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https://www.readbyqxmd.com/read/28645880/biomarkers-of-airway-and-systemic-inflammation-in-obese-asthmatic-paediatric-patients
#1
H T Nacaroglu, O B Gayret, M Erol, O Buke, O Zengi, M Tasdemir, Z Tasdemir, O Yigit
BACKGROUND: It is thought that airway inflammation is more common in obese asthmatic patients because inflammation is harder to control and does not respond well to glucocorticoid treatment. OBJECTIVE: This study's aim was to investigate the effect of obesity on airway and systemic inflammation in children with asthma and to identify the biomarkers that play a role in this inflammation. METHODS: The study included patients aged 6-16 years who were diagnosed with asthma in the paediatric allergy outpatient clinic of Bagcilar Training and Research Hospital in Turkey...
June 20, 2017: Allergologia et Immunopathologia
https://www.readbyqxmd.com/read/28644145/colorectal-carcinoma-with-osseous-metaplasia
#2
Xibo Liu, Jinghong Xu, Lirong Chen
Osseous metaplasia (OM) is rarely observed in colorectal cancer (incidence < 0.4% in rectal cancer), where it has a non-specific clinical presentation and unknown pathogenesis. Here, we report three cases of colorectal carcinoma with OM and propose a new hypothesis. All three patients (two males and one female) were Chinese and had different sites of colorectal carcinoma with OM: rectum, sigmoid colon, and appendix. The pathologic diagnoses were serrated adenocarcinoma; moderately to poorly differentiated adenocarcinoma with micropapillary carcinoma and cribriform comedo-type adenocarcinoma; and mucinous adenocarcinoma, respectively...
June 20, 2017: Oncotarget
https://www.readbyqxmd.com/read/28643442/the-effect-of-collagen-coating-on-titanium-with-nanotopography-on-in-vitro-osteogenesis
#3
Daniel G Costa, Emanuela P Ferraz, Rodrigo P F Abuna, Paulo T de Oliveira, Marco Morra, Marcio M Beloti, Adalberto L Rosa
Several studies have shown the positive effects of Ti either with nanotopography or coated with collagen on osteoblast differentiation. Thus, we hypothesized that the association of nanotopography with collagen may increase the in vitro osteogenesis on Ti surface. Ti discs with nanotopography with or without collagen coating were characterized by scanning electron microscopy and atomic force microscopy. Rat calvaria-derived osteoblastic cells were cultured on both Ti surfaces for up to 14 days and the following parameters were evaluated: cell proliferation, alkaline phosphatase (ALP) activity, extracellular matrix mineralization, protein expression of bone sialoprotein (BSP) and osteopontin (OPN), and gene expression of collagen type 1a (Coll1a), runt related transcription factor 2 (Runx2), osterix (OSX), osteocalcin (OC), Ki67, Survivin and Bcl2 associated X protein (BAX)...
June 23, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28639943/bioprintable-form-of-chitosan-hydrogel-for-bone-tissue-engineering
#4
T Tolga Demirtas, Gülseren Irmak, Menemse Gumusderelioglu
Bioprinting can be defined as the 3D patterning of living cells and other biologics by using a computer-aided layer-by-layer deposition approach. Presence of cells within the ink to usage of a "bio-ink" presents the potential to print of living tissues and organ analogs that can be implanted into damaged/diseased tissue to promote highly controlled cell-based regeneration. In this study, it was shown for the first time that chitosan solution and its composite with nanostructured bone-like hydroxyapatite (HA) can be mixed with cells and printed successfully...
June 22, 2017: Biofabrication
https://www.readbyqxmd.com/read/28638999/osteopontin-and-clusterin-levels-in-type-2-diabetes-mellitus-differential-association-with-peripheral-autonomic-nerve-function
#5
Raelene E Maser, M James Lenhard, Ryan T Pohlig, P Babu Balagopal
Osteopontin (OPN) and clusterin are secreted glycoproteins potentially associated with nerve function. Sudomotor dysfunction is associated with the development of foot ulcerations. The purpose of this study was to investigate the potential relationship of OPN and clusterin with sudomotor function (i.e., autonomic nerves that control sweating) in participants with type 2 diabetes mellitus (T2DM). Sudomotor function was assessed using SUDOSCAN® which measures electrochemical skin conductance (ESC) of the hands and feet...
June 21, 2017: Neurological Sciences
https://www.readbyqxmd.com/read/28638450/osteopontin-is-critical-for-hyperactive-mtor-induced-tumorigenesis-in-oral-squamous-cell-carcinoma
#6
Ning Gan, Sihai Zou, Wenming Hang, Deqin Yang, Xuemei Zhang, Yibing Yin
Mechanistic target of rapamycin (mTOR) plays a critical role in the development of oral squamous cell carcinoma (OSCC), but the underlying mechanisms remain poorly understood. Here we have demonstrated that the expression of osteopontin (OPN) was dramatically up-regulated in OSCC tissues and cell lines. Moreover, reduction of OPN suppressed cell proliferation, colony formation, and in vivo tumorigenic ability of OSCC cell lines Tca8113. In addition, there was a strong positive correlation between mTORC1 activity and OPN expression in OSCC tissues and cell lines...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/28637282/the-effect-of-predisposing-atheroembolic-risk-factors-on-renal-functional-recovery-between-laparoscopy-and-open-technique-in-patients-with-t1-stage-renal-cell-carcinoma-who-underwent-partial-nephrectomy-a-retrospective-comparison-study
#7
Sung Han Kim, Jae Young Joung, Ho Kyung Seo, Kang Hyun Lee, Jinsoo Chung
Objective: The present study aimed to determine the effect of an increasing number of predisposing atheroembolic risk factors on the development of chronic kidney disease (CKD) after partial nephrectomy (PN) in patients with T1-stage renal cell carcinoma (RCC). Methods: The study included 147 patients with T1-stage RCC with a normal contralateral kidney and without preoperative CKD, who underwent open (OPN, N = 83, 56.5%) or laparoscopic PN (LPN, N = 64, 43.5%) between 2003 and 2014...
June 14, 2017: Japanese Journal of Clinical Oncology
https://www.readbyqxmd.com/read/28634845/histomorphometric-and-immunohistochemical-evaluation-of-collagen-containing-xenogeneic-bone-blocks-used-for-lateral-bone-augmentation-in-staged-implant-placement
#8
Alberto Ortiz-Vigón, Sergio Martinez-Villa, Iñaki Suarez, Fabio Vignoletti, Mariano Sanz
BACKGROUND: The osteoconductive properties of collagen containing xenogeneic bone blocks (CCXBB) remain unclear. The aim of this prospective single-arm clinical study was to assess the histological outcomes of CCXBB blocks used as bone replacement grafts for lateral bone augmentation procedures. METHODS: In 15 patients with severe horizontal alveolar ridge resorption, lateral augmentation procedures were performed using CCXBB as bone replacement grafts. Twenty-six weeks postoperatively, a re-entry procedure was performed to evaluate the bone width for adequate implant placement and two histological specimens were retrieved from each patient, one being processed for ground sectioning and the other for decalcified paraffin-included sections...
December 2017: International Journal of Implant Dentistry
https://www.readbyqxmd.com/read/28627641/osteopontin-deficiency-protects-mice-from-cholesterol-gallstone-formation-by-reducing-expression-of-intestinal-npc1l1
#9
Jing Lin, Wei-Qing Shao, Qing-Zhi Chen, Wen-Wei Zhu, Lu Lu, Hu-Liang Jia, Jin-Hong Chen
Homeostasis of cholesterol is regulated by absorption in the intestine and synthesis in the liver. The authors previously demonstrated that OPN (osteopontin) exhibits the ability to alter hepatic cholesterol metabolism, thus affecting cholesterol gallstone formation in mice. The present study investigated the role of OPN in cholesterol gallstone formation, focusing on its effect on intestinal absorption of cholesterol. OPN gene knockout (OPN‑/‑) mice and wild‑type mice were respectively fed with a chow or lithogenic diet (LD) for 8 weeks...
June 14, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28625769/mechanisms-of-aortic-dissection-smooth-muscle-cell-phenotype-switch
#10
Zhao An, Yang Liu, Zhi-Gang Song, Hao Tang, Yang Yuan, Zhi-Yun Xu
OBJECTIVE: To investigate the expression of Nanog homeobox (NANOG) in thoracic aortic dissection (TAD) and the role of NANOG in regulating human aortic vascular smooth muscle cells (VSMCs) phenotype switch. METHODS: Aortic specimens were collected from 20 patients undergoing TAD and 10 controls. VSMCs were isolated by adherent cultivation approach. The expression of NANOG, osteopontin (OPN), and VSMCs phenotype markers were determined by quantitative real-time polymerase chain reaction, Western blot, immunohistochemistry, and immunofluorescence...
May 25, 2017: Journal of Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/28621452/osteogenic-commitment-and-differentiation-of-human-mesenchymal-stem-cells-by-low-intensity-pulsed-ultrasound-stimulation
#11
Viviana Costa, Valeria Carina, Simona Fontana, Angela De Luca, Francesca Monteleone, Stefania Pagani, Maria Sartori, Stefania Setti, Cesare Faldini, Riccardo Alessandro, Milena Fini, Gianluca Giavaresi
Low-intensity pulsed ultrasound (LIPUS) as an adjuvant therapy in in vitro and in vivo bone engineering has proven to be extremely useful. The present study aimed at investigating the effect of 30 mW/cm(2) LIPUS stimulation on commercially available human mesenchymal stem cells (hMSCs) cultured in basal or osteogenic medium at different experimental time points (7d, 14d, 21d). The hypothesis was that LIPUS would improve the osteogenic differentiation of hMSC and guarantying the maintenance of osteogenic committed fraction, as demonstrated by cell vitality and proteomic analysis...
June 16, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28620344/the-role-of-osteopontin-and-its-gene-on-glucocorticoid-response-in-myasthenia-gravis
#12
Yanchen Xie, Hai-Feng Li, Liang Sun, Linda L Kusner, Shuhui Wang, Yunxiao Meng, Xu Zhang, Yu Hong, Xiang Gao, Yao Li, Henry J Kaminski
Biomarkers that assess treatment response for patients with the autoimmune disorder, myasthenia gravis (MG), have not been evaluated to a significant extent. We hypothesized the pro-inflammatory cytokine, osteopontin (OPN), may be associated with variability of response to glucocorticoids (GCs) in patients with MG. A cohort of 250 MG patients treated with standardized protocol of GCs was recruited, and plasma OPN and polymorphisms of its gene, secreted phosphoprotein 1 (SPP1), were evaluated. Mean OPN levels were higher in patients compared to healthy controls...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28608628/the-role-of-microrna-143-5p-in-the-differentiation-of-dental-pulp-stem-cells-into-odontoblasts-by-targeting-runx2-via-the-opg-rankl-signaling-pathway
#13
Fu-Liang Zhan, Xin-Yang Liu, Xing-Bo Wang
This study aims to elucidate the mechanisms by which microRNA-143-5p (miR-143-5p) targets runt-related transcription factor 2 (Runx2) in the differentiation of dental pulp stem cells (DPSCs) into odontoblasts, through regulating the osteoprotegerin receptor activator of the nuclear factor-κB ligand (OPG/RANKL) signaling pathway. Following transfection, DPSCs were divided into blank, control, miR-143-5p mimics, miR-143-5p inhibitors, miR-143-5p inhibitors + siRunx2 and siRunx2 groups. Alkaline phosphatase (ALP) activity and mineralized nodules were detected using ALP kit and alizarin red staining...
June 13, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28608614/solanum-muricatum-promotes-osteogenic-differentiation-of-rat-bone-marrow-stromal-cells
#14
Nan Wang, Feng Wang, Youshui Gao, Zubin Zhou, Wei Liu, Chenhao Pan, Peipei Yin, Mingjie Tang, Xiaowei Yu
Solanum muricatum (SM), also known as pepino, is known for its antioxidative and anti-inflammatory effects. The aim of this study was to evaluate the effects of SM extract in promoting osteogenic differentiation and regulating the Wnt and bone morphogenetic protein (BMP) signaling pathways. Ingredients of pepino were extracted and identified. SM extracts were used to treat rat bone marrow stromal cells (BMSCs), followed by evaluating alkaline phosphatase activities and mineralization levels. The mRNA levels of osteogenic biomarkers, including OPN and Collagen I, were also evaluated with real-time polymerase chain reaction...
June 13, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28604249/sustained-release-of-melatonin-from-tio2-nanotubes-for-modulating-osteogenic-differentiation-of-mesenchymal-stem-cells-in-vitro
#15
Min Lai, Ziyang Jin, Qiang Tang, Min Lu
To control the sustained release of melatonin and modulate the osteogenic differentiation of mesenchymal stem cells (MSCs), melatonin was firstly loaded onto TiO2 nanotubes by direct dropping method, and then a multilayered film was coated by a spin-assisted layer-by-layer technique, which was composed of chitosan (Chi) and gelatin (Gel). Successful fabrication was characterized by field emission scanning electron microscopy, atomic force microscope, X-ray photoelectron spectroscopy and contact angle measurement, respectively...
June 19, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28601359/osteopontin-exacerbates-pseudomonas-aeruginosa-induced-bacteremia-in-mice
#16
Zhenghao Piao, Haiying Yuan
Osteopontin (OPN) is a multifunctional protein involved in various pathophysiological processes. However, the role of OPN in Pseudomonas aeruginosa-related sepsis is not yet clear. Here, we found that OPN expression was elevated in plasma and spleen samples from P. aeruginosa-infected mice. To determine the function of OPN in sepsis, we used wild-type (WT) and OPN-knockout (KO) mice with P. aeruginosa-induced bacteremia. We found that OPN-KO mice exhibited reduced mortality compared with WT mice and that OPN exacerbated spleen bleeding and functional impairment...
May 30, 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28595626/osteopontin-enhances-multi-walled-carbon-nanotube-triggered-lung-fibrosis-by-promoting-tgf-%C3%AE-1-activation-and-myofibroblast-differentiation
#17
Jie Dong, Qiang Ma
BACKGROUND: Carbon nanotubes (CNTs) have been used in a variety of applications because of their unique properties and functions. However, many CNTs have been shown to induce lung fibrosis in experimental animals with some at a potency greater than that of silica, raising concern over possible toxic effects of CNT exposure in humans. Research into the mechanisms by which CNTs induce pulmonary fibrosis is warranted in order to facilitate the understanding, monitoring, and treatment of CNT-induced lung lesions that might occur in exposed populations...
June 8, 2017: Particle and Fibre Toxicology
https://www.readbyqxmd.com/read/28595270/spp1-genotype-and-glucocorticoid-treatment-modify-osteopontin-expression-in-duchenne-muscular-dystrophy-cells
#18
Sara Vianello, Boris Pantic, Aurora Fusto, Luca Bello, Eva Galletta, Doriana Borgia, Bruno F Gavassini, Claudio Semplicini, Gianni Sorarù, Libero Vitiello, Elena Pegoraro
Glucocorticoids are beneficial in Duchenne muscular dystrophy (DMD). Osteopontin (OPN), the protein product of SPP1, plays a role in DMD pathology modulating muscle inflammation and regeneration. A polymorphism in the SPP1 promoter (rs28357094) has been recognized as a genetic modifier of DMD, and there is evidence suggesting that it modifies response to glucocorticoid treatment. The effect of the glucocorticoid deflazacort on SPP1 mRNA and protein expression was investigated in DMD primary human myoblasts and differentiated myotubes with defined rs28357094 genotype (TT versus TG)...
June 8, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/28590075/malat1-mir-127-5p-regulates-osteopontin-opn-mediated-proliferation-of-human-chondrocytes-through-pi3k-akt-pathway
#19
Jun Liang, Li Xu, Feng Zhou, An-Min Liu, Hong-Xing Ge, Yuan-Yuan Chen, Min Tu
Osteoarthritis (OA) is characterized by progressive destruction of articular cartilage, resulting in significant disability. Chondrocytes present in various types of cartilage and are responsible for the growth and maintenance of the tissue. Over-proliferation of human chondrocytes may contributes to OA pathological process. Previously, we revealed that miR-127-5p could inhibit the proliferation of human chondrocytes through osteopontin (OPN). In the present study, we used online tools to figure out several candidates lncRNAs which were potentially correlated with miR-127-5p...
June 7, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28585833/energetic-basis-for-inhibition-of-calcium-phosphate-biomineralization-by-osteopontin
#20
Meng Li, Lijun Wang, Christine V Putnis
Calcium oxalate kidney stones form attached to Randall's plaques (RP), calcium phosphate (Ca-P) deposits on the renal papillary surface. Osteopontin (OPN) suppresses crystal growth in the complex process of urinary stone formation, but the inhibitory role of active domains of OPN involved in the initial formation of the RPs attached to epithelial cells has yet to be clarified. Here we demonstrate the thermodynamic basis for how OPN sequences regulate the onset of Ca-P mineral formation on lipid rafts as a model membrane...
June 12, 2017: Journal of Physical Chemistry. B
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