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https://www.readbyqxmd.com/read/29350905/ampulexins-a-new-family-of-peptides-in-venom-of-the-emerald-jewel-wasp-ampulex-compressa
#1
Eugene Moore, Ryan Arvidson, Christopher Banks, Jean Urenda, Elizabeth Duong, Haroun Mohammad, Michael E Adams
The parasitoid wasp Ampulex compressa injects venom directly into the brain and subesophageal ganglion of the cockroach Periplaneta americana, inducing a seven to ten day lethargy termed hypokinesia. Hypokinesia presents as a significant reduction in both escape response and spontaneous walking. We examined aminergic and peptidergic components of milked venom with HPLC and MALDI-TOF mass spectrometry. HPLC coupled with electrochemical detection confirmed presence of dopamine in milked venom, while mass spectrometry revealed that the venom gland and venom sac have distinct peptide profiles, with milked venom predominantly composed of venom sac peptides...
January 19, 2018: Biochemistry
https://www.readbyqxmd.com/read/29349932/isolation-and-characterization-of-lectin-with-antibacterial-antibiofilm-and-antiproliferative-activities-from-acinetobacter-baumannii-of-environmental-origin
#2
Abdullah A Alyousef, Abdulaziz Alqasim, Mustafa Sawsan Aloahd
AIMS: Lectin is a non-immunogenic glycoprotein, have been extracted majority from the plant source primarily leguminoasea. Its ability to precisely recognize and bind to the complex cell bound structure to enable it playing diverse roles. In this study, we obligate to define new sources of lectins since the production of lectins is very expensive. Therefore, we performed a study with the goal to isolate and characterize lectin from bacteria of plant origin and screen its ability as an antibacterial, antibiofilm and antiproliferative agent...
January 19, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29348860/silencing-ror1-and-ror2-inhibits-invasion-and-adhesion-in-an-organotypic-model-of-ovarian-cancer-metastasis
#3
Claire Henry, Neville Hacker, Caroline Ford
OBJECTIVE: Elevated expression of the ROR1 and ROR2 Wnt receptors has been noted in both the tumour and stromal compartments of ovarian cancer patient tissue samples. In vitro studies have suggested these receptors play a role in ovarian cancer metastasis. However, these previous studies have utilised simple 2D in vitro models to investigate cancer cell growth and migration, which does not allow investigation of stromal involvement in Wnt driven metastasis. AIM: To investigate targeting ROR1 and ROR2 using a primary co-culture 3D model of epithelial ovarian cancer dissemination to the omentum...
December 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/29347632/dynamics-of-a-thermally-driven-film-climbing-the-outside-of-a-vertical-cylinder
#4
Linda B Smolka
The dynamics of a film climbing the outside of a vertical cylinder under the competing effects of a thermally driven surface tension gradient and gravity is examined through numerical simulations of a thin-film model for the film height. The model, including boundary conditions, depends on three parameters, the scaled cylinder radius R[over ̂], the upstream film height h_{∞}, and the downstream precursor film thickness b, and reduces to the model for Marangoni driven film climbing a vertical plate in the limit R[over ̂]→∞...
October 2017: Physical Review. E
https://www.readbyqxmd.com/read/29347118/linear-stability-of-layered-two-phase-flows-through-parallel-soft-gel-coated-walls
#5
B Dinesh, S Pushpavanam
The linear stability of layered two-phase Poiseuille flows through soft-gel-coated parallel walls is studied in this work. The focus is on determining the effect of the elastohydrodynamic coupling between the fluids and the soft-gel layers on the different instabilities observed in flows between parallel plates. The fluids are assumed Newtonian and incompressible, while the soft gels are modeled as linear viscoelastic solids. A long-wave asymptotic analysis is used to obtain an analytical expression for the growth rate of the disturbances...
July 2017: Physical Review. E
https://www.readbyqxmd.com/read/29344080/effects-of-huang-bai-phellodendri-cortex-on-bone-growth-and-pubertal-development-in-adolescent-female-rats
#6
Sun Haeng Lee, Hyun Jeong Lee, Sung Hyun Lee, Young-Sik Kim, Donghun Lee, Jiu Chun, Jin Yong Lee, Hocheol Kim, Gyu Tae Chang
Background: To evaluate the effects of Huang Bai (Phellodendron amurense) on growth and maturation in adolescent female rats. Methods: Female Sprague-Dawley rats (28 days old; n = 72) were divided into six daily treatment groups: control (distilled water), Huang Bai (100 and 300 mg/kg), recombinant human GH (rhGH; 20 μg/kg), estradiol (1 μg/kg), and triptorelin (100 μg). Body weight, food intake, and vaginal opening were measured daily from postnatal day (PND) 28 to PND 43...
2018: Chinese Medicine
https://www.readbyqxmd.com/read/29343835/a-non-zircon-hf-isotope-record-in-archean-black-shales-from-the-pilbara-craton-confirms-changing-crustal-dynamics-ca-3-ga-ago
#7
Yona Nebel-Jacobsen, Oliver Nebel, Martin Wille, Peter A Cawood
Plate tectonics and associated subduction are unique to the Earth. Studies of Archean rocks show significant changes in composition and structural style around 3.0 to 2.5 Ga that are related to changing tectonic regime, possibly associated with the onset of subduction. Whole rock Hf isotope systematics of black shales from the Australian Pilbara craton, selected to exclude detrital zircon components, are employed to evaluate the evolution of the Archean crust. This approach avoids limitations of Hf-in-zircon analyses, which only provide input from rocks of sufficient Zr-concentration, and therefore usually represent domains that already underwent a degree of differentiation...
January 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29343450/paediatric-physeal-sparing-posterior-cruciate-ligament-pcl-reconstruction-with-parental-donation-allograft-rationale-and-operative-approach
#8
R S Khakha, S K Yasen, A J Wilson
BACKGROUND: Paediatric PCL injuries are rare but constitute a significant management challenge. We describe a novel approach to the surgical management of an 11-year-old boy who presented with persisting symptomatic instability following 18months of failed conservative therapy. METHODS: PCL reconstruction was performed using a physeal sparing, all-inside technique under fluoroscopic control. This avoids the potential for iatrogenic growth injury. A parentally donated hamstrings allograft was used to ensure adequate graft size, and reinforced using a non-elastic two millimetre braided suture...
January 14, 2018: Knee
https://www.readbyqxmd.com/read/29342080/comparisons-of-different-models-on-dynamic-recrystallization-of-plate-during-asymmetrical-shear-rolling
#9
Tao Zhang, Lei Li, Shi-Hong Lu, Hai Gong, Yun-Xin Wu
Asymmetrical shear rolling with velocity asymmetry and geometry asymmetry is beneficial to enlarge deformation and refine grain size at the center of the thick plate compared to conventional symmetrical rolling. Dynamic recrystallization (DRX) plays a vital role in grain refinement during hot deformation. Finite element models (FEM) coupled with microstructure evolution models and cellular automata models (CA) are established to study the microstructure evolution of plate during asymmetrical shear rolling. The results show that a larger DRX fraction and a smaller average grain size can be obtained at the lower layer of the plate...
January 17, 2018: Materials
https://www.readbyqxmd.com/read/29340862/effect-of-brewery-wastewater-obtained-from-different-phases-of-treatment-plant-on-seed-germination-of-chickpea-cicer-arietinum-maize-zea-mays-and-pigeon-pea-cajanus-cajan
#10
Rupa Salian, Suhas Wani, Ramamohan Reddy, Mukund Patil
Brewing industry releases large quantities of wastewater after product generation. Brewery wastewater contains organic compounds which are biodegradable in nature. These biodegradable wastes can be recycled and reused and hence considered as suitable products for agriculture. But before using wastewater for agriculture, it is better to evaluate the phytotoxic effects of wastewater on crops. Hence, the main objective of this study is to evaluate the effects of brewery effluent on seed germination and growth parameters of selected crop species like chickpea (Cicer arietinum), maize (Zea mays), and pigeon pea (Cajanus cajan)...
January 17, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29339848/habitual-patellar-dislocation-in-children-results-of-surgical-treatment-by-modified-four-in-one-technique
#11
Ravi Mittal, Avtar Singh Balawat, Vikrant Manhas, Amit Roy, Navin Kumar Singh
Background: Habitual patellar dislocation is a rare condition affecting children. The growth plates are open in children and any surgical intervention should take that in to consideration. We are describing a modified four in one technique for habitual patellar dislocation, which is a soft tissue procedure without the use of any implant. Method: In this study we included 6 children (4 females and 2 males) with open growth plates, which were diagnosed with habitual patellar dislocation...
November 2017: Journal of Clinical Orthopaedics and Trauma
https://www.readbyqxmd.com/read/29339306/morphological-changes-of-bacterial-cells-upon-exposure-of-silver-silver-chloride-nanoparticles-synthesized-using-agrimonia-pilosa
#12
Maheshkumar Prakash Patil, Yong Bae Seo, Gun-Do Kim
Facile, eco-friendly synthesis of metal nanoparticles has been proposed as a cost effective method. In the present study, we propose the facile synthesis of silver-silver chloride (Ag-AgCl) nanoparticles (NPs) using the medicinally important Agrimonia pilosa plant extract without addition of capping or stabilizing agents. The Ag-AgCl NPs synthesis was observed at 40 °C after 10 min incubation; the synthesis of Ag-AgCl NPs was indicated by color change and confirmed by UV-vis spectroscopic peak at 454 nm...
January 12, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29338819/in-vitro-efficacy-of-continuous-mild-high-temperature-on-the-biofilm-formation-of-aspergillus-niger
#13
Rong Zeng, Jian Bo Tong, Yu Zhen Liu, Qing Chen, Tong Lin, Min Li, Gui Xia Lü
Objective To investigate whether continuous mild high temperature (increased temperature without causing significant damage to host cells) can inhibit the biofilm formation of Aspergillus niger (A.niger) and its vitality.Methods A.niger biofilms were formed on a coverslip in 24-well tissue culture plate and were checked at the time points 4,8,10,16,24,48 and 72 hours.Confocal laser scanning microscopy (CLSM) was used to image and quantify A.niger biofilm formation under three different continuous mild high temperatures at 37℃,39℃,and 41℃...
December 20, 2017: Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae
https://www.readbyqxmd.com/read/29338725/herbal-formula-ygjdsj-inhibits-anchorage-independent-growth-and-induces-anoikis-in-hepatocellular-carcinoma-bel-7402-cells
#14
Bing Hu, Tong Zhang, Hong-Mei An, Jia-Lu Zheng, Xia Yan, Xiao-Wei Huang
BACKGROUND: Based on clinical medications and related studies, we established a Yang-Gan Jie-Du Sang-Jie (YGJDSJ) herbal formula for hepatocarcinoma treatment. In present study, we evaluated the anti-cancer potential of YGJDSJ on suspension-grown human hepatocellular carcinoma Bel-7402 cells. METHODS: Bel-7402 cells were cultured in poly(2-hydroxyethyl methacrylate) (poly-HEMA) coated plates and treated with YGJDSJ. Anchorage-independent cell growth was detected by cell Counting Kit-8 (CCK-8) assay and soft agar colony formation assay...
January 16, 2018: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/29337618/vegetative-valvular-endocarditis-and-hepatitis-associated-with-helcococcus-ovis-in-a-7-year-old-white-leghorn-rooster
#15
Manuela Crispo, Simone Stoute, Thaiza Savaris, Arthur Bickford, Tiffany Santoro, C Gabriel Sentíes-Cué
Helcococcus ovis is a slow-growing, pyridoxal-dependent, Gram-positive coccus belonging to the Peptostreptococcaceae family. Bacteria belonging to the genus Helcococcus are considered normal inhabitants of keratinized epithelium in humans; however, several reports support their role as pathogens in humans and several animal species. This case report describes the identification of H. ovis in a white leghorn rooster with valvular vegetative endocarditis and hepatitis. In February 2017 one dead, 7-yr-old, white leghorn rooster was submitted to the California Animal Health and Food Safety Turlock laboratory for diagnostic testing...
December 2017: Avian Diseases
https://www.readbyqxmd.com/read/29336609/fibroblast-growth-factor-23-fgf23-gene-polymorphisms-are-associated-with-essential-hypertension-risk-and-blood-pressure-levels-in-chinese-han-population
#16
Peng Cai, Yan Peng, Li Li, Wei Chu, Xukai Wang
In this case-control study, 246 EH patients and 157 healthy controls were selected from Chinese Han population to explore the associations between the fibroblast growth factor 23 (FGF23) gene polymorphisms and essential hypertension (EH).The SequenomMassarray system was used for the genotyping of three FGF23 gene Tag single-nucleotide polymorphisms, namely rs7955866, rs13312756, and rs3812822. The primers were designed by Assay Designer 3.1 software, and then the samples were added to a 384-well plate for the polymerase chain reaction amplification, shrimp alkaline phosphatase reaction, and desalting after extension...
January 16, 2018: Clinical and Experimental Hypertension: CHE
https://www.readbyqxmd.com/read/29334476/biological-control-of-botrytis-cinerea-interactions-with-native-vineyard-yeasts-from-washington-state
#17
Xuefei Wang, Dean A Glawe, Elizabeth K Kramer, David M Weller, Patricia Ann Okubara
Native yeasts are of increasing interest to researchers, grape growers, and vintners because of their potential for biocontrol activity, and their contributions to the aroma, flavor and mouthfeel qualities of wines. To assess biocontrol activity, we tested 11 yeasts from Washington vineyards, representing isolates of Candida saitoana, Curvibasidium pallidicorallinum, Metschnikowia chrysoperlae, Metschnikowia pulcherrima, Meyerozyma guilliermondii, Saccharomyces cerevisiae and Wickerhamomyces anomalus, for ability to colonize 'Thompson Seedless' grape berries, inhibit the growth of Botrytis cinerea in vitro, and suppress disease symptoms on isolated berries...
January 15, 2018: Phytopathology
https://www.readbyqxmd.com/read/29331470/the-effect-of-different-precooling-rates-and-cold-storage-on-milk-microbiological-quality-and-composition
#18
Lizandra F Paludetti, Alan L Kelly, Bernadette O'Brien, Kieran Jordan, David Gleeson
The objective of this study was to measure the effect of different milk cooling rates, before entering the bulk tank, on the microbiological load and composition of the milk, as well as on energy usage. Three milk precooling treatments were applied before milk entered 3 identical bulk milk tanks: no plate cooler (NP), single-stage plate cooler (SP), and double-stage plate cooler (DP). These precooling treatments cooled the milk to 32.0 ± 1.4°C, 17.0 ± 2.8°C, and 6.0 ± 1.1°C, respectively. Milk was added to the bulk tank twice daily for 72 h, and the tank refrigeration temperature was set at 3°C...
January 10, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29331414/inhibition-of-fasn-and-er%C3%AE-signalling-during-hyperglycaemia-induced-matrix-specific-emt-promotes-breast-cancer-cell-invasion-via-a-caveolin-1-dependent-mechanism
#19
H A Zielinska, J M P Holly, A Bahl, C M Perks
Since disturbed metabolic conditions such as obesity and diabetes can be critical determinants of breast cancer progression and therapeutic failure, we aimed to determine the mechanism responsible for their pro-oncogenic effects. Using non-invasive, epithelial-like ERα-positive MCF-7 and T47D human breast cancer cells we found that hyperglycaemia induced epithelial to mesenchymal transition (EMT), a key programme responsible for the development of metastatic disease. This was demonstrated by loss of the epithelial marker E-cadherin together with increases in mesenchymal markers such as vimentin, fibronectin and the transcription factor SLUG, together with an enhancement of cell growth and invasion...
January 10, 2018: Cancer Letters
https://www.readbyqxmd.com/read/29329921/assessment-of-test-methods-for-evaluating-effectiveness-of-cleaning-flexible-endoscopes
#20
Rebecca E Washburn, Jennifer J Pietsch
BACKGROUND: Strict adherence to each step of reprocessing is imperative to removing potentially infectious agents. Multiple methods for verifying proper reprocessing exist; however, each presents challenges and limitations, and best practice within the industry has not been established. Our goal was to evaluate endoscope cleaning verification tests with particular interest in the evaluation of the manual cleaning step. The results of the cleaning verification tests were compared with microbial culturing to see if a positive cleaning verification test would be predictive of microbial growth...
January 9, 2018: American Journal of Infection Control
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