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Kalp Bhusan Prajapati, Rajesh Singh
In present study batch tests were performed to investigate the enhancement in methane production under bio-electrolysis anaerobic co-digestion of sewage sludge and food waste. The bio-electrolysis reactor system (B-EL) yield more methane 148.5 ml/g COD in comparison to reactor system without bio-electrolysis (B-CONT) 125.1 ml/g COD. Whereas bio-electrolysis reactor system (C-EL) Iron Scraps amended yield lesser methane (51.2 ml/g COD) in comparison to control bio-electrolysis reactor system without Iron scraps (C-CONT - 114...
May 10, 2018: Bioresource Technology
Sonia L La'ulu, Kimberly J Kalp, Joely A Straseski
BACKGROUND: Testosterone is commonly measured using immunoassays, yet concerns with the accuracy and quality of testing by these methods exist, particularly for low testosterone concentrations. Study objectives were to evaluate selective performance characteristics, including functional sensitivity (FS), of 5 automated immunoassays for total testosterone. METHODS: FS, imprecision, assay interference, limit of blank, linearity, and accuracy were assessed using the Abbott ARCHITECT i2000SR , SIEMENS ADVIA Centaur and IMMULITE 2000, Beckman Coulter DxI 800, and Roche MODULAR E170...
May 12, 2018: Clinical Biochemistry
Monika Pogorzelska-Maziarz, Ericka L Kalp
BACKGROUND: In recent years, there has been a significant shift of health care delivery to nonacute care settings. However, research on staffing and resources dedicated to infection prevention and control (IPC) in these settings is lacking. METHODS: The data for this analysis come from the 2015 APIC MegaSurvey. Descriptive statistics were computed to describe infection preventionists (IPs) employed in nonacute care settings. Bivariate analyses were conducted to examine differences in facility and demographic characteristics by type of nonacute care setting...
June 1, 2017: American Journal of Infection Control
Ericka L Kalp, James F Marx, James Davis
BACKGROUND: The Association for Professionals in Infection Control and Epidemiology (APIC) MegaSurvey, administered in 2015, was completed by approximately 4,079 APIC members. The survey sought to gain a better understanding the current state of 4 components of infection prevention practice: demographic characteristics, compensation, organizational structure, and practice and competency. METHODS: The data for this analysis come from the APIC MegaSurvey Practice and Competency domain...
June 1, 2017: American Journal of Infection Control
Sara M Reese, Ericka L Kalp
No abstract text is available yet for this article.
September 1, 2016: American Journal of Infection Control
Ericka L Kalp, Monika Pogorzelska-Maziarz
No abstract text is available yet for this article.
September 1, 2016: American Journal of Infection Control
B K Komiakov, B G Guliev, A V Zagazezhev, R V Aliev
The article presents the operation results of 380 patients (170 (44,7%) men and 210 (55,3%) women) with obstruction of pyeloureteral segment at the period from 1996 to 2014. The middle age was 43,2±7,6 years old. Primary strictures took place in 302 (79,5%) patients and recurrent strictures - in 78 (20,5%). Open and laparoscopic plastic operations were performed in 248 (65,2%), endoscopic surgery - in 112 (29,5%), organo-removal surgery - in 20 (5,3%) cases out of 380 patients with obstruction of pyeloureteral segment...
2015: Vestnik Khirurgii Imeni I. I. Grekova
Frank Lippert, Kalp Juthani
OBJECTIVES: This laboratory study aimed to (a) compare the fluoride dose-response of different caries lesions created in human and bovine enamel (HE/BE) under pH-cycling conditions and (b) investigate the suitability of Knoop and Vickers surface microhardness (K-SMH/V-SMH) in comparison to transverse microradiography (TMR) to investigate lesion de- and remineralization. MATERIALS AND METHODS: Caries lesions were formed using three different protocols (Carbopol, hydroxyethylcellulose-HEC, methylcellulose-MeC) and assigned to 24 groups using V-SMH, based on a 2 (enamel types) × 3 (lesion types) × 4 (fluoride concentrations used during pH-cycling-simulating 0/250/1100/2800 ppm F as sodium fluoride dentifrices) factorial design...
November 2015: Clinical Oral Investigations
Li Xie, Lihui Jia, Shuang Liang, David P Weliky
Membrane locations of peptides and proteins are often critical to their functions. Solid-state rotational-echo double-resonance (REDOR) nuclear magnetic resonance is applied to probe the locations of two peptides via peptide (13)CO to lipid (2)H distance measurements. The peptides are KALP, an α-helical membrane-spanning peptide, and HFP, the β-sheet N-terminal fusion peptide of the HIV gp41 fusion protein that plays an important role in HIV-host cell membrane fusion. Both peptides are shown to have at least two distinct locations within the hydrocarbon core of gel-phase membranes...
January 27, 2015: Biochemistry
Matthew Kalp, Christopher H Gottschalk
No abstract text is available yet for this article.
November 25, 2014: Neurology
Luciana Ribeiro Montenegro, Leticia F G Silveira, Cintia Tusset, Margaret de Castro, Beatriz R Versiani, Ana Claudia Latronico, Berenice Bilharinho Mendonca, Ericka B Trarbach
OBJECTIVE: To investigate the role of KAL1 abnormalities in Brazilian patients with Kallmann syndrome. DESIGN: In vitro experiments. SETTING: Academic medical center. PATIENT(S): One hundred fifteen Brazilian patients (98 men) with Kallmann syndrome. INTERVENTION(S): Peripheral blood leukocytes were used to obtain DNA. MAIN OUTCOME MEASURE(S): Direct sequencing and multiplex ligation-dependent probe amplification were used to identify KAL1 abnormalities...
September 2013: Fertility and Sterility
Brianne A Couturier, Marc Roger Couturier, Kim J Kalp, Mark A Fisher
The STAT! Campy immunochromatographic assay for Campylobacter antigen was compared to culture for 500 clinical stool specimens. Antigen was detected in six culture-negative, PCR-positive specimens. C. upsaliensis, a pathogenic species that is traditionally difficult to recover in routine stool cultures, was detected in two of these culture-negative specimens. This study provides evidence that antigen testing may cross-react with at least one additional non-jejuni and -coli Campylobacter species that may be missed by routine culture for campylobacteriosis...
June 2013: Journal of Clinical Microbiology
Li Xie, Ujjayini Ghosh, Scott D Schmick, David P Weliky
Residue-specific location of peptides in the hydrophobic core of membranes was examined using (13)C-(2)H REDOR and samples in which the lipids were selectively deuterated. The transmembrane topology of the KALP peptide was validated with this approach with substantial dephasing observed for deuteration in the bilayer center and reduced or no dephasing for deuteration closer to the headgroups. Insertion of β sheet HIV and helical and β sheet influenza virus fusion peptides into the hydrophobic core of the membrane was validated in samples with extensively deuterated lipids...
January 2013: Journal of Biomolecular NMR
Jacques P F Doux, Benjamin A Hall, J Antoinette Killian
The orientation of lipid headgroups may serve as a powerful sensor of electrostatic interactions in membranes. As shown previously by (2)H NMR measurements, the headgroup of phosphatidylcholine (PC) behaves like an electrometer and varies its orientation according to the membrane surface charge. Here, we explored the use of solid-state (14)N NMR as a relatively simple and label-free method to study the orientation of the PC headgroup in model membrane systems of varying composition. We found that (14)N NMR is sufficiently sensitive to detect small changes in headgroup orientation upon introduction of positively and negatively charged lipids and we developed an approach to directly convert the (14)N quadrupolar splittings into an average orientation of the PC polar headgroup...
September 19, 2012: Biophysical Journal
N K Singh, R H Singh
In chronic diseases, the Kalpa method of therapy prescribed in Ayurveda for revitalization and overall improvement of health is discussed in this paper. Also reports here that clinical trial of Agrogyavardhini Kalpa in chronic GIT disease confirms the efficacy of Kalps therapy in boosting the general health of the patient as reflected by its nutritional status etc. Clinical symptoms also improve and thus moving that the Arogyavardhini exerts multi dimensional influence on the disease.
July 1989: Ancient Science of Life
Karla Fejfarová, Rachid Essehli, Brahim El Bali, Michal Dušek
The title compound, NH(4)YbP(2)O(7), crystallizes in the KAlP(2)O(7) structure type and consists of distorted YbO(6) octa-hedra and bent P(2)O(7) (4-) diphosphate units forming together a three-dimensional network. There are channels in the structure running along the c axis, where the NH(4) (+) cations are located. They are connected via N-H⋯O hydrogen bonds to the terminal O atoms of the diphosphate anions.
2008: Acta Crystallographica. Section E, Structure Reports Online
Sylvain DeLisle, Brett South, Jill A Anthony, Ericka Kalp, Adi Gundlapallli, Frank C Curriero, Greg E Glass, Matthew Samore, Trish M Perl
BACKGROUND: The electronic medical record (EMR) contains a rich source of information that could be harnessed for epidemic surveillance. We asked if structured EMR data could be coupled with computerized processing of free-text clinical entries to enhance detection of acute respiratory infections (ARI). METHODOLOGY: A manual review of EMR records related to 15,377 outpatient visits uncovered 280 reference cases of ARI. We used logistic regression with backward elimination to determine which among candidate structured EMR parameters (diagnostic codes, vital signs and orders for tests, imaging and medications) contributed to the detection of those reference cases...
2010: PloS One
German Bou, Elena Santillana, Anjaneyulu Sheri, Alejandro Beceiro, Jared M Sampson, Matthew Kalp, Christopher R Bethel, Anne M Distler, Sarah M Drawz, Sundar Ram Reddy Pagadala, Focco van den Akker, Robert A Bonomo, Antonio Romero, John D Buynak
Class D β-lactamases represent a growing and diverse class of penicillin-inactivating enzymes that are usually resistant to commercial β-lactamase inhibitors. As many such enzymes are found in multi-drug resistant (MDR) Acinetobacter baumannii and Pseudomonas aeruginosa, novel β-lactamase inhibitors are urgently needed. Five unique 6-alkylidene-2'-substituted penicillanic acid sulfones (1-5) were synthesized and tested against OXA-24, a clinically important β-lactamase that inactivates carbapenems and is found in A...
September 29, 2010: Journal of the American Chemical Society
Paul R Carey, Yuanyuan Chen, Bo Gong, Matthew Kalp
Raman spectra, obtained using a Raman microscope, offer a unique and incisive approach to follow interactions and reactions inside a single crystal under soak-in or soak-out conditions. The utility of this approach derives from the finding that the Raman spectra from single macromolecular crystals, under normal (non-resonance) conditions, are extremely stable, with a low "light background," and provide ideal platforms for Raman difference spectroscopy. In turn, this allows the interrogation of sub-molecular changes in very large and complex macromolecular environments...
June 2011: Biochimica et Biophysica Acta
Matthew Kalp, John D Buynak, Paul R Carey
Mechanism-based inhibitors of class A beta-lactamases, such as sulbactam, undergo a complex series of chemical reactions in the enzyme active site. Formation of a trans-enamine acyl-enzyme via a hydrolysis-prone imine is responsible for transient inhibition of the enzyme. Although the imine to enamine tautomerization is crucial to inhibition of the enzyme, there are no experimental data to suggest how this chemical transformation is catalyzed in the active site. In this report, we show that E166 acts as a general base to promote the imine to enamine tautomerization...
November 3, 2009: Biochemistry
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