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https://www.readbyqxmd.com/read/28320084/lncrna-meg3-inhibited-osteogenic-differentiation-of-bone-marrow-mesenchymal-stem-cells-from-postmenopausal-osteoporosis-by-targeting-mir-133a-3p
#1
Qiujun Wang, Ying Li, Yuanxia Zhang, Lan Ma, Lin Lin, Jia Meng, Lihong Jiang, Liping Wang, Ping Zhou, Yina Zhang
BACKGROUND AND AIMS: Long non-coding RNA (lncRNA) MEG3 has proven to be an important regulator involved in the pathogenesis and development of various human diseases. However, the functional involvement of MEG3 in postmenopausal osteoporosis (PMOP) and its mechanism is still unclear. METHODS: Bone marrow mesenchymal stem cells (BMSCs) were isolated and cultured from mouse pathologic models and patients with PMOP, respectively. The expression of MEG3 and miR-133a-3p in BMSCs was detected using qRT-PCR...
March 14, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28304382/inhibition-of-cathepsin-k-promotes-osseointegration-of-titanium-implants-in-ovariectomised-rats
#2
Chun Yi, Ke-Yi Hao, Ting Ma, Ye Lin, Xi-Yuan Ge, Yu Zhang
The bone mineral deficiency in osteoporosis poses a threat to the long-term outcomes of endosseous implants. The inhibitors of cathepsin K (CatK) significantly affect bone turnover, bone mineral density (BMD) and bone strength in the patients with osteoporosis. Therefore, we hypothesised that the application of a CatK inhibitor (CatKI) could increase the osseointegration of endosseous implants under osteoporotic conditions. Odanacatib (ODN), a highly selective CatKI, was chosen as the experimental drug. Sixteen rats were randomised into 4 groups: sham, ovariectomy (OVX) with vehicle, OVX with low-dose ODN (5 mg/kg) and OVX with high-dose ODN (30 mg/kg)...
March 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28303318/bone-alkaline-phosphatase-and-tartrate-resistant-acid-phosphatase-potential-co-regulators-of-bone-mineralization
#3
Cecilia Halling Linder, Barbro Ek-Rylander, Michael Krumpel, Maria Norgård, Sonoko Narisawa, José Luis Millán, Göran Andersson, Per Magnusson
Phosphorylated osteopontin (OPN) inhibits hydroxyapatite crystal formation and growth, and bone alkaline phosphatase (BALP) promotes extracellular mineralization via the release of inorganic phosphate from the mineralization inhibitor inorganic pyrophosphate (PPi). Tartrate-resistant acid phosphatase (TRAP), produced by osteoclasts, osteoblasts, and osteocytes, exhibits potent phosphatase activity towards OPN; however, its potential capacity as a regulator of mineralization has not previously been addressed...
March 16, 2017: Calcified Tissue International
https://www.readbyqxmd.com/read/28302667/novel-device-for-application-of-continuous-mechanical-tensile-strain-to-mammalian-cells
#4
Satoshi Wada, Hiroyuki Kanzaki, Tsuyoshi Narimiya, Yoshiki Nakamura
During orthodontic tooth movement, the periodontal ligament (PDL) is exposed to continuous mechanical strain. However, many researchers have applied cyclic tensile strain, not continuous tensile strain, to PDL cells in vitro, because there has been no adequate device to apply continuous tensile strain to cultured cells. In this study, we contrived a novel device designed to apply continuous tensile strain to cells in culture. The continuous tensile strain was applied to human immortalized periodontal ligament cell line (HPL cells) and the cytoskeletal structures of HPL cells were examined by immunohistochemistry...
March 16, 2017: Biology Open
https://www.readbyqxmd.com/read/28302392/osteopontin-regulates-the-proliferation-of-rat-aortic-smooth-muscle-cells-in-response-to-gingipains-treatment
#5
Chong Cao, Xin Luo, Xiaowei Ji, Yao Wang, Yuan Zhang, Pengtao Zhang, Liangjun Zhong
OBJECTIVE: The present study aimed to explore the possible effects of osteopontin (OPN) in the proliferation of rat aortic smooth muscle cells (RASMCs) stimulated by gingipains. METHODS: The proliferation of RASMCs in response to active gingipains treatment was evaluated by CCK-8 assay. OPN siRNA was designed, constructed and transfected into RASMCs at different concentrations. The cell cycle of RASMCs was analyzed by flow cytometry. OPN, α-SMA and calponin expression were examined by real-time PCR and western blot analysis...
March 13, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28301220/periostin-promotes-migration-proliferation-and-differentiation-of-human-periodontal-ligament-mesenchymal-stem-cells
#6
Ziqiang Wu, Wenyong Dai, Pei Wang, Xiaozhen Zhang, Yi Tang, Lin Liu, Qiaona Wang, Ming Li, Chunbo Tang
PURPOSE: Periostin (POSTN) is critical to bone and dental tissue morphogenesis, postnatal development, and maintenance, however, its roles in tissue repair and regeneration mediated by human periodontal ligament mesenchymal stem cells (PDLSCs) remain unclear. The present study was designed to evaluate the effects of POSTN on hPDLSCs in vitro. MATERIALS AND METHODS: hPDLSCs were isolated and characterized by their expression of the cell surface markers CD44, CD90, CD105, CD34, and CD45...
March 16, 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/28300837/a-cd44v-subpopulation-of-breast-cancer-stem-like-cells-with-enhanced-lung-metastasis-capacity
#7
Jing Hu, Gang Li, Peiyuan Zhang, Xueqian Zhuang, Guohong Hu
Cancer stem-like cells (CSCs) are a subpopulation of cancer cells responsible for tumor growth, and recent evidence suggests that CSCs also contribute to cancer metastasis. However, the heterogeneity of CSCs in metastasis capacities is still unclear in breast cancer. Here we show that among the CD24(-)/CD44(+) breast CSCs, a subset expressing the variant isoform of CD44 (CD44v) displays significantly higher capacity of lung metastasis than that expressing the standard CD44 isoform CD44s. Increasing or reducing the CD44v/CD44s ratio of breast cancer cells by regulating the expression of epithelial splicing regulatory protein 1 (ESRP1) leads to promotion or suppression of lung metastasis without influencing cancer cell stemness...
March 16, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28296922/the-potential-role-of-osteopontin-in-the-maintenance-of-commensal-bacteria-homeostasis-in-the-intestine
#8
Koyu Ito, Akira Nakajima, Yuji Fukushima, Keiichiro Suzuki, Keiko Sakamoto, Yoko Hamazaki, Kouetsu Ogasawara, Nagahiro Minato, Masakazu Hattori
Osteopontin (Opn), a multifunctional extracellular matrix protein, is implicated in the pathogenesis of various inflammatory disorders. Under physiologic conditions, its expression is restricted to certain tissues including bone and kidney tubule. However, cellular activation during disease development induces Opn expression in various immune cells. In this study, using Opn-EGFP knock-in (KI) mice we found that CD8α+ T cells in the intestinal tissues, including Peyer's patch, lamina propria and epithelium, express Opn under steady state conditions...
2017: PloS One
https://www.readbyqxmd.com/read/28292602/investigation-of-human-dental-pulp-cells-on-a-potential-injectable-poly-lactic-co-glycolic-acid-microsphere-scaffold
#9
Huiru Zou, Guanhua Wang, Fang Song, Xudong Shi
INTRODUCTION: Poly(lactic-co-glycolic acid) (PLGA) has been extensively explored in the tissue engineering field with good biocompatibility and biodegradability. PLGA microspheres' injectable potency makes it highly desirable in dentin-pulp complex regeneration. Therefore, we investigated the cell adhesion, proliferation, odontogenic differentiation, and matrix mineralization of human dental pulp cells (HDPCs) on a PLGA microsphere scaffold. We hypothesized that this scaffold might be suitable for dentin-pulp complex regeneration...
March 11, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28292315/anatomical-organization-and-neural-pathways-of-the-ovarian-plexus-nerve-in-rats
#10
César F Pastelín, Nora H Rosas, Leticia Morales-Ledesma, Rosa Linares, Roberto Domínguez, Carolina Morán
BACKGROUND: In this work, a detailed anatomical description of the ovarian plexus nerve (OPN) in rats is presented. The distribution of the OPN was analyzed by gross anatomy; the features of the superior mesenteric ganglion (SMG) were determined by histological studies; and the localization of the postganglionic neurons innervating the ovary were identified with retrograde tracer. We studied 19 adult cyclic rats of the CIIZ-V strain. RESULTS: We found that the right OPN originates from the celiac ganglion, the lumbar ganglion of the sympathetic trunk (LGST) and the SMG...
March 14, 2017: Journal of Ovarian Research
https://www.readbyqxmd.com/read/28291627/diagnostic-accuracy-of-osteopontin-plus-alpha-fetoprotein-in-the-hepatocellular-carcinoma-a-meta-analysis
#11
Jinwan Li, Xiaoli Chen, Meiyu Dai, Shifeng Huang, Jingfan Chen, Shengming Dai
OBJECTIVE: Osteopontin (OPN) has been reported as a potential biomarker for diagnosis of hepatocellular carcinoma (HCC) in many inconsistent results. This study demonstrates a systematic meta-analysis for the evaluation on diagnostic accuracy of serum or plasma OPN and alpha-fetoprotein (AFP) alone and combined assays for HCC. METHODS: Relevant literatures were searched in PubMed up to August 2016. The quality of each study was evaluated by QUADAS-2 (quality assessment for studies of diagnostic accuracy)...
March 10, 2017: Clinics and Research in Hepatology and Gastroenterology
https://www.readbyqxmd.com/read/28291358/phenolic-polymer-solvation-in-water-and-ethylene-glycol-i-molecular-dynamics-simulations
#12
Eric W Bucholz, Justin B Haskins, Joshua D Monk, Charles W Bauschlicher, John W Lawson
Interactions between pre-cured phenolic polymer chains and a solvent have a significant impact on the structure and properties of the final post-cured phenolic resin. Developing an understanding of the nature of these interactions is important and will aid in the selection of the proper solvent that will lead to the desired final product. Here, we investigate the role of phenolic chain structure and solvent type on the overall solvation performance of the system through molecular dynamics simulations. Two types of solvents are considered, ethylene glycol (EGL) and H2O...
March 14, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28280797/evaluation-of-two-endometriosis-models-by-transplantation-of-human-endometrial-tissue-fragments-and-human-endometrial-mesenchymal-cells
#13
Mina Jafarabadi, Mojdeh Salehnia, Rana Sadafi
BACKGROUND: The animal models of endometriosis could be a valuable alternative tool for clarifying the etiology of endometriosis. OBJECTIVE: In this study two endometriosis models at the morphological and molecular levels was evaluated and compared. MATERIALS AND METHODS: The human endometrial tissues were cut into small fragments then they were randomly considered for transplantation into γ irradiated mice as model A; or they were isolated and cultured up to fourth passages...
January 2017: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/28274194/the-combined-effects-of-nanotopography-and-sr-ion-for-enhanced-osteogenic-activity-of-bone-marrow-mesenchymal-stem-cells-bmscs
#14
Dandan Hu, Kai Li, Youtao Xie, Houhua Pan, Jun Zhao, Liping Huang, Xuebin Zheng
Both surface topography and chemistry have a significant influence on the biological performance of orthopedic implant coatings. In our study, a surface modification strategy embodying bioactive trace element incorporation and nanotopography construction was employed to enhance the osteogenic activity of calcium silicate (Ca-Si) coatings. We developed strontium-loaded nanolayer on plasma sprayed Ca-Si (CS) coating via hydrothermal treatment which was denoted as Sr-NT-CS. The original CS coating and the CS coating modified with similar nanotopography (NT-CS) were studied in parallel...
March 2017: Journal of Biomaterials Applications
https://www.readbyqxmd.com/read/28272636/the-correlation-between-osteopontin-adsorption-and-cell-adhesion-to-mixed-self-assembled-monolayers-of-varying-charges-and-wettability
#15
Lijing Hao, Tianjie Li, Fan Yang, Naru Zhao, Fuzhai Cui, Xuetao Shi, Chang Du, Yingjun Wang
Osteopontin (OPN) is a key mediator of cell interactions with biomaterials. However, few studies have been dedicated to studying cell adhesion on OPN-adsorbed substrates with controlled charge and wettability. Here, amino-carboxyl (NH2/COOH) and hydroxyl-methyl (OH/CH3) mixed self-assembled monolayers (SAMs) of varying charges and wettability, respectively, were used as controllable model surfaces to study OPN adsorption and subsequent mesenchymal stem cell (MSC) adhesion. The amount of OPN adsorbed onto the NH2/COOH mixed SAMs appeared to monotonically depend on the surface charge, whereas only a moderately hydrophilic surface was conducive to OPN adsorption on OH/CH3 mixed SAMs...
March 8, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28267132/pentraxin3-as-a-new-indicator-of-cardiovascular-related-death-in-patients-with-advanced-chronic-kidney-disease
#16
Marcin Krzanowski, Katarzyna Krzanowska, Mariusz Gajda, Paulina Dumnicka, Artur Dziewierz, Karolina Woziwodzka, Jan A Litwin, Władysław Sułowicz
INTRODUCTION    Pentraxin3 (PTX3) play an important role in the inflammatory response, taking part in recognizing pathogens and damaged tissues. OBJECTIVES    The aim of the study was to assess the relationship between PTX3 levels and all-cause and cardiovascular (CV) mortality in chronic kidney disease (CKD) patients during five-year observation period.  PATIENTS AND METHODS    The study comprised 78 patients (51 hemodialyzed, 27 predialysis). The examined parameters included PTX3, calcium, phosphate, iPTH, interleukin-6 (IL-6), fibroblast growth factor 23 (FGF-23), osteopontin (OPN), osteoprotegerin (OPG), osteocalcin (OC), osteopontin (OPN), osteoprotegerin (OPG), osteocalcin, tumor necrosis factor receptor II (TNF-R II), transforming growth factor-β (TGF-β), hepatocyte growth factor (HGF), stromal cell-derived factor α (SDF1α), and thrombomodulin (TM)...
February 27, 2017: Pol Arch Intern Med
https://www.readbyqxmd.com/read/28266351/biofabrication-of-a-co-culture-system-in-an-osteoid-like-hydrogel-matrix
#17
Tobias Zehnder, Aldo Boccaccini, Rainer Detsch
Biofabrication aims to develop functional, biological constructs using automated processes (additive manufacturing, AM) involving different cell types and biomaterials [1]. As bone tissue is based on the crosstalk between osteoblasts and osteoclasts at least, evaluating cell-cell and cell-material interactions is of interest to understand bone remodeling. There is increasing interest in the role of osteoclasts not only considering bone resorption, but also their influence on the proliferation, migration and differentiation of osteoblasts...
March 7, 2017: Biofabrication
https://www.readbyqxmd.com/read/28266021/gingival-spheroids-possess-multilineage-differentiation-potential
#18
Rajasekaran Subbarayan, Dinesh Murugan Girija, Suresh Ranga Rao
Recently studies have demonstrated HGMSCs as ideal candidates for regenerative study. Interestingly we found that HGMSCs derived spheroids are more potent and maintain the properties of stemness convincingly compared to conventional culture methods. During the culture, GMSCs instinctively accumulated into spheroids and display multipotent STRO-1 and Vimentin-positive cells. Reduced phenotypic expression of CD73, CD105 and, elevated expression STRO-1 and CD-34. Pluripotent nature of S-GMSCs putatively shown the expression of OCT4A, NANOG, SOX-2, SSEA4, TRA-1-60, and TRA-181...
March 7, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28259975/gene-expression-profiling-in-bladder-cancer-identifies-potential-therapeutic-targets
#19
Syed A Hussain, Daniel H Palmer, Wing-Kin Syn, Joseph J Sacco, Richard M D Greensmith, Taha Elmetwali, Vijay Aachi, Bryony H Lloyd, Puthen V Jithesh, John Arrand, Darren Barton, Jawaher Ansari, D Ross Sibson, Nicholas D James
Despite advances in management, bladder cancer remains a major cause of cancer related complications. Characterisation of gene expression patterns in bladder cancer allows the identification of pathways involved in its pathogenesis, and may stimulate the development of novel therapies targeting these pathways. Between 2004 and 2005, cystoscopic bladder biopsies were obtained from 19 patients and 11 controls. These were subjected to whole transcript-based microarray analysis. Unsupervised hierarchical clustering was used to identify samples with similar expression profiles...
March 2, 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28256244/oscillatory-fluid-flow-induces-the-osteogenic-lineage-commitment-of-mesenchymal-stem-cells-the-effect-of-shear-stress-magnitude-frequency-and-duration
#20
Elena Stavenschi, Marie-Noelle Labour, David A Hoey
A potent regulator of bone anabolism is physical loading. However, it is currently unclear whether physical stimuli such as fluid shear within the marrow cavity is sufficient to directly drive the osteogenic lineage commitment of resident mesenchymal stem cells (MSC). Therefore, the objective of the study is to employ a systematic analysis of oscillatory fluid flow (OFF) parameters predicted to occur in vivo on early MSC osteogenic responses and late stage lineage commitment. MSCs were exposed to OFF of 1Pa, 2Pa and 5Pa magnitudes at frequencies of 0...
February 21, 2017: Journal of Biomechanics
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