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https://www.readbyqxmd.com/read/29694605/neuroprotective-effects-of-erythropoietin-on-rat-retinas-subjected-to-oligemia
#1
Litia Alves de Carvalho, Renata Fleming, Moysés Sant'Anna, Roberta Guimarães, Adalmir Morterá Dantas, Eduardo Morizot-Leite, Leny A Cavalcante, Silvana Allodi
OBJECTIVES: Erythropoietin may have neuroprotective potential after ischemia of the central nervous system. Here, we conducted a study to characterize the protective effects of erythropoietin on retinal ganglion cells and gliotic reactions in an experimentally induced oligemia model. METHODS: Rats were subjected to global oligemia by bilateral common carotid artery occlusion and then received either vehicle or erythropoietin via intravitreal injection after 48 h; they were euthanized one week after the injection...
2018: Clinics
https://www.readbyqxmd.com/read/29692237/ping-pong-ball-tumor-or-thrombus-in-the-right-atrium-a-case-report
#2
Stephanie Ghaleb, Bryant Roosevelt, James Cnota
Tumors and thrombi are the most common cardiac masses of the right atrium. The use of noninvasive imaging to differentiate between the two can be deceiving, and the clinical judgment of a cardiologist and the emergency of the situation should be partnered to decide on the next step of the management. We present the case of a 29-year-old lady who was receiving neoadjuvant treatment for her rhabdomyosarcoma and was incidentally found to have a very large, very mobile right atrial mass that was protruding in the right ventricle with each cardiac cycle along with findings of a small segmental right lower lobe pulmonary embolism...
May 2018: World Journal for Pediatric & Congenital Heart Surgery
https://www.readbyqxmd.com/read/29691646/retrospective-validation-of-the-laparoscopic-icg-sln-mapping-in-patients-with-grade-3-endometrial-cancer
#3
Andrea Papadia, Maria Luisa Gasparri, Anda P Radan, Chantal A L Stämpfli, Tilman T Rau, Michael D Mueller
PURPOSE: To evaluate the sensitivity, negative predictive value (NPV) and false-negative (FN) rate of the near infrared (NIR) indocyanine green (ICG) sentinel lymph node (SLN) mapping in patients with poorly differentiated endometrial cancer who have undergone a full pelvic and para-aortic lymphadenectomy after SLN mapping. METHODS: We performed a retrospective analysis of patients with endometrial cancer undergoing a laparoscopic NIR-ICG SLN mapping followed by a systematic pelvic and para-aortic lymphadenectomy...
April 24, 2018: Journal of Cancer Research and Clinical Oncology
https://www.readbyqxmd.com/read/29691554/-retrospective-analysis-of-896-cases-of-parotid-gland-tumor
#4
Zhi-Guo Zhao, Dan Gao, Jin Wang, Li-Ping Zhang
PURPOSE: To review the clinical data of patients undergoing operation, in order to summarize the incidence, proportion, clinical examination and diagnostic methods of parotid tumors. METHODS: Eight hundred and ninty-six cases of parotid gland tumors were collected from 2008 January to 2015 July from department of oral and maxillofacial surgery in Shengjing Hospital of China Medical University. A retrospective study of the clinical data was carried out, including age, gender, tumor location, diagnostic methods and pathological results...
December 2017: Shanghai Kou Qiang Yi Xue, Shanghai Journal of Stomatology
https://www.readbyqxmd.com/read/29690733/-experimental-study-on-the-effect-of-hepcidin-overexpression-on-osteoclasts-and-bone-mass-in-iron-accumulation-mice
#5
H Zhang, F Yang, A F Wang, P Jia, X Wang, Y Yuan, P Zhang, Y J Xu
Objective: To investigate the effects of hepcidin on iron, osteoclasts and bone mass in iron accumulation mice. Methods: Eight-week old C57/BL6 male mice were divided into control group (CTR group), high iron group (Fe group) and iron reduction group (Hepc group). CTR group and Fe group were wild-type mice, Hepc group were hepcidin gene overexpression mice.Fe group and Hepc group were injected intraperitoneally with iron, and the mice in the CTR group were injected intraperitoneally with the same amount of saline...
April 17, 2018: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/29688510/study-protocol-early-stereotactic-gamma-knife-radiosurgery-to-residual-tumor-after-surgery-of-newly-diagnosed-glioblastoma-gamma-gbm
#6
Stefanie Brehmer, Mario Alexander Grimm, Alex Förster, Marcel Seiz-Rosenhagen, Grit Welzel, Florian Stieler, Frederik Wenz, Christoph Groden, Sabine Mai, Daniel Hänggi, Frank Anton Giordano
BACKGROUND: Glioblastoma (GBM) is the most common malignant brain tumor in adult patients. Tumor recurrence commonly occurs around the resection cavity, especially after subtotal resection (STR). Consequently, the extent of resection correlates with overall survival (OS), suggesting that depletion of postoperative tumor remnants will improve outcome. OBJECTIVE: To assess safety and efficacy of adding stereotactic radiosurgery (SRS) to the standard treatment of GBM in patients with postoperative residual tumor...
April 24, 2018: Neurosurgery
https://www.readbyqxmd.com/read/29683930/importance-of-18f-fdg-pet-ct-to-select-patients-with-nonresectable-colorectal-liver-metastases-for-liver-transplantation
#7
Harald Grut, Mona-Elisabeth Revheim, Pål-Dag Line, Svein Dueland
OBJECTIVE: The aim of this study was to evaluate fluorine-18-fluorodeoxyglucose (F-FDG) PET/CT for the selection of patients with nonresectable colorectal liver metastases (NCLM) for liver transplantation (LT). In the secondary cancer study, we reported an improved 5-year overall survival in patients treated with LT for NCLM (56%) compared with chemotherapy (9%). However, many patients were rejected for LT owing to the detection of extrahepatic disease at preoperative imaging. PATIENTS AND METHODS: F-FDG PET/CT and contrast-enhanced computed tomography (ceCT) examinations before tentative LT for NCLM were assessed, and findings contraindicating LT were registered...
April 20, 2018: Nuclear Medicine Communications
https://www.readbyqxmd.com/read/29682361/recurrent-ameloblastoma-a-surgical-challenge
#8
Chithra Aramanadka, Abhay Taranath Kamath, Adarsh Kudva
Ameloblastoma is locally aggressive benign odontogenic tumour with increased risk of recurrence rate. The choice of treatment depends on the histologic subtype. Radical therapy is the recommended modality for solid ameloblastomas. The possibilities of recurrence even after enbloc resection are still high. The author presents two case reports of recurrent ameloblastomas postradical resection. First case describes the recurrence of ameloblastoma in the bone graft which was used for reconstruction, and the second case depicts recurrence in the infratemporal fossa...
2018: Case Reports in Dentistry
https://www.readbyqxmd.com/read/29681553/the-radicalization-of-breast-cancer-surgery-joseph-colt-bloodgood-s-role-in-william-stewart-halsted-s-legacy
#9
James R Wright
Johns Hopkins's surgeon William Stewart Halsted is renowned for popularizing the radical mastectomy, a disfiguring procedure that was overutilized during the 1900s. Cancer historians have questioned why Halsted, a meticulous surgical investigator, became more aggressive in his approach to breast cancer surgery when his own data failed to show prolonged patient survival. Joseph Colt Bloodgood, one of Halsted's early surgical residents, Hopkins's head of surgical pathology, and Halsted's primary outcome data analyst, played previously unrecognized roles...
2018: Bulletin of the History of Medicine
https://www.readbyqxmd.com/read/29680231/accuracy-of-intraoperative-frozen-section-diagnosis-of-borderline-ovarian-tumors-by-hospital-type
#10
Jaimin Shah, Michael Mackelvie, David M Gershenson, Preetha Ramalingam, Marylee M Kott, Jubilee Brown, Polly Gauthier, Elizabeth Nugent, Lois M Ramondetta, M Frumovitz
STUDY OBJECTIVE: To compare the accuracy of frozen section diagnosis of borderline ovarian tumors among three distinct types of hospital-academic hospital with gynecologic pathologists, academic hospital with non-gynecologic pathologists, and community hospital with non-gynecologic pathologists-and to determine if surgical staging alters patient care or outcomes for women with a frozen section diagnosis of borderline ovarian tumor. DESIGN: Retrospective study DESIGN CLASSIFICATION: Level II1 SETTING: Tertiary care, academic, and community hospitals PATIENTS: Women with an intraoperative frozen section diagnosis of borderline ovarian tumor at one of the aforementioned three types of hospital from April 1998 through June 2016...
April 19, 2018: Journal of Minimally Invasive Gynecology
https://www.readbyqxmd.com/read/29675374/comparison-of-three-fluorescence-labeling-and-tracking-methods-of-endothelial-progenitor-cells-in-laser-injured-retina
#11
Hui Shi, Xin-Rui Wang, Ming-Chao Bi, Wei Yang, Dan Wang, Hai-Le Liu, Ling-Ling Liang, Xiao-Hong Li, Qian Hao, Zhi-Hua Cui, E Song
AIM: To compare three kinds of fluorescent probes for in vitro labeling and in vivo tracking of endothelial progenitor cells (EPCs) in a mouse model of laser-induced retinal injury. METHODS: EPCs were isolated from human umbilical cord blood mononuclear cells and labeled with three different fluorescent probes: 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester (CFSE), 1,1'-dilinoleyl-3,3,3',3'-tetramethylindo-carbocyanine perchlorate linked acetylated low-density lipoprotein (DiI-AcLDL), and green fluorescent protein (GFP)...
2018: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/29673450/-establishment-of-multiple-indirect-immunofluorescence-technique-for-detecting-multiple-markers-in-the-same-specimens-of-hepatoma-cells-and-frozen-tissues
#12
Min Yang, Zhihua Tian, Bo Ma, Kaiying Jia, Hongli Zhang, Jing Fu, Wenwen Qin, Wei Zhao
Objective To establish a multiple-indirect immunofluorescence staining that can be used to detect several proteins in the same frozen section of hepatocellular carcinoma (HCC) tissues. Methods Laser scanning confocal microscopy (LSCM) was applied to scan the first group of antibodies against four proteins stained on the HCC tissues by the indirect immunofluorescence technique. The first group of antibodies and residual dye were removed by homologous species IgG Fab antibody blocking combined with chemical strip reagents (2-mercaptoethanol/dodecyl benzene sulfonic acid sodium/guanidine hydrochloride/sodium borohydride, β-ME/SDS/GnHCl/NaBH4)...
February 2018: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/29670830/improved-high-frequency-ultrasound-corneal-biometric-accuracy-by-micrometer-resolution-acoustic-property-maps-of-the-cornea
#13
Daniel Rohrbach, Ronald H Silverman, Dan Chun, Harriet O Lloyd, Raksha Urs, Jonathan Mamou
Purpose: Mapping of epithelial thickness (ET) is useful for detection of keratoconus, a disease characterized by corneal thinning and bulging in which epithelial thinning occurs over the apex. In prior clinical studies, optical coherence tomography (OCT) measurements of ET were systematically thinner than those obtained by 40-MHz high-frequency ultrasound (HFU) where a constant speed of sound ( c ) of 1636 m/s was used for all corneal layers. The purpose of this work was to study the acoustic properties, that is, c , acoustic impedance ( Z ), and attenuation ( α ) of the corneal epithelium and stroma independently using a scanning acoustic microscope (SAM) to investigate the discrepancy between OCT and HFU estimates of ET...
April 2018: Translational Vision Science & Technology
https://www.readbyqxmd.com/read/29668267/quantitation-of-endogenous-metabolites-in-mouse-tumors-using-mass-spectrometry-imaging
#14
John G Swales, Alex Dexter, Gregory Hamm, Anna Nilsson, Nicole Strittmatter, Filippos Michopoulos, Christopher Hardy, Pablo Morentin-Gutierrez, Martine Mellor, Per E Andren, Malcolm R Clench, Josephine Bunch, Susan E Critchlow, Richard J A Goodwin
Described is quantitative mass spectrometry imaging (qMSI) methodology for the analysis of lactate and glutamate distributions to delineate heterogeneity between mouse tumor models used to support drug discovery efficacy testing. We evaluate and report on the pre-analysis stabilization methods aimed at improving reproducibility and efficiency of quantitative assessment of endogenous molecules in tissues. Stability experiments demonstrate that optimum stabilization protocols consists of frozen tissue embedding, post tissue-sectioning desiccation and storage of tissue sections at -80°C sealed in vacuum tight containers...
April 18, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29667228/the-anterior-longitudinal-ligament-in-diffuse-idiopathic-skeletal-hyperostosis-ossified-or-displaced
#15
Jonneke S Kuperus, Esther Jm Smit, Behdad Pouran, Robbert W van Hamersvelt, Marijn van Stralen, Peter R Seevinck, Constantinus F Buckens, Ronald Law Bleys, Harrie H Weinans, F Cumhur Oner, Pim A de Jong, Jorrit-Jan Verlaan
Diffuse idiopathic skeletal hyperostosis (DISH) is often theorized to be an ossification of the anterior longitudinal ligament (ALL). Using computed tomography (CT) imaging and cryomacrotome sectioning we investigated the spatial relationship between the ALL and newly formed bone in DISH to test this hypothesis. In the current study, four human cadaveric spines diagnosed with DISH using CT imaging were frozen and sectioned using a cryomacrotome. Photographs were obtained of the specimen at 125µm intervals...
April 17, 2018: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/29665453/can-concurrent-high-risk-endometrial-carcinoma-occur-with-atypical-endometrial-hyperplasia
#16
Baki Erdem, Osman Aşıcıoğlu, Niyazi Alper Seyhan, Nuri Peker, Volkan Ülker, Özgür Akbayır
BACKGROUND: This study investigated the frequency of high-risk cancer types in hysterectomy material obtained from patients who were diagnosed with atypical endometrial hyperplasia (AEH) by endometrial sampling. MATERIALS AND METHODS: A total of 227 patients with AEH were retrospectively included in the study. Hysterectomy material was examined as both perioperative frozen section (FS) and paraffin-embedded permanent section (PS). Grade III tumors, grade II tumors larger than 2 cm, over 50% myometrial invasion, cervical involvement, and serous or clear cell histology were considered high-risk...
April 14, 2018: International Journal of Surgery
https://www.readbyqxmd.com/read/29662718/is-the-measurement-of-the-size-of-uterine-lesions-with-positron-emission-tomography-consistent-in-pre-and-postmenopausal-periods-in-endometrioid-type-endometrial-cancer
#17
Varol Gülseren, Mustafa Kocaer, Özgü Çelikkol Güngördük, İsa Aykut Özdemir, Muzaffer Sancı, Kemal Güngördük
Objective: We aimed to investigate the correlation of the size and volume of uterine tumors obtained using positron emission tomography/computed tomography (PET/CT) and pathology specimens in patients with endometrioid-type endometrial cancer (EEC) in the premenopausal period, and to compare the results with those of postmenopausal women. In the premenopausal period, the endometrium uses more glucose than in the postmenopausal period. Therefore, the measurement of uterine tumor size using PET/CT in the premenopausal period may normally be different...
March 2018: Turkish Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/29660577/value-and-best-way-for-detection-of-sentinel-lymph-node-in-early-stage-endometrial-cancer-selective-lymphadenectomy-algorithm
#18
Ahmed Samy El-Agwany, Mahmoud Hanafy Meleis
INTRODUCTION: The cornerstone of treatment for endometrial carcinoma is total abdominal hysterectomy and bilateral salpingo-oophoprectomy. Pelvic lymphadenectomy, with or without para-aortic lymphadenectomy, plays an important role in the surgical staging of endometrial carcinoma, and provides more accurate prognostic information. AIMS: We aimed to evaluate the value and techniques for SLN mapping in early stage endometrial cancer with low risk for lymph node metastasis and whether selective or systematic lymphadenectomy is needed according to the results of proposed algorithm...
March 23, 2018: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/29660167/muse-microscopy-with-uv-surface-excitation-a-novel-approach-to-real-time-inexpensive-slide-free-dermatopathology
#19
Amir Qorbani, Farzad Fereidouni, Richard Levenson, Sana Yasmine Lahoubi, Zachary T Harmany, Austin Todd, Maxwell A Fung
Traditional histology relies on processing and physically sectioning either frozen or formalin-fixed, paraffin-embedded (FFPE) tissue into thin slices (typically 4-6 microns) prior to staining and viewing on a standard wide-field microscope. MUSE represents a novel alternative microscopy method that works with ultraviolet (UV) excitation using oblique cis-illumination which can generate high-quality images from the cut surface of fresh or fixed tissue after brief staining, with no requirement for fixation, embedding and histological sectioning of tissue specimens...
April 16, 2018: Journal of Cutaneous Pathology
https://www.readbyqxmd.com/read/29659402/features-of-irradiated-skeletal-muscle-on-mohs-frozen-section-examination
#20
H William Higgins, Kachiu C Lee, Gladys Telang
No abstract text is available yet for this article.
April 13, 2018: Dermatologic Surgery: Official Publication for American Society for Dermatologic Surgery [et Al.]
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