Imdadul Haq, Jason C Ngo, Nainika Roy, Richard L Pan, Nadiya Nawsheen, Rebecca Chiu, Ya Zhang, Masashi Fujita, Rajesh K Soni, Xuebing Wu, David A Bennett, Vilas Menon, Marta Olah, Falak Sher
BACKGROUND: Microglia, the brain's resident immune cells, play vital roles in brain development, and disorders like Alzheimer's disease (AD). Human iPSC-derived microglia (iMG) provide a promising model to study these processes. However, existing iMG generation protocols face challenges, such as prolonged differentiation time, lack of detailed characterization, and limited gene function investigation via CRISPR-Cas9. METHODS: Our integrated toolkit for in-vitro microglia functional genomics optimizes iPSC differentiation into iMG through a streamlined two-step, 20-day process, producing iMG with a normal karyotype...
April 10, 2024: Stem Cell Research & Therapy