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https://www.readbyqxmd.com/read/29244272/comparison-of-perioperative-outcomes-with-or-without-renorrhaphy-during-open-partial-nephrectomy-a-propensity-score-matched-analysis
#1
Hidekazu Tachibana, Toshio Takagi, Tsunenori Kondo, Hideki Ishida, Kazunari Tanabe
PURPOSE: Renorrhaphy in partial nephrectomy may damage intraparenchymal vessels and compress the renal parenchyma, which may lead to the formation of renal artery pseudoaneurysms or vascularized parenchymal volume reduction. Using propensity score matching, we compared surgical outcomes following non-renorrhaphy and renorrhaphy techniques for open partial nephrectomy (OPN) for T1a renal tumors. MATERIALS AND METHODS: We retrospectively analyzed data from 159 patients with normal contralateral kidneys who underwent OPN for T1a renal tumors and pre- and postoperative enhanced computed tomography between 2012 and 2015...
December 18, 2017: International Braz J Urol: Official Journal of the Brazilian Society of Urology
https://www.readbyqxmd.com/read/29236240/effects-of-small-molecules-water-that-may-retard-kidney-stone-formation
#2
Yang Li, Jichuan Pan, Yue Zhang, Yangtao Chang, Xiaoxiong Yang, Baoyu Yang, Xu Mao, Zhonghui Wang, Bing Gao, Xiuli Lu
PURPOSE: Water intake is important for preventing kidney stones. Small molecules water is a more active restructured water under magnetic field. Here, we studied the relation between small molecules water and crystal formation in the rat kidney. MATERIALS: The small molecules water was prepared by a water machine providing a 0.8-T magnetic field. Calcium oxalate crystals were induced by 0.75% EG (ethylene glycol) in male Sprague-Dawley rats by drinking small molecules water and plain water for up to 6 weeks, respectively...
December 13, 2017: International Urology and Nephrology
https://www.readbyqxmd.com/read/29236151/the-effects-of-hap-and-macrophage-cells-to-the-expression-of-inflammatory-factors-and-apoptosis-in-hk-2-cells-of-vitro-co-cultured-system
#3
Junchuan Yu, Yaoliang Deng, Zhiwei Tao, Weixia Liang, Xiaofeng Guan, Jihua Wu, Xin Ning, Yunlong Liu, Quan Liu, Ziqi He
This study developed an in vitro system by co-culturing HK-2 cells with different concentration of hydroxyapatite (HAP) and/or macrophage cells to simulate the internal environment of urolithiasis as far as possible, investigating the regulatory effects of macrophage cells on HAP-induced expression of relative inflammatory factors of HK-2 cells. The control group (H group) was only comprised of HK-2 cells. Experimental groups included co-culturing HK-2 cells and macrophage cells (H + M group), co-culturing HK-2 cells and HAP (H + A group), co-culturing macrophage cells and HAP (M + A group), and co-culturing HK-2 cells and macrophage cells with HAP (H + M + A group)...
December 13, 2017: Urolithiasis
https://www.readbyqxmd.com/read/29230389/cyclic-tensile-strain-can-play-a-role-in-directing-both-intramembranous-and-endochondral-ossification-of-mesenchymal-stem-cells
#4
Simon F Carroll, Conor T Buckley, Daniel J Kelly
Successfully regenerating damaged or diseased bone and other joint tissues will require a detailed understanding of how joint specific environmental cues regulate the fate of progenitor cells that are recruited or delivered to the site of injury. The goal of this study was to explore the role of cyclic tensile strain (CTS) in regulating the initiation of mesenchymal stem cell/multipotent stromal cell (MSC) differentiation, and specifically their progression along the endochondral pathway. To this end, we first explored the influence of CTS on the differentiation of MSCs in the absence of any specific growth factor, and secondly, we examined the influence of the long-term application of this mechanical stimulus on markers of endochondral ossification in MSCs maintained in chondrogenic culture conditions...
2017: Frontiers in Bioengineering and Biotechnology
https://www.readbyqxmd.com/read/29229947/recombinant-human-osteopontin-expressed-in-nicotiana-benthamiana-stimulates-osteogenesis-related-genes-in-human-periodontal-ligament-cells
#5
Kaewta Rattanapisit, Supaniga Abdulheem, Daneeya Chaikeawkaew, Anchanee Kubera, Hugh S Mason, Julian K-C Ma, Prasit Pavasant, Waranyoo Phoolcharoen
Tissue engineering aims to utilise biologic mediators to facilitate tissue regeneration. Several recombinant proteins have potential to mediate induction of bone production, however, the high production cost of mammalian cell expression impedes patient access to such treatments. The aim of this study is to produce recombinant human osteopontin (hOPN) in plants for inducing dental bone regeneration. The expression host was Nicotiana benthamiana using a geminiviral vector for transient expression. OPN expression was confirmed by Western blot and ELISA, and OPN was purified using Ni affinity chromatography...
December 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29228885/multiplex-analysis-of-serum-hormone-and-cytokine-in-patients-with-cervical-copll-towards-understanding-the-potential-pathogenic-mechanisms
#6
Guo-Dong Cai, Zhuang-Chen Zhu, Jun-Qin Wang, Guang Chen, Zeng Wang, Chen-Song Yang, Gui-Xin Sun
Cervical ossification of the posterior longitudinal ligament (cOPLL) is one of the major causes of myelopathy. However, the mechanism underlying remains elusive. In the present study, using MILLIPLEX magnetic bead panel, we investigated four serum hormones and six serum cytokines in cOPLL patients and healthy subjects. The results showed that tumor necrosis factore-α (TNF-α) were significantly increased, and DDK-1 was significantly decreased in the serum from male and female cOPLL patients compared with those from healthy controls, respectively...
October 2017: Growth Factors
https://www.readbyqxmd.com/read/29228698/osteopontin-is-involved-in-tlr4-pathway-contributing-to-ovarian-cancer-cell-proliferation-and-metastasis
#7
Cong Xu, Hua Li, Miao Yin, Tao Yang, Liguo An, Guiwen Yang
Tumor cell proliferation and metastasis are critical for tumor progression and lead to death of cancer patients. TLR4 is a member of the toll-like receptor (TLR) family, which promotes tumor growth, metastasis and immune escape. Osteopontin (OPN), a phosphorylated glycoprotein extensively expressed in multiple cell-types, plays important roles in tumorigenesis, metastasis and infiltration, and participates in signal transduction of innate immunity. However, it is unclear whether TLR4 has any relationship with OPN...
November 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29223135/simultaneous-anti-diabetic-and-anti-vascular-calcification-activity-of-nocardia-sp-utmc-751
#8
Fatemeh Salimi, Saeedeh Jafari-Nodooshan, Nazanin Zohourian, Sedighe Kolivand, Javad Hamedi
Alpha-amylase can act as a significant player in causing hyperglycemia, leading to protein glycation, which is the main complication in this condition, besides causing vascular calcification (VC), an important vascular failure caused due to thisIn order to find a natural source of the biocompounds with inhibitory effects on alpha-amylase,, fifteen fermentation broth extracts of actinobacteria (FBEA) (200 μg mL-1 ) have been screened. Finally, the effects of the most efficient FBE have been investigated on osteopontin (OPN, a VC marker) mRNA level in the vascular smooth muscle cells under the calcification conditions, and the chemical constituents of the most efficient FBE were analyzed using GC-MS analysis...
December 9, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29221960/when-everything-else-fails-high-pressure-balloon-for-undilatable-lesions
#9
REVIEW
Ioannis Felekos, Grigoris V Karamasis, Antonis N Pavlidis
Heavily calcified lesions are encountered with increasing frequency during percutaneous coronary interventions (PCI). This poses a significant problem as it often leads to suboptimal stent expansion, which in turn is a major cause for stent restenosis and thrombosis. Various technologies have been developed to tackle this. The high-pressure balloon (OPN) seems to be a promising adjunct in treating undilatable lesions. In this concise mini review we highlight the use of HPB and we present the current status of technology implemented in complex PCI cases...
November 10, 2017: Cardiovascular Revascularization Medicine: Including Molecular Interventions
https://www.readbyqxmd.com/read/29218725/characterization-of-retinal-ganglion-cell-horizontal-cell-and-amacrine-cell-types-expressing-the-neurotrophic-receptor-tyrosine-kinase-ret
#10
Nadia Parmhans, Szilard Sajgo, Jingwen Niu, Wenqin Luo, Tudor Constantin Badea
We report the retinal expression pattern of Ret, a receptor tyrosine kinase for the Glial Derived Neurotrophic Factor (GDNF) family ligands (GFLs), during development and in the adult mouse. Ret is initially expressed in Retinal Ganglion Cells (RGCs), followed by Horizontal Cells (HCs) and Amacrine Cells (ACs), beginning with the early stages of postmitotic development. Ret expression persists in all three classes of neurons in the adult. Using RNA sequencing, immunostaining and random sparse recombination, we show that Ret is expressed in at least three distinct types of ACs, and ten types of RGCs...
December 8, 2017: Journal of Comparative Neurology
https://www.readbyqxmd.com/read/29218094/mir-351-negatively-regulates-osteoblast-differentiation-of-mscs-induced-by-cholesten-3-one-through-targeting-vdr
#11
Qiuke Hou, Yongquan Huang, Yiwen Luo, Bin Wang, Yamei Liu, Rudong Deng, Saixia Zhang, Fengbin Liu, Dongfeng Chen
Our previous reports indicated that (+)-cholesten-3-one induces osteogenic differentiation of bone marrow mesenchymal stem cells (MSCs) by activating vitamin D receptor (VDR). However, whether and how miRNAs modulate osteogenic differentiation induced by (+)-cholesten-3-one have not been explored. In this study, miRNA array profiling and further validation by quantitative real-time PCR revealed that miR-351 was downregulated during (+)-cholesten-3-one-induced osteogenic differentiation of MSCs. Overexpression of miR-351 by miR-351 precursor transfection markedly inhibited the expression of osteoblast-specific genes, such as alkaline phosphatase (ALP), collagen type II, osteopontin (OPN), and runt-related transcription factor 2 (RUNX2), which consequently decreased a number of calcium mineralized nodules...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/29214666/low-level-ultrahigh-frequency-and-ultrashort-pulse-blue-laser-irradiation-enhances-osteoblast-extracellular-calcification-by-upregulating-proliferation-and-differentiation-via-transient-receptor-potential-vanilloid-1
#12
Risako Mikami, Koji Mizutani, Akira Aoki, Yukihiko Tamura, Kazuhiro Aoki, Yuichi Izumi
BACKGROUND AND OBJECTIVE: Low-level laser irradiation (LLLI) exerts various biostimulative effects, including promotion of wound healing and bone formation; however, few studies have examined biostimulation using blue lasers. The purpose of this study was to investigate the effects of low-level ultrahigh-frequency (UHF) and ultrashort-pulse (USP) blue laser irradiation on osteoblasts. STUDY DESIGN/ MATERIALS AND METHODS: The MC3T3-E1 osteoblast cell line was used in this study...
December 7, 2017: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/29214433/overexpression-of-osteopontin-promotes-cell-proliferation-and-migration-in-human-nasopharyngeal-carcinoma-and-is-associated-with-poor-prognosis
#13
Haimei Qin, Rong Wang, Guijiang Wei, Huaifei Wang, Guogang Pan, Rentong Hu, Yesheng Wei, Renguang Tang, Junli Wang
Nasopharyngeal carcinoma (NPC), a malignant tumor at the top and side of the nasopharyngeal cavity, highly occurs in the southern region of China. Cancer cell metastasis is one of the leading causes of death in NPC patients. Osteopontin (OPN), is a phosphorylated extracellular matrix protein with a variety of functions, was found to be overexpressed in many cancers. However, the expression and role of OPN in patients with NPC in Guangxi, China are unclear. Here, we observed that NPC patients had upregulated OPN at mRNA protein and levels...
December 6, 2017: European Archives of Oto-rhino-laryngology
https://www.readbyqxmd.com/read/29209142/clinical-and-in-vitro-analysis-of-osteopontin-as-a-prognostic-indicator-and-unveil-its-potential-downstream-targets-in-bladder-cancer
#14
Janet P C Wong, Ran Wei, Peng Lyu, Olivia L H Tong, Shu Dong Zhang, Qing Wen, Hiu Fung Yuen, Mohamed El-Tanani, Hang Fai Kwok
Osteopontin (OPN) plays an important role in cancer progression, however its prognostic significance and its downstream factors are largely elusive. In this study, we have shown that expression of OPN was significantly higher in bladder cancer specimens with higher T-stage or tumor grades. In addition, a high level of OPN was significantly associated with poorer survival in two independent bladder cancer patient cohorts totaling 389 bladder cancer patients with available survival data. We further identified Matrix metallopeptidase 9 (MMP9) and S100 calcium-binding protein A8 (S100A8) were both downstream factors for OPN in bladder cancer specimens and bladder cancer cell lines...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/29208448/investigation-of-tgf%C3%AE-r2-snp-rs4522809-osteopontin-tgf-%C3%AE-1-and-their-association-with-dilatative-pathology-of-ascending-thoracic-aorta
#15
Zivile Staneviciute, Ramune Sepetiene, Ingrida Grabauskyte, Vaiva Patamsyte, Vaiva Lesauskaite
BACKGROUND: Dilatative pathology of the ascending thoracic aorta (DPATA) is characterized by the aortic wall expansion more than 1.5 and could be accompanied by aortic wall rupture. Mutations of TGFBR2 gene demonstrated an association with syndromic DPATA and altered pathway of transforming growth factor beta (TGF-β). Elevated TGF-β1 level has been found in blood samples in DPATA group. Moreover, elevated osteopontin (OPN) level was associated with mutations of TGFBR2 gene. Based on recently published findings, we aimed to evaluate genotyping results of TGFBR2 rs4522809 and the association with circulating OPN and TGF-β1 concentrations within DPATA patients...
December 2, 2017: Cytokine
https://www.readbyqxmd.com/read/29199325/-effect-of-overexpression-of-transcription-factor-runx2-and-osterix-on-osteogenic-differentiation-of-endothelial-cells
#16
Guang-Zheng Yang, Wen-Jie Zhang, Xun Ding, Xiang-Kai Zhang, Xin-Quan Jiang, Zhi-Yuan Zhang
PURPOSE: To explore the effect of overexpression of Runx2 and Osterix (OSX) genes on osteogenic differentiation of human umbilical vein endothelial cells (HUVECs). METHODS: Overexpressed Runx2 and OSX lentiviral vectors were transfected into HUVECs respectively. The osteogenic potential of transfected cells was identified by alkaline phosphatase (ALP) staining and ALP activity. Furthermore, real time-PCR, Western blot and immunofluorescence staining were performed to detect the expression of osteogenic genes and proteins in HUVECs...
August 2017: Shanghai Kou Qiang Yi Xue, Shanghai Journal of Stomatology
https://www.readbyqxmd.com/read/29196165/osteopontin-promotes-collagen-i-synthesis-in-hepatic-stellate-cells-by-mirna-129-5p-inhibition
#17
Yinghua Chen, Yitao Ou, Jiale Dong, Guizhi Yang, Zhi Zeng, Ying Liu, Bing Liu, Weidong Li, Xiaoshun He, Tian Lan
Activation of hepatic stellate cells (HSCs) is an essential event in the initiation and progression of liver fibrosis. HSCs are believed to be the major source of collagen-producing myofibroblasts in fibrotic livers. A key feature in the pathogenesis of liver fibrosis is fibrillar Collagen I (Col 1) deposition. Osteopontin (OPN), an extracellular matrix (ECM) cytokine expressed in HSCs, could drive fibrogenesis by modulating the HSC pro-fibrogenic phenotype and Col 1 expression. Here, we aimed to investigate the molecular mechanism of OPN regulating the activation of HSCs...
November 28, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29188073/osteopontin-is-associated-with-disease-severity-and-antiphospholipid-syndrome-in-well-characterised-swedish-cases-of-sle
#18
Lina Wirestam, Martina Frodlund, Helena Enocsson, Thomas Skogh, Jonas Wetterö, Christopher Sjöwall
Objective: The variety of disease phenotypes among patients with SLE challenges the identification of new biomarkers reflecting disease activity and/or organ damage. Osteopontin (OPN) is an extracellular matrix protein with immunomodulating properties. Although raised levels have been reported, the pathogenic implications and clinical utility of OPN as a biomarker in SLE are far from clear. Thus, the aim of this study was to characterise OPN in SLE. Methods: Sera from 240 well-characterised adult SLE cases classified according to the American College of Rheumatology (ACR) and/or the Systemic Lupus International Collaborating Clinics (SLICC) criteria, and 240 population-based controls were immunoassayed for OPN...
2017: Lupus Science & Medicine
https://www.readbyqxmd.com/read/29181544/angiotensin-1-7-but-not-the-thrombin-cleaved-osteopontin-c-terminal-fragment-attenuates-osteopontin-mediated-macrophage-induced-endothelial-cell-inflammation
#19
Rachel Hamias, Assaf Rudich, George Greenberg, Gabriel Szendro, Talya Wolak
OBJECTIVE AND DESIGN: Evaluating the pro-/anti-inflammatory activity of the C-terminal cleavage product of osteopontin in comparison to angiotensin 1-7. MATERIAL AND SUBJECTS: Human coronary endothelial cells (hcEC) treated with conditioned media from human U937 macrophages. TREATMENT: Macrophages were (pre)treated with C-terminal, full-length or N-terminal osteopontin (OPN-C, OPN-FL, OPN-N, respectively), angiotensin II, angiotensin 1-7 or TNF-α...
November 27, 2017: Inflammation Research: Official Journal of the European Histamine Research Society ... [et Al.]
https://www.readbyqxmd.com/read/29180017/role-of-osteopontin-and-its-regulation-in-pancreatic-islet
#20
Mengyin Cai, Pradeep Bompada, Albert Salehi, Juan R Acosta, Rashmi B Prasad, David Atac, Markku Laakso, Leif Groop, Yang De Marinis
Osteopontin (OPN) is involved in various physiological processes and also implicated in multiple pathological states. It has been suggested that OPN may have a role in type 2 diabetes (T2D) by protecting pancreatic islets and interaction with incretins. However, the regulation and function of OPN in islets, especially in humans, remains largely unexplored. In this study, we performed our investigations on both diabetic mouse model SUR1-E1506K+/+ and islets from human donors. We demonstrated that OPN protein, secretion and gene expression was elevated in the diabetic SUR1-E1506K+/+ islets...
November 24, 2017: Biochemical and Biophysical Research Communications
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