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https://www.readbyqxmd.com/read/8713133/mosaic-type-of-the-nontoxic-nonhemaggulutinin-component-gene-in-clostridium-botulinum-type-a-strain-isolated-from-infant-botulism-in-japan
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T Kubota, S Shirakawa, S Kozaki, E Isogai, H Isogai, K Kimura, N Fujii
The gene encoding the nontoxic-nonhemaggulutinin (NTNH) component was amplified by the PCR technique using two primer sets and the DNA template from Clostridium botulinum type A strain 7I03-H isolated from infant botulism in Japan. The nucleotide sequence revealed that the NTNH gene was composed of 1,193 amino acids with a molecular weight of 130868.08. Furthermore, the N-terminal half side and C-terminal half side of the NTNH component were similar to the NTNH component of type C and type A, respectively. These results indicate that the NTNH component gene codes the mosaic NTNH component composed of type A and type C...
July 25, 1996: Biochemical and Biophysical Research Communications
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