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https://www.readbyqxmd.com/read/28932956/time-lapse-imaging-provides-further-evidence-that-planar-arrangement-of-blastomeres-is-highly-abnormal
#1
Thomas Ebner, Alexandra Höggerl, Peter Oppelt, Elisabeth Radler, Simon-Hermann Enzelsberger, Richard B Mayer, Erwin Petek, Omar Shebl
PURPOSE: Recently, guidelines on the annotation of dynamic human embryo monitoring recommended screening for the presence of planar blastomere arrangement at the 4-cell stage. This observational study was set up in order to analyze whether developmental kinetics of planar human embryos are different from tetrahedral ones. METHODS: Therefore, embryos of 115 consecutive ICSI patients (showing 32 planar and 554 tetrahedral embryos) were cultured in a new time-lapse system (Miri TL) and their embryos were annotated for morphokinetic development and screened for irregular cleavages and morphological dysmorphisms...
September 20, 2017: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/28886319/morphokinetic-analysis-of-human-embryo-development-and-its-relationship-to-the-female-age-a-retrospective-time-lapse-imaging-study
#2
N Akhter, M Shahab
Time-lapse imaging technique has provided embryologists with a unique chance of studying the embryo morphokineticsto select the most viable embryos for implantation in the field of IVF (In vitro fertilization).The conventional method of morphological evaluations has proved that maternal age affects the human embryo quality. This retrospective study carried out at Islamabad Clinic Serving Infertile Couples, Islamabad, Pakistan mainly focuses on the effect of female age on human embryo morphokinetics. A total number of 200 patients undergoing ICSI treatment cycles at the clinic were selected for the study and divided into five age groups (< 26, 26-30, 31-35, 36-40, and > 40 years)...
August 30, 2017: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/28865549/effect-of-oocyte-vitrification-on-embryo-quality-time-lapse-analysis-and-morphokinetic-evaluation
#3
Ana Cobo, Aila Coello, Jose Remohí, Jose Serrano, Jose Maria de Los Santos, Marcos Meseguer
OBJECTIVE: To analyze whether oocyte vitrification may affect subsequent embryo development from a morphokinetic standpoint by means of time-lapse imaging. DESIGN: Observational cohort study. SETTING: University-affiliated private IVF center. PATIENT(S): Ovum donation cycles conducted with the use of vitrified (n = 631 cycles; n = 3,794 embryos) or fresh oocytes (n = 1,359 cycles; n = 9,935 embryos) over 2 years. INTERVENTIONS(S): None...
September 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28795048/evaluation-of-human-embryo-development-in-in-vitro-fertilization-and-intracytoplasmic-sperm-injection-fertilized-oocytes-a-time-lapse-study
#4
Hyung Jun Kim, Hye Jin Yoon, Jung Mi Jang, Won Don Lee, San Hyun Yoon, Jin Ho Lim
OBJECTIVE: We investigated whether the insemination method (in vitro fertilization [IVF] or intracytoplasmic sperm injection [ICSI]) affected morphokinetic events and abnormal cleavage events in embryonic development. METHODS: A total of 1,830 normal fertilized embryos were obtained from 272 IVF and ICSI cycles that underwent ovum retrieval culture using a time-lapse system (Embryoscope) from June 2013 to March 2015. All embryos were investigated by a detailed time-lapse analysis that measured the developmental events in the hours after IVF or ICSI insemination...
June 2017: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/28751285/comparison-of-human-embryomorphokinetic-parameters-in-sequential-or-global-culture-media
#5
Nadia Kazdar, Florence Brugnon, Cyril Bouche, Guilhem Jouve, Ségolène Veau, Hortense Drapier, Chloé Rousseau, Céline Pimentel, Patricia Viard, Marc-Antoine Belaud-Rotureau, Célia Ravel
A prospective study on randomized patients was conducted to determine how morphokinetic parameters are altered in embryos grown in sequential versus global culture media. Eleven morphokinetic parameters of 160 single embryos transferred were analyzed by time lapse imaging involving two University-affiliated in vitro fertilization (IVF) centers. We found that the fading of the two pronuclei occurred earlier in global (22.56±2.15 hpi) versus sequential media (23.63±2.71 hpi; p=0.0297). Likewise, the first cleavage started earlier at 24...
August 1, 2017: Annales de Biologie Clinique
https://www.readbyqxmd.com/read/28740447/breast-dynamic-contrast-enhanced-mri-fibrocystic-changes-presenting-as-a-non-mass-enhancement-mimicking-malignancy
#6
Zorica C Milosevic, Mirjan M Nadrljanski, Zorka M Milovanovic, Nina Z Gusic, Slavko S Vucicevic, Olga S Radulovic
BACKGROUND: We aimed to analyse the morphokinetic features of breast fibrocystic changes (nonproliferative lesions, proliferative lesions without atypia and proliferative lesions with atypia) presenting as a non-mass enhancement (NME)in dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) examination. PATIENTS AND METHODS: Forty-six patients with histologically proven fibrocystic changes (FCCs) were retrospectively reviewed, according to Breast Imaging Reporting and Data System (BI-RADS) lexicon...
June 2017: Radiology and Oncology
https://www.readbyqxmd.com/read/28736152/time-lapse-culture-with-morphokinetic-embryo-selection-improves-pregnancy-and-live-birth-chances-and-reduces-early-pregnancy-loss-a-meta-analysis
#7
REVIEW
Csaba Pribenszky, Anna-Maria Nilselid, Markus Montag
Embryo evaluation and selection is fundamental in clinical IVF. Time-lapse follow-up of embryo development comprises undisturbed culture and the application of the visual information to support embryo evaluation. A meta-analysis of randomized controlled trials was carried out to study whether time-lapse monitoring with the prospective use of a morphokinetic algorithm for selection of embryos improves overall clinical outcome (pregnancy, early pregnancy loss, stillbirth and live birth rate) compared with embryo selection based on single time-point morphology in IVF cycles...
July 10, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28712646/live-births-after-embryo-selection-using-morphokinetics-versus-conventional-morphology-a-retrospective-analysis
#8
Simon Fishel, Alison Campbell, Sue Montgomery, Rachel Smith, Lynne Nice, Samantha Duffy, Lucy Jenner, Kathyrn Berrisford, Louise Kellam, Rob Smith, Ivy D'Cruz, Ashley Beccles
The increasing corpus of clinical studies using time-lapse imaging for embryo selection demonstrates considerable variation in study protocols and only limited-sized study cohorts. Outcome measures are based on implantation or clinical pregnancy; some predict blastulation from early cleavage-stage data, and few have evaluated live birth. Erroneously, most studies treat the embryos as independent variables and do not include patient or treatment variables in the statistical analyses. In this study, cohort size was 14,793 patients and 23,762 cycles...
June 23, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28676910/morphokinetic-parameters-from-a-time-lapse-monitoring-system-cannot-accurately-predict-the-ploidy-of-embryos
#9
Jingye Zhang, Wenrong Tao, Hui Liu, Guanling Yu, Mei Li, Shuiying Ma, Keliang Wu
PURPOSE: This study aimed to test whether there is an association between embryo morphokinetic parameters and ploidy status. METHODS: Patients with high risk of aneuploidy were analyzed by time-lapse microscopy combined with preimplantation genetic screening (PGS). Accordingly, 256 blastocysts from 75 patients were subjected to trophectoderm biopsy and microarray comparative genomic hybridization (array-CGH). Blastocyst development process was analyzed using time-lapse images...
July 4, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28646146/optical-coherence-microscopy-as-a-novel-non-invasive-method-for-the-4d-live-imaging-of-early-mammalian-embryos
#10
Karol Karnowski, Anna Ajduk, Bartosz Wieloch, Szymon Tamborski, Krzysztof Krawiec, Maciej Wojtkowski, Maciej Szkulmowski
Imaging of living cells based on traditional fluorescence and confocal laser scanning microscopy has delivered an enormous amount of information critical for understanding biological processes in single cells. However, the requirement for a high numerical aperture and fluorescent markers still limits researchers' ability to visualize the cellular architecture without causing short- and long-term photodamage. Optical coherence microscopy (OCM) is a promising alternative that circumvents the technical limitations of fluorescence imaging techniques and provides unique access to fundamental aspects of early embryonic development, without the requirement for sample pre-processing or labeling...
June 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28643503/the-use-of-morphokinetic-as-a-predictor-of-implantation
#11
Alberto Tejera, Belén Aparicio-Ruiz, Marcos Meseguer
In recent years the increased efforts intended for improving future outcomes in the laboratory have focused mostly on the search of additional markers of embryo quality to add up present embryo selection criteria. Time-lapse system involves an alternative tool in assisted reproduction techniques, being able to improve the embryo selection from a dynamic and interactive approach while standard embryo assessment implies a subjective and static morphology evaluation and consequently reducing the information gained for embryo selection, time-lapse technology adds several morphokinetic parameters, providing additional input for embryo evaluation...
June 22, 2017: Minerva Ginecologica
https://www.readbyqxmd.com/read/28573526/selecting-embryos-with-the-highest-implantation-potential-using-data-mining-and-decision-tree-based-on-classical-embryo-morphology-and-morphokinetics
#12
Beatriz Carrasco, Gemma Arroyo, Yolanda Gil, Mª José Gómez, Ignacio Rodríguez, Pedro N Barri, Anna Veiga, Montserrat Boada
PURPOSE: The objective of this work was to determine which embryonic morphokinetic parameters up to D3 of in vitro development have predictive value for implantation for the selection of embryos for transfer in clinical practice based upon information generated from embryo transfers with known implantation data (KID). METHODS: A total of 800 KID embryos (100% implantation rate (IR) per transfer and 0% IR per transfer) cultured in an incubator with Time-Lapse system were retrospectively analysed...
August 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28470453/the-effect-of-follicle-size-and-homogeneity-of-follicular-development-on-the-morphokinetics-of-human-embryos
#13
Semra Kahraman, Caroline Pirkevi Cetinkaya, Murat Cetinkaya, Hakan Yelke, Yesim Kumtepe Colakoglu, Melih Aygun, Markus Montag
PURPOSE: Our aim was to investigate follicular size (large, ≥17 mm and small, <17 mm) at the time of OPU and homogeneity of follicular development (homogenous development: follicles being present in a homogenous spread of all sizes; heterogeneous: a predominance of small and large follicles) by analysing the morphokinetics of embryo development. METHODS: In this prospective cohort study, 2526 COCs belonging to 187 patients were cultured to day 5. Embryos were evaluated morphokinetically...
July 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28466179/is-there-any-correlation-between-oocyte-polarization-microscopy-findings-with-embryo-time-lapse-monitoring-in-icsi-program
#14
Azita Faramarzi, Mohammad Ali Khalili, Azam Agha-Rahimi, Marjan Omidi
PURPOSE: The aim was to investigate the relationship between the presence of the meiotic spindle (MS) and zona pellucida (ZP) birefringence of MII oocytes with morphokinetics variables of derived embryos in ICSI setting. METHODS: Using a polarization imaging system, the ZP birefringence and presence of MS were evaluated pre ICSI. Also, morphokinetics variables including time of second PB extrusion (tPB2), time of pronuclei appearance (tPNa), time of pronuclei fading (tPNf), time of two to eight discrete cells (t2-t8) ECC1 (t2-tPB2), cc2a (t3-t2), S2 (t4-t3) and S3 (t8-t5) as well as irregular cleavage events of 368 embryos were analyzed with time lapse monitoring (TLM)...
May 2, 2017: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/28455755/is-early-embryo-development-as-observed-by-time-lapse-microscopy-dependent-on-whether-fresh-or-frozen-sperm-was-used-for-icsi-a-cohort-study
#15
Jessica Eastick, Christos Venetis, Simon Cooke, Ashleigh Storr, Daisy Susetio, Michael Chapman
PURPOSE: The aim of this study was to compare timings of key events of embryo development from those originating from either fresh or cryopreserved ejaculate sperm using time-lapse technology. METHODS: In this retrospective observational cohort study, time-lapse technology was used to monitor 1927 embryos from 234 women undergoing intracytoplasmic sperm injection (ICSI) and utilizing either fresh (n = 172 cycles) or cryopreserved ejaculate sperm (n = 62 cycles) for insemination were included in the study...
June 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28455752/erratum-to-the-association-between-coenzyme-q10-concentrations-in-follicular-fluid-with-embryo-morphokinetics-and-pregnancy-rate-in-assisted-reproductive-techniques
#16
Süleyman Akarsu, Funda Gode, Ahmet Zeki Isik, Zeliha Günnur Dikmen, Mustafa Agah Tekindal
No abstract text is available yet for this article.
May 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28412866/comparison-of-the-morphokinetic-parameters-of-embryos-according-to-ovarian-reserve-in-ivf-cycles
#17
Suleyman Akarsu, Funda Gode, Ahmet Zeki Isik, Hayriye Celenk, Ferda Burcu Tamer, Selcuk Erkilinc
The aim of this study is to evaluate the impact of ovarian reserve and age of women on early morphokinetic parameters of embryos with a time-lapse monitoring system. In total, 197 infertile couples with poor ovarian reserve (Group 1, n = 41), normal ovarian reserve (Group 2, n = 59), or polycystic ovaries (Group 3, n = 97) were included. The time from insemination to the following events were analyzed: pronuclear fading (Pnf) and cleavage to 2, 3, 4 and 5 cells. The optimal ranges for morphokinetic parameters of t5, s2 and cc2 in each group were also evaluated...
April 17, 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/28408705/time-lapse-imaging-of-cleavage-divisions-in-embryo-quality-assessment
#18
REVIEW
Robert Milewski, Anna Ajduk
In vitro fertilization (IVF) is one of the most important procedures for treating infertility. As several embryos are usually produced in a single IVF cycle, it is crucial to select only the most viable ones for transfer to the patient. Morphokinetics, i.e. analysis of the dynamics of cleavage divisions and processes such as compaction and cavitation, has provided both biologists and clinicians with a new set of data regarding embryonic behaviour during preimplantation development and its association with embryo quality...
August 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28384614/how-much-information-about-embryo-implantation-potential-is-included-in-morphokinetic-data-a-prediction-model-based-on-artificial-neural-networks-and-principal-component-analysis
#19
Robert Milewski, Agnieszka Kuczyńska, Bożena Stankiewicz, Waldemar Kuczyński
PURPOSE: The aim of this study was to answer the question of how much information about embryo implantation potential can be obtained from morphokinetic parameters through the creation a predictive model based on morphokinetic information and using advanced data-mining and artificial intelligence methods. MATERIALS AND METHODS: Time-lapse recordings of 610 embryos were included in the analysis. For each embryo, absolute (t2, t3, t4, t5) and relative (cc2 and s2) morphokinetic parameters were collected...
March 2017: Advances in Medical Sciences
https://www.readbyqxmd.com/read/28264747/oocyte-morphology-and-embryo-morphokinetics-in-an-intra-cytoplasmic-sperm-injection-programme-is-there-a-relationship
#20
Azita Faramarzi, Mohammad Ali Khalili, Sareh Ashourzadeh
The aim was to investigate the relationship between the morphological parameters of metaphase II (MII) oocytes with morphokinetic variables of embryos following an intra-cytoplasmic sperm injection (ICSI) procedure. Morphokinetic behaviour and abnormal cleavage patterns of 334 zygotes were analyzed using time-lapse monitoring (TLM). In addition, oocyte morphology was assessed in relation to embryo morphokinetic (absolute time point, including time to second polar body (PB) extrusion (ESPB), pronuclei (PN) appearance (PNA), PN fading (PNF), time to 2-cells (t2), 3c (t3), 4c (t4), 5c (t5), 6c (t6), 7c (t7), 8c (t8) and relative timing parameters (S1, S2, CC2 and CC3)...
April 2017: Zygote: the Biology of Gametes and Early Embryos
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