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https://www.readbyqxmd.com/read/28229307/investigation-of-osteogenic-activity-of-primary-rabbit-periosteal-cells-stimulated-by-multi-axial-tensile-strain
#1
Chih-Hao Chiu, Jun-Liang Liu, Chih-Hsuan Chang, Kin Fong Lei, Alvin Chao-Yu Chen
Periosteum-derived cells was indicated to respond to mechanical force and have stem cell potential capable of differentiating into multiple tissue. Investigation of osteogenic activity under mechanical stimulation is important to understand the therapeutic conditions of fracture healing. In this work, a cell culture platform was developed for respectively providing isotropic and anisotropic axial strain. Primary rabbit periosteal cells were isolated and cultured in the chamber. Multi-axial tensile strain was received and osteogenic activity was investigated by mRNA expressions of CBFA1 and OPN...
March 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28210757/sildenafil-a-phosphodiesterase-type-5-inhibitor-downregulates-osteopontin-in-human-peripheral-blood-mononuclear-cells
#2
Beata Kaleta, Agnieszka Boguska
The aim of this study was to investigate the ability of sildenafil to regulate osteopontin (OPN) gene and protein in peripheral blood mononuclear cells (PBMCs) from healthy blood donors. OPN is expressed by a wide variety of cell types, including immune cells. OPN functions are linked to various physiological and pathological conditions. Sildenafil is a selective inhibitor of type 5 phosphodiesterase. Sildenafil has recently been found to have immunomodulatory effects in animal models and in studies performed in humans...
February 16, 2017: Archivum Immunologiae et Therapiae Experimentalis
https://www.readbyqxmd.com/read/28202997/-a-novel-tissue-engineered-bone-constructed-by-using-human-adipose-derived-stem-cells-and-biomimetic-calcium-phosphate-scaffold-coprecipitated-with-bone-morphogenetic-protein-2
#3
W R Jiang, X Zhang, Y S Liu, G Wu, Y J Ge, Y S Zhou
OBJECTIVE: To construct a novel biomimetic calcium phosphate (BioCaP) scaffold loaded with bone morphogenetic protein-2 (BMP-2), and to investigate its role in the osteogenesis of human adipose-derived stem cells (hASCs) in vitro and in vivo. METHODS: The BioCaP scaffold coprecipitated with BMP-2 (BMP-2-BioCaP) was constructed in this study. Field emission scanning electron microscopy (SEM) was used to analyze the morphology of the surfaces. The release kinetics was measured to evaluate the slow-release characteristics in vitro...
February 18, 2017: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/28196866/sglt2-expression-is-increased-in-human-diabetic-nephropathy-sglt2-inhibition-decreases-renal-lipid-accumulation-inflammation-and-the-development-of-nephropathy-in-diabetic-mice
#4
Xiaoxin X Wang, Jonathan Levi, Yuhuan Luo, Komuraiah Myakala, Michal Herman-Edelstein, Liru Qiu, Dong Wang, Yingqiong Peng, Almut Grenz, Scott Lucia, Evgenia Dobrinskikh, Vivette D D'Agati, Hermann Koepsell, Jeffrey B Kopp, Avi Rosenberg, Moshe Levi
There is very limited human renal sodium gradient dependent glucose transporter protein SGLT2 mRNA and protein expression data reported in the literature. Aim 1 of this study was to determine SGLT2 mRNA and protein levels in human and animal models of diabetic nephropathy. We have found that the expression of SGLT2 mRNA and protein is increased in renal biopsies from human subjects with diabetic nephropathy. This is in contrast to db-db mice which had no changes in renal SGLT-2 protein expression. Furthermore, the effect of SGLT2 inhibition on renal lipid content and inflammation is not known...
February 14, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28195408/microrna-22-promoted-osteogenic-differentiation-of-human-periodontal-ligament-stem-cells-by-targeting-hdac6
#5
Guang-Qi Yan, Xue Wang, Fan Yang, Min-Liang Yang, Guo-Kun Wang, Qing Zhou
Stem cells transplantation is a promising therapy strategy for accelerating periodontal regeneration and reconstruction. Genetic modification could induce stem cells directional differentiation to facilitate recovery of physiological functions. In this study, we investigated the role and mechanism of miR-22 on human periodontal ligament stem cells (PDLSCs). Firstly, a cellular model of osteogenic differentiation was first established by osteogenic inductive cocktail. Real-time PCR determined that expression of miR-22 was significantly increased during PDLSCs osteogenic differentiation...
February 13, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28194187/effect-of-hypoxia-on-the-differentiation-and-the-self-renewal-of-metanephrogenic-mesenchymal-stem-cells
#6
Shaopeng Liu, Nana Song, Jianqiang He, Xiaofang Yu, Jia Guo, Xiaoyan Jiao, Xiaoqiang Ding, Jie Teng
Hypoxia is an important and influential factor in development. The embryonic kidney is exposed to a hypoxic environment throughout its development. The Wnt/β-catenin pathway plays vital roles in the differentiation and self-renewal of metanephrogenic mesenchymal stem cells (MMSCs) from which the kidney is derived. Thus, we hypothesized that hypoxia can regulate the differentiation and pluripotency of MMSCs through the Wnt/β-catenin pathway. To test this hypothesis, MMSCs from rats at embryonic day 18.5 were cultured in normoxic (21% O2) and hypoxic (1% O2) conditions...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28194185/osteopontin-relation-between-adipose-tissue-and-bone-homeostasis
#7
REVIEW
Carolina De Fusco, Antonietta Messina, Vincenzo Monda, Emanuela Viggiano, Fiorenzo Moscatelli, Anna Valenzano, Teresa Esposito, Chieffi Sergio, Giuseppe Cibelli, Marcellino Monda, Giovanni Messina
Osteopontin (OPN) is a multifunctional protein mainly associated with bone metabolism and remodeling. Besides its physiological functions, OPN is implicated in the pathogenesis of a variety of disease states, such as obesity and osteoporosis. Importantly, during the last decades obesity and osteoporosis have become among the main threats to health worldwide. Because OPN is a protein principally expressed in cells with multifaceted effects on bone morphogenesis and remodeling and because it seems to be one of the most overexpressed genes in the adipose tissue of the obese contributing to osteoporosis, this mini review will highlight recent insights about relation between adipose tissue and bone homeostasis...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28193231/association-of-osteopontin-with-specific-prognostic-factors-and-survival-in-adjuvant-breast-cancer-trials-of-the-hellenic-cooperative-oncology-group
#8
Amanda Psyrri, Konstantine T Kalogeras, Ralph M Wirtz, George Kouvatseas, Georgia Karayannopoulou, Anna Goussia, Flora Zagouri, Elke Veltrup, Eleni Timotheadou, Helen Gogas, Angelos Koutras, Georgios Lazaridis, Christos Christodoulou, George Pentheroudakis, Panagiota Economopoulou, Apostolos Laskarakis, Petroula Arapantoni-Dadioti, Anna Batistatou, Maria Sotiropoulou, Gerasimos Aravantinos, Pavlos Papakostas, Paris Kosmidis, Dimitrios Pectasides, George Fountzilas
BACKGROUND: The shift towards an earlier diagnosis of breast cancer (BC) highlights the need for biomarkers that would identify patients at risk for relapse and metastatic spread and indicate the potential value of additional treatment strategies. Osteopontin (OPN) is a matricellular protein that has been suggested to be a potential biomarker in BC. In the present study, we used archived BC patient samples to assess the clinical utility of OPN. METHODS: Formalin-fixed paraffin-embedded tumor tissue samples from 975 patients were collected from two large phase III randomized adjuvant chemotherapy trials (HE10/97 and HE10/00) that included patients with high risk BC...
February 13, 2017: Journal of Translational Medicine
https://www.readbyqxmd.com/read/28192120/osteopontin-attenuates-acute-gastrointestinal-graft-versus-host-disease-by-preventing-apoptosis-of-intestinal-epithelial-cells
#9
Kentaro Kawakami, Naoki Minami, Minoru Matsuura, Tomoya Iida, Takahiko Toyonaga, Kanna Nagaishi, Yoshiaki Arimura, Mineko Fujimiya, Toshimitsu Uede, Hiroshi Nakase
BACKGROUND AND AIMS: Acute graft-versus-host disease (GVHD) is a major complication after allogeneic hematopoietic stem cell transplantation, which often targets gastrointestinal (GI) tract. Osteopontin (OPN) plays an important physiological role in the efficient development of Th1 immune responses and cell survival by inhibiting apoptosis. The role of OPN in acute GI-GVHD is poorly understood. In the present study, we investigated the role of OPN in donor T cells in the pathogenicity of acute GI-GVHD...
February 9, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28183637/surface-modification-of-tio2-nanotubes-with-osteogenic-growth-peptide-to-enhance-osteoblast-differentiation
#10
Min Lai, Ziyang Jin, Zhiguo Su
To investigate the influence of surface-biofunctionalized substrates on osteoblast behavior, a layer of aligned TiO2 nanotubes with a diameter of around 70nm was fabricated on titanium surface by anodization, and then osteogenic growth peptide (OGP) was conjugated onto TiO2 nanotubes through the intermediate layer of polydopamine. The morphology, composition and wettability of different surfaces were characterized by field-emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS) and contact angle measurements, respectively...
April 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28183618/evaluation-of-the-osteo-inductive-potential-of-hollow-three-dimensional-magnesium-strontium-substitutes-for-the-bone-grafting-application
#11
Mei Li, Xuan Yang, Weidan Wang, Yu Zhang, Peng Wan, Ke Yang, Yong Han
Regeneration of bone defects is a clinical challenge that usually necessitates bone grafting materials. Limited bone supply and donor site morbidity limited the application of autografting, and improved biomaterials are needed to match the performance of autografts. Osteoinductive materials would be the perfect candidates for achieving this task. Strontium (Sr) is known to encourage bone formation and also prevent osteoporosis. Such twin requirements have motivated researchers to develop Sr-substituted biomaterials for orthopedic applications...
April 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28182765/rapamycin-sodium-hyaluronate-binding-on-nano-hydroxyapatite-coated-titanium-surface-improves-mc3t3-e1-osteogenesis
#12
Chao Liu, Jian Yong Dong, Lin Lin Yue, Shao Hua Liu, Yi Wan, Hong Liu, Wan Ye Tan, Qian Qian Guo, Dong Zhang
Endosseous titanium (Ti) implant failure due to poor biocompatibility of implant surface remains a major problem for osseointegration. Improving the topography of Ti surface may enhance osseointegration, however, the mechanism remains unknown. To investigate the effect of modified Ti surface on osteogenesis, we loaded rapamycin (RA) onto nano-hydroxyapatite (HAp) coated Ti surface which was acid-etched, alkali-heated and HAp coated sequentially. Sodium hyaluronate (SH) was employed as an intermediate layer for the load of RA, and a steady release rate of RA was maintained...
2017: PloS One
https://www.readbyqxmd.com/read/28181432/mimicking-hierarchical-complexity-of-the-osteochondral-interface-using-electrospun-silk-bioactive-glass-composites
#13
Joseph Christakiran M, Philip J T Reardon, Rocktotpal Konwarh, Jonathan C Knowles, Biman B Mandal
The anatomical complexity and slow regeneration capacity of hyaline cartilage at the osteochondral interface pose a great challenge in the repair of osteochondral defects (OCD). In this study, we utilized the processing feasibility offered by the sol derived 70S bioactive glass and silk fibroin (mulberry Bombyx mori and endemic Indian non-mulberry Antheraea assama), in fabricating a well-integrated, biomimetic scaffolding matrix with a coherent interface. Differences in surface properties such as wettability and amorphousness between the two silk groups resulted in profound variations in cell attachment and extracellular matrix protein deposition...
February 22, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28167124/p38-mapk-signaling-pathway-mediates-angiotensin-ii-induced-mir143-145-gene-cluster-downregulation-during-aortic-dissection-formation
#14
Bowen Li, Zhiwei Wang, Zhipeng Hu, Min Zhang, Zongli Ren, Zhen Zhou, Jizhen Huang, Xiaoping Hu
BACKGROUND: We endeavored to prove that angiotensin II (Ang II) regulates both the expression of micro-RNA143/145 (miR143/145) and differentiation of vascular smooth muscle cells (VSMCs) during the formation of aortic dissection (AD). We also studied the contribution of p38 mitogen-activated protein kinase (MAPK) signaling pathway toward this process. METHODS: Ascending aortic tissues were harvested from the patients with AD and organ donors. Tissues were immunostained with labeled antibodies targeting p38 MAPK, phospho-p38 MAPK, alpha-smooth muscle actin (α-SMA), and osteopontin (OPN)...
February 4, 2017: Annals of Vascular Surgery
https://www.readbyqxmd.com/read/28148719/matricellular-proteins-tune-myeloid-derived-suppressor-cell-recruitment-and-function-in-breast-cancer
#15
REVIEW
Claudia Chiodoni, Sabina Sangaletti, Mario P Colombo
Solid tumor progression is often associated with the expansion of myeloid-derived suppressor cells (MDSCs), a heterogeneous population of immature myeloid cells that actively foster tumor growth and metastatic dissemination through a plethora of mechanisms, including, but not limited to, their major suppressive activity on the immune response. Indeed, MDSCs may sustain tumor progression by dynamically remodeling the tumor microenvironment through the production of angiogenic factors and metalloproteases, by helping the establishment of a premetastatic niche, and by promoting stemness and epithelial-to-mesenchymal transition (EMT) features in tumor cells...
February 1, 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/28146138/comparison-the-relationship-between-the-levels-of-insulin-resistance-hs-crp-percentage-of-body-fat-and-serum-osteoprotegerin-receptor-activator-of-nuclear-factor-%C3%AE%C2%BA%C3%AE-ligand-in-prediabetic-patients
#16
Oktay Bilgir, Mehmet Yavuz, Ferda Bilgir, Ozden Y Akan, Aslı G Bayindir, Mehmet Calan, Giray Bozkaya, Arif Yuksel
: BACKGROUND Receptor activator of nuclear factor-kappaB ligand (RANKL) and osteoprotegerin (OPN) are soluble members of the tumor necrosis factor superfamily. Growing evidence suggest that there is link between inflammation, insulin resistance and OPG, sRANKL. We aimed to ascertain whether OPG and sRANKL levels are altered in prediabetic subjects and there is association between OPG, sRANKL and metabolic parameters. MATERIAL AND METHODS: 40 prediabetic subjects and 40 age- and BMI-matched controls were recruited for this cross-sectional study...
January 31, 2017: Minerva Endocrinologica
https://www.readbyqxmd.com/read/28136130/integrin-%C3%AE-8-is-abundant-in-human-rat-and-mouse-trophoblasts
#17
Sebastian Herdl, Hanna Huebner, Gudrun Volkert, Ines Marek, Carlos Menendez-Castro, Stephanie C Noegel, Matthias Ruebner, Wolfgang Rascher, Andrea Hartner, Fabian B Fahlbusch
OBJECTIVE: Integrins exert regulatory functions in placentogenesis. Null mutation of certain integrin α subunits leads to placental defects with subsequent fetal growth restriction or embryonic lethality in mice. So far, the placental role of α8 integrin remains to be determined. METHODS: Localization of α8 integrin and its ligands, fibronectin (FN) and osteopontin (OPN), was studied by immunohistochemistry in human, rat, and mouse placenta. The vascularization of the placental labyrinth layer of α8 integrin-deficient mice was determined by CD31 staining...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/28134597/endogenous-bone-morphogenetic-protein-2-plays-a-role-in-vascular-smooth-muscle-cell-calcification-induced-by-interleukin-6-in-vitro
#18
Mingshu Sun, Qing Chang, Miaomiao Xin, Qian Wang, Hua Li, Jiaqi Qian
Systemic inflammation is involved in vascular calcification and cardiovascular disease which is the leading cause of mortality in rheumatoid arthritis (RA). A high level of serum interleukin (IL)-6 plays a key role in local and systemic inflammation in RA. However, the underlying mechanisms remain unclear. We established a human umbilical artery smooth muscle cell (HUASMC) culturing method to investigate the possible role of IL-6 on vascular calcification. HUASMCs were obtained from umbilical arteries of healthy neonates...
January 1, 2017: International Journal of Immunopathology and Pharmacology
https://www.readbyqxmd.com/read/28132802/the-ability-of-hesperidin-compared-to-that-of-insulin-for-preventing-osteoporosis-induced-by-type-i-diabetes-in-young-male-albino-rats-a-histological-and-biochemical-study
#19
Azza Saad Shehata, Mona Gomah Amer, Manal Reda Abd El-Haleem, Rehab Ahmed Karam
BACKGROUND: Patients with type I diabetes are at increased risk of osteoporosis even after insulin therapy in adult stage. This study was conducted to compare the efficacy of hesperidin (hesp) therapy versus that of insulin alone in the alleviation of osteoporosis arising from type I diabetes mellitus (T1DM) in young rats. MATERIALS AND METHODS: Hesperidin was administered orally to STZ-induced diabetes. The animals were evaluated morphologically and biochemically and compared with that received daily SC injections of long-acting insulin...
January 26, 2017: Experimental and Toxicologic Pathology: Official Journal of the Gesellschaft Für Toxikologische Pathologie
https://www.readbyqxmd.com/read/28128905/open-partial-nephrectomy-for-entirely-intraparenchymal-tumors-a-matched-case-control-study-of-oncologic-outcome-and-complication-rate
#20
Piotr Zapala, Bartosz Dybowski, Nina Miazek, Piotr Radziszewski
PURPOSE: To compare the oncologic and clinical outcomes for open partial nephrectomy (OPN) performed in patients with entirely intraparenchymal tumors versus casematched controls, with exophytic lesions. MATERIAL AND METHODS: Patients having undergone OPN between 2007 and 2012 were investigated. Exclusion criteria included patients with a benign tumor, advanced malignancy, malignancies other than renal cell carcinoma, end-stage renal failure, or 3 or more co-existing chronic diseases...
January 27, 2017: International Braz J Urol: Official Journal of the Brazilian Society of Urology
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