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https://www.readbyqxmd.com/read/29773104/-knockdown-of-msx2-gene-inhibits-the-expression-of-enamel-matrix-proteins-and-the-enamel-mineralization-in-mouse-ameloblasts
#1
Hao Liu, Jianping Jiang, Qi Gong, Huanbing Tian, Sheng Wang, Juanjuan Zhang, Zhifang Pan, Xiaoying Liu
Objective To study the effect of muscle segment homeodomain homeobox 2 (MSX2) on the expression of enamel matrix protein and the formation of enamel. Methods Immunohistochemical staining was used to detect the expression of MSX2 in mouse tooth embryos and its localization in ameloblasts. The short hairpin RNA (shRNA) of the MSX2 gene was designed and synthesized, and then the annealed double stranded DNA was constructed into the pGMLV-SC5 RNAi lentivirus vector, and finally it was packaged with lentivirus. The lentivirus was used to infect ameloblasts...
March 2018: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/29755616/histological-and-immunological-characteristics-of-the-junctional-epithelium
#2
REVIEW
Masanori Nakamura
The continuity of epithelial tissue is collapsed by tooth eruption. The junctional epithelium (JE) is attached to the tooth surface by hemidesmosomes, which constitutes the front-line defense against periodontal bacterial infection. JE constitutively expresses intercellular adhesion molecule-1 (ICAM-1), and neutrophils and lymphocytes penetrate into JE via interaction between ICAM-1 and LFA-1 expressed on the surface of these migrating cells. JE also expresses cytokines and chemokines. These functions of JE are maintained even in germ-free condition...
May 2018: Japanese Dental Science Review
https://www.readbyqxmd.com/read/29725259/expanding-the-oro-dental-and-mutational-spectra-of-kabuki-syndrome-and-expression-of-kmt2d-and-kdm6a-in-human-tooth-germs
#3
Thantrira Porntaveetus, Mushriq F Abid, Thanakorn Theerapanon, Chalurmpon Srichomthong, Atsushi Ohazama, Katsushige Kawasaki, Maiko Kawasaki, Kanya Suphapeetiporn, Paul T Sharpe, Vorasuk Shotelersuk
Kabuki syndrome is a rare genetic disorder characterized by distinct dysmorphic facial features, intellectual disability, and multiple developmental abnormalities. Despite more than 350 documented cases, the oro-dental spectrum associated with kabuki syndrome and expression of KMT2D (histone-lysine N-methyltransferase 2D) or KDM6A (lysine-specific demethylase 6A) genes in tooth development have not been well defined. Here, we report seven unrelated Thai patients with Kabuki syndrome having congenital absence of teeth, malocclusion, high-arched palate, micrognathia, and deviated tooth shape and size...
2018: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/29720250/efficient-induction-of-functional-ameloblasts-from-human-keratinocyte-stem-cells
#4
Xuefeng Hu, Jyh-Wei Lee, Xi Zheng, Junhua Zhang, Xin Lin, Yingnan Song, Bingmei Wang, Xiaoxiao Hu, Hao-Hueng Chang, Yiping Chen, Chun-Pin Lin, Yanding Zhang
BACKGROUND: Although adult human tissue-derived epidermal stem cells are capable of differentiating into enamel-secreting ameloblasts and forming teeth with regenerated enamel when recombined with mouse dental mesenchyme that possesses odontogenic potential, the induction rate is relatively low. In addition, whether the regenerated enamel retains a running pattern of prism identical to and acquires mechanical properties comparable with human enamel indeed warrants further study. METHODS: Cultured human keratinocyte stem cells (hKSCs) were treated with fibroblast growth factor 8 (FGF8) and Sonic hedgehog (SHH) for 18 h or 36 h prior to being recombined with E13...
May 2, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29670257/directed-reprogramming-of-comprehensively-characterized-dental-pulp-stem-cells-extracted-from-natal-tooth
#5
Rishikaysh V Pisal, Jakub Suchanek, Richard Siller, Tomas Soukup, Hana Hrebikova, Ales Bezrouk, David Kunke, Stanislav Micuda, Stanislav Filip, Gareth Sullivan, Jaroslav Mokry
The aim of this study was to extensively characterise natal dental pulp stem cells (nDPSC) and assess their efficiency to generate human induced pluripotent stem cells (hiPSC). A number of distinguishing features prompted us to choose nDPSC over normal adult DPSC, in that they differed in cell surface marker expression and initial doubling time. In addition, nDPSC expressed 17 out of 52 pluripotency genes we analysed, and the level of expression was comparable to human embryonic stem cells (hESC). Ours is the first group to report comprehensive characterization of nDPSC followed by directed reprogramming to a pluripotent stem cell state...
April 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29615564/sonic-hedgehog-signaling-and-development-of-the-dentition
#6
REVIEW
Maisa Seppala, Gareth J Fraser, Anahid A Birjandi, Guilherme M Xavier, Martyn T Cobourne
Sonic hedgehog (Shh) is an essential signaling peptide required for normal embryonic development. It represents a highly-conserved marker of odontogenesis amongst the toothed vertebrates. Signal transduction is involved in early specification of the tooth-forming epithelium in the oral cavity, and, ultimately, in defining tooth number within the established dentition. Shh also promotes the morphogenetic movement of epithelial cells in the early tooth bud, and influences cell cycle regulation, morphogenesis, and differentiation in the tooth germ...
May 31, 2017: Journal of Developmental Biology
https://www.readbyqxmd.com/read/29511365/scaps-regulate-differentiation-of-dfscs-during-tooth-root-development-in-swine
#7
Xiaoshan Wu, Lei Hu, Yan Li, Yang Li, Fu Wang, Ping Ma, Jinsong Wang, Chunmei Zhang, Canhua Jiang, Songlin Wang
The tooth root transmits and balances occlusal forces through the periodontium to the alveolar bone. The periodontium, including the gingiva, the periodontal ligament, the cementum and the partial alveolar bone, derives from the dental follicle (DF), except for the gingiva. In the early developmental stages, the DF surrounds the tooth germ as a sphere and functions to promote tooth eruption. However, the morphological dynamics and factors regulating the differentiation of the DF during root elongation remain largely unknown...
2018: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/29498025/dental-stem-cells-in-bone-tissue-engineering-current-overview-and-challenges
#8
Pinar Ercal, Gorke Gurel Pekozer, Gamze Torun Kose
The treatment of bone that is impaired due to disease, trauma or tumor resection creates a challenge for both clinicians and researchers. Critical size bone defects are conventionally treated with autografts which are associated with risks such as donor site morbidity and limitations like donor shortage. Bone tissue engineering has become a promising area for the management of critical size bone defects by the employment of biocompatible materials and the discovery of novel stem cell sources. Mesenchymal stem cells (MSCs) can be isolated with ease from various dental tissues including dental pulp stem cells, stem cells from apical papilla, dental follicle stem cells, stem cells from human exfoliated deciduous teeth, periodontal ligament stem cells, gingival stem cells and tooth germ derived stem cells...
March 2, 2018: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29489425/bone-morphogenetic-protein-2-coordinates-early-tooth-mineralization
#9
Z Malik, M Alexiou, B Hallgrimsson, A N Economides, H U Luder, D Graf
Formation of highly organized dental hard tissues is a complex process involving sequential and ordered deposition of an extracellular scaffold, followed by its mineralization. Odontoblast and ameloblast differentiation involves reciprocal and sequential epithelial-mesenchymal interactions. Similar to early tooth development, various Bmps are expressed during this process, although their functions have not been explored in detail. Here, we investigated the role of odontoblast-derived Bmp2 for tooth mineralization using Bmp2 conditional knockout mice...
February 1, 2018: Journal of Dental Research
https://www.readbyqxmd.com/read/29481561/differential-tissue-growth-and-cell-adhesion-alone-drive-early-tooth-morphogenesis-an-ex-vivo-and-in-silico-study
#10
Miquel Marin-Riera, Jacqueline Moustakas-Verho, Yoland Savriama, Jukka Jernvall, Isaac Salazar-Ciudad
From gastrulation to late organogenesis animal development involves many genetic and bio-mechanical interactions between epithelial and mesenchymal tissues. Ectodermal organs, such as hairs, feathers and teeth are well studied examples of organs whose development is based on epithelial-mesenchymal interactions. These develop from a similar primordium through an epithelial folding and its interaction with the mesenchyme. Despite extensive knowledge on the molecular pathways involved, little is known about the role of bio-mechanical processes in the morphogenesis of these organs...
February 2018: PLoS Computational Biology
https://www.readbyqxmd.com/read/29480622/primordial-odontogenic-tumor-subepithelial-expression-of-syndecan-1-and-ki-67-suggests-origin-during-early-odontogenesis
#11
R Bologna-Molina, T Mikami, V Pereira-Prado, G Tapia-Repetto, F R Pires, R Carlos, A Mosqueda-Taylor
Primordial odontogenic tumor (POT) is composed of variably cellular myxoid connective tissue, surrounded by cuboidal to columnar odontogenic epithelium resembling the inner epithelium of the enamel organ, which often invaginates into the underlying connective tissue. The tumor is delimited at least partially by a thin fibrous capsule. It derives from the early stages of tooth development. Syndecan-1 is a heparan sulfate proteoglycan that has a physiological role in several cellular functions, including maintenance of the epithelial architecture, cell-to-cell adhesion and interaction of cells with extracellular matrix, and with diverse growth factors, stimulating cell proliferation...
March 2018: Oral Diseases
https://www.readbyqxmd.com/read/29476681/expression-of-hmlh1-and-hmsh2-proteins-in-ameloblastomas-and-tooth-germs
#12
R Bologna-Molina, V Pereira-Prado, C Sánchez-Romero, G Tapia-Repetto, S Soria, M Hernandez, R Gónzalez-Gónzalez, M Molina-Frechero, T Mikami
BACKGROUND: Mismatch repair proteins (MMRPs) are a group of nuclear enzymes that participate in the repair of base mismatches that occur during DNA replication in all proliferating cells. The most studied MMRPs are hMSH2 and hMLH1, which are known to be highly expressed in normal tissues. A loss of MMRPs leads to the accumulation of DNA replication errors in proliferating cells. Ki-67 is a biomarker regarded to be the gold-standard tool for determining cell proliferation by immunohistochemical methods...
March 1, 2018: Medicina Oral, Patología Oral y Cirugía Bucal
https://www.readbyqxmd.com/read/29457254/primordial-odontogenic-tumour-report-of-two-cases
#13
Asma Almazyad, Chia-Cheng Li, Roberto O C Tapia, Javier P Robertson, David Collette, Sook-Bin Woo
AIMS: Primordial odontogenic tumour (POT) is a rare mixed odontogenic neoplasm that is composed of primitive ectomesenchyme resembling dental papilla, surfaced by odontogenic epithelium resembling inner enamel epithelium, without hard tissue formation. Most reported cases have presented in the posterior mandible as a well-demarcated radiolucency associated with an unerupted tooth in the first two decades of life. The aim of this report is to describe the clinicopathological features of two more cases of POT...
February 19, 2018: Histopathology
https://www.readbyqxmd.com/read/29430872/semaphorin-3a-receptor-inhibitor-as-a-novel-therapeutic-to-promote-innervation-of-bioengineered-teeth
#14
Sabine Kuchler-Bopp, Dominique Bagnard, Michael Van-Der-Heyden, Ysia Idoux-Gillet, Marion Strub, Hervé Gegout, Hervé Lesot, Nadia Benkirane-Jessel, Laetitia Keller
The sensory innervation of the dental pulp is essential for tooth function and protection. It is mediated by axons originating from the trigeminal ganglia and is spatio-temporally regulated. We have previously shown that the innervation of bioengineered teeth can be achieved only under immunosuppressive conditions. The aim of this study was to develop a model to determine the role of Semaphorin 3A (Sema3A) in the innervation of bioengineered teeth. We first analysed innervation of the dental pulp of mandibular first molars in newborn (postnatal day 0: PN0) mice deficient for Sema3A (Sema3A-/- ), a strong inhibitor of axon growth...
April 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29428727/establishment-of-mouse-gingival-junctional-epithelial-cell-line-using-a-bioengineered-tooth-system
#15
Tatsuaki Seki, Ryo Aizawa, Junichi Tanaka, Sara Yajima-Himuro, Mayu Kato, Keisuke Tanaka, Kenji Mishima, Matsuo Yamamoto
Junctional epithelium (JE), one of the constituents of periodontal tissue, has several unique features to prevent bacterial infection. However, the molecular mechanisms of these cells remain to be completely elucidated because there has been no JE cell line to date. We have succeeded in isolating JE cells expressing green fluorescent protein (GFP) by using a bioengineered tooth technique in mice. The gene expressions of GFP-positive JE cells, isolated from around the erupted bioengineered teeth using flow cytometry, were analyzed by RNA sequencing...
February 26, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29399866/tooth-germ-initiation-patterns-in-a-developing-dentition-an-in-vivo-study-of-xenopus-laevis-tadpoles
#16
Denis O Lamoureux, Aaron R H LeBlanc, Michael W Caldwell
The transparency of soft tissue in Xenopus laevis tadpoles and the anterior-posterior orientation of their developing tooth germs in the upper jaw offer a unique opportunity for the in vivo charting of the first 15-20 days of the developing dentition. Twenty-two X. laevis tadpoles were anesthetized daily and their mouths opened to record the first appearance, position, and development of tooth germs in the upper jaw. The initiation patterns revealed considerable variability between animals, and even between the jaw quadrants in the same animal...
May 2018: Journal of Morphology
https://www.readbyqxmd.com/read/29396644/expression-and-localization-of-cramp-in-rat-tooth-germ-and-during-reparative-dentin-formation
#17
Kanji Horibe, Akihiro Hosoya, Toru Hiraga, Hiroaki Nakamura
OBJECTIVES: Cathelicidin-related antimicrobial peptide (CRAMP) is an antimicrobial peptide in mice and rats homologous to LL-37 in humans. In addition to its antibacterial activity, CRAMP has various physiological functions by binding to formyl peptide receptor 2 (FPR2). However, the role of these peptides in teeth is unknown. Therefore, we investigated the role of CRAMP and FPR2 in tooth development, reparative dentin formation, and defense response. MATERIAL AND METHODS: First, we examined the localization of CRAMP and FPR2 during tooth development by immunohistochemical analysis...
February 2, 2018: Clinical Oral Investigations
https://www.readbyqxmd.com/read/29277139/perception-and-practices-of-nomadic-women-in-a-rural-community-in-southwestern-nigeria-to-their-children-s-oral-health
#18
Olubunmi Olusola Bankole, Folake Barakat Lawal, Abimbola Balogun
Introduction Nomads move from one settlement to another, thus it has been difficult to have adequate documentation about their oral health and that of their children. Aim To investigate the perception and practices of nomadic Fulani women toward their children's oral health. Methodology A cross-sectional study was conducted among 197 Fulani women using a structured interviewer administered questionnaire. Results Perceived causes of tooth decay included tooth germs/worms (23.9%) and sugar (5.1%), and 32.0%, 15...
October 2017: International Quarterly of Community Health Education
https://www.readbyqxmd.com/read/29275570/-three-dimensional-printed-miniplate-used-for-maxillary-protraction
#19
S R Liang, F Wang, D Q Zhou, S Chang, Y X Bai
The miniplate was designed and three-dimensional (3D) printed according to the positions of roots and tooth germs and then it was used as skeletal anchorage to protract the maxilla. The maxilla moved forward obviously after treatment. Custom designed and 3D printed miniplate could be used for maxillary protraction.
December 9, 2017: Zhonghua Kou Qiang Yi Xue za Zhi, Zhonghua Kouqiang Yixue Zazhi, Chinese Journal of Stomatology
https://www.readbyqxmd.com/read/29249735/longitudinal-observation-of-maxillary-sinus-bony-bridges-and-septa-in-childhood
#20
Munetaka Naitoh, Hirofumi Watanabe, Kazuhito Yoshida, Hisashi Hayashi, Kenichi Gotoh, Eiichiro Ariji
The rate of septum presence in the maxillary sinus has been reported to be over 30%. It was considered that a bony bridge might change to a maxillary sinus septum with growth in a previous study using dry child skulls. In the present investigation, maxillary sinus bony bridges and septa were longitudinally observed using computed tomography (CT). Multislice CT was performed in three patients. A bony bridge was defined as a bony structure between the maxillary sinus wall and dental germ. Also, a septum was defined as a pointed bony structure in the inferior wall of the maxillary sinus...
2017: Okajimas Folia Anatomica Japonica
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