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https://www.readbyqxmd.com/read/28727416/in-situ-electrochemical-sensing-and-real-time-monitoring-live-cells-based-on-freestanding-nanohybrid-paper-electrode-assembled-from-3d-functionalized-graphene-framework
#1
Yan Zhang, Jian Xiao, Qi-Ying Lv, Lu Wang, Xulin Dong, Muhammad Asif, Jinghua Ren, Wenshan He, Yimin Sun, Fei Xiao, Shuai Wang
In this work, we develop a new type of freestanding nanohybrid paper electrode assembled from 3D ionic liquid (IL) functionalized graphene framework (GF) decorated by gold nanoflowers (AuNFs), and explore its practical application in in-situ electrochemical sensing of live breast cell samples by real-time tracking biomarker H2O2 released from cells. The AuNFs modified IL functionalized GF (AuNFs/IL-GF) was synthesized via a facile and efficient dopamine assisted one-pot self-assembly strategy. The as-obtained nanohybrid assembly exhibits a typical 3D hierarchical porous structure, where the highly active electrocatalyst AuNFs are well dispersed on IL-GF scaffold...
July 20, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28726916/a-novel-3-d-bio-microfluidic-system-mimicking-in-vivo-heterogeneous-tumour-microstructures-reveals-complex-tumour-stroma-interactions
#2
Qihui Fan, Ruchuan Liu, Yang Jiao, Chunxiu Tian, James D Farrell, Wenwen Diao, Xiaochen Wang, Fengrong Zhang, Wei Yuan, Haibo Han, Jinfeng Chen, Yue Yang, Xixiang Zhang, Fangfu Ye, Ming Li, Zhongcan Ouyang, Liyu Liu
A 3-D microfluidic system consisting of microchamber arrays embedded in a collagen hydrogel with tuneable biochemical gradients that mimics the tumour microenvironment of mammary glands was constructed for the investigation on the interactions between invasive breast cancer cells and stromal cells. The hollow microchambers in collagen provide a very similar 3-D environment to that in vivo that regulates collective cellular dynamics and behaviour, while the microfluidic channels surrounding the collagen microchamber arrays allow one to impose complex concentration gradients of specific biological molecules or drugs...
July 20, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28726720/tgf-%C3%AE-dependent-growth-arrest-and-cell-migration-in-benign-and-malignant-breast-epithelial-cells-are-antagonistically-controlled-by-rac1-and-rac1b
#3
Catharina Melzer, Juliane von der Ohe, Ralf Hass, Hendrik Ungefroren
Despite improvements in diagnosis and treatment, breast cancer is still the most common cancer type among non-smoking females. TGF-β can inhibit breast cancer development by inducing cell cycle arrest in both, cancer cells and, as part of a senescence program in normal human mammary epithelial cells (HMEC). Moreover, TGF-β also drives cell migration and invasion, in part through the small GTPases Rac1 and Rac1b. Depletion of Rac1b or Rac1 and Rac1b in MDA-MB-231 or MDA-MB-435s breast cancer cells by RNA interference enhanced or suppressed, respectively, TGF-β1-induced migration/invasion...
July 20, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28724364/tamoxifen-reverses-epithelial-mesenchymal-transition-by-demethylating-mir-200c-in-triple-negative-breast-cancer-cells
#4
Qian Wang, Yu Cheng, Yan Wang, Yibo Fan, Ce Li, Ye Zhang, Yiding Wang, Qian Dong, Yanju Ma, Yue-E Teng, Xiujuan Qu, Yunpeng Liu
BACKGROUND: Although the efficacy of tamoxifen (TAM) for breast cancer has been attributed to inducing cell cycle arrest and apoptosis by inhibiting estrogen receptor (ER) signaling, recent evidence indicates that TAM also possesses ER-independent antitumor activity through an unclear mechanism. The present study investigated the anti-tumor mechanism of TAM on mesenchymal triple-negative breast cancer (TNBC). METHODS: The inhibitory effect of TAM on tumor migration and metastasis was analyzed by transwell chamber in vitro and by murine xenograft model in vivo...
July 19, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28724327/gemcitabine-hydrochloride-loaded-liposomes-and-nanoparticles-comparison-of-encapsulation-efficiency-drug-release-particle-size-and-cytotoxicity
#5
Tahir Emre Yalcin, Sibel Ilbasmis-Tamer, Burçin Ibisoglu, Aysun Özdemir, Mustafa Ark, Sevgi Takka
The aim of this study is to formulate and compare the physicochemical properties of negatively-charged liposomes and poly-lactide-co-glycolide (PLGA) nanoparticles loaded with gemcitabine hydrochloride. The influence of the formulation variables on the liposome and nanoparticle properties on particle size, zeta potential, encapsulation efficiency and drug release were evaluated. Although the PEGylated nanoparticles and PEGylated liposomes were of the same size (∼200 nm), the encapsulation efficiency was 1...
July 20, 2017: Pharmaceutical Development and Technology
https://www.readbyqxmd.com/read/28723662/acetyl-lupeolic-acid-inhibits-akt-signaling-and-induces-apoptosis-in-chemoresistant-prostate-cancer-cells-in-vitro-and-in-vivo
#6
Claudia Schmidt, Cornelia Loos, Lu Jin, Michael Schmiech, Christoph Q Schmidt, Menna El Gaafary, Tatiana Syrovets, Thomas Simmet
The triterpenoid acetyl-lupeolic acid (ac-LA) isolated from the oleogum resin of Boswellia carterii reduced the viability of a panel of cancer cell lines more efficiently than lupeol. There was no detectable intracellular conversion of ac-LA to lupeol and vice versa. In contrast to docetaxel, ac-LA did not induce selection of treatment-resistant cancer cells. By various parameters including DNA fragmentation, ac-LA was shown to induce apoptosis in androgen-independent PC-3 cells, whereas in MDA-MB-231 breast cancer cells, ac-LA led to cell accumulation in the G2/M phase of the cell cycle, but not to apoptosis...
July 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/28723623/daurinol-blocks-breast-and-lung-cancer-metastasis-and-development-by-inhibition-of-focal-adhesion-kinase-fak
#7
Jong K Woo, Hyun J Jung, Ji-Youn Park, Ju-Hee Kang, Byung I Lee, DongYun Shin, Chu W Nho, Soo-Young Cho, Je K Seong, Seung H Oh
FAK overexpression has been reported in diverse primary and metastatic tumor tissues, supporting its pro-tumorigenic and pro-metastatic roles. Therefore, we have developed a neo-treatment strategy using daurinol to effectively treat cancer metastasis. Daurinol blocked cancer cell migration and invasion in vitro and exhibited anti-metastatic activity in an experimental metastasis model of breast and lung cancer. Daurinol selectively inhibited phosphorylation of FAK at Tyr925, Tyr576/577, and Tyr397 sites in a dose- and time-dependent manner...
July 4, 2017: Oncotarget
https://www.readbyqxmd.com/read/28723132/single-cell-mechanical-characteristics-analyzed-by-multiconstriction-microfluidic-channels
#8
Xiang Ren, Parham Ghassemi, Hesam Babahosseini, Jeannine S Strobl, Masoud Agah
A microfluidic device composed of variable numbers of multiconstriction channels is reported in this paper to differentiate a human breast cancer cell line, MDA-MB-231, and a nontumorigenic human breast cell line, MCF-10A. Differences between their mechanical properties were assessed by comparing the effect of single or multiple relaxations on their velocity profiles which is a novel measure of their deformation ability. Videos of the cells were recorded via a microscope using a smartphone, and imported to a tracking software to gain the position information on the cells...
February 24, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28720470/molecular-docking-prediction-and-in-vitro-studies-elucidate-anti-cancer-activity-of-phytoestrogens
#9
Shreelekha Dutta, Prashant S Kharkar, Niteshkumar U Sahu, Aparna Khanna
AIM: The study is aimed at evaluating the chemosensitization and apoptotic effect of aglycone rich extracts of dietary phytoestrogens (derived from soybean and flaxseed) on estrogen receptor positive, MCF-7 and estrogen receptor negative, MDA-MB-231 cells. The extracts show potent activity on both the cell lines, hence, in silico studies have been carried out to find the possible reason for their activity. MAIN METHODS: MTT assay was carried to assess chemosensitization effect and activated caspase-3/7 activity was studied using flow-cytometry and western blotting...
July 15, 2017: Life Sciences
https://www.readbyqxmd.com/read/28720062/hes1-overexpression-promotes-cell-proliferation-and-invasion-in-breast-cancer-and-predicts-poor-survival
#10
Xiaoying Li, Yu Cao, Mu Li, Feng Jin
HES1 is a transcriptional repressor involved in cell differentiation and proliferation, but the expression pattern and biological roles of HES1 in breast cancer have not been examined. In this study, we examined HES1 expression in breast cancer tissues using immunohistochemistry and western blot analyses and investigated HES1 function using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and Matrigel invasion assays. Significant relationships were observed between HES1 upregulation and advanced tumour-node-metastasis stage (p = 0...
July 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28719146/natural-compounds-in-cancer-prevention-effects-of-coffee-extracts-and-their-main-polyphenolic-component-5-cqa-on-oncogenic-ras-proteins
#11
Alessandro Palmioli, Carlotta Cairamelli, Renata Tisi, Michela Spinelli, Gaia De Sanctis, Elena Sacco, Cristina Airoldi
Recent epidemiological studies demonstrate that consumption of healthy foods, especially rich in polyphenols content, might reduce the incidence of cancer and degenerative diseases. In particular, chlorogenic acids (CGAs) occur ubiquitously in food, representing the most abundant polyphenols in the human diet. A number of CGA beneficial biological effects, including anti-inflammatory activity, anti-carcinogenic activity and protection against neurodegenerative diseases have been reported. However, the molecular mechanisms at the basis of these biological activities have not yet been investigated in depth...
July 18, 2017: Chemistry, An Asian Journal
https://www.readbyqxmd.com/read/28719045/mical2-promotes-breast-cancer-cell-migration-by-maintaining-egfr-stability-and-egfr-p38-signaling-activation
#12
Yueyuan Wang, Wenjie Deng, Yujie Zhang, Shixiu Sun, Shuo Zhao, Yan Chen, Xuyang Zhao, Lei Liu, Jun Du
AIM: MICAL2, a cytoskeleton dynamics regulator, is identified associated with survival and metastasis of several types of cancers recently. The present study was designed to investigate the role of MICAL2 in breast cancer cell migration as well as its underlying mechanisms. METHODS: The relationship between MICAL2 and EGF/EGFR signaling was analyzed by using gene overexpression and knockdown techniques. Cell migration was measured by wound healing assays. Activation of EGF/EGFR singaling pathways were evaluated by immunofluorescence, qPCR, Western blotting and zymography techniques...
July 18, 2017: Acta Physiologica
https://www.readbyqxmd.com/read/28718672/synthesis-anticancer-and-apoptosis-inducing-activities-of-quinazoline-isatin-conjugates-epidermal-growth-factor-receptor-tyrosine-kinase-assay-and-molecular-docking-studies
#13
Adel S El-Azab, Abdullah Al-Dhfyan, Alaa A-M Abdel-Aziz, Laila A Abou-Zeid, Hamad M Alkahtani, Abdulrahman M Al-Obaid, Manal A Al-Gendy
A new series of quinazolinone compounds 16-34 incorporating isatin moieties was synthesized. The antitumor efficacy of the compounds against MDA-MB-231, a breast cancer cell line, and LOVO, a colon cancer cell line, was assessed. Compounds 20, 21, 22, 23, 25, 27, 28, 29, 30, 31, 32, 33, and 34 displayed potent antitumor activity against MDA-MB-231 and LOVO cells (IC50: 10.38-38.67 μM and 9.91-15.77 μM, respectively); the comparative IC50 values for 5-fluorouracil and erlotinib in these cells lines were 70...
December 2017: Journal of Enzyme Inhibition and Medicinal Chemistry
https://www.readbyqxmd.com/read/28718278/matrix-stiffness-differentially-regulates-cellular-uptake-behavior-of-nanoparticles-in-two-breast-cancer-cell-lines
#14
Yu Wang, Tao Gong, Zhirong Zhang, Yao Fu
Matrix stiffness regulates cell behavior in various biological contexts. In breast tumors, the deposition of extracellular matrix (ECM) correlates with increasing matrix stiffness and poor survival. Nanoparticulate carriers represent a promising therapeutic vehicle for disease diagnosis and efficient anticancer drug delivery. However, how matrix stiffness influences cellular uptake of nanoparticle remains largely unexplored. Here, we selected photopolymerized polyacrylamide gels with varying stiffness as model substrates, and studied the impact of matrix stiffness on cell morphology, nanoparticle uptake efficiency in two representative breast cancer cell lines with varying invasiveness, i...
July 18, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28717943/a-pyrrole-based-natural-small-molecule-mitigates-hsp90-expression-in-mda-mb-231-cells-and-inhibits-tumor-angiogenesis-in-mice-by-inactivating-hsf-1
#15
K C Rashmi, H S Atreya, M Harsha Raj, Bharathi P Salimath, H S Aparna
Heat shock proteins (HSPs), molecular chaperones, are crucial for the cancer cells to facilitate proper functioning of various oncoproteins involved in cell survival, proliferation, migration, and tumor angiogenesis. Tumor cells are said to be "addicted" to HSPs. HSPs are overexpressed in many cancers due to upregulation of transcription factor Heat-shock factor 1 (HSF-1), the multifaceted master regulator of heat shock response. Therefore, pharmacological targeting of HSPs via HSF-1 is an effective strategy to treat malignant cancers like triple negative breast cancer...
July 17, 2017: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/28717596/phenotypic-and-microrna-transcriptomic-profiling-of-the-mda-mb-231-spheroid-enriched-cscs-with-comparison-of-mcf-7-microrna-profiling-dataset
#16
Lily Boo, Wan Yong Ho, Norlaily Mohd Ali, Swee Keong Yeap, Huynh Ky, Kok Gan Chan, Wai Fong Yin, Dilan Amila Satharasinghe, Woan Charn Liew, Sheau Wei Tan, Soon Keng Cheong, Han Kiat Ong
Breast cancer spheroids have been widely used as in vitro models of cancer stem cells (CSCs), yet little is known about their phenotypic characteristics and microRNAs (miRNAs) expression profiles. The objectives of this research were to evaluate the phenotypic characteristics of MDA-MB-231 spheroid-enriched cells for their CSCs properties and also to determine their miRNAs expression profile. Similar to our previously published MCF-7 spheroid, MDA-MB-231 spheroid also showed typical CSCs characteristics namely self-renewability, expression of putative CSCs-related surface markers and enhancement of drug resistance...
2017: PeerJ
https://www.readbyqxmd.com/read/28717100/inhibitory-modulation-of-human-estrogen-receptor-%C3%AE-and-%C3%AE-activities-by-dicyclohexyl-phthalate-in-human-breast-cancer-cell-lines
#17
Hiroyuki Okazaki, Shuso Takeda, Saki Matsuo, Masaya Matsumoto, Erika Furuta, Eriko Kohro-Ikeda, Hironori Aramaki
Phthalate esters (PAEs) are man-made compounds that are used widely in industry, and the ubiquitous exposure of humans to PAEs has been reported. Although some PAEs have been suggested to function as xenoestrogens in in vitro systems, such as human estrogen receptors (ERs) expressed in Chinese hamster ovary (CHO)-K1 cells, few studies have attempted to elucidate whether PAEs affect human ERα/ERβ-mediated signaling in human breast cancer cells (i.e., combination between human ERs and human cells). Thus, further experiments are needed in order to clarify the activities of PAEs...
2017: Journal of Toxicological Sciences
https://www.readbyqxmd.com/read/28716747/cysteine-as-a-potential-anti-amyloidogenic-agent-with-protective-ability-against-amyloid-induced-cytotoxicity
#18
Masihuz Zaman, Syed Mohammad Zakariya, Saima Nusrat, Tajalli Ilm Chandel, Syed Musthapa Meeran, Mohammad Rehan Ajmal, Parvez Alam, Wahiduzzaman, Rizwan Hasan Khan
Protein aggregation and misfolding have been allied with numerous human disorders and thus inhibition of such occurrence has been center for intense research efforts against these diseases. Here, we investigated anti-fibrillation activity of cysteine and its effect on kinetics of stem bromelain amyloid fibril formation. We established the anti-fibrillation and anti aggregation activities of cysteine by using multiple approaches like turbidity measurements, dye binding assays (ThT and ANS) and structural changes were monitored by circular dichroism (CD) followed by electron microscopy...
July 14, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28715872/a-novel-compound-lingh2-10-inhibits-the-growth-of-triple-negative-breast-cancer-cells-in-vitro-and-in-vivo-as-a-selective-inverse-agonist-of-estrogen-related-receptor-%C3%AE
#19
Yang Ning, Haifei Chen, Yongli Du, Hao Ling, Liudi Zhang, Lu Chen, Huijie Qi, Xiaojin Shi, Qunyi Li
Unlike other breast cancer subtypes, targeted therapies for triple negative breast cancer (TNBC) have yet to progress past clinical trial stage to approval. Accumulating evidences demonstrated that expression of estrogen-related receptor alpha (ERRα) indicated worse prognosis and correlated with poor outcome in breast cancers including TNBC. Therefore, ERRα modulators/regulators may be potential in the therapeutic treatment of breast cancers. In the current study, we presented a novel compound LingH2-10 that bound to ERRα, as identified using a time-resolved fluorescence resonance energy transfer assay (TR-FRET) with the IC50 value of 0...
July 13, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28714574/a-humanised-tissue-engineered-bone-model-allows-species-specific-breast-cancer-related-bone-metastasis-in-vivo
#20
V M C Quent, A V Taubenberger, J C Reichert, L C Martine, J A Clements, D W Hutmacher, D Loessner
Bone metastases frequently occur in the advanced stages of breast cancer. At this stage, the disease is deemed incurable. To date, the mechanisms of breast cancer-related metastasis to bone are poorly understood. This may be attributed to the lack of appropriate animal models to investigate the complex cancer cell-bone interactions. In this study, two established tissue-engineered bone constructs (TEBCs) were applied to a breast cancer-related metastasis model. A cylindrical medical-grade polycaprolactone-tricalcium phosphate scaffold produced by fused deposition modelling (scaffold 1) was compared with a tubular calcium phosphate-coated polycaprolactone scaffold fabricated by solution electrospinning (scaffold 2) for their potential to generate ectopic humanised bone in NOD/SCID mice...
July 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
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