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Frozen sections

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https://www.readbyqxmd.com/read/29228314/demodifying-rna-for-transcriptomic-analyses-of-archival-formalin-fixed-paraffin-embedded-samples
#1
Leah C Wehmas, Charles E Wood, Remi Gagne, Andrew Williams, Carole Yauk, Mark M Gosink, Deidre Dalmas, Ruixin Hao, Raegan O'Lone, Susan Hester
Archival formalin-fixed paraffin-embedded (FFPE) tissue samples offer a vast but largely untapped resource for genomic research. The primary technical issues limiting use of FFPE samples are RNA yield and quality. In this study, we evaluated methods to demodify RNA highly fragmented and crosslinked by formalin fixation. Primary endpoints were RNA recovery, RNA-sequencing quality metrics, and transcriptional responses to a reference chemical (phenobarbital, PB). Frozen mouse liver samples from control and PB groups (n = 6/group) were divided and preserved for 3 months as follows: frozen (FR); 70% ethanol (OH); 10% buffered formalin for 18 hours followed by ethanol (18F); or 10% buffered formalin (3F)...
December 7, 2017: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/29227576/microscopic-investigation-of-topically-applied-nanoparticles-for-molecular-imaging-of-fresh-tissue-surfaces
#2
Soyoung Kang, Yu Winston Wang, Xiaochun Xu, Eric Navarro, Kenneth M Tichauer, Jonathan T C Liu
Previous studies have shown that functionalized nanoparticles (NPs) topically applied on fresh tissues are able to rapidly target cell-surface protein biomarkers of cancer. Furthermore, studies have shown that a paired-agent approach, in which an untargeted NP is co-administered with a panel of targeted NPs, controls for nonspecific behavior of the NPs, enabling quantitative imaging of biomarker expression. However, given the complexities in nonspecific accumulation, diffusion, and chemical binding of targeted NPs in tissues, studies are needed to better understand these processes at the microscopic scale...
December 11, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/29227106/hollow-silica-nanoparticles-penetrate-the-peripheral-nerve-and-enhance-the-nerve-blockade-from-tetrodotoxin
#3
Qian Liu, Claudia M Santamaria, Tuo Wei, Chao Zhao, Tianjiao Ji, Tianshe Yang, Andre Shomorony, Bruce Y Wang, Daniel S Kohane
The efficacy of tetrodotoxin (TTX), a very potent local anesthetic, is limited by its poor penetration through barriers to axonal surfaces. To address this issue, we encapsulated TTX in hollow silica nanoparticles (TTX-HSN) and injected them at the sciatic nerve in rats. TTX-HSN achieved an increased frequency of successful blocks, prolonged the duration of the block, and decreased the toxicity compared to free TTX. In animals injected with fluorescently labeled HSN, the imaging of frozen sections of nerve demonstrated that HSN could penetrate into nerve and that the penetrating ability of silica nanoparticles was highly size-dependent...
December 11, 2017: Nano Letters
https://www.readbyqxmd.com/read/29221312/port-access-thoracoscopic-bisubsegmentectomy-of-right-upper-lobe-posterior-and-anterior-segments
#4
Hirohisa Kato, Hiroyuki Oizumi, Jun Suzuki, Akira Hamada, Hikaru Watarai, Kenta Nakahashi, Mitsuaki Sadahiro
A 64-year-old woman was admitted to our hospital with a 16-mm non-solid tumor with pure ground-glass nodule (GGN) contents in the posterior segment near the anterior segment of her right upper lung lobe that was suspicious of adenocarcinoma in situ (AIS). Three-dimensional computed tomography (3DCT) simulation was performed to identify the subsegmental artery and vein pre- or intra-operatively. Port-access thoracoscopic bisubsegmentectomy of the right upper lobe was performed. A frozen section revealed AIS...
September 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/29221311/uniportal-video-assisted-thoracic-surgery-lobectomy-in-a-patient-with-carcinoid-tumor-located-at-the-orifice-of-the-right-lower-lobe
#5
Semih Halezeroğlu
We present here a 47-year old male patient who had a typical carcinoid tumor located at the orifice of right lower lobe bronchus underwent uniportal thoracoscopic lower lobectomy following bronchoscopic removal of the endobronchial tumor. Tumor was seen by bronchoscopy to protrude out from the lower lobe into the intermediary bronchus obstructing the lower lobe completely and the middle lob partially. However, the origin of the endobronchial tumor was in the distal part of the lower lobe bronchus. In this case, open surgery could be an alternative to save the middle lobe by incising the lower lobe bronchus to pull out the endobronchial tumor first during thoracotomy...
September 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/29221308/how-to-manage-tumor-located-between-upper-division-and-lingular-segment-s3-s4-segmentectomy-and-s3b-s4-segmentectomy
#6
Shin-Ichi Yamashita, Yasuhiro Yoshida, Daisuke Hamatake, Takeshi Shiraishi, Katsunobu Kawahara, Akinori Iwasaki
Segmentectomy is one of the treatment of choice for small-sized non-small cell lung cancer (NSCLC). Although simple segmentectomy is feasible even if under thoracoscopy, complicated segmentectomy which contains more than two segmental plane divisions is difficult especially thoracoscopic surgery. We here present the case of totally thoracoscopic segmentectomy between upper division and lingular segment. In the first case, the 64-year-old female patient admitted for further examination and treatment of left lung ground glass nodule...
September 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/29221304/microinvasive-segmentectomy-in-a-sculpting-manner-a-case-of-vats-left-s1-2-segmentectomy
#7
Liang Xue, Yunfeng Yuan
A ground-glass opacity (GGO) lesion was discovered in a 64-year-old female 1 year ago. One month before administration, a follow-up CT showed the lesion in the apical segment of left upper lobe had increased from 8 to 11 mm in diameter. The lesion was diagnosed to be cT1aN0M0 non-small cell lung cancer (NSCLC) and a 3-port video-assisted thoracic surgery (VATS) anatomic segmentectomy was performed. Intraoperative frozen sections revealed a microinvasive adenocarcinoma. Systematic lymph node dissection was then carried out...
September 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/29218663/laparoscopic-extended-cholecystectomy-for-t3-gallbladder-cancer
#8
Sungho Kim, Yoo-Seok Yoon, Ho-Seong Han, Jai Young Cho, YoungRok Choi
BACKGROUND: Gallbladder cancer (GBC) has been contraindicated for laparoscopic surgery since this procedure was introduced [1, 2]. Recently, however, there have been several reports of laparoscopic extended cholecystectomy for GBC, but most cases involved early GBC confined to the GB [3, 4]. This video describes our technique of laparoscopic extended cholecystectomy for T3 GBC. METHODS: A 77-year-old female presented with a gallbladder mass, which was incidentally detected during evaluation of back pain...
December 7, 2017: Surgical Endoscopy
https://www.readbyqxmd.com/read/29218614/18f-fluorodeoxyglucose-specimen-positron-emission-mammography-delineates-tumour-extension-in-breast-conserving-surgery-preliminary-results
#9
Gou Watanabe, M Itoh, X Duan, H Watabe, N Mori, H Tada, A Suzuki, M Miyashita, N Ohuchi, T Ishida
OBJECTIVES: We aimed to determine whether high-resolution specimen-positron emission mammography (PEM) using fluorodeoxyglucose (18F-FDG) can reveal extension of breast cancer in breast-conserving surgery (BCS), and assess the safety of radiation exposure to medical staff. METHODS: Sixteen patients underwent positron emission tomography, and then BCS with intraoperative frozen section analysis on the same day. Resected specimens with remaining 18F-FDG accumulation were scanned by high-resolution PEM...
December 7, 2017: European Radiology
https://www.readbyqxmd.com/read/29214370/lumbar-intervertebral-disc-allograft-transplantation-the-revascularisation-pattern
#10
Yong-Can Huang, Jun Xiao, Victor Y Leung, William W Lu, Yong Hu, Keith D K Luk
PURPOSE: Fresh frozen intervertebral disc allograft transplantation has been reported to be a viable treatment option for advanced degenerative disc diseases, but rapid degeneration of the postoperative allograft was found. Loss of nutrient supply is believed to be the most likely inducer because the disc allografts have to endure in an ischaemic environment until the nutrient pathway is re-established. The aim of this study was to focus on the revascularisation of the disc allograft after transplantation in goats...
December 6, 2017: European Spine Journal
https://www.readbyqxmd.com/read/29204102/diagnostic-accuracy-of-touch-imprint-cytology-for-head-and-neck-malignancies-a-useful-intra-operative-tool-in-resource-limited-countries
#11
Hania Naveed, Mariam Abid, Atif Ali Hashmi, Muhammad Muzammamil Edhi, Ahmareen Khalid Sheikh, Ghazala Mudassir, Amir Khan
Background: Intraoperative consultation is an important tool for the evaluation of the upper aerodigestive tract (UAT) malignancies. Although frozen section analysis is a preferred method of intra-operative consultation, however in resource limited countries like Pakistan, this facility is not available in most institutes; therefore, we aimed to evaluate the diagnostic accuracy of touch imprint cytology for UAT malignancies using histopathology of the same tissue as gold standard. Methods: The study involved 70 cases of UAT lesions operated during the study period...
2017: BMC Clinical Pathology
https://www.readbyqxmd.com/read/29201446/surgical-treatment-of-low-and-intermediate-grade-lung-net
#12
Mariano García-Yuste, José María Matilla, Miguel Angel Cañizares, Laureano Molins, Ricardo Guijarro
Background: Carcinoids now constitute complex tumours which require a multidisciplinary approach and long-term follow-up. Surgical intervention is nowadays confirmed as the mainstay of treatment. Methods: From 1980 to 2015, EMETNE-SEPAR collected 1,339 patients treated surgically for bronchial carcinoid (1,154 typical and 185 atypical carcinoids). Standard and conservative procedures were considered with regard to surgical approach. All the patients with carcinoid were pathologically coded following the standards of the 7th edition 2009 TNM lung cancer staging...
November 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/29201322/the-diagnostic-value-of-fine-needle-aspiration-in-comparison-with-frozen-section-in-thyroid-nodules-a-20-year-study
#13
Sepideh Siadati, Seyed Mozafar Rabiee, Ebrahim Alijanpour, Mohammad Ali Bayani, Novin Nikbakhsh
Background: Fine needle aspiration (FNA) is the most important method in the diagnosis of thyroid nodules before surgery. Recently, the efficiency of FNA in thyroid nodule management has been debatable. On the other hand, intraoperative frozen section (FS) has been used to confirm the diagnosis of FNA and select the proper surgical approach. In this regard, the present study aimed to assess the diagnostic value of FNA as compared to FS in the diagnosis of thyroid nodules. Methods: This retrospective study was performed on 69 patients with FNA and FS and histopathological examination from 1993 to 2014 in Babol, northern Iran...
2017: Caspian Journal of Internal Medicine
https://www.readbyqxmd.com/read/29200703/rhabdomyosarcoma-of-the-iliopsoas-a-retroperitoneal-misdiagnosis
#14
Animesh Ashutosh Upadhyay
Rhabdomyosarcoma (RMS) is a rare soft tissue sarcoma. The already documented data regarding RMS state that it is more prevalent in males than females and also that its occurrence is more in Caucasians than Asians. The current incidence of RMS is 4.5 cases/million, and thus, it is a very rare cancer. The undifferentiated type is the most aggressive one with a rare presentation in the retroperitoneum. Overall, this case emphasizes that consideration should be given to wide range of diagnosis and that frozen section is the gold standard for a confirmatory diagnosis, as the first biopsy showed benign cells within the tumor...
July 2017: Indian Journal of Medical and Paediatric Oncology
https://www.readbyqxmd.com/read/29198817/whole-specimen-intraoperative-frozen-section-analysis-experience-with-1082-basal-cell-carcinomas
#15
Muhammed A Kedilioglu, Paul G Bos, Kim De Jong, Niels A Noordzij, Robby E Kibbelaar, Oren Lapid, Chantal M Mouës
BACKGROUND: Basal cell carcinomas (BCCs) excised leaving positive tumour margins, are at a higher risk of recurrence. Accordingly, complete tumour removal with preservation of healthy tissue, aiming for low recurrence rates, is the main goal in treating BCCs. OBJECTIVE: The present study aimed to identify the reliability of the Whole Specimen Intraoperative Frozen Section Analysis (WIFSA) technique by comparing intraoperative WIFSA and postoperative Formalin-Fixed Paraffin-Embedded section analysis (FFPE) results in 1082 basal cell carcinomas and by assessing the recurrence rates during a follow-up period up to 10 years...
November 23, 2017: European Journal of Surgical Oncology
https://www.readbyqxmd.com/read/29198654/evaluation-of-frozen-and-paraffin-sections-using-the-maryland-aggregate-pathology-index-score-in-donor-kidney-biopsy-specimens-of-a-brazilian-cohort
#16
A C Teixeira, C C Freire de Carvalho, G P Mororó, L D M Pereira, V S Lacerda, R M Esmeraldo
The Maryland aggregate pathology index (MAPI) score is an important tool developed to help pathologists in evaluating potential organs for transplantation. It is based on analysis of five variables (present or absent): arteriolar hyalinosis (4 points), periglomerular fibrosis (2 points), arterial wall-lumen ratio superior to 0.5 (2 points), global glomerulosclerosis in more than 15% of glomeruli (2 points), and interstitial scar (3 points). Kidneys with a MAPI score >7 points are considered inadequate for donation...
December 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/29196067/margin-assessment-in-renal-surgery-using-a-handheld-optical-coherence-tomography-probe
#17
Wesley W Ludwig, Sara E Wobker, Mark W Ball, Adam M Zysk, Kiran S Yemul, Philip M Pierorazio, Michael A Gorin, Mohamad E Allaf
OBJECTIVE: To assess the use of a handheld optical coherence tomography (OCT) probe for the evaluation of intraoperative surgical margins during partial nephrectomy (PN). METHODS: In an initial feasibility study, a radical nephrectomy specimen with a 9-cm tumor was cut into 19 sections, exposing 0 mm (n=8), 1 mm (n=6), and 2 mm (n=5) gross margins. OCT was used to determine the margin width in each specimen. Second, a prospective ex vivo assessment of 15 PN tumor specimens was performed with OCT to determine margin status and to measure the attenuation coefficient (AC) of tumor and renal parenchyma...
November 28, 2017: Urology
https://www.readbyqxmd.com/read/29194190/an-evaluation-of-frozen-section-and-lymph-node-dissection-results-for-mucinous-ovarian-tumors
#18
Marisa R Moroney, Miriam D Post, Amber A Berning, Jeanelle Sheeder, Bradley R Corr
OBJECTIVES: Intraoperative frozen section has greater than 90% accuracy for ovarian tumors; however, mucinous histology has been shown to be associated with increased frozen section inaccuracy. Recent data demonstrate that primary ovarian mucinous carcinomas have no lymph node involvement, even when extraovarian disease is present, and therefore may not require lymph node dissection. Our primary objective is to evaluate the accuracy of identifying mucinous histology on frozen section...
November 30, 2017: International Journal of Gynecological Cancer
https://www.readbyqxmd.com/read/29191199/paraffin-immunofluorescence-for-detection-of-immune-complexes-in-renal-biopsies-an-efficient-salvage-technique-for-diagnosis-of-glomerulonephritis-in-dogs
#19
Akira Yabuki, Mariko Sawa, Moeko Kohyama, Takeshi Hamamoto, Osamu Yamato
BACKGROUND: Renal biopsy is an essential tool for the diagnosis of proteinuric kidney diseases in dogs, and evaluation of immune complexes (IC) by immunofluorescence (IF) of frozen sections (IF-F) is required for the diagnosis of IC-mediated glomerulonephritis (ICGN). However, the use of frozen sections from renal biopsies can have limitations. The aim of this study was to develop a reliable IF method using formalin-fixed and paraffin-embedded (FFPE) sections to detect ICs in dog ICGN...
December 1, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/29188057/subungual-onychomycosis-due-to-aspergillus-niger-mimicking-a-glomus-tumor-a-case-report
#20
Yumi Matsuyama, Tomoki Nakamura, Tomohito Hagi, Kunihiro Asanuma, Akihiro Sudo
Onychomycosis is a common nail infection caused by dermatophytes, while non-dermatophytes including Aspergillus spp. are causes of nail onychomycosis. Aspergillus niger is not common as a cause of nail onychomycosis. In the current study we present a 60-year-old woman with subungual onychomycosis due to Aspergillus niger mimicking a glomus tumor. Physical examination revealed right thumb had a black color of nail bed. Localized tenderness and severe pain were observed. However, the cold sensitivity test, Loves pin test and Hildreths test were negative...
December 2017: Biomedical Reports
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