Justine Lallement, Ilyès Raho, Grégory Merlen, Dominique Rainteau, Mikael Croyal, Melody Schiffano, Nadim Kassis, Isabelle Doignon, Maud Soty, Floriane Lachkar, Michel Krempf, Matthias Van Hul, Patrice D Cani, Fabienne Foufelle, Chloé Amouyal, Hervé Le Stunff, Christophe Magnan, Thierry Tordjmann, Céline Cruciani-Guglielmacci
Ceramides (Cer) have been shown as lipotoxic inducers, which disturb numerous cell-signaling pathways, leading to metabolic disorders such as type 2 diabetes. In this study, we aimed to determine the role of de novo hepatic ceramide synthesis in energy and liver homeostasis in mice. We generated mice lacking serine palmitoyltransferase 2 (Sptlc2), the rate limiting enzyme of ceramide de novo synthesis, in liver under albumin promoter. Liver function, glucose homeostasis, bile acid (BA) metabolism and hepatic sphingolipids content were assessed using metabolic tests and LC-MS...
May 22, 2023: Biochimica et Biophysica Acta. Molecular and Cell Biology of Lipids