Bárbara P N Assis, Ana T Chaves, Daniela P Lage, Mariana M Cardoso, Isabela A G Pereira, Raquel S B Câmara, Camila S Freitas, Vívian T Martins, Fernanda Ludolf, Ana Laura G de Oliveira, João A Oliveira-da-Silva, Grasiele S V Tavares, Alexsandro S Galdino, Miguel A Chávez-Fumagalli, Ricardo A Machado-de-Ávila, Myron Christodoulides, Denise U Gonçalves, Lílian L Bueno, Ricardo T Fujiwara, Eduardo A F Coelho, Manoel Otávio da Costa Rocha
The diagnosis if leprosy is difficult, as it requires clinical expertise and sensitive laboratory tests. In this study, we develop a serological test for leprosy by using bioinformatics tools to identify specific B-cell epitopes from Mycobacterium leprae hypothetical proteins, which were used to construct a recombinant chimeric protein, M1. The synthetic peptides were obtained and showed good reactivity to detect leprosy patients, although the M1 chimera have showed sensitivity (Se) and specificity (Sp) values higher than 90...
May 3, 2024: Diagnostic Microbiology and Infectious Disease