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Hpv screening and diagnostic

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https://www.readbyqxmd.com/read/27922858/hiv-hpv-and-microbiota-partners-in-crime
#1
Sergio Serrano-Villar, Emilia Vásquez-Domínguez, José Antonio Pérez-Molina, Talía Sainz, Amparo de Benito, Amparo Latorre, Andrés Moya, María José Gosalbes, Santiago Moreno
Altered interplay between gut mucosa and dysbiotic bacteria during HIV infection seems to fuel chronic immune dysfunction and might explain the excess rates of HPV-associated anal cancer in HIV-infected individuals. Here, we show in HIV-infected MSM undergoing screening for HPV-related cancer that specific fecal and mucosal bacteria are able to predict the existence of precancerous anal lesions. If confirmed, these bacterial biomarkers could be exploited either as diagnostic tools or therapeutic targets.
December 5, 2016: AIDS
https://www.readbyqxmd.com/read/27826568/pax1-and-sox1-methylation-as-an-initial-screening-method-for-cervical-cancer-a-meta-analysis-of-individual-studies-in-asians
#2
Yan Chen, Zhaolei Cui, Zhenzhou Xiao, Minhua Hu, Chuanhui Jiang, Yingying Lin, Yansong Chen
BACKGROUND: Epigenetic alterations of gene or DNA methylation have been highlighted as promising biomarkers for early cervical cancer screening. Herein, we evaluated the diagnostic performance of paired boxed gene 1 (PAX1) and sex determining region Y-box 1 (SOX1) methylation for cervical cancer detection. METHODS: Eligible studies were retrieved by searching the electronic databases. Study quality was assessed according to the Quality Assessment of Diagnostic Accuracy Studies (QUADAS) checklist...
October 2016: Annals of Translational Medicine
https://www.readbyqxmd.com/read/27821079/comparison-of-dna-testing-strategies-in-monitoring-human-papillomavirus-infection-prevalence-through-simulation
#3
Carol Y Lin, Ling Li
BACKGROUND: HPV DNA diagnostic tests for epidemiology monitoring (research purpose) or cervical cancer screening (clinical purpose) have often been considered separately. Women with positive Linear Array (LA) polymerase chain reaction (PCR) research test results typically are neither informed nor referred for colposcopy. Recently, a sequential testing by using Hybrid Capture 2 (HC2) HPV clinical test as a triage before genotype by LA has been adopted for monitoring HPV infections. Also, HC2 has been reported as a more feasible screening approach for cervical cancer in low-resource countries...
November 7, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27816947/external-quality-assessment-for-human-papillomavirus-16-18-dna-detection-and-genotyping-in-shanghai-china
#4
Xueliang Wang, Fen Liu, Lingli Jiang, Yun Bao, Yanqun Xiao, Hualiang Wang
BACKGROUND: Human papillomavirus (HPV) DNA detection and genotyping is now being used for cervical screening by a growing number of laboratories in Shanghai, but they may have various levels of proficiency. The objective of this study was to evaluate the performance of clinical laboratories for HPV DNA detection and genotyping by an external quality assessment (EQA) program. METHODS: The EQA panels were clinically validated by the Cobas 4800 HPV test, and then distributed to the participating laboratories in May 2015 (round 1) and September 2015 (round 2)...
November 7, 2016: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/27806357/virtues-and-weaknesses-of-dna-methylation-as-a-test-for-cervical-cancer-prevention
#5
Attila T Lorincz
Epigenetics is the study of heritable and non-heritable genetic coding that is additive to information contained within classical DNA base pair sequences. Differential methylation has a fundamental role in the development and outcome of malignancies, chronic and degenerative diseases and aging. DNA methylation can be measured accurately and easily via various molecular methods and has become a key technology for research and healthcare delivery, with immediate roles in the elucidation of disease natural history, diagnostics and drug discovery...
November 3, 2016: Acta Cytologica
https://www.readbyqxmd.com/read/27761872/correlation-between-indoleamine-2-3-dioxygenase-mrna-and-cdkn2a-p16-mrna-a-combined-strategy-to-cervical-cancer-diagnosis
#6
Mario Cezar Saffi Junior, Ivone da Silva Duarte, Rodrigo Barbosa de Oliveira Brito, Giovana Garcia Prado, Sergio Makabe, Humberto Dellê, Cleber P Camacho
Cervical cancer (CC) is one of the most common cancers among women worldwide. The relation of the human papillomavirus (HPV) with CC and its precursor lesions was first suspected for over 40 years. The indoleamine 2,3 dioxygenase (IDO) is an immune modulator enzyme responsible for the immune system tissue protection mechanism, which may be the key to the tumoural persistence. HPV oncoprotein E7 promotes the increase in cyclin-dependent kinase inhibitor p16 (CDKN2A/p16). The isolated and combined analysis of CDKN2A/p16 mRNA to CC diagnosis was done with promising results...
November 2016: Medical Oncology
https://www.readbyqxmd.com/read/27727464/evaluation-of-multiple-primary-and-combination-screening-strategies-in-postmenopausal-women-for-detection-of-cervical-cancer-in-china
#7
Hunter K Holt, Li Zhang, Fang Hui Zhao, Shang Ying Hu, Xue Lian Zhao, Xun Zhang, Qin Jing Pan, Wen Hua Zhang, Jennifer S Smith, You Lin Qiao
As China's population ages, the importance of determining prevalence of cervical disease and accurate cervical cancer screening strategies for postmenopausal women is increasing.17 population-based studies were analyzed to determine prevalence of cervical neoplasia in postmenopausal women. All women underwent HPV DNA testing, visual inspection with acetic acid (VIA), and cytology testing. Diagnostic values for primary and combinations screening methods included sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), referral rate, and area under curve (AUC) were calculated using directed biopsy or 4 quadrants biopsy as reference standard...
October 11, 2016: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/27651839/molecular-pap-smear-hpv-genotype-and-dna-methylation-of-adcy8-cdh8-and-znf582-as-an-integrated-biomarker-for-high-grade-cervical-cytology
#8
Jane Shen-Gunther, Chiou-Miin Wang, Graham M Poage, Chun-Lin Lin, Luis Perez, Nancy A Banks, Tim Hui-Ming Huang
BACKGROUND: The Pap smear has remained the foundation for cervical cancer screening for over 70 years. With advancements in molecular diagnostics, primary high-risk human papillomavirus (hrHPV) screening has recently become an accepted stand-alone or co-test with conventional cytology. However, both diagnostic tests have distinct limitations. The aim of this study was to determine the association between HPV genotypes and cellular epigenetic modifications in three grades of cervical cytology for screening biomarker discovery...
2016: Clinical Epigenetics
https://www.readbyqxmd.com/read/27626178/diagnostic-accuracy-of-high-risk-hpv-genotyping-in-women-with-high-grade-cervical-lesions-evidence-for-renewed-the-cervical-cancer-screening-strategy-in-china
#9
Huihui Xu, Aifen Lin, Xiujuan Shao, Weiwu Shi, Yang Zhang, Weihua Yan
Currently, clinical data for primary HPV screening alone are lacking in China. Here, we evaluate cervical cancer screening with primary HPV genotyping, as well as possible future screening strategy. Overall, high-risk HPV (hrHPV) prevalence was 18.2% among hospital-based population in Taizhou area. For cervical intraepithelial neoplasia 2 or worse (CIN2+), the sensitivity of primary hrHPV genotyping strategy and current cervical cancer screening strategy were 93.5%, and 71.1%, respectively; whereas the specificity was 17...
September 10, 2016: Oncotarget
https://www.readbyqxmd.com/read/27618102/current-technologies-and-recent-developments-for-screening-of-hpv-associated-cervical-and-oropharyngeal-cancers
#10
REVIEW
Sunny S Shah, Satyajyoti Senapati, Flora Klacsmann, Daniel L Miller, Jeff J Johnson, Hsueh-Chia Chang, M Sharon Stack
Mucosal infection by the human papillomavirus (HPV) is responsible for a growing number of malignancies, predominantly represented by cervical cancer and oropharyngeal squamous cell carcinoma. Because of the prevalence of the virus, persistence of infection, and long latency period, novel and low-cost methods are needed for effective population level screening and monitoring. We review established methods for screening of cervical and oral cancer as well as commercially-available techniques for detection of HPV DNA...
September 9, 2016: Cancers
https://www.readbyqxmd.com/read/27603593/immediate-referral-to-colposcopy-versus-cytological-surveillance-for-low-grade-cervical-cytological-abnormalities-in-the-absence-of-hpv-test-a-systematic-review-and-a-meta-analysis-of-the-literature
#11
Maria Kyrgiou, Ilkka Kalliala, Anita Mitra, Ka Ying Bonnie Ng, Olivia Raglan, Christina Fotopoulou, Pierre Martin-Hirsch, Evangelos Paraskevaidis, Marc Arbyn
We performed a systematic review and meta-analysis to explore the optimum management strategy for women with atypical squamous cells of undetermined significance (ASCUS/borderline) or low-grade squamous intra-epithelial lesions (LSIL/mild dyskaryosis) cytological abnormalities at primary screening in the absence of HPV DNA test. We searched MEDLINE, EMBASE and CENTRAL and included randomised controlled trials comparing immediate colposcopy to cytological surveillance in women with ASCUS/LSIL. The outcomes of interest were occurrence of different histological grades of cervical intraepithelial neoplasia (CIN) and default rates during follow-up...
January 1, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/27588487/p16-ki-67-co-expression-associates-high-risk-human-papillomavirus-persistence-and-cervical-histopathology-a-3-year-cohort-study-in-china
#12
Lu-Lu Yu, Hui-Qin Guo, Xiao-Qin Lei, Yu Qin, Ze-Ni Wu, Le-Ni Kang, Xun Zhang, You-Lin Qiao, Wen Chen
PURPOSE: To evaluate the association of p16/Ki-67 co-expression and persistence of high-risk human papillomavirus (HR-HPV) infection as well as cervical abnormalities. METHODS: We performed a 3-year cohort study among which 2498 Chinese women aged 25 to 65 years were screened by different HPV tests in 2011. 690 women who were positive at any of the tests and a random sample of 164 women with all negative results received colposcopy, cervical specimens for cobas HPV test (Roche diagnostics) were collected before colposcopy; of this group, 737 cervical specimens were collected to perform cobas, Liquid-based cytology, HPV E6 test (Arbor Vita Corporation) and p16/Ki-67 dual staining (Roche diagnostics) in 2014...
August 30, 2016: Oncotarget
https://www.readbyqxmd.com/read/27578366/relevance-of-the-pap-test-a-report-of-hpv-dna-test-negative-high-grade-squamous-intraepithelial-lesions-of-the-female-lower-genital-tract
#13
Yiang Hui, Katrine Hansen, Jayasimha Murthy, Danielle Chau, C James Sung, M Ruhul Quddus
OBJECTIVE: A vast majority of cervicovaginal intraepithelial lesions are caused by high-risk human papillomaviruses (HPVs). The Pap test has been the sole method used for the screening of cervicovaginal squamous intraepithelial lesions (SIL). Recently, the FDA approved an HPV-DNA assay as a method of primary screening. We report on a series of FDA-approved HPV-DNA test-negative SIL with HPV genotyping, using an alternative method on the corresponding surgical biopsy specimens. STUDY DESIGN: A retrospective review identified cytology-positive HPV-negative cases over a 15-month period at a tertiary care gynecologic oncology institution...
2016: Acta Cytologica
https://www.readbyqxmd.com/read/27568665/p16ink4a-a-potential-diagnostic-adjunct-for-prediction-of-high-grade-cervical-lesions-in-liquid-based-cytology-with-hpv-testing-and-histological-correlation
#14
Y P Wong, S H Abdul Raub, A Z H Mohd Dali, F Kassim, V Visvalingam, Z Zakaria, M A Kamaluddin, S Noor Akmal
Human papillomavirus (HPV) is a necessary cause of cervical cancer and its precursors. Increased expression of high-risk hrHPV viral oncogenes in abnormal cells might increase the expression of p16INK4a. We aimed to determine the role of p16INK4a in detecting hrHPV-transformed epithelial cells in liquid-based cervical cytology, and compared the results with hrHPV DNA testing by realtime polymerase chain reaction (RT-PCR). Fifty-seven cytological samples were tested for p16INK4a immunomarker and hrHPV DNA. Test performance of both tests was determined by comparing sensitivity, specificity and predictive values using available histological follow-up data as gold standard...
August 2016: Malaysian Journal of Pathology
https://www.readbyqxmd.com/read/27497597/the-value-of-a-novel-panel-of-cervical-cancer-biomarkers-for-triage-of-hpv-positive-patients-and-for-detecting-disease-progression
#15
Norbert Varga, Johanna Mózes, Helen Keegan, Christine White, Lynne Kelly, Loretto Pilkington, Márta Benczik, Schaff Zsuzsanna, Gábor Sobel, Róbert Koiss, Edit Babarczi, Miklos Nyíri, Laura Kovács, Sebe Attila, Borbála Kaltenecker, Adrienn Géresi, Adrienn Kocsis, John O'Leary, Cara M Martin, Csaba Jeney
In the era of primary vaccination against HPV and at the beginning of the low prevalence of cervical lesions, introduction of screening methods that can distinguish between low- and high-grade lesions is necessary in order to maintain the positive predictive value of screening. This case-control study included 562 women who attended cervical screening or were referred for colposcopy and 140 disease free controls, confirmed by histology and/or cytology. The cases were stratified by age. Using routine exfoliated liquid based cytological samples RT-PCR measurements of biomarker genes, high-risk HPV testing and liquid based cytology were performed and used to evaluate different testing protocols including sets of genes/tests with different test cut-offs for the diagnostic panels...
August 6, 2016: Pathology Oncology Research: POR
https://www.readbyqxmd.com/read/27468883/detection-of-oncogenic-mutations-in-cervical-carcinoma-using-method-high-resolution-melting-hrm
#16
M Wayhelova, A Mikulasova, J Smetana, V Vallova, D Blazkova, H Filkova, L Moukova, P Kuglik
Oncogenic mutations in proto-oncogenes and tumor suppressor genes represent one of key events in cancerogenesis. In this study, we analysed mutation status in PIK3CA, KRAS and EGFR proto-oncogenes and TP53 tumor suppressor gene in a cohort of twenty-four patients diagnosed with squamous cell carcinoma or adenocarcinoma using the screening method "High Resolution Melting" (HRM). Positive findings were confirmed and identified by Sanger sequencing. Totally, we detected DNA sequence changes in targeted regions in seven patients (7/24, 29...
2016: Neoplasma
https://www.readbyqxmd.com/read/27450917/genetic-variability-and-lineage-phylogeny-of-human-papillomavirus-type-16-and-53-based-on-the-e6-e7-and-l1-genes-in-southwest-china
#17
Man Cao, Yuwei Chenzhang, Xianping Ding, Yiwen Zhang, Yaling Jing, Zuyi Chen
Human papillomaviruses (HPVs) are circular double-stranded DNA viruses that are highly prevalent in the general population, and account for the cervical cancer burden in women worldwide. In this study, we analyzed HPV-16, the most prevalent type worldwide, and HPV-53, a possible high-risk type from infected women in Southwest China. To characterize mutations, intratypic variants, and genetic variability in the E6, E7, and L1 genes of HPV-16 (n=97) and HPV-53 (n=15), these genes were sequenced and submitted to GenBank...
October 30, 2016: Gene
https://www.readbyqxmd.com/read/27446578/a-comparison-between-pap-and-hpv-screening-tests-and-screening-methods
#18
Emma Altobelli, Giorgio Scarselli, Amedeo Lattanzi, Carmine Fortunato, Valerio F Profeta
The present study assesses the results of cervical cancer (CC) screening over two 3-year periods (2008-2010 and 2011-2013) by comparing two screening tests [Papanicolaou (Pap) and human papillomavirus (HPV) tests] and two screening methods (organized and spontaneous). The study population includes women aged 25-64 years who underwent CC screening between 2008 and 2010 and/or 2011 and 2013, divided into those who responded to an invitation letter (organized screening) and those who spontaneously underwent testing at a public or private facility (non-programmed screening)...
August 2016: Molecular and Clinical Oncology
https://www.readbyqxmd.com/read/27446364/evaluation-of-3d-cpa-hr-hpv-and-tct-joint-detection-on-cervical-disease-screening
#19
Hui Liang, Min Fu, Jian Zhou, Lei Song
The application value of three-dimensional color power angiography (3D-CPA), high-risk human papillomavirus (HR-HPV), ThinPrep cytology test (TCT) joint detection on cervical disease screening was investigated. In total, 1,900 patients that were examined in Gynecological and Cervix Clinic of Maternal and Child Care Service Center of Xuzhou from June 2012 to March 2015 were enrolled in the present study. After admission, the patients underwent TCT, HR-HPV and 3D-CPA examinations, and vascular morphology and typing, vascularization index (VI) were recorded...
August 2016: Oncology Letters
https://www.readbyqxmd.com/read/27391265/prevalence-of-sexually-transmitted-viral-and-bacterial-infections-in-hiv-positive-and-hiv-negative-men-who-have-sex-with-men-in-toronto
#20
Robert S Remis, Juan Liu, Mona R Loutfy, Wangari Tharao, Anuradha Rebbapragada, Sanja Huibner, Maya Kesler, Roberta Halpenny, Troy Grennan, Jason Brunetta, Graham Smith, Tatjana Reko, Rupert Kaul
BACKGROUND: Hepatitis B (HBV), hepatitis C (HCV) and other sexually transmitted infections (STIs) have been associated with HIV transmission risk and disease progression among gay men and other men who have sex with men (MSM), but the frequency and distribution of STIs in this community in Canada has not been extensively studied. METHODS: We recruited MSM living with and without HIV from a large primary care clinic in Toronto. Participants completed a detailed socio-behavioural questionnaire using ACASI and provided blood for syphilis, HIV, HBV and HCV, herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2), and human cytomegalovirus (CMV) serology, urine for chlamydia and gonorrhea, and a self-collected anal swab for human papillomavirus (HPV) molecular diagnostics...
2016: PloS One
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