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https://www.readbyqxmd.com/read/28453726/osteopontin-rna-aptamer-can-prevent-and-reverse-pressure-overload-induced-heart-failure
#1
Jihe Li, Keyvan Yousefi, Wen Ding, Jayanti Singh, Lina A Shehadeh
Aims: Cardiac myocyte hypertrophy, the main compensatory response to chronic stress in the heart often progresses to a state of decompensation that can lead to heart failure. Osteopontin (OPN) is an effector for extracellular signalling that induces myocyte growth and fibrosis. Although increased OPN activity has been observed in stressed myocytes and fibroblasts, the detailed and long term effects of blocking OPN signalling on the heart remain poorly defined. Targeting cardiac OPN protein by an RNA aptamer may be beneficial for tuning down OPN pathologic signalling...
May 1, 2017: Cardiovascular Research
https://www.readbyqxmd.com/read/28446225/the-crosstalk-between-vascular-mscs-and-inflammatory-mediators-determines-the-pro-calcific-remodelling-of-human-atherosclerotic-aneurysm
#2
Carmen Ciavarella, Enrico Gallitto, Francesca Ricci, Marina Buzzi, Andrea Stella, Gianandrea Pasquinelli
BACKGROUND: Human mesenchymal stem cells (MSCs) possess well-known reparative abilities, but any defect of the immunomodulatory activity and/or the differentiation process may determine the development of human diseases, including those affecting the vascular wall. MSCs residing within the human aortic wall represent a potential cell mediator of atherosclerotic aneurysm development. METHODS: MSCs isolated from healthy and aneurysm aortas were characterized by flow cytometer and tested for differentiation properties...
April 26, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28445262/dose-dependence-of-pth-related-peptide-1-on-the-osteogenic-induction-of-mc3t3-e1-cells-in-vitro
#3
Jianping Wang, Jingfeng Li, Liang Yang, Yichi Zhou, Yi Wang
Parathyroid hormone (PTH), an 84-amino acid peptide, is an endocrine hormone that is secreted by parathyroid glands. PTH performs important functions in calcium regulation and bone remodeling. The PTH (1-34) named teriparatide, a 34-amino acid peptide derived from the N-terminus of PTH, conserves most of the functions of PTH, specifically the osteogenic capability. However, teriparatide is only used by injection and exhibits short duration. In addition, this PTH could not thoroughly expose active sites. In this study, a novel PTH-related peptide (designated PTHrP-1) derived from the N-terminus of PTH was added into the complete medium at different concentrations of PTHrP-1 (0, 50, 100, and 200 ng/mL) to induce the MC3T3-E1 cells...
April 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28441794/reciprocal-interactions-of-leukemic-cells-with-bone-marrow-stromal-cells-promote-enrichment-of-leukemic-stell-cell-compartments-in-response-to-curcumin-and-daunorubicin
#4
Saeed Mohammadi, Mohsen Nikbakht, Seyed Mehdi Sajjadi, Fariba Rad, Bahram Chahardouli, Javid Sabour Takanlu, Shahrbano Rostami, Kamran Alimoghaddam, Ardeshir Ghavamzadeh, Seyed H Ghaffari
A predominant challenge in developing curative leukemia therapy is interactions of leukemic cells with the bone marrow stromal microenvironment. We aimed to investigate the role of stromal cells, such as bone marrow mesenchymal stromal cells (BMSCs) and osteoblasts (OBs), in curcumin (CUR) and daunorubicin (DNR) induced apoptosis of acute myeloid leukemia (AML) cells. We used KG1 and U937 as leukemia cell line models and treated them with CUR and DNR. The cells were then co-cultured with BMSCs or a combination of BMSCs and OBs as feeders...
March 1, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28440483/human-osteopontin-potential-clinical-applications-in-cancer-review
#5
Chengcheng Hao, Yuxin Cui, Sionen Owen, Wenbin Li, Shan Cheng, Wen G Jiang
Human osteopontin (OPN) is a glycosylated phosphoprotein which is expressed in a variety of tissues in the body. In recent years, accumulating evidence has indicated that the aberrant expression of OPN is closely associated with tumourigensis, progression and most prominently with metastasis in several tumour types. In this review, we present the current knowledge on the expression profiles of OPN and its main splice variants in human cancers, as well as the potential implications in patient outcome. We also discuss its putative clinical application as a cancer biomarker and as a therapeutic target...
April 21, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28437177/periodontal-derived-mesenchymal-cell-sheets-promote-periodontal-regeneration-in-inflammatory-microenvironment
#6
Shujuan Guo, Jian Kang, Baohui Ji, Weihua Guo, Yi Ding, Yafei Wu, Weidong Tian
In this study, we investigated the periodontal regenerative potential of dental follicle cell (DFC) sheets and periodontal ligament cell (PDLC) sheets in the simulating inflammatory microenvironment of periodontitis, in order to confirm their regenerative potential for clinical application and explain the possible mechanism. The biological characteristics of DFC sheets and PDLC sheets were explored in lipopolysaccharid from Porphyromonas gingivalis (P. gingivalis LPS) induced inflammation microenvironment in vitro, then cell sheets were transplanted into canine periodontal defects with experimental periodontitis in situ for three months...
February 14, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28428079/mineralization-inhibiting-effects-of-transglutaminase-crosslinked-polymeric-osteopontin
#7
Betty Hoac, Valentin Nelea, Wenge Jiang, Mari T Kaartinen, Marc D McKee
Osteopontin (OPN) belongs to the SIBLING family (Small, Integrin-Binding LIgand N-linked Glycoproteins) of mineral-binding matrix proteins found in bones and teeth. OPN is a well-known inhibitor of matrix mineralization, and enzymatic modification of OPN can affect this inhibitory function. In bone, OPN exists both as a monomer and as a high-molecular-weight polymer - the latter is formed by transglutaminase-mediated crosslinking of glutamine and lysine residues in OPN to create homotypic protein assemblies...
April 18, 2017: Bone
https://www.readbyqxmd.com/read/28427342/intermittent-parathyroid-hormone-pth-promotes-cementogenesis-and-alleviates-the-catabolic-effects-of-mechanical-strain-in-cementoblasts
#8
Yuyu Li, Zhiai Hu, Chenchen Zhou, Yang Xu, Li Huang, Xin Wang, Shujuan Zou
BACKGROUND: External root resorption, commonly starting from cementum, is a severe side effect of orthodontic treatment. In this pathological process and repairing course followed, cementoblasts play a significant role. Previous studies implicated that parathyroid hormone (PTH) could act on committed osteoblast precursors to promote differentiation, and inhibit apoptosis. But little was known about the role of PTH in cementoblasts. The purpose of this study was to investigate the effects of intermittent PTH on cementoblasts and its influence after mechanical strain treatment...
April 20, 2017: BMC Cell Biology
https://www.readbyqxmd.com/read/28422962/docosahexaenoic-acid-blocks-progression-of-western-diet-induced-nonalcoholic-steatohepatitis-in-obese-ldlr-mice
#9
Kelli A Lytle, Carmen P Wong, Donald B Jump
BACKGROUND: Nonalcoholic fatty liver disease (NAFLD) is a major public health concern in western societies. Nonalcoholic steatohepatitis (NASH), the progressive form of NAFLD, is characterized by hepatic steatosis, inflammation, oxidative stress and fibrosis. NASH is a risk factor for cirrhosis and hepatocellular carcinoma. NASH is predicted to be the leading cause of liver transplants by 2020. Despite this growing public health concern, there remain no Food and Drug Administration (FDA) approved NASH treatments...
2017: PloS One
https://www.readbyqxmd.com/read/28420872/hairy-enhancer-of-split-homologue-1-suppresses-vascular-endothelial-growth-factor-induced-angiogenesis-via-downregulation-of-osteopontin-expression
#10
Xing-Xing Yao, Jing-Bo Lu, Zhi-Dong Ye, Lei Zheng, Qian Wang, Zhi-Qi Lin, Hao Liu, Heng Wan, Fang-Yong Fu, Xian-Ying Huang, Jian-Chen Xiu, Zheng-Jun Liu, Yan-Wei Hu
Angiogenesis plays a critical role in the progression and vulnerability of atherosclerotic plaques; however, the orchestration of angiogenesis in atherosclerotic plaque formation remains unclear. The results of microarray analysis, real-time PCR and immunohistochemical analyses showed that Hairy/enhancer of split homologue-1 (Hes-1) expression was significantly decreased, while that of osteopontin (OPN) was increased, in atherosclerotic plaques. Meanwhile, immunofluorescence results demonstrated that both Hes-1 and OPN were expressed in endothelial cells (ECs) of neovessels in atherosclerotic plaques...
April 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28419944/electrochemical-synthesis-of-three-dimensional-porous-reduced-graphene-oxide-film-preparation-and-in-vitro-osteogenic-activity-evaluation
#11
Zizhu Tian, Lixun Huang, Xibo Pei, Junyu Chen, Tong Wang, Tao Yang, Han Qin, Lei Sui, Jian Wang
In this study, three-dimensional reduced graphene oxide (3D-rGO) porous films were fabricated using a two-step electrochemical method, including an electrochemical deposition process for the self-assembly of GO and an electrochemical bubbling-based transfer. The morphology, physical properties, and phase composition of the 3D-rGO films were characterized, and the cellular bioactivities were evaluated using pre-osteoblasts (MC3T3-E1 cells). The attachment, proliferation and differentiation of the MC3T3-E1 cells on the 3D-rGO films was analyzed by scanning electron microscopy (SEM), Cell Counting Kit-8 (CCK-8) assays and live/dead cell staining, and alkaline phosphatase (ALP) activity assays, respectively...
April 12, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28418701/cementogenesis-is-inhibited-under-a-mechanical-static-compressive-force-via-piezo1
#12
Ying-Ying Zhang, Yi-Ping Huang, Hua-Xiang Zhao, Ting Zhang, Feng Chen, Yan Liu
OBJECTIVE: To investigate whether Piezo1, a mechanotransduction gene mediates the cementogenic activity of cementoblasts under a static mechanical compressive force. MATERIALS AND METHODS: Murine cementoblasts (OCCM-30) were exposed to a 2.0 g/cm(2) static compressive force for 3, 6, 12, and 24 hours. Then the expression profile of Piezo1 and the cementogenic activity markers osteoprotegerin (Opg), osteopontin (Opn), osteocalcin (Oc), and protein tyrosine phosphataselike member A (Ptpla) were analyzed...
April 18, 2017: Angle Orthodontist
https://www.readbyqxmd.com/read/28417915/high-glucose-accelerates-cell-proliferation-and-increases-the-secretion-and-mrna-expression-of-osteopontin-in-human-pancreatic-duct-epithelial-cells
#13
Miho Ito, Naohiko Makino, Akiko Matsuda, Yushi Ikeda, Yasuharu Kakizaki, Yoshihiko Saito, Yoshiyuki Ueno, Sumio Kawata
BACKGROUND: The incidence of pancreatic cancer is increasing year-by-year in Japan. Among the diseases that complicate pancreatic cancer, diabetes is the most common. Recently, it has become evident that patients suffering from diabetes and obesity show increased expression of osteopontin (OPN). The purpose of this study was to investigate the effect of high glucose and high insulin culture conditions on a human pancreatic duct epithelial cell line (HPDE-6), focusing particularly on OPN expression...
April 12, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28415624/hypoxia-inducible-factor-1a-induces-phenotype-switch-of-human-aortic-vascular-smooth-muscle-cell-through-pi3k-akt-aeg-1-signaling
#14
Kai Liu, Changcun Fang, Yuwen Shen, Zhengqin Liu, Min Zhang, Bingbing Ma, Xinyan Pang
To date, hypoxia-inducible factor 1a (HIF-1a) and astrocyte elevated gene-1 (AEG-1) have been involved in the proliferation, migration and morphological changes of vascular smooth muscle cells. However, the potential relationship of HIF-1a-AEG-1 pathway in human aortic smooth muscle cell (HASMC) has not been reported. In the present study, in-vitro assays were utilized to explore the potential impact of HIF-1a-AEG-1 signaling on HASMC phenotype. Here, we found that HIF-1a expression was up-regulated in the media of thoracic aortic dissection tissues as compared with normal aortic tissues, and was associated with increased apoptotic SMCs and decreased AEG-1 expression...
March 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28412087/pregestational-type-2-diabetes-mellitus-induces-cardiac-hypertrophy-in-the-murine-embryo-through-cardiac-remodeling-and-fibrosis
#15
Xue Lin, Penghua Yang, E Albert Reece, Peixin Yang
BACKGROUND: Cardiac hypertrophy is highly prevalent in patients with type 2 diabetes mellitus (T2DM). Experimental evidence has implied that pregnant women with T2DM and their children are at an increased risk of cardiovascular diseases. Our previous mouse model study has revealed that maternal T2DM induces structural heart defects in their offspring. OBJECTIVE: The present study aims to determine whether maternal T2DM induces embryonic heart hypertrophy in a murine model of diabetic embryopathy...
April 12, 2017: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28405192/induction-of-osteopontin-by-dengue-virus-3-infection-in-thp-1-cells-inhibition-of-the-synthesis-by-brefelamide-and-its-derivative
#16
Dyshelly N Pascapurnama, Hannah K M Labayo, Isolde Dapat, Divya D Nagarajegowda, Jingge Zhao, Jing Zhang, Osamu Yamada, Haruhisa Kikuchi, Shinichi Egawa, Yoshiteru Oshima, Haorile Chagan-Yasutan, Toshio Hattori
Osteopontin (OPN) is a multifunctional matricellular protein produced by a broad range of cells including osteoclasts, macrophages, T cells, endothelial cells, and vascular smooth muscle cells. OPN modulates various physiological and pathological events such as inflammation, wound healing, and bone formation and remodeling. Dengue virus (DENV) infection causes an increase in plasma OPN levels, which is correlated with the severity of symptoms and coagulation abnormalities. DENV infection also induces OPN gene expression in human macrophages...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28403169/osteopontin-activates-the-diabetes-associated-potassium-channel-talk-1-in-pancreatic-%C3%AE-cells
#17
Matthew T Dickerson, Nicholas C Vierra, Sarah C Milian, Prasanna K Dadi, David A Jacobson
Glucose-stimulated insulin secretion (GSIS) relies on β-cell Ca2+ influx, which is modulated by the two-pore-domain K+ (K2P) channel, TALK-1. A gain-of-function polymorphism in KCNK16, the gene encoding TALK-1, increases risk for developing type-2 diabetes. While TALK-1 serves an important role in modulating GSIS, the regulatory mechanism(s) that control β-cell TALK-1 channels are unknown. Therefore, we employed a membrane-specific yeast two-hybrid (MYTH) assay to identify TALK-1-interacting proteins in human islets, which will assist in determining signaling modalities that modulate TALK-1 function...
2017: PloS One
https://www.readbyqxmd.com/read/28397898/nmr-probing-and-visualization-of-correlated-structural-fluctuations-in-intrinsically-disordered-proteins
#18
Dennis Kurzbach, Andreas Beier, Agathe Vanas, Andrea G Flamm, Gerald Platzer, Thomas C Schwarz, Robert Konrat
A novel statistical analysis of paramagnetic relaxation enhancement (PRE) and paramagnetic relaxation interference (PRI) based nuclear magnetic resonance (NMR) data is proposed based on the computation of correlation matrices. The technique is demonstrated with an example of the intrinsically disordered proteins (IDPs) osteopontin (OPN) and brain acid soluble protein 1 (BASP1). The correlation analysis visualizes in detail the subtleties of conformational averaging in IDPs and highlights the presence of correlated structural fluctuations of individual sub-domains in IDPs...
April 19, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28395716/-insulin-like-growth-factor-1-promotes-the-proliferation-and-differentiation-of-human-periodontal-ligament-stem-cells-into-periodontal-ligament-fibroblasts
#19
Junxiu Qu, Lihui Jia, Caixin Che, Yuanyuan Yue, Jiangna Zhu, Chao Liu
Objective To investigate the effect of insulin-like growth factor 1 (IGF-1) on the proliferation and differentiation potential of human periodontal ligament stem cells (hPDLSCs). Methods The hPDLSCs were isolated by the limited dilution method. Surface markers of hPDLSCs were detected by flow cytometry. Cell clones were tested by crystal violet staining. The hPDLSCs were induced into osteoblasts and adipogenic by osteogenic and adipogenic medium. The influence of different concentrations of IGF-1 (1, 10, 30, 50, 100 ng/mL) on hPDLSCs was analyzed by CCK-8 assay...
April 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28394420/tiron-ameliorates-high-glucose-induced-cardiac-myocyte-apoptosis-by-pkc%C3%AE-dependent-inhibition-of-osteopontin
#20
Ping Jiang, Deling Zhang, Hong Qiu, Xianqi Yi, Yemin Zhang, Yingkang Cao, Bo Zhao, Zhongyuan Xia, Changhua Wang
Tiron functions as an effective antioxidant alleviating the intracellular reactive oxygen species (ROS) or the acute toxic metal overload. The previous studies have shown that cardiac myocyte apoptosis can be effectively inhibited by tiron administration in streptozotocin (STZ)-induced diabetic rats, primary neonatal rat cardiomyocytes (NRVMs), and H9c2 embryonic rat cardiomyocytes. However, the underlying signaling mechanism is ill-defined. In the present study, we found that tiron supplementation significantly inhibited apoptosis of high glucose (HG)-treated NRVMs and the left ventricular cardiomyocytes from STZ-diabetic rat, accompanied with a reduction of osteopontin (OPN) levels as well as an inhibition of PKCδ phosphorylation...
April 10, 2017: Clinical and Experimental Pharmacology & Physiology
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