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https://www.readbyqxmd.com/read/29656256/phosphatase-mediated-bioprecipitation-of-lead-as-pyromorphite-by-achromobacter-xylosoxidans
#1
Jaya Sharma, Kashif Shamim, Santosh Kumar Dubey
Achromobacter xylosoxidans strain SJ11, tolerating up to 4.0 mM lead nitrate, in a defined minimal medium was isolated from the waste of a battery manufacturing industry, Goa, India. Interestingly, it formed white precipitate on exposure to lead nitrate which was also evident from scanning electron micrograph (SEM). Energy dispersive X-ray spectroscopic analysis revealed the presence of lead (48.5% by weight) along with phosphorus and chlorine in the precipitate. Transmission electron microscopy (TEM) of bacterial cells clearly refuted the possibility of intracellular lead uptake confirming extracellular precipitation as a predominant mechanism of lead resistance in this bacterium...
April 12, 2018: Journal of Environmental Management
https://www.readbyqxmd.com/read/29656117/baicalin-modulates-the-treg-teff-balance-to-alleviate-uveitis-by-activating-the-aryl-hydrocarbon-receptor
#2
Wenjie Zhu, Xiaoqing Chen, Jianfeng Yu, Yichen Xiao, Yingqi Li, Shangtao Wan, Wenru Su, Dan Liang
Autoimmune uveitis is a sight-threatening ocular inflammatory disorder. Immunological inflammation is regarded as the key to pathogenesis in autoimmune uveitis. Baicalin, the major bioactive component of Scutellaria baicalensis, possesses immunomodulatory properties. However, the role of baicalin in uveitis and its underlying mechanisms remain unclear. In the current study, we found that baicalin treatment obviously inhibited the intraocular inflammatory process in mice with experimental autoimmune uveitis, along with clear declines in infiltrated inflammatory cells and inflammatory cytokine transcription in the retina and draining lymph nodes...
April 12, 2018: Biochemical Pharmacology
https://www.readbyqxmd.com/read/29656046/developmental-expression-profiles-and-thyroidal-regulation-of-cytokines-during-metamorphosis-in-the-amphibian-xenopus-laevis
#3
Melanie J Gallant, Natacha S Hogan
Early life-stages of amphibians rely on the innate immune system for defense against pathogens. While thyroid hormones (TH) are critical for metamorphosis and later development of the adaptive immune system, the role of TH in innate immune system development is less clear. An integral part of the innate immune response are pro-inflammatory cytokines - effector molecules that allow communication between components of the immune system. The objective of this study was to characterize the expression of key pro-inflammatory cytokines, tumor necrosis factor-α (TNFα), interleukin-1β (IL-1β) and interferon-γ (IFN-γ), throughout amphibian development and determine the impacts of thyroidal modulation on their expression...
April 12, 2018: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/29655915/real-time-monitoring-of-ph-dependent-intracellular-trafficking-of-ovarian-cancer-g-protein-coupled-receptor-1-in-living-leukocytes
#4
Modong Tan, Satoshi Yamaguchi, Motonao Nakamura, Teruyuki Nagamune
G-protein coupled receptors (GPCRs) are involved in many diseases and important biological phenomena; elucidating the mechanisms underlying regulation of their signal transduction potentially provides both novel targets for drug discovery and insight into living systems. A proton-sensing GPCR, ovarian cancer G protein-coupled receptor 1 (OGR1), has been reported to be related to acidosis and diseases that cause tissue acidification, but the mechanism of proton-induced activation of OGR1-mediated signal transduction in acidic conditions remains unclear...
April 11, 2018: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/29655675/eupatilin-suppresses-the-allergic-inflammatory-response-in-vitro-and-in-vivo
#5
Eun-Hye Song, Kyung-Sook Chung, Yun-Mi Kang, Jong-Hyun Lee, Minho Lee, Hyo-Jin An
INTRODUCTION: Eupatilin, a pharmacologically active ingredient found in Artemisia asiatica, has been reported to have anti-oxidative, anti-inflammatory, and anti-apoptotic activities. However, molecular mechanisms underlying its anti-allergic properties are not yet clear. In this study, we investigated the effects of eupatilin on allergic inflammation in phorbol 12-myristate 13-acetate plus calcium ionophore A23187 (PMACI)-stimulated human mast cells and a compound 48/80-induced anaphylactic shock model...
March 15, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/29655560/selected-reaction-monitoring-mass-spectrometry-of-mastitis-milk-reveals-pathogen-specific-regulation-of-bovine-host-response-proteins
#6
Ulrike Kusebauch, Lorenzo E Hernández-Castellano, Stine L Bislev, Robert L Moritz, Christine M Røntved, Emøke Bendixen
Mastitis is a major challenge to bovine health. The detection of sensitive markers for mastitis in dairy herds is of great demand. Suitable biomarkers should be measurable in milk and should report pathogen-specific changes at an early stage to support earlier diagnosis and more efficient treatment. However, the identification of sensitive biomarkers in milk has remained a challenge, in part due to their relatively low concentration in milk. In the present study, we used a selected reaction monitoring (SRM) mass spectrometry approach, which allowed the absolute quantitation of 13 host response proteins in milk for the first time...
April 11, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29655371/platelet-derived-growth-factor-predicts-prolonged-relapse-free-period-in-multiple-sclerosis
#7
Mario Stampanoni Bassi, Ennio Iezzi, Girolama A Marfia, Ilaria Simonelli, Alessandra Musella, Georgia Mandolesi, Diego Fresegna, Patrizio Pasqualetti, Roberto Furlan, Annamaria Finardi, Giorgia Mataluni, Doriana Landi, Luana Gilio, Diego Centonze, Fabio Buttari
BACKGROUND: In the early phases of relapsing-remitting multiple sclerosis (RR-MS), a clear correlation between brain lesion load and clinical disability is often lacking, originating the so-called clinico-radiological paradox. Different factors may contribute to such discrepancy. In particular, synaptic plasticity may reduce the clinical expression of brain damage producing enduring enhancement of synaptic strength largely dependent on neurotrophin-induced protein synthesis. Cytokines released by the immune cells during acute inflammation can alter synaptic transmission and plasticity possibly influencing the clinical course of MS...
April 14, 2018: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/29653380/fibroblast-populated-collagen-lattices-exhibit-opposite-biophysical-conditions-by-fibrin-or-hyaluronic-acid-supplementation
#8
Mario Chopin-Doroteo, Rosa M Salgado-Curiel, José Pérez-González, Benjamín M Marín-Santibáñez, Edgar Krötzsch
Fibrin and hyaluronic acid are important components of the provisional wound matrix. Through interactions with fibroblasts, they provide biophysical cues that regulate the viscoelastic properties of the extracellular matrix. To understand the roles of fibrin and hyaluronic acid in a collagenous environment, we used fibroblast populated collagen lattices (collagen, collagen-fibrin, and collagen-hyaluronic acid). Compared with collagen and collagen-hyaluronic acid cultures, collagen-fibrin cultures showed less contraction, which is correlated with increased elastic (G') and complex (|G*|) moduli, and reduced proportions of dendritic fibroblasts, despite increased αv integrin expression...
March 31, 2018: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/29653293/transcriptional-profiling-of-murine-osteoblast-differentiation-based-on-rna-seq-expression-analyses
#9
Layal Abo Khayal, Johannes Grünhagen, Ivo Provazník, Stefan Mundlos, Uwe Kornak, Peter N Robinson, Claus-Eric Ott
Osteoblastic differentiation is a multistep process characterized by osteogenic induction of mesenchymal stem cells, which then differentiate into proliferative pre-osteoblasts that produce copious amounts of extracellular matrix, followed by stiffening of the extracellular matrix, and matrix mineralization by hydroxylapatite deposition. Although these processes have been well characterized biologically, a detailed transcriptional analysis of murine primary calvaria osteoblast differentiation based on RNA sequencing (RNA-seq) analyses has not previously been reported...
April 10, 2018: Bone
https://www.readbyqxmd.com/read/29653134/the-optimal-ethanol-lock-therapy-regimen-for-treatment-of-biofilm-associated-catheter-infections-results-from-an-in-vitro-study
#10
Beatriz Alonso, María Jesús Pérez-Granda, Ana Rodríguez-Huerta, Carmen Rodríguez, Emilio Bouza, María Guembe
Ethanol-based lock therapy (LT) solutions are used as an alternative to antibiotics for the conservative management of catheter-related bloodstream infection. However, no clear consensus on regimen or dose has been reached. Our objective was to find the ethanol-based lock solution containing a sufficiently low concentration of ethanol for reduction of the metabolic activity of bacterial and fungal biofilms. Using an in vitro model, we tested 3 concentrations of ethanol (25%-40%-70%), with and without 60 IU of heparin, at 6 different time points and against 24-hour preformed biofilms of Staphylococcus aureus ATCC29213, Staphylococcus epidermidis (clinical isolate), Enterococcus faecalis ATCC33186, Candida albicans ATCC14058, and Escherichia coli ATCC25922...
April 10, 2018: Journal of Hospital Infection
https://www.readbyqxmd.com/read/29653046/mechanism-of-action-of-the-cytotoxic-asmarine-alkaloids
#11
Michael J Lambrecht, Jeffery W Kelly, Ryan A Shenvi
The asmarines are a family of cytotoxic natural products whose mechanism of action is unknown. Here we used chemical synthesis to reverse engineer the asmarines and understand the functions of their individual components. We found that the potent asmarine analog "delmarine" arrested the mammalian cell cycle in G1 phase, and that both cell cycle arrest and cytotoxicity was rescued by co-treatment with ferric and ferrous salts. Cellular iron deprivation was clearly indicated by changes in iron-responsive protein markers, and cytotoxicity occurred independent of radical oxygen species (ROS) production...
April 13, 2018: ACS Chemical Biology
https://www.readbyqxmd.com/read/29652829/molecular-and-ultrastructural-mechanisms-underlying-yellow-dwarf-symptom-formation-in-wheat-after-infection-of-barley-yellow-dwarf-virus
#12
Wei Rong, Xindong Wang, Xifeng Wang, Sebastien Massart, Zengyan Zhang
Wheat ( Tritium aestivum L.) production is essential for global food security. Infection of barley yellow dwarf virus-GAV (BYDV-GAV) results in wheat showing leaf yellowing and plant dwarfism symptom. To explore the molecular and ultrastructural mechanisms underlying yellow dwarf symptom formation in BYDV-GAV-infected wheat, we investigated the chloroplast ultrastructure via transmission electron microscopy (TEM), examined the contents of the virus, H₂O₂, and chlorophyll in Zhong8601, and studied the comparative transcriptome through microarray analyses in the susceptible wheat line Zhong8601 after virus infection...
April 13, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29652806/excessive-extracellular-atp-desensitizes-p2y2-and-p2x4-atp-receptors-provoking-surfactant-impairment-ending-in-ventilation-induced-lung-injury
#13
REVIEW
Djo Hasan, Joshua Satalin, Philip van der Zee, Michaela Kollisch-Singule, Paul Blankman, Atsuko Shono, Peter Somhorst, Corstiaan den Uil, Han Meeder, Toru Kotani, Gary F Nieman
Stretching the alveolar epithelial type I (AT I) cells controls the intercellular signaling for the exocytosis of surfactant by the AT II cells through the extracellular release of adenosine triphosphate (ATP) (purinergic signaling). Extracellular ATP is cleared by extracellular ATPases, maintaining its homeostasis and enabling the lung to adapt the exocytosis of surfactant to the demand. Vigorous deformation of the AT I cells by high mechanical power ventilation causes a massive release of extracellular ATP beyond the clearance capacity of the extracellular ATPases...
April 13, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29652170/pulmonary-transplantation-of-human-ipsc-derived-macrophages-ameliorates-pulmonary-alveolar-proteinosis
#14
Christine Happle, Nico Lachmann, Mania Ackermann, Anja Mirenska, Gudrun Göhring, Kathrin Thomay, Adele Mucci, Miriam Hetzel, Torsten Glomb, Takuji Suzuki, Claudia Chalk, Silke Glage, Oliver Dittrich-Breiholz, Bruce Trapnell, Thomas Moritz, Gesine Hansen
Rationale While the transplantation of induced pluripotent stem cell (iPSC)-derived cells harbors enormous potential for the treatment of pulmonary diseases, in vivo data demonstrating clear therapeutic benefits of human iPSC-derived cells in lung disease models is missing. Objective We here tested the therapeutic potential of iPSC-derived macrophages in a humanized disease model of hereditary pulmonary alveolar proteinosis (herPAP). herPAP is caused by a genetic defect of the GM-CSF-receptor, which leads to disturbed macrophage differentiation and protein/surfactant degradation in the lungs subsequently resulting in severe respiratory insufficiency...
April 13, 2018: American Journal of Respiratory and Critical Care Medicine
https://www.readbyqxmd.com/read/29651934/effects-of-curcumin-on-squamous-cell-carcinoma-of-tongue-an-in-vitro-study
#15
Lorenzo Lo Muzio, Fatima Ardito
BACKGROUND: The squamous cell carcinoma of the tongue (TSCC) is the most frequent cancer of oral cavity often characterized by poor prognosis. Conventional therapies are not very efficient and often may cause serious side effects. In this contest, introduction of natural substances as possible adjuvant in the treatment and prevention of cancer is becoming a relevant topic. In fact, curcumin has been used for decades in Chinese traditional medicine for its beneficial effects. Curcumin has anti-cancer properties in many tumors however, its action on the tongue carcinoma is not entirely clear and many other investigations are necessary...
April 12, 2018: Current Topics in Medicinal Chemistry
https://www.readbyqxmd.com/read/29651285/atomic-force-microscopy-of-side-wall-and-septa-peptidoglycan-from-bacillus-subtilis-reveals-an-architectural-remodeling-during-growth
#16
Kang Li, Xiao-Xue Yuan, He-Min Sun, Long-Sheng Zhao, Ruocong Tang, Zhi-Hua Chen, Qi-Long Qin, Xiu-Lan Chen, Yu-Zhong Zhang, Hai-Nan Su
Peptidoglycan is the fundamental structural constituent of the bacterial cell wall. Despite many years of research, the architecture of peptidoglycan is still largely elusive. Here, we report the high-resolution architecture of peptidoglycan from the model Gram-positive bacterium Bacillus subtilis . We provide high-resolution evidence of peptidoglycan architecture remodeling at different growth stages. Side wall peptidoglycan from B. subtilis strain AS1.398 changed from an irregular architecture in exponential growth phase to an ordered cable-like architecture in stationary phase...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29651135/author-correction-the-oncogenic-role-of-col23a1-in-clear-cell-renal-cell-carcinoma
#17
Fujiang Xu, Kun Chang, Jian Ma, Yuanyuan Qu, Huyang Xie, Bo Dai, Hualei Gan, Hailiang Zhang, Guohai Shi, Yao Zhu, Yiping Zhu, Yijun Shen, Dingwei Ye
A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.
April 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29651023/generation-of-autochthonous-mouse-models-of-clear-cell-renal-cell-carcinoma-mouse-models-of-renal-cell-carcinoma
#18
REVIEW
Weibin Hou, Zhigang Ji
Renal cell carcinoma (RCC) is one of the 10 most common cancers worldwide, and to date, a strong systemic therapy has not been developed to treat RCC, even with the remarkable modern advances in molecular medicine mostly due to our incomplete understanding of its tumorigenesis. There is a dire unmet need to understand the etiology and progression of RCC, especially the most common subtype, clear cell RCC (ccRCC), and to develop new treatments for RCC. Genetically engineered mouse (GEM) models are able to mimic the initiation, progression, and metastasis of cancer, thus providing valuable insights into tumorigenesis and serving as perfect preclinical platforms for drug testing and biomarker discovery...
April 13, 2018: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29650944/glucose-related-protein-78-expression-and-its-effects-on-cisplatin-resistance-in-cervical-cancer
#19
Chengyan Luo, Wen Fan, Yi Jiang, Shulin Zhou, Wenjun Cheng
BACKGROUND GRP78, the 78-kDa glucose-regulated protein, occupies a significant position in endoplasmic reticulum stress. Emerging evidences have shown that GRP78 induces chemoresistance in several tumors; however, the role of GRP78 in cervical cancer (CVC) still needs to be elucidated clearly. MATERIAL AND METHODS In the present study, we determined the expression levels of GRP78 in CVC tissues collected from patients through immuocytochemistry, western blot and real-time PCR. To evaluate the exact role of GRP78 in CVC cells in the presence of cisplatin, we generated GRP78 knock-down cervical cancer cells through small interfering RNA...
April 13, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29650825/-successful-breast-conserving-surgery-for-simultaneous-and-ipsilateral-multiple-breast-carcinomas-of-young-patient-a-case-report
#20
Hitomi Kubota, Keita Adachi, Shuhei Suzuki, Shigeru Fujisaki, Yukiko Hara, Katsuhisa Enomoto, Ryouichi Tomita, Tomohiro Hirano, Kenichi Sakurai
We report a case of a simultaneous and ipsilateral multiple breast carcinoma.The patient was 35-year-old woman.She was noticed a breast lump on her right breast, and visited our hospital.Mammography showed a tumor accompanied by spiculation on her right breast.Ultrasonography revealed 2 tumors with irregular margins, 23mm in diameter and 12mm in diameter, and were observed on C area of her right breast.The continuity of the 2 tumors was not clear.Core needle biopsies for each breast tumors led to a diagnosis...
April 2018: Gan to Kagaku Ryoho. Cancer & Chemotherapy
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