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periodontal ligament fibers

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https://www.readbyqxmd.com/read/28326701/-morphological-features-and-analysis-of-the-nerve-fibers-in-the-periodontal-ligament-of-dogs
#1
Zeng Xiaohua, Cai Qiaoling, Gong Ping
OBJECTIVE: To identify the properties of nerve fibers of dogs by immunohistochemical staining method. METHODS: Intact bone blocks above the inferior alveolar nerve canal were cut from the medial of the second premolar to the distal of the third premolar of healthy adult Beagle dogs of 18 months, embedded to make hard tissue sections, stained with S100 and neurofilament protein (NFP) antibodies, and finally observed the nerve distribution under the microscope. RESULTS: The distribution of S100 positive tissue in the periodontal ligament of dogs showed the following patterns: bundles of densely gathered rings with different diameters, filaments accompanied by lumens, free endings and deep-dyeing oval lamellasome...
October 1, 2016: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/28325578/first-evidence-for-regeneration-of-the-periodontium-to-mineral-trioxide-aggregate-in-human-teeth
#2
Andreas Bartols, Eleni Roussa, Winfried Walther, Christof E Dörfer
INTRODUCTION: The aim of this study was to elucidate whether the use of mineral trioxide aggregate (MTA) in endodontic therapy in human teeth leads to the same regeneration of the apical tissues as observed in animals. METHODS: Four human teeth were identified in a policlinic that had been treated endodontically with MTA and had to be extracted for other reasons than just endodontic failure. All teeth were processed for histologic and one for immunohistochemical analyses to analyze the histologic response of the periapical structure to the former treatment with MTA...
March 18, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28246503/ultrasonographic-evaluation-of-periodontal-changes-during-orthodontic-tooth-movement-work-in-progress
#3
Adela Zimbran, Diana Dudea, Cristina Gasparik, Sorin Dudea
BACKGROUND AND AIM: Orthodontic tooth movement (OTM) is a process whereby the application of a force induces bone resorption on the pressure side and bone apposition on the tension side of the lamina dura. However, only limited data are available on the in vivo behavior of the periodontal tissues. The aim of this study was to assess the changes of periodontal tissues, induced by the orthodontic canine retraction, using 40 MHz ultrasonography. METHODS: Ultrasonographic evaluation of periodontal tissues was conducted in 5 patients with indication for orthodontic treatment...
2017: Clujul Medical (1957)
https://www.readbyqxmd.com/read/28245286/sparc-and-the-n-propeptide-of-collagen-i-influence-fibroblast-proliferation-and-collagen-assembly-in-the-periodontal-ligament
#4
Emilie Moore Rosset, Jessica Trombetta-eSilva, Glenn Hepfer, Hai Yao, Amy Dodd Bradshaw
The periodontal ligament (PDL) is a fibrous connective tissue that anchors tooth cementum into alveolar bone. Secreted protein acidic and rich in cysteine (SPARC) is a collagen-binding matricellular protein known to influence collagen fiber assembly in the PDL. In contrast, functional properties of the N-propeptide of collagen I, encoded in exon 2 of the COL1A1 gene, are poorly understood. In this study, the PDL of collagen I exon 2-deleted (wt/ko), SPARC-null (ko/wt), and double transgenic (ko/ko) mice were evaluated in terms of cellularity, collagen area, fiber morphology, and extraction force and compared to WT (wt/wt) mice...
2017: PloS One
https://www.readbyqxmd.com/read/28208578/role-of-cortico-cancellous-heterologous-bone-in-human-periodontal-ligament-stem-cell-xeno-free-culture-studied-by-synchrotron-radiation-phase-contrast-microtomography
#5
Serena Mazzoni, Sara Mohammadi, Giuliana Tromba, Francesca Diomede, Adriano Piattelli, Oriana Trubiani, Alessandra Giuliani
This study was designed to quantitatively demonstrate via three-dimensional (3D) images, through the Synchrotron Radiation Phase-Contrast Microtomography (SR-PhC-MicroCT), the osteoinductive properties of a cortico-cancellous scaffold (Osteobiol Dual Block-DB) cultured with human Periodontal Ligament Stem Cells (hPDLSCs) in xeno-free media. In vitro cultures of hPDLSCs, obtained from alveolar crest and horizontal fibers of the periodontal ligament, were seeded onto DB scaffolds and cultured in xeno-free media for three weeks...
February 10, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28191762/periodontal-tissue-regeneration-using-syngeneic-adipose-derived-stromal-cells-in-a-mouse-model
#6
Mathieu Lemaitre, Paul Monsarrat, Vincent Blasco-Baque, Pascale Loubières, Rémy Burcelin, Louis Casteilla, Valérie Planat-Bénard, Philippe Kémoun
Current treatment of periodontitis is still associated with a high degree of variability in clinical outcomes. Recent advances in regenerative medicine by mesenchymal cells, including adipose stromal cells (ASC) have paved the way to improved periodontal regeneration (PD) but little is known about the biological processes involved. Here, we aimed to use syngeneic ASCs for periodontal regeneration in a new, relevant, bacteria-induced periodontitis model in mice. Periodontal defects were induced in female C57BL6/J mice by oral gavage with periodontal pathogens...
February 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28136695/heterogeneous-human-periodontal-ligament-committed-progenitor-and-stem-cell-populations-exhibit-a-unique-cementogenic-property-under-in-vitro-and-in-vivo-conditions
#7
Rei Shinagawa-Ohama, Mai Mochizuki, Yuichi Tamaki, Naoto Suda, Taka Nakahara
An undesirable complication that arises during dental treatments is external apical-root resorption, which causes root-cementum and root-dentin loss. To induce de novo cementogenesis, stem cell therapy is required. Cementum-forming cells (cementoblasts) are known to be differentiated from periodontal-lineage mesenchymal stem cells (MSCs), which are derived from the dental follicle (DF) in developing tissues and the periodontal ligament (PDL) in adult tissues, but the periodontal-lineage MSC type that is optimal for inducing de novo cementogenesis remains unidentified, as does the method to isolate these cells from harvested tissues...
January 31, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28081294/xenogeneic-bio-root-prompts-the-constructive-process-characterized-by-macrophage-phenotype-polarization-in-rodents-and-nonhuman-primates
#8
Hui Li, Jingjing Sun, Jie Li, Hefeng Yang, Xiangyou Luo, Jinlong Chen, Li Xie, Fangjun Huo, Tian Zhu, Weihua Guo, Weidong Tian
Tissue or organ regeneration using xenogeneic matrices is a promising approach to address the shortage of donor matrices for allotransplantation. Success of such approach has been demonstrated to correlate with macrophage-mediated fibrotic homeostasis and tissue remodeling. The previous studies have demonstrated that treated dentin matrix (TDM) could be a suitable bioactive substrate for allogeneic tooth root regeneration. This study constructed xenogeneic bioengineered tooth root (bio-root) via a combination of porcine TDM (pTDM) with allogeneic dental follicle cells (DFCs)...
January 12, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28053317/jawbone-microenvironment-promotes-periodontium-regeneration-by-regulating-the-function-of-periodontal-ligament-stem-cells
#9
Bin Zhu, Wenjia Liu, Yihan Liu, Xicong Zhao, Hao Zhang, Zhuojing Luo, Yan Jin
During tooth development, the jawbone interacts with dental germ and provides the development microenvironment. Jawbone-derived mesenchymal stem cells (JBMSCs) maintain this microenvironment for root and periodontium development. However, the effect of the jawbone microenvironment on periodontium tissue regeneration is largely elusive. Our previous study showed that cell aggregates (CAs) of bone marrow mesenchymal stem cells promoted periodontium regeneration on the treated dentin scaffold. Here, we found that JBMSCs enhanced not only the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) but also their adhesion to titanium (Ti) material surface...
January 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/27936010/new-insights-on-the-composition-and-the-structure-of-the-acellular-extrinsic-fiber-cementum-by-raman-analysis
#10
Thomas Colard, Guillaume Falgayrac, Benoit Bertrand, Stephan Naji, Olivier Devos, Clara Balsack, Yann Delannoy, Guillaume Penel
Acellular extrinsic fiber cementum is a mineralized tissue that covers the cervical half of the tooth root surface. It contains mainly extrinsic or Sharpey's fibers that run perpendicular to the root surface to anchor the tooth via the periodontal ligament. Acellular cementum is continuously and slowly produced throughout life and exhibits an alternating bright and dark pattern under light microscopy. However, although a better understanding of the structural background of acellular cementum is relevant to many fields, such as cementochronology, periodontology and tissue engineering, acellular cementum remains rarely studied and poorly understood...
2016: PloS One
https://www.readbyqxmd.com/read/27881487/osteogenesis-requires-fak-dependent-collagen-synthesis-by-fibroblasts-and-osteoblasts
#11
Dhaarmini Rajshankar, Yongqiang Wang, Christopher A McCulloch
Focal adhesion kinase (FAK) is critical in adhesion-dependent signaling, but its role in osteogenesis in vivo is ill defined. We deleted Fak in fibroblasts and osteoblasts in Floxed-Fak mice bred with those expressing Cre-recombinase driven by 3.6 kb α1(I)-collagen promoter. Compared with wild-type (WT), conditional FAK-knockout (CFKO) mice were shorter (2-fold; P < 0.0001) and had crooked, shorter tails (50%; P < 0.0001). Microcomputed tomography analysis showed reduced bone volume (4-fold in tails; P < 0...
November 23, 2016: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/27761392/periosteum-as-a-lateral-pedicle-graft-for-the-treatment-of-single-tooth-root-recession-a-novel-approach
#12
Avadhesh Kumar, Shalini Kaushal, Neelu Verma, Deepti Chandra
The treatment of gingival recession defects is indicated for esthetic and functional reasons to reduce root sensitivity, to remove muscle pull, to create or augment keratinized tissue, and to prevent disease progression. The presence of sufficient amount of periosteum adjacent to gingival recession defects makes it a suitable graft. The adult human periosteum is a highly vascular connective tissue with immense regenerative potential. It contains fibroblasts, osteogenic progenitor cells, and stem cells as a result of which it has the ability to differentiate into fibroblast, osteoblast, chondrocytes, adipocytes, and skeletal myocytes...
September 2016: Journal of Oral Biology and Craniofacial Research
https://www.readbyqxmd.com/read/27748036/effects-of-diode-laser-irradiation-and-fibroblast-growth-factor-on-periodontal-healing-of-replanted-teeth-after-extended-extra-oral-dry-time
#13
Erica Dos Santos Carvalho, Rogério Hadid Rosa, Flávia de Moura Pereira, Ana Lia Anbinder, Isabel Mello, Sandra Marcia Habitante, Denise Pontes Raldi
AIM: The search for effective protocols to reduce the incidence of root resorption and allow periodontal ligament repair is still challenging, given the unpredictable outcome of late tooth replantation. The aim of this study was to assess the effects of both high-power diode laser irradiation (DL) and basic fibroblast growth factor (FGF) on the periodontal healing of replanted teeth after extended extra-oral dry time. METHODS: Maxillary incisors of 50 male rats were extracted and assigned to three experimental and two control groups (n = 10)...
April 2017: Dental Traumatology: Official Publication of International Association for Dental Traumatology
https://www.readbyqxmd.com/read/27672630/a-nano-indentation-identification-technique-for-viscoelastic-constitutive-characteristics-of%C3%A2-periodontal-ligaments
#14
H Ashrafi, M Shariyat
INTRODUCTION: Nano-indentation has recently been employed as a powerful tool for determining the mechanical properties of biological tissues on nano and micro scales. A majority of soft biological tissues such as ligaments and tendons exhibit viscoelastic or time-dependent behaviors. The constitutive characterization of soft tissues is among very important subjects in clinical medicine and especially, biomechanics fields. Periodontal ligament plays an important role in initiating tooth movement when loads are applied to teeth with orthodontic appliances...
June 2016: Journal of Biomedical Physics & Engineering
https://www.readbyqxmd.com/read/27638921/periodontal-tissue-regeneration-using-syngeneic-adipose-derived-stromal-cells-in-a-mouse-model
#15
Mathieu Lemaitre, Paul Monsarrat, Vincent Blasco-Baque, Pascale Loubières, Rémy Burcelin, Louis Casteilla, Valérie Planat-Bénard, Philippe Kémoun
: Current treatment of periodontitis is still associated with a high degree of variability in clinical outcomes. Recent advances in regenerative medicine by mesenchymal cells, including adipose stromal cells (ASC) have paved the way to improved periodontal regeneration (PD) but little is known about the biological processes involved. Here, we aimed to use syngeneic ASCs for periodontal regeneration in a new, relevant, bacteria-induced periodontitis model in mice. Periodontal defects were induced in female C57BL6/J mice by oral gavage with periodontal pathogens...
September 16, 2016: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/27600358/the-differentiation-potential-of-gingival-mesenchymal-stem-cells-induced-by-apical-tooth-germ-cell%C3%A2-conditioned-medium
#16
Yan Chen, Hongwei Liu
Gingival-derived mesenchymal stem cells (GMSCs) have recently been harvested; however, the use of GMSCs in periodontal tissue engineering requires further study. The present study established an indirect co‑culture system between rat apical tooth germ‑conditioned medium (APTG‑CM) and GMSCs, in order to determine the effects on periodontal tissue differentiation in vitro and in vivo. Using the limiting dilution technique, single‑colony derived human GMSCs and periodontal ligament stem cells (PDLSCs) were isolated and expanded to obtain homogeneous populations...
October 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27583044/collagen-hydrogel-scaffold-and-fibroblast-growth-factor-2-accelerate-periodontal-healing-of-class-ii-furcation-defects-in-dog
#17
Takehito Momose, Hirofumi Miyaji, Akihito Kato, Kosuke Ogawa, Takashi Yoshida, Erika Nishida, Syusuke Murakami, Yuta Kosen, Tsutomu Sugaya, Masamitsu Kawanami
OBJECTIVE: Collagen hydrogel scaffold exhibits bio-safe properties and facilitates periodontal wound healing. However, regenerated tissue volume is insufficient. Fibroblast growth factor-2 (FGF2) up-regulates cell behaviors and subsequent wound healing. We evaluated whether periodontal wound healing is promoted by application of collagen hydrogel scaffold in combination with FGF2 in furcation defects in beagle dogs. METHODS: Collagen hydrogel was fabricated from bovine type I collagen with an ascorbate-copper ion cross-linking system...
2016: Open Dentistry Journal
https://www.readbyqxmd.com/read/27407006/filamin-a-regulates-the-organization-and-remodeling-of-the-pericellular-collagen-matrix
#18
Masaru Mezawa, Vanessa I Pinto, Mwayi P Kazembe, Wilson S Lee, Christopher A McCulloch
Extracellular matrix remodeling by cell adhesion-related processes is critical for proliferation and tissue homeostasis, but how adhesions and the cytoskeleton interact to organize the pericellular matrix (PCM) is not understood. We examined the role of the actin-binding protein, filamin A (FLNa), in pericellular collagen remodeling. Compared with wild-type (WT), mice with fibroblast-specific deletion of FLNa exhibited higher density but reduced organization of collagen fibers after increased loading of the periodontal ligament for 2 wk...
October 2016: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/27280804/surface-chemistry-of-nanoscale-mineralized-collagen-regulates-periodontal-ligament-stem-cell-fate
#19
Yu Fu, Shuai Liu, Sheng-Jie Cui, Xiao-Xing Kou, Xue-Dong Wang, Xiao-Mo Liu, Yue Sun, Gao-Nan Wang, Yan Liu, Yan-Heng Zhou
The interplay between stem cells and their extracellular microenvironment is of critical importance to the stem cell-based therapeutics in regenerative medicine. Mineralized collagen is the main component of bone extracellular matrix, but the effect of interfacial properties of mineralized collagen on subsequent cellular behaviors is unclear. This study examined the role of surface chemistry of nanoscale mineralized collagen on human periodontal ligament stem cell (hPDLSC) fate decisions. The intrafibrillarly mineralized collagen (IMC), fabricated by a biomimetic bottom-up approach, showed a bonelike hierarchy with nanohydroxyapatites (HAs) periodically embedded within fibrils...
June 29, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27260210/removal-process-of-prion-and-parvovirus-from-human-platelet-lysates-used-as-clinical-grade-supplement-for-ex-vivo-cell-expansion
#20
Yu-Chun Kao, Andy Bailey, Bernhard Samminger, Junji Tanimoto, Thierry Burnouf
BACKGROUND AIMS: Pooled human platelet lysate (HPL) is becoming the new gold standard as supplement for ex vivo cell culture for clinical protocols. However, the risk of pathogen contamination of HPL increases with the platelet pool size. We hypothesized that hollow fiber anion exchange membrane chromatography using QyuSpeed D (QSD) could remove resistant and untested bloodborne pathogens, such as parvoviruses and prions, from HPL-supplemented growth media without substantially affecting their capacity to support ex vivo cell expansion...
July 2016: Cytotherapy
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