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Askild L Holck, Kristian H Liland, Signe M Drømtorp, Mats Carlehög, Anette McLEOD
Ten percent of all strong-evidence foodborne outbreaks in the European Union are caused by Salmonella related to eggs and egg products. UV light may be used to decontaminate egg surfaces and reduce the risk of human salmonellosis infections. The efficiency of continuous UV-C (254 nm) and pulsed UV light for reducing the viability of Salmonella Enteritidis, Listeria monocytogenes, and enterohemorrhagic Escherichia coli on eggs was thoroughly compared. Bacterial cells were exposed to UV-C light at fluences from 0...
January 2018: Journal of Food Protection
Daniel Hernandez-Patlan, Bruno Solis-Cruz, Abraham Méndez-Albores, Juan D Latorre, Xochitl Hernandez-Velasco, Guillermo Tellez, Raquel López-Arellano
AIMS: To compare the conventional plating method versus a fluorometric method using PrestoBlue® as a dye by determining the antimicrobial activity of two organic acids and curcumin against Salmonella Enteritidis in an avian in vitro digestion model that simulates the crop, proventriculus and intestine. METHODS AND RESULTS: A concentration of 108 cfu ml-1 of S. Enteritidis was exposed to groups with different rates of ascorbic acid, boric acid and curcumin. Significant differences were observed when the means of the treatments were compared with the controls in the compartments that simulate the crop and intestine (P < 0...
December 7, 2017: Journal of Applied Microbiology
Qiuchun Li, Xin Wang, Kequan Yin, Yachen Hu, Haiyan Xu, Xiaolei Xie, Lijuan Xu, Xiao Fei, Xiang Chen, Xinan Jiao
Salmonella enterica serovar Enteritidis (S. Enteritidis) is one of the most prevalent serotypes in Salmonella isolated from poultry and the most commonly reported cause of human salmonellosis. In this study, we aimed to assess the genetic diversity of 329 S. Enteritidis strains isolated from different sources from 2009 to 2016 in China. Clustered regularly interspaced short palindromic repeat (CRISPR) typing was used to characterize these 262 chicken clinical isolates, 38 human isolates, 18 pig isolates, six duck isolates, three goose isolates and two isolates of unknown source...
November 28, 2017: International Journal of Food Microbiology
Jun Xu, Kazuasa Suita, Katsuya Okuno, Akiko Takaya, Tomoko Yamamoto, Emiko Isogai
The viable but non-culturable (VBNC) state is a remarkable survival mechanism in which cells exist in a physiologically inactive state. Bacteria in the VBNC state do not form colonies, and thus, are difficult to detect using colony-based methods. As a result, VBNC bacteria are potentially virulent and can cause widespread contamination during food production. In the present study, we reported a novel biomarker, the membrane vesicle protein PagC, for the detection of VBNC Salmonella. Salmonella cells were chemically induced into the VBNC state by H2O2 treatment...
December 4, 2017: Journal of Veterinary Medical Science
G Mandilara, C M Vassalos, A Chrisostomou, K Karadimas, E Mathioudaki, T Georgakopoulou, S Tsiodras, K Mellou
In June 2016, a Salmonella enterica serovar Enteritidis outbreak (n = 56) occurred after a christening reception in Central Greece, mainly affecting previously healthy adults; one related death caused media attention. Patients suffered from profuse diarrhoea, fever and frequent vomiting episodes requiring prolonged hospitalisation and sick leave from work, with a 54% hospital admission rate. The majority of cases experienced serious illness within <12 h of attending the party. We investigated the outbreak to identify the source(s) of infection and contributing factors to the disease severity...
December 4, 2017: Epidemiology and Infection
Tamara Calvo, Avelino Alvarez-Ordóñez, Miguel Prieto, Ana Bernardo, Mercedes López
This study was aimed at studying the influence of gas composition (air and nitrogen) at different flow rates (5, 10 and 15Lm-1) and stress adaptation (growth under a wide range of temperatures [10-45°C] and acid conditions [up to pH4.5, using different organic acids] or short-term exposure to acid, cold or heat stress shocks) on the inactivation by Non-Thermal Atmospheric Plasma (NTAP) of S. Typhimurium CECT 443 and S. Enteritidis CECT 4300. Results obtained evidence that microbial inactivation was significantly higher when air was used for NTAP treatments...
December 2017: Food Research International
Karen Fong, Brett LaBossiere, Andrea I M Switt, Pascal Delaquis, Lawrence Goodridge, Roger C Levesque, Michelle D Danyluk, Siyun Wang
Alfalfa sprouts have been linked to numerous North American outbreaks of Salmonella in recent years. Conventionally, treatments involving chlorine, heat, and irradiation are used for alfalfa seed sanitation. However, such treatments may be highly variable in their efficacy for pathogen control and/or detrimental to sprout quality, therefore negatively perceived by consumers advocating for natural alternatives. The usage of bacteriophages for pathogen control in sprouts has been previously explored, although with conflicting and inconsistent results...
2017: Frontiers in Microbiology
Arnaud Felten, Meryl Vila Nova, Kevin Durimel, Laurent Guillier, Michel-Yves Mistou, Nicolas Radomski
BACKGROUND: Many of the bacterial genomic studies exploring evolution processes of the host adaptation focus on the accessory genome describing how the gains and losses of genes can explain the colonization of new habitats. Consequently, we developed a new approach focusing on the coregenome in order to describe the host adaptation of Salmonella serovars. METHODS: In the present work, we propose bioinformatic tools allowing (i) robust phylogenetic inference based on SNPs and recombination events, (ii) identification of fixed SNPs and InDels distinguishing homoplastic and non-homoplastic coregenome variants, and (iii) gene-ontology enrichment analyses to describe metabolic processes involved in adaptation of Salmonella enterica subsp...
November 28, 2017: BMC Microbiology
Rebecca-Ayme Hughes, Riawana A Ali, Mary A Mendoza, Hosni M Hassan, Matthew D Koci
Preventing Salmonella colonization in young birds is key to reducing contamination of poultry products for human consumption (eggs and meat). While several Salmonella vaccines have been developed that are capable of yielding high systemic antibodies, it is not clear how effective these approaches are at controlling or preventing Salmonella colonization of the intestinal tract. Effective alternative control strategies are needed to help supplement the bird's ability to prevent Salmonella colonization, specifically by making the cecum less hospitable to Salmonella...
2017: Frontiers in Veterinary Science
Sabina Horvat, Aleksander Mahnic, Martin Breskvar, Saso Dzeroski, Maja Rupnik
Clostridium difficile infection (CDI) is typically associated with disturbed gut microbiota and changes related to decreased colonization resistance against C. difficile are well described. However, nothing is known about possible effects of C. difficile on gut microbiota restoration during or after CDI. In this study, we have mimicked such a situation by using C. difficile conditioned medium of six different C. difficile strains belonging to PCR ribotypes 027 and 014/020 for cultivation of fecal microbiota...
November 27, 2017: Scientific Reports
Dora Hancz, Aniko Szabo, Tamás Molnar, Zsofia Varga, Aniko Hancz, Andrea Gregus, Anne-Odile Hueber, Eva Rajnavolgyi, Gabor Koncz
Efficient adjuvants have the potential to trigger both innate and adaptive immune responses simultaneously. Flagellin is a unique pathogen-derived protein, which is recognized by pattern recognition receptors (PRRs) as well as by B-cell and T cell receptors thus providing an important link between innate and adaptive immunity. The aforementioned properties define flagellin as an optimal adjuvant. The induction of immunogenic cell death could be an additional expectation for adjuvants in the context of cancer immunotherapy due to their ability to activate dendritic cells (DC) to present tumor antigens through the engulfment of dying cells...
November 22, 2017: Immunology Letters
Anthoula A Argyri, Olga S Papadopoulou, Aspasia Nisiotou, Chrysoula C Tassou, Nikos Chorianopoulos
High pressure processing (HPP) is a preservation technology alternative to heat treatment that is mild for food, but effectively inactivates the spoilage microbiota and foodborne pathogens of several foods. The purpose of the current study was to evaluate the effect of HPP on Salmonella ser. Enteritidis, indigenous microbiota and shelf-life of chicken fillets. Chicken fillets were inoculated with S. Enteritidis at three different initial inocula (3, 5, 7 log CFU/g), packed under vacuum, treated or not with HPP (500 MPa/10 min) and stored at 4 and 12 °C...
April 2018: Food Microbiology
Kurt E Richardson, Nelson A Cox, Douglas E Cosby, Mark E Berrang
In a recent study, the pH of commonly used Salmonella pre-enrichment media became acidic (pH 4.0 to 5.0) when feed or feed ingredients were incubated for 24 h. Acidic conditions have been reported to injure or kill Salmonella. In this study, cultures of four known feed isolates (S. montevideo, S. senftenberg, S. tennessee, and S. schwarzengrund) and four important processing plant isolates (S. typhimurium, S. enteritidis, S. infantis, and S. heidelberg) were grown on meat and bone meal and later subjected to desiccation and heat exposure to stress the microorganism...
November 27, 2017: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
Patricia Barrios, Federica Badía, Valentina Misa, M Inés Mota, Arací Martínez, Hebert Mariño, Gabriela Algorta, Javier Prego, M Catalina Pírez
BACKGROUND: Salmonella can cause asymptomatic infections, diarrhea, bacteremia and focal infections such as meningitis and osteomyelitis. AIM: To describe clinical and microbiological aspects of infections by Salmonella spp. in children in a pediatric referral hospital: Centro Hospitalario Pereira Rossell, in Montevideo, Uruguay. MATERIALS AND METHODS: Descriptive and retrospective study of 46 patients, from which Salmonella spp was isolated between January 1, 2005 and December 31, 2010...
August 2017: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
Spyridon Petropoulos, Ângela Fernandes, Lillian Barros, Ana Ciric, Marina Sokovic, Isabel C F R Ferreira
Okra is a vegetable crop usually used for its immature pods. The harvest stage (fruit size) depends on consumers' preferences and the fruit that does not meet market requirements is being disposed of. Considering the short time interval from the setting of the fruit to the harvest stage, the present study evaluates the nutritional value, chemical composition, and antioxidant and antimicrobial properties of okra seeds from genotypes cultivated under Mediterranean conditions, as an alternative end-use product...
November 22, 2017: Food & Function
Song Li, Yufa Zhou, Zengmin Miao
The present study was aimed to determine the prevalence and characteristics of Salmonella isolated from meat samples of commercial broilers (CB) and spent hens (SH). Between March and June 2016, 200 retail raw chicken carcasses (100 from CB and 100 from SH) were obtained from local supermarkets in Tai'an city of China, and Salmonella isolates were then analyzed for antibiotic resistance, serotype, β-lactamase genes, and the presence of class 1 integron. Forty Salmonella strains were obtained in this study (CB: 21/100, 21%; SH: 19/100, 19%)...
2017: Frontiers in Microbiology
In Young Choi, Ju-Hoon Lee, Hye-Jin Kim, Mi-Kyung Park
Bacteriophages have gained substantial attention as biocontrol and bio-recognition agents, substituting antibodies. In this study, a Salmonella Enteritidis-specific bacteriophage, KFS-SE1, was isolated, identified, and characterized. This Siphoviridae phage infects S. Enteritidis with high specificity. This phage is highly stable under various pH (5-11), temperature (4-60°C), and organic solvent conditions. The KFS-SE1 genome consists of 59,715-bp with 73 predicted open reading frames and 57.14% GC content; it has a complete set of genes required for phage reconstruction...
November 21, 2017: Journal of Microbiology and Biotechnology
Mayada Mahmoud, Ahmed Askora, Ahmed Barakat Barakat, Omar El-Farouk Rabie, Sayed Emam Hassan
In this study, we isolated and characterized three phages named as Salmacey1, Salmacey2 and Salmacey3, infecting multi drug resistant Salmonella serovars isolated from broilers in Egypt. The most prevalent Salmonella serovars were S. typhimurium, S. enteritidis, and S. kentucky. All these Salmonella serovars were found to be resistant to more than two of the ten antimicrobial agents tested. Only S. kentucky was found to be resistant to seven antimicrobial agents. Examination of these phage particles by transmission electron microscopy (TEM), demonstrated that two phages (Salmacey1, Salmacey2) were found to belong to family Siphoviridae, and Salmacey3 was assigned to the family Myoviridae...
November 14, 2017: International Journal of Food Microbiology
Abhishek K Singh, Suchita Prasad, Bipul Kumar, Shiv Kumar, Amitesh Anand, Shashank S Kamble, Sunil K Sharma, Hemant K Gautam
Four (1, 2, 4 and 6) synthetic quaternary ammonium derivatives of pyranochromenones and (coumarinyloxy)acetamides were synthesized and investigated for their antimicrobial efficacy on MRSA (Methicillin-resistant Staphylococcus aureus), and multi-drug resistant Pseudomonas aeruginosa, Salmonella enteritidis and Mycobacterium tuberculosis H37Rv strain. One of the four compounds screened i.e. N,N,N-triethyl-10-((4,8,8-trimethyl-2-oxo-2,6,7,8-tetrahydropyrano[3,2-g]chromen-10-yl)oxy)decan-1-aminium bromide (1), demonstrated significant activity against S...
December 2017: Indian Journal of Microbiology
Alexandre Lamas, José Manuel Miranda, Patricia Regal, Beatriz Vázquez, Carlos Manuel Franco, Alberto Cepeda
Salmonella is a major foodborne pathogen with a complex nomenclature. This genus is composed of two species, S. enterica and S. bongori. S. enterica is divided into six subspecies. S. enterica subspecies enterica is composed of more than 1500 serotypes with some of great importance, such as S. Typhimurium and S. Enteritidis. S. enterica subsp. enterica is responsible of more than 99% of human salmonellosis and therefore it is widely studied. However, the non-enterica subspecies of S. enterica have been little studied...
January 2018: Microbiological Research
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