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Lessly P Sepulveda-Rincon, Delphine Dube, Pierre Adenot, Ludivine Laffont, Sylvie Ruffini, Laurence Gall, Bruce K Campbell, Veronique Duranthon, Nathalie Beaujean, Walid E Maalouf
The first lineage specification during mammalian embryo development can be visually distinguished at blastocyst stage. Two cell lineages are observed on the embryonic/abembryonic axis of the blastocyst: the inner cell mass and the trophectoderm. The timing and mechanisms driving this process are still not fully understood. In mouse embryos, cells seem pre-patterned to become certain cell lineage; as the first cleavage plane has been related with further embryonic-abembryonic axis at blastocyst stage. Nevertheless, this possibility has been very debatable...
October 19, 2016: Biology of Reproduction
A A Mandawala, S C Harvey, T K Roy, K E Fowler
In vitro fertilisation is an effective method of assisted reproductive technology in both humans and certain non-human animal species. In most species, specifically, in humans and livestock, high in vitro fertilisation success rates are achieved via the transfer of embryos with the highest implantation and subsequent developmental potential. In order to reduce the risk of multiple gestation, which could be a result of the transfer of several embryos per cycle, restrictive transfer policies and methods to improve single embryo selection have been implemented...
September 29, 2016: Animal Reproduction Science
Liron Bar-El, Yael Kalma, Mira Malcov, Tamar Schwartz, Shaul Raviv, Tania Cohen, Hadar Amir, Yoni Cohen, Adi Reches, Ami Amit, Dalit Ben-Yosef
PURPOSE: The purpose of the study was to explore the effect of blastomere biopsy for preimplantation genetic diagnosis (PGD) on the embryos' dynamics, further cleavage, development, and implantation. METHODS: The study group included 366 embryos from all PGD treatments (September 2012 to June 2014) cultured in the EmbryoScope™ time-lapse monitoring system. The control group included all intracytoplasmic sperm injection (ICSI) embryos cultured in EmbryoScope™ until day 5 during the same time period (385 embryos)...
October 1, 2016: Journal of Assisted Reproduction and Genetics
Séverine Le Gac, Verena Nordhoff
The optimization of in-vitro culture conditions and the selection of the embryo(s) with the highest developmental competence are essential components in an ART program. Culture conditions are manifold and they underlie not always evidence-based research but also trends entering the IVF laboratory. At the moment, the idea of using sequential media according to the embryo requirements has been given up in favor of the use of single step media in an uninterrupted manner due to practical issues such as time-lapse incubators...
September 27, 2016: Molecular Human Reproduction
T T F Huang, K Chinn, T Kosasa, H J Ahn, B Kessel
Time-lapse imaging offers new tools to study dynamic processes of development such as blastocyst formation and expansion. This study quantitatively describes expansion in human blastocysts from donated oocytes. Measurements of hourly interval rate of changes in the blastocoel cross-sectional area revealed oscillatory pulses having 2-4 h periodicities. Two types of oscillations were distinguished. An E-Type ('expansion') had positive peak and positive or slightly negative trough interval rate of change values, and these characterized most of the expansion period...
September 12, 2016: Reproductive Biomedicine Online
George Α Sigalos, Olga Triantafyllidou, Nikos F Vlahos
PURPOSE: The final success of an IVF attempt depends on several steps and decisions taken during the ovarian stimulation, the oocyte retrieval, the embryo culture and the embryo transfer. The final selection of the embryos most likely to implant is the final step in this process and the responsibility of the lab. Apart from strict morphologic criteria that historically have been used in embryo selection, additional information on genetic, metabolomic and morphokinetic characteristics of the embryo is recently combined to morphology to select the embryo most likely to produce a pregnancy...
September 15, 2016: Archives of Gynecology and Obstetrics
Bjørn Molt Petersen, Mikkel Boel, Markus Montag, David K Gardner
STUDY QUESTION: Can a generally applicable morphokinetic algorithm suitable for Day 3 transfers of time-lapse monitored embryos originating from different culture conditions and fertilization methods be developed for the purpose of supporting the embryologist's decision on which embryo to transfer back to the patient in assisted reproduction? SUMMARY ANSWER: The algorithm presented here can be used independently of culture conditions and fertilization method and provides predictive power not surpassed by other published algorithms for ranking embryos according to their blastocyst formation potential...
October 2016: Human Reproduction
Maria Giulia Minasi, Alessandro Colasante, Teresa Riccio, Alessandra Ruberti, Valentina Casciani, Filomena Scarselli, Francesca Spinella, Francesco Fiorentino, Maria Teresa Varricchio, Ermanno Greco
STUDY QUESTION: Are there correlations among human blastocyst ploidy status, standard morphology evaluation and time-lapse kinetics? SUMMARY ANSWER: Correlations were observed, in that euploid human blastocysts showed a higher percentage with top quality inner cell mass (ICM) and trophectoderm (TE), higher expansion grades and shorter time to start of blastulation, expansion and hatching, compared to aneuploid ones. WHAT IS KNOWN ALREADY: Embryo quality has always been considered an important predictor of successful implantation and pregnancy...
October 2016: Human Reproduction
P Drábková, L Andrlová, R Hampl, R Kanďár
The aim of this study was to find some relationship between amino acid metabolism and the embryo morphokinetic parameters studied via time-lapse analysis. Study included 48 human embryo samples and their culture media. Two groups of embryos were identified: embryos reached the 8-cell stage on day 3 (n=34) and embryos failed to develop at any point during the incubation (n=14). Amino acids levels were measured on day 3 of embryo development; using time-lapse analysis, the precise timing of embryo cleavage, synchrony of division, grade of fragmentation etc...
July 15, 2016: Physiological Research
Deven V Patel, Preeti B Shah, Aditi P Kotdawala, Javier Herrero, Irene Rubio, Manish R Banker
BACKGROUND: Embryonic aneuploidy may result in miscarriage, implantation failure, or birth defects. Thus, it is clinically necessary to avoid the selection of aneuploid embryos during in vitro fertilization treatment. AIM: The aim of this study was to identify the morphokinetic differences by analyzing the development of euploid and aneuploid embryos using a time-lapse technology. We also checked the accuracy of a previously described model for selection of euploid embryos based on morphokinetics in our study population...
April 2016: Journal of Human Reproductive Sciences
T Silva, E C Santos, K Annes, C A Soares, R F Leite, C B Lima, M P Milazzotto
The kinetics of in vitro-produced (IVP) bovine embryos is related to embryo viability, metabolism, and epigenetic patterns. Therefore, we believe that embryos with different speeds of development also respond differently to stress. In the present study, we performed global metabolic analysis (matrix-assisted laser desorption ionization time of flight mass spectrometry [MALDI-TOF]) of culture media, characterized apoptotic events (Terminal deoxynucleotidyl transferase dUTP nick end labeling [TUNEL] and caspase quantitation), and quantified transcript abundance of stress-related gene (real-time quantitative polymerase chain reaction [qRT-PCR]) in IVP bovine embryos with different developmental kinetics to investigate possible markers of stress response...
September 15, 2016: Theriogenology
Hanna Balakier, Agata Sojecki, Gelareh Motamedi, Clifford Librach
OBJECTIVE: To study the effect of human embryo multinucleation on the rate of aneuploidy, in vitro developmental morphokinetics, and pregnancy outcome. DESIGN: Retrospective study. SETTING: University-affiliated fertility center. PATIENT(S): A total of 296 patients undergoing IVF cycles. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Rate of multinucleation at the 2- and 4-cell stage, time-lapse morphokinetic parameters from zygote to blastocyst stage, ploidy of embryos analyzed by means of trophectoderm biopsy and array comparative genomic hybridization (PGS), and pregnancy outcome...
September 1, 2016: Fertility and Sterility
Thi T Truong, Yu May Soh, David K Gardner
STUDY QUESTION: What is the effect of three antioxidants (acetyl-L-carnitine, N-acetyl-L-cysteine and α-lipoic acid), when used individually and in combination, on mouse embryo development in culture, and subsequent fetal development post-transfer? SUMMARY ANSWER: A combination of antioxidants resulted in significant increases in blastocyst cell number, maintained intracellular glutathione (GSH) levels, supported earlier cleavage times from 5-cell stage to expanded blastocyst, and improved fetal developmental irrespective of incubator oxygen concentration...
July 2016: Human Reproduction
Aisling Ahlstrom, Hannah Park, Christina Bergh, Ulrika Selleskog, Kersti Lundin
Numerous studies have reported on the potential value of time-lapse variables for prediction of embryo viability. However, these variables have not been evaluated in combination with conventional morphological grading and patient characteristics. The aim of this study was to assess the ability of patient characteristics and embryo morphology together with morphokinetic variables to predict live birth after day 2 transfer. This retrospective analysis included 207 transferred embryos from 199 couples cultured in a time-lapse system up to day 2 of development...
July 2016: Reproductive Biomedicine Online
Xiangyi Kong, Shuting Yang, Fei Gong, Changfu Lu, Shuoping Zhang, Guangxiu Lu, Ge Lin
Day 3 cleavage embryo transfer is routine in many assisted reproductive technology centers today. Embryos are usually selected according to cell number, cell symmetry and fragmentation for transfer. Many studies have showed the relationship between cell number and embryo developmental potential. However, there is limited understanding of embryo division behavior and their association with embryo cell number and developmental potential. A retrospective and observational study was conducted to investigate how different division behaviors affect cell number and developmental potential of day 3 embryos by time-lapse imaging...
2016: PloS One
Ali Sami Gurbuz, Funda Gode, Mehmet Sukru Uzman, Betul Ince, Melek Kaya, Necati Ozcimen, Emel Ebru Ozcimen, Ali Acar
BACKGROUND: To evaluate the effects of an ovulation triggering agent, human chorionic gonadotropin (hCG), versus a gonadotropin-releasing hormone agonist (GnRHa) on early embryo development in vitro using a time-lapse system. METHODS: Retrospective analysis of a prospectively collected database. A total of 739 embryos from 152 infertile couples undergoing intracytoplasmic sperm injection cycles. INTERVENTIONS: Embryo culture in a time-lapse incubator (EmbryoScope, Vitrolife, Göteborg, Sweden)...
2016: Journal of Ovarian Research
Jesus Aguilar, Irene Rubio, Elkin Muñoz, Antonio Pellicer, Marcos Meseguer
OBJECTIVE: To study nucleation status in two- and four-cell embryos and its effect on reproductive outcomes. DESIGN: Retrospective cohort study. SETTING: University-affiliated private center. PATIENT(S): A total of 1,679 embryos from 940 oocyte donation cycles from May 2012 to May 2014. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Implantation, morphokinetics, and nucleation status restoration...
August 2016: Fertility and Sterility
Alberto Tejera, Damia Castelló, Jose Maria de Los Santos, Antonio Pellicer, Jose Remohí, Marcos Meseguer
OBJECTIVE: To evaluate correlations between oxygen consumption (OC) measurements before and after embryo cytokinesis, observing OC during embryo cleavages and combining that information with morphokinetics to relate to implantation potential. DESIGN: Prospective cohort study. SETTING: University-affiliated private IVF unit. PATIENT(S): A total of 1,150 injected oocytes in 86 first oocyte donation cycles with embryo transfer on day 3...
July 2016: Fertility and Sterility
Karin E Lindgren, Julius Hreinsson, Malin Helmestam, Kjell Wånggren, Inger Sundström Poromaa, Karin Kårehed, Helena Åkerud
UNLABELLED: Histidine-rich glycoprotein (HRG) is an abundant plasma protein involved in multiple biological processes including immunology, vascularisation, and coagulation. These processes are of importance in regulating embryo development and implantation. A specific polymorphism in the HRG gene, HRG C633T, has an impact on various aspects of fertility, such as oocyte quality, endometrial receptivity, and possibly the capacity of the embryo itself to implant. To further examine the potential role of the HRG C633T polymorphism in regulating endometrial angiogenesis and on embryo development, two HRG peptides were constructed...
June 2016: Systems Biology in Reproductive Medicine
Daniel Bodri, Takeshi Sugimoto, Jazmina Yao Serna, Satoshi Kawachiya, Ryutaro Kato, Tsunekazu Matsumoto
OBJECTIVE: To ascertain the rate of blastocyst collapse observed by time-lapse monitoring in a retrospective cohort of unselected infertile patients undergoing single blastocyst transfer and to determine its association with live birth. DESIGN: Blastocyst collapse and morphokinetic variables were scored according to previously published criteria. The association between blastocyst collapse and live birth was evaluated by a multivariate logistic regression analysis including morphokinetic variables and other confounders...
June 2016: Fertility and Sterility
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