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https://www.readbyqxmd.com/read/29024250/non-invasive-detection-of-bladder-cancer-via-expression-targeted-gene-delivery
#1
Yunlan Fang, B Wolfson, W T Godbey
BACKGROUND: Because of the time and expense associated with the procedures and possible distress to the patient, cystoscopy or other imaging techniques are typically not used for bladder cancer detection before symptoms become present. Alternatively, commercial assays for urinary tumor markers exist but are marred by low sensitivity and high cost. There is a need for a simple and sensitive means of tumor detection, such as via the analysis of urine. METHODS: Plasmids encoding the secretable reporter Gaussia luciferase (G...
October 11, 2017: Journal of Gene Medicine
https://www.readbyqxmd.com/read/29024060/loss-of-dnmt3b-in-chondrocytes-leads-to-delayed-endochondral-ossification-and-fracture-repair
#2
Cuicui Wang, Yousef Abu-Amer, Regis J O'Keefe, Jie Shen
Despite advanced understanding of signaling mediated by local and systemic factors, the role of epigenetic factors in the regulation of bone regeneration remains vague. The DNA methyltransferases (Dnmts) Dnmt3a and Dnmt3b have tissue specific expression patterns and create unique methylation signatures to regulate gene expression. Using a stabilized murine tibia fracture model we find that Dnmt3b is induced early in fracture healing, peaks at 10 days post fracture (dpf), and declines to nearly undetectable levels by 28 dpf...
October 10, 2017: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/29016864/loss-of-host-derived-osteopontin-creates-a-glioblastoma-promoting-microenvironment
#3
Frank Szulzewsky, Nina Schwendinger, Dilansu Güneykaya, Patrick J Cimino, Dolores Hambardzumyan, Michael Synowitz, Eric C Holland, Helmut Kettenmann
Background: Microglia and periphery-derived monocytes infiltrate human and mouse glioblastoma and their density is positively correlated with malignancy. Using microarray and RNA sequencing we have previously shown that glioblastoma-associated microglia/monocytes (GAMs) express osteopontin/SPP1. Methods: We used qRT-PCR, immunofluorescence stainings, western-blot, and flow-cytometry to identify the various sources of osteopontin (OPN) expression in human and mouse glioblastoma...
August 26, 2017: Neuro-oncology
https://www.readbyqxmd.com/read/28994761/optical-coherence-tomography-imaging-mouse-retinal-ganglion-cells-in-vivo
#4
Jolanta Jagodzinska, Emmanuelle Sarzi, Mélanie Cavalier, Marie Seveno, Volker Baecker, Christian Hamel, Marie Péquignot, Cecile Delettre
Structural changes in the retina are common manifestations of ophthalmic diseases. Optical coherence tomography (OCT) enables their identification in vivo-rapidly, repetitively, and at a high resolution. This protocol describes OCT imaging in the mouse retina as a powerful tool to study optic neuropathies (OPN). The OCT system is an interferometry-based, non-invasive alternative to common post mortem histological assays. It provides a fast and accurate assessment of retinal thickness, allowing the possibility to track changes, such as retinal thinning or thickening...
September 22, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28990259/isolation-and-screening-of-rare-actinobacteria-a-new-insight-for-finding-natural-products-with-anti-vascular-calcification-activity
#5
Fatemeh Salimi, Javad Hamedi, Elaheh Motevaseli, Fatemeh Mohammadipanah
AIM: Vascular calcification (VC) is a significant pathological process in some life-threatening diseases. Several pathological mechanisms, including transdifferentiation of vascular smooth muscle cells to osteoblast-like cells and apoptosis are involved in VC. Compounds with an inhibitory effect on these processes are potentially efficient medications. In consideration of the multiple biological activities of Actinobacteria, this research was aimed at finding anti-VC metabolite-producing Actinobacteria...
October 9, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28989133/gingival-crevicular-fluid-turnover-markers-in-premenopausal-vs-postmenopausal-women-receiving-orthodontic-treatment
#6
Anusha Bitra, B Jhansi Rani, Sanket S Agarkar, Anuj S Parihar, Gopinath P Vynath, Shekhar Grover
BACKGROUND: Orthodontic treatment is one of the commonly used dental treatments. Orthodontic forces act on the bone by modulating the biomolecules, chiefly the osteoprotegerin (OPG), osteopontin (OPN), receptor activator of nuclear factor kappa-B (RANK), and RANK ligand (RANKL) (OPG ligand). Hormonal changes are known to cause marked alteration in the levels of these biomolecules. Hence, we planned this study to evaluate the response of bone biomarkers in the gingival crevicular fluid (GCF) in postmenopausal women undergoing fixed orthodontic therapy...
October 1, 2017: Journal of Contemporary Dental Practice
https://www.readbyqxmd.com/read/28987763/endogenous-osteopontin-induces-myocardial-ccl5-and-mmp-2-activation-that-contributes-to-inflammation-and-cardiac-remodeling-in-a-mouse-model-of-chronic-chagas-heart-disease
#7
Eugenia Pérez Caballero, Miguel H Santamaría, Ricardo S Corral
Cardiac dysfunction with progressive inflammation and fibrosis is a hallmark of Chagas disease caused by persistent Trypanosoma cruzi infection. Osteopontin (OPN) is a pro-inflammatory cytokine that orchestrates mechanisms controlling cell recruitment and cardiac architecture. Our main goal was to study the role of endogenous OPN as a modulator of myocardial CCL5 chemokine and MMP-2 metalloproteinase, and its pathological impact in a murine model of Chagas heart disease. Wild-type (WT) and OPN-deficient (spp1 -/-) mice were parasite-infected (Brazil strain) for 100days...
October 4, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28986711/blockade-of-lgr4-inhibits-proliferation-and-odonto-osteogenic-differentiation-of-stem-cells-from-apical-papillae
#8
Meng Zhou, Shuyu Guo, Lichan Yuan, Yuxin Zhang, Mengnan Zhang, Huimin Chen, Mengting Lu, Jianrong Yang, Junqing Ma
During tooth root development, stem cells from apical papillae (SCAPs) are indispensable, and their abilities of proliferation, migration and odontoblast differentiation are linked to root formation. Leucine-rich repeat-containing GPCR 4 (LGR4) modulates the biological processes of proliferation and differentiation in multiple stem cells. In this study, we showed that LGR4 is expressed in all odontoblast cell lineage cells and Hertwig's epithelial root sheath (HERS) during the mouse root formation in vivo. In vitro we determined that LGR4 is involved in the Wnt/β-catenin signaling pathway regulating proliferation and odonto/osteogenic differentiation of SCAPs...
October 6, 2017: Journal of Molecular Histology
https://www.readbyqxmd.com/read/28986261/mmp-9-cleaved-osteopontin-isoform-mediates-tumor-immune-escape-by-inducing-expansion-of-myeloid-derived-suppressor-cells
#9
Lijuan Shao, Bo Zhang, Lingxiong Wang, Liangliang Wu, Quancheng Kan, Kexing Fan
As an extracellular matrix protein, osteopontin (OPN) has been shown to play an important role in regulation of the immune response to tumors. Myeloid-derived suppressor cells (MDSCs), a heterogeneous population of myeloid progenitors, are major components of the immune suppressive tumor microenvironment and contribute to tumor evasion of the immune response. However, the specific regulating mechanisms underlying MDSCs expansion remain unclear. Here, we found that MDSCs accumulated in the spleen and tumors of 3LL tumor-bearing mice...
October 4, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28978514/relationship-of-cx43-regulation-of-vascular-permeability-to-osteopontin-tight-junction-protein-pathway-after-sepsis-in-rats
#10
Jie Zhang, Guangming Yang, Yu Zhu, Xiaoyong Peng, Tao Li, Liangming Liu
Our previous study demonstrated that connexin (Cx)43 participates in the regulation of vascular permeability in severe sepsis. Whether OPN is involved in Cx43 regulation of vascular permeability after sepsis and the relationship with tight-junction proteins are not clear. In this study, we investigated the role of zo-1 and clauidn-5 in Cx43 regulation of vascular permeability and its relationship to OPN. The expression of zo-1 and claudin-5 were decreased in CLP rats and LPS-treated pulmonary vein VECs. Cx43 over-expressed lentivirus induced the degradation of zo-1 and claudin-5, while Cx43 RNAi lentivirus abrogate the degradation of zo-1 and cludin-5 induced by LPS...
October 4, 2017: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
https://www.readbyqxmd.com/read/28977834/decreased-wwox-expression-promotes-angiogenesis-in-osteosarcoma
#11
Jia Wen, Zongchao Xu, Jiazhen Li, Yingqiang Zhang, Wenzhe Fan, Yu Wang, Mingjian Lu, Jiaping Li
WWOX (WW domain-containing oxidoreductase) is known to be an important tumor suppressor in cancer. In this study, we used samples from 201 osteosarcoma patients to investigate the effects of WWOX on angiogenesis and invasion. WWOX levels were negatively correlated with RUNX2 and VEGF levels, but were not correlated with OPN levels. Among the clinicopathological characteristics examined, WWOX was associated only with response to neoadjuvant chemotherapy, and its expression in osteosarcoma tissues was a predictor of disease-free survival...
September 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28957406/osteopontin-activates-mesenchymal-stem-cells-to-repair-skin-wound
#12
Wenping Wang, Pei Li, Wei Li, Junzi Jiang, Yanyan Cui, Shirong Li, Zhenxiang Wang
Mesenchymal stem cells (MSCs) are promising candidates for skin wound repair due to their capabilities of accumulating at wounds and differentiating into multiple types of skin cells. However, the underlying mechanisms responsible for these processes remain unclear. In this study, we found that osteopontin (OPN) stimulated the migration of MSCs in vitro, and observed the recruitment of endogenous MSCs to a skin wound and their differentiation into keratinocytes and endothelial cells. In OPN knock-out mice, the recruitment of MSCs to the skin wound was significantly inhibited, and wound closure was hampered after an intradermal injection of exogenous MSCs compared to wild-type mice...
2017: PloS One
https://www.readbyqxmd.com/read/28952709/opn-b-and-c-isoforms-doubtless-veto-anti-angiogenesis-effects-of-curcumin-in-combination-with-conventional-aml-regiment
#13
Akram Mirzaei, Seyed H Ghaffari, Mohsen Nikbakht, Hosein Kamranzadeh Foumani, Mohammad Vaezi, Saeed Mohammadi, Kamran Alimoghaddam, Ardeshir Ghavamzadeh
Osteopontin (OPN) is an extracellular structural protein that is secreted by osteoblasts and hematopoietic cells. It suppresses the proliferation of hematopoietic stem and also plays an important role in promoting survival and drug resistance in leukemic stem cells (LSCs). Since the role of OPN isoforms in AML angiogenesis are remaining controversial, in the present study, we aimed to evaluate whether curcumin (CUR), as a known natural component with anti-angiogenesis effects, in a combination of AML conventional regiment has the potency to preclude induced anti-angiogenesis effects of OPN isoforms or not? Leukemia cells were treated with different concentration of CUR and AML conventional drugs alone and/or in combination with together to find effective doses and IC50 values...
September 27, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28950897/blind-bedside-postpyloric-placement-of-spiral-tube-as-rescue-therapy-in-critically-ill-patients-a-prospective-tricentric-observational-study
#14
Bo Lv, Linhui Hu, Lifang Chen, Bei Hu, Yanlin Zhang, Heng Ye, Cheng Sun, Xiunong Zhang, Huilan Lan, Chunbo Chen
BACKGROUND: Various special techniques for blind bedside transpyloric tube placement have been introduced into clinical practice. However, transpyloric spiral tube placement facilitated by a blind bedside method has not yet been reported. The objective of this prospective study was to evaluate the safety and efficiency of blind bedside postpyloric placement of a spiral tube as a rescue therapy subsequent to failed spontaneous transpyloric migration in critically ill patients. METHODS: This prospective, tricentric, observational study was conducted in the intensive care units (ICUs) of three tertiary hospitals...
September 26, 2017: Critical Care: the Official Journal of the Critical Care Forum
https://www.readbyqxmd.com/read/28944996/negative-pressure-wound-therapy-promotes-muscle-derived-stem-cell-osteogenic-differentiation-through-mapk-pathway
#15
Hong Liu, Xun Zheng, Liang Chen, Chao Jian, Xiang Hu, Yong Zhao, Zonghuan Li, Aixi Yu
Negative pressure wound therapy (NPWT) has been revealed to be effective in the treatment of open fractures, although the underlying mechanism is not clear. This article aimed to investigate the effects of NPWT on muscle-derived stem cell (MDSC) osteoblastic differentiation and the related potential mechanism. The cell proliferation rate was substantially increased in NPWT-treated MDSCs in comparison with a static group for 3 days. There was no observable effect on the apoptosis of MDSC treated with NPWT compared with the control group for 3 days...
September 25, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28937873/effect-of-parathyroidectomy-on-osteopontin-and-undercarboxylated-osteocalcin-in-patients-with-primary-hyperparathyroidism
#16
Raelene E Maser, M James Lenhard, Ryan T Pohlig, P Babu Balagopal, Raafat Abdel-Misih
PURPOSE: Surgical treatment for primary hyperparathyroidism (PHPT) improves bone metabolism. Osteocalcin (OC) and its undercarboxylated form (ucOC) are associated with bone and energy metabolism. Osteopontin (OPN), a multifunctional protein expressed in bone, is involved in resorption, along with β-carboxyl-terminal cross-linking telopeptide of type 1 collagen (β-CTX), and osteoprotegerin (OPG). Our aim was to investigate these biomarkers of bone metabolism in patients with PHPT. METHODS: We examined 30 individuals with PHPT, in a clinical research facility, before and 1 month following parathyroidectomy...
September 22, 2017: Endocrine Research
https://www.readbyqxmd.com/read/28935249/osteopontin-plays-a-unique-role-in-resistance-of-cd34-cd123-human-leukemia-cell-lines-kg1a-to-parthenolide
#17
Saeed Mohammadi, Mahdi Zahedpanah, Seyed Hamidollah Ghaffari, Mojgan Shaiegan, Mohsen Nikbakht, Mahin Nikugoftar
OBJECTIVES: To determine if parthenolide (PTL) is cytotoxic for leukemia-like KG1a cells and if it involves in certain molecular-mediated resistance, especially osteopontin (OPN). METHODS: PTL/daunorubicin (DNR)-treated KG1a cells were examined for viability using MTT and colony-formation assay, and stained for apoptosis using AV/PI. The gene and protein expression were evaluated by qReal-time PCR and Western blotting analysis, respectively. OPN gene was inhibited by OPN siRNA...
September 19, 2017: Life Sciences
https://www.readbyqxmd.com/read/28934886/occurrence-of-abdominal-bulging-and-hernia-after-open-partial-nephrectomy-a-retrospective-cohort-study
#18
Aapo Inkiläinen, Johan Styrke, Börje Ljungberg, Karin Strigård
OBJECTIVE: Abdominal bulging and incisional hernia are known sequelae after open partial nephrectomy (OPN) via a flank incision. Precise rates are not known. The aims of this study were to determine the rates of bulging and hernia after OPN, and to examine potential risk factors. MATERIALS AND METHODS: A retrospective review was undertaken of 197 consecutive patients operated on with OPN via a flank incision between 2004 and 2014. After exclusion, 184 patients remained...
September 22, 2017: Scandinavian Journal of Urology
https://www.readbyqxmd.com/read/28926103/fam20c-regulates-osteoblast-behaviors-and-intracellular-signaling-pathways-in-a-cell-autonomous-manner
#19
Chao Liu, Hua Zhang, Priyam Jani, Xiaofang Wang, Yongbo Lu, Nan Li, Jing Xiao, Chunlin Qin
Recent studies indicate that Family with sequence similarity 20 member C (FAM20C) catalyzes the phosphorylation of secreted proteins, and participates in a variety of biological processes, including cell proliferation, migration, mineralization and phosphate homeostasis. To explore the local influences of FAM20C on osteoblast, Fam20c-deficient osteoblasts were generated by treating the immortalized Fam20c(f/f) osteoblasts with CMV-Cre-IRES-EGFP lentivirus. Compared with the normal Fam20c(f/f) osteoblasts, the expression of Bone sialoprotein (Bsp), Osteocalcin (Ocn), Fibroblast growth factor 23 (Fgf23) and transcription factors that promote osteoblast maturation were up-regulated in the Fam20c-deficient osteoblasts...
September 19, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28922687/comparison-of-perioperative-renal-and-oncologic-outcomes-in-robotic-assisted-versus-open-partial-nephrectomy
#20
Jo-Lynn Tan, Mark Frydenberg, Jeremy Grummet, Uri Hanegbi, Ross Snow, Sarah Mann, Kirobel Begashaw, Daniel Moon
BACKGROUND: To compare perioperative, renal and oncological outcomes after robotic-assisted partial nephrectomy (RAPN) versus open partial nephrectomy (OPN) for the treatment of renal tumours. METHODS: All partial nephrectomies performed at a Metropolitan Urology Centre between 2010 and 2016 were analysed. Baseline data was collected for patient demographics, tumour characteristics (tumour size, laterality and polarity, RENAL scores), and perioperative variables (e...
September 18, 2017: ANZ Journal of Surgery
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