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https://www.readbyqxmd.com/read/29052187/sequential-chip-protocol-for-profiling-bivalent-epigenetic-modifications-rechip
#1
Bénédicte Desvoyes, Joana Sequeira-Mendes, Zaida Vergara, Sofia Madeira, Crisanto Gutierrez
Identification of chromatin modifications, e.g., histone acetylation and methylation, among others, is widely carried out by using a chromatin immunoprecipitation (ChIP) strategy. The information obtained with these procedures is useful to gain an overall picture of modifications present in all cells of the population under study. It also serves as a basis to figure out the mechanisms of chromatin organization and gene regulation at the population level. However, the ultimate goal is to understand gene regulation at the level of single chromatin fibers...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29052186/a-rapid-and-efficient-chip-protocol-to-profile-chromatin-binding-proteins-and-epigenetic-modifications-in-arabidopsis
#2
Bénédicte Desvoyes, Zaida Vergara, Joana Sequeira-Mendes, Sofia Madeira, Crisanto Gutierrez
Chromatin immunoprecipitation (ChIP) is a widely used and very powerful procedure to identify the proteins that are associated with the DNA to regulate developmental processes. These proteins can be transcription factors, or specific histone variants and modified histones, which are all crucial for gene regulation. In order to obtain reliable results, ChIP must be carried out under highly reproducible conditions. Here, we describe a simple and fast ChIP protocol adapted for Arabidopsis seedlings, which can serve as a basis for other species, organs or more sophisticated procedures, such as the sequential ChIP...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29050202/phosphodiesterase-10a-is-overexpressed-in-lung-tumor-cells-and-inhibitors-selectively-suppress-growth-by-blocking-%C3%AE-catenin-and-mapk-signaling
#3
Bing Zhu, Ashley Lindsey, Nan Li, Kevin Lee, Veronica Ramirez-Alcantara, Joshua C Canzoneri, Alexandra Fajardo, Luciana Madeira da Silva, Meagan Thomas, John T Piazza, Larry Yet, Brian T Eberhardt, Evrim Gurpinar, Dennis Otali, William Grizzle, Jacob Valiyaveettil, Xi Chen, Adam B Keeton, Gary A Piazza
Phosphodiesterase 10A (PDE10) is a cyclic nucleotide (e.g. cGMP) degrading enzyme highly expressed in the brain striatum where it plays an important role in dopaminergic neurotransmission, but has limited expression and no known physiological function outside the central nervous system. Here we report that PDE10 mRNA and protein levels are strongly elevated in human non-small cell lung cancer cells and lung tumors compared with normal human airway epithelial cells and lung tissue, respectively. Genetic silencing of PDE10 or inhibition by small molecules such as PQ10 was found to selectively inhibit the growth and colony formation of lung tumor cells...
September 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29049185/relationship-between-add1-gly460trp-gene-polymorphism-and-essential-hypertension-in-madeira-island
#4
Ana Célia Sousa, Roberto Palma Dos Reis, Andreia Pereira, Sofia Borges, Ana Isabel Freitas, Graça Guerra, Teresa Góis, Mariana Rodrigues, Eva Henriques, Sónia Freitas, Ilídio Ornelas, Décio Pereira, António Brehm, Maria Isabel Mendonça
Essential hypertension (EH) is a complex disease in which physiological, environmental, and genetic factors are involved in its genesis. The genetic variant of the alpha-adducin gene (ADD1) has been described as a risk factor for EH, but with controversial results.The objective of this study was to evaluate the association of ADD1 (Gly460Trp) gene polymorphism with the EH risk in a population from Madeira Island.A case-control study with 1614 individuals of Caucasian origin was performed, including 817 individuals with EH and 797 controls...
October 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29026429/markers-of-neuroprotection-of-combined-epa-and-dha-provided-by-fish-oil-are-higher-than-those-of-epa-nannochloropsis-and-dha-schizochytrium-from-microalgae-oils-in-wistar-rats
#5
Paula A Lopes, Narcisa M Bandarra, Susana V Martins, Joana Martinho, Cristina M Alfaia, Marta S Madeira, Carlos Cardoso, Cláudia Afonso, Maria C Paulo, Rui M A Pinto, José L Guil-Guerrero, José A M Prates
BACKGROUND: To overcome the current overexploitation of fish rich in n-3 long chain polyunsaturated fatty acids (LCPUFA), microalgae have become a promising marine lipid source. The purpose of this study was to assess eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3), isolated or combined from distinct marine origins, on the promotion of neuroprotective effects. METHODS: The experiment lasted for 10 weeks and involved 32 Wistar rats, divided into 4 diets (n = 8): a diet rich in milk fat was taken as control (Milk Fat) and compared to n-3 LCPUFA enriched diets, either in EPA + DHA form through fish oil (Fish Oil), or EPA through Nannochloropsis oil (Nanno), or DHA through Schizochytrium oil (Schyzo), while maintaining Milk Fat incorporation...
2017: Nutrition & Metabolism
https://www.readbyqxmd.com/read/28992572/ecotoxicity-of-the-insensitive-munitions-compound-3-nitro-1-2-4-triazol-5-one-nto-and-its-reduced-metabolite-3-amino-1-2-4-triazol-5-one-ato
#6
Camila L Madeira, Jim A Field, Michael T Simonich, Robert L Tanguay, Jon Chorover, Reyes Sierra-Alvarez
The insensitive munitions compound 3-nitro-1,2,4-triazol-5-one (NTO) was recently approved by the U.S. Army to replace cyclotrimethylene trinitramine (RDX) in conventional explosives. As its use becomes widespread, concern about the potential toxicity of NTO increases. NTO can undergo microbial reduction to 3-amino-1,2,4-triazol-5-one (ATO), which is recalcitrant in waterlogged soils. In this study, the acute toxicity of NTO and ATO towards various organisms, including microorganisms (i.e., methanogenic archaea, aerobic heterotrophs, and Aliivibrio fischeri (Microtox assay)), the microcrustacean Daphnia magna (ATO only), and zebrafish embryos (Danio rerio), was assessed...
September 28, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28983958/management-of-syphilis-in-pregnancy-knowledge-and-practices-of-health-care-providers-and-barriers-to-the-control-of-disease-in-teresina-brazil
#7
Danielle Carvalho Rodrigues, Rosa Maria Soares Madeira Domingues
AIMS: The aim of the study is to verify the knowledge and practices of health professionals working in prenatal care (PNC) related with syphilis during pregnancy and to identify the main barriers to the implementation of protocols for the control of this disease. METHODS: A cross-sectional study in Teresina, Brazil, from January to May 2015, was conducted with 366 physicians and nurses working in PNC, corresponding to 70% of eligible professionals. We evaluated 20 knowledge and practice criteria related to the diagnosis and treatment of syphilis during pregnancy with a 95% compliance standard...
October 6, 2017: International Journal of Health Planning and Management
https://www.readbyqxmd.com/read/28981089/treatment-with-a2a-receptor-antagonist-kw6002-and-caffeine-intake-regulate-microglia-reactivity-and-protect-retina-against-transient-ischemic-damage
#8
Raquel Boia, Filipe Elvas, Maria H Madeira, Inês D Aires, Ana C Rodrigues-Neves, Pedro Tralhão, Eszter C Szabó, Younis Baqi, Christa E Müller, Ângelo R Tomé, Rodrigo A Cunha, António F Ambrósio, Ana R Santiago
Transient retinal ischemia is a major complication of retinal degenerative diseases and contributes to visual impairment and blindness. Evidences indicate that microglia-mediated neuroinflammation has a key role in the neurodegenerative process, prompting the hypothesis that the control of microglia reactivity may afford neuroprotection to the retina against the damage induced by ischemia-reperfusion (I-R). The available therapeutic strategies for retinal degenerative diseases have limited potential, but the blockade of adenosine A2A receptor (A2AR) emerges as candidate strategy...
October 5, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28977093/urinary-en-2-to-predict-prostate-cancer-systematic-review-and-meta-analysis
#9
Maria Inês da Rosa, Eduardo Ronconi Dondossola, Maria Cecilia Manenti Alexandre, Kristian Madeira, Florentino de Araújo Cardoso, Antonio José Grande
INTRODUCTION: Prostate cancer is the second type of cancer diagnosed and the fifth cause of death in men worldwide. Early diagnosis helps to control disease progression. Currently, prostate specific antigen is the standard biomarker, as it has a broad scope of identification and, thus, new and more specific biomarkers must be studied. OBJECTIVE: To evaluate the accuracy of engrailed-2 protein (EN2) in urine as a prostate cancer biomarker. METHOD: A comprehensive search was conducted in the period from January 2005 to July 2016 using the following electronic databases: Medline (PubMed), Embase, Cochrane Library and Lilacs...
July 2017: Revista da Associação Médica Brasileira
https://www.readbyqxmd.com/read/28966601/the-lacanian-concept-of-paranoia-an-historical-perspective
#10
Thomas Lepoutre, Manoel L Madeira, Nicolas Guerin
This article seeks to reopen a major question raised by the Lacanian nosology of the psychoses, by looking closely at Lacan's formulations of what he never ceased referring to as "paranoia". While almost all classification systems of modern psychiatry, such as the ICD-10 and the DSM-5, have abandoned the specific category of paranoia, Lacan always viewed paranoia as a major category of "functional psychosis". He held that paranoia was a qualitatively different disorder than schizophrenia, and considered it to be the principal or exemplary form of psychosis...
2017: Frontiers in Psychology
https://www.readbyqxmd.com/read/28966116/crohn-s-disease-disturbs-the-immune-properties-of-human-adipose-derived-stem-cells-related-to-inflammasome-activation
#11
Carolina Serena, Noelia Keiran, Ana Madeira, Elsa Maymó-Masip, Miriam Ejarque, Margarida Terrón-Puig, Eloy Espin, Marc Martí, Natalia Borruel, Francisco Guarner, Margarida Menacho, Antonio Zorzano, Monica Millan, Sonia Fernández-Veledo, Joan Vendrell
Crohn's disease (CD) is characterized by the expansion of mesenteric fat, also known as "creeping fat." We explored the plasticity and immune properties of adipose-derived stem cells (ASCs) in the context of CD as potential key players in the development of creeping fat. Mesenteric CD-derived ASCs presented a more proliferative, inflammatory, invasive, and phagocytic phenotype than equivalent cells from healthy donors, irrespective of the clinical stage. Remarkably, ASCs from the subcutaneous depot of patients with CD also showed an activated immune response that was associated with a reduction in their immunosuppressive properties...
October 10, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28960302/nutritional-and-phytochemical-composition-of-vaccinium-padifolium-sm-wild-berries-and-radical-scavenging-activity
#12
Maria J Carvalho, Carla S Gouveia, Ana C Vieira, Ana C Pereira, Miguel  Carvalho, José C Marques
Blueberries have a well-deserved reputation as a potential functional food, supported by studies which have identified and quantified various nutrients and bioactive phytochemicals with known benefits for human diet and health. Wild blueberries have attracted particular attention due to the levels and concentrations of those phytonutrients. This study aims to evaluate for the first time the chemical composition of Madeira Island's endemic Vaccinium padifolium Sm wild berry. Results show that this fruit contains high values of total soluble phenolic content (around 4 g GAE kg(-1) FW), as well as significant values of total monomeric anthocyanin content (around 3 g eq...
September 29, 2017: Journal of Food Science
https://www.readbyqxmd.com/read/28959938/genotypic-profiles-of-leishmania-viannia-braziliensis-strains-from-cutaneous-leishmaniasis-patients-and-their-relationship-with-the-response-to-meglumine-antimoniate-treatment-a-pilot-study
#13
Thalita Gagini, Armando de Oliveira Schubach, Maria de Fatima Madeira, Cláudia Maria Valete-Rosalino, Maria Inês Fernandes Pimentel, Raquel da Silva Pacheco
BACKGROUND: Forty-four strains isolated from a cohort of cutaneous leishmaniasis (CL) patients who did or did not respond to one course of treatment with meglumine antimoniate were investigated to explore genetic polymorphisms in parasite kinetoplast DNA minicircles. Leishmania (Viannia) braziliensis strains isolated from responder (R) and non-responder (NR) patients who acquired infection in Rio de Janeiro or in other Brazilian states were studied using low-stringency single-specific primer polymerase chain reaction (LSSP-PCR) to identify genetic polymorphisms...
2017: Parasite: Journal de la Société Française de Parasitologie
https://www.readbyqxmd.com/read/28958439/marine-debris-boost-in-juvenile-magellanic-penguins-stranded-in-south-eastern-brazil-in-less-than-a-decade-insights-into-feeding-habits-and-habitat-use
#14
Ana Paula Madeira Di Beneditto, Salvatore Siciliano
The Magellanic penguin (Spheniscus magellanicus) is a marine sentinel for the southern Atlantic Ocean that is a proxy of environmental quality. The presence of marine debris (macro-debris) in the stomach contents of emaciated juvenile penguins stranded from 21°S to 23°S was compared at different times (2000 and 2008), and the debris ingestion pathway was determined. The frequency of marine debris in the stomachs doubled in less than a decade, and flexible plastics remained the main ingested item over time (68-70%)...
September 25, 2017: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/28954024/assessment-of-eye-drop-instillation-technique-in-glaucoma-patients
#15
Beatriz Fiuza Gomes, Angela Flores Paredes, Nathália Madeira, Haroldo Vieira Moraes, Marcony R Santhiago
Purpose: To study the technique of eye drop instillation in glaucoma patients and identify independent factors that may influence their performance. Methods: In this cross-sectional study, 71 consecutive patients with glaucoma or ocular hypertension, self-administering topical anti-glaucoma medications for ≥6 months were evaluated. All patients instilled a tear substitute into the eye with the worst eyesight using the technique normally used at home. The following parameters were evaluated: age, number of years receiving treatment with ocular hypotensive eye drops, time spent to instill the first drop, number of drops instilled, correct location of the eye drops, contact of the bottle with the eye, closing of the eyelids or occlusion of the tear punctum, and asepsis of the hands...
July 2017: Arquivos Brasileiros de Oftalmologia
https://www.readbyqxmd.com/read/28949190/interatomic-coulombic-decay-the-mechanism-for-rapid-deexcitation-of-hollow-atoms
#16
Richard A Wilhelm, Elisabeth Gruber, Janine Schwestka, Roland Kozubek, Teresa I Madeira, José P Marques, Jacek Kobus, Arkady V Krasheninnikov, Marika Schleberger, Friedrich Aumayr
The impact of a highly charged ion onto a solid gives rise to charge exchange between the ion and target atoms, so that a slow ion gets neutralized in the vicinity of the surface. Using highly charged Ar and Xe ions and the surface-only material graphene as a target, we show that the neutralization and deexcitation of the ions proceeds on a sub-10 fs time scale. We further demonstrate that a multiple Interatomic Coulombic Decay (ICD) model can describe the observed ultrafast deexcitation. Other deexcitation mechanisms involving nonradiative decay and quasimolecular orbital formation during the impact are not important, as follows from the comparison of our experimental data with the results of first-principles calculations...
September 8, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/28947905/urban-dog-parks-as-sources-of-canine-parasites-contamination-rates-and-pet-owner-behaviours-in-lisbon-portugal
#17
Ana Ferreira, Ana Margarida Alho, David Otero, Lídia Gomes, Rolf Nijsse, Paul A M Overgaauw, Luís Madeira de Carvalho
Dog parks represent a recent trend in western countries, enabling owners to spend quality time with their pets in a controlled environment. Despite their growing popularity, few studies have been performed to date on these parks to investigate dog intestinal parasitic infections and soil contamination. The present study examined 369 faecal and 18 soil samples collected from 3 dog parks in Greater Lisbon, Portugal. Additionally, 102 interviews were performed with dog owners to assess dog-walking behaviours and parasite risk...
2017: Journal of Environmental and Public Health
https://www.readbyqxmd.com/read/28930100/the-joule-heating-problem-in-silver-nanowire-transparent-electrodes
#18
H H Khaligh, L Xu, A Khosropour, A Madeira, M Romano, C Pradére, M Tréguer-Delapierre, L Servant, M A Pope, I A Goldthorpe
Silver nanowire transparent electrodes have shown considerable potential to replace conventional transparent conductive materials. However, in this report we show that Joule heating is a unique and serious problem with these electrodes. When conducting current densities encountered in organic solar cells, the average surface temperature of indium tin oxide (ITO) and silver nanowire electrodes, both with sheet resistances of 60 ohms/square, remains below 35 °C. However, in contrast to ITO, the temperature in the nanowire electrode is very non-uniform, with some localized points reaching temperatures above 250 °C...
October 20, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28917210/single-and-mixture-toxicity-of-abamectin-and-difenoconazole-to-adult-zebrafish-danio-rerio
#19
Ana Letícia Madeira Sanches, Bruna Horvath Vieira, Marina Vanderlei Reghini, Raquel Aparecida Moreira, Emanuela Cristina Freitas, Evaldo L G Espíndola, Michiel A Daam
Concerns have been raised in recent years on the potential risks related with pesticide mixtures that are likely to be present in agricultural edge-of-field waterbodies. Despite the high use of pesticides in tropical countries like Brazil, studies evaluating pesticide mixtures are especially scarce in the tropics. The insecticide abamectin and the fungicide difenoconazole are the main pesticides intensively used in Brazilian strawberry crop and are hence likely to occur simultaneously. The aim of the present study was therefore to evaluate the toxicity of abamectin, difenoconazole and their mixture to the tropical fish Danio rerio...
September 6, 2017: Chemosphere
https://www.readbyqxmd.com/read/28916394/a-useful-strategy-based-on-chromatographic-data-combined-with-quality-by-design-approach-for-food-analysis-applications-the-case-study-of-furanic-derivatives-in-sugarcane-honey
#20
Pedro Silva, Catarina L Silva, Rosa Perestrelo, Fernando M Nunes, José S Câmara
Sugarcane honey (SCH) is one of the Madeira Island products par excellence and it is now popular worldwide. Its sui generis and peculiar sensory properties, explained by a variety of volatile compounds including furanic derivatives (FDs), arise mainly from manufacturing and storage conditions. A simple high-throughput approach based on semi-automatic microextraction by packed sorbent (MEPS) combined with ultra-high performance liquid chromatography (UHPLC) was developed and validated for identification and quantification of target FDs in sugarcane honey...
September 8, 2017: Journal of Chromatography. A
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