Luke M Simpson, Luke J Fulcher, Gajanan Sathe, Abigail Brewer, Jin-Feng Zhao, Daniel R Squair, Jennifer Crooks, Melanie Wightman, Nicola T Wood, Robert Gourlay, Joby Varghese, Renata F Soares, Gopal P Sapkota
Reversible protein phosphorylation, catalyzed by protein kinases and phosphatases, is a fundamental process that controls protein function and intracellular signaling. Failure of phospho-control accounts for many human diseases. While a kinase phosphorylates multiple substrates, a substrate is often phosphorylated by multiple kinases. This renders phospho-control at the substrate level challenging, as it requires inhibition of multiple kinases, which would thus affect other kinase substrates. Here, we describe the development and application of the affinity-directed phosphatase (AdPhosphatase) system for targeted dephosphorylation of specific phospho-substrates...
January 25, 2023: Cell Chemical Biology