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https://www.readbyqxmd.com/read/28101341/influence-of-high-altitude-hypoxic-environments-on-the-survival-of-cochlear-hair-cells-and-spiral-ganglion-neurons-in-rats
#1
Dongyan Fan, Hailong Ren, Dunzhu Danzeng, Haonan Li, Ping Wang
The aim of the present study was to observe the histological changes in the peripheral auditory system in rats at different time-points after relocating from low altitude to high altitude (3,600 m). The general physical condition of the rats was observed and cochlear tissue samples were obtained every month. The morphology and survival of the cochlear hair cells (HCs) were observed using cochlear surface preparation at 1, 30, 90, 120, 150 and 180 days after moving to the plateau area. Changes in spiral ganglion neurons (SGNs) were detected at different time-points using immunofluorescence technology on frozen sections...
December 2016: Biomedical Reports
https://www.readbyqxmd.com/read/28100570/a-rare-cause-of-constrictive-pericarditis
#2
Shane Patrick Flood, Omar Ayah, Satoshi Furukawa, Robert B Norris
A 67-year-old man presented with 3 months of exertional dyspnoea and 1 week of oedema. Examination revealed elevated neck veins, pulsus paradoxus, muffled heart sounds, decreased breath sounds and pedal oedema. Transthoracic echocardiogram (TTE) demonstrated cardiac tamponade, and chest X-ray showed pleural effusion. Pericardiocentesis, thoracocentesis, laboratory investigations and CT did not elucidate an underlying aetiology. Three weeks later, he presented with recurrent cardiac tamponade and pleural effusion...
January 18, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28099230/primary-orbital-chondromyxoid-fibroma-a-rare-case
#3
Martin G Mullen, Marie Somogyi, Sean P Maxwell, Vikram Prabhu, David K Yoo
A 56-year-old male with history of chronic sinusitis was found to have a 3 cm left orbital lesion on CT. Subsequent MRI demonstrated a multilobulated enhancing soft tissue lesion at the superotemporal region of the left orbit. Initial biopsy was reported as a low-grade sarcoma. On further evaluation, a consensus was made that the lesion was likely a benign mixed mesenchymal type tumor but should nonetheless be surgically removed. Left lateral orbitotomy was performed which revealed a tumor originating in the lateral orbital bone with segments eroding through the wall of the orbit...
January 17, 2017: Ophthalmic Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28097784/reliability-of-intraoperative-frozen-section-for-the-diagnosis-of-renal-tumors-suspicious-for-malignancy-in-children-and-adolescents
#4
Alonso Carrasco, Brian T Caldwell, Carrye R Cost, Brian S Greffe, Timothy P Garrington, Jennifer L Bruny, Jennifer O Black, Nicholas G Cost
BACKGROUND: The ability of intraoperative frozen section (IFS) to reliably diagnose renal tumors in children and adolescents is largely unknown. The objective of our study is to evaluate the ability of IFS to establish a histologic diagnosis for renal tumors in this population. METHODS: We reviewed our experience with patients who underwent IFS at the time of surgery for a renal tumor suspicious for malignancy from 2005 to 2015. The IFS was compared to the final pathology (FP)...
January 18, 2017: Pediatric Blood & Cancer
https://www.readbyqxmd.com/read/28095042/gauging-the-extent-of-thyroidectomy-for-indeterminate-thyroid-nodules-an-oncologic-perspective
#5
David F Schneider, Linda M Cherney Stafford, Nicole Brys, Caprice C Greenberg, Courtney J Balentine, Dawn M Elfenbein, Susan C Pitt
INTRODUCTION: Increasing emphasis is being placed on appropriateness of care and avoidance of over- and under-treatment. Indeterminate thyroid nodules (ITN), present a particular risk for this problem because cancer found via diagnostic lobectomy (DL) often requires a completion thyroidectomy (CT). However, initial total thyroidectomy (TT) for benign ITN results in lifelong thyroid hormone replacement. We sought to measure the accuracy and factors associated with the extent of initial thyroidectomy for ITN...
January 17, 2017: Endocrine Practice
https://www.readbyqxmd.com/read/28093272/a-method-for-obtaining-simian-immunodeficiency-virus-rna-sequences-from-laser-capture-microdissected-and-immune-captured-cd68-and-cd163-macrophages-from-frozen-tissue-sections-of-bone-marrow-and-brain
#6
Jaclyn Mallard, Emily Papazian, Caroline Soulas, David J Nolan, Marco Salemi, Kenneth C Williams
Laser capture microdissection (LCM) is used to extract cells or tissue regions for analysis of RNA, DNA or protein. Several methods of LCM are established for different applications, but a protocol for consistently obtaining lentiviral RNA from LCM captured immune cell populations is not described. Obtaining optimal viral RNA for analysis of viral genes from immune-captured cells using immunohistochemistry (IHC) and LCM is challenging. IHC protocols have long antibody incubation times that increase risk of RNA degradation...
January 13, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28093141/re-utility-and-significance-of-ureteric-frozen-section-analysis-during-radical-cystectomy
#7
Sam S Chang
No abstract text is available yet for this article.
February 2017: Journal of Urology
https://www.readbyqxmd.com/read/28089110/recurrence-after-management-of-meatal-balanitis-xerotica-obliterans
#8
Warren Snodgrass, Juan Soto Blanquel, Nicol Corbin Bush
INTRODUCTION: We review outcomes after management of meatal balanitis xerotica obliterans (BXO). The primary outcome was recurrent meatal BXO. METHODS: A database comprising mostly hypospadias patients was queried for meatal BXO. The disease was confirmed histologically in all cases. Management included topical steroids and/or immunosuppressants, and/or surgical excision of BXO with two-stage oral mucosa graft circumferential replacement urethroplasty. RESULTS: A total of 12 patients had meatal BXO (8 boys and 4 adults)...
November 23, 2016: Journal of Pediatric Urology
https://www.readbyqxmd.com/read/28088959/-effect-of-exendin-4-on-lipid-deposition-in-skeletal-muscle-of-diet-induced-obese-mice-and-its-underlying-mechanism
#9
H Y Cao, F Xu, Z L Chen, B S Lin, X B Zheng, S H Yuan, H Liang, J P Weng
Objective: To investigate the effect of exendin-4, a glucagon-like peptide-1 (GLP-1) receptor agonist, on reducing lipid deposition and improving insulin resistance in skeletal muscle and the underlying mechanisms in high-fat diet (HFD)-induced obese mice. Methods: Twelve male C57BL/6J mice were challenged with HFD for 12 weeks to induce obesity and then randomly divided into two groups: exendin-4 group (intraperitoneal injection of 24 nmol·kg(-1)·d(-1) exendin-4 for 4 weeks) and HFD group (intraperitoneal injection of normal saline for 4 weeks), with 6 mice in each group...
January 10, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/28087299/when-partial-nephrectomy-is-unsuccessful-understanding-the-reasons-for-conversion-from-robotic-partial-to-radical-nephrectomy-at-a-tertiary-referral-center
#10
Önder Kara, Matthew J Maurice, Pascal Mouracade, Ercan Malkoç, Julien Dagenais, Ryan J Nelson, Jaya Sai Chavali, Robert J Stein, Amr Fergany, Jihad H Kaouk
OBJECTIVE: To identify the preoperative factors associated with conversion from robotic partial nephrectomy (RPN) to radical nephrectomy (RN), and to report the incidence of this event. PATIENTS AND METHODS: Using our institutional review board-approved database, we abstracted data on 1023 RPN cases performed at our center between 2010 and 2015. Standard and converted cases were compared in terms of patients and tumor characteristics and perioperative, functional, and oncological outcomes...
January 10, 2017: Journal of Urology
https://www.readbyqxmd.com/read/28080173/evaluation-of-long-term-cryostorage-of-brain-tissue-sections-for-quantitative-histochemistry
#11
Larissa I Estrada, Amy A Robinson, Ana C Amaral, Eustathia L Giannaris, Nadine C Heyworth, Farzad Mortazavi, Laura B Ngwenya, Debra E Roberts, Howard J Cabral, Ronald J Killiany, Douglas L Rosene
Storage of tissue sections for long periods allows multiple samples, acquired over months or years, to be processed together, in the same reagents, for quantitative histochemical studies. Protocols for freezer storage of free-floating frozen sections using sucrose with different additives have been reported and assert that storage has no effect on histochemistry, but no quantitative support has been provided. The present study analyzed the efficacy of long-term storage of brain tissue sections at -80C in buffered 15% glycerol...
January 1, 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/28079788/the-prevalence-of-factor-viii-and-ix-inhibitors-among-saudi-patients-with-hemophilia-results-from-the-saudi-national-hemophilia-screening-program
#12
Tarek Owaidah, Abdulkareem Al Momen, Hazzaa Alzahrani, Abdulrahman Almusa, Fawaz Alkasim, Ahmed Tarawah, Randa Al Nouno, Fatima Al Batniji, Fahad Alothman, Ali Alomari, Saud Abu-Herbish, Mahmoud Abu-Riash, Khawar Siddiqui, Mansor Ahmed, S Y Mohamed, Mahasen Saleh
Hemophilia A and B are X-linked diseases that predominantly affect male patients. Patients can develop coagulation factor inhibitors, which exponentially increases the treatment cost. However, the prevalence of factor VIII and IX inhibitors in Saudi Arabia is unclear.This study aimed to determine the Saudi prevalence of factor VIII and IX inhibitors.This 4-year, 7-center, cross-sectional study evaluated the Saudi prevalences of hemophilia A and B. We collected the patients' clinical data, evaluated their disease, and tested for factor inhibitors...
January 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28075160/can-we-accomplish-better-oncological-results-with-robot-assisted-radical-prostatectomy
#13
Ali Riza Kural, Can Obek, Tünkut Doğanca
Surgical removal with radical prostatectomy has been a cornerstone for the treatment of prostate cancer and is associated with level one evidence for survival advantage compared to watchful waiting. Since the first structured robotic program was launched in 2000, robot-assisted radical prostatectomy (RARP) has had a rapid diffusion and surpassed its open (ORP) and laparoscopic (LRP) counterparts in the United States and is progressively expanding in other countries. Interestingly, this common acceptance of RARP was initially driven in the paucity of robust clinical evidence...
January 11, 2017: Journal of Endourology
https://www.readbyqxmd.com/read/28074002/metabolic-profiling-in-formalin-fixed-and-paraffin-embedded-prostate-cancer-tissues
#14
Stefano Cacciatore, Giorgia Zadra, Clyde Bango, Kathryn L Penney, Svitlana Tyekucheva, Oscar Yanes, Massimo Loda
: Metabolite profiling has significantly contributed to a deeper understanding of the biochemical metabolic networks and pathways in cancer cells. Metabolomics-based biomarker discovery would greatly benefit from the ability to interrogate retrospective annotated clinical specimens archived as formalin-fixed paraffin-embedded (FFPE) material. Mass spectrometry-based metabolomic analysis was performed in matched frozen and FFPE human prostate cancers as well as isogenic prostate cancer cell lines...
January 10, 2017: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/28073205/rare-presentation-of-a-testicular-angiofibroma-treated-with-testis-sparing-surgery
#15
Luca Leone, Paola Fulvi, Giulia Sbrollini, Alessandra Filosa, Enrico Caraceni, Angelo Marronaro, Andrea B Galosi
INTRODUCTION: Testicular benign tumors are very rare (< 5%). Testicular Angiofibroma (AF) is one of those, however the gold standard of treatment and follow-up is still unclear. CASE REPORT: A 47 years-old man with only one functioning testis was referred to our clinic for a palpable right testicular mass and atrophic contralateral testis. Patient underwent testis-sparing surgery with inguinal approach and intraoperative frozen sections examination with diagnosis of AF...
December 30, 2016: Archivio Italiano di Urologia, Andrologia
https://www.readbyqxmd.com/read/28073203/testicular-sparing-surgery-in-small-testis-masses-a-multinstitutional-experience
#16
Andrea B Galosi, Paola Fulvi, Andrea Fabiani, Lucilla Servi, Alessandra Filosa, Luca Leone, Angelo Marronaro, Enrico Caraceni, Rodolfo Montironi
INTRODUCTION: The incidence of benign testicular tumors is increasing in particular in small lesion incidentally found at scrotal ultrasonography. Primary aim of this study was to perform radical surgery in malignant tumor. Secondary aim was to verify the efficacy of the diagnostic-therapeutic pathway recently adopted in management of small masses with testis sparing surgery in benign lesions. MATERIALS AND METHODS: In this multicenter study, we reviewed all patients with single testis lesion less than 15 mm at ultrasound as main diameter...
December 30, 2016: Archivio Italiano di Urologia, Andrologia
https://www.readbyqxmd.com/read/28071617/role-of-frozen-section-in-the-intra-operative-margin-assessment-during-breast-conserving-surgery
#17
K R Anila, K Chandramohan, A Mathews, T Somanathan, K Jayasree
BACKGROUND: Breast conserving surgery (BCS) is increasingly done for early breast cancers in many countries since it has been demonstrated by randomized trials that survival rates after BCS followed by adjuvant therapy are equivalent to those obtained after mastectomy. Frozen section analyses (FSA) is a technique used for intra-operative assessment of margin status in BCS. The aim of this study was to assess the concordance of margin status assessment by FSA and permanent sections and to assess correlation with local recurrence...
April 2016: Indian Journal of Cancer
https://www.readbyqxmd.com/read/28069517/fast-estimation-of-colles-fracture-load-of-the-distal-section-of-the-radius-by-homogenized-finite-element-analysis-based-on-hr-pqct
#18
Hadi S Hosseini, Andreas Dünki, Jonas Fabech, Martin Stauber, Nicolas Vilayphiou, Dieter Pahr, Michael Pretterklieber, Jasmin Wandel, Bert van Rietbergen, Philippe K Zysset
Fractures of the distal section of the radius (Colles' fractures) occur earlier in life than other osteoporotic fractures. Therefore, they can be interpreted as a warning signal for later, more deleterious fractures of vertebral bodies or the femoral neck. In the past decade, the advent of HR-pQCT allowed a detailed architectural analysis of the distal radius and an automated but time-consuming estimation of its strength with linear micro-finite element (μFE) analysis. Recently, a second generation of HR-pQCT scanner (XtremeCT II, SCANCO Medical, Switzerland) with a resolution beyond 61 μm became available for even more refined biomechanical investigations in vivo...
January 7, 2017: Bone
https://www.readbyqxmd.com/read/28062679/posterior-uniportal-video-assisted-thoracoscopic-surgery-for-resection-of-the-apical-segment-of-the-right-lower-lobe-followed-by-completion-lobectomy
#19
Davor Stamenovic, Antje Messerschmidt
Uniportal thoracoscopic resections offer less pain and better cosmetic results. They are usually performed through an antero-lateral incision. Posterior uniportal approach has not been described yet. A 65-year-old female was admitted to our clinic for the treatment of an adenocarcinoma, located in the apical segment of the right lower lobe. Owing to the ideal location and size, anatomical segment resection and radical lymphadenectomy was planned. Fissureless video-assisted thoracoscopic resection of the apical segment of the right lower lobe, using a uniportal posterior approach was performed, followed by mediastinal lymphadenectomy...
January 6, 2017: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/28060874/salmon-dna-accelerates-bone-regeneration-by-inducing-osteoblast-migration
#20
Ayako Sato, Hiroshi Kajiya, Nana Mori, Hironobu Sato, Tadao Fukushima, Hirofumi Kido, Jun Ohno
The initial step of bone regeneration requires the migration of osteogenic cells to defective sites. Our previous studies suggest that a salmon DNA-based scaffold can promote the bone regeneration of calvarial defects in rats. We speculate that the salmon DNA may possess osteoinductive properties, including the homing of migrating osteogenic cells. In the present study, we investigated the influence of the salmon DNA on osteoblastic differentiation and induction of osteoblast migration using MG63 cells (human preosteoblasts) in vitro...
2017: PloS One
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