keyword
MENU ▼
Read by QxMD icon Read
search

frozen stool

keyword
https://www.readbyqxmd.com/read/28531908/methods-and-reporting-studies-assessing-fecal-microbiota-transplantation-a-systematic-review
#1
Aïda Bafeta, Amelie Yavchitz, Carolina Riveros, Rui Batista, Philippe Ravaud
Background: Fecal microbiota transplantation (FMT) could be a novel treatment option for several chronic diseases associated with altered gut microbiota. Purpose: To examine the conduct and reporting of studies assessing FMT. Data Sources: Cochrane Central Register of Controlled Trials, PubMed, EMBASE, and Web of Science from inception to 31 January 2017. Study Selection: Two reviewers independently examined titles and abstracts to identify all English-language reports of human clinical studies assessing the safety or efficacy of FMT...
May 23, 2017: Annals of Internal Medicine
https://www.readbyqxmd.com/read/28529025/how-to-establish-and-run-a-stool-bank
#2
REVIEW
Elisabeth M Terveer, Yvette H van Beurden, Abraham Goorhuis, Jos F M L Seegers, Martijn P Bauer, E van Nood, Marcel G W Dijkgraaf, Chris J J Mulder, Christina M J E Vandenbroucke-Grauls, Hein W Verspaget, Josbert J Keller, Ed J Kuijper
Since 2013, several stool banks have been developed following publications reporting on clinical success of "Fecal Microbiota Transplantation" (FMT) for recurrent Clostridium difficile infections (CDI). However, protocols for donor screening, fecal suspension preparation and transfer of the fecal suspension differ between various countries and institutions. Moreover, no European consensus exists regarding the legislative aspects of the fecal suspension product. Internationally standardized recommendations about the above mentioned aspects have not yet been established...
May 18, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28513974/fecal-microbiota-transplantation-outcomes-in-immunocompetent-and-immunocompromised-patients-a-single-center-experience
#3
Sally Alrabaa, Ripal Jariwala, Kristen Zeitler, Jose Montero
BACKGROUND: Clostridium difficile infection (CDI) is a major infectious disease focus for which fecal microbiota transplantation (FMT) has been used with success in various patient populations. METHODS: We conducted a retrospective study of FMT in immunocompetent and immunocompromised patients to review outcomes at our center, with a focus on identifying risk factors for FMT failure in solid organ transplant (SOT) patients. FMT was conducted using universal banked frozen stool via naso-duodenal tube in patients with recurrent CDI of 3 or more episodes per our institutional protocol...
May 17, 2017: Transplant Infectious Disease: An Official Journal of the Transplantation Society
https://www.readbyqxmd.com/read/28471618/a-retrospective-comparison-of-fecal-microbial-transplantation-methods-for-recurrent-clostridium-difficile-infection
#4
Nathaniel A Cohen, Dan M Livovsky, Shir Yaakobovitch, Merav Ben Yehoyada, Ronen Ben Ami, Amos Adler, Hanan Guzner-Gur, Eran Goldin, Moshe E Santo, Zamir Halpern, Kalman Paz, Nitsan Maharshak
BACKGROUND: Antibiotic treatment of Clostridium difficile infection (CDI) has a high failure rate. Fecal microbiota transplantation (FMT) has proven very effective in treating these recurrences. OBJECTIVES: To determine which method of fecal microbiota transplantation (upper or lower gastrointestinal) and which type of donor (a relative or unrelated) is superior. METHODS: This is a retrospective analysis of treatment protocols and outcomes in 22 patients with refractory or recurrent CDI who underwent FMT at two Israeli facilities...
October 2016: Israel Medical Association Journal: IMAJ
https://www.readbyqxmd.com/read/28412936/-1-h-nmr-analysis-of-feces-new-possibilities-in-the-helminthes-infections-research
#5
Sarantos Kostidis, Daria Kokova, Natalia Dementeva, Irina V Saltykova, Hye Kyong Kim, Young Hae Choi, Oleg A Mayboroda
BACKGROUND: Analysis of the stool samples is an essential part of routine diagnostics of the helminthes infections. However, the standard methods such Kato and Kato-Katz utilize only a fraction of the information available. Here we present a method based on the nuclear magnetic resonance spectroscopy (NMR) which could be auxiliary to the standard procedures by evaluating the complex metabolic profiles (or phenotypes) of the samples. METHOD: The samples were collected over the period of June-July 2015, frozen at -20 °C at the site of collection and transferred within four hours for the permanent storage at -80 °C...
April 17, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28359329/comparison-of-stool-versus-rectal-swab-samples-and-storage-conditions-on-bacterial-community-profiles
#6
Christine M Bassis, Nicholas M Moore, Karen Lolans, Anna M Seekatz, Robert A Weinstein, Vincent B Young, Mary K Hayden
BACKGROUND: Sample collection for gut microbiota analysis from in-patients can be challenging. Collection method and storage conditions are potential sources of variability. In this study, we compared the bacterial microbiota from stool stored under different conditions, as well as stool and swab samples, to assess differences due to sample storage conditions and collection method. METHODS: Using bacterial 16S rRNA gene sequence analysis, we compared the microbiota profiles of stool samples stored and collected under various conditions...
March 31, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28290266/improved-diagnosis-of-trichuris-trichiura-by-using-a-bead-beating-procedure-on-ethanol-preserved-stool-samples-prior-to-dna-isolation-and-the-performance-of-multiplex-real-time-pcr-for-intestinal-parasites
#7
Maria M M Kaisar, Eric A T Brienen, Yenny Djuardi, Erliyani Sartono, Maria Yazdanbakhsh, Jaco J Verweij, Taniawati Supali, Lisette VAN Lieshout
For the majority of intestinal parasites, real-time PCR-based diagnosis outperforms microscopy. However, the data for Trichuris trichiura have been less convincing and most comparative studies have been performed in populations with low prevalence. This study aims to improve detection of T. trichuria DNA in human stool by evaluating four sample preparation methods. Faecal samples (n = 60) were collected at Flores island, Indonesia and examined by microscopy. Aliquots were taken and a bead-beating procedure was used both on directly frozen stool and on material preserved with 96% ethanol...
March 14, 2017: Parasitology
https://www.readbyqxmd.com/read/28260999/gut-microbiota-analysis-results-are-highly-dependent-on-the-16s-rrna-gene-target-region-whereas-the-impact-of-dna-extraction-is-minor
#8
Anniina Rintala, Sami Pietilä, Eveliina Munukka, Erkki Eerola, Juha-Pekka Pursiheimo, Asta Laiho, Satu Pekkala, Pentti Huovinen
Next-generation sequencing (NGS) is currently the method of choice for analyzing gut microbiota composition. As gut microbiota composition is a potential future target for clinical diagnostics, it is of utmost importance to enhance and optimize the NGS analysis procedures. Here, we have analyzed the impact of DNA extraction and selected 16S rDNA primers on the gut microbiota NGS results. Bacterial DNA from frozen stool specimens was extracted with 5 commercially available DNA extraction kits. Special attention was paid to the semiautomated DNA extraction methods that could expedite the analysis procedure, thus being especially suitable for clinical settings...
April 2017: Journal of Biomolecular Techniques: JBT
https://www.readbyqxmd.com/read/28220514/randomised-clinical-trial-faecal-microbiota-transplantation-for-recurrent-clostridum-difficile-infection-fresh-or-frozen-or-lyophilised-microbiota-from-a-small-pool-of-healthy-donors-delivered-by-colonoscopy
#9
Z D Jiang, N J Ajami, J F Petrosino, G Jun, C L Hanis, M Shah, L Hochman, V Ankoma-Sey, A W DuPont, M C Wong, A Alexander, S Ke, H L DuPont
BACKGROUND: Faecal microbiota transplantation (FMT) has become routine in managing recurrent C. difficile infection (CDI) refractory to antibiotics. AIM: To compare clinical response and improvements in colonic microbiota diversity in subjects with recurrent CDI using different donor product. METHODS: Seventy-two subjects with ≥3 bouts of CDI were randomised in a double-blind study to receive fresh, frozen or lyophilised FMT product via colonoscopy from 50 g of stool per treatment from eight healthy donors...
February 21, 2017: Alimentary Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/28121648/changes-in-the-gut-microbiota-after-early-administration-of-oral-synbiotics-to-young-infants-in-india
#10
Dinesh S Chandel, Maria E Perez-Munoz, Fang Yu, Robert Boissy, Radhanath Satpathy, Pravas R Misra, Nidhi Sharma, Rama Chaudhry, Sailajanandan Parida, Daniel A Peterson, Ira H Gewolb, Pinaki Panigrahi
OBJECTIVES: We examined the changes in the developing gut microbiota of Indian infants enrolled in a colonization study of an oral synbiotic (L. plantarum and fructo-oligosaccharides) preparation. METHODS: Frozen stool samples were available from a previously published clinical study of the synbiotic preparation administered daily for seven days to full term Indian infants delivered by C-section. 16S rRNA gene sequencing of fecal bacterial community-DNA was done in 11 infants sampled at Day-7 and Day-60 of life...
January 25, 2017: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/28103236/impact-of-cd4-t-cell-responses-on-clinical-outcome-following-oral-administration-of-wild-type-enterotoxigenic-escherichia-coli-in-humans
#11
Monica A McArthur, Wilbur H Chen, Laurence Magder, Myron M Levine, Marcelo B Sztein
Enterotoxigenic Escherichia coli (ETEC) is a non-invasive enteric pathogen of considerable public health importance, being one of the most common attributable causes of diarrheal illness in infants and young children in developing countries and the most common cause of traveler's diarrhea. To enhance study-to-study consistency of our experimental challenge model of ETEC in volunteers, and to allow concomitant multi-site trials to evaluate anti-ETEC immunoprophylactic products, hundreds of vials, each containing a standardized inoculum of virulent wild-type (wt) ETEC strain H10407 (serotype O78:H11 expressing colonization factor antigen I and heat-labile and heat-stable enterotoxins), were prepared under current Good Manufacturing Practices (cGMP) and frozen...
January 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28000190/-short-term-efficacy-on-fecal-microbiota-transplantation-combined-with-soluble-dietary-fiber-and-probiotics-in-the-treatment-of-slow-transit-constipation
#12
Xiaolong Ge, Chao Ding, Jianfeng Gong, Hongliang Tian, Yao Wei, Qiyi Chen, Lili Gu, Ning Li
OBJECTIVE: To evaluate the efficacy and safety of fecal microbiota transplantation (FMT) combined with soluble dietary fiber and probiotics for slow transit constipation(STC). METHODS: Twenty-three patients with STC from Jinling Hospital, Medical School of Nanjing University were prospectively enrolled between April 2015 and January 2016. STC patients received FMT combined with soluble dietary fiber and probiotics. Fresh stool(100 g) was immediately mixed in a blender with 500 ml of 0...
December 25, 2016: Zhonghua Wei Chang Wai Ke za Zhi, Chinese Journal of Gastrointestinal Surgery
https://www.readbyqxmd.com/read/27927919/clinical-evaluation-and-cost-analysis-of-great-basin-shiga-toxin-direct-molecular-assay-for-detection-of-shiga-toxin-producing-escherichia-coli-in-diarrheal-stool-specimens
#13
Matthew L Faron, Nathan A Ledeboer, Jessica Connolly, Paul A Granato, Brenda R Alkins, Jennifer Dien Bard, Judy A Daly, Stephen Young, Blake W Buchan
The Shiga Toxin Direct molecular assay (ST Direct) relies on nucleic acid amplification and solid array-based amplicon detection to identify Shiga toxin-producing Escherichia coli (STEC) in preserved stool specimens. Genes encoding Shiga toxin (stx1 and stx2), as well as the E. coli serotype O:157-specific marker rfbE, are simultaneously detected within 2 h. ST Direct was evaluated using 1,084 prospectively collected preserved stool specimens across five clinical centers. An additional 55 retrospectively collected, frozen specimens were included to increase the number of positive specimens evaluated...
February 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27812352/impact-of-different-fecal-processing-methods-on-assessments-of-bacterial-diversity-in-the-human-intestine
#14
Yu-Hsin Hsieh, Courtney M Peterson, Anne Raggio, Michael J Keenan, Roy J Martin, Eric Ravussin, Maria L Marco
The intestinal microbiota are integral to understanding the relationships between nutrition and health. Therefore, fecal sampling and processing protocols for metagenomic surveys should be sufficiently robust, accurate, and reliable to identify the microorganisms present. We investigated the use of different fecal preparation methods on the bacterial community structures identified in human stools. Complete stools were collected from six healthy individuals and processed according to the following methods: (i) randomly sampled fresh stool, (ii) fresh stool homogenized in a blender for 2 min, (iii) randomly sampled frozen stool, and (iv) frozen stool homogenized in a blender for 2 min, or (v) homogenized in a pneumatic mixer for either 10, 20, or 30 min...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27811291/fungal-microbiota-profile-in-newly-diagnosed-treatment-na%C3%A3-ve-children-with-crohn-s-disease
#15
Mohammad El Mouzan, Feng Wang, Mohammad Al Mofarreh, Rajita Menon, Ahmad Al Barrag, Kirill S Korolev, Ahmad Al Sarkhy, Mona Al Asmi, Yassin Hamed, Anjum Saeed, Scot E Dowd, Asaad Assiri, Harland Winter
Background and Aims: Although increasing evidence suggests a role for fungi in inflammatory bowel disease [IBD], data are scarce and mostly from adults. Our aim was to define the characteristics of fungal microbiota in newly diagnosed treatment-naïve children with Crohn's disease [CD]. Methods: The children referred for colonoscopy were prospectively enrolled in the study at King Khalid University Hospital, King Saud University, and Al Mofarreh Polyclinics in Riyadh...
May 1, 2017: Journal of Crohn's & Colitis
https://www.readbyqxmd.com/read/27793749/fresh-vs-frozen-samples-and-ambient-temperature-have-little-effect-on-detection-of-colorectal-cancer-or-adenomas-by-a-fecal-immunochemical-test-in-a-colorectal-cancer-screening-cohort-in-germany
#16
Hongda Chen, Simone Werner, Hermann Brenner
BACKGROUND & AIMS: Fecal immunochemical tests (FITs) are used in colorectal cancer (CRC) screening. We compared detection of CRCs and colorectal neoplasms by FITs using fresh samples (collected into buffer-filled tubes) vs frozen samples, and we assessed the effects of seasonal variations in ambient temperature on test performance. METHODS: We performed a prospective study of 3466 individuals (50% male; mean age, 62 years) undergoing screening colonoscopies at 20 gastroenterology practices in southern Germany from November 2008 through September 2014...
October 26, 2016: Clinical Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/27558918/a-robust-ambient-temperature-collection-and-stabilization-strategy-enabling-worldwide-functional-studies-of-the-human-microbiome
#17
Ericka L Anderson, Weizhong Li, Niels Klitgord, Sarah K Highlander, Mark Dayrit, Victor Seguritan, Shibu Yooseph, William Biggs, J Craig Venter, Karen E Nelson, Marcus B Jones
As reports on possible associations between microbes and the host increase in number, more meaningful interpretations of this information require an ability to compare data sets across studies. This is dependent upon standardization of workflows to ensure comparability both within and between studies. Here we propose the standard use of an alternate collection and stabilization method that would facilitate such comparisons. The DNA Genotek OMNIgene∙Gut Stool Microbiome Kit was compared to the currently accepted community standard of freezing to store human stool samples prior to whole genome sequencing (WGS) for microbiome studies...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27544433/the-impact-of-handling-and-storage-of-human-fecal-material-on-bacterial-activity
#18
Eleni Karatza, Maria Vertzoni, Uwe Muenster, Christos Reppas
Fecal material prepared from human stools is frequently used for the assessment of bacterial degradation of active pharmaceutical ingredients as relevant data are useful for evaluating the potential for colonic drug delivery. The impact of handling and storage of human fecal material on bacterial activity was assessed by evaluating the degradation characteristics of metronidazole and olsalazine. Multiple freeze (-70°C)-thaw cycles should be avoided. Incubation of frozen material for about 2 h in the anaerobic workstation ensures regeneration of the highest possible bacterial activity...
November 2016: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/27534405/physical-pre-treatment-improves-efficient-dna-extraction-and-qpcr-sensitivity-from-clostridium-difficile-spores-in-faecal-swine-specimens
#19
Łukasz Grześkowiak, Jürgen Zentek, Wilfried Vahjen
A considerable fraction of the faecal microbiota is spore-forming. Molecular quantification of bacteria may be underestimated if preceded with nucleic acid extraction without special treatment to extract recalcitrant bacterial spores. The objective of this study was to improve the DNA extraction regarding the presence of Clostridium difficile spores in faecal swine specimens. Sow faeces were inoculated with spores of C. difficile (10(6) CFU), frozen at - 30 °C overnight and subjected to DNA extraction. As a preceding step to a standard DNA extraction method (QIAamp DNA stool Mini kit), different physical treatments such as microwave oven heating and repeated bead-beating techniques and a combination of both were applied and compared with each other by means of qPCR...
November 2016: Current Microbiology
https://www.readbyqxmd.com/read/27530517/is-dried-stool-spots-on-filter-paper-method-dssfp-more-sensitive-and-effective-for-detecting-blastocystis-spp-and-their-subtypes-by-pcr-and-sequencing
#20
Ayse Seyer, Djursun Karasartova, Emrah Ruh, Ayse Semra Güreser, Turgut Imir, Aysegul Taylan-Ozkan
PCR and DNA sequencing are currently the diagnostic methods of choice for detection of Blastocystis spp. and their suptypes. Fresh or frozen stool samples have disadvantages in terms of several aspects such as transportation, storage, and existence of PCR inhibitors. Filter paper technology may provide a solution to these issues. The aim of the present study was to detect Blastocystis spp. and their subtypes by employing two different preservation methods: conventional frozen stool (FS) and dried stool spots on filter paper (DSSFP)...
December 2016: Parasitology Research
keyword
keyword
83789
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"