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Protein chaperones

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https://www.readbyqxmd.com/read/29908126/transglutaminase-type-2-in-the-regulation-of-proteostasis
#1
Manuela D'Eletto, Federica Rossin, Olga Fedorova, Maria Grazia Farrace, Mauro Piacentini
The maintenance of protein homeostasis (proteostasis) is a fundamental aspect of cell physiology that is essential for the survival of organisms under a variety of environmental and/or intracellular stress conditions. Acute and/or persistent stress exceeding the capacity of the intracellular homeostatic systems results in protein aggregation and/or damaged organelles that leads to pathological cellular states often resulting in cell death. These events are continuously suppressed by a complex macromolecular machinery that uses different intracellular pathways to maintain the proteome integrity in the various subcellular compartments ensuring a healthy cellular life span...
June 1, 2018: Biological Chemistry
https://www.readbyqxmd.com/read/29908125/roles-of-the-nucleotide-exchange-factor-and-chaperone-hsp110-in-cellular-proteostasis-and-diseases-of-protein-misfolding
#2
Unekwu M Yakubu, Kevin A Morano
Cellular protein homeostasis (proteostasis) is maintained by a broad network of proteins involved in synthesis, folding, triage, repair and degradation. Chief among these are molecular chaperones and their cofactors that act as powerful protein remodelers. The growing realization that many human pathologies are fundamentally diseases of protein misfolding (proteopathies) has generated interest in understanding how the proteostasis network impacts onset and progression of these diseases. In this minireview, we highlight recent progress in understanding the enigmatic Hsp110 class of heat shock protein that acts as both a potent nucleotide exchange factor to regulate activity of the foldase Hsp70, and as a passive chaperone capable of recognizing and binding cellular substrates on its own, and its integration into the proteostasis network...
June 1, 2018: Biological Chemistry
https://www.readbyqxmd.com/read/29907832/functional-switching-of-ascorbate-peroxidase-2-of-rice-osapx2-between-peroxidase-and-molecular-chaperone
#3
Sung Hyun Hong, Bhumi Nath Tripathi, Moon-Soo Chung, Chuloh Cho, Sungbeom Lee, Jin-Hong Kim, Hyoung-Woo Bai, Hyeun-Jong Bae, Jae-Young Cho, Byung Yeoup Chung, Seung Sik Lee
Ascorbate peroxidase (APX) is a class I haem-containing peroxidase, which catalyses the conversion of H2 O2 to H2 O and O2 using ascorbate as the specific electron donor. APX plays a central role in the elimination of intracellular reactive oxygen species (ROS) and protects plants from the oxidative damage that can occur as a result of biotic and abiotic stresses. At present, the only known function of APX is as a peroxidase. However, in this study, we demonstrate that Oryza sativa APX2 also operates as a molecular chaperone in rice...
June 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29907803/effect-of-temperature-on-burkholderia-pseudomallei-growth-proteomic-changes-motility-and-resistance-to-stress-environments
#4
Suporn Paksanont, Kitisak Sintiprungrat, Thatcha Yimthin, Pornpan Pumirat, Sharon J Peacock, Narisara Chantratita
Burkholderia pseudomallei is a flagellated, gram-negative environmental bacterium that causes melioidosis, a severe infectious disease of humans and animals in tropical areas. We hypothesised that B. pseudomallei may undergo phenotypic adaptation in response to an increase in growth temperature. We analysed the growth curves of B. pseudomallei strain 153 cultured in Luria-Bertani broth at five different temperatures (25 °C-42 °C) and compared the proteomes of bacteria cultured at 37 °C and 42 °C...
June 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29907755/molecular-chaperoning-helps-safeguarding-mitochondrial-integrity-and-motor-functions-in-the-sahara-silver-ant-cataglyphis-bombycina
#5
Quentin Willot, Patrick Mardulyn, Matthieu Defrance, Cyril Gueydan, Serge Aron
The Sahara silver ant Cataglyphis bombycina is one of the world's most thermotolerant animals. Workers forage for heat-stricken arthropods during the hottest part of the day, when temperatures exceed 50 °C. However, the physiological adaptations needed to cope with such harsh conditions remain poorly studied in this desert species. Using transcriptomics, we screened for the most heat-responsive transcripts of C. bombycina with aim to better characterize the molecular mechanisms involved with macromolecular stability and cell survival to heat-stress...
June 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29906241/exploring-the-roles-of-substrate-binding-surface-of-the-chaperone-site-in-the-chaperone-activity-of-trigger-factor
#6
Dongjie Fan, Shunan Cao, Qiming Zhou, You Zhang, Lei Yue, Chang Han, Bo Yang, Yu Wang, Zhuo Ma, Lingxiang Zhu, Chuanpeng Liu
Trigger factor (TF) is a key component of the prokaryotic chaperone network, which is involved in many basic cellular processes, such as protein folding, protein trafficking, and ribosome assembly. The major chaperone site of TF has a cradle-like structure in which protein substrate may fold without interference from other proteins. Here, we investigated in vivo and in vitro the roles of hydrophobic and charged patches on the edge and interior of cradle during TF-assisted protein folding. Our results showed that most of the surface of the cradle was involved in TF-assisted protein folding, which was larger than found in early studies...
June 15, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29905868/casein-kinase-2-mediated-phosphorylation-of-spt6-modulates-histone-dynamics-and-regulates-spurious-transcription
#7
Emmanuelle Gouot, Wajid Bhat, Anne Rufiange, Eric Fournier, Eric Paquet, Amine Nourani
CK2 is an essential protein kinase implicated in various cellular processes. In this study, we address a potential role of this kinase in chromatin modulations associated with transcription. We found that CK2 depletion from yeast cells leads to replication-independent increase of histone H3K56 acetylation and global activation of H3 turnover in coding regions. This suggests a positive role of CK2 in maintenance/recycling of the histone H3/H4 tetramers during transcription. Interestingly, strand-specific RNA-seq analyses show that CK2 inhibits global cryptic promoters driving both sense and antisense transcription...
June 14, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29905843/srna-dependent-control-of-curli-biosynthesis-in-escherichia-coli-mcas-directs-endonucleolytic-cleavage-of-csgd-mrna
#8
Patrick Rosendahl Andreassen, Jens Sivkær Pettersen, Mateusz Szczerba, Poul Valentin-Hansen, Jakob Møller-Jensen, Mikkel Girke Jørgensen
Production of curli, extracellular protein structures important for Escherichia coli biofilm formation, is governed by a highly complex regulatory mechanism that integrates multiple environmental signals through the involvement of numerous proteins and small non-coding RNAs (sRNAs). No less than seven sRNAs (McaS, RprA, GcvB, RydC, RybB, OmrA and OmrB) are known to repress the expression of the curli activator CsgD. Many of the sRNAs repress CsgD production by binding to the csgD mRNA at sites far upstream of the ribosomal binding site...
June 14, 2018: Nucleic Acids Research
https://www.readbyqxmd.com/read/29905794/a-brief-overview-of-the-swi1-prion-swi
#9
Dustin K Goncharoff, Zhiqiang Du, Liming Li
Prion and prion-like phenomena are involved in the pathology of numerous human neurodegenerative diseases. The budding yeast, Saccharomyces cerevisiae, has a number of endogenous yeast prions-epigenetic elements that are transmitted as altered protein conformations and often manifested as heritable phenotypic traits. One such yeast prion, [SWI+], was discovered and characterized by our laboratory. The protein determinant of [SWI+], Swi1 was found to contain an amino-terminal, asparagine-rich prion domain. Normally, Swi1 functions as part of the SWI/SNF chromatin remodeling complex, thus, acting as a global transcriptional regulator...
September 1, 2018: FEMS Yeast Research
https://www.readbyqxmd.com/read/29904335/neuroprotection-targeting-protein-misfolding-on-chronic-cerebral-hypoperfusion-in-the-context-of-metabolic-syndrome
#10
REVIEW
María I Herrera, Lucas D Udovin, Nicolás Toro-Urrego, Carlos F Kusnier, Juan P Luaces, Matilde Otero-Losada, Francisco Capani
Metabolic syndrome (MetS) is a cluster of risk factors that lead to microvascular dysfunction and chronic cerebral hypoperfusion (CCH). Long-standing reduction in oxygen and energy supply leads to brain hypoxia and protein misfolding, thereby linking CCH to Alzheimer's disease. Protein misfolding results in neurodegeneration as revealed by studying different experimental models of CCH. Regulating proteostasis network through pathways like the unfolded protein response (UPR), the ubiquitin-proteasome system (UPS), chaperone-mediated autophagy (CMA), and macroautophagy emerges as a novel target for neuroprotection...
2018: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/29904027/chaperonology-the-third-eye-on-brain-gliomas
#11
Francesca Graziano, C Caruso Bavisotto, A Marino Gammazza, Francesca Rappa, Everly Conway de Macario, Albert J L Macario, Francesco Cappello, Claudia Campanella, Rosario Maugeri, Domenico Gerardo Iacopino
The European Organization for Research and Treatment of Cancer/National Cancer Institute of Canada Phase III trial has validated as a current regimen for high-grade gliomas (HGG) a maximal safe surgical resection followed by radiotherapy with concurrent temozolamide. However, it is essential to balance maximal tumor resection with preservation of the patient’s neurological functions. Important developments in the fields of pre-operative and intra-operative neuro-imaging and neuro-monitoring have ameliorated the survival rate and the quality of life for patients affected by HGG...
June 14, 2018: Brain Sciences
https://www.readbyqxmd.com/read/29903771/daxx-functions-are-p53-independent-in-vivo
#12
Amanda R Wasylishen, Jeannelyn S Estrella, Vinod Pant, Gilda P Chau, Guillermina Lozano
Mutations in the death domain-associated protein (DAXX) have been recently identified in a substantial proportion of human pancreatic neuroendocrine tumors (PanNETs). Remarkably, however, little is known about the physiological role(s) of DAXX despite in vitro studies suggesting potential functions. Most prominently, and supported by tumor sequencing data, DAXX functions in concert with alpha thalassemia/mental retardation X-linked (ATRX) as a histone chaperone complex for the H3.3 variant. Studies have also identified potential roles in apoptosis, transcription, and negative regulation of the p53 tumor suppressor pathway...
June 14, 2018: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/29901626/curcumin-induces-endoplasmic-reticulum-stress-associated-apoptosis-in-human-papillary-thyroid-carcinoma-bcpap-cells-via-disruption-of-intracellular-calcium-homeostasis
#13
Li Zhang, Xian Cheng, Shichen Xu, Jiandong Bao, Huixin Yu
BACKGROUND: Thyroid cancer is the most common endocrine tumor. Our previous studies have demonstrated that curcumin can induce apoptosis in human papillary thyroid carcinoma BCPAP cells. However, the underlined mechanism has not been clearly elucidated. Endoplasmic reticulum (ER) is a major organelle for synthesis, maturation, and folding proteins as well as a large store for Ca. Overcoming chronically activated ER stress by triggering pro-apoptotic pathways of the unfolded protein response (UPR) is a novel strategy for cancer therapeutics...
June 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29899847/heat-shock-protein-90-inhibitors-augment-endogenous-wild-type-p53-expression-but-down-regulate-the-adenovirally-induced-expression-by-inhibiting-a-proteasome-activity
#14
Kuan Chai, Xuerao Ning, Thảo Thi Thanh Nguyễn, Boya Zhong, Takao Morinaga, Zhihan Li, Masato Shingyoji, Yuji Tada, Koichiro Tatsumi, Hideaki Shimada, Kenzo Hiroshima, Naoto Yamaguchi, Masatoshi Tagawa
Heat shock protein 90 (HSP90) inhibitors suppressed MDM4 functions which mediated p53 ubiquitination, and blocked a chaperon function which influenced expression of the client proteins. We examined cytotoxic effects of the inhibitors, 17-allylamino-17-demetheoxygeldanamycin (17-AAG) and 17-dimethylaminoethylamino-17-demethoxy-geldanamycin (17-DMAG), on mesothelioma and investigated combinatory effects of the inhibitors and adenoviruses expressing the wild-type p53 gene (Ad-p53). A majority of mesothelioma lacks p14 and p16 expression, which leads to defective p53 pathway despite bearing the wild-type p53 genotype...
May 25, 2018: Oncotarget
https://www.readbyqxmd.com/read/29899117/ccp1-modulates-epigenetic-stability-at-centromeres-and-affects-heterochromatin-distribution-in-schizosaccharomyces-pombe
#15
Min Lu, Xiangwei He
Distinct chromatin organization features, such as centromeres and heterochromatin domains, are inherited epigenetically. However, the mechanisms that modulate the accuracy of epigenetic inheritance, especially at the individual nucleosome level, are not well understood. Here, using ChIP and next-generation sequencing (ChIP-Seq), we characterized Ccp1, a homolog of the histone chaperone Vps75 in budding yeast that functions in centromere chromatin duplication and heterochromatin maintenance in fission yeast (Schizosaccharomyces pombe)...
June 13, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29898402/different-material-states-of-pub1-condensates-define-distinct-modes-of-stress-adaptation-and-recovery
#16
Sonja Kroschwald, Matthias C Munder, Shovamayee Maharana, Titus M Franzmann, Doris Richter, Martine Ruer, Anthony A Hyman, Simon Alberti
How cells adapt to varying environmental conditions is largely unknown. Here, we show that, in budding yeast, the RNA-binding and stress granule protein Pub1 has an intrinsic property to form condensates upon starvation or heat stress and that condensate formation is associated with cell-cycle arrest. Release from arrest coincides with condensate dissolution, which takes minutes (starvation) or hours (heat shock). In vitro reconstitution reveals that the different dissolution rates of starvation- and heat-induced condensates are due to their different material properties: starvation-induced Pub1 condensates form by liquid-liquid demixing and subsequently convert into reversible gel-like particles; heat-induced condensates are more solid-like and require chaperones for disaggregation...
June 12, 2018: Cell Reports
https://www.readbyqxmd.com/read/29895209/endoplasmic-reticulum-stress-in-ischemic-and-nephrotoxic-acute-kidney-injury
#17
Mingjuan Yan, Shaoqun Shu, Chunyuan Guo, Chengyuan Tang, Zheng Dong
Acute kidney injury is a medical condition characterized by kidney damage with a rapid decline of renal function, which is associated with high mortality and morbidity. Recent research has further established an intimate relationship between acute kidney injury and chronic kidney disease. Perturbations of kidney cells in acute kidney injury result in the accumulation of unfolded and misfolded proteins in the endoplasmic reticulum, leading to unfolded protein response or endoplasmic reticulum stress. In this review, we analyze the role and regulation of endoplasmic reticulum stress in acute kidney injury triggered by renal ischemia-reperfusion and cisplatin nephrotoxicity...
June 12, 2018: Annals of Medicine
https://www.readbyqxmd.com/read/29895095/valosin-containing-protein-vcp-p97-is-essential-for-the-intracellular-development-of-leishmania-and-its-survival-under-heat-stress
#18
Bruno G Aguiar, Prasad K Padmanabhan, Carole Dumas, Barbara Papadopoulou
VCP/p97/Cdc48 is one of the best-characterized type II cytosolic AAA+ ATPases most known for their role in ubiquitin-dependent protein quality control. Here, we provide functional insights into the role of the Leishmania VCP/p97 homolog (LiVCP) in the parasite intracellular development. We demonstrate that although LiVCP is an essential gene, L. infantum promastigotes can grow with less VCP. In contrast, growth of axenic and intracellular amastigotes is dramatically affected upon decreased LiVCP levels in heterozygous and temperature sensitive LiVCP mutants or the expression of dominant negative mutants known to specifically target the second conserved VCP ATPase domain, a major contributor of the VCP overall ATPase activity...
June 12, 2018: Cellular Microbiology
https://www.readbyqxmd.com/read/29894805/expression-and-purification-of-human-phosphatase-and-actin-regulator-1-phactr1-in-plant-based-systems
#19
B B Gengenbach, C R Müschen, J F Buyel
Cardiovascular diseases are a prevalent cause of morbidity and mortality especially in industrialized countries. The human phosphatase and actin regulator 1 (PHACTR1) may be involved in such diseases, but its precise regulatory function remains unclear due to the large number of potential interaction partners. The same phenomenon makes this protein difficult to express in mammalian cells, but it is also an intrinsically disordered protein that likely aggregates when expressed in bacteria due to the absence of chaperones...
June 9, 2018: Protein Expression and Purification
https://www.readbyqxmd.com/read/29894802/increased-soluble-heterologous-expression-of-a-rat-brain-3-o-sulfotransferase-1-a-key-enzyme-for-heparin-biosynthesis
#20
Weihua Jin, Shuai Li, Jiale Chen, Bing Liu, Jie Li, Xueliang Li, Fuming Zhang, Robert J Linhardt, Weihong Zhong
Heparan sulfate (HS), is a glycosaminoglycan (GAG) involved in various biological processes, including blood coagulation, wound healing and embryonic development. HS 3-O-sulfotransferases (3-OST), which transfer the sulfo group to the 3-hydroxyl group of certain glucosamine residues, is a key enzyme in the biosynthesis of a number of biologically important HS chains. The 3-OST-1 isoform is one of the 7 known 3-OST isoforms and is important for the biosynthesis of anticoagulant HS chains. In this study, we cloned 3-OST-1 from the rat brain by reverse transcription-polymerase chain reaction (RT-PCR)...
June 9, 2018: Protein Expression and Purification
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