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https://www.readbyqxmd.com/read/29678831/loss-of-p62-in-adipocytes-promotes-aggressive-prostate-cancer
#1
(no author information available yet)
OPN released from p62-deficient adipocytes enhances fatty-acid oxidation in tumor cells.
April 20, 2018: Cancer Discovery
https://www.readbyqxmd.com/read/29677209/osteopontin-plays-a-pivotal-role-in-increasing-severity-of-respiratory-syncytial-virus-infection
#2
Viviana Sampayo-Escobar, Ryan Green, Michael B Cheung, Raminder Bedi, Subhra Mohapatra, Shyam S Mohapatra
The molecular mechanisms underlying susceptibility to severe respiratory syncytial virus (RSV) infection remain poorly understood. Herein, we report on the role of osteopontin (OPN) in regulation of RSV infection in human epithelial cells and how interleukin-1 beta (IL-1β), a cytokine secreted soon after RSV infection, when persistently expressed can induce OPN expression leading to increased viral infection. We first compared OPN expression in two human epithelial cell lines: HEK-293 and HEp-2. In contrast to HEp-2, HEK-293 expresses low levels of pro-caspase-1 resulting in decreased IL-1β expression in response to RSV infection...
2018: PloS One
https://www.readbyqxmd.com/read/29677036/coupled-opg-fc-on-decellularized-aortic-valves-by-edc-nhs-attenuates-rat-mscs-calcification-in-vitro
#3
Qiao Zhang, Si Chen, Jiawei Shi, Fei Li, Xucong Shi, Xingjian Hu, Cheng Deng, Feng Shi, Guichun Han, Nianguo Dong
Valve calcification commonly damages natural human heart valves and tissue-engineered heart valves (TEHVs), and no ideal intervention is available in clinical practice. It is increasingly considered that osteoprotegerin (OPG) inhibits vascular calcification. We aimed to explore whether free OPG-Fc fusion protein or coupled OPG-Fc on decellularized aortic valves attenuates calcification. Calcification of rat bone marrow-derived mesenchymal stromal cells (MSCs) was induced by osteogenic differentiation media, and the effects of free OPG-Fc or OPG-Fc coupled on the decellularized porcine aortic heart valve leaflet scaffolds by coupling agents 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS) on calcification were observed...
April 18, 2018: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
https://www.readbyqxmd.com/read/29675422/elucidation-on-predominant-pathways-involved-in-the-differentiation-and-mineralization-of-odontoblast-like-cells-by-selective-blockade-of-mitogen-activated-protein-kinases
#4
Jia Tang, Takashi Saito
Aim: To analyze the effect of three mitogen-activated protein kinase (MAPK) inhibitors, namely, SB202190 (p38 inhibitor), SP600125 (JNK inhibitor), and PD98059 (ERK inhibitor) in Dex-stimulated MDPC-23 cell differentiation and mineralization. Methods: Experiment was divided into five groups, control (cells without Dex and inhibitors treatment), Dex (cells with Dex treatment but without inhibitors), Dex + SB202190, Dex + SP600125, and Dex + PD98059. Cell differentiation was assessed by alkaline phosphatase (ALP) activity assay and real time RT-PCR...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29672822/identification-of-novel-diagnostic-biomarkers-for-deep-venous-thrombosis
#5
Ashfaque A Memon, Kristina Sundquist, Mirnabi PirouziFard, Johan L Elf, Karin Strandberg, Peter J Svensson, Jan Sundquist, Bengt Zöller
The combination of a negative D-dimer and a Wells score can rule out, but not confirm, a diagnosis of deep venous thrombosis (DVT). We aimed to identify new diagnostic biomarkers for DVT and to investigate their relationship with hypercoagulability markers [D-dimer and activated protein C-protein C inhibitor (APC-PCI) complex]. We screened 92 cardiovascular-specific proteins in plasma samples from 45 confirmed DVT patients and 45 age- and sex-matched non-DVT patients selected from a prospective multicentre diagnostic management study (SCORE) by Proseek Multiplex CVDIII96×96 ...
April 19, 2018: British Journal of Haematology
https://www.readbyqxmd.com/read/29669827/osteopontin-regulates-hcv-replication-and-assembly-via-interaction-with-hcv-proteins-and-lipid-droplets-and-by-binding-to-receptors-%C3%AE-v%C3%AE-3-and-cd44
#6
Jawed Iqbal, Mehuli Sarkar-Dutta, Steven McRae, Akshaya Ramachandran, Binod Kumar, Gulam Waris
Hepatitis C virus (HCV) replication and assembly occur at the specialized site of endoplasmic reticulum (ER) membranes and lipid droplets (LDs), respectively. Recently, several host proteins have been shown to be involved in HCV replication and assembly. In the present study, we demonstrate the important relationship among osteopontin (OPN), ER and LDs. OPN is a secreted phosphoprotein and overexpression of OPN in hepatocellular carcinoma (HCC) tissue can lead to invasion and metastasis. OPN expression is also enhanced in HCV-associated HCC...
April 18, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29668569/osteopontin-levels-in-human-milk-vary-across-countries-and-within-lactation-period-data-from-a-multicenter-study
#7
Signe Bruun, Lotte Neergaard Jacobsen, Xiaolei Ze, Steffen Husby, Hiroshi M Ueno, Keisuke Nojiri, Shunjiro Kobayashi, Jungil Kwon, Xihong Liu, Shuyuan Yan, Jiyeon Yang, Gitte Zachariassen, Liang Chen, Wei Zhou, Brian Christensen, Esben S Sørensen
OBJECTIVES: Osteopontin (OPN) is a multifunctional protein expressed in many cell types, tissues and body fluids with the highest concentrations found in milk; significantly higher in human than in bovine milk. Intervention studies have indicated beneficial effects of supplementing infant formula with bovine OPN. In this multicenter study, we determined the OPN content in human milk samples from 629 Chinese, Danish, Japanese and Korean mothers. METHODS: At each study site, milk samples were collected and analyzed for OPN and protein concentration using ELISA and infrared spectroscopy, respectively...
April 17, 2018: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/29661034/the-use-of-chitosan-pla-nano-fibers-by-emulsion-eletrospinning-for-periodontal-tissue-engineering
#8
Renze Shen, Weihong Xu, Yanxiang Xue, Luyuan Chen, Haicheng Ye, Enyi Zhong, Zhanchao Ye, Jie Gao, Yurong Yan
OBJECTIVE: In this study, nanofibrous scaffolds base on pure polylactic acid (PLA) and chitosan/PLA blends were fabricated by emulsion eletrospinning. By modulating their mechanical and biological properties, cell-compatible and biodegradable scaffolds were developed for periodontal bone regeneration. METHODS: Pure PLA and different weight ratios of chitosan nano-particle/PLA nano-fibers were fabricated by emulsion eletrospinning. Scanning electron microscope (SEM) was performed to observe the morphology of nano-fibers...
April 16, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29650411/interfacial-mechanical-behaviour-of-protein-mineral-nanocomposites-a-molecular-dynamics-investigation
#9
Zheng Bo Lai, Ruixiang Bai, Zhenkun Lei, Cheng Yan
Biological composite materials, such as bone, tooth and nacre, are comprised of a mixture of nano-sized hard components (e.g. mineral platelets) and soft components (e.g. protein molecules). Their mechanical behaviour greatly depends on the protein-mineral interfaces. This paper investigates the effects of mineral surface nanostructures on the interfacial interaction and mechanical behaviour of protein-mineral nanocomposites. Interfacial shear between osteopontin (OPN) and hydroxyapatite (HA) mineral layers with surface nanostructures is investigated using the atomistic molecular dynamics (MD) simulations...
April 3, 2018: Journal of Biomechanics
https://www.readbyqxmd.com/read/29645344/functional-and-oncologic-outcomes-of-open-laparoscopic-and-robotic-partial-nephrectomy-a-multicenter-comparative-matched-pair-analyses-with-a-median-5-years-follow-up
#10
Ki Don Chang, Ali Abdel Raheem, Kwang Hyun Kim, Cheol Kyu Oh, Sung Yul Park, Young Sik Kim, Won Sik Ham, Woong Kyu Han, Young Deuk Choi, Byung Ha Chung, Koon Ho Rha
PURPOSE: Data regarding the long-term oncologic and functional outcomes of robotic-assisted, laparoscopic, and open partial nephrectomy (RPN, LPN and OPN) is lacking. Our aim was to compare outcomes among RPN, LPN and OPN with a 5-year median follow-up. PATIENTS AND METHODS: We retrospectively analyzed 1308 patients who underwent PN (RPN, n=380; LPN, n=206; OPN, n=722) between 2006 and 2012 at four academic centers. We performed 1:1:1 propensity score matching adjustment based on confounding variables among groups and 366 patients (122 in each group) were included in the final analysis...
April 12, 2018: BJU International
https://www.readbyqxmd.com/read/29627497/osteoblast-secreted-wisp-1-promotes-adherence-of-prostate-cancer-cells-to-bone-via-the-vcam-1-integrin-%C3%AE-4%C3%AE-1-system
#11
An-Chen Chang, Po-Chun Chen, Yu-Feng Lin, Chen-Ming Su, Ju-Fang Liu, Tien-Huang Lin, Show-Mei Chuang, Chih-Hsin Tang
Bone metastasis is a frequent occurrence in prostate cancer (PCa) that is associated with severe complications such as fracture, bone pain and hypercalcemia. The cross-talk between metastatic cancer cells and bone is critical to the development and progression of bone metastases. In our previous data, we have described how the involvement of the Wnt-induced secreted protein-1/vascular cell adhesion molecule-1 (WISP-1/VCAM-1) system in this tumor-bone interaction contributes to human PCa cell motility. In this study, we found that WISP-1 regulates bone mineralization by inducing bone morphogenetic protein-2 (BMP2), BMP4 and osteopontin (OPN) expression in osteoblasts...
April 5, 2018: Cancer Letters
https://www.readbyqxmd.com/read/29626154/biological-role-of-site-specific-o-glycosylation-in-cell-adhesion-activity-and-phosphorylation-of-osteopontin
#12
Midori Oyama, Yoshinobu Kariya, Yukiko Kariya, Kana Matsumoto, Mayumi Kanno, Yoshiki Yamaguchi, Yasuhiro Hashimoto
Osteopontin (OPN) is an extracellular glycosylated phosphoprotein that promotes cell adhesion by interacting with several integrin receptors. We previously reported that an OPN mutant lacking five O -glycosylation sites (Thr134 /Thr138 /Thr143 /Thr147 /Thr152 ) in the threonine/proline-rich region increased cell adhesion activity and phosphorylation compared to the wild type. However, the role of O -glycosylation in cell adhesion activity and phosphorylation of OPN remains to be clarified. Here, we show that site-specific O -glycosylation in the threonine/proline-rich region of OPN affects its cell adhesion activity and phosphorylation independently and/or synergistically...
April 6, 2018: Biochemical Journal
https://www.readbyqxmd.com/read/29626090/a-gtpase-activating-protein-binding-protein-g3bp1-antiviral-protein-relay-conveys-arteriosclerotic-wnt-signals-in-aortic-smooth-muscle-cells
#13
Bindu Ramachandran, John N Stabley, Su-Li Cheng, Abraham S Behrmann, Austin Gay, Li Li, Megan Mead, Julia Kozlitina, Andrew Lemoff, Hamid Mirzaei, Zhijian Chen, Dwight A Towler
In aortic vascular smooth muscle (VSM), the canonical Wnt receptor LRP6 inhibits protein arginine (Arg) methylation, a new component of noncanonical Wnt signaling that stimulates nuclear factor of activated T cells (viz., NFATc4). To better understand how methylation mediates these actions, mass spectrometry was performed on VSM cell extracts from control and LRP6-deficient mice. LRP6-dependent Arg methylation was regulated on > 500 proteins; only 21 exhibited increased monomethylation (MMA) with concomitant reductions in dimethylation...
April 6, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29624568/resveratrol-attenuates-lipopolysaccharides-lps-induced-inhibition-of-osteoblast-differentiation-in-mc3t3-e1-cells
#14
Jun Ma, Zhu Wang, Jianquan Zhao, WuJun Miao, TianWen Ye, Aimin Chen
BACKGROUND LPS-inhibited osteoblastic differentiation plays an important role in the pathogenesis of osteomyelitis. Thus, searching for drugs that affect LPS-mediated osteoblastic differentiation may be crucial in developing therapies for osteomyelitis. The purpose of this study was to investigate the role and mechanisms of resveratrol, a natural polyphenol present in red wine, on LPS-inhibited osteoblastic differentiation. MATERIAL AND METHODS Cell viability was measured by MMT assay. Mitochondrial ATP levels, membrane potential, and superoxide production were measured to evaluate the effects of LPS and resveratrol on mitochondrial functions in osteoblast-like MC3T3-E1 cells...
April 6, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29620234/crocin-prevents-platelet%C3%A2-derived-growth-factor-bb%C3%A2-induced-vascular-smooth-muscle-cells-proliferation-and-phenotypic-switch
#15
Lijian Tong, Guoxian Qi
The phenotypic switch of vascular smooth muscle cells (VSMCs) is a major initiating factor for atherosclerotic cardiovascular diseases. Platelet‑derived growth factor‑BB (PDGF‑BB) initiates a number of biological processes that contribute to VSMC proliferation and phenotypic switch. Crocin, a component of saffron, has been reported to inhibit atheromatous plaque formation. However, the effects of crocin on PDGF‑BB‑induced VSMC proliferation and phenotypic switch remain unclear. The aim of the present study was to investigate the role of crocin on PDGF‑BB‑induced VSMCs proliferation and phenotypic switch and its underlying mechanisms...
April 5, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29616117/correlation-of-hypoxia-status-with-radiosensitizing-effects-of-sodium-glycididazole-a-preclinical-study
#16
Yang Yu, Xiaolin Li, Hengwei Xu, Jing Liu, Min Dong, Jia Yang, Lu Sun, Xiaorong Sun, Ligang Xing
The correlation of pretreatment hypoxia status with the radiosensitization effect of sodium glycididazole (CMNa) was not previously defined. The purpose of the present study was to evaluate the tumor hypoxia status in various cancer xenografts and to investigate the correlation between tumor hypoxia status and radiosensitizing effects of CMNa based on the pharmacokinetic and pharmacodynamic parameters. Human esophageal cancer (EC109), head and neck cancer (FaDu) and lung cancer (A549) nude mice xenografts were used...
May 2018: Oncology Letters
https://www.readbyqxmd.com/read/29607144/serum-osteopontin-in-patients-with-lung-cancer-and-chronic-obstructive-pulmonary-disease-does-the-co-existence-make-the-difference
#17
Vasiliki Petta, Stelios Loukides, Konstantinos Kostikas, Andriana I Papaioannou, Georgios Papatheodorou, Kyriaki Cholidou, Ioannis Tomos, Spyridon Papiris, Nikolaos G Koulouris, Petros Bakakos
Background: Osteopontin (OPN) is involved in cancer development and metastasis. Increased sputum OPN was detected in chronic obstructive pulmonary disease (COPD). Methods: We evaluated serum OPN levels in patients with lung cancer (LC) and/or COPD and aimed to determine OPN prognostic performance in 1-year mortality in LC and also its diagnostic performance in LC among COPD patients. We recruited 167 LC patients, 85 with concomitant COPD. 28 COPD patients served as control group...
February 2018: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/29602294/melatonin-ameliorates-brain-oxidative-stress-and-upregulates-senescence-marker-protein-30-and-osteopontin-in-a-rat-model-of-vascular-dementia
#18
I Bin-Jaliah, H F Sakr
The aim of this study was to investigate the effect of melatonin on oxidative stress and senescence marker protein-30 (SMP30) as well as osteopontin (OPN) expression in the hippocampus of rats subjected to vascular dementia (VD). A total of 72 male rats were divided into six groups (n = 12 each) as follows: (i) untreated control (CON), (ii) sham-operated group, (iii) sham-operated + melatonin, (iv) rats exposed to VD induced by permanent bilateral occlusion of the common carotid arteries (BCCAO) leading to chronic cerebral hypoperfusion, (v) rats exposed to VD + melatonin, and (vi) rats exposed to VD + donepezil (DON)...
March 1, 2018: Physiology International
https://www.readbyqxmd.com/read/29601800/overexpression-of-mir-182-inhibits-ossification-of-ligamentum-flavum-cells-by-targeting-nampt
#19
Qiang Zhang, Yifei Shen, Yuqing Jiang, Shujie Zhao, Dong Zhou, Nanwei Xu
Ossification of the ligamentum flavum (OLF) is a debilitating disease resulting from the development of ectopic bone formation, which leads to the compression of the spinal cord. Nicotinamide phosphoribosyltransferase (NAMPT) was found to be upregulated and microRNA-182 (miR-182) was downregulated in OLF tissue. We investigated the effects of NAMPT and miR-182 expression in OLF cells and the influence on proteins associated with osteogenic differentiation. MiR-182 overexpression inhibited NAMPT, RUNX2, OCN and OPN mRNA and protein expression in OLF cells...
March 27, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29600966/asymmetric-dimethylarginine-as-a-useful-risk-marker-of-radial-artery-calcification-in-patients-with-advanced-kidney-disease
#20
Marcin Krzanowski, Katarzyna Krzanowska, Mariusz Gajda, Paulina Dumnicka, Grzegorz Kopeć, Bartłomiej Guzik, Karolina Woziwodzka, Artur Dziewierz, Jan A Litwin, Władysław Sułowicz
INTRODUCTION    Medial arterial calcification is common in patients with chronic kidney disease (CKD) and is considered a risk factor for morbidity and mortality. OBJECTIVES    We aimed to evaluate the correlation between asymmetric dimethylarginine (ADMA) levels, radial artery calcification, and common carotid artery intima-media thickness (CCA‑IMT). PATIENTS AND METHODS    The study included 51 patients with CKD, in whom an arteriovenous fistula for hemodialysis access was created to collect radial artery samples for a histological examination, and 33 healthy volunteers, in whom the reference concentrations of ADMA were assessed...
March 29, 2018: Polish Archives of Internal Medicine
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