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https://www.readbyqxmd.com/read/28102821/salmonella-enteritidis-mycotic-aneurysm-of-stented-saphenous-vein-graft-to-coronary-artery
#1
Arka Chatterjee, Jeremy S White, Benjamin Tuck, James E Davies
No abstract text is available yet for this article.
November 28, 2016: JACC. Cardiovascular Interventions
https://www.readbyqxmd.com/read/28096617/phenotypic-and-genotypic-characterization-of-locally-isolated-salmonella-strains-used-in-preparation-of-salmonella-antigens-in-egypt
#2
Hazem Mohammed Ibrahim, Dalia Ahmed Mohammed Abd El-Moaty, Hanan Ali Ahmed, Mona Ibrahim El-Enbaawy
AIM: This work was conducted to study the phenotypic and genotypic characterization of locally isolated Salmonella strains (Salmonella Pullorum, Salmonella Enteritidis, and Salmonella Typhimurium) from poultry used in the preparation of Salmonella antigens in Egypt. MATERIALS AND METHODS: The phenotypic characterization of Salmonella strains was done using standard microbiological, biochemical, and serological techniques. Molecular identification was done using different sets of primers on different genes using different polymerase chain reaction (PCR) techniques...
December 2016: Veterinary World
https://www.readbyqxmd.com/read/28094807/antimicrobial-probiotics-reduce-salmonella-enterica-in-turkey-gastrointestinal-tracts
#3
Brittany Forkus, Seth Ritter, Michail Vlysidis, Kathryn Geldart, Yiannis N Kaznessis
Despite the arsenal of technologies employed to control foodborne nontyphoidal Salmonella (NTS), infections have not declined in decades. Poultry is the primary source of NTS outbreaks, as well as the fastest growing meat sector worldwide. With recent FDA rules for phasing-out antibiotics in animal production, pressure is mounting to develop new pathogen reduction strategies. We report on a technology to reduce Salmonella enteritidis in poultry. We engineered probiotic E. coli Nissle 1917, to express and secrete the antimicrobial peptide, Microcin J25...
January 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28086873/cecal-micrornaome-response-to-salmonella-enterica-serovar-enteritidis-infection-in-white-leghorn-layer
#4
Guixian Wu, Yukai Qi, Xiaoyi Liu, Ning Yang, Guiyun Xu, Liying Liu, Xianyao Li
BACKGROUND: Salmonella enterica serovar Enteritidis (SE) is a food-borne pathogen and of great threat to human health through consuming the contaminated poultry products. MicroRNAs (miRNAs) play an important role in different biological activities and have been shown to regulate the innate immunity in the bacterial infection. The objective of this study is to identify miRNAs associated with SE infection in laying chicken cecum. RESULTS: Average number of reads of three libraries constructed from infected and non-infected chickens was 12,476,156 and 10,866,976, respectively...
January 13, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28078872/molecular-characterization-and-antibiotic-resistance-of-salmonella-serovars-isolated-in-the-apulia-region-of-italy
#5
R Mirgaldi, A Ballini, A M Dionisi, I Luzzi, G Dipalma, F Inchingolo, D De Vito
During the period January 2013-December 2015, 175 cases of human salmonellosis were reported in the Apulia Region of Italy. The aim of this study was to characterize salmonella strains from the standpoints of serovars prevalence, antimicrobial resistance and clonal origin. The serological typing was performed by agglutination against antisera followed by a multiplex polymerase chain reaction (m-PCR). The obtained results were analyzed following the Kauffmann-White scheme. Susceptibility to antimicrobial agents was tested using the disk diffusion method on Muller-Hinton agar plates...
October 2016: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/28074119/complete-genome-sequence-of-the-salmonella-enterica-serovar-enteritidis-bacteriophages-fse1c-and-fse4c-isolated-from-food-matrices
#6
Javier Santander, Jose I Vasquez, Cristopher Segovia, Leonardo Santos, Gabriel Turra, Karen Huber, James Robeson
Salmonella enterica serovar Enteritidis is one of the most common causes of Salmonellosis worldwide. Utilization of bacteriophages as prophylactic agents is a practical solution to prevent Salmonellosis in ready-to-eat products. Shelf stability is one of the desirable properties for prophylactic bacteriophages. Here, we describe the phenotype, genome, and phylogeny of fSE1C and fSE4S Salmonella bacteriophages. fSE1C and fSE4S were previously isolated from pickle sauce and ground beef respectively and selected for their significant shelf stability...
2017: Standards in Genomic Sciences
https://www.readbyqxmd.com/read/28063730/transmission-of-salmonella-enteritidis-after-endoscopic-retrograde-cholangiopancreatography-because-of-inadequate-endoscope-decontamination
#7
Paul Robertson, Andrew Smith, Margaret Anderson, Jackie Stewart, Kate Hamilton, Sandra McNamee, Evonne T Curran
We report a historic nosocomial outbreak of Salmonella enteritidis affecting 4 inpatients who underwent endoscopic retrograde cholangiopancreatography. The cause was attributed to inadequate decontamination of an on-loan endoscope used over a weekend. This report highlights the risks of using on-loan endoscopes, particularly regarding their commissioning and adherence to disinfection protocols. In an era of increasing antibiotic resistance, transmission of Enterobacteriaceae by endoscopes remains a significant concern...
January 4, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/28063704/immunization-with-salmonella-enteritidis-secreting-mucosal-adjuvant-labile-toxin-confers-protection-against-wild-type-challenge-via-augmentation-of-cd3-cd4-t-cell-proliferation-and-enhancement-of-ifn-%C3%AE-il-6-and-il-10-expressions-in-chicken
#8
Jonathan Lalsiamthara, John Hwa Lee
The protective efficacy and immunological profiles of chickens immunized with an attenuated Salmonella Enteritidis (SE) constitutively secreting double mutant heat labile enterotoxin (dmLT) were investigated. The dmLT is a detoxified variant of Escherichia coli heat labile toxin and is a potent mucosal adjuvant capable of inducing both humoral and cell-mediated immunity. In this study, four-week-old chickens were inoculated with SE-dmLT strain JOL1641, parental SE strain JOL1087 or phosphate buffered saline control...
January 4, 2017: Vaccine
https://www.readbyqxmd.com/read/28056035/a-systematic-review-of-the-incidence-risk-factors-and-case-fatality-rates-of-invasive-nontyphoidal-salmonella-ints-disease-in-africa-1966-to-2014
#9
Ifeanyi Valentine Uche, Calman A MacLennan, Allan Saul
This study systematically reviews the literature on the occurrence, incidence and case fatality rate (CFR) of invasive nontyphoidal Salmonella (iNTS) disease in Africa from 1966 to 2014. Data on the burden of iNTS disease in Africa are sparse and generally have not been aggregated, making it difficult to describe the epidemiology that is needed to inform the development and implementation of effective prevention and control policies. This study involved a comprehensive search of PubMed and Embase databases...
January 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28051328/a-single-step-enrichment-medium-for-nonchromogenic-isolation-of-healthy-and-cold-injured-salmonella-spp-from-fresh-vegetables
#10
Hong-Seok Kim, Dasom Choi, Il-Byeong Kang, Dong-Hyeon Kim, Jin-Hyeok Yim, Young-Ji Kim, Jung-Whan Chon, Deog-Hwan Oh, Kun-Ho Seo
Culture-based detection of nontyphoidal Salmonella spp. in foods requires at least four working days; therefore, new detection methods that shorten the test time are needed. In this study, we developed a novel single-step Salmonella enrichment broth, SSE-1, and compared its detection capability with that of commercial single-step ONE broth-Salmonella (OBS) medium and a conventional two-step enrichment method using buffered peptone water and Rappaport-Vassiliadis soy broth (BPW-RVS). Minimally processed lettuce samples were artificially inoculated with low levels of healthy and cold-injured Salmonella Enteritidis (10(0) or 10(1) colony-forming unit/25 g), incubated in OBS, BPW-RVS, and SSE-1 broths, and streaked on xylose lysine deoxycholate (XLD) agar...
January 4, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28049014/chitosan-sulfated-locust-bean-gum-nanoparticles-in-vitro-and-in-vivo-evaluation-towards-an-application-in-oral-immunization
#11
Luis Braz, Ana Grenha, Domingos Ferreira, Ana M Rosa da Costa, Carlos Gamazo, Bruno Sarmento
This work proposes the design of nanoparticles based on locus bean gum (LBG) and chitosan to be used as oral immunoadjuvant for vaccination purposes. LBG-based nanoparticles were prepared by mild polyelectrolyte complexation between chitosan (CS) and a synthesized LBG sulfate derivative (LBGS). Morphological characterization suggested that nanoparticles present a solid and compact structure with spherical-like shape. Sizes around 180-200nm and a positive surface charge between +9mV and +14mV were obtained. CS/LBGS nanoparticles did not affect cell viability of Caco-2 cells after 3h and 24h of exposure when tested at concentrations up to 1...
December 31, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28040162/occurrence-and-characterization-of-food-borne-pathogens-isolated-from-fruit-vegetables-and-sprouts-retailed-in-the-czech-republic
#12
Hana Vojkovská, Petra Myšková, Tereza Gelbíčová, Alena Skočková, Ivana Koláčková, Renáta Karpíšková
Food of non-animal origin is a major component of the human diet and has been considered to pose a low risk from the point of view of bacteriological safety. However, an increase in the number of outbreaks of illness caused by such pathogens and linked to the consumption of fresh fruit and vegetables have been reported from around the world recently. Salmonella spp., STEC (Shiga toxin producing Escherichia coli) and Listeria monocytogenes are among the most frequently identified agents. Additionally, the transmission of antibiotic resistant strains including also the methicillin resistant S...
May 2017: Food Microbiology
https://www.readbyqxmd.com/read/28018331/construction-of-a-pan-genome-allele-database-of-salmonella-enterica-serovar-enteritidis-for-molecular-subtyping-and-disease-cluster-identification
#13
Yen-Yi Liu, Chih-Chieh Chen, Chien-Shun Chiou
We built a pan-genome allele database with 395 genomes of Salmonella enterica serovar Enteritidis and developed computer tools for analysis of whole genome sequencing (WGS) data of bacterial isolates for disease cluster identification. A web server (http://wgmlst.imst.nsysu.edu.tw) was set up with the database and the tools, allowing users to upload WGS data to generate whole genome multilocus sequence typing (wgMLST) profiles and to perform cluster analysis of wgMLST profiles. The usefulness of the database in disease cluster identification was demonstrated by analyzing a panel of genomes from 55 epidemiologically well-defined S...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28003197/elucidation-of-antibacterial-mechanism-of-405%C3%A2-5-nm-light-emitting-diode-against-salmonella-spp-at-refrigeration-temperature
#14
Min-Jeong Kim, Hyun-Gyun Yuk
: The aim of this study was to elucidate the antibacterial mechanism of 405±5 nm light-emitting diode (LED) illumination against Salmonella at 4 °C in phosphate-buffered saline (PBS) by determining endogenous coproporphyrin content, DNA oxidation, damage to membrane function, and morphological change. Gene expression levels, including oxyR, recA, rpoS, sodA, and soxR, were also examined to understand the response of Salmonella to LED illumination. Results showed that Salmonella spp...
December 21, 2016: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27999166/the-cytolethal-distending-toxin-produced-by-nontyphoidal-salmonella-serotypes-javiana-montevideo-oranienburg-and-mississippi-induces-dna-damage-in-a-manner-similar-to-that-of-serotype-typhi
#15
Rachel A Miller, Martin Wiedmann
: Select nontyphoidal Salmonella enterica (NTS) serotypes were recently found to encode the Salmonella cytolethal distending toxin (S-CDT), an important virulence factor for serotype Typhi, the causative agent of typhoid fever. Using a PCR-based assay, we determined that among 21 NTS serotypes causing the majority of food-borne salmonellosis cases in the United States, genes encoding S-CDT are conserved in isolates representing serotypes Javiana, Montevideo, and Oranienburg but that among serotype Mississippi isolates, the presence of S-CDT-encoding genes is clade associated...
December 20, 2016: MBio
https://www.readbyqxmd.com/read/27998639/characterization-of-o-antigen-delivered-by-generalized-modules-for-membrane-antigens-gmma-vaccine-candidates-against-nontyphoidal-salmonella
#16
G De Benedetto, R Alfini, P Cescutti, M Caboni, L Lanzilao, F Necchi, A Saul, C A MacLennan, S Rondini, F Micoli
Invasive nontyphoidal Salmonella disease (iNTS) is a leading cause of death and morbidity in Africa. The most common pathogens are Salmonella enterica serovars Typhimurium and Enteritidis. The O-antigen portion of their lipopolysaccharide is a target of protective immunity and vaccines targeting O-antigen are currently in development. Here we investigate the use of Generalized Modules for Membrane Antigens (GMMA) as delivery system for S. Typhimurium and S. Enteritidis O-antigen. Gram-negative bacteria naturally shed outer membrane in a blebbing process...
January 11, 2017: Vaccine
https://www.readbyqxmd.com/read/27998412/-molecular-typing-of-salmonella-isolates-from-poultry-production-chains-in-four-cities-of-heilongjiang-province
#17
X J Yu, X Liang, L Bai, W W Li, J Yan, K L Wang, X Li
Objective: To study the PFGE type of Salmonella (S.) strains isolated from poultry production chains (hatching, breeding, slaughter, distribution and retail) of four cities in Heilongjiang province. Methods: DNA collected from S. strains in 2012 was digested by XbaⅠ according to the standard PFGE protocol of US CDC. The PFGE patterns were then analyzed by BioNumerics software. Results: The contamination of S. appeared most serious during the process of slaughtering (13.84%). PFGE was used to determine the genetic relationships between these isolates from poultry production chains, 89 pulsotypes from 150 S...
December 10, 2016: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/27993596/molecular-detection-assay-of-five-salmonella-serotypes-of-public-interest-typhimurium-enteritidis-newport-heidelberg-and-hadar
#18
M Bugarel, A Tudor, G H Loneragan, K K Nightingale
Foodborne illnesses due to Salmonella represent an important public-health concern worldwide. In the United States, a majority of Salmonella infections are associated with a small number of serotypes. Furthermore, some serotypes that are overrepresented among human disease are also associated with multi-drug resistance phenotypes. Rapid detection of serotypes of public-health concern might help reduce the burden of salmonellosis cases and limit exposure to multi-drug resistant Salmonella. We developed a two-step real-time PCR-based rapid method for the identification and detection of five Salmonella serotypes that are either overrepresented in human disease or frequently associated with multi-drug resistance, including serotypes Enteritidis, Typhimurium, Newport, Hadar, and Heidelberg...
December 18, 2016: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/27987122/effective-characterization-of-salmonella-enteritidis-by-most-probable-number-mpn-followed-by-multiplex-polymerase-chain-reaction-pcr-methods
#19
Lincohn Zappelini, Solange Martone-Rocha, Milena Dropa, Maria Helena Matté, Monique Ribeiro Tiba, Bruna Suellen Breternitz, Maria Tereza Pepe Razzolini
Nontyphoidal Salmonella (NTS) is a relevant pathogen involved in gastroenteritis outbreaks worldwide. In this study, we determined the capacity to combine the most probable number (MPN) and multiplex polymerase chain reaction (PCR) methods to characterize the most important Salmonella serotypes in raw sewage. A total of 499 isolates were recovered from 27 raw sewage samples and screened using two previously described multiplex PCR methods. From those, 123 isolates were selected based on PCR banding pattern-identical or similar to Salmonella Enteritidis and Salmonella Typhimurium-and submitted to conventional serotyping...
December 16, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27965649/the-12-gastrointestinal-pathogens-spectrum-of-acute-infectious-diarrhea-in-a-sentinel-hospital-shenzhen-china
#20
Hongwei Shen, Jinjin Zhang, Yinghui Li, Sirou Xie, Yixiang Jiang, Yanjie Wu, Yuhui Ye, Hong Yang, Haolian Mo, Chaoman Situ, Qinghua Hu
Acute infectious gastroenteritis is one of the most common diseases among all ages, particularly in developing countries. The pathogen spectrum may differ among different regions and seasons. To investigate the etiology of acute diarrhea in Shenzhen, a prospective study was conducted from August 2014 to September 2015. Stools from 412 patients with diarrhea (286 of whom were adults) including the general epidemiological information of the patients were collected. The 19 pathogens were detected by conventional culture method or multiplex PCR assay, which included five viruses (rotavirus, adenovirus, sapovirus, norovirus, and astrovirus), 11 bacterial pathogens (Salmonella, Campylobacter jejuni, Shigella, Listeria monocytogenes, Vibrio parahaemolyticus, Vibrio cholera, Enterohemorrhagic (EHEC), enteropathogenic (EPEC), enteroinvasive (EIEC), enterotoxigenic (ETEC); and enteroaggregative Escherichia coli (EAEC)) and three parasites (Entamoeba histolytica, Giardia lamblia, and Cryptosporidium parvum)...
2016: Frontiers in Microbiology
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