keyword
https://read.qxmd.com/read/38151757/setdb2-interacts-with-bubr1-to-induce-accurate-chromosome-segregation-independently-of-its-histone-methyltransferase-activity
#21
JOURNAL ARTICLE
Yanhong Tu, Haomiao Zhang, Jialin Xia, Yu Zhao, Ruifang Yang, Jing Feng, Xueyun Ma, Jing Li
SETDB2 is a H3K9 histone methyltransferase required for accurate chromosome segregation. Its H3K9 histone methyltransferase activity was reported to be associated with chromosomes during metaphase. Here, we confirm that SETDB2 is required for mitosis and accurate chromosome segregation. However, these functions are independent of its histone methyltransferase activity. Further analysis showed that SETDB2 can interact with BUBR1, and is required for CDC20 binding to BUBR1 and APC/C complex and CYCLIN B1 degradation...
December 27, 2023: FEBS Open Bio
https://read.qxmd.com/read/38144520/anti-tumor-target-screening-of-sea-cucumber-saponin-frondoside-a-a-bioinformatics-and-molecular-docking-analysis
#22
JOURNAL ARTICLE
Guangchun Liu, Shenglin Zhang, Ruoyan Lin, Xudong Cao, Lihong Yuan
Cancer remains the leading cause of death worldwide. In spite of significant advances in targeted and immunotherapeutic approaches, clinical outcomes for cancer remain poor. The aim of the present study was to investigate the potential mechanisms and therapeutic targets of Frondoside A for the treatment of liver, pancreatic, and bladder cancers. The data presented in our study demonstrated that Frondoside A reduced the viability and migration of HepG2, Panc02, and UM-UC-3 cancer cell in vitro . Moreover, we utilized the GEO database to screen and identify for differentially expressed genes (DEGs) in liver, pancreatic, and bladder cancers, which resulted in the identification of 714, 357, and 101 DEGs, respectively...
2023: Frontiers in Oncology
https://read.qxmd.com/read/38133064/exploring-the-potential-of-sirna-delivery-in-acute-myeloid-leukemia-for-therapeutic-silencing
#23
JOURNAL ARTICLE
Anyeld M Ubeda Gutierrez, K C Remant Bahadur, Joseph Brandwein, Hasan Uludağ
We investigated the feasibility of using siRNA therapy for acute myeloid leukemia (AML) by developing macromolecular carriers that facilitated intracellular delivery of siRNA. The carriers were derived from low-molecular-weight (<2 kDa) polyethyleneimine (PEI) and modified with a range of aliphatic lipids. We identified linoleic acid and lauric acid-modified PEI as optimal carriers for siRNA delivery to AML cell lines KG1 and KG1a, as well as AML patient-derived mononuclear cells. As they have been proven to be potent targets in the treatment of AML, we examined the silencing of BCL2L12 and survivin and showed how it leads to the decrease in proliferation of KG1 and stem-cell-like KG1a cells...
December 18, 2023: Nanomaterials
https://read.qxmd.com/read/38090293/comparative-bioinformatics-analysis-of-transcriptomes-between-%C3%AE-aminopropionitrile-induced-aortic-dissection-murine-model-and-human-aortic-dissection
#24
JOURNAL ARTICLE
Ke Hu, Yi Guo, Yuxuan Li, Shunchang Zhou, Yiqing Li, Weici Wang
BACKGROUND: Aortic dissection (AD) poses a great threat to the life of patients; however, there is currently no documentation of a clear pathogenic mechanism of this disease. In recent years, β-aminopropionitrile (BAPN)-induced AD in rodents has been widely used in basic research, which provides a good platform for exploring the pathogenesis of AD and drug modification. This study aimed to identify molecular markers and pathways for the diagnosis and treatment of AD by comparing a murine AD model and human AD transcriptome through a bioinformatics analysis...
November 30, 2023: Journal of Thoracic Disease
https://read.qxmd.com/read/38064284/polystyrene-microplastics-mediate-cell-cycle-arrest-apoptosis-and-autophagy-in-the-g2-m-phase-through-ros-in-grass-carp-kidney-cells
#25
JOURNAL ARTICLE
Hongmin Lu, Lulu Hou, Yue Zhang, Tiantian Guo, Yu Wang, Mingwei Xing
Microplastics (MPs) have attracted widespread worldwide attention as a new pollutant. However, the role of reactive oxygen species (ROS) and cell cycle in nephrotoxicity induced by different concentrations of polystyrene microplastics (PS-MPs) is unknown. This study used grass carp kidney cells (CIK) treated with different concentrations of PS-MPs (0, 0.012, 0.0625, and 0.5 mg L-1 ) as subjects. With the increase of PS-MPs concentration, the levels of ROS and malonaldehyde increased, while the level of total antioxidant capacity, superoxide Dismutase (SOD), and glutathione (GSH) activity decreased...
December 8, 2023: Environmental Toxicology
https://read.qxmd.com/read/38051285/cathepsin-v-is-correlated-with-the-prognosis-and-tumor-microenvironment-in-liver-cancer
#26
JOURNAL ARTICLE
Junyu Liu, Wen Zhang, Zhijie Wang, Yichuan Wang, Tianxing Li, Yaping Wang, Jin Ding, Beifang Ning
Recent studies have shown that high cell cycle activity negatively correlates with antitumor immunity in certain cancer types. However, a similar correlation has not been proven in liver cancer. We downloaded transcriptomic profiles of the cancer genome atlas-liver hepatocellular carcinoma (TCGA-LIHC) and assessed the cell cycle distribution of samples using single sample gene set enrichment analysis (ssGSEA), termed the cell cycle score (CCS). We obtained cell cycle-related differentially expressed prognostic genes and identified CENPA, CDC20, and CTSV using LASSO regression...
December 5, 2023: Molecular Carcinogenesis
https://read.qxmd.com/read/38044951/insights-on-epithelial-cells-at-the-single-cell-level-in-hepatocellular-carcinoma-prognosis-and-response-to-chemotherapy
#27
JOURNAL ARTICLE
Wenqian Qi, Qian Zhang
Background: Hepatocellular carcinoma (HCC) originates from Epithelial cells, and epithelial lineage plasticity has become a promising research direction for advancing HCC treatment. This study aims to focus on Epithelial cells to provide target insights for detecting HCC prognosis and response to drug therapy. Methods: Single-cell RNA sequencing (scRNA-seq) data from GSE149614 were clustered using Seurat, and the differentiation and evolution of epithelial cells were analyzed by Monocle 2. Scissor+ and Scissor- Epithelial cells associated with the prognostic phenotypes of bulk RNA-seq of HCC were screened using the Scissor algorithm for differential analysis to screen candidate genes...
2023: Frontiers in Pharmacology
https://read.qxmd.com/read/38030698/functional-analysis-of-recurrent-cdc20-promoter-variants-in-human-melanoma
#28
JOURNAL ARTICLE
Paula M Godoy, Abimbola Oyedeji, Jacqueline L Mudd, Vasilios A Morikis, Anna P Zarov, Gregory D Longmore, Ryan C Fields, Charles K Kaufman
Small nucleotide variants in non-coding regions of the genome can alter transcriptional regulation, leading to changes in gene expression which can activate oncogenic gene regulatory networks. Melanoma is heavily burdened by non-coding variants, representing over 99% of total genetic variation, including the well-characterized TERT promoter mutation. However, the compendium of regulatory non-coding variants is likely still functionally under-characterized. We developed a pipeline to identify hotspots, i.e. recurrently mutated regions, in melanoma containing putatively functional non-coding somatic variants that are located within predicted melanoma-specific regulatory regions...
November 29, 2023: Communications Biology
https://read.qxmd.com/read/38030141/enhancing-bone-regeneration-through-cdc20-loaded-zif-8-nanoparticles-wrapped-in-erythrocyte-membranes-with-targeting-aptamer
#29
JOURNAL ARTICLE
Yangge Du, Tian Deng, Yihong Cheng, Qin Zhao, Haibin Xia, Yaoting Ji, Yufeng Zhang, Qing He
In the context of bone regeneration, nanoparticles harboring osteogenic factors have emerged as pivotal agents for modulating the differentiation fate of stem cells. However, persistent challenges surrounding biocompatibility, loading efficiency, and precise targeting ability warrant innovative solution. In this study, we introduce a novel nanoparticle platform founded upon the zeolitic imidazolate framework-8 (ZIF-8). This new design, CDC20@ZIF-8@eM-Apt, involves the envelopment of ZIF-8 within an erythrocyte membrane (eM) cloak, and coupled with a targeting aptamer...
November 29, 2023: Advanced Healthcare Materials
https://read.qxmd.com/read/37939859/quantitative-transcriptomic-and-proteomic-analysis-reveals-corosolic-acid-inhibiting-bladder-cancer-via-suppressing-cell-cycle-and-inducing-mitophagy-in-vitro-and-in-vivo
#30
JOURNAL ARTICLE
Anfang Cui, Xiangling Li, Xiaolei Ma, Zhigang Song, Xiao Wang, Chao Wang, Yong Xia
Corosolic acid (CA) is a plant-derived terpenoid compound with many health benefits. However, the anti-tumor effects of CA in bladder cancer remain unexplored. Here, we found that CA inhibited bladder tumor both in vitro and in vivo, and had no significant toxicity in mice. With the aid of transcriptomics and proteomics, we elucidated the regulatory network mechanism of CA inhibiting bladder cancer. Through cell viability detection, cell fluorescence staining and flow cytometry, we discovered that CA inhibited bladder cancer mainly through blocking cell cycle...
November 6, 2023: Toxicology and Applied Pharmacology
https://read.qxmd.com/read/37934103/tead-inhibition-overcomes-yap1-taz-driven-primary-and-acquired-resistance-to-krasg12c-inhibitors
#31
JOURNAL ARTICLE
A Cole Edwards, Clint A Stalnecker, Alexis Jean Morales, Khalilah E Taylor, Jennifer E Klomp, Jeffrey A Klomp, Andrew M Waters, Niranjan Sudhakar, Jill Hallin, Tracy T Tang, Peter Olson, Leonard Post, James G Christensen, Adrienne D Cox, Channing J Der
Primary/intrinsic and treatment-induced acquired resistance limit the initial response rate to and long-term efficacy of direct inhibitors of the KRASG12C mutant in cancer. To identify potential mechanisms of resistance, we applied a CRISPR/Cas9 loss-of-function screen and observed loss of multiple components of the Hippo tumor suppressor pathway, which acts to suppress YAP1/TAZ-regulated gene transcription. YAP1/TAZ activation impaired the anti-proliferative and pro-apoptotic effects of KRASG12C inhibitor (G12Ci) treatment in KRASG12C-mutant cancer cell lines...
November 7, 2023: Cancer Research
https://read.qxmd.com/read/37926298/therapeutic-role-of-2-stearoxyphenethyl-phosphocholine-targeting-microtubule-dynamics-and-wnt-%C3%AE-catenin-emt-signaling-in-human-colorectal-cancer-cells
#32
JOURNAL ARTICLE
Sang-Eun Park, Kyung-Sook Chung, So-Won Heo, Soo-Yeon Kim, Jeong-Hun Lee, Ahmed H E Hassan, Yong Sup Lee, Jae Yeol Lee, Kyung-Tae Lee
The inhibition of cell death, perturbation of microtubule dynamics, and acceleration of Wnt/β-catenin/epithelial-mesenchymal transition (EMT) signaling are fundamental processes in the progression and metastasis of colorectal cancer (CRC). To explore the role of 2-stearoxyphenethyl phosphocholine (stPEPC), an alkylphospholipid-based compound, in CRC, we conducted an MTT assay, cell cycle analysis, western blot analysis, immunoprecipitation, immunofluorescence staining, Annexin V/propidium iodide double staining, small interfering RNA gene silencing, a wound-healing assay, an invasion assay, and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay in the human CRC cell lines HT29 and HCT116...
November 3, 2023: Life Sciences
https://read.qxmd.com/read/37849739/novel-regulation-on-the-mitotic-checkpoint-revealed-by-knocking-out-cdc20
#33
JOURNAL ARTICLE
Ying Wang, Yuqing Zhang, Gang Zhang
No abstract text is available yet for this article.
2023: Frontiers in Cell and Developmental Biology
https://read.qxmd.com/read/37848988/identification-of-transcriptome-characteristics-of-granulosa-cells-and-the-possible-role-of-ube2c-in-the-pathogenesis-of-premature-ovarian-insufficiency
#34
JOURNAL ARTICLE
Dan Liu, Xiaohong Guan, Wenqiang Liu, Yanping Jia, Hong Zhou, Chenxiang Xi, Mei Zhao, Yuan Fang, Li Wu, Kunming Li
BACKGROUND: Premature ovarian insufficiency (POI) is an important cause of infertility characterized by the functional decline of the ovary. Granulosa cells (GCs) around oocytes are critical for folliculogenesis, and GC dysfunction is one of the important etiologies of POI. The aim of this study was to explore the potential biomarkers of POI by identifying hub genes and analyze the correlation of biomarkers with immune infiltration in POI using RNA profiling and bioinformatics analysis...
October 17, 2023: Journal of Ovarian Research
https://read.qxmd.com/read/37839768/transcriptome-analysis-of-meiotic-and-post-meiotic-spermatogenic-cells-reveals-the-potential-hub-genes-of-aging-on-the-decline-of-male-fertility
#35
JOURNAL ARTICLE
Yiqian Gui, Xixiang Ma, Mengneng Xiong, Yujiao Wen, Congcong Cao, Liang Zhang, Xiaoli Wang, Chunyan Liu, Huiping Zhang, Xunbin Huang, Chengliang Xiong, Feng Pan, Shuiqiao Yuan
Genetic and epigenetic changes in sperm caused by male aging may be essential factors affecting semen parameters, but the effects and specific molecular mechanisms of aging on male reproduction have not been fully clarified. In this study, to explore the effect of aging on male fertility and seek the potential molecular etiology, we performed high-throughput RNA-sequencing in isolated spermatogenic cells, including pachytene spermatocytes (marked by the completion of chromosome synapsis) and round spermatids (produced by the separation of sister chromatids) from the elderly and the young men...
October 13, 2023: Gene
https://read.qxmd.com/read/37805421/-prognostic-significance-and-immune-cell-infiltration-analysis-of-differentially-expressed-genes-in-malignant-pleural-mesothelioma
#36
JOURNAL ARTICLE
N Wang, Q N Zhao, Q Yuan, B L Zhu, W Wu
Objective: To explore and analyze differential expressed genes in malignant pleural mesothelioma (MPM) by bioinformatics method, and to study their prognostic value in MPM and their potential role in immunotherapy. Methods: In January 2022, the dataset GSE51024 was downloaded from the GEO database, and MPM (55 cases) and normal tissue (41 cases) samples were obtained. Using R software and HMDD and miRNet database, MPM-related differential genes were screened and co-expressed genes were identified. Co-expressed genes were enriched and functionally annotated, and protein-protein interaction (PPI) networks were constructed and key genes were identified using the STRING database and Cytoscape software...
September 20, 2023: Chinese Journal of Industrial Hygiene and Occupational Diseases
https://read.qxmd.com/read/37803076/ube2c-serves-as-a-prognosis-biomarker-of-uterine-corpus-endometrial-carcinoma-via-promoting-tumor-migration-and-invasion
#37
JOURNAL ARTICLE
Sijia Ma, Qian Chen, Xu Li, Jing Fu, Le Zhao
The biological functions of ubiquitin-conjugating enzymes E2 (UBE2) family members in uterine corpus endometrial carcinoma (UCEC) remains unclear. Our study aimed to systematically analyze the expression patterns, prognostic value, biological functions and molecular regulatory mechanisms of UBE2 family in UCEC. Among nine screened UBE2 family members associated with UCEC, UBE2C was the most significantly overexpressed gene with poor prognosis. High expression levels of UBE2C in UCEC was correlated with stages, histological subtypes, patient's menopause status and TP53 mutation...
October 6, 2023: Scientific Reports
https://read.qxmd.com/read/37777040/new-cycloartane-triterpenoids-from-dysoxylum-malabaricum-and-their-cytotoxic-evaluation
#38
JOURNAL ARTICLE
Nivedita Bhardwaj, Anamika Sharma, Nancy Tripathi, Bharat Goel, G Ravikanth, Santosh Kumar Guru, Shreyans K Jain
The cytotoxic dichloromethane-methanol bark extract of Dysoxylum malabaricum was subjected to bioassay-guided fractionation, followed by systematic dereplication to focus on identifying new compounds. From the bark of Dysoxylum malabaricum, two new cycloartane-type triterpenoids were isolated in addition to two previously known triterpenoids. The structuresandabsolute configurations of the isolated compounds were elucidated unambiguously via NMR, HRESIMS data, and electronic circular dichroism calculations...
September 28, 2023: Steroids
https://read.qxmd.com/read/37741593/transcriptome-profiling-reveals-transcriptional-regulation-of-protegrin-1-on-immune-defense-and-development-in-porcine-granulosa-cells
#39
JOURNAL ARTICLE
Yiqing Yang, Yuanyuan Zhou, Xuan Li, Yinlin He, Yinshan Bai, Bingyun Wang, Shengfeng Chen, Canying Liu
Protegrin-1 (PG1) is an antimicrobial peptide (AMP) that has garnered increasing attention due to its potent immune defense activity. Our previous studies demonstrated the ability of PG1 to enhance proliferation and inhibit apoptosis of porcine granulosa cells (GCs) under oxidative stress. GCs play a crucial role in ovary follicular development. However, the specific function and underlying mechanisms of AMP in follicular development still need further elucidation. The present study aimed to comprehensively explore the biological effects of PG1 on porcine GCs using transcriptome profiling by RNA sequencing technology...
September 21, 2023: Gene
https://read.qxmd.com/read/37741377/effects-of-the-insecticide-imidacloprid-on-the-post-embryonic-development-of-the-honey-bee-apis-mellifera-hymenoptera-apidae
#40
JOURNAL ARTICLE
Lenise Silva Carneiro, Carolina Gonçalves Santos, Matheus Tudor Cândido Santos de Resende, Débora Linhares Lino de Souza, Diego Dos Santos Souza, Amanda Martins da Cruz Souza, João Victor de Oliveira Motta, Pedro Henrique Ambrósio Nere, André Henrique de Oliveira, José Eduardo Serrão
The widespread use of pesticides in agriculture has been linked to declines in bee populations worldwide. Imidacloprid is a widely used systemic insecticide that can be found in the pollen and nectar of plants and has the potential to negatively impact the development of bee larvae. We investigated the effects of oral exposure to a realistic field concentration (20.5 ng g-1 ) of imidacloprid on the midgut and fat body of Apis mellifera worker larvae. Our results showed that larvae exposed to imidacloprid exhibited changes in the midgut epithelium, including disorganization of the brush border, nuclear chromatin condensation, cytoplasm vacuolization, and release of cell fragments indication cell death...
September 21, 2023: Science of the Total Environment
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