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https://www.readbyqxmd.com/read/29243152/transgene-escape-and-persistence-in-an-agroecosystem-the-case-of-glyphosate-resistant-brassica-rapa-l-in-central-argentina
#1
Claudio E Pandolfo, Alejandro Presotto, Francisco Torres Carbonell, Soledad Ureta, Mónica Poverene, Miguel Cantamutto
Brassica rapa L. is an annual Brassicaceae species cultivated for oil and food production, whose wild form is a weed of crops worldwide. In temperate regions of South America and especially in the Argentine Pampas region, this species is widely distributed. During 2014, wild B. rapa populations that escaped control with glyphosate applications by farmers were found in this area. These plants were characterized by morphology and seed acidic profile, and all the characters agreed with B. rapa description. The dose-response assays showed that the biotypes were highly resistant to glyphosate...
December 14, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29233117/elizabethkingia-miricola-as-an-opportunistic-oral-pathogen-associated-with-superinfectious-complications-in-humoral-immunodeficiency-a-case-report
#2
Przemysław Zdziarski, Mariola Paściak, Klaudia Rogala, Agnieszka Korzeniowska-Kowal, Andrzej Gamian
BACKGROUND: Elizabethkingia miricola is a rare Gram-negative bacterium found in water and clinical specimens. Typical culturing methods often misidentify Elizabethkingia spp. as Flavobacterium or Chryseobacterium. Although diagnosis is based on culturing samples taken from sterile sites, such as blood, a proper identification of this bacterium requires an expertise that goes beyond the capabilities of a typical clinical laboratory. CASE PRESENTATION: A 35-year-old woman diagnosed with common variable immunodeficiency was admitted to our center...
December 12, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29229974/ensemble-modeling-approach-targeting-heterogeneous-rna-seq-data-application-to-melanoma-pseudogenes
#3
Enrico Capobianco, Camilo Valdes, Samanta Sarti, Zhijie Jiang, Laura Poliseno, Nicolas F Tsinoremas
We studied the transcriptome landscape of skin cutaneous melanoma (SKCM) using 103 primary tumor samples from TCGA, and measured the expression levels of both protein coding genes and non-coding RNAs (ncRNAs). In particular, we emphasized pseudogenes potentially relevant to this cancer. While cataloguing the profiles based on the known biotypes, all the employed RNA-Seq methods generated just a small consensus of significant biotypes. We thus designed an approach to reconcile the profiles from all methods following a simple strategy: we selected genes that were confirmed as differentially expressed by the ensemble predictions obtained in a regression model...
December 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29222921/optimizing-rna-seq-studies-to-investigate-herbicide-resistance
#4
REVIEW
Darci A Giacomini, Todd Gaines, Roland Beffa, Patrick J Tranel
Transcriptomic profiling, specifically via RNA-seq, is becoming one of the more commonly used methods for investigating non-target site resistance (NTSR) to herbicides due to its high throughput capabilities and utility in organisms with little to no previous sequence information. A review of the weed science RNA-seq literature has revealed some basic principles behind generating quality data from these types of studies. First, studies that included more replicates per biotype and took steps to control for genetic background had significantly better control of false positives and, consequently, shorter lists of potential resistance genes to sift through...
December 9, 2017: Pest Management Science
https://www.readbyqxmd.com/read/29222809/effect-of-abutment-height-on-interproximal-implant-bone-level-in-the-early-healing-a-randomized-clinical-trial
#5
Juan Blanco, Alexandre Pico, Leticia Caneiro, Lourdes Nóvoa, Pilar Batalla, Pablo Martín-Lancharro
OBJECTIVE: The aim of this randomized clinical trial was to compare the effect on the interproximal implant bone loss (IBL) of two different heights (1 and 3 mm) of definitive abutments placed at bone level implants with a platform switched design. MATERIAL AND METHODS: Twenty-two patients received forty-four implants (6.5-10 mm length and 3.5-4 mm diameter) to replace at least two adjacent missing teeth, one bridge set to each patient-two implants per bridge...
December 8, 2017: Clinical Oral Implants Research
https://www.readbyqxmd.com/read/29220517/determining-the-effectiveness-of-three-gene-pyramids-against-aphis-glycines-hemiptera-aphididae-biotypes
#6
A J Varenhorst, S R Pritchard, M E O'Neal, E W Hodgson, A K Singh
Since the discovery of Aphis glycines Matsumura (Hemiptera: Aphididae) in the United States, the primary management tactic has been foliar insecticides. Alternative management options such as host plant resistance to A. glycines have been developed and their effectiveness proved. However, the use of host plant resistance was complicated by the discovery of multiple, virulent biotypes of A. glycines in the United States that are capable of overcoming single Rag genes, Rag1 and Rag2, as well as a two-gene pyramid of Rag1+Rag2...
December 5, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/29216684/the-impact-of-corynebacterium-glucuronolyticum-on-semen-parameters-a-prospective-pre-post-treatment-study
#7
T Meštrović, B Bedenić, J Wilson, S Ljubin-Sternak, M Sviben, M Neuberg, R Ribić, G Kozina, Z Profozić
Corynebacterium glucuronolyticum (C. glucuronolyticum) is a rare isolate that is only recently being acknowledged as a potential urogenital pathogen. The bibliographical references on this bacterial species are scarce, and its influence on all semen parameters was hitherto unknown - therefore, the aim of this study was to evaluate its effects on a range of sperm quality parameters. A prospective approach to compare semen parameters before and after treatment was used in this study. C. glucuronolyticum in semen specimens was identified using analytical profile index biotyping system (API Coryne) and additionally confirmed by matrix-assisted laser desorption/ionization time-of-flight mass-spectrometry (MALDI-TOF MS), with the determination of antimicrobial susceptibility by Kirby-Bauer method...
December 7, 2017: Andrology
https://www.readbyqxmd.com/read/29213244/virus-infected-plants-altered-the-host-selection-of-encarsia-formosa-a-parasitoid-of-whiteflies
#8
Xin Liu, Gong Chen, Youjun Zhang, Wen Xie, Qingjun Wu, Shaoli Wang
The sweetpotato whitefly Bemisia tabaci (Gennadius) is one of the most invasive pest species worldwide. Q and B biotypes are the two most devastating species within the B. tabaci complex. Bemisia tabaci can vector hundreds of plant viruses that seriously threaten crop production. Endoparasitoid, Encarsia formosa Gahan, is widely used to control whiteflies, however, little is known about the effects of virus-infected plants on E. formosa parasitism of B. tabaci. Here, we reported that tomato, which was infected with Tomato Yellow Leaf Curl Virus (TYLCV), altered the host selection of E...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29207831/comparison-of-gingival-biotype-between-different-genders-based-on-measurement-of-dentopapillary-complex
#9
Aniruddha Joshi, Girish Suragimath, Sameer Anil Zope, S R Ashwinirani, Siddhartha A Varma
Introduction: Clinical and aesthetic outcomes after periodontal or implant surgical procedures are determined by anatomical and morphological characteristics of the gingiva like width of keratinized gingiva, thickness of gingiva and alveolar bone. Therefore, the knowledge of gingival biotype plays an important role in modifying the dental therapeutic procedures for the desired outcome and predictability. Aim: The aim of the present study was to assess and compare the gingival biotype among genders by clinical, photographic and radiographic parameters...
September 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/29205196/speciation-clinical-profile-antibiotic-resistance-in-aeromonas-species-isolated-from-cholera-like-illnesses-in-a-tertiary-care-hospital-in-north-india
#10
Balvinder Mohan, Nandini Sethuraman, Ritu Verma, Neelam Taneja
BACKGROUND & OBJECTIVES: Aeromonas species have been reported to cause various illnesses in humans such as wound infections, septicaemia, peritonitis and pneumonia. Their role in causation of cholera-like illness is also being increasingly recognized. This retrospective study was done to know the presence of Aeromonas as a cause of acute diarrhoea in a tertiary care hospital and to find the common species of Aeromonas causing diarrhoea and their antibiotic susceptibility patterns. METHODS: Fifty isolates of Aeromonas were obtained over a period of 15 yr from 2000 to 2014 from patients of suspected acute gastroenteritis resembling cholera...
July 2017: Indian Journal of Medical Research
https://www.readbyqxmd.com/read/29193808/molecular-epidemiological-characterization-of-brucella-isolates-from-sheep-and-yaks-in-northwest-china
#11
X Cao, Z Li, Z Liu, B Fu, Y Liu, Y Shang, J Zhou, Z Jing
Animal brucellosis is a re-emerging disease in China with high prevalence in the northwest region. A total of 66 isolates of Brucella were recovered from sheep and yaks in the Inner Mongolia, Xinjiang, Qinghai and Gansu provinces of northwest China in 2015 and 2016. Using classical biotyping and the Brucella AMOS PCR assay, all isolates were identified as Brucella melitensis biovar 3 (n = 58), B. melitensis biovar 1 (n = 1), Brucella abortus (n = 5), or Brucella suis biovar 3 (n = 2), and B. melitensis biovar 3 was found to be mainly responsible for sheep brucellosis in northwest China...
November 29, 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29193200/rapid-identification-of-microorganisms-from-platelet-concentrates-by-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-after-short-term-incubation-on-liquid-medium
#12
Yasmine Chetouane, Gregory Dubourg, Pierre Gallian, Christophe Flaudrops, Jacques Chiaroni, Eric Chabrière, Didier Raoult, Laurence Camoin-Jau
BACKGROUND: Platelets (PLTs) are especially affected by the risk of bacterial contamination. Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) is an accurate method for the routine identification of bacterial isolates in microbiology laboratories. We directly applied the MALDI-TOF method to bacterial detection in PLTs. In this study, we evaluated the sensitivity, specificity, and speed of a direct MALDI-TOF approach compared to the conventional method BACTEC. STUDY DESIGN AND METHODS: Eight bacteria associated with PLT contamination, cited by the ISBT on transfusion-transmitted infectious diseases, were spiked into PLTs for a final concentration of approximately 100 CFU/bag (n = 5 for each strain)...
November 28, 2017: Transfusion
https://www.readbyqxmd.com/read/29191475/evaluation-of-bruker-biotyper-and-vitek-ms-for-the-identification-of-candida-tropicalis-on-different-solid-culture-media
#13
He Wang, Ying Li, Xin Fan, Tzong-Shi Chiueh, Ying-Chun Xu, Po-Ren Hsueh
BACKGROUND: The aim of this study was to investigate the performance of the Bruker Biotyper matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and Vitek MS systems for identification of genetically-confirmed blood isolates of Candida tropicalis that had been grown on several types of culture media commonly used for primary fungal isolation. METHODS: Isolates included 105 from the National China Hospital Invasive Fungal Surveillance Net program (CHIF-NET) and 120 from National Taiwan University Hospital (NTUH)...
November 11, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/29183597/resistance-to-quinclorac-caused-by-the-enhanced-ability-to-detoxify-cyanide-and-its-molecular-mechanism-in-echinochloa-crus-galli-var-zelayensis
#14
Yuan Gao, Lang Pan, Yu Sun, Teng Zhang, Liyao Dong, Jun Li
Quinclorac, an auxin-type herbicide, is widely used to control barnyardgrass and some dicotyledon weeds. Echinochloa crus-galli var. zelayensis, a variety of E. crus-galli (L.) Beauv., is widespread in China and some populations have resistance to quinclorac. E. crus-galli var. zelayensis seeds with varying sensitivity to quinclorac were used in the present study. The expression of the ADP/ATP carrier protein (ANT) gene, which plays an important role in the maintenance of cellular energy balance, dramatically rose in the S biotype after exposure to quinclorac, while no change was found in two R biotypes...
November 2017: Pesticide Biochemistry and Physiology
https://www.readbyqxmd.com/read/29183596/effect-of-allelochemical-tricin-and-its-related-benzothiazine-derivative-on-photosynthetic-performance-of-herbicide-resistant-barnyardgrass
#15
Xue-Fang Yang, Kang Lei, Chui-Hua Kong, Xiao-Hua Xu
Despite increasing knowledge of allelochemicals as leads for new herbicides, relatively little is known about the mode of action of allelochemical-based herbicides on herbicide-resistant weeds. In this study, herbicidal activities of a series of allelochemical tricin-derived compounds were evaluated. Subsequently, a benzothiazine derivative 3-(2-chloro-4-methanesulfonyl)-benzoyl-hydroxy-2-methyl-2H-1,2-benzothiazine-1,1-dioxide with 4-hydroxyphenyl-pyruvate dioxygenase (HPPD) inhibiting activity was identified as a target compound on photosynthetic performance of penoxsulam-resistant versus -susceptible barnyardgrass (Echinochloa crus-galli)...
November 2017: Pesticide Biochemistry and Physiology
https://www.readbyqxmd.com/read/29177898/adaptation-of-defensive-strategies-by-the-pea-aphid-mediates-predation-risk-from-the-predatory-lady-beetle
#16
Li-Peng Fan, Fang Ouyang, Jian-Wei Su, Feng Ge
Within a species, individual animals adopt various defensive strategies to resist natural enemies, but the defensive strategies that are adopted in response to variations in predation risk are poorly understood. Here, we assessed consecutive foraging processes on cohorts of two biotypes (green and red) of the pea aphid, Acyrthosiphon pisum, by the predatory lady beetle Propylea japonica, to investigate the adaptive mechanism underlying the defensive strategy. We observed the behavioral responses of individuals (continue feeding or escape, i...
November 25, 2017: Journal of Chemical Ecology
https://www.readbyqxmd.com/read/29159175/multilocus-sequencing-of-corynebacterium-pseudotuberculosis-biotype-ovis-strains
#17
Boglárka Sellyei, Krisztián Bányai, Dániel Bartha, István Hajtós, László Fodor, László Makrai
Thirteen Corynebacterium pseudotuberculosis biotype ovis strains isolated from clinical cases of caseous lymphadenitis in Hungary were characterised using multilocus sequencing and their phylogenetic comparison was carried out on the basis of four housekeeping genes (groEL1, infB, dnaK, and leuA). The in silico analysis of the 16 frequently studied housekeeping genes showed that C. pseudotuberculosis strains could be readily distinguished from C. diphtheriae and C. ulcerans strains; however, sequences of the same genes in the two biotypes of the C...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29153517/rapid-species-and-subspecies-specific-level-classification-and-identification-of-lactobacillus-casei-group-members-using-maldi-biotyper-combined-with-clinprotools
#18
Chien-Hsun Huang, Lina Huang
Using common taxonomic methods such as 16S rDNA sequencing and physiological and biochemical analysis to identify members of the Lactobacillus casei group (LCG) is time-consuming, expensive and inaccurate. In this study, we applied matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to rapid discriminate LCG strains by creating an analytical in-house database (IHDB) and to develop a classification model for subspecies-level differentiation based on MS biomarkers using ClinProTools bioinformatics software (Bruker Daltonics, Billerica, MA)...
November 15, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/29152799/molecular-basis-for-the-differential-expression-of-the-global-regulator-viea-in-vibrio-cholerae-biotypes-directed-by-h-ns-leuo-and-quorum-sensing
#19
Julio C Ayala, Hongxia Wang, Jorge A Benitez, Anisia J Silva
VieA is a cyclic diguanylate phosphodiesterase that modulates biofilm development and motility in Vibrio cholerae O1 of the classical biotype. vieA is part of an operon encoding the VieSAB signal transduction pathway that is nearly silent in V. cholerae of the El Tor biotype. A DNA pull-down assay for proteins interacting with the vieSAB promoter identified the LysR-type regulator LeuO. We show that in classical biotype V. cholerae, LeuO cooperates with the nucleoid-associated protein H-NS to repress vieSAB transcription...
November 20, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/29150145/neuroimaging-biomarkers-as-predictors-of-treatment-outcome-in-major-depressive-disorder
#20
REVIEW
Trehani M Fonseka, Glenda M MacQueen, Sidney H Kennedy
BACKGROUND: Current practice for selecting pharmacological and non-pharmacological antidepressant treatments has yielded low response and remission rates in Major Depressive Disorder (MDD). Neuroimaging biomarkers of brain structure and function may be useful in guiding treatment selection by predicting response vs. non-response outcomes. METHODS: In this review, we summarize data from studies examining predictors of treatment response using structural and functional neuroimaging modalities, as they pertain to pharmacotherapy, psychotherapy, and stimulation treatment strategies...
October 31, 2017: Journal of Affective Disorders
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