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https://www.readbyqxmd.com/read/28735524/characterization-of-cytoskeleton-features-and-maturation-status-of-cultured-human-ipsc-derived-cardiomyocytes
#1
Christian Zuppinger, George Gibbons, Priyanka Dutta-Passecker, Adrian Segiser, Henriette Most, Thomas M Suter
Recent innovations in stem cell technologies and the availability of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) have opened new possibilities for studies and drug testing on human cardiomyocytes in vitro. Still, there are concerns about the precise nature of such 'reprogrammed' cells. We have performed an investigation using immunocytochemistry and confocal microscopy on several cellular features using commercially available hiPSC-CMs. For some selected developmentally regulated or cardiac chamber-specific proteins, we have compared the results from hiPSC-derived cardiomyocytes with freshly isolated, ventricular cardiomyocytes from adult rats...
June 21, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28735516/dnmt1-regulates-il-6-and-tgf-%C3%AE-1-induced-epithelial-mesenchymal-transition-in-prostate-epithelial-cells
#2
Hui Xu, Yanbo Chen, Qi Chen, Huan Xu, Yanxia Wang, Jiao Yu, Juan Zhou, Zhong Wang, Bing Xu
Multiple factors have been considered to play a role in the development of benign prostatic hyperplasia (BPH), including chronic inflammation, hormones, and epithelial-mesenchymal transition (EMT). Inflammation is regarded as one of the potential inducers of EMT. However, the mechanisms, modulating pro-inflammatory factors (IL-6 and TGF-β1) induce EMT features, have not yet been studied in BPH. In this study, we investigated whether DNA methyltransferases1 (DNMT1) could regulate IL-6 and TGF-β1 induce EMT...
May 3, 2017: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/28735480/immunophenotyping-by-multiparameter-flow-cytometry
#3
Weina Chen, Hung S Luu
Multiparameter flow cytometry has become an indispensable tool for the diagnosis and classification of acute myeloid leukemia (AML). The basic method relies on the unique ability to detect immunophenotypic abnormalities on discrete subpopulations. The primary roles in the initial assessment of AML are to determine the immaturity of the leukemic population, define the lineage and the immunophenotypic aberrancies in blasts, and identify characteristic immunophenotypic features to predict important recurrent cytogenetic and genetic abnormalities and prognosis...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28735479/immunohistochemistry-in-acute-myeloid-leukemia
#4
Michael W Cruise
The World Health Organization's Classification of Tumours of Haematopoietic and Lymphoid Tissues (Swerdlow et al. (eds) WHO Classification of tumours of haematopoietic and lymphoid tissues, 4th edn. WHO Press, Lyon, 2008) created a classification scheme incorporating genetic, molecular, morphologic, and immunophenotypic characteristics. The diagnosis of acute myeloid leukemia requires equal to or greater than 20% blasts (except in some cases with specific cytogenetic abnormalities or in erythroleukemia). The diagnostic work up typically includes morphologic, cytochemical, and immunophenotypic features...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28735439/surgical-management-of-periocular-cancers-high-and-low-risk-features-drive-treatment
#5
REVIEW
Richard C Allen
PURPOSE OF REVIEW: Recent advances in the treatment of eyelid tumors have centered on the excision, evaluation of margins, role of sentinel lymph node biopsy, and adjunctive/adjuvant systemic and radiation therapy. The purpose of this review is to elaborate on these advances. RECENT FINDINGS: Mohs excision of basal cell carcinoma and squamous cell carcinoma continues to provide the greatest success in complete excision of the cancer, especially in those cases of high-risk disease including medial canthal location and recurrent disease...
September 2017: Current Oncology Reports
https://www.readbyqxmd.com/read/28735407/determination-of-polyamines-by-dansylation-benzoylation-and-capillary-electrophoresis
#6
Gounipalli Veeranagamallaiah, Chinta Sudhakar
Polyamines are polycationic nitrogenous compounds that accumulate in plants exposed to abiotic stresses. In higher animals, they influence gene expression, brain development, and nerve growth and regeneration. Because of their known roles in plant stress responses, quantitative determination of polyamines is very important. Polyamines in their native form cannot be detected by optical and/or electrochemical methods as they do not show any structural features, and hence derivatization of polyamines is essential to make them to produce either chromophores or fluorescence...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28735359/binding-abstract-concepts
#7
Tarini Singh, Christian Frings, Birte Moeller
Binding theories assume that a stimulus and the response made to it are bound together in an event file (Hommel et al., Behav Brain Sci 24(05):849-937, 2001). Such bindings can occur even after single encounters. If the stimulus or parts of its features are repeated within the time frame in which the event file is still intact, the previously integrated response is retrieved. Stimulus-response binding can exist at a perceptual, conceptual or a response selection level (Henson et al., Trends Cogn Sci 18(7):376-384, 2014)...
July 22, 2017: Psychological Research
https://www.readbyqxmd.com/read/28735350/inflammatory-joint-disorders-and-neutrophilic-dermatoses-a-comprehensive-review
#8
REVIEW
Massimo Cugno, Roberta Gualtierotti, Pier Luigi Meroni, Angelo Valerio Marzano
Rheumatoid arthritis and spondyloarthritis are inflammatory joint disorders with an autoimmune pathogenesis and systemic involvement. The skin is one of the most frequently affected extraarticular sites with a number of manifestations or distinct diseases, including common conditions, such as rheumatoid nodules and psoriasis, and rare diseases like neutrophilic dermatoses. The latter are clinically characterised by polymorphic lesions, including pustules, bullae, abscesses, papules, nodules, plaques and ulcers, and histologically by neutrophil-rich inflammatory infiltrates...
July 22, 2017: Clinical Reviews in Allergy & Immunology
https://www.readbyqxmd.com/read/28735330/expiration-date-prediction-of-biocontrol-agent-prepared-with-bacillus-subtilis-b579-using-the-accelerated-aging-method
#9
Yu Zheng, Xiaoyang Jiao, Fang Chen, Xuelian Wang, Min Wang
The expiration date of biopesticidal products is an essential feature of their use and storage. In the present work, the expiration date of biocontrol agent was predicted using the accelerated aging method. The available bacteria in Bacillus subtilis B579 biocontrol agent were 3.7 ± 0.2 × 10(11) CFU/g. It is calculated that the expiration date of the agent was about 17 months at 25°C. During this period, the available bacteria retained more than 90% of the value in the initial product. Thus, this work suggests the expiration date of biocontrol agents composed with spores could be estimated using the accelerated aging method...
January 2, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28735298/foxp1-related-intellectual-disability-syndrome-a-recognisable-entity
#10
Ilse Meerschaut, Daniel Rochefort, Nicole Revençu, Justine Pètre, Christina Corsello, Guy A Rouleau, Fadi F Hamdan, Jacques L Michaud, Jenny Morton, Jessica Radley, Nicola Ragge, Sixto García-Miñaúr, Pablo Lapunzina, Maria Palomares Bralo, Maria Ángeles Mori, Stéphanie Moortgat, Valérie Benoit, Sandrine Mary, Nele Bockaert, Ann Oostra, Olivier Vanakker, Milen Velinov, Thomy Jl de Ravel, Djalila Mekahli, Jonathan Sebat, Keith K Vaux, Nataliya DiDonato, Andrea K Hanson-Kahn, Louanne Hudgins, Bruno Dallapiccola, Antonio Novelli, Luigi Tarani, Joris Andrieux, Michael J Parker, Katherine Neas, Berten Ceulemans, An-Sofie Schoonjans, Darina Prchalova, Marketa Havlovicova, Miroslava Hancarova, Magdalena Budisteanu, Annelies Dheedene, Björn Menten, Patrick A Dion, Damien Lederer, Bert Callewaert
BACKGROUND: Mutations in forkhead box protein P1 (FOXP1) cause intellectual disability (ID) and specific language impairment (SLI), with or without autistic features (MIM: 613670). Despite multiple case reports no specific phenotype emerged so far. METHODS: We correlate clinical and molecular data of 25 novel and 23 previously reported patients with FOXP1 defects. We evaluated FOXP1 activity by an in vitro luciferase model and assessed protein stability in vitro by western blotting...
July 22, 2017: Journal of Medical Genetics
https://www.readbyqxmd.com/read/28735260/aetiological-investigations-in-early-developmental-impairment-are-they-worth-it
#11
Anthony Richard Hart, Ruchi Sharma, Mark Atherton, Samer Alabed, Sally Simpson, Stuart Barfield, Judith Cohen, Nicholas McGlashan, Asha Ravi, Michael James Parker, Daniel Ja Connolly
OBJECTIVE: To study the frequency a diagnosis is made in children with early developmental impairment (EDI), and the contribution made to diagnosis by specific investigations. DESIGN: Retrospective case note review. SETTING: Community, neurodisability and neurology department at a UK tertiary centre. PARTICIPANTS: Children referred to determine the aetiology of EDI where a cause was not evident on history and examination...
July 22, 2017: Archives of Disease in Childhood
https://www.readbyqxmd.com/read/28735222/effect-of-oleic-acid-on-the-properties-of-protein-adsorbed-layers-at-water-oil-interfaces-an-epr-study-combined-with-dynamic-interfacial-tension-measurements
#12
Eleni P Kalogianni, Leandros Sklaviadis, Stella Nika, Ioanna Theochari, Georgia Dimitreli, Despoina Georgiou, Vassiliki Papadimitriou
Multiphase food systems consist of complex interfacial layers where surface active molecules complete compete for adsorption and interact with one another affecting the interfacial properties and the behavior of the food systems involved. The present work focuses on the examination of interactions between proteins playing an which play an important role in milk-rich food systems (namely κ-casein and β-lactoglobulin) and oleic acid (an anionic surfactant abundant in food systems) at the oil/water interface...
July 14, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28735218/strength-of-linguistic-text-evidence-a-fused-forensic-text-comparison-system
#13
Shunichi Ishihara
Compared to other forensic comparative sciences, studies of the efficacy of the likelihood ratio (LR) framework in forensic authorship analysis are lagging. An experiment is described concerning the estimation of strength of linguistic text evidence within that framework. The LRs were estimated by trialling three different procedures: one is based on the multivariate kernel density (MVKD) formula, with each group of messages being modelled as a vector of authorship attribution features; the other two involve N-grams based on word tokens and characters, respectively...
July 8, 2017: Forensic Science International
https://www.readbyqxmd.com/read/28735197/synthesis-of-porous-polymer-based-solid-amine-adsorbent-effect-of-pore-size-and-amine-loading-on-co2-adsorption
#14
Fenglei Liu, Shuixia Chen, Yanting Gao
A series of porous polymers was synthesized by a suspension polymerization of divinylbenzene (DVB) and ethylene glycol dimethyl acrylate (EGDMA), which was further functionalized with polyethyleneimine (PEI) for CO2 capture. The results showed that the synthesized DVB and EGDMA (DE) copolymers were an effective support for loading PEI because of its larger pore size and specific surfaces area. It was found that DE (30, 10) loaded with 30wt% PEI exhibited a higher CO2 adsorption amount of 3.28mmol/g at 25°C under dry condition...
July 17, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28735174/tetherless-near-infrared-control-of-brain-activity-in-behaving-animals-using-fully-implantable-upconversion-microdevices
#15
Ying Wang, Xudong Lin, Xi Chen, Xian Chen, Zhen Xu, Wenchong Zhang, Qinghai Liao, Xin Duan, Xin Wang, Ming Liu, Feng Wang, Jufang He, Peng Shi
Many nanomaterials can be used as sensors or transducers in biomedical research and they form the essential components of transformative novel biotechnologies. In this study, we present an all-optical method for tetherless remote control of neural activity using fully implantable micro-devices based on upconversion technology. Upconversion nanoparticles (UCNPs) were used as transducers to convert near-infrared (NIR) energy to visible light in order to stimulate neurons expressing different opsin proteins. In our setup, UCNPs were packaged in a glass micro-optrode to form an implantable device with superb long-term biocompatibility...
July 12, 2017: Biomaterials
https://www.readbyqxmd.com/read/28735144/entorhinal-tau-pathology-disrupts-hippocampal-prefrontal-oscillatory-coupling-during-associative-learning
#16
Stephanie E Tanninen, Bardia Nouriziabari, Mark D Morrissey, Rami Bakir, Robert D Dayton, Ronald L Klein, Kaori Takehara-Nishiuchi
A neural signature of asymptomatic preclinical Alzheimer's disease (AD) is disrupted connectivity between brain regions; however, its underlying mechanisms remain unknown. Here, we tested whether a preclinical pathologic feature, tau aggregation in the entorhinal cortex (EC) is sufficient to disrupt the coordination of local field potentials (LFPs) between its efferent regions. P301L-mutant human tau or green fluorescent protein (GFP) was virally overexpressed in the EC of adult rats. LFPs were recorded from the dorsal hippocampus and prelimbic medial prefrontal cortex while the rats underwent trace eyeblink conditioning where they learned to associate 2 stimuli separated by a short time interval...
July 6, 2017: Neurobiology of Aging
https://www.readbyqxmd.com/read/28735125/management-of-arteriovenous-malformations-associated-with-developmental-venous-anomalies-a-literature-review-and-report-of-two-cases
#17
Michael Zhang, Ian D Connolly, Mario K Teo, George Yang, Robert Dodd, Michael Marks, Mario Zuccarello, Gary K Steinberg
OBJECTIVE: Cerebrovascular malformation classification has revealed intermediary lesions that warrant further review due to their unusual presentation and management. We present two cases of arteriovenous malformations (AVMs) associated with a developmental venous anomaly (DVA), and discuss the efficacy of previously published management strategies. METHODS: Two cases of AVMs associated with DVA were identified and a literature search for published cases between 1980 and 2016 was conducted...
July 19, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/28735087/fisher-kpp-with-time-dependent-diffusion-is-able-to-model-cell-sheet-activated-and-inhibited-wound-closure
#18
Boutheina Yahyaoui, Mekki Ayadi, Abderrahmane Habbal
The popular 2D Fisher-KPP equation with constant parameters fails to predict activated or inhibited cell-sheet wound closure. Here, we consider the case where the collective diffusion coefficient is time dependent, with a 3-parameter sigmoid profile. The sigmoid is taken S-shaped for the activated assays, and Z-shaped for the inhibited ones. For two activated and two inhibited assays, our model is able to predict with a very good accuracy features of the wound closure like as the time evolution of the wound area and migration rate...
July 19, 2017: Mathematical Biosciences
https://www.readbyqxmd.com/read/28735061/up-regulation-of-glur1-in-paraventricular-nucleus-and-greater-expressions-of-synapse-related-proteins-in-the-hypothalamus-of-chronic-unpredictable-stress-induced-depressive-rats
#19
Rong Li, Xia Zhao, Li Cai, Wan-Wan Gao
Converging evidence indicates that abnormal glutamatergic and synaptic systems may be associated with the pathophysiology of depression. Over-activation of corticotropin-releasing hormone (CRH) neurons in the hypothalamic paraventricular nucleus (PVN) plays a key role in the hypothalamic-pituitary-adrenal (HPA) axis hyperactivity and is a prominent feature in depression. In this study, we examined the effect of chronic unpredictable stress (CUS) in rats on the expression of AMPA subunit GluR1 protein in the hypothalamic PVN as well as on the expression of GluR1 and several synapse-related proteins in the hypothalamus...
July 19, 2017: Physiology & Behavior
https://www.readbyqxmd.com/read/28735016/timing-of-psa-nadir-after-radical-prostatectomy-and-risk-of-biochemical-recurrence
#20
Stephanie L Skove, Lauren E Howard, William J Aronson, Martha K Terris, Christopher J Kane, Christopher L Amling, Matthew R Cooperberg, Daniel M Moreira, Stephen J Freedland
OBJECTIVES: To evaluate the association between PSA nadir level and time to nadir (TTN) with biochemical recurrence (BCR) risk after radical prostatectomy (RP) in the SEARCH database. METHODS: Retrospective analysis of 1,939 men from the SEARCH database treated with RP between 1998-2015 with available ultrasensitive PSA nadir within 1-6 months after RP. Uni- and multivariable analyses of PSA nadir and TTN with time from nadir to BCR were done with Cox models (adjusted for demographics, tumor features and preoperative PSA)...
July 19, 2017: Urology
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