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https://www.readbyqxmd.com/read/29783960/performance-of-oncoe6-cervical-test-with-collection-methods-enabling-self-sampling
#1
Amrei Krings, Anna M Dückelmann, Lutz Moser, Johannes Gollrad, Maarten Wiegerinck, Johannes Schweizer, Andreas M Kaufmann
BACKGROUND: The paradigm shift from cytological screening to Human Papillomavirus (HPV)-based screening for cervical cancer allows the introduction of new technologies in sample collection and diagnostics. The OncoE6™ Cervical Test (OncoE6 Test) is a rapid, easy-to-use lateral flow method detecting HPV16/18 E6 oncoproteins that has proven to detect high-grade cervical lesions with high specificity. If compatible with self-collection samples, this technology might allow for decentralized screening of hard-to-reach populations...
May 21, 2018: BMC Women's Health
https://www.readbyqxmd.com/read/29743199/evaluation-of-dried-blood-spots-and-oral-fluids-as-alternatives-to-serum-for-human-papillomavirus-antibody-surveillance
#2
Karly S Louie, Jama Dalel, Caroline Reuter, Sara L Bissett, Michelle Kleeman, Lesley Ashdown-Barr, Rawinder Banwait, Anna Godi, Peter Sasieni, Simon Beddows
Human papillomavirus (HPV) vaccination elicits high-titer genotype-specific antibody responses that are associated with a reduced risk of cervical disease caused by vaccine-incorporated genotypes. Our objective was to evaluate dried blood spots (DBSs) and oral mucosal transudate (OMT) as alternative samples to serum to confirm HPV vaccine antibody status. A study was carried out to evaluate the feasibility of detecting HPV16 and HPV18 antibodies in OMT, DBSs, and sera among women who self-reported being unvaccinated or fully vaccinated with the HPV vaccine...
June 27, 2018: MSphere
https://www.readbyqxmd.com/read/29708350/-human-papillomavirus-role-in-cervical-carcinogenesis-and-methods-of-detection
#3
Martin Bartošík, Roman Hrstka, Ludmila Jiráková
BACKGROUND: Persistent infection with high-risk human papillomavirus (HPV) strains, especially HPV 16 and HPV 18, is associated with the onset of various malignant diseases, including cervical carcinoma in women. HPV DNA testing is thus being implemented as a complementary method to standard cytological examination, mainly due to its increased sensitivity. AIM: This review outlines the role of HPV in cervical carcinogenesis, with a focus on the formation of cervical intraepithelial neoplasias (CIN1-3) and the molecular mechanism underlying cellular transformation...
2018: Klinická Onkologie: Casopis Ceské a Slovenské Onkologické Spolecnosti
https://www.readbyqxmd.com/read/29707519/molecular-signatures-of-high-grade-cervical-lesions
#4
Andreia M Porcari, Fernanda Negrão, Guilherme Lucas Tripodi, Denise Rocha Pitta, Elisabete Aparecida Campos, Douglas Munhoz Montis, Aline M A Martins, Marcos N Eberlin, Sophie F M Derchain
Cervical cancer is the fourth most common neoplasia in women and the infection with human papilloma virus (HPV) is its necessary cause. Screening methods, currently based on cytology and HPV DNA tests, display low specificity/sensitivity, reducing the efficacy of cervical cancer screening programs. Herein, molecular signatures of cervical cytologic specimens revealed by liquid chromatography-mass spectrometry (LC-MS), were tested in their ability to provide a metabolomic screening for cervical cancer. These molecules were tested whether they could clinically differentiate insignificant HPV infections from precancerous lesions...
2018: Frontiers in Oncology
https://www.readbyqxmd.com/read/29700570/-the-new-s3-guideline-prevention-of-cervical-carcinoma-what-is-important-for-pathology
#5
D Schmidt
Of the numerous aspects involved in the diagnosis, treatment, and follow-up of cervical uterine precursor lesions, epidemiology, virology, cytology, human papillomavirus (HPV) testing, and diagnostic algorithms for equivocal and HPV-positive findings are important for pathologists. Cytology will continue to be used as a preventive medical check-up in young women, while HPV-based screening is suggested for older women. HPV screening has yielded a significant reduction in cancer precursors and invasive cervical carcinoma in numerous studies...
April 26, 2018: Der Pathologe
https://www.readbyqxmd.com/read/29655523/the-clinical-value-of-hpv-e6-e7-and-stat3-mrna-detection-in-cervical-cancer-screening
#6
Yibing Fan, Zongji Shen
OBJECTIVE: To explore the value of human papillomavirus (HPV) E6/E7 and signal transducer and activator of transcription 3 (STAT3) mRNA detection in the screening of cervical lesions. METHODS: 192 patients with abnormal ThinPrep cytology test (TCT) results and/or high-risk HPV infection were screened to identify possible cervical lesions in cases. Diagnoses were confirmed by histopathology. Fluorescence in situ hybridization (FISH) was performed to detect and qualify the mRNAs of HPV E6/E7, STAT3, and Survivin in cervical exfoliated cells...
February 11, 2018: Pathology, Research and Practice
https://www.readbyqxmd.com/read/29566658/punch-biopsies-shorten-time-to-clearance-of-high-risk-human-papillomavirus-infections-of-the-uterine-cervix
#7
K U Petry, J Horn, A Luyten, R T Mikolajczyk
BACKGROUND: The primary objective was to determine human papilloma virus (HPV) clearance rate after cervical biopsy among women with persistent high-risk HPV infection compared with spontaneous HPV clearance rate in the absence of biopsy. METHODS: We collected data from a dedicated screening program of women aged 30-70 years old. Inclusion criteria for the baseline non-interventional cohort were a positive HPV test (hybrid capture 2, HC2) and normal cytology. In the baseline cohort women were followed with approximately yearly HPV-tests and cytology until HPV regressed (one negative HPV test) or interventions in the form of diagnostic biopsies or therapy...
March 23, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29566392/dual-p16-and-ki-67-expression-in-liquid-based-cervical-cytological-samples-compared-to-pap-cytology-findings-biopsies-and-hpv-testing-in-cervical-cancer-screening-a-diagnostic-accuracy-study
#8
COMPARATIVE STUDY
Karla Calaça Kabbach Prigenzi, Thaís Heinke, Rafael Calil Salim, Gustavo Rubino de Azevedo Focchi
OBJECTIVE: Our objective was to verify the sensitivity and specificity of dual immunocytochemistry staining for p16 and Ki-67 in liquid-based samples (the "dual" assay) for cervical lesion screening, compared to biopsy findings and human papillomavirus (HPV) DNA molecular detection. STUDY DESIGN: Sensitivity, specificity, and positive (PPV) and negative (NPV) predictive values for the "dual immunocytochemistry assay" were calculated and compared to histopathological results and to high-risk HPV DNA detection in adult women or teenagers submitted to cervical cancer screening...
2018: Acta Cytologica
https://www.readbyqxmd.com/read/29565690/cervical-screening-in-hpv-vaccinated-populations
#9
K Canfell
Cervical screening with cytology has been the basis for substantial reductions in cervical cancer incidence and mortality in most high-income countries over the last few decades. More recently, there have been two key, parallel developments which have prompted a major re-consideration of cervical screening. The first is the emergence of evidence on the improved sensitivity of human papillomavirus (HPV) DNA testing compared to cytology, and the second is the large-scale deployment of prophylactic vaccination against HPV...
March 22, 2018: Climacteric: the Journal of the International Menopause Society
https://www.readbyqxmd.com/read/29549429/integrated-morphologic-and-molecular-analysis-of-trichomonas-vaginalis-mycoplasma-hominis-and-human-papillomavirus-using-cytologic-smear-preparations
#10
I Nikas, A Hapfelmeier, M Mollenhauer, D Angermeier, M Bettstetter, R Götz, M Schmidmayr, V Seifert-Klauss, A Muckenhuber, U Schenck, Gregor Weirich
Pathogenic microbes may colonize the female genital tract via sexual transmission and cause health issues like inflammation or malignancy, summarized as sexually transmitted disease (STD). A major representative of such pathogens is Trichomonas vaginalis (T.v.), whose role in the etiology of cervical cancer remains elusive. Traditional morphologic screening of cervical smears is able to detect T.v., although its identification may be complicated by look-alikes such as degenerated granulocytes and basal cells...
May 2018: Parasitology Research
https://www.readbyqxmd.com/read/29491018/validation-of-a-human-papillomavirus-hpv-dna-cervical-screening-test-that-provides-expanded-hpv-typing
#11
Maria Demarco, Olivia Carter-Pokras, Noorie Hyun, Philip E Castle, Xin He, Cher M Dallal, Jie Chen, Julia C Gage, Brian Befano, Barbara Fetterman, Thomas Lorey, Nancy Poitras, Tina R Raine-Bennett, Nicolas Wentzensen, Mark Schiffman
As cervical cancer screening shifts from cytology to human papillomavirus (HPV) testing, a major question is the clinical value of identifying individual HPV types. We aimed to validate Onclarity (Becton Dickinson Diagnostics, Sparks, MD), a nine-channel HPV test recently approved by the FDA, by assessing (i) the association of Onclarity types/channels with precancer/cancer; (ii) HPV type/channel agreement between the results of Onclarity and cobas (Roche Molecular Systems, Pleasanton, CA), another FDA-approved test; and (iii) Onclarity typing for all types/channels compared to typing results from a research assay (linear array [LA]; Roche)...
May 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29454252/human-papillomavirus-detection-using-pcr-and-atr-ftir-for-cervical-cancer-screening
#12
Taciana Rymsza, Eliane Aline Ribeiro, Luis Felipe das Chagas E Silva de Carvalho, Tanmoy Bhattacharjee, Renata de Azevedo Canevari
The human papillomavirus (HPV) genital infection is considered one of the most common sexually transmitted diseases worldwide, and has been associated with cervical cancer. The objective of this study was to investigate the efficacy of the diagnostic methods: polymerase chain reaction (PCR) and Fourier transform infrared (FTIR) equipped with an ATR (Attenuated Total Reflectance) unit (Pike Tech) spectroscopy, to diagnose HPV infection in women undergoing gynecological examination. Seventeen patients (41.46%) of the 41 patients analyzed were diagnosed with exophytic/condyloma acuminate lesions by clinical analysis, 29 patients (70...
May 5, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29362127/sensitivity-and-specificity-of-oral-hpv-detection-for-hpv-positive-head-and-neck-cancer
#13
Brooke J Gipson, Hilary A Robbins, Carole Fakhry, Gypsyamber D'Souza
BACKGROUND: The incidence of HPV-related head and neck squamous cell carcinoma (HPV-HNSCC) is increasing. Oral samples are easy and non-invasive to collect, but the diagnostic accuracy of oral HPV detection methods for classifying HPV-positive HNSCC tumors has not been well explored. METHODS: In a systematic review, we identified eight studies of HNSCC patients meeting our eligibility criteria of having: (1) HPV detection in oral rinse or oral swab samples, (2) tumor HPV or p16 testing, (3) a publication date within the last 10 years (January 2007-May 2017, as laboratory methods change), and (4) at least 15 HNSCC cases...
February 2018: Oral Oncology
https://www.readbyqxmd.com/read/29353074/diagnostic-value-of-human-papillomavirus-hpv-16-and-hpv18-viral-loads-for-the-detection-of-high-grade-cervical-intraepithelial-neoplasia-cin2-in-a-cohort-of-african-women-living-with-hiv
#14
Michel Segondy, Jean Ngou, Helen Kelly, Tanvier Omar, Olga Goumbri-Lompo, Sylviane Doutre, Philippe Mayaud, Marie-Noelle Didelot
BACKGROUND: African women living with HIV (WLHIV) are at high risk of cervical cancer but rarely adequately screened. Better strategies enabling identification of WLHIV with high-grade cervical intraepithelial lesions (CIN2+) are required. OBJECTIVES: To investigate the diagnostic value of HPV16 and HPV18 viral loads in a cohort of African WLHIV. DESIGN: HPV16 and HPV18 viral loads were determined by quantitation of the E6 gene DNA by real-time PCR in cervical specimens collected at baseline and endline (16 months) from 245 African WLHIV positive for HPV16 or/and HPV18...
February 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/29328473/detection-of-high-grade-neoplasia-in-air-dried-cervical-pap-smears-by-a-microrna-based-classifier
#15
Mikhail K Ivanov, Sergei E Titov, Sergei A Glushkov, Victoria V Dzyubenko, Anastasia V Malek, Polina A Arkhangelskaya, Roman B Samsonov, Andrey A Mikhetko, Elena V Bakhidze, Igor V Berlev, Nikolay N Kolesnikov
Recent studies have shown that changes in the expression levels of certain microRNAs correlate with the degree of severity of cervical lesions. The aim of the present study was to develop a microRNA-based classifier for the detection of high-grade cervical intraepithelial neoplasia (CIN ≥2) in cytological samples from patients with different high-risk human papillomavirus (HR-HPV) viral loads. For this purpose, raw RT-qPCR data for 25 candidate microRNAs, U6 snRNA and human DNA in air-dried PAP smears from 174 women with different cervical cytological diagnoses, 144 of which were HR-HPV-positive [40 negative for intraepithelial lesion or malignancy (NILM), 34 low-grade squamous intraepithelial lesions (L-SIL), 57 high-grade squamous intraepithelial lesions (H-SIL), 43 invasive cancers], were statistically processed...
March 2018: Oncology Reports
https://www.readbyqxmd.com/read/29322839/feasibility-study-of-a-human-papillomavirus-e6-and-e7-oncoprotein-test-for-the-diagnosis-of-cervical-precancer-and-cancer
#16
Jin-Jun Zhang, Xin-Chun Cao, Xiang-Yu Zheng, Hai-Ying Wang, Yong-Wei Li
Objective To evaluate the clinical value of human papillomavirus (HPV) E6 and E7 oncoprotein (HPV E6/E7) detection in the early screening of cervical cancer. Methods This prospective study evaluated all patients with suspected cervical intraepithelial neoplasia (CIN) as identified by the presence of at least one positive indicator from a ThinPrep cytologic test (TCT) and/or a Hybrid Capture 2 (HC2) HPV DNA test. The levels of E6/E7 oncoproteins were determined using Western blot analysis. The diagnostic value of the HPV E6/E7 protein assay was compared with the clinical diagnosis from TCT, HC2 and the gold standard of cervical biopsy histology...
March 2018: Journal of International Medical Research
https://www.readbyqxmd.com/read/29299769/the-direct-medical-costs-of-diseases-associated-with-human-papillomavirus-infection-in-manitoba-canada
#17
Christiaan H Righolt, Gurpreet Pabla, Salaheddin M Mahmud
BACKGROUND: The total direct cost of screening and treating all human papillomavirus-related diseases (HPV-RD) has not been measured in a single study. Accurate cost estimates are needed to inform decisions on intervention priorities and evaluate the cost-effectiveness of existing programs. We used province-wide clinical, administrative, and accounting databases to measure direct medical costs of HPV infection in Manitoba (Canada). METHODS: All persons 9 years or older with health insurance coverage in Manitoba between April 2000 and March 2015 were eligible...
April 2018: Applied Health Economics and Health Policy
https://www.readbyqxmd.com/read/29299683/hpv-genotypes-detected-by-linear-array-and-next-generation-sequencing-in-anal-samples-from-hiv-positive-men-who-have-sex-with-men-in-mexico
#18
Luz A González-Hernández, María G Flores-Miramontes, Adriana Aguilar-Lemarroy, Krissya S Quintanilla-Peña, Fabiola L Martin-Amaya-Barajas, Moisés Ramos-Solano, Luis F Enciso Gómez, Jaime F Andrade-Villanueva, Luis F Jave-Suárez
The incidence of anal cancer has been rising, especially in HIV+ patients and has been associated with HPV infection. HIV+ patients are more at risk of HPV coinfection and are seven times more likely to have persistent HPV infection; moreover, HIV+ men have an increased risk of developing anal cancer compared to HIV+ women. The development of screening strategies for the detection of HPV in HIV+ men is of major importance; however, there is not enough information about the HPV genotypes and variants that are colonizing the anal epithelia of HIV+ men in diverse geographical regions...
April 2018: Archives of Virology
https://www.readbyqxmd.com/read/29290816/a-versatile-nanowire-platform-for-highly-efficient-isolation-and-direct-pcr-free-colorimetric-detection-of-human-papillomavirus-dna-from-unprocessed-urine
#19
HyungJae Lee, Mihye Choi, Sang-Hyun Hwang, Youngnam Cho
Purpose: As human papillomavirus (HPV) is primarily responsible for the development of cervical cancer, significant efforts have been devoted to develop novel strategies for detecting and identifying HPV DNA in urine. The analysis of target DNA sequences in urine offers a potential alternative to conventional methods as a non-invasive clinical screening and diagnostic assessment tool for the detection of HPV. However, the lack of efficient approaches to isolate and directly detect HPV DNA in urine has restricted its potential clinical use...
2018: Theranostics
https://www.readbyqxmd.com/read/29233547/the-developing-spectrum-of-gastric-type-cervical-glandular-lesions
#20
REVIEW
Karen L Talia, W Glenn McCluggage
Adenocarcinoma of the cervix is less common than squamous cell carcinoma, although its relative prevalence is increasing. Oncogenic (high-risk) human papillomavirus (HPV) infection is implicated in the development of approximately 90% of all cervical adenocarcinomas. Of the remaining non-HPV associated tumours, the most frequent is gastric-type adenocarcinoma (GAS), which is recognised by the World Health Organization as a form of mucinous adenocarcinoma of the cervix. Minimal deviation adenocarcinoma (MDA) of mucinous type (adenoma malignum) is considered an extremely well differentiated variant of GAS and is encompassed within the category of GAS...
February 2018: Pathology
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