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Gap junctions tumor antigen

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https://www.readbyqxmd.com/read/26187405/differential-effects-of-polyoma-virus-middle-tumor-antigen-mutants-upon-gap-junctional-intercellular-communication
#1
Mulu Geletu, Stephanie Guy, Samantha Greer, Leda Raptis
Gap junctions are channels that connect the cytoplasm of adjacent cells. Oncogenes such as the middle Tumor antigen of polyoma virus (mT) are known to suppress gap junctional, intercellular communication (GJIC). mT associates with and is tyrosine-phosphorylated by cSrc family members. Specific mT phosphotyrosines provide docking sites for the phosphotyrosine binding domain of Shc (mT-tyr250) or the SH2 domain of the regulatory subunit of the phosphatidylinositol-3 kinase (PI3k, mT-tyr315). Binding results in the activation of their downstream signaling cascades, Ras/Raf/Erk and PI3 kinase/Akt, respectively, both of which are needed for full neoplastic transformation...
August 15, 2015: Experimental Cell Research
https://www.readbyqxmd.com/read/25529637/connexin-expression-in-human-acute-myeloid-leukemia-cells-identification-of-patient-subsets-based-on-protein-and-global-gene-expression-profiles
#2
Håkon Reikvam, Anita Ryningen, Lars Rune Sæterdal, Ina Nepstad, Brynjar Foss, Øystein Bruserud
Bone marrow stromal cells support both normal and malignant hematopoiesis. Τhis support is mediated through the local cytokine network and by direct cell‑cell interactions mediated via adhesion molecules and the formation of gap junctions by connexins. Previous studies on connexins in human acute myeloid leukemia (AML) have mainly focused on the investigation of leukemia cell lines. In the present study, we therefore investigated the expression of various connexins at the protein (i.e., cell surface expression) and mRNA level in primary human AML cells...
March 2015: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/25447529/cd44-variant-but-not-standard-cd44-isoforms-mediate-disassembly-of-endothelial-ve-cadherin-junction-on-metastatic-melanoma-cells
#3
Pu Zhang, Changliang Fu, Huiyuan Bai, Erqun Song, Yang Song
Loss of endothelial adherens junctions is involved in tumor metastasis. Here, we demonstrate that, in the metastatic Lu1205 melanoma cells, expression of the CD44 variant CD44v8-v10 induced junction disassembly and vascular endothelial (VE)-cadherin phosphorylation at Y658 and Y731. Short interfering RNA (siRNA)-mediated CD44 knockdown or sialic acid cleavage reversed these effects. Moreover, microspheres coated with recombinant CD44v8-v10 promoted endothelial junction disruption. Overexpression of CD44v8-v10 but not of standard CD44 (CD44s) promoted gap formation in the non-metastatic WM35 melanoma cells, whereas CD44 knockdown or neuraminidase treatment dramatically diminished melanoma transendothelial migration...
December 20, 2014: FEBS Letters
https://www.readbyqxmd.com/read/25204183/-study-on-anti-tumor-and-anti-metastasis-mechanism-of-alcohol-extracts-from-pharbitidis-semen-against-lewis-lung-cancer
#4
Jia-Huan Li, Gang-Jun Du, Wei-Jie Liu, Ying-Hui Liu, Bei Zhao, Hong Li
OBJECTIVE: To observe the effect of alcohol extracts from Pharbitidis Semen on the proliferation and metastasis of Lewis lung cancer, and study its anti-tumor mechanism. METHOD: In vitro, MTT assay and scratch assay were adopted to detect the effect of alcohol extracts from Pharbitidis Semen on the proliferation and metastasis of Lewis lung cancer cells. The cell autophagy was detected by the acridine orange staining. The gap-junction intercellular communication (GJIC) was investigated by the fluorescent yellow transfer...
March 2014: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/24593809/icam-2-regulates-vascular-permeability-and-n-cadherin-localization-through-ezrin-radixin-moesin-erm-proteins-and-rac-1-signalling
#5
Valerie Amsellem, Nicola H Dryden, Roberta Martinelli, Felicity Gavins, Lourdes Osuna Almagro, Graeme M Birdsey, Dorian O Haskard, Justin C Mason, Patric Turowski, Anna M Randi
BACKGROUND: Endothelial junctions control functions such as permeability, angiogenesis and contact inhibition. VE-Cadherin (VECad) is essential for the maintenance of intercellular contacts. In confluent endothelial monolayers, N-Cadherin (NCad) is mostly expressed on the apical and basal membrane, but in the absence of VECad it localizes at junctions. Both cadherins are required for vascular development. The intercellular adhesion molecule (ICAM)-2, also localized at endothelial junctions, is involved in leukocyte recruitment and angiogenesis...
March 4, 2014: Cell Communication and Signaling: CCS
https://www.readbyqxmd.com/read/24439748/exposing-endothelial-cells-to-tumor-necrosis-factor-%C3%AE-and-peripheral-blood-mononuclear-cells-damage-endothelial-integrity-via-interleukin-1%C3%A3-by-degradation-of-vascular-endothelial-cadherin
#6
Ann L B Seynhaeve, Joost A P Rens, Debby Schipper, Alexander M M Eggermont, Timo L M Ten Hagen
BACKGROUND AND PURPOSE: We demonstrated previously that the administration of tumor necrosis factor alpha (TNF-α) for the treatment of solid tumors enhanced the response to chemotherapy by augmenting intratumoral drug accumulation. TNF-α changes the integrity of the endothelial cell monolayer in combination with interferon gamma (IFN-γ), which is further enhanced by the addition of peripheral blood mononuclear cells (PBMCs). The improved effect of PBMCs was mostly induced by the endogenous production of interleukin-1beta (IL-1ß) after TNF-α stimulation...
March 2014: Surgery
https://www.readbyqxmd.com/read/24287273/h-pylori-encoded-caga-disrupts-tight-junctions-and-induces-invasiveness-of-ags-gastric-carcinoma-cells-via-cdx2-dependent-targeting-of-claudin-2
#7
Xin Song, Hui-Xin Chen, Xiao-Yan Wang, Xi-Yun Deng, Yin-Xue Xi, Qing He, Tie-Li Peng, Jie Chen, Wei Chen, Benjamin Chun-Yu Wong, Min-Hu Chen
Helicobacter pylori encoded CagA is presently the only known virulence factor that is injected into gastric epithelial cells where it destroys apical junctional complexes and induces dedifferentiation of gastric epithelial cells, leading to H. pylori-related gastric carcinogensis. However, little is known about the molecular mechanisms by which CagA mediates these changes. Caudal-related homeobox 2 (Cdx2) is an intestine-specific transcription factor highly expressed in multistage tissues of dysplasia and cancer...
November 2013: Cellular Immunology
https://www.readbyqxmd.com/read/24046352/a-mouse-model-of-pathological-small-intestinal-epithelial-cell-apoptosis-and-shedding-induced-by-systemic-administration-of-lipopolysaccharide
#8
Jonathan M Williams, Carrie A Duckworth, Alastair J M Watson, Mark R Frey, Jennifer C Miguel, Michael D Burkitt, Robert Sutton, Kevin R Hughes, Lindsay J Hall, Jorge H Caamaño, Barry J Campbell, D Mark Pritchard
The gut barrier, composed of a single layer of intestinal epithelial cells (IECs) held together by tight junctions, prevents the entrance of harmful microorganisms, antigens and toxins from the gut lumen into the blood. Small intestinal homeostasis is normally maintained by the rate of shedding of senescent enterocytes from the villus tip exactly matching the rate of generation of new cells in the crypt. However, in various localized and systemic inflammatory conditions, intestinal homeostasis can be disturbed as a result of increased IEC shedding...
November 2013: Disease Models & Mechanisms
https://www.readbyqxmd.com/read/23744108/regulation-of-gap-junctions-in-melanoma-and-their-impact-on-melan-a-mart-1-specific-cd8%C3%A2-%C2%BA-t-lymphocyte-emergence
#9
Houssem Benlalam, Thibault Carré, Abdelali Jalil, Zaeem Noman, Bernard Caillou, Philippe Vielh, Andrés Tittarelli, Caroline Robert, Salem Chouaib
UNLABELLED: Gap junctions (GJs) enable intercellular communication between adjacent cells through channels of connexins. Using a three-dimensional construct, we previously showed that endothelial and tumor cells formed GJs, allowing melanoma-specific T lymphocytes to recognize and kill melanoma-derived endothelial cells. We demonstrate here on histological sections of melanoma biopsies that GJ formation occurs in vivo between tumor and endothelial cells and between T lymphocytes and target cells...
October 2013: Journal of Molecular Medicine: Official Organ of the "Gesellschaft Deutscher Naturforscher und Ärzte"
https://www.readbyqxmd.com/read/23374644/the-potential-prognostic-value-of-connexin-26-and-46-expression-in-neoadjuvant-treated-breast-cancer
#10
Ivett Teleki, Tibor Krenacs, Marcell A Szasz, Janina Kulka, Barna Wichmann, Cornelia Leo, Barbel Papassotiropoulos, Cosima Riemenschnitter, Holger Moch, Zsuzsanna Varga
BACKGROUND: Several classification systems are available to assess pathological response to neoadjuvant chemotherapy in breast cancer, but reliable biomarkers to predict the efficiency of primary systemic therapy (PST) are still missing. Deregulation of gap junction channel forming connexins (Cx) has been implicated in carcinogenesis and tumour progression through loss of cell cycle control. In this study we correlated Cx expression and cell proliferation with disease survival and pathological response to neoadjuvant chemotherapy in breast cancers using existing classification systems...
February 2, 2013: BMC Cancer
https://www.readbyqxmd.com/read/23351380/connexin32-inhibits-gastric-carcinogenesis-through-cell-cycle-arrest-and-altered-expression-of-p21cip1-and-p27kip1
#11
Hyang Jee, Su-Hyung Lee, Jun-Won Park, Bo-Ram Lee, Ki-Taek Nam, Dae-Yong Kim
Gap junctions and their structural proteins, connexins (Cxs), have been implicated in carcinogenesis. To explore the involvement of Cx32 in gastric carcinogenesis, immunochemical analysis of Cx32 and proliferation marker Ki67 using tissue-microarrayed human gastric cancer and normal tissues was performed. In addition, after Cx32 overexpression in the human gastric cancer cell line AGS, cell proliferation, cell cycle analyses, and p21(Cip1) and p27(Kip1) expression levels were examined by bromodeoxyuridine assay, flow cytometry, real-time RT-PCR, and western blotting...
January 2013: BMB Reports
https://www.readbyqxmd.com/read/23205268/delineation-of-breast-cancer-cell-hierarchy-identifies-the-subset-responsible-for-dormancy
#12
Shyam A Patel, Shakti H Ramkissoon, Margarette Bryan, Lillian F Pliner, Gabriela Dontu, Prem S Patel, Sohrab Amiri, Sharon R Pine, Pranela Rameshwar
The bone marrow (BM) is a major organ of breast cancer (BC) dormancy and a common source of BC resurgence. Gap junctional intercellular communication (GJIC) between BC cells (BCCs) and BM stroma facilitates dormancy. This study reports on a hierarchy of BCCs with the most immature subset (Oct4(hi)/CD44(hi/med)/CD24(-/+)) demonstrating chemoresistance, dormancy, and stem cell properties: self-renewal, serial passaging ability, cycling quiescence, long doubling time, asymmetric division, high metastatic and invasive capability...
2012: Scientific Reports
https://www.readbyqxmd.com/read/22648953/p120-catenin-prevents-neutrophil-transmigration-independently-of-rhoa-inhibition-by-impairing-src-dependent-ve-cadherin-phosphorylation
#13
Pilar Alcaide, Roberta Martinelli, Gail Newton, Marcie R Williams, Alejandro Adam, Peter A Vincent, Francis W Luscinskas
Leukocyte transendothelial migration (TEM) is regulated by several signaling pathways including Src family kinases (SFK) and the small RhoGTPases. Previous studies have shown that vascular endothelial-cadherin (VE-cad) forms a complex with β-,γ-, and p120-catenins and this complex disassociates to form a transient gap during leukocyte TEM. Additionally, p120-catenin (p120-1A) overexpression in human umbilical vein endothelial cells (HUVEC) stabilizes VE-cad surface expression, prevents tyrosine phosphorylation of VE-cad, and inhibits leukocyte TEM...
August 15, 2012: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/22431192/impact-of-vegf-on-astrocytes-analysis-of-gap-junctional-intercellular-communication-proliferation-and-motility
#14
Ricarda Wuestefeld, Jingchen Chen, Karl Meller, Beate Brand-Saberi, Carsten Theiss
The purpose of the present study was to investigate the effects of vascular endothelial growth factor (VEGF) on gap junctional intercellular communication (GJIC), cell proliferation, and cell dynamics in primary astrocytes. VEGF is known as a dimeric polypeptide that potentially binds to two receptors, VEGFR-1 and VEGFR-2, however many effects are mediated by VEGFR-2, for example, actin polymerization, forced cell migration, angiogenesis, and cell proliferation. Recently it has been shown that in case of hypoxia, ischemia or injury VEGF is upregulated to stimulate angiogenesis and cell proliferation...
May 2012: Glia
https://www.readbyqxmd.com/read/21811634/tumor-associated-macrophages-tams-form-an-interconnected-cellular-supportive-network-in-anaplastic-thyroid-carcinoma
#15
Bernard Caillou, Monique Talbot, Urbain Weyemi, Catherine Pioche-Durieu, Abir Al Ghuzlan, Jean Michel Bidart, Salem Chouaib, Martin Schlumberger, Corinne Dupuy
BACKGROUND: A relationship between the increased density of tumor-associated macrophages (TAMs) and decreased survival was recently reported in thyroid cancer patients. Among these tumors, anaplastic thyroid cancer (ATC) is one of the most aggressive solid tumors in humans. TAMs (type M2) have been recognized as promoting tumor growth. The purpose of our study was to analyze with immunohistochemistry the presence of TAMs in a series of 27 ATC. METHODOLOGY/PRINCIPAL FINDINGS: Several macrophages markers such as NADPH oxidase complex NOX2-p22phox, CD163 and CD 68 were used...
2011: PloS One
https://www.readbyqxmd.com/read/21607924/lack-of-prognostic-significance-of-connexin-43-labeling-in-a-series-of-46-gastrointestinal-stromal-tumors
#16
Marko Boban, Neven Ljubicic, Marko Nikolic, Davor Tomas, Mario Zovak, Miroslav Bekavac-Beslin, Borislav Belev, Jasna Radic, Milan Milosevic
BACKGROUND: Gastrointestinal stromal tumors (GISTs) are mesenchymal tumors with variable malignant potential. Connexin-43 (Cx43) is the commonest gap-junction protein and has been frequently investigated in oncology. Our aim was to establish the immunohistochemical expression of Cx43 in relation to GIST location, size, Ki67 index, tumor grade and follow-up.? MATERIALS AND METHODS: The study included postoperative samples of 46 patients treated for GIST in the 1999-2010 time frame...
April 2011: International Journal of Biological Markers
https://www.readbyqxmd.com/read/21563192/emmprin-regulates-cytoskeleton-reorganization-and-cell-adhesion-in-prostate-cancer
#17
Haining Zhu, Jun Zhao, Beibei Zhu, Joanne Collazo, Jozsef Gal, Ping Shi, Li Liu, Anna-Lena Ström, Xiaoning Lu, Richard O McCann, Michal Toborek, Natasha Kyprianou
BACKGROUND: Proteins on cell surface play important roles during cancer progression and metastasis via their ability to mediate cell-to-cell interactions and navigate the communication between cells and the microenvironment. METHODS: In this study a targeted proteomic analysis was conducted to identify the differential expression of cell surface proteins in human benign (BPH-1) versus malignant (LNCaP and PC-3) prostate epithelial cells. We identified EMMPRIN (extracellular matrix metalloproteinase inducer) as a key candidate and shRNA functional approaches were subsequently applied to determine the role of EMMPRIN in prostate cancer cell adhesion, migration, invasion as well as cytoskeleton organization...
January 2012: Prostate
https://www.readbyqxmd.com/read/21173791/prognostic-value-of-connexin43-expression-in-patients-with-clinically-localized-prostate-cancer
#18
G Benko, B Spajić, A Demirović, G Stimac, B Kru Sbreve Lin, D Tomas
Connexins (Cxs) are a family of transmembrane proteins that build cell-to-cell channels in gap junctions. Gap junctions composed of Cxs have an essential role in intercellular communication, adhesion and cell differentiation. Several studies investigated the role of connexin43 (Cx43) in different carcinomas; however, none investigated its prognostic role in prostate cancer. Cx43 expression and relationship with established prognostic features were assessed in a cohort of 102 patients treated with radical prostatectomy for clinically localized prostate adenocarcinoma...
March 2011: Prostate Cancer and Prostatic Diseases
https://www.readbyqxmd.com/read/21082351/altered-expression-and-localization-of-connexin32-in-human-and-murine-gastric-carcinogenesis
#19
Hyang Jee, Ki Taek Nam, Hyo-Jung Kwon, Sang-Uk Han, Dae-Yong Kim
BACKGROUND: Intercellular communication via gap junctions, composed of protein subunits called connexins (Cxs), plays a key role in controlling cell growth, differentiation and carcinogenesis. Impaired gap junctional intercellular communication has been reported in various cancers and diseases. AIMS: We investigated Cx32 expression patterns and semiquantitatively assessed Cx32 expression in cancers and preneoplastic lesions. To determine if cell proliferation is correlated with Cx32 expression, we evaluated Ki67 expression in a gastric cancer mouse model...
May 2011: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/20702856/bacteria-induced-gap-junctions-in-tumors-favor-antigen-cross-presentation-and-antitumor-immunity
#20
Fabiana Saccheri, Chiara Pozzi, Francesca Avogadri, Sara Barozzi, Mario Faretta, Paola Fusi, Maria Rescigno
Antigen-presenting dendritic cells (DCs) trigger the activation of cytotoxic CD8 T cells that target and eliminate cells with the antigen on their surface. Although DCs usually pick up and process antigens themselves, they can also receive peptide antigens from other cells via gap junctions. We demonstrate here that infection with Salmonella can induce, in both human and murine melanoma cells, the up-regulation of connexin 43 (Cx43), a ubiquitous protein that forms gap junctions and that is normally lost during melanoma progression...
August 11, 2010: Science Translational Medicine
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