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Reticulocyte count

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https://www.readbyqxmd.com/read/27897313/the-impact-of-fist-clenching-and-its-maintenance-during-venipuncture-on-routine-hematology-testing
#1
Gabriel Lima-Oliveira, Gian Cesare Guidi, Gian Luca Salvagno, Giuseppe Lippi
BACKGROUND: Prevention of a disturbance of the blood vessel allows phlebotomists to collect a blood specimen by venipuncture that will truly mirror the patient condition. This study was aimed to evaluate the impact of repeated fist clenching and maintenance of the fist during blood collection by venipuncture for routine hematology testing. METHODS: Blood were collected from 16 healthy volunteers with two separate sequential procedures, entailing standard venipuncture with hand opened throughout blood collection, or clenching the fist six times before venipuncture and maintaining the fist until completion of blood collection...
November 29, 2016: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/27869039/the-role-of-rs1984112_g-at-cd36-gene-in-increasing-reticulocyte-level-among-sickle-cell-disease-patients
#2
Miniar Kalai, Marwa Dridi, Leila Chaouch, Imen Moumni, Houyem Ouragini, Imen Darragi, Imen Boudrigua, Dorra Chaouachi, Fethi Mellouli, Mohamed Bejaoui, Salem Abbes
AIMS AND BACKGROUND: Mediators of adhesion become a potential new target for pharmacological therapy to struggle the complications of sickle cell disease (SCD). Several mechanisms for increased adherence have been postulated and the well-studied are CD36 and VLA4 which encoded by ITGA4. Herein, we sought to determine whether one polymorphism of CD36 namely: rs1984112 and three exons of ITGA4 (4, 5, and 6) are implicated in hemolytic status and clinical events among SCD Tunisian patients...
November 20, 2016: Hematology (Amsterdam, Netherlands)
https://www.readbyqxmd.com/read/27866575/anemia-for-the-primary-care-physician
#3
REVIEW
Darryl J Powell, Maureen Okam Achebe
Anemia denotes a reduced red blood cell (RBC) mass from any cause. The causes of anemia are numerous and due to decreased (or abnormal) erythropoesis, shortened RBC life span, or blood loss. The most common etiology of anemia is iron deficiency. A judicious work up of anemia includes evaluating the reticulocyte count and peripheral smear. The severity of illness of a patient with anemia is determined by the degree of anemia and the seriousness of the underlying disorder. Management of patients with hereditary and hemolytic anemias should involve a hematologist...
December 2016: Primary Care
https://www.readbyqxmd.com/read/27863758/determination-of-reference-ranges-for-immature-platelet-and-reticulocyte-fractions-and-reticulocyte-hemoglobin-equivalent
#4
Iuri Vicente Camargo Morkis, Mariela Granero Farias, Luciana Scotti
INTRODUCTION: The immature platelet and immature reticulocyte fractions represent the ratios of platelets and reticulocytes recently released into the circulation and thus with higher RNA content. They are considered early indicators of bone marrow recovery. OBJECTIVE: The aim of this study was to determine the reference ranges for the immature platelet and reticulocyte fractions of hematologically normal individuals in a university hospital. METHODS: Venous blood samples collected in ethylenediaminetetraacetic acid K3 were analyzed using a Sysmex XE-5000™ analyzer...
October 2016: Revista Brasileira de Hematologia e Hemoterapia
https://www.readbyqxmd.com/read/27857529/severe-hemolytic-disease-of-the-premature-newborn-due-to-rh1-incompatibility-a-case-report
#5
Jean Uwingabiye, Hafid Zahid, Fayçal Labrini, Abdelhak El Khazraji, Anass Yahyaoui, Rachid Hadef, Nezha Messaoudi
We report a case of dramatic outcome of severe hemolytic disease in a newborn due to RH1 incompatibility. A newborn with A RH1 blood group was admitted in the Mohammed V Military Teaching Hospital for the problem of hydrops fetalis associated with RH1 incompatibility. The blood group of his mother, aged 31, was AB RH1-negative and that of his 37 year old father was A RH1. The mother had a history of 4 term deliveries, 3 abortions, and 1 living child. There was no prevention by anti-D immunoglobulin postpartum...
2016: Clujul Medical (1957)
https://www.readbyqxmd.com/read/27843377/pain-frequency-severity-and-qt-dispersion-in-adult-patients-with-sickle-cell-anemia-correlation-with-inflammatory-markers
#6
Taysir S Garadah, Ahmed A Jaradat, Mohammed E AlAlawi, Adla B Hassan, Reginald P Sequeira
BACKGROUND: Inflammatory markers are increased during vaso-occlusive crisis (VOC) in adult patients with sickle cell anemia (SCA), but this is not clear in clinical steady state. AIM: The present study aims to establish the frequency and intensity of bone pain episodes in adult patients with SCA in clinical steady state and to determine the correlation between different inflammatory markers, other variables including QT dispersion (QTd) and pain frequency and intensity in SCA...
2016: Journal of Blood Medicine
https://www.readbyqxmd.com/read/27829948/increased-hematocrit-during-sodium-glucose-cotransporter-2-inhibitor-therapy-indicates-recovery-of-tubulointerstitial-function-in-diabetic-kidneys
#7
REVIEW
Motoaki Sano, Makoto Takei, Yasuyuki Shiraishi, Yoshihiko Suzuki
Sodium-glucose cotransporter 2 (SGLT2) inhibitors have been attracting attention for cardiovascular as well as antidiabetic effects since the results of the Empagliflozin Cardiovascular Outcome Event Trial in Type 2 Diabetes Mellitus Patients (EMPA-REG OUTCOME Trial) were reported. The hematocrit increases during treatment with SGLT2 inhibitors, which have a diuretic effect but do not cause sufficient hemoconcentration to increase the risk of cerebral infarction. Elevation of the hematocrit during SGLT2 inhibitor therapy is presumed to involve enhancement of erythropoiesis in addition to hemoconcentration...
December 2016: Journal of Clinical Medicine Research
https://www.readbyqxmd.com/read/27829333/immune-status-in-children-with-beta-thalassemia-in-correlation-with-iron-overload-single-center-egyptian-study
#8
Adel Abd Elhaleim Hagag, Mohamed A Elgamsy, Hassan M El-Asy, Rasha M Gamal, Walid N Elshahaby, Enaam S Abd Elbar
BACKGROUND: 'Beta thalassemia is inherited hemoglobin disorder resulting in chronic hemolytic anemia that requires lifelong transfusion therapy'. 'Repeated blood transfusions and RBCs hemolysis are the main causes of iron overload', which in addition to immune abnormalities, are common predisposing factors to infection in patients with thalassemia. Aim of this work was to study immune status including T lymphocyte subsets and serum immunoglobulin levels 'in children with beta- thalassemia in correlation with iron overload'...
November 7, 2016: Endocrine, Metabolic & Immune Disorders Drug Targets
https://www.readbyqxmd.com/read/27818730/effect-of-lead-exposure-on-the-status-of-reticulocyte-count-indices-among-workers-from-lead-battery-manufacturing-plant
#9
Ravibabu Kalahasthi, Tapu Barman
Earlier studies conducted on lead-exposed workers have determined the reticulocyte count (RC) (%), but the parameters of Absolute Reticulocyte Count (ARC), Reticulocyte Index (RI), and Reticulocyte Production Index (RPI) were not reported. This study assessed the effect of lead (Pb) exposure on the status of reticulocyte count indices in workers occupied in lead battery plants. The present cross-sectional study was carried out on 391 male lead battery workers. The blood lead levels (BLL) were determined by using an Atomic Absorption Spectrophotometer...
October 2016: Toxicological Research
https://www.readbyqxmd.com/read/27802215/sickle-cell-anemia-nitric-oxide-related-genetic-modifiers-of-hematological-and-biochemical-parameters
#10
Laura Aguiar, Andreia Matos, Ângela Gil, Conceiçã Afonso, Lígia Braga, Lavinha João, Paula Kjollerstrom, Paula Faustino, Manuel Bicho, Ângela Inácio
BACKGROUND: Sickle cell anemia (SCA) is an inherited blood disorder. SCA patients present clinical and hematologic variability that cannot be only explained by the single mutation in the beta-globin gene. Others genetic modifiers and environmental effects are important for the clinical phenotype. SCA patients present arginine deficiency that contributes to a lower nitric oxide (NO) bioactivity. OBJECTIVE: The aim of this work is to determine the association between hematological and biochemical parameters and genetic variants from eNOS gene, in pediatric SCA patients...
October 14, 2016: Clinical Hemorheology and Microcirculation
https://www.readbyqxmd.com/read/27784383/-analysis-of-lymphocyte-subsets-in-peripheral-blood-of-patients-with-aplastic-anemia-or-hypoplastic-myelodysplastic-syndrome
#11
Yuan-Fei Lv, Zheng-Yu Yan, Nai-Yiao Chen, Jian-Hui Wu, Bin Liu, Guo-Li Wang, Jing Wang
OBJECTIVE: To explore the ratio of lymphocyte subsets in peripheral blood of patients with aplastic anemia (AA) and patients with hypoplastic myelodysplastic syndrome (hypo-MDS) patients and to evaluate their significance. METHODS: The clinical data of 181 cases of AA and 111 cases of hypo-MDS from January 2008 to December 2014 were collected from Blood Diseases Hospital of Chinese academy of medical sciences, and then the differences of lymphocyte subsets and its effect in 2 groups were analyzed...
October 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/27774434/fatal-liver-and-bone-marrow-toxicity-by-combination-treatment-of-dichloroacetate-and-artesunate-in-a-glioblastoma-multiforme-patient-case-report-and-review-of-the-literature
#12
Martin Uhl, Stefan Schwab, Thomas Efferth
A 52-year-old male patient was treated with standard radiochemotherapy with temozolomide for glioblastoma multiforme (GBM). After worsening of his clinical condition, further tumor-specific treatment was unlikely to be successful, and the patient seeked help from an alternative practitioner, who administered a combination of dichloroacetate (DCA) and artesunate (ART). A few days later, the patient showed clinical and laboratory signs of liver damage and bone marrow toxicity (leukopenia, thrombocytopenia). Despite successful restoration of laboratory parameters upon symptomatic treatment, the patient died 10 days after the infusion...
2016: Frontiers in Oncology
https://www.readbyqxmd.com/read/27767968/rise-in-rbc-aggregability-and-concomitant-decrease-in-blood-pressure-10%C3%A2-days-after%C3%A2-injection-of-the-long-acting-erythropoietin-analogue-methoxy-polyethylene-glycol-epoetin-%C3%AE-mircera%C3%A2
#13
Joré Céline, Brun Jean-Frédéric, Varlet-Marie Emmanuelle
Erythropoietin (EPO) is a major regulator of blood viscosity. Its long lasting action analogue methoxy polyethylene glycol-epoetin-β (MIRCERA®) seems to be also employed in modern doping. We took the opportunity of a study aiming at developing a detection of recent MIRCERATM injection in the context of doping detection to assess the effects of this EPO analogue on red blood cells (RBC) aggregation. A single dose 200 μg of MIRCERA® was injected to 10 male volunteers and blood samplings were drawn over 24 days...
October 17, 2016: Clinical Hemorheology and Microcirculation
https://www.readbyqxmd.com/read/27755775/spurious-reticulocyte-profiles-in-a-dog-with-babesiosis
#14
Laetitia Piane, Marie-Laure Théron, Marcel Aumann, Catherine Trumel
A 9-year-old, female Maltese dog was referred to the Veterinary School of Toulouse with a 2-day history of anorexia and weakness. On clinical examination, the dog had hyperthermia (39.7°C), abdominal discomfort, and polypnea. Significant laboratory findings included pigmenturia, hyperbilirubinemia, hypercreatininemia, hyperfibrinogenemia, abnormal Snap canine pancreas-specific lipase, and pancytopenia with a nonregenerative anemia. A peripheral blood smear revealed numerous intraerythrocytic large Babesia but no polychromasia...
October 18, 2016: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/27713998/diagnosis-of-iron-deficiency-anemia-using-density-based-fractionation-of-red-blood-cells
#15
Jonathan W Hennek, Ashok A Kumar, Alex B Wiltschko, Matthew R Patton, Si Yi Ryan Lee, Carlo Brugnara, Ryan P Adams, George M Whitesides
Iron deficiency anemia (IDA) is a nutritional disorder that impacts over one billion people worldwide; it may cause permanent cognitive impairment in children, fatigue in adults, and suboptimal outcomes in pregnancy. IDA can be diagnosed by detection of red blood cells (RBCs) that are characteristically small (microcytic) and deficient in hemoglobin (hypochromic), typically by examining the results of a complete blood count performed by a hematology analyzer. These instruments are expensive, not portable, and require trained personnel; they are, therefore, unavailable in many low-resource settings...
October 5, 2016: Lab on a Chip
https://www.readbyqxmd.com/read/27705902/height-indicates-hematopoietic-capacity-in-elderly-japanese-men
#16
Yuji Shimizu, Shimpei Sato, Jun Koyamatsu, Hirotomo Yamanashi, Mako Nagayoshi, Koichiro Kadota, Takahiro Maeda
Previously, we reported that height is an indicator of the capacity of vascular repair in elderly men, especially hypertensive men. On the other hand, hemoglobin could act as a possible biochemical index of hypertension-induced vascular damage. However, no studies have clarified the correlation between height and hematopoietic activity. We conducted a cross-sectional study of 249 men aged 65-69 undergoing a general health check-up. Reticulocyte was used to evaluate hematopoietic activity. Because hemoglobin concentration should influence hematopoietic activity, analyses stratified by hemoglobin level were performed...
October 4, 2016: Aging
https://www.readbyqxmd.com/read/27704349/effect-of-preliminary-injection-of-bone-marrow-cell-total-rna-on-erythropoiesis-recovery-dynamics-in-rats-exposed-to-an-acute-%C3%AE-irradiation
#17
N M Gevorkyan, N V Tishevskaya, A A Bolotov
Total RNA from the bone marrow of healthy donor rats was injected to experimental rats 6 h, 2 h, or 30 min prior to a single γ-irradiation in the sublethal dose of 6 Gy. Injection total RNA 30 min prior to the exposure most effectively restored erythropoiesis in experimental animals. In 5 days, reticulocyte count in these animals 30-fold surpassed the control (injection of 0.9% NaCl). In 12 days, the content of new erythroblastic islands in the bone marrow in rats injected with the total RNA 2 h or 30 min prior to irradiation increased significantly and erythropoiesis recovery activation was observed...
September 2016: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/27701759/spurious-reticulocyte-profiles-in-dogs-with-large-form-babesiosis-a-retrospective-study
#18
Laetitia Piane, Karen M Young, Lena Giraud, Nathalie Bourges-Abella, Catherine Trumel
BACKGROUND: Erroneously high reticulocyte counts (pseudoreticulocytosis) have been reported in dogs with leukemia. Pseudoreticulocytosis and an abnormal reticulocyte profile were observed in a dog with large form babesiosis presented at our institution. OBJECTIVES: The aims of this retrospective study were to determine if dogs with babesiosis and other dogs had abnormal reticulocyte profiles, and to correlate these profiles with the primary diagnosis. METHODS: All canine CBCs obtained with the Sysmex XT-2000iV or Procyte DX were reviewed...
October 4, 2016: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/27699249/a-xenograft-model-of-macrophage-activation-syndrome-amenable-to-anti-cd33-and-anti-il-6r-treatment
#19
Mark Wunderlich, Courtney Stockman, Mahima Devarajan, Navin Ravishankar, Christina Sexton, Ashish R Kumar, Benjamin Mizukawa, James C Mulloy
Transgenic expression of key myelosupportive human cytokines in immune-deficient mice corrects for the lack of cross-species activities of stem cell factor (SCF), IL-3, and GM-CSF. When engrafted with human umbilical cord blood (UCB), these triple-transgenic mice produce BM and spleen grafts with much higher myeloid composition, relative to nontransgenic controls. Shortly after engraftment with UCB, these mice develop a severe, fatal macrophage activation syndrome (MAS) characterized by a progressive drop in rbc numbers, increased reticulocyte counts, decreased rbc half-life, progressive cytopenias, and evidence of chronic inflammation, including elevated human IL-6...
September 22, 2016: JCI Insight
https://www.readbyqxmd.com/read/27686852/clinical-course-of-two-children-with-unstable-hemoglobins-the-effect-of-hydroxyurea-therapy
#20
Harriët M Loovers, Nienke Tamminga, André B Mulder, Rienk Y J Tamminga
Case reports on the effect of hydroxyurea (HU) therapy for unstable hemoglobins (Hbs) are sparse; only three adult cases have been reported. We report for the first time on the effect of HU therapy in children carrying unstable Hbs. The first case concerns a female child with a familial history of chronic hemolytic anemia. She was diagnosed with Hb Volga (HBB: c.83C>A) at the age of 7 months. At age 6, treatment options were reconsidered due to increasing fatigue and decreasing Hb concentration. The second case also concerns a female child with chronic hemolytic anemia and icterus since the age of 5...
September 30, 2016: Hemoglobin
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