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Matthew A Croxen, Kimberley A Macdonald, Matthew Walker, Nancy deWith, Erin Zabek, Christy Peterson, Aleisha Reimer, Linda Chui, Lorelee Tschetter, Linda Hoang, Robin K King
BACKGROUND: A multi-provincial outbreak of Salmonella enterica serovar Enteritidis was linked to newly hatched chicks and poults from a single hatchery during the spring of 2015. In total, there were 61 human cases that were epidemiologically confirmed to be linked to the chicks and poults and the outbreak was deemed to have ended in the summer of 2015. METHODS: PulseNet Canada, in coordination with the affected provinces, used genome sequencing of human and agricultural Salmonella Enteritidis isolates to aid in the epidemiological investigation, while also using traditional typing methods such as phagetyping and pulsed-field gel electrophoresis (PFGE)...
August 9, 2017: PLoS Currents
Teruyo Ojima-Kato, Naomi Yamamoto, Satomi Nagai, Keisuke Shima, Yumi Akiyama, Junji Ota, Hiroto Tamura
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS)-based microbial identification is a popular analytical method. Strain Solution proteotyping software available for MALDI-TOF MS has great potential for the precise and detailed discrimination of microorganisms at serotype- or strain-level, beyond the conventional mass fingerprinting approaches. Here, we constructed a theoretically calculated mass database of Salmonella enterica subspecies enterica consisting of 12 biomarker proteins: ribosomal proteins S8, L15, L17, L21, L25, and S7, Mn-cofactor-containing superoxide dismutase (SodA), peptidyl-prolyl cis-trans isomerase C (PPIase C), and protein Gns, and uncharacterized proteins YibT, YaiA, and YciF, that can allow serotyping of Salmonella...
October 14, 2017: Applied Microbiology and Biotechnology
Nadine Yossa, Gabriela Arce, James Smiley, Mei-Chiung Jo Huang, Lanlan Yin, Rebecca Bell, Sandra Tallent, Eric Brown, Thomas Hammack
OBJECTIVE: Pathogenic contamination of cosmetics intended to be applied on or around the eye area, including make-up removers, may lead to severe eye infections. To assess the efficacy of antimicrobial preservatives in these products we investigated the survival and detection of Bacillus cereus F 4227A spiked into make-up removers, alone and in the presence of other relevant microorganisms. METHODS: Four brands of make-up removers, A, B, C and D, were challenged three times (day 0, day 7, and day 14) using B...
October 14, 2017: International Journal of Cosmetic Science
Marsha Taylor, William Cox, Michael Otterstatter, Nancy de With, Eleni Galanis
A collaborative investigation between public health and animal health led to numerous interventions along the food chain in response to an outbreak of human salmonellosis and increased incidence of Salmonella Enteritidis (SE) among poultry. Incidence of both human and chicken SE decreased substantially in 2012 and 2013 following these interventions. We used time series analysis to assess the impact of three interventions: vaccination of broiler breeder flocks, separation in the hatchery of breeder eggs, and an industry order to stop farm-gate sales of ungraded broiler hatching eggs...
October 13, 2017: Foodborne Pathogens and Disease
Yeon Jo Ha, Sam Woong Kim, Chae Won Lee, Chang-Hwan Bae, Joo-Hong Yeo, Il-Suk Kim, Sang Wan Gal, Jin Hur, Ho-Kyoung Jung, Min-Ju Kim, Woo Young Bang
A previous study highlighted that mastoparan V1 (MP-V1), a mastoparan from the venom of the social wasp Vespula vulgaris, is a potent antimicrobial peptide against Salmonella infection, which causes enteric diseases. However, there exist some limits for its practical application due to the loss of its activity in an increased bacterial density and the difficulty of its efficient production. In this study, we first modulated successfully the antimicrobial activity of synthetic MP-V1 against an increased Salmonella population using protease inhibitors, and developed an Escherichia coli secretion system efficiently producing active MP-V1...
October 13, 2017: Toxins
Kun Jia, Guangyu Wang, Lijiao Liang, Meng Wang, Huhu Wang, Xinglian Xu
Salmonella has emerged as a well-recognized food-borne pathogen, with many strains able to form biofilms and thus cause cross-contamination in food processing environments where acid-based disinfectants are widely encountered. In the present study, RNA sequencing was employed to establish complete transcriptome profiles of Salmonella Enteritidis in the forms of planktonic and biofilm-associated cells cultured in Tryptic Soytone Broth (TSB) and acidic TSB (aTSB). The gene expression patterns of S. Enteritidis significantly differed between biofilm-associated and planktonic cells cultivated under the same conditions...
2017: Frontiers in Microbiology
Anup Kollanoor Johny, Jonathan G Frye, Annie Donoghue, Dan J Donoghue, Steffen Porwollik, Michael McClelland, Kumar Venkitanarayanan
Background:Salmonella Enteritidis phage type 8 (PT8) is a major poultry-associated Salmonella strain implicated in foodborne outbreaks in the United States. We previously reported that two plant-derived compounds generally recognized as safe (GRAS), trans-cinnamaldehyde (TC), and eugenol (EG), significantly reduced S. Enteritidis colonization in broiler and layer chickens. To elucidate potential PT8 genes affected by TC and EG during colonization, a whole-genome microarray analysis of the bacterium treated with TC and EG was conducted...
2017: Frontiers in Microbiology
Derya Savran, Fernando Pérez-Rodríguez, A Kadir Halkman
The objective of this study was to evaluate the behavior of Salmonella Enteritidis and Salmonella Typhimurium, the two most important serovars of salmonellosis , during the fermentation of yogurt. The microorganisms were enumerated in milk throughout the fermentation process at three initial inoculum levels (3, 5 and 7 log CFU/mL). DMFit software was used in the fitting procedure of the data (IFR, Norwich, UK, Version 3.5). The data provided sigmoidal curves that were successfully displayed with the Baranyi model...
January 1, 2017: Food Science and Technology International, Ciencia y Tecnología de Los Alimentos Internacional
Atieh Hashemi, Fahimeh Baghbani-Arani, Sepideh Ahmadiyan, Sarvenaz Ghavami-Nejad
Due to the limitations of serotyping, to differentiate closely related microbial isolates and to investigate disease outbreaks, molecular genotyping methods including multiple loci variable number of tandem repeats (VNTR) analysis (MLVA) has been developed. The usefulness of MLVA was recently demonstrated for Salmonella Infantis and Salmonella Enteritidis isolated from human sources in Iran. In the present study. The discriminatory ability of this method was investigated in 78 Iranian Salmonella enterica isolates...
October 5, 2017: Microbial Pathogenesis
U Methner, A Berndt, M Locke
A live Salmonella Enteritidis vaccine (SE147N ΔphoP fliC), able to express both a homologous intestinal colonisation-inhibition effect and a systemic invasion-inhibition effect, was tested for its potential to generate a postulated additive protective effect in case of combined application with a competitive exclusion (CE) culture against Salmonella exposure in very young chicks. Both, SE147N ΔphoP fliC and the CE culture alone were highly protective against systemic and intestinal colonisation of the challenge strain in case of moderate Salmonella exposure, consequently, additive protective effects in combined use could not be detected...
October 27, 2017: Vaccine
Florian Schenk, Patricia Weber, Julian Vogler, Lars Hecht, Andreas Dietzel, Günter Gauglitz
Lateral flow type detection is becoming interesting not only in regions with a poor medical infrastructure but also for practitioners in day-to-day clinical work or for veterinary control in case of possible epidemics. In this work, we describe the first steps of development of a multi-channel strip with potential internal calibration of multiparametric and colorimetric lateral flow assays for the simultaneous detection of the lipopolysaccharides (LPS) of Salmonella typhimurium (S. typhimurium) and Salmonella enteritidis (S...
October 2, 2017: Analytical and Bioanalytical Chemistry
Munyadziwa Muvhali, Anthony Marius Smith, Andronica Moipone Rakgantso, Karen Helena Keddy
BACKGROUND: Salmonella enterica serovar Enteritidis (Salmonella Enteritidis) has become a significant pathogen in South Africa, and the need for improved molecular surveillance of this pathogen has become important. Over the years, multi-locus variable-number tandem-repeats analysis (MLVA) has become a valuable molecular subtyping technique for Salmonella, particularly for highly homogenic serotypes such as Salmonella Enteritidis. This study describes the use of MLVA in the molecular epidemiological investigation of outbreak isolates in South Africa...
October 2, 2017: BMC Infectious Diseases
Cyrelys Collazo, Maribel Abadías, Pilar Colás-Medà, María Belén Iglesias, Ana Belén Granado-Serrano, José Serrano, Inmaculada Viñas
To further gain insight into the mechanism by which the biopreservative bacterium Pseudomonas graminis CPA-7 develops its antimicrobial activity, we have examined the effect that the prior interaction stablished by this bacterium and two foodborne pathogens on fresh-cut pear, has on their capacity to colonize human epithelial cells (Caco-2 cell line) which is crucial for establishing infection. CPA-7 inhibited the growth of L. monocytogenes and S. enterica subsp. enterica ser. Enteritidis by 5.5 and 3.1 log10, respectively, after 7d of interaction at 10°C...
December 4, 2017: International Journal of Food Microbiology
Nagarajan Vinod, Han Byul Noh, Sung Oh, Seongmi Ji, Hyun Jung Park, Ki-Sung Lee, Sei Chang Kim, Han-Oh Park, Joo-Sung Yang, Chang Won Choi
Salmonella enteritidis and Salmonella typhimurium are important food-borne bacterial pathogens, which are responsible for diarrhea and gastroenteritis in humans and animals. In this study, S. typhimurium bacterial ghost (STG) was generated based on minimum inhibitory concentration (MIC) of sodium hydroxide (NaOH). Experimental studies performed using in vitro and in vivo experimental model systems to characterize effects of STG as a vaccine candidate. When compared with murine macrophages (RAW 264.7) exposed to PBS buffer (98...
2017: PloS One
Yongxiang Wang, Anyun Zhang, Yongqiang Yang, Changwei Lei, Wei Jiang, Bihui Liu, Hongping Shi, Linghan Kong, Guangyang Cheng, Xiuzhong Zhang, Xin Yang, Hongning Wang
The aim of this study was to investigate the prevalence and characterization of Salmonella concerning the poultry industry in China. A total of 170 non-duplicate Salmonella isolates were recovered from the 1540 chicken samples. Among the Salmonella isolates from chickens, the predominant serovars were S. enterica serovar Enteritidis (S. Enteritidis) (49/170, 28.8%), S. enterica serovar Indiana (S. Indiana) (37/170, 21.8%) and S. enterica serovar California (S. California) (34/170, 20.0%). High antimicrobial resistance was observed for ciprofloxacin (68...
December 4, 2017: International Journal of Food Microbiology
Thomas N Denagamage, Bhushan M Jayarao, Eva Wallner-Pendleton, Paul H Patterson, Subhashinie Kariyawasam
Consumption of shell eggs has been associated with Salmonella Enteritidis (SE) infections in humans in the United States. Because of this, the Pennsylvania Egg Quality Assurance Program (PEQAP) was developed and implemented in 1994. The PEQAP involves periodic flock testing and management practices to minimize SE contamination of shell eggs. Subsequently, the U.S. Food and Drug Administration (FDA) introduced a mandatory federal program in 2010 and 2012 for shell egg producers modeled closely after PEQAP to reduce the incidence and prevalence of SE during production, storage, and transport nationwide...
September 2017: Avian Diseases
Richard K Gast, Rupa Guraya, Deana R Jones, Jean Guard, Kenneth E Anderson, Darrin M Karcher
Eggs contaminated with Salmonella Enteritidis are leading sources of human salmonellosis, but Salmonella Heidelberg and Salmonella Typhimurium are also egg-associated pathogens. The management practices and housing facilities characterizing different systems for housing commercial egg flocks can influence Salmonella persistence and transmission. Animal welfare aspects of poultry housing have been widely debated, but their food safety ramifications are not thoroughly understood. The present study assessed the effects of two different bird stocking densities on the frequency and duration of fecal shedding of strains of Salmonella Heidelberg and Salmonella Typhimurium in groups of experimentally infected laying hens housed in colony cages enriched with perching and nesting areas...
September 2017: Avian Diseases
Kazuhisa Tomita, Jun Sawai
 The inhibition of microbial attachment to food is important for the prevention of cross-contamination during food processing. The effect of several chemicals that were added in an Escherichia coli growth medium on the attachment of the bacterium to lettuce was investigated. E. coli ATCC 25922, which is reportedly a useful surrogate for E. coli O157:H7 in surface attachment studies, was preincubated in a nutrient broth (NB) containing sodium chloride, potassium chloride, sodium deoxycholate, sodium linear alkylbenzene sulfonate, or sorbic acid...
2017: Biocontrol Science
Jorge Cabezas-Pizarro, Mauricio Redondo-Solano, Christian Umaña-Gamboa, María Laura Arias-Echandi
Cleaning and disinfection represent the most important activities associated with the elimination of dirt and microorganisms at food processing plants. Improper procedures may lead to cross contamination of food leading to its spoilage or even the transmission of foodborne pathogens. Several strategies have been used in order to achieve a good disinfection of surfaces and products; nevertheless, microbial resistance to common-use-products has developed lately. Due to this fact, the development of new non-toxic-food compatible chemical agents that reduce the impact of foodborne pathogens and spoilage causing microorganisms is desirable for the food industry...
September 22, 2017: Revista Argentina de Microbiología
Vuk Uskoković, Maheshwar Adiraj Iyer, Victoria M Wu
Although hydroxyapatite (HAp) has been doped with dozens of different ions, the quest for an ion imparting a combination of properties conducive to bone healing is still ongoing. Because of its protean potency and the similarity in size and shape to the phosphate tetrahedron, selenite ion presents a natural ionic substitute in HAp. The incorporation of selenite into synthetic HAp using two different methods - co-precipitation and ion-exchange sorption - was studied for its effect on crystal properties and on a triad of biological responses: antibacterial, anticancer and osteoinductive...
2017: Journal of Materials Chemistry. B, Materials for Biology and Medicine
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