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https://www.readbyqxmd.com/read/28782241/a-novel-mutation-of-adenomatous-polyposis-coli-apc-gene-results-in-the-formation-of-supernumerary-teeth
#1
Fang Yu, Wenping Cai, Beizhan Jiang, Laijun Xu, Shangfeng Liu, Shouliang Zhao
Supernumerary teeth are teeth that are present in addition to normal teeth. Although several hypotheses and some molecular signalling pathways explain the formation of supernumerary teeth, but their exact disease pathogenesis is unknown. To study the molecular mechanisms of supernumerary tooth-related syndrome (Gardner syndrome), a deeper understanding of the aetiology of supernumerary teeth and the associated syndrome is needed, with the goal of inhibiting disease inheritance via prenatal diagnosis. We recruited a Chinese family with Gardner syndrome...
August 7, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28776953/establishment-of-oral-bacterial-communities-in-germ-free-mice-and-the-influence-of-recipient-age
#2
Yingying Wu, Caroline Westwater, E Xiao, Jôice Dias Corrêa, Wenmei Xiao, Dana T Graves
The acquisition of the oral microbiome is a complex process. We examined how the timing of microbial exposure alters bacterial colonization of the tooth surface. Germ-free mice were conventionalized by exposure to specific pathogen free (SPF) mice to acquire a commensal microbiome over three distinct four week periods, 0-4 weeks of age (Conv0-4w), 4-8 weeks (Conv4-8w), or 8-12 weeks (Conv8-12w). Bacterial DNA was extracted from the tooth surface and analyzed by 16S rDNA sequencing. Total bacteria and inflammatory cytokine expression in gingiva were determined by quantitative real-time PCR...
August 4, 2017: Molecular Oral Microbiology
https://www.readbyqxmd.com/read/28762352/a-comparative-study-to-associate-the-presence-of-neonatal-line-in-deciduous-teeth-of-infants-with-the-occurrence-of-live-birth
#3
Shruti Srinivasan, Prashanth Sadashiva Murthy, Seema Deshmukh, N M Shamsundar
CONTEXT: The prominent striae of retzius corresponding to the occurrence of live birth is known as the neonatal line (NNL). The very presence of this line indicates the exposure of the infant to the stressful event of birth. AIMS: The study was aimed at locating the NNL in the developing primary teeth of the neonate and associating the presence of NNL to the occurrence of live birth. MATERIALS AND METHODS: The donated bodies of the neonates were taken, and a surgical incision was made along the crest of the alveolar ridge in the maxillary anterior region, enabling the careful removal of the tooth germs from within the jaw segment...
July 2017: Journal of the Indian Society of Pedodontics and Preventive Dentistry
https://www.readbyqxmd.com/read/28756608/inhibition-of-chondroitin-sulfate-glycosaminoglycans-incorporation-affected-odontoblast-differentiation-in-cultured-embryonic-mouse-molars
#4
Lipei Liu, Weiting Chen, Lefeng Li, Fangfang Xu, Beizhan Jiang
Chondroitin sulfate proteoglycan (CSPG) is an important component of extracellular matrix (ECM), it is composed of a core protein and one or more chondroitin sulfate glycosaminoglycan side chains (CS-GAGs). To investigate the roles of its CS-GAGs in dentinogenesis, the mouse mandibular first molar tooth germs at early bell stage were cultivated with or without β-xyloside. As expected, the CS-GAGs were inhibited on their incorporation to CSPGs by β-xyloside, accompanied by the change of morphology of the cultured tooth germs...
July 29, 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28734144/expression-of-planar-cell-polarity-genes-during-mouse-tooth-development
#5
Nobuko Obara, Yuko Suzuki, Kazuharu Irie, Shunichi Shibata
OBJECTIVE: Planar cell polarity (PCP) refers to the cell polarity across the tissue plane and controls various cell behaviors and structures. Although the expression of several PCP signaling components has been detected in tooth germs, knowledge of the gene expression patterns of these PCP components during tooth development remains incomplete. The aim of this study is to characterize the temporal and spatial changes in PCP gene expression during tooth development. DESIGN: Expression of Celsr1 and 2, Fzd3 and 6, Vangl1 and 2, and Dvl1-3 genes was analyzed in mouse molar germs from the bud to the bell stage using in situ hybridization...
July 14, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28733464/lineage-tracing-of-epithelial-cells-in-developing-teeth-reveals-two-strategies-for-building-signaling-centers
#6
Wei Du, Jimmy Kuang-Hsien Hu, Wen Du, Ophir D Klein
An important event in organogenesis is the formation of signaling centers, which are clusters of growth-factor secreting cells. In the case of tooth development, sequentially formed signaling centers known as the initiation knot (IK) and the enamel knot (EK) regulate morphogenesis. However, despite the importance of signaling centers, their origin, as well as the fate of the cells composing them, remain open questions. Here, using lineage tracing of distinct epithelial populations, we found that the EK of the mouse incisor is derived de novo from a group of SRY-box 2 (Sox2)-expressing cells in the posterior half of the tooth germ...
July 21, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28729805/dental-treatment-considerations-for-a-pediatric-patient-with-incontinentia-pigmenti-bloch-sulzberger-syndrome
#7
Amy Yi-Ling Chen, Kevin Chen
Incontinentia pigmenti (IP) is a uncommon gene disorder, heritage with X-linked dominant mode. IP patients have a characteristic dentition varying from marked hypodontia to delayed eruption and conical crowns on both dentitions. A 5½-year-old girl, whose mother and younger sister were also diagnosed with IP, has the whirling-like pigmented skin lesion over her trunk and four extremities. Four primary teeth and multiple permanent tooth germs were found to be congenital missing. Dental considerations of further treatment were discussed with her parents including the preservation of primary molars, possible interim prosthesis in mixed or permanent dentition, full mouth rehabilitation with orthodontic and prosthodontic combined treatment, and implant therapy in adulthood...
April 2017: European Journal of Dentistry
https://www.readbyqxmd.com/read/28706494/rna-sequencing-analyses-demonstrate-the-involvement-of-canonical-transient-receptor-potential-channels-in-rat-tooth-germ-development
#8
Jun Yang, Wenping Cai, Xi Lu, Shangfeng Liu, Shouliang Zhao
Tooth development depends on multiple molecular interactions between the dental epithelium and mesenchyme, which are derived from ectodermal and ectomesenchymal cells, respectively. We report on a systematic RNA sequencing analysis of transcriptional expression levels from the bud to hard tissue formation stages of rat tooth germ development. We found that GNAO1, ENO1, EFNB1, CALM1, SIAH2, ATP6V0A1, KDELR2, GTPBP1, POLR2C, SORT1, and members of the canonical transient receptor potential (TRPC) channel family are involved in tooth germ development...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28704648/diabetes-enhances-il-17-expression-and-alters-the-oral-microbiome-to-increase-its-pathogenicity
#9
E Xiao, Marcelo Mattos, Gustavo Henrique Apolinário Vieira, Shanshan Chen, Jôice Dias Corrêa, Yingying Wu, Mayra Laino Albiero, Kyle Bittinger, Dana T Graves
Diabetes is a risk factor for periodontitis, an inflammatory bone disorder and the greatest cause of tooth loss in adults. Diabetes has a significant impact on the gut microbiota; however, studies in the oral cavity have been inconclusive. By 16S rRNA sequencing, we show here that diabetes causes a shift in oral bacterial composition and, by transfer to germ-free mice, that the oral microbiota of diabetic mice is more pathogenic. Furthermore, treatment with IL-17 antibody decreases the pathogenicity of the oral microbiota in diabetic mice; when transferred to recipient germ-free mice, oral microbiota from IL-17-treated donors induced reduced neutrophil recruitment, reduced IL-6 and RANKL, and less bone resorption...
July 12, 2017: Cell Host & Microbe
https://www.readbyqxmd.com/read/28686984/influence-of-stro-1-selection-on-osteogenic-potential-of-human-tooth-germ-derived-mesenchymal-stem-cells
#10
Pinar Ercal, Gorke G Pekozer, Osman Z Gumru, Gamze T Kose, Mustafa Ramazanoglu
Mesenchymal stem cells derived from the human tooth germ (hTGSCs) are a heterogeneous cell population that can differentiate into osteogenic, neurogenic, and adipogenic lineages. The aim of this study was to compare the osteogenic differentiation capacity of STRO-1 positive (STRO-1+) hTGSCs and unsorted heterogeneous hTGSCs and to establish if STRO-1+ cells are more committed to osteogenic differentiation. HTGSCs were isolated from impacted third molar tooth germ tissues of adolescents, and a subpopulation of STRO-1+ hTGSCs was obtained by fluorescence-activated cell sorting...
June 27, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28683410/different-expression-patterns-of-lin28-and-lin28b-in-mouse-molar-development
#11
Ning Dong, Yan Liu, Tiantian Zhang, Lin Zhao, Jiangang Tian, Jianping Ruan
OBJECTIVE: The RNA-binding proteins Lin28 and Lin28b are expressed in many developing tissues and are involved in the biosynthesis of the microRNA let-7 family and embryogenesis processes. However, their roles in mammalian tooth development remain ill-defined. DESIGN: The spatiotemporal expressions of Lin28 and Lin28b during mouse molar odontogenesis from day E11.5 to P21 were examined through immunohistochemistry and western blot analysis. RESULTS: Both Lin28 and Lin28b were initially expressed in dental epithelium, but the expression patterns varied thereafter...
June 23, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28681425/localization-of-relm-%C3%AE-fizz2-is-associated-with-cementum-formation
#12
Akihiro Hosoya, Akira Takahama, Hiroaki Nakamura
Resistin-like molecule-β/found in inflammatory zone 2 (RELM-β/FIZZ2) is a cysteine-rich secretory protein that is localized in the epithelium of the gastrointestinal tract and lung alveoli. Previous reports have suggested that this protein regulates glucose metabolism and inflammation. In the present study, to analyze the involvement of RELM-β/FIZZ2 in tooth development, we immunohistochemically examined the localization of RELM-β/FIZZ2 in tooth germs of embryonic days (E) 15-20 and postnatal days (P) 7-42 rats...
July 6, 2017: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
https://www.readbyqxmd.com/read/28677541/generation-of-induced-pluripotent-stem-cell-ipsc-line-from-a-36-year-old-charcot-marie-tooth-disease-patient-with-gjb1-mutation-cmtx
#13
Daryeon Son, Phil Jun Kang, Wonjin Yun, Seungkwon You
Charcot-Marie-Tooth disease (CMTX) is inherited neurological disorder caused by gap junction beta 1 gene (GJB1) mutation. We generated induced pluripotent stem cell (iPSC) line from 36-year-old CMTX disease patient by electroporation of skin fibroblasts with episomal vectors encoding OCT4, SOX2, KLF4, L-MYC, LIN28 and shRNA-p53. Established iPSCs expressed various pluripotency markers, had differentiation potential of three germ layers in vitro, had normal karyotype and retained GJB1 mutation. This CMT patient-derived iPSC line could be useful in vitro tool for CMTX research as disease modeling and drug development...
May 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28621286/endodontic-management-of-dilacerated-maxillary-central-incisor-fused-to-a-supernumerary-tooth-using-cone-beam-computed-tomography-an-unusual-clinical-presentation
#14
Thilla S Vinothkumar, Deivanayagam Kandaswamy, Ganesh Arathi, Sathishkumar Ramkumar, Gnanasekaran Felsypremila
AIM: The purpose of this report is to present a case of endodontic management of a dilacerated maxillary central incisor fused to supernumerary tooth unusually appearing as a labial tubercle using cone beam computed tomography (CBCT) as a diagnostic aid. BACKGROUND: Anterior teeth are commonly encountered with aberrant anatomical variations in the crown, number of roots, and root canals. Fusion is an infrequent developmental abnormality in shape of the tooth caused by the union of two adjacent tooth germs...
June 1, 2017: Journal of Contemporary Dental Practice
https://www.readbyqxmd.com/read/28605600/activin-and-bmp4-signaling-converge-on-wnt-activation-during-odontogenesis
#15
H-J E Kwon, S Jia, Y Lan, H Liu, R Jiang
Previous studies show that both activin and Bmp4 act as crucial mesenchymal odontogenic signals during early tooth development. Remarkably, mice lacking activin-βA ( Inhba(-/-)) and mice with neural crest-specific inactivation of Bmp4 ( Bmp4(ncko/ncko)) both exhibit bud-stage developmental arrest of the mandibular molar tooth germs while their maxillary molar tooth germs completed morphogenesis. In this study, we found that, whereas expression of Inhba and Bmp4 in the developing tooth mesenchyme is independent of each other, Bmp4(ncko/ncko)Inhba(-/-) compound mutant mice exhibit early developmental arrest of all tooth germs...
June 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28596736/claudin-loss-of-function-disrupts-tight-junctions-and-impairs-amelogenesis
#16
Claire Bardet, Sandy Ribes, Yong Wu, Mamadou Tidiane Diallo, Benjamin Salmon, Tilman Breiderhoff, Pascal Houillier, Dominik Müller, Catherine Chaussain
Claudins are a family of proteins that forms paracellular barriers and pores determining tight junctions (TJ) permeability. Claudin-16 and -19 are pore forming TJ proteins allowing calcium and magnesium reabsorption in the thick ascending limb of Henle's loop (TAL). Loss-of-function mutations in the encoding genes, initially identified to cause Familial Hypomagnesemia with Hypercalciuria and Nephrocalcinosis (FHHNC), were recently shown to be also involved in Amelogenesis Imperfecta (AI). In addition, both claudins were expressed in the murine tooth germ and Claudin-16 knockout (KO) mice displayed abnormal enamel formation...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28574578/the-fam50a-positively-regulates-ameloblast-differentiation-via-interacting-with-runx2
#17
Yuri Kim, Sung-Woong Hur, Byung-Chul Jeong, Sin-Hye Oh, Yun-Chan Hwang, Sun-Hun Kim, Jeong-Tae Koh
Differentiated ameloblasts secret enamel matrix proteins such as amelogenin, ameloblastin, and enamelin. Expression levels of these proteins are regulated by various factors. To find a new regulatory factor for ameloblast differentiation, we performed 2D-PAGE analysis using mouse ameloblast lineage cell line (mALCs) cultured with mineralizing medium. Of identified proteins, family with sequence similarity 50 member A (Fam50a) was significantly increased during differentiation of mALCs. Fam50a protein was also highly expressed in secretory ameloblasts of mouse tooth germs...
June 2, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28562183/extracellular-matrices-for-bone-regeneration-a-literature-review
#18
Alaa Mansour, Mohamed Amine Mezour, Zahi Badran, Faleh Tamimi
The gold standard material for bone regeneration is still autologous bone, a mesenchymal tissue that consists mainly of extracellular matrix (ECM) (90% v/v) and little cellular content (10% v/v). However, the fact that decellularized allogenic bone grafts often present a clinical performance comparable to autologous bone grafts demonstrates the crucial role of ECM in bone regeneration. For long, the mechanism by which bone allografts function was not clear, but recent research has unveiled many unique characteristics of ECM that seem to play a key role in tissue regeneration...
July 12, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28562085/role-of-stro-1-sorting-of-porcine-dental-germ-stem-cells-in-dental-stem-cell-mediated-bone-tissue-engineering
#19
Gorke Gurel Pekozer, Mustafa Ramazanoglu, Karl Andreas Schlegel, Fatma Nese Kok, Gamze Torun Kose
Stem cells of dental origin emerged as a new source for the regeneration of tissues with advantages mainly including non-invasive collection procedures and lack of ethical contraversies with their harvest or use. In this study, porcine TGSCs (pTGSCs) were isolated from mandibular third molar tooth germs of 6-month-old domestic pigs. This is the first study that reports the isolation and characterization of TGSCs from porcine third molars and their differentiation depending on STRO-1 expression. PTGSCs were sorted according to their STRO-1 expression as STRO-1(+) and STRO-1(-)...
May 31, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28541773/induction-of-salivary-gland-like-cells-from-dental-follicle-epithelial-cells
#20
Q L Xu, A Furuhashi, Q Z Zhang, C M Jiang, T-H Chang, A D Le
The dental follicle (DF), most often associated with unerupted teeth, is a condensation of ectomesenchymal cells that surrounds the tooth germ in early stages of tooth development. In the present study, we aim to isolate epithelial stem-like cells from the human DF and explore their potential differentiation into salivary gland (SG) cells. We demonstrated the expression of stem cell-related genes in the epithelial components of human DF tissues, and these epithelial progenitor cells could be isolated and ex vivo expanded in a reproducible manner...
August 2017: Journal of Dental Research
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