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https://www.readbyqxmd.com/read/29664791/embryo-morphokinetic-characteristics-and-euploidy
#1
Francesca Pennetta, Cristina Lagalla, Andrea Borini
PURPOSE OF REVIEW: The purpose of the current review is to provide an update on time-lapse morphokinetic assessment related to embryo ploidy status. RECENT FINDINGS: The main limitation of the available studies regarding correlation between morphokinetic variables and ploidy is that each embryo is considered as an independent unit whereas recent findings show that embryo kinetics may be affected by patient and ovarian stimulation-related factors, so that clustered data analysis is more appropriate...
June 2018: Current Opinion in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29662827/retrospective-study-of-single-vitrified-warmed-blastocyst-transfer-cycles-according-to-the-presence-of-morphokinetic-variables
#2
Yong Soo Hur, Eun Kyung Ryu, Chang Seop Hyun, Seong Ho Yang, San Hyun Yoon, Kyung Sil Lim, Won Don Lee, Jin Ho Lim
This study retrospectively assessed whether time-lapse data relating to developmental timing and morphology were associated with clinical outcomes, with the eventual goal of using morphokinetic variables to select embryos prospectively for cryopreservation. In this study, we examined the clinical outcomes of single vitrified-warmed blastocyst transfer cycles that were cultured in a time-lapse incubation system. The morphokinetic variables included uneven pronuclei, an uneven blastomere, multinucleation, and direct, rapid, and irregular division...
March 2018: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29551013/the-interval-transfer-of-a-frozen-thawed-embryo-is-more-successful-than-a-fresh-embryo-transfer-for-women-undergoing-ivf-with-recurrent-implantation-failure-after-cleavage-stage-embryo-biopsy
#3
Jennifer C Pontré, John P Ryan, Andy Tan, Roger J Hart
BACKGROUND: Recurrent implantation failure (RIF) is repeated unsuccessful embryo transfers (ETs). AIMS: To identify predictive embryonic markers of implantation in RIF, following pre-implantation genetic screening (PGS) of cleavage stage embryos, after accounting for male and female factors. MATERIALS AND METHODS: Retrospective analysis of RIF patients undergoing PGS after correction of modifiable causes. RESULTS: Eighty-four patients underwent 140 in vitro ferilisation cycles...
March 18, 2018: Australian & New Zealand Journal of Obstetrics & Gynaecology
https://www.readbyqxmd.com/read/29527703/is-there-any-correlation-between-sperm-parameters-and-chromatin-quality-with-embryo-morphokinetics-in-patients-with-male-infertility
#4
E Mangoli, M A Khalili, A R Talebi, S Ghasemi-Esmailabad, A Hosseini
The main goal was to evaluate the correlation between sperm parameters and chromatin quality with embryo kinetics via time-lapse monitoring system (TLM). A total of 40 couples involved in the ICSI program as a result of male infertility. For assessment of sperm chromatin and DNA quality, we used aniline blue, toluidine blue, chromomycin A3, acridine orange and terminal transferase-mediated deoxyuridine triphosphate biotin end labelling assays. All mature oocytes were injected, and the generated zygotes (2PNs) were cultured in TLM...
March 12, 2018: Andrologia
https://www.readbyqxmd.com/read/29458909/effect-of-morphokinetics-and-morphological-dynamics-of-cleavage-stage-on-embryo-developmental-potential-a-time-lapse-study
#5
Shiao-Hsuan Yang, Cheng-Hsuan Wu, Yu-Ching Chen, Chueh-Ko Yang, Tsui-Huang Wu, Pei-Chi Chen, Horng-Der Tsai
OBJECTIVE: Using a non-invasive method to select the most competent embryo is essential in in vitro fertilization (IVF). Since the beginning of clinical application of time-lapse technology, several studies have proposed models using the time-lapse imaging system for predicting the IVF outcome. This study used both morphokinetic and morphological dynamic parameters to select embryos with the highest developmental potential. MATERIALS AND METHODS: A total of 23 intracytoplasmic sperm injection treatment cycles with 138 fertilized oocytes were included in this study...
February 2018: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29452698/are-cleavage-anomalies-multinucleation-or-specific-cell-cycle-kinetics-observed-with-time-lapse-imaging-predictive-of-embryo-developmental-capacity-or-ploidy
#6
Nina Desai, Jeffrey M Goldberg, Cynthia Austin, Tommaso Falcone
OBJECTIVE: To determine whether cleavage anomalies, multinucleation, and specific cellular kinetic parameters available from time-lapse imaging are predictive of developmental capacity or blastocyst chromosomal status. DESIGN: Retrospective analysis of prospectively collected data. SETTING: Single academic center. PATIENT(S): A total of 1,478 zygotes from patients with blastocysts biopsied for preimplantation genetic screening were cultured in the EmbryoScope...
April 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29428076/an-automated-blastomere-identification-method-for-the-evaluation-of-day-2-embryos-during-ivf-icsi-treatments
#7
Charalambos Strouthopoulos, George Anifandis
PURPOSE: Evaluation of human embryos is one of the most important challenges in vitro fertilization (IVF) programs. The morphology and the morphokinetic parameters of the early cleaving embryo are of critical clinical importance. This stage spans the first 48 h post-fertilization, in which the embryo is dividing in smaller blastomeres at specific time-points. The morphology, in combination with the symmetry of the blastomeres seems to be powerful features with strong prognostic value for embryo evaluation...
March 2018: Computer Methods and Programs in Biomedicine
https://www.readbyqxmd.com/read/29398421/can-time-lapse-parameters-predict-embryo-ploidy-a-systematic-review
#8
REVIEW
Arnaud Reignier, Jenna Lammers, Paul Barriere, Thomas Freour
Embryo morphology assessment performs relatively poorly in predicting implantation. Embryo aneuploidy screening (PGS) has recently improved, but its clinical value is still debated, and the development of a cheap non-invasive method for the assessment of embryo ploidy status is a highly desirable goal. The growing implementation of time-lapse devices led some teams to test the effectiveness of morphokinetic parameters as predictors of embryo ploidy, with conflicting results. The aim of this study was to conduct a comprehensive review of the literature on the predictive value of morphokinetic parameters for embryo ploidy status...
April 2018: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/29391166/a-speculative-outlook-on-embryonic-aneuploidy-can-molecular-pathways-be-involved
#9
REVIEW
Olga Tšuiko, Tatjana Jatsenko, Lalit Kumar Parameswaran Grace, Ants Kurg, Joris Robert Vermeesch, Fredrik Lanner, Signe Altmäe, Andres Salumets
The journey of embryonic development starts at oocyte fertilization, which triggers a complex cascade of events and cellular pathways that guide early embryogenesis. Recent technological advances have greatly expanded our knowledge of cleavage-stage embryo development, which is characterized by an increased rate of whole-chromosome losses and gains, mixoploidy, and atypical cleavage morphokinetics. Embryonic aneuploidy significantly contributes to implantation failure, spontaneous miscarriage, stillbirth or congenital birth defects in both natural and assisted human reproduction...
January 31, 2018: Developmental Biology
https://www.readbyqxmd.com/read/29353449/blastulation-timing-is-associated-with-differential-mitochondrial-content-in-euploid-embryos
#10
Jacqueline R Ho, Nabil Arrach, Katherine Rhodes-Long, Wael Salem, Lynda K McGinnis, Karine Chung, Kristin A Bendikson, Richard J Paulson, Ali Ahmady
PURPOSE: Preimplantation genetic screening (PGS) and assessment of mitochondrial content (MC) are current methods for selection of the best embryos for transfer. Studies suggest that time-lapse morphokinetics (TLM) may also be helpful for selecting embryos more likely to implant. In our study, we sought to examine the relationship between TLM parameters and MC to determine if they could be used adjunctively in embryo selection. We also examined the relationship between MC with ploidy and blastulation...
January 20, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29305546/multicenter-evaluation-of-the-accelerate-phenotest-bc-kit-for-rapid-identification-and-phenotypic-antimicrobial-susceptibility-testing-using-morphokinetic-cellular-analysis
#11
Preeti Pancholi, Karen C Carroll, Blake W Buchan, Raymond C Chan, Neelam Dhiman, Bradley Ford, Paul A Granato, Amanda T Harrington, Diana R Hernandez, Romney M Humphries, Matthew R Jindra, Nathan A Ledeboer, Shelley A Miller, A Brian Mochon, Margie A Morgan, Robin Patel, Paul C Schreckenberger, Paul D Stamper, Patricia J Simner, Nancy E Tucci, Cynthia Zimmerman, Donna M Wolk
We describe results from a multicenter study evaluating the Accelerate Pheno system, a first of its kind diagnostic system that rapidly identifies common bloodstream pathogens from positive blood cultures within 90 min and determines bacterial phenotypic antimicrobial susceptibility testing (AST) results within ∼7 h. A combination of fresh clinical and seeded blood cultures were tested, and results from the Accelerate Pheno system were compared to Vitek 2 results for identification (ID) and broth microdilution or disk diffusion for AST...
April 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29172789/morphometric-analysis-of-human-oocytes-using-time-lapse-does-it-predict-embryo-developmental-outcomes
#12
Azita Faramarzi, Mohammad Ali Khalili, Marjan Omidi
The aim of this prospective study was to evaluate the relationship between morphometric parameters of metaphase II (MII) oocytes and the morphokinetic behaviour of subsequent embryos derived by intra-cytoplasmic sperm injection (ICSI). The association between oocyte morphometry: (whole oocyte), ooplasm, width of zona pellucida (ZP) and perivitelline space (PVS) and first polar body (PB) with embryo morphokinetic variables, including time of second PB extrusion (tPB2), pronuclei appearance (tPN), pronuclei fading (tPNf), formation of two to eight cells (t2 to t8) and irregular cleavage events [uneven at two cells stage, cell fusion (Fu) and trichomonas mitoses (TM)] were assessed...
November 24, 2017: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/29149327/focused-time-lapse-analysis-reveals-novel-aspects-of-human-fertilization-and-suggests-new-parameters-of-embryo-viability
#13
G Coticchio, M Mignini Renzini, P V Novara, M Lain, E De Ponti, D Turchi, R Fadini, M Dal Canto
STUDY QUESTION: Can focused application of time-lapse microscopy (TLM) lead to a more detailed map of the morphokinetics of human fertilization, revealing novel or neglected aspects of this process? SUMMARY ANSWER: Intensive harnessing of TLM reveals novel or previously poorly characterised phenomena of fertilization, such as a cytoplasmic wave (CW) preceding pronuclear formation and kinetics of pronuclear chromatin polarization, thereby suggesting novel non-invasive biomarkers of embryo quality...
January 1, 2018: Human Reproduction
https://www.readbyqxmd.com/read/29141509/relative-morphokinetics-assessed-by-time-lapse-imaging-are-altered-in-embryos-from-patients-with-endometriosis
#14
Alexander Freis, Jens Erik Dietrich, Moritz Binder, Verena Holschbach, Thomas Strowitzki, Ariane Germeyer
INTRODUCTION: Time-lapse technology allows almost continuous noninvasive assessment of embryonic development. It was shown previously that relative kinetics defining cleavage synchronicity are better predictors of blastocyst quality than absolute time points. This study aims to compare relative kinetics in embryos from patients with and without endometriosis. METHODS: Time-lapse data were collected retrospectively from 596 patients undergoing infertility treatment for in vitro fertilization from January 2011 to July 2016...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/29118168/use-of-the-accelerate-pheno-system-for-identification-and-antimicrobial-susceptibility-testing-of-pathogens-in-positive-blood-cultures-and-impact-on-time-to-results-and-workflow
#15
Angella Charnot-Katsikas, Vera Tesic, Nedra Love, Brandy Hill, Cindy Bethel, Sue Boonlayangoor, Kathleen G Beavis
The Accelerate Pheno system uses automated fluorescence in situ hybridization technology with morphokinetic cellular analysis to provide rapid species identification (ID) and antimicrobial susceptibility testing (AST) results for the most commonly identified organisms in bloodstream infections. The objective was to evaluate the accuracy and workflow of bacterial and yeast ID and bacterial AST using the Accelerate Pheno system in the clinical microbiology laboratory. The consecutive fresh blood cultures received in the laboratory were analyzed by the Accelerate Pheno system within 0 to 8 h of growth detection...
January 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29101997/assessment-of-embryo-morphology-and-developmental-dynamics-by-time-lapse-microscopy-is-there-a-relation-to-implantation-and-ploidy
#16
REVIEW
Nikica Zaninovic, Mohamad Irani, Marcos Meseguer
Time-lapse microscopy (TLM) is an exciting novel technology with great potential for enhancing embryo selection in the embryology laboratory. This non-invasive objective assessment of embryos has provided a new tool for predicting embryo development and implantation potential. TLM detects several morphological phenomena that are often missed with static observations using conventional incubators, such as irregular divisions, blastocyst collapse and re-expansion, timing of blastocoel appearance, and timing of formation and internalization of fragments...
November 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/29080967/is-mitochondrial-dna-quantitation-in-blastocyst-trophectoderm-cells-predictive-of-developmental-competence-and-outcome-in-clinical-ivf
#17
REVIEW
Manuel Viotti, Andrea R Victor, Christo G Zouves, Frank L Barnes
Behind every successful IVF embryo transfer, there is a great game of chance. Methods seeking to tilt the balance and increase the likelihood of implantation have been proposed and implemented with varying results, including embryo morphology, FISH-PGS, comprehensive chromosomal screening (CCS), morphokinetics, endometrial receptivity testing. It has been suggested that mitochondrial DNA (mtDNA) copy number could serve as a biomarker for embryo viability, but this concept was recently challenged. The world of IVF is left with unanswered questions: Why are there discrepancies in the reports? Should mtDNA copy number be considered to rank embryos for transfer? And in a broader sense, how well must a technique be validated before its implementation in the IVF clinic? Here, we explore these questions attempting to piece together the published data and suggest future directions to help unravel the subject matter...
December 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29034763/pronuclear-pattern-does-not-predict-morphokinetics-behavior-in-human-embryos
#18
Azita Faramarzi, Mohammad Ali Khalili, Marjan Omidi, Azam Agha-Rahimi, Fatemeh Taheri
The purpose was to investigate the correlation between pronuclei (PN) morphology and morphokinetic behaviors of derived embryos with time lapse monitoring (TLM) in assisted reproduction setting. Over time, PN morphology from PN appearance (PNA) to PN fading (PNF), PNF according to size, contact, number and position of nuclear precursor bodies (NPBs) within each PN and morphokinetics variables, including absolute time points, relative timing parameters, cleavage patterns and arrest rate, were evaluated using TLM...
March 2018: Gynecological Endocrinology
https://www.readbyqxmd.com/read/28993454/dynamics-of-cytoplasm-and-cleavage-divisions-correlates-with-preimplantation-embryo-development
#19
Robert Milewski, Marcin Szpila, Anna Ajduk
In vitro fertilization has become increasingly popular as an infertility treatment. In order to improve efficiency of this procedure, there is a strong need for a refinement of existing embryo assessment methods and development of novel, robust and non-invasive selection protocols. Studies conducted on animal models can be extremely helpful here, as they allow for more extensive research on the potential biomarkers of embryo quality. In the present paper, we subjected mouse embryos to non-invasive time-lapse imaging and combined the Particle Image Velocimetry analysis of cytoplasmic dynamics in freshly fertilized oocytes with the morphokinetic analysis of recordings covering 5 days of preimplantation development...
January 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28974230/effect-of-day-3-embryo-morphometrics-and-morphokinetics-on-survival-and-implantation-after-slow-freezing-thawing-and-after-vitrification-warming-a-retrospective-cohort-study
#20
Elia Fernandez Gallardo, Carl Spiessens, Thomas D'Hooghe, Sophie Debrock
BACKGROUND: Morphometric and morphokinetic evaluation of in vitro cultured human embryos allows evaluation without time restriction and reduces intra- and inter-observer variability. Even though these technologies have been reported to improve the quality of cleavage stage embryo evaluation during fresh culture, possible advantages in the evaluation of cryopreserved embryos have been scarcely explored. This study aims to compare morphometric and morphokinetic parameters between slow frozen and vitrified embryos and to determine their relationship to embryo survival and implantation rate (IR) after thawing/warming...
October 3, 2017: Reproductive Biology and Endocrinology: RB&E
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