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https://www.readbyqxmd.com/read/28543032/influence-of-different-wild-garlic-allium-ursinum-extracts-on-the-gastrointestinal-system-spasmolytic-antimicrobial-and-antioxidant-properties
#1
Dragana R Pavlović, Milica Veljković, Nikola M Stojanović, Marija Gočmanac-Ignjatović, Tatjana Mihailov-Krstev, Suzana Branković, Dušan Sokolović, Mirjana Marčetić, Niko Radulović, Mirjana Radenković
OBJECTIVES: As there are no previous studies of the European wild-garlic (Allium ursinum) effects on the gastrointestinal system, despite its traditional applications in gastrointestinal disorders' treatment and regular use in the human diet, we have quantified and compared spasmolytic, antimicrobial and antioxidant activities of its different leaf extracts. METHODS: Wild-garlic extracts were tested for spasmolytic activity on isolated rat ileum, antimicrobial activity on selected Gram-positive and Gram-negative bacteria and fungi by microdilution method and antioxidant capacity by DPPH radical-scavenging assay...
May 23, 2017: Journal of Pharmacy and Pharmacology
https://www.readbyqxmd.com/read/28542593/lack-of-the-pga-exopolysaccharide-in-salmonella-as-an-adaptive-trait-for-survival-in-the-host
#2
Maite Echeverz, Begoña García, Amaia Sabalza, Jaione Valle, Toni Gabaldón, Cristina Solano, Iñigo Lasa
Many bacteria build biofilm matrices using a conserved exopolysaccharide named PGA or PNAG (poly-β-1,6-N-acetyl-D-glucosamine). Interestingly, while E. coli and other members of the family Enterobacteriaceae encode the pgaABCD operon responsible for PGA synthesis, Salmonella lacks it. The evolutionary force driving this difference remains to be determined. Here, we report that Salmonella lost the pgaABCD operon after the divergence of Salmonella and Citrobacter clades, and previous to the diversification of the currently sequenced Salmonella strains...
May 24, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28539041/outer-membrane-vesicles-derived-from-salmonella-enteritidis-protect-against-the-virulent-wild-type-strain-infection-in-a-mouse-model
#3
Qiong Liu, Jie Yi, Kang Liang, Xiangmin Zhang, Qing Liu
Food-borne contamination and salmonellosis caused by Salmonella Enteritidis (S. Enteritidis) are a significant threat to human health and poultry enterprises. Outer membrane vesicles (OMVs), which are naturally secreted by gram-negative bacteria, could be a good vaccine option because they have many biologically active substances, including lipopolysaccharides (LPS), outer membrane proteins (OMPs), and phospholipids, as well as periplasmic components. In the present study, we purified OMVs derived from S. Enteritidis and analyzed their characteristics through silver staining and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)...
May 25, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28533247/first-detection-of-a-fosfomycin-resistance-gene-fosa7-in-salmonella-enterica-serovar-heidelberg-isolated-from-broiler-chickens
#4
Muhammad A Rehman, Xianhua Yin, Marissa G Persaud-Lachhman, Moussa S Diarra
We previously described Salmonella Heidelberg harbouring a chromosomal gene cluster similar to the glutathione S-transferase gene, a putative fosA conferring resistance to fosfomycin. Here we show that this new gene named fosA7 confers resistance to fosfomycin. Introduction of fosA7 into fosfomycin susceptible Salmonella Enteritidis, resulted in a substantial increase in the fosfomycin MIC. This finding increases awareness of antibiotic resistance in Salmonella Heidelberg from broiler related to the food safety and public health...
May 22, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28532797/genetic-basis-for-loss-of-immuno-reactive-o-chain-in-salmonella-enterica-serovar-enteritidis-veterinary-isolates
#5
Istvan Szabo, Marianne Grafe, Nicole Kemper, Ernst Junker, Burkhard Malorny
Fifty-two rough Salmonella enterica serovar Enteritidis (S. Enteritidis) isolates from broilers and the environment were characterized for their serological and genotypic properties. Under routine diagnostic serotyping methods such isolates lack the immuno-reactivity of the O-chain of the lipopolysaccharide (LPS), and are referred to as non-typeable. Using a modified slide agglutination method, the isolates could be differentiated into three different serological variants. Twenty-six isolates (50%) were defined as semi-rough, nineteen isolates (37%) as deep-rough, four isolates (8%) as rough and three isolates could not be assigned...
May 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28513808/magnetic-nanoparticle-enhanced-pcr-for-the-detection-and-identification-of-staphylococcus-aureus-and-salmonella-enteritidis
#6
Dimitra Houhoula, Joseph Papaparaskevas, Katerina Zatsou, Nikolaos Nikolaras, Hanan I Malkawi, Marie-Paule Mingenot-Leclercq, Spyros Konteles, Stamatis Koussisis, Athanassios Tsakris, Ekatherina Charvalos
This paper evaluated magnetic nanoparticle-enhanced PCR for the detection and identification of Staphylococcus aureus and Salmonella enteritidis. Two different types of magnetic nanoparticles designated MPIO (iron concentration 2.5 mg/ml, size 1 μm) and NP (iron concentration 8.7 mg/ml, size 60 nm), both conjugated with S. aureus or S. enteritidis antibodies were evaluated as an enrichment procedure for PCR-detection of the pathogens in Trypticase Soy Broth, milk, blood and meat broth. Bacterial suspensions (1...
May 17, 2017: New Microbiologica
https://www.readbyqxmd.com/read/28513778/phenotypic-and-genotypic-characterization-of-salmonella-spp-isolated-from-foods-and-clinical-samples-in-brazil
#7
Andrea L Miranda, Soraia M Cordeiro, Joice N Reis, Lucas G Cardoso, Alaíse G Guimarães
Increasing antimicrobial resistance in Salmonella species has been a serious problem for public health worldwide. This study examines Salmonella spp. recovered from foods and clinical samples on serotype, antimicrobial resistance and PFGE genotypes. It identified 91 salmonellae, belonging to 31 different serotypes, from 36 isolates from food and 55 clinical samples. Salmonella Infantis (16.5%) and Salmonella Enteritidis (13.7%) are the most common among food isolates, whereas Salmonella Enteritidis (29.0%) and Salmonella Typhimurium (16...
May 15, 2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/28502116/phenolic-composition-anti-inflammatory-antioxidant-and-antimicrobial-activities-of-alchemilla-mollis-buser-rothm
#8
Gökçe Şeker Karatoprak, Selen İlgün, Müberra Koşar
The current study was designed to evaluate the antioxidant, anti-inflammatory and antimicrobial activities of Alchemilla mollis (Buser) Rothm. (Rosaceae) aerial parts extracts. Chemical composition was analyzed by spectrophotometric and chromatographic (HPLC) techniques. The antioxidant properties assessed included, DPPH(●) and ABTS(●+) radical scavenging, β-carotene-linoleic acid co-oxidation assay. Antimicrobial activity was evaluated with disc diffusion and micro dilution method. In order to evaluate toxicity of the extracts with the sulforhodamine B (SRB) colorimetric assay L929 cell line (mouse fibroblast) was used...
May 14, 2017: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/28500041/evaluation-of-a-real-time-pcr-method-for-detection-of-salmonella-spp-in-environmental-samples
#9
Kuppuswamy N Kasturi, Tomas Drgon
Methods currently used for detecting Salmonella in environmental samples require two days to produce results and have limited sensitivity. Here, we describe the development and validation of a real-time PCR Salmonella screening method that produces results in 18 to 24 h. Primers and probes specific to the gene invA, group D, and Enteritidis serovar were designed and evaluated for inclusivity and exclusivity using a panel of 329 Salmonella isolates representing 126 serovars and 22 non-Salmonella organisms. The invA and group D specific sets identified all the isolates accurately...
May 12, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28495316/effect-of-infectious-bursal-disease-ibd-vaccine-on-salmonella-enteritidis-infected-chickens
#10
Nagah Arafat, Abdelfattah H Eladl, Hebatallah Mahgoub, Reham A El-Shafei
BACKGROUND: Chickens infected with both infectious bursal disease virus (IBDV) and Salmonella had higher mortality. In this work, we investigated the effect of IBDV vaccine (modified live-virus bursal disease vaccine, Nobilis strain 228E®) on experimentally infected chickens with Salmonella Enteritidis (SE). METHODS: Four experimental groups were included in this study, negative control group, 228E®group, 228E®+SE infected group, and SE infected group. Chickens were ocularly administrated 228E® at 12days of age and orally infected with S...
May 8, 2017: Vaccine
https://www.readbyqxmd.com/read/28488775/effects-of-sorbic-acid-chitosan-microcapsules-as-antimicrobial-agent-on-the-properties-of-ethylene-vinyl-alcohol-copolymer-film-for-food-packaging
#11
Shuaifeng Hu, Jie Yu, Zhe Wang, Li Li, Yunfei Du, Liping Wang, Yuan Liu
This paper discusses the possibility of using sorbic acid-chitosan microcapsules (S-MPs) as an antibacterial component of active ethylene vinyl alcohol copolymer (EVOH) film. S-MPs with a diameter of approximately 1 to 4 μm showed a sorbic acid loading capacity of 46.5%. Addition of S-MPs (3%, w/w) increased the tensile strength, haze, oxygen, and water vapor barrier properties, as well as reduced the elongations at break and transmittance of S-MPs-EVOH (S-MP-EVOH) film. Antibacterial tests showed that the inhibitory capacity of S-MP-EVOH film against Salmonella Enteritidis and Escherichia coli was higher than that against Listeria monocytogenes...
May 10, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28475359/assessment-of-consumer-exposure-to-salmonella-spp-campylobacter-spp-and-shiga-toxin-producing-escherichia-coli-in-meat-products-at-retail-in-the-city-of-sao-paulo-brazil
#12
Christiane Asturiano Ristori, Ruth Estela Gravato Rowlands, Cecília Geraldes Martins, Maria Luisa Barbosa, Luis Fernando Dos Santos, Miyoko Jakabi, Bernadette Dora Gombossy de Melo Franco
Meat products may be vehicles of bacterial pathogens to humans, and Salmonella spp., Campylobacter spp., and Shiga toxin-producing Escherichia coli (STEC) are the most relevant. The aim of this study was to generate data on prevalence of these three pathogens in 552 samples of meat products (hot dogs, pork sausages, raw ground beef, and raw chicken legs) sold at retail in the city of Sao Paulo, Brazil. Salmonella spp. was detected in 5.8% (32/552) of samples, comprising pork sausages 62.5% (20/32) and chicken legs 37...
May 5, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28463976/urine-disinfection-and-in-situ-pathogen-killing-using-a-microbial-fuel-cell-cascade-system
#13
Ioannis Ieropoulos, Grzegorz Pasternak, John Greenman
Microbial Fuel Cells (MFCs) are emerging as an effective means of treating different types of waste including urine and wastewater. However, the fate of pathogens in an MFC-based system remains unknown, and in this study we investigated the effect of introducing the enteric pathogen Salmonella enterica serovar enteritidis in an MFC cascade system. The MFCs continuously fed with urine showed high disinfecting potential. As part of two independent trials, during which the bioluminescent S. enteritidis strain was introduced into the MFC cascade, the number of viable counts and the level of bioluminescence were reduced by up to 4...
2017: PloS One
https://www.readbyqxmd.com/read/28459222/isolation-and-characterization-of-salmonella-enteritidis-and-salmonella-typhimurium-from-chicken-meat-in-egypt
#14
Reda Tarabees, Mohamed S A Elsayed, Reyad Shawish, Shereen Basiouni, Awad A Shehata
INTRODUCTION: Salmonella enterica serovars Enteritidis and Typhimurium represent the major serovars associated with human salmonellosis. Contamination of meat products with these serovars is considered the main source of infection. METHODOLOGY: In this study, 100 raw chicken meat samples were investigated for the presence of Salmonella spp., which were subsequently identified based on biochemical and serological tests as well as matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) profile...
April 30, 2017: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/28456552/subtyping-of-canadian-isolates-of-salmonella-enteritidis-using-multiple-locus-variable-number-tandem-repeat-analysis-mlva-alone-and-in-combination-with-pulsed-field-gel-electrophoresis-pfge-and-phage-typing
#15
Kim Ziebell, Linda Chui, Robin King, Suzanne Johnson, Patrick Boerlin, Roger P Johnson
Salmonella enterica subspecies enterica serovar Enteritidis (SE) is one of the most common causes of human salmonellosis and in Canada currently accounts for over 40% of human cases. Reliable subtyping of isolates is required for outbreak detection and source attribution. However, Pulsed-Field Gel Electrophoresis (PFGE), the current standard subtyping method for Salmonella spp., is compromised by the high genetic homogeneity of SE. Multiple Locus Variable Number Tandem Repeat Analysis (MLVA) was introduced to supplement PFGE, although there is a lack of data on the ability of MLVA to subtype Canadian isolates of SE...
April 26, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28453774/effect-of-dietary-bacteriophage-supplementation-on-internal-organs-fecal-excretion-and-ileal-immune-response-in-laying-hens-challenged-by-salmonella-enteritidis
#16
P A Adhikari, D E Cosby, N A Cox, J H Lee, W K Kim
With the current researches on replacing antibiotics with different dietary interventions, bacteriophages (BP) are potential antimicrobial intervention because of their ability to affect specific bacteria. A study was conducted to evaluate the role of BP against Salmonella enterica serovar Enteritidis (SE) on SE internal organs colonization and ileum immune response in laying hens. Hens were challenged both orally and intracloacally with 108 cfu/mL cells of nalidixic acid resistant Salmonella Enteritidis (SENAR)...
April 26, 2017: Poultry Science
https://www.readbyqxmd.com/read/28451535/simultaneous-molecular-detection-of-salmonella-enterica-serovars-typhi-enteritidis-infantis-and-typhimurium
#17
Reza Ranjbar, Seyyed Mojtaba Mortazavi, Ali Mehrabi Tavana, Meysam Sarshar, Ali Najafi, Rahim Soruri Zanjani
BACKGROUND: Salmonella enterica serovar Typhi, as causative agent of typhoid fever, is one of the most important endemic pathogens. Non-typhoidal Salmonella serovars, including Typhimurium, Infantis, and Enteritidis are amongst the most prevalent serotypes worldwide and in developing areas such as Iran. The aim of this study was to apply a uniplex PCR for rapid detection of Salmonella spp., and a multiplex PCR for the simultaneous detection of the four most common Salmonella serovars in Iran...
January 2017: Iranian Journal of Public Health
https://www.readbyqxmd.com/read/28447329/immune-response-against-salmonella-enteritidis-is-unsettled-by-hiv-infection
#18
Maira Costa Cacemiro, Milena Sobral Espíndola, Leonardo Judson Galvão-Lima, Luana Silva Soares, Caroline Fontanari, Marco Aurélio Prata, Fábio Campioni, Juliana Pfrimer Falcão, Valdes Roberto Bollela, Fabiani Gai Frantz
The human immunodeficiency virus (HIV) is responsible for more than 2 million new infections per year and opportunistic infections such as Salmonella spp. Gastroenteritis is an important cause of mortality and morbidity in developing countries. Monocytes and macrophages play a critical role in the innate immune response against bacterial infections. However during HIV infection the virus can infect these cells and although they are more resistant to the cytopathic effects, they represent an important viral reservoir in these patients...
April 27, 2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28443586/salmonella-enterica-serovar-enteritidis-liver-abscess-after-blunt-abdominal-trauma
#19
Agata Ladic, Igor Petrovic, Ante Gojevic, Emil Kinda, Ivo Coza
BACKGROUND: Salmonella enterica serovar Enteritidis is among the most reported serotypes of Salmonella species worldwide, but rarely reported as the causative agent of a liver abscess. CASE REPORT: We present a patient with an abdominal blunt trauma. An initial abdominal CT scan revealed rupture of the right kidney and of the liver. Two days later, his physical state deteriorated and a new CT scan was obtained. An eight-centimetre large liver abscess was verified...
April 13, 2017: Balkan Medical Journal
https://www.readbyqxmd.com/read/28443289/frequency-and-duration-of-fecal-shedding-of-salmonella-enteritidis-by-experimentally-infected-laying-hens-housed-in-enriched-colony-cages-at-different-stocking-densities
#20
Richard K Gast, Rupa Guraya, Deana R Jones, Kenneth E Anderson, Darrin M Karcher
Human infections with Salmonella Enteritidis are often attributed to the consumption of contaminated eggs, so the prevalence of this pathogen in egg-laying poultry is an important public health risk factor. Numerous and complex environmental influences on Salmonella persistence and transmission are exerted by management practices and housing facilities used in commercial egg production. In recent years, the animal welfare implications of poultry housing systems have guided the development of alternatives to traditional cage-based housing, but their food safety consequences are not yet fully understood...
2017: Frontiers in Veterinary Science
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