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https://www.readbyqxmd.com/read/28205456/histochemical-study-of-encephalitozoon-cuniculi-spores-in-the-kidneys-of-naturally-infected-new-zealand-rabbits
#1
Luis E Rodríguez-Tovar, Alejandra Villarreal-Marroquín, Alicia M Nevárez-Garza, Uziel Castillo-Velázquez, Heidi G Rodríguez-Ramírez, Magda C Navarro-Soto, Juan J Zárate-Ramos, Gustavo Hernández-Vidal, Armando Trejo-Chávez
Encephalitozoon cuniculi is an important microsporidian pathogen that is considered an emergent, zoonotic, and opportunistic. It infects both domestic and laboratory rabbits, generating severe chronic interstitial and granulomatous nephritis with fibrosis and granulomatous encephalitis. Encephalitozoonosis is diagnosed in paraffin-embedded sections by examining the spores in the host tissues. The spores are difficult to observe when the samples are stained with hematoxylin and eosin (H&E), particularly when there is an inflammatory reaction and tissue damage...
February 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28199765/survey-for-zoonotic-microsporidian-pathogens-in-wild-living-urban-rooks-corvus-frugilegus
#2
Agnieszka Perec-Matysiak, Maria Wesołowska, Kinga Leśniańska, Katarzyna Buńkowska-Gawlik, Joanna Hildebrand, Marta Kicia
Microsporidia are opportunistic pathogens in nature infecting all animal phyla. There is a potential risk of microsporidian spores transmission from urban rooks inhabiting some metropolitan cities to people through casual interactions. The aim of this study was to identify microsporidia species in the droppings of rooks in Wroclaw, Poland. A total of 15 collective sets of droppings were examined using nested-PCR method. Amplification of ITS rRNA gene revealed the presence of Enterocytozoon bieneusi D, Peru 6 and Encephalitozoon hellem 1A genotypes...
February 15, 2017: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/28199389/correction-a-large-collection-of-novel-nematode-infecting-microsporidia-and-their-diverse-interactions-with-caenorhabditis-elegans-and-other-related-nematodes
#3
(no author information available yet)
[This corrects the article DOI: 10.1371/journal.ppat.1006093.].
February 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28171733/microsporidiosis-in-four-tortoises-testudo-hermanni-boettgeri
#4
M Eydner, J Donhauser, A Beineke, P Guenther, S Blahak
Microsporidia are obligate intracellular, spore-forming fungi. A wide range of vertebrate and invertebrate hosts can be infected; however, cases of infected turtles or tortoises have not yet been described. This is the first description of 4 cases in tortoises ( Testudo spp), which showed general signs of illness as well as intestinal and respiratory signs until death occurred or they were euthanized. Granulomatous and necrotizing inflammation was visible in the lung and liver. Fungal organisms were present in heart blood, lung, liver, and intestine...
January 1, 2017: Veterinary Pathology
https://www.readbyqxmd.com/read/28152065/microsporidia-infection-impacts-the-host-cell-s-cycle-and-reduces-host-cell-apoptosis
#5
Raquel Martín-Hernández, Mariano Higes, Soledad Sagastume, Ángeles Juarranz, Joyce Dias-Almeida, Giles E Budge, Aránzazu Meana, Neil Boonham
Intracellular parasites can alter the cellular machinery of host cells to create a safe haven for their survival. In this regard, microsporidia are obligate intracellular fungal parasites with extremely reduced genomes and hence, they are strongly dependent on their host for energy and resources. To date, there are few studies into host cell manipulation by microsporidia, most of which have focused on morphological aspects. The microsporidia Nosema apis and Nosema ceranae are worldwide parasites of honey bees, infecting their ventricular epithelial cells...
2017: PloS One
https://www.readbyqxmd.com/read/28115231/molecular-detection-and-species-identification-of-enterocytozoon-bieneusi-isolated-from-immunocompetent-orang-asli-in-malaysia
#6
Azah Ashikin, Hesham M Al-Mekhlafi, Norhayati Moktar, Tengku Shahrul Anuar
Most studies of opportunistic infections focus on immunocompromised patients. However, there is a lack of information on microsporidiosis in healthy people (immunocompetent) worldwide. This study aimed to detect and identify microsporidia species in immunocompetent Orang Asli living in Pahang, Malaysia. Orang Asli is a collective term for a group of indigenous people that usually reside in the interior regions of Peninsular Malaysia. They comprise about 0.7% of the total population in Malaysia and 76% of them lived below the poverty line i...
April 2017: Parasitology International
https://www.readbyqxmd.com/read/28111712/morphological-characterization-and-hsp70-igs-based-phylogenetic-analysis-of-two-microsporidian-parasites-isolated-from-antheraea-pernyi
#7
Yong Wang, Yutao Ru, Wei Liu, Deyi Wang, Jinglin Zhou, Yiren Jiang, Shenglin Shi, Li Qin
Two microsporidian isolates were extracted from single infected egg-laying tussah silk moth (Antheraea pernyi) in Liaoning Province, China. The microsporidia were subsequently grown in silk moth larvae, isolated, and subjected to morphological characterization (by light and transmission electron microscopy) and phylogenetic analysis (based on conserved genes). One type of spore was long-axis-oval in shape, measuring 4.71 × 1.95 μm, and the other type was short-axis-oval, measuring 3.64 × 2.17 μm. These dimensions were markedly different from those reported in the spores of the common microsporidia infecting A...
January 22, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28096853/microsporidial-spores-in-fecal-samples-of-some-domesticated-animals-living-in-giza-egypt
#8
Ahmad Z Al-Herrawy, Mahmoud A Gad
BACKGROUND: The aim of the present work was to investigate the prevalence and species of intestinal microsporidiosis among animals in Giza, Egypt. METHODS: A total of 869 animal fecal samples were collected from domesticated animals (dogs, cats, rabbits, cattle, buffaloes, sheep, goats, donkeys and pigs) living in Giza, Egypt. Spores of microsporidia were concentrated from collected samples by centrifugation and finally stained with modified trichrome (MT) stain to detect microsporidial spores...
April 2016: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28067236/identification-of-microsporidia-host-exposed-proteins-reveals-a-repertoire-of-rapidly-evolving-proteins
#9
Aaron W Reinke, Keir M Balla, Eric J Bennett, Emily R Troemel
Pathogens use a variety of secreted and surface proteins to interact with and manipulate their hosts, but a systematic approach for identifying such proteins has been lacking. To identify these 'host-exposed' proteins, we used spatially restricted enzymatic tagging followed by mass spectrometry analysis of Caenorhabditis elegans infected with two species of Nematocida microsporidia. We identified 82 microsporidia proteins inside of intestinal cells, including several pathogen proteins in the nucleus. These microsporidia proteins are enriched in targeting signals, are rapidly evolving and belong to large Nematocida-specific gene families...
January 9, 2017: Nature Communications
https://www.readbyqxmd.com/read/28031263/interaction-between-swp9-and-ptps-of-the-microsporidian-nosema-bombycis-and-swp9-as-a-scaffolding-protein-contributes-to-the-polar-tube-tethering-to-spore-wall
#10
Donglin Yang, Lixia Pan, Pai Peng, Xiaoqun Dang, Chunfeng Li, Tian Li, Mengxian Long, Jie Chen, Yujiao Wu, Huihui Du, Bo Luo, Yue Song, Rui Tian, Jie Luo, Zeyang Zhou, Guoqing Pan
All the members of microsporidia possess a unique, highly specialized invasion mechanism that involves the polar tube and spore wall. The interaction between spore wall proteins (SWPs) and polar tube proteins (PTPs) in the formation, arrangement, orderly orientation and function of the polar tube and spore wall remain to be determined. This study was undertaken to examine the protein interactions of the Nosema bombycis SWP7, SWP9 and PTPs. Co-immunoprecipitation, LC-MS/MS and yeast two hybrid data demonstrated that NbSWP9, but not NbSWP7, interacts with NbPTP1 and NbPTP2...
December 28, 2016: Infection and Immunity
https://www.readbyqxmd.com/read/28030354/phylogenetic-analysis-of-genetically-distinct-enterocytozoon-bieneusi-infecting-renal-transplant-recipients
#11
Sonali Khanduja, Ujjala Ghoshal, Uday C Ghoshal
Enterocytozoon bieneusi (E. bieneusi), infecting renal transplant (RT) recipients may be transmitted anthroponotically or zoonotically. Accordingly, we aimed to, a) evaluate genotypes of E. bieneusi ínfecting RT recipients, and b) infer phylogenetic interpretation on transmission of different genotypes among infected hosts. Stool samples of 22 RT recipients infected with microsporidia (identified using modified trichrome staining) were subjected to species identification. All E. bieneusi positive samples were subjected to genotyping...
March 1, 2017: Acta Parasitologica
https://www.readbyqxmd.com/read/27995672/biofilms-for-monitoring-presence-of-microsporidia-in-environmental-water
#12
Joseph A Moss, Richard A Snyder
The development of molecular methodologies for targeting pathogens such as the Microsporidia has greatly improved our monitoring capabilities and initiatives. This study analyzed samples collected from 5 locations in Pensacola, Florida, USA for the presence of Microsporidian pathogens. To circumvent various impediments associated with water collection and filtration, we utilized biofilms as sentinels for detection of Microsporidia. We implemented membrane-dissolution and sample purification in a single confined step followed by real-time PCR to confirm pathogen presence...
December 20, 2016: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/27993617/parahepatospora-carcini-n-gen-n-sp-a-parasite-of-invasive-carcinus-maenas-with-intermediate-features-of-sporogony-between-the-enterocytozoon-clade-and-other-microsporidia
#13
Jamie Bojko, Fraser Clark, David Bass, Alison M Dunn, Sarah Stewart-Clark, Paul D Stebbing, Grant D Stentiford
Parahepatospora carcini n. gen. n. sp., is a novel microsporidian parasite discovered infecting the cytoplasm of epithelial cells of the hepatopancreas of a single Carcinus maenas specimen. The crab was sampled from within its invasive range in Atlantic Canada (Nova Scotia). Histopathology and transmission electron microscopy were used to show the development of the parasite within a simple interfacial membrane, culminating in the formation of unikaryotic spores with 5-6 turns of an isofilar polar filament...
December 18, 2016: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/27989634/heterologous-expression-of-paranosema-antonospora-locustae-hexokinase-in-lepidopteran-sf9-cells-is-followed-by-accumulation-of-the-microsporidian-protein-in-insect-cell-nuclei
#14
Sergey A Timofeev, Igor V Senderskiy, Alexander A Tsarev, Yuri S Tokarev, Viacheslav V Dolgikh
Paranosema (Nosema, Antonospora) locustae is the only microsporidium produced as a commercial product for biological control. Molecular mechanisms of the effects of this pathogen and other invertebrate microsporidia on host cells remain uncharacterized. Previously, we immunolocalized P. locustae hexokinase in nuclei of Locusta migratoria infected adipocytes. Here, the microsporidian protein was expressed in the yeast Pichia pastoris and in lepidopteran Sf9 cells. During heterologous expression, P. locustae hexokinase was accumulated in the nuclei of insect cells but not in yeast cell nuclei...
December 16, 2016: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/27989633/the-microsporidium-nosema-disstriae-thomson-1959-fine-structure-and-phylogenetic-position-within-the-n-bombycis-clade
#15
George Kyei-Poku, Yuliya Y Sokolova
A microsporidium Nosema disstriae (Thomson) is a parasite of the forest tent caterpillar Malacasoma disstria (Lepidoptera: Lasiocampidae), a notable defoliator of deciduous trees in North America. The goal of this paper was to demonstrate the ultrastructure of N. disstriae and to determine the position of this microsporidium within the N. bombycis clade (NBC) using comparative morphology and multiple molecular phylogenetic markers: RPB1, LSU-, ITS- and SSU-rDNA. As a part of this goal, the revision of the described members of the NBC has been performed...
December 15, 2016: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/27987526/molecular-phylogeny-of-paraphelidium-letcheri-sp-nov-aphelida-opisthosporidia
#16
Sergey A Karpov, Guifré Torruella, David Moreira, Maria A Mamkaeva, Purificación López-García
Aphelids remain poorly known parasitoids of algae and have recently raised considerable interest due to their phylogenetic position at the base of Holomycota. Together with Cryptomycota (Rozellosporidia) and Microsporidia, they have been recently re-classified as the Opisthosporidia, which constitutes the sister group to the fungi within the Holomycota. Molecular environmental studies have revealed a huge diversity of aphelids, but only four genera have been described: Aphelidium, Amoeboaphelidium, Paraphelidium, and Pseudaphelidium...
December 17, 2016: Journal of Eukaryotic Microbiology
https://www.readbyqxmd.com/read/27987055/description-and-phylogeny-of-a-new-microsporidium-from-the-elm-leaf-beetle-xanthogaleruca-luteola-muller-1766-coleoptera-chrysomelidae
#17
Çağrı Bekircan, Ufuk Bülbül, Halil I Güler, James J Becnel
This study describes a new genus and species of microsporidia which is a pathogen of the elm leaf beetle, Xanthogaleruca luteola Muller, 1776 (Coleoptera: Chrysomelidae). The beetles were collected from Istanbul in Turkey. All developmental stages are uninucleate and in direct contact with the host cell cytoplasm. Giemsa-stained mature spores are oval in shape and measured 3.40 ± 0.37 μm in length and 1.63 ± 0.20 μm in width. These uninucleate spores have an isofilar polar filament with 11 turns...
February 2017: Parasitology Research
https://www.readbyqxmd.com/read/27942022/a-large-collection-of-novel-nematode-infecting-microsporidia-and-their-diverse-interactions-with-caenorhabditis-elegans-and-other-related-nematodes
#18
Gaotian Zhang, Martin Sachse, Marie-Christine Prevost, Robert J Luallen, Emily R Troemel, Marie-Anne Félix
Microsporidia are fungi-related intracellular pathogens that may infect virtually all animals, but are poorly understood. The nematode Caenorhabditis elegans has recently become a model host for studying microsporidia through the identification of its natural microsporidian pathogen Nematocida parisii. However, it was unclear how widespread and diverse microsporidia infections are in C. elegans or other related nematodes in the wild. Here we describe the isolation and culture of 47 nematodes with microsporidian infections...
December 2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27934806/molecular-appraisal-of-intestinal-parasitic-infection-in-transplant-recipients
#19
Pooja Yadav, Shehla Khalil, Bijay Ranjan Mirdha
BACKGROUND & OBJECTIVES: Diarrhoea is the main clinical manifestation caused by intestinal parasitic infections in patients, with special reference to transplant recipients who require careful consideration to reduce morbidity and mortality. Further, molecular characterization of some important parasites is necessary to delineate the different modes of transmission to consider appropriate management strategies. We undertook this study to investigate the intestinal parasitic infections in transplant recipients with or without diarrhoea, and the genotypes of the isolated parasites were also determined...
August 2016: Indian Journal of Medical Research
https://www.readbyqxmd.com/read/27855212/microsporidia-why-make-nucleotides-if-you-can-steal-them
#20
REVIEW
Paul Dean, Robert P Hirt, T Martin Embley
Microsporidia are strict obligate intracellular parasites that infect a wide range of eukaryotes including humans and economically important fish and insects. Surviving and flourishing inside another eukaryotic cell is a very specialised lifestyle that requires evolutionary innovation. Genome sequence analyses show that microsporidia have lost most of the genes needed for making primary metabolites, such as amino acids and nucleotides, and also that they have only a limited capacity for making adenosine triphosphate (ATP)...
November 2016: PLoS Pathogens
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