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https://www.readbyqxmd.com/read/28431521/a-reference-floral-transcriptome-of-sexual-and-apomictic-paspalum-notatum
#1
Juan Pablo A Ortiz, Santiago Revale, Lorena A Siena, Maricel Podio, Luciana Delgado, Juliana Stein, Olivier Leblanc, Silvina C Pessino
BACKGROUND: Paspalum notatum Flügge is a subtropical grass native to South America, which includes sexual diploid and apomictic polyploid biotypes. In the past decade, a number of apomixis-associated genes were discovered in this species through genetic mapping and differential expression surveys. However, the scarce information on Paspalum sequences available in public databanks limited annotations and functional predictions for these candidates. RESULTS: We used a long-read 454/Roche FLX+ sequencing strategy to produce robust reference transcriptome datasets from florets of sexual and apomictic Paspalum notatum genotypes and delivered a list of transcripts showing differential representation in both reproductive types...
April 21, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28431114/molecular-characterization-of-cryptococcus-neoformans-isolated-from-the-environment-in-beijing-china
#2
Hongtao Dou, Huizhu Wang, Shaowei Xie, Xinxin Chen, Zhipeng Xu, Yingchun Xu
The molecular type of environmental Cryptococcus neoformans in Beijing was not clear. Our study aims to reveal the molecular characterization of C. neoformans complex from environment in Beijing, China. A total of 435 samples of pigeon droppings from 11 different homes in Beijing were collected from August to November in 2015. Pigeon droppings were inoculated onto caffeic acid cornmeal agar (CACA) to screen C. neoformans complex. Bruker Biotyper matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was performed for species identification...
April 20, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/28431083/repellent-effects-of-different-celery-varieties-in-bemisia-tabaci-hemiptera-aleyrodidae-biotype-q
#3
Hongtao Tu, Yuchuan Qin
Y-tube olfactometer and net cages experiments were used to investigate the repellent effects of different celery varieties in biotype Q of sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), on cucumber, Cucumis sativus L. (Cucurbitaceae). Y-tube olfactometer tests showed that whiteflies have strong repellent behavior to different celery varieties. Intercropping different celery varieties with cucumbers had significant repellent effects and oviposition deterrent effects in whiteflies...
April 18, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28426703/effects-of-over-expressing-a-native-gene-encoding-5-enolpyruvylshikimate-3-phosphate-synthase-epsps-on-glyphosate-resistance-in-arabidopsis-thaliana
#4
Xiao Yang, Zachery T Beres, Lin Jin, Jason T Parrish, Wanying Zhao, David Mackey, Allison A Snow
Widespread overuse of the herbicide glyphosate, the active ingredient in RoundUp®, has led to the evolution of glyphosate-resistant weed biotypes, some of which persist by overproducing the herbicide's target enzyme, 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). EPSPS is a key enzyme in the shikimic acid pathway for biosynthesis of aromatic amino acids, lignin, and defensive compounds, but little is known about how overproducing EPSPS affects downstream metabolites, growth, or lifetime fitness in the absence of glyphosate...
2017: PloS One
https://www.readbyqxmd.com/read/28424723/evidence-mechanism-and-alternative-chemical-seedbank-level-control-of-glyphosate-resistance-of-a-rigid-ryegrass-lolium-rigidum-biotype-from-southern-spain
#5
Pablo T Fernández-Moreno, Fernando Bastida, Rafael De Prado
Rigid ryegrass (Lolium rigidum) is one of the most troublesome weeds in different crops in the Mediterranean region. A rigid ryegrass biotype from an olive grove in Jaén province (Andalusía, southern Spain), potentially resistant to glyphosate (RG), was tested for its resistance level through dose-response assays using a susceptible biotype (SG). To test the hypothesis of a non-target-site-based resistance, as point mutations are far less common mechanisms of glyphosate resistance, studies were also conducted to elucidate whether resistance was associated with biochemical, metabolism, molecular and/or physiological mechanisms...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28419491/intrinsic-neural-activity-differences-among-psychotic-illnesses
#6
Matthew E Hudgens-Haney, Lauren E Ethridge, Justin B Knight, Jennifer E McDowell, Sarah K Keedy, Godfrey D Pearlson, Carol A Tamminga, Matcheri S Keshavan, John A Sweeney, Brett A Clementz
Individuals with psychosis have been reported to show either reduced or augmented brain responses under seemingly similar conditions. It is likely that inconsistent baseline-adjustment methods are partly responsible for this discrepancy. Using steady-state stimuli during a pro/antisaccade task, this study addressed the relationship between nonspecific and stimulus-related neural activity, and how these activities are modulated as a function of cognitive demands. In 98 psychosis probands (schizophrenia, schizoaffective disorder, and bipolar disorder with psychosis), neural activity was assessed during baseline and during a 5-s period in preparation for the pro/antisaccade task...
April 17, 2017: Psychophysiology
https://www.readbyqxmd.com/read/28416313/changes-of-periodontal-parameters-after-apical-surgery-correlation-of-clinical-and-cone-beam-computed-tomographic-data
#7
Thomas von Arx, Linda Antonini, Giovanni E Salvi, Michael M Bornstein
INTRODUCTION: The purpose of this study was to evaluate the changes of the marginal periodontium 1 year after apical surgery. METHODS: Clinical and radiographic (cone-beam computed tomographic) examinations of 54 teeth treated with buccal access flaps for apical surgery were performed at baseline and after 1 year. Clinical assessment included measurements of probing pocket depth, the level of gingival margin (GM), and the width of keratinized tissue. Subsequently, the clinical attachment level (CAL) and the width of the attached gingiva were calculated...
April 14, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28408689/draft-genome-sequences-of-four-yersinia-enterocolitica-strains-isolated-from-wild-ungulate-carcasses
#8
Guerrino Macori, Angelo Romano, Daniela Adriano, Elisabetta Razzuoli, Daniela Manila Bianchi, Silvia Gallina, Alberto Bellio, Lucia Decastelli
This study describes the draft genome sequences of four Yersinia enterocolitica strains, originally isolated from ungulate carcasses. These isolates were typed biochemically and two were determined to be highly virulent (biotype 1B). The draft genome sequences had a mean size of 4.77 Mb and a mean G+C content of 47.1%.
April 13, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28407081/isolation-of-early-responsive-microrna-from-diuraphis-noxia-hemiptera-aphididae-resistant-wheat
#9
Vittorio F Nicolis, Sonia-Mari Greyling, Eduard Venter
The Russian wheat aphid (Diuraphis noxia Kurdjumov) is an economically important pest of small grains in many countries. The past decades have seen the deployment of resistance-carrying wheat (Triticum aestivum L.) cultivars to control D. noxia. However, the emergence of resistance-breaking biotypes is negating this strategy. The role that noncoding RNA (ncRNA) molecules play in the wheat-D. noxia interaction has not been studied to date. This study aimed to isolate differentially regulated microRNA from a resistant and susceptible near-isogenic wheat line after aphid infestation...
April 12, 2017: Journal of Economic Entomology
https://www.readbyqxmd.com/read/28403184/low-prevalence-of-human-enteropathogenic-yersinia-spp-in-brown-rats-rattus-norvegicus-in-flanders
#10
Lieze Oscar Rouffaer, Kristof Baert, Anne-Marie Van den Abeele, Ivo Cox, Gerty Vanantwerpen, Lieven De Zutter, Diederik Strubbe, Katleen Vranckx, Luc Lens, Freddy Haesebrouck, Michel Delmée, Frank Pasmans, An Martel
Brown rats (Rattus norvegicus) have been identified as potential carriers of Yersinia enterocolitica and Y. pseudotuberculosis, the etiological agents of yersiniosis, the third most reported bacterial zoonosis in Europe. Enteropathogenic Yersinia spp. are most often isolated from rats during yersiniosis cases in animals and humans, and from rats inhabiting farms and slaughterhouses. Information is however lacking regarding the extent to which rats act as carriers of these Yersinia spp.. In 2013, 1088 brown rats across Flanders, Belgium, were tested for the presence of Yersinia species by isolation method...
2017: PloS One
https://www.readbyqxmd.com/read/28402712/investigation-of-a-staphylococcal-food-poisoning-outbreak-from-a-chantilly-cream-dessert-in-umbria-italy
#11
Laura Ercoli, Silvia Gallina, Yacine Nia, Frédéric Auvray, Sara Primavilla, Fabrizia Guidi, Benedetta Pierucci, Catia Graziotti, Lucia Decastelli, Stefania Scuota
On August 28, 2015, a staphylococcal food poisoning outbreak occurred in Umbria, Italy, affecting 24 of the 42 customers who had dinner at a local restaurant. About 3 h after ingesting a variety of foods, the customers manifested gastrointestinal symptoms. Within 24 h of notification from the hospital emergency department, Sanitary Inspectors of the local Public Health Unit performed an epidemiological investigation. A retrospective cohort study was conducted among the customers. Food and environmental samples were collected...
April 12, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28400007/evaluation-of-virulence-genes-in-yersinia-enterocolitica-strains-using-sybr-green-real-time-pcr
#12
Maria Francesca Peruzy, Nicoletta Murru, Anna Giannina Perugini, Federico Capuano, Elisabetta Delibato, Raffaelina Mercogliano, Hannu Korkeala, Yolande Therese Rose Proroga
Yersinia enterocolitica comprises six biotypes 1A, 1B, 2, 3, 4, and 5. The virulence of the strains belonging to biotypes 1B and 2-5 depends on the presence of both chromosomal and plasmid-borne genes. Strains belonging to biotype 1A do not carry the virulence plasmid pYV. However, they carry other virulence genes, such as ystB and hreP. The aim of this study was to investigate the distribution of yadA, virF, inv, ystA, ystB, myfA, hreP and ymoA virulence genes in Y. enterocolitica strains in order to select the target genes that could be used for the development of a probe-specific real-time PCR to determine the presence of Y...
August 2017: Food Microbiology
https://www.readbyqxmd.com/read/28399056/the-nasopharyngeal-microbiota-of-children-with-respiratory-infections-in-botswana
#13
Matthew S Kelly, Michael G Surette, Marek Smieja, Jeffrey M Pernica, Laura Rossi, Kathy Luinstra, Andrew P Steenhoff, Kristen A Feemster, David M Goldfarb, Tonya Arscott-Mills, Sefelani Boiditswe, Ikanyeng Rulaganyang, Charles Muthoga, Letang Gaofiwe, Tiny Mazhani, John F Rawls, Coleen K Cunningham, Samir S Shah, Patrick C Seed
BACKGROUND: Nearly half of child pneumonia deaths occur in sub-Saharan Africa. Microbial communities in the nasopharynx are a reservoir for pneumonia pathogens and remain poorly described in African children. METHODS: Nasopharyngeal swabs were collected from children with pneumonia (N=204), children with upper respiratory infection symptoms (N=55), and healthy children (N=60) in Botswana between April 2012 and April 2014. We sequenced the V3 region of the bacterial 16S ribosomal RNA gene and used partitioning around medoids to cluster samples into microbiota biotypes...
April 10, 2017: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/28397475/evaluation-of-maldi-tof-ms-for-the-identification-of-yeast-isolates-causing-bloodstream-infection
#14
Ozge Turhan, Betil Ozhak-Baysan, Oscar Zaragoza Hernandez, Halil Er, Gozde Ongut, Dilara Ogunc, Dilek Colak, Manuel Cuenca-Estrella
BACKGROUND: Infections due to Candida species are major causes of morbidity and mortality in humans, causing a diverse spectrum of clinical disease ranging from superficial and mucosal infections to invasive disease. Several authors have demonstrated that mortality is closely linked to both timing of therapy and/or source control. The rapid identification of pathogenic species is helpful to start timely and effective antifungal therapy. The aim of this study was to assess the performance of the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) system for the correct and rapid identification of yeast isolates causing bloodstream infection...
April 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28388950/expression-profiles-of-immune-mediators-in-feline-coronavirus-infected-cells-and-clinical-samples-of-feline-coronavirus-positive-cats
#15
Nikoo Safi, Amin Haghani, Shing Wei Ng, Gayathri Thevi Selvarajah, Farina Mustaffa-Kamal, Abdul Rahman Omar
BACKGROUND: There are two biotypes of feline coronavirus (FCoV): the self-limiting feline enteric coronavirus (FECV) and the feline infectious peritonitis virus (FIPV), which causes feline infectious peritonitis (FIP), a fatal disease associated with cats living in multi-cat environments. This study provides an insight on the various immune mediators detected in FCoV-positive cats which may be responsible for the development of FIP. RESULTS: In this study, using real-time PCR and multiplex bead-based immunoassay, the expression profiles of several immune mediators were examined in Crandell-Reese feline kidney (CRFK) cells infected with the feline coronavirus (FCoV) strain FIPV 79-1146 and in samples obtained from FCoV-positive cats...
April 7, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28384252/matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-for-the-identification-of-burkholderia-pseudomallei-from-asia-and-australia-and-differentiation-between-burkholderia-species
#16
Vichaya Suttisunhakul, Apinya Pumpuang, Peeraya Ekchariyawat, Vanaporn Wuthiekanun, Mindy G Elrod, Paul Turner, Bart J Currie, Rattanaphone Phetsouvanh, David A B Dance, Direk Limmathurotsakul, Sharon J Peacock, Narisara Chantratita
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is increasingly used for rapid bacterial identification. Studies of Burkholderia pseudomallei identification have involved small isolate numbers drawn from a restricted geographic region. There is a need to expand the reference database and evaluate B. pseudomallei from a wider geographic distribution that more fully captures the extensive genetic diversity of this species. Here, we describe the evaluation of over 650 isolates...
2017: PloS One
https://www.readbyqxmd.com/read/28384245/imported-human-brucellosis-in-belgium-bio-and-molecular-typing-of-bacterial-isolates-1996-2015
#17
Delphine Hanot Mambres, Samira Boarbi, Patrick Michel, Nora Bouker, Luisa Escobar-Calle, Damien Desqueper, Tiziano Fancello, Marjan Van Esbroeck, Jacques Godfroid, David Fretin, Marcella Mori
OBJECTIVES: The aim of this study was to characterize by classical biotyping and Multi-Locus variable number tandem repeats (VNTR) Analysis (MLVA) all Brucella spp. derived from human cases in Belgium from 1996 to 2015. Final goals were to determine the species and biovar, to trace-back on genetic grounds the origin of each strain when patient history and risk factors were missing, and to survey for particular trends at the national level. METHODS: A total of 37 Brucella strains, isolated from 37 patients in Belgium, were analyzed by both classical biotyping and MLVA, and the genetic patterns compared to those of human strains isolated worldwide...
2017: PloS One
https://www.readbyqxmd.com/read/28378975/a-study-of-single-nucleotide-polymorphism-in-the-ystb-gene-of-yersinia-enterocolitica-strains-isolated-from-various-wild-animal-species
#18
Agata Bancerz-Kisiel, Anna Szczerba-Turek, Aleksandra Platt-Samoraj, Maria Michalczyk, Wojciech Szweda
INTRODUCTION AND OBJECTIVE: Y. enterocolitica is the causative agent of yersiniosis. The objective of the article was a study of single nucleotide polymorphism in the ystB gene of Y. enterocolitica strains isolated from various wild animal species. MATERIALS AND METHOD: High-resolution melting (HRM) analysis was applied to identify single nucleotide polymorphism (SNP) of ystB gene fragments of 88 Y. enterocolitica biotype 1A strains isolated from wild boar, roe deer, red deer and wild ducks...
March 1, 2017: Annals of Agricultural and Environmental Medicine: AAEM
https://www.readbyqxmd.com/read/28378054/characterization-and-genetics-of-multiple-soybean-aphid-biotype-resistance-in-five-soybean-plant-introductions
#19
Curtis B Hill, Derek Shiao, Carolyn M Fox, Glen L Hartman
Five soybean plant introductions expressed antibiosis resistance to multiple soybean aphid biotypes. Two introductions had resistance genes located in the Rag1, Rag2, and Rag3 regions; one introduction had resistance genes located in the Rag1, Rag2, and rag4 regions; one introduction had resistance genes located in the Rag1 and Rag2 regions; and one introduction had a resistance gene located in the Rag2 region. Soybean aphid (Aphis glycines Matsumura) is the most important soybean [Glycine max (L.) Merr.] insect pest in the USA...
April 4, 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/28374172/evaluation-of-potential-probiotics-isolated-from-human-milk-and-colostrum
#20
Quésia S Damaceno, Jaqueline P Souza, Jacques R Nicoli, Raquel L Paula, Gabriela B Assis, Henrique C Figueiredo, Vasco Azevedo, Flaviano S Martins
Several studies have demonstrated a diversity of bacterial species in human milk, even in aseptically collected samples. The present study evaluated potential probiotic bacteria isolated from human milk and associated maternal variables. Milk samples were collected from 47 healthy women and cultured on selective and universal agar media under aerobic and anaerobic conditions. Bacterial isolates were counted and identified by Biotyper Matrix-Assisted Laser Desorption Ionization-Time of Flight mass spectrometry and then tested for probiotic properties...
April 3, 2017: Probiotics and Antimicrobial Proteins
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