attractive vaccine platform. To test the applicability of OMVs in cancer immunotherapy, we decorated them with either one or two protective epitopes present in the B16F10EGFRvIII cell line and tested the protective activity of OMV immunization in C57BL/6 mice challenged with B16F10EGFRvIII. Materials and methods: The 14 amino acid B cell epitope of human epidermal growth factor receptor variant III (EGFRvIII) and the mutation-derived CD4+ T cell neo-epitope of kif18b gene (B16-M30) were used to decorate OMVs either alone or in combination...
2017: Frontiers in Oncology
https://www.readbyqxmd.com/read/29163452/the-opportunistic-pathogen-vibrio-vulnificus-produces-outer-membrane-vesicles-in-a-spatially-distinct-manner-related-to-capsular-polysaccharide
#10
Cheri M Hampton, Ricardo C Guerrero-Ferreira, Rachel E Storms, Jeannette V Taylor, Hong Yi, Paul A Gulig, Elizabeth R Wright
Vibrio vulnificus, a bacterial species that inhabits brackish waters, is an opportunistic pathogen of humans. V. vulnificus infections can cause acute gastroenteritis, invasive septicemia, tissue necrosis, and potentially death. Virulence factors associated with V. vulnificus include the capsular polysaccharide (CPS), lipopolysaccharide, flagellum, pili, and outer membrane vesicles (OMVs). The aims of this study were to characterize the morphology of V. vulnificus cells and the formation and arrangement of OMVs using cryo-electron microscopy (cryo-EM)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29122004/identification-and-characterization-of-serovar-independent-immunogens-in-actinobacillus-pleuropneumoniae
#11
Fabio Antenucci, Cyrielle Fougeroux, Janine T Bossé, Zofia Magnowska, Camille Roesch, Paul Langford, Peter Johannes Holst, Anders Miki Bojesen
Despite numerous actions to prevent disease, Actinobacillus pleuropneumoniae (A. pleuropneumoniae) remains a major cause of porcine pleuropneumonia, resulting in economic losses to the swine industry worldwide. In this paper, we describe the utilization of a reverse vaccinology approach for the selection and in vitro testing of serovar-independent A. pleuropneumoniae immunogens. Potential immunogens were identified in the complete genomes of three A. pleuropneumoniae strains belonging to different serovars using the following parameters: predicted outer-membrane subcellular localization; ≤ 1 trans-membrane helices; presence of a signal peptide in the protein sequence; presence in all known A...
November 9, 2017: Veterinary Research
https://www.readbyqxmd.com/read/29083947/a-deeper-mining-on-the-protein-composition-of-va-mengoc-bc%C3%A2-an-omv-based-vaccine-against-n-meningitidis-serogroup-b-and-c
#12
Yordanka Masforrol, Jeovanis Gil, Darien García, Jesús Noda, Yassel Ramos, Lázaro Betancourt, Osmany Guirola, Sonia González, Boris Acevedo, Vladimir Besada, Osvaldo Reyes, Luis Javier González
The protein composition of an Outer Membrane Vesicle (OMV) preparation that constitutes the active pharmaceutical ingredient of VA-MENGOC-BC®, an effective vaccine against Neisseria meningitidis serogroups B, and C is presented. This preparation has a high lipid content and five abundant membrane proteins (FetA, PorA, PorB, RmpM, and Opc), constituting approximately 70% of the total protein mass. The protein composition was determined by combining the use of the Hexapeptide Ligand Library and an orthogonal tandem fractionation of tryptic peptides by reverse-phase chromatography at alkaline and acid pH...
November 2, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/29048985/commentary-impact-of-meningococcal-group-b-omv-vaccines-beyond-their-brief
#13
Helen Petousis-Harris
Meningococcal group B outer membrane vesicle vaccines have been used widely in Cuba, New Zealand, and Brazil. They are immunogenic and initially assessed largely by their ability to induce serum bactericidal activity. Measures of efficacy indicate good protection against homologous strains in older children and adults. Effectiveness appears broader than predicted by immunogenicity and efficacy studies. The recent discovery that meningococcal group B OMVs may protect against the related Neisseria species N.gonorrhoeae suggests more to these interesting antigen collections than meets the eye...
October 19, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/28980823/physiopathological-roles-of-spontaneously-released-outer-membrane-vesicles-of-bordetella-pertussis
#14
Gianmarco Gasperini, Vanessa Arato, Mariagrazia Pizza, Beatrice Aricò, Rosanna Leuzzi
AIM: Bordetella pertussis has been shown to release outer membrane vesicles (OMV) both in vitro and in vivo but little is known about their biological role during the initial phases of B. pertussis infection of the airways. RESULTS: We have demonstrated that OMV are released by B. pertussis in a human ciliated-airway cell model and purified vesicles can interact with host cells. Binding and uptake are strictly Bvg-regulated and OMV-associated. Pertussis toxin contributes to host-cell intoxication...
October 5, 2017: Future Microbiology
https://www.readbyqxmd.com/read/28979120/engineered-outer-membrane-vesicle-is-potent-to-elicit-hpv16e7-specific-cellular-immunity-in-a-mouse-model-of-tc-1-graft-tumor
#15
Shijie Wang, Weiwei Huang, Kui Li, Yufeng Yao, Xu Yang, Hongmei Bai, Wenjia Sun, Cunbao Liu, Yanbing Ma
PURPOSE: Currently, therapeutic tumor vaccines under development generally lack significant effects in human clinical trials. Exploring a powerful antigen delivery system is a potential approach to improve vaccine efficacy. We sought to explore engineered bacterial outer membrane vesicles (OMVs) as a new vaccine carrier for efficiently delivering tumor antigens and provoking robust antitumor immune responses. MATERIALS AND METHODS: First, the tumoral antigen human papillomavirus type 16 early protein E7 (HPV16E7) was presented on Escherichia coli-derived OMVs by genetic engineering methods, acquiring the recombinant OMV vaccine...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28974614/inflammasome-activation-by-bacterial-outer-membrane-vesicles-requires-guanylate-binding-proteins
#16
Ryan Finethy, Sarah Luoma, Nichole Orench-Rivera, Eric M Feeley, Arun K Haldar, Masahiro Yamamoto, Thirumala-Devi Kanneganti, Meta J Kuehn, Jörn Coers
The Gram-negative bacterial cell wall component lipopolysaccharide (LPS) is recognized by the noncanonical inflammasome protein caspase-11 in the cytosol of infected host cells and thereby prompts an inflammatory immune response linked to sepsis. Host guanylate binding proteins (GBPs) promote infection-induced caspase-11 activation in tissue culture models, and yet their in vivo role in LPS-mediated sepsis has remained unexplored. LPS can be released from lysed bacteria as "free" LPS aggregates or actively secreted by live bacteria as a component of outer membrane vesicles (OMVs)...
October 3, 2017: MBio
https://www.readbyqxmd.com/read/28942175/evaluation-of-immunological-responses-to-recombinant-porin-a-protein-rpoa-from-native-strains-of-neisseria-meningitidis-serogroups-a-and-b-using-omv-as-an-adjuvant-in-balb-c-mice
#17
Parviz Afrough, Saeid Bouzari, Seyed Fazlollah Mousavi, Mohammad Reza Asadi Karam, Farzam Vaziri, Abolfazl Fateh, Ava Behrouzi, Mohammadali Malekan, Seyed Davar Siadat
Neisseria meningitidis is one of the main causes of sepsis and meningitis, which are two serious life-threatening diseases in both children and adolescents. Porin A (porA) from both serogroup A and B were cloned into the pET28a plasmid and expressed in E. coli BL21 (DE3). The protein was expressed in Escherichia coli BL21 (DE3) and confirmed by SDS-PAGE and Western blot analysis. BALB/c mice were subcutaneously injected three times with 25 μg of the recombinant PorA. Specific total IgG antibodies and isotypes were evaluated using ELISA assay...
September 20, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28935107/glycoengineered-outer-membrane-vesicles-as-a-platform-for-vaccine-development
#18
Ezequiel Valguarnera, Mario F Feldman
As we enter into the postantibiotic era, vaccines to prevent bacterial infections previously treatable with antibiotics are urgently needed. Most successful antibacterial vaccines are glycoconjugates, composed of cell surface carbohydrates chemically attached to a carrier protein. Glycoconjugate vaccines provide a safe and consistent strategy against polysaccharide-encapsulated pathogens. The best examples are the conjugate vaccines against Haemophilus influenzae type b, Streptococcus pneumoniae, and Neisseria meningitidis, all based on capsular polysaccharides...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28931823/bacterial-outer-membrane-vesicles-suppress-tumor-by-interferon-%C3%AE-mediated-antitumor-response
#19
Oh Youn Kim, Hyun Taek Park, Nhung Thi Hong Dinh, Seng Jin Choi, Jaewook Lee, Ji Hyun Kim, Seung-Woo Lee, Yong Song Gho
Gram-negative bacteria actively secrete outer membrane vesicles, spherical nano-meter-sized proteolipids enriched with outer membrane proteins, to the surroundings. Outer membrane vesicles have gained wide interests as non-living complex vaccines or delivery vehicles. However, no study has used outer membrane vesicles in treating cancer thus far. Here we investigate the potential of bacterial outer membrane vesicles as therapeutic agents to treat cancer via immunotherapy. Our results show remarkable capability of bacterial outer membrane vesicles to effectively induce long-term antitumor immune responses that can fully eradicate established tumors without notable adverse effects...
September 20, 2017: Nature Communications
https://www.readbyqxmd.com/read/28890719/outer-membrane-vesicles-prime-and-activate-macrophage-inflammasomes-and-cytokine-secretion-in-vitro-and-in-vivo
#20
Jessica D Cecil, Neil M O'Brien-Simpson, Jason C Lenzo, James A Holden, William Singleton, Alexis Perez-Gonzalez, Ashley Mansell, Eric C Reynolds
Outer membrane vesicles (OMVs) are proteoliposomes blebbed from the surface of Gram-negative bacteria. Chronic periodontitis is associated with an increase in subgingival plaque of Gram-negative bacteria, Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia. In this study, we investigated the immune-modulatory effects of P. gingivalis, T. denticola, and T. forsythia OMVs on monocytes and differentiated macrophages. All of the bacterial OMVs were phagocytosed by monocytes, M(naïve) and M(IFNγ) macrophages in a dose-dependent manner...
2017: Frontiers in Immunology
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