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https://www.readbyqxmd.com/read/29044859/testing-feasibility-of-an-accurate-microscopic-assessment-of-macrovesicular-steatosis-in-liver-allograft-biopsies-by-smartphone-add-on-lenses
#1
Manuela Cesaretti, Nicolas Poté, Federica Dondero, François Cauchy, Anne Sophie Schneck, Olivier Soubrane, Valerie Paradis, Alberto Diaspro, Andrea Antonini
Light microscopy is an essential tool in histological examination of tissue samples. However, the required equipment for a correct and rapid diagnosis is sometimes unavailable. Smartphones and mobile phone networks are widespread, and could be used for diagnostic imaging and telemedicine. Macrovesicular steatosis (MS) is a major risk factor for liver graft failure, and is only assessable by microscopic examination of a frozen tissue section. The aim of this study was to compare the microscopic assessment of MS in liver allograft biopsies by a smartphone with eyepiece adaptor (BLIPS device) to standard light microscopy...
October 17, 2017: Microscopy Research and Technique
https://www.readbyqxmd.com/read/29044289/preoperative-diagnostics-in-differentiated-thyroid-carcinoma
#2
Philipp Seifert, Martin Freesmeyer
AIM: The preoperative diagnosis of differentiated thyroid carcinomas (DTC) is particularly challenging in view of the high prevalence of thyroid nodules in the general population. Incidental diagnosis of DTC, on the other hand, can lead to reoperations and increased risk of morbidity. This study aimed to assess the prediction value of preoperative DTC dignity assessment and it's impact on surgical approaches. METHODS: We retrospectively reviewed 107 cases of DTC and subdivided them in three groups based on the preoperative dignity assessment: suspected malignancy (SM), unclear dignity (UD), and incidental findings (IF)...
October 18, 2017: Nuklearmedizin. Nuclear Medicine
https://www.readbyqxmd.com/read/29034605/useful-aspects-of-diagnosis-of-imprint-cytology-in-intraoperative-consultation-of-ovarian-tumors-comparison-between-imprint-cytology-and-frozen-sections
#3
Shiho Azami, Yuuji Aoki, Mizuki Iino, Asumi Sakaguchi, Kanako Ogura, Daiki Ogishima, Toshiharu Matsumoto
BACKGROUND: In the intraoperative consultation of ovarian tumors, the histological diagnosis of frozen sections (FS) of large tumors is frequently difficult because of the limited number of tumor samples. The application of imprint cytology (IC), in which samples are obtained from wide areas of the tumors, is useful for intraoperative consultation. However, the useful aspects of IC have not been clearly defined. The present study is a detailed comparison of IC and FS that clearly defines the useful aspects of IC...
October 16, 2017: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/29034530/gene-expression-and-morphological-changes-in-the-intestinal-mucosa-associated-with-increased-permeability-induced-by-short-term-fasting-in-chickens
#4
S Gilani, G S Howarth, G Nattrass, S M Kitessa, R Barekatain, R E A Forder, C D Tran, R J Hughes
Short-term fasting for 4.5 and 9 hr has been demonstrated to increase intestinal permeability (IP) in chickens. This study aimed to investigate the effects of 0, 4.5, 9 and 19.5 hr fasting on intestinal gene expression and villus-crypt architecture of enterocytes in jejunal and ileal samples. On day 38, Ross-308 male birds were fasted according to their group and then euthanised. Two separate intestinal sections (each 2 cm long, jejunum and ileum) were collected. One section was utilised for villus height and crypt depth measurements...
October 16, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/29033402/5-aminolevulinic-acid-5-ala-fluorescence-guided-mohs-surgery-resection-of-penile-scrotal-extramammary-paget-s-disease
#5
Xiaoqiong Peng, Wei Qian, Jiangang Hou
This report aims to evaluate the usefulness of 5-aminolevulinic acid (5-ALA) fluorescence-guided Mohs surgery resection of penile-scrotal extramammary Paget's disease for achieving maximum tumor resection. Between January 2014 and December 2015, 5 patients underwent surgical resection of a penile-scrotal extramammary Paget's disease in department of urology, Huashan hospital, Fudan University. All patients were coated with 5-ALA (concentration of 20%) throughout the scrotum and the visible range of the lesion plus a 2cm margin 3 hours before the induction of anesthesia...
October 16, 2017: Bioscience Trends
https://www.readbyqxmd.com/read/29032883/evaluating-the-accuracy-of-intraoperative-frozen-section-during-inguinal-lymph-node-dissection-in-penile-cancer
#6
Juan Chipollini, Dominic H Tang, Neil Manimala, Scott M Gilbert, Julio M Pow-Sang, Wade J Sexton, Michael A Poch, Philippe E Spiess
INTRODUCTION: Inguinal lymph node dissection is an integral part in the management of invasive penile tumors with intraoperative assessment often aiding decision-making during dissection. In this study, we evaluate the diagnostic value of intraoperative frozen section (FS) and analyze clinicopathologic factors that affect its accuracy. MATERIAL AND METHODS: We, retrospectively, reviewed 84 patients with squamous cell carcinoma of the penis who underwent inguinal lymph node dissection at our institution...
October 9, 2017: Urologic Oncology
https://www.readbyqxmd.com/read/29027854/long-term-outcomes-of-tissue-based-acth-antibody-assay-guided-transsphenoidal-resection-of-pituitary-adenomas-in-cushing-disease
#7
J Mark Erfe, Avital Perry, John McClaskey, Silvio E Inzucchi, Whitney Sheen James, Tore Eid, Richard A Bronen, Amit Mahajan, Anita Huttner, Florecita Santos, Dennis Spencer
OBJECTIVE Cushing disease is caused by a pituitary micro- or macroadenoma that hypersecretes adrenocorticotropic hormone (ACTH), resulting in hypercortisolemia. For decades, transsphenoidal resection (TSR) has been an efficacious treatment but with certain limitations, namely precise tumor localization and complete excision. The authors evaluated the novel use of a double-antibody sandwich assay for the real-time quantitation of ACTH in resected pituitary specimens with the goals of augmenting pathological diagnosis and ultimately improving long-term patient outcome...
October 13, 2017: Journal of Neurosurgery
https://www.readbyqxmd.com/read/29026518/castleman-s-disease-a-rare-presentation-in-a-retroperitoneal-accessory-spleen-treated-with-a-minimally-invasive-robotic-approach
#8
Fabio Sbrana, Dimin Zhou, Ina Zamfirova, Nathaniel Leonardi
Unicentric Castleman's disease (UCD) is a rare disorder of unknown etiology characterized by localized lymphoid tissue proliferation and interfollicular hypervascularity. A 33-year-old Caucasian female presented with vague abdominal discomfort and pain with pressure. Ultrasound and computed tomography detected a large peripancreatic mass. Robotic-assisted resection of the mass along with en bloc dissection of the encased left adrenal gland was done. Frozen section examination confirmed UCD hyaline vascular variant in a retroperitoneal accessory spleen...
October 2017: Journal of Surgical Case Reports
https://www.readbyqxmd.com/read/29018641/prevalence-and-determinants-of-frozen-shoulder-in-patients-with-diabetes-a-single-center-experience-from-pakistan
#9
Faisal Inayat, Nouman Safdar Ali, Haroon Shahid, Fariha Younus
Introduction Frozen shoulder (FS) or adhesive capsulitis is a constellation of symptoms like pain, stiffness, and/or functional deficit at the glenohumeral joint. It is one of the musculoskeletal complications in patients with diabetes that can be particularly debilitating. The aim of this study is to estimate the prevalence of FS and to compare the determinants of this disease in a population with diabetes from Lahore, Pakistan. Materials and Methods We carried out this cross-sectional study on a systematically randomized sample of 80 patients with diabetes...
August 6, 2017: Curēus
https://www.readbyqxmd.com/read/29018181/perineural-infiltration-as-a-prognostic-factor-in-surgically-treated-gallbladder-cancer-a-single-center-experience-and-literature-review
#10
Claudio Francesco Feo, Maria Laura Cossu, Giorgio Carlo Ginesu, Alessandro Fancellu, Antonio Mario Scanu, Nadia Piras, Giuseppe Cherchi, Antonio Cossu, Giovanni Sotgiu, Marco Pazzona, Alberto Porcu
INTRODUCTION: Gallbladder cancer (GBC) is the most incident cancer of the biliary tract with only 5-13% of the sufferers surviving for five years. The aim of this study was to evaluate the prognostic role of perineural invasion (PNI) and its association with several clinicopathological variables in a cohort of surgically treated patients, and through a comprehensive review of the scientific literature. MATERIALS AND METHODS: Twenty-five consecutive patients submitted to curative surgery for GBC from 2008 through 2016 were enrolled...
October 4, 2017: Annali Italiani di Chirurgia
https://www.readbyqxmd.com/read/29016707/minimal-clinical-impact-of-intraoperative-examination-of-sentinel-lymph-nodes-in-patients-with-ductal-carcinoma-in-situ-an-opportunity-for-improved-resource-utilization
#11
Eugene T Shin, Amy S Joehlin-Price, Doreen M Agnese, Debra L Zynger
Objectives: There is little information regarding sentinel lymph node (SLN) frozen-section examination in patients with a history of ductal carcinoma in situ (DCIS). We evaluated the usage, clinical impact, and pathology resources used for SLN cryosectioning in mastectomy cases with a DCIS history. Methods: Mastectomies with SLNs submitted from 2012 to 2013 at a tertiary care center were analyzed. Medicare reimbursement was used to estimate pathology health care expenditures of intraoperative frozen sections...
September 18, 2017: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/29016546/histologic-accuracy-of-mohs-micrographic-surgery
#12
James T Highsmith, M Jason Highsmith, Gary D Monheit
BACKGROUND: Mohs micrographic surgery enables the surgeon to maximize tumor removal while minimizing the amount of tissue being removed using advanced mapping techniques combined with microscopy. Interpretation of frozen section slides is vital to the success of Mohs micrographic surgery. OBJECTIVE: Evaluate and confirm the congruence of frozen section slide interpretation between fellowship-trained Mohs surgeons and dermatopathologists. METHODS: Retrospective cohort concordance study spanning 10 years (n = 1,720 cases)...
October 6, 2017: Dermatologic Surgery: Official Publication for American Society for Dermatologic Surgery [et Al.]
https://www.readbyqxmd.com/read/28992289/transcriptomics-in-kidney-biopsy-is-an-untapped-resource-for-precision-therapy-in-nephrology-a-systematic-review
#13
Francesco Paolo Schena, Ionut Nistor, Claudia Curci
Background: The diagnosis of glomerular diseases is based on the evaluation of histological lesions in renal tissue by means of light and electronic microscopy, and immunofluorescence technique. Frozen and archival formalin-fixed paraffin-embedded kidney biopsies represent a stored resource for high-throughput technologies. Transcriptomics makes it possible to study the whole gene-expression profile of cells and tissues in a specific period and/or condition. The results, whether considered alone or integrated with other omics data, could help to improve existing knowledge about the pathogenetic mechanisms of glomerulopathies...
August 1, 2017: Nephrology, Dialysis, Transplantation
https://www.readbyqxmd.com/read/28990674/the-futility-of-intraoperative-frozen-section-in-the-evaluation-of-follicular-thyroid-lesions
#14
Craig A Bollig, David Lesko, David Gilley, Laura M Dooley
OBJECTIVE: Investigate the utility of intraoperative frozen section (iFS) in patients with follicular thyroid lesions following publication of the 2015 American Thyroid Association (ATA) guidelines. STUDY DESIGN: Retrospective chart review. METHODS: Patient demographics, preoperative cytology, frozen pathology, and final pathology were reviewed on patients undergoing thyroid surgery at a tertiary care hospital in which iFS was utilized over a 5-year period...
October 9, 2017: Laryngoscope
https://www.readbyqxmd.com/read/28988423/positive-fresh-frozen-section-margins-as-an-adverse-independent-prognostic-factor-for-local-recurrence-in-oral-cancer-patients
#15
Mateusz Szewczyk, Wojciech Golusinski, Jakub Pazdrowski, Michal Masternak, Neil Sharma, Pawel Golusinski
OBJECTIVES: To access 1) the value of further surgical resection (completion surgery) in cases with a positive intraoperative margin analysis, and 2) whether cancers that undergo completion surgery following positive intraoperative margin analysis with subsequent negative margins should be considered true margin-negative (R0) resections in terms of adjuvant treatment planning. STUDY DESIGN: Retrospective analysis of patients with primary oral cancer. METHODS: One hundred and fifty-one patients underwent primary surgical resection of oral squamous cell carcinoma with intraoperative margin examination...
October 8, 2017: Laryngoscope
https://www.readbyqxmd.com/read/28988021/two-concurrent-appendiceal-neoplasms-in-an-elderly-patient-a-case-report
#16
Riva Das, Joshua P Cantor, Thai Q Vu
INTRODUCTION: Acute appendicitis, one of the commonest surgical diagnoses, is rare and more complex presentation in the elderly. Physicians must consider atypical causes appendicitis in this population, which could affect the management of the patient. PRESENTATION OF CASE: An elderly female presented with a two-day history of lower abdominal pain, associated with low-grade fevers and chills. Studies showed leukocytosis and computed tomography (CT) findings consistent with appendicitis...
September 28, 2017: International Journal of Surgery Case Reports
https://www.readbyqxmd.com/read/28986909/laser-capture-microdissection-of-maize-kernel-compartments-for-rna-seq-based-expression-analysis
#17
Shanshan Zhang, Dhiraj Thakare, Ramin Yadegari
Laser-capture microdissection (LCM) enables isolation of single cells or groups of cells for a variety of downstream applications including transcriptome profiling. Recently, this methodology has found a more widespread use particularly with the advent of next-generation sequencing techniques that enable deep profiling of the limited amounts of RNA obtained from fixed or frozen sections. When used with fixed tissues, a major experimental challenge is to balance the tissue integrity needed for microscopic visualization of the cell types of interest with that of the RNA quality necessary for deep profiling...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28985593/effects-of-chilled-then-frozen-storage-up-to-52weeks-on-an-indicator-of-protein-oxidation-and-indices-of-protein-degradation-in-lamb-m-longissimus-lumborum
#18
Cassius E O Coombs, Benjamin W B Holman, Damian Collins, Matthew J Kerr, Michael A Friend, David L Hopkins
This study investigated the protein oxidation properties of lamb following chilled-then-frozen storage. Experimental (n=360) M. longissimus lumborum (LL) were randomly sampled from the boning room of a commercial Australian abattoir, at 24h post mortem, and assigned to five chilled storage periods (0, 2, 4, 6 and 8weeks) and six subsequent frozen storage periods (0, 4, 8, 12, 24 and 52weeks). Upon completion of each storage treatment combination, corresponding LL were sub-sectioned and analysed for carbonyl content, protein solubility, nitrate/nitrite content, particle size analysis and estimated myoglobin fractions...
September 28, 2017: Meat Science
https://www.readbyqxmd.com/read/28985486/the-effect-of-ankle-distraction-on-arthroscopic-evaluation-of-syndesmotic-instability-a-cadaveric-study
#19
Bart Lubberts, Daniel Guss, Bryan G Vopat, Jonathon C Wolf, Daniel K Moon, Christopher W DiGiovanni
BACKGROUND: To assist with visualization, orthopaedic surgeons often apply ankle distraction during arthroscopic procedures. The study aimed to investigate whether ankle distraction suppresses fibular motion in cadaveric specimens with an unstable syndesmotic injury. METHODS: Fourteen fresh-frozen above knee specimens underwent arthroscopic assessment with 1) intact ligaments, 2) after sectioning of the anterior inferior tibiofibular ligament, the interosseous ligament, and the posterior inferior tibiofibular ligament, and 3) after sectioning of the deep and superficial deltoid ligament...
September 28, 2017: Clinical Biomechanics
https://www.readbyqxmd.com/read/28984773/ovarian-torsion-of-mixed-epithelial-tumor-misdiagnosed-as-a-malignancy-in-postmenopausal-woman-a-case-report
#20
Hyeji Jeon, Aeli Ryu, Hui-Gyeong Seo, Si-Hyong Jang
RATIONALE: Adnexal torsion is 1 of the most common emergency gynecological disease. It is more often diagnosed in reproductive age, but rarely in postmenopausal women. The clinical symptoms of adnexal torsion are nonspecific in postmenopausal women. Epithelial ovarian tumors are common in adults, and the risk of malignancy increases with age, especially after menopause. So, it is difficult to diagnose adnexal torsion precisely compared with reproductive women, and most cases of adnexal torsion with postmenopausal women are diagnosed as a malignancy preoperatively...
October 2017: Medicine (Baltimore)
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