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Caveolin1

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https://www.readbyqxmd.com/read/27466374/p-glycoprotein-traffics-from-the-nucleus-to-the-plasma-membrane-in-rat-brain-endothelium-during-inflammatory-pain
#1
Margaret E Tome, Joseph M Herndon, Charles P Schaefer, Leigh M Jacobs, Yifeng Zhang, Chelsea K Jarvis, Thomas P Davis
P-glycoprotein (PgP), a drug efflux pump in blood-brain barrier endothelial cells, is a major clinical obstacle for effective central nervous system drug delivery. Identifying PgP regulatory pathways that can be exploited clinically is critical for improving central nervous system drug delivery. We previously found that PgP activity increases in rat brain microvessels concomitant with decreased central nervous system drug delivery in response to acute peripheral inflammatory pain. In the current study, we tested the hypothesis that PgP traffics to the luminal plasma membrane of the microvessel endothelial cells from intracellular stores during peripheral inflammatory pain...
November 2016: Journal of Cerebral Blood Flow and Metabolism
https://www.readbyqxmd.com/read/27022017/do-src-kinase-and-caveolin-interact-directly-with-na-k-atpase
#2
Eliyahu Yosef, Adriana Katz, Yoav Peleg, Tevie Mehlman, Steven J D Karlish
Much evidence points to a role of Na,K-ATPase in ouabain-dependent signal transduction. Based on experiments with different cell lines and native tissue membranes, a current hypothesis postulates direct interactions between the Na,K-ATPase and Src kinase (non-receptor tyrosine kinase). Na,K-ATPase is proposed to bind Src kinase and inhibit its activity, whereas ouabain, the specific Na,K-ATPase inhibitor, binds and stabilizes the E2 conformation, thus exposing the Src kinase domain and its active site Tyr-418 for activation...
May 27, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/26662566/evaluation-of-hepatic-vascular-endothelial-injury-during-liver-storage-by-molecular-detection-and-targeted-contrast-enhanced-ultrasound-imaging
#3
Fang Xie, Shu-Hua Zhang, Jia Cheng, Hong-Wei Wang, Xiang Fei, Zi-Yu Jiao, Jie Tang, Yu-Kun Luo
We hypothesized that lack of the high-energy phosphates during liver storage may potentially cause persistent injury to the vascular endothelium. Biopsies were obtained from livers obtained from beating heart human donors, stored either in the standard storage solution, that is, University of Wisconsin solution (UWS) or Celsior, and examined for various markers related to progressive endothelial injury. The expression of P2Y1 receptor, the major signal transduction machinery for adenosine triphosphate/adenosine diphosphate, decreased in hepatic vascular endothelial cells over time...
January 2016: IUBMB Life
https://www.readbyqxmd.com/read/26176221/a-frame-shift-mutation-in-cav1-is-associated-with-a-severe-neonatal-progeroid-and-lipodystrophy-syndrome
#4
Isabelle Schrauwen, Szabolcs Szelinger, Ashley L Siniard, Ahmet Kurdoglu, Jason J Corneveaux, Ivana Malenica, Ryan Richholt, Guy Van Camp, Matt De Both, Shanker Swaminathan, Mari Turk, Keri Ramsey, David W Craig, Vinodh Narayanan, Matthew J Huentelman
A 3-year-old female patient presenting with an unknown syndrome of a neonatal progeroid appearance, lipodystrophy, pulmonary hypertension, cutis marmorata, feeding disorder and failure to thrive was investigated by whole-genome sequencing. This revealed a de novo, heterozygous, frame-shift mutation in the Caveolin1 gene (CAV1) (p.Phe160X). Mutations in CAV1, encoding the main component of the caveolae in plasma membranes, cause Berardinelli-Seip congenital lipodystrophy type 3 (BSCL). Although BSCL is recessive, heterozygous carriers either show a reduced phenotype of partial lipodystrophy, pulmonary hypertension, or no phenotype...
2015: PloS One
https://www.readbyqxmd.com/read/26055893/selectivity-of-commonly-used-inhibitors-of-clathrin-mediated-and-caveolae-dependent-endocytosis-of-g-protein-coupled-receptors
#5
Shuohan Guo, Xiaohan Zhang, Mei Zheng, Xiaowei Zhang, Chengchun Min, Zengtao Wang, Seung Hoon Cheon, Min-Ho Oak, Seung-Yeol Nah, Kyeong-Man Kim
Among the multiple G protein-coupled receptor (GPCR) endocytic pathways, clathrin-mediated endocytosis (CME) and caveolar endocytosis are more extensively characterized than other endocytic pathways. A number of endocytic inhibitors have been used to block CME; however, systemic studies to determine the selectivity of these inhibitors are needed. Clathrin heavy chain or caveolin1-knockdown cells have been employed to determine the specificity of various chemical and molecular biological tools for CME and caveolar endocytosis...
October 2015: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/25933830/cell-specific-uptake-of-mantle-cell-lymphoma-derived-exosomes-by-malignant-and-non-malignant-b-lymphocytes
#6
Inbal Hazan-Halevy, Daniel Rosenblum, Shiri Weinstein, Osnat Bairey, Pia Raanani, Dan Peer
Mantle cell lymphoma (MCL) is an aggressive and incurable mature B cell neoplasm. The current treatments are based on chemotherapeutics and new class of drugs (e.g. Ibrutinib(®)), which in most cases ends with tumor resistance and relapse. Therefore, further development of novel therapeutic modalities is needed. Exosomes are natural extracellular vesicles, which play an important role in intercellular communication. The specificity of exosome uptake by different target cells remains unknown. In this study, we observed that MCL exosomes are taken up rapidly and preferentially by MCL cells...
August 1, 2015: Cancer Letters
https://www.readbyqxmd.com/read/25848967/an-a%C3%AE-42-uptake-and-degradation-via-rg3-requires-an-activation-of-caveolin-clathrin-and-a%C3%AE-degrading-enzymes-in-microglia
#7
Su Kil Jang, Jung Min Yu, Seung Tae Kim, Gwang Hoon Kim, Da Woon Park, Do Ik Lee, Seong Soo Joo
We demonstrated previously that ginsenoside Rg3 enhances the expression of macrophage scavenger receptor class A (SRA) and amyloid β peptide 1-42 (Aβ42) uptake in BV2 cells. In this study, we investigated the biochemical and mechanistic roles of Rg3 in human microglia and animal models to identify the determinants that participate in restoring memory and learning in brains disrupted by the Aβ42 peptide. SRA was expressed highly in Rg3-treated rats, and learning and memory functions were maintained at a normal level after the infusion of Aβ42...
July 5, 2015: European Journal of Pharmacology
https://www.readbyqxmd.com/read/25832806/identification-of-p-glycoprotein-co-fractionating-proteins-and-specific-binding-partners-in-rat-brain-microvessels
#8
Margaret E Tome, Charles P Schaefer, Leigh M Jacobs, Yifeng Zhang, Joseph M Herndon, Fabian O Matty, Thomas P Davis
Drug delivery to the brain for the treatment of pathologies with a CNS component is a significant clinical challenge. P-glycoprotein (PgP), a drug efflux pump in the endothelial cell membrane, is a major factor in preventing therapeutics from crossing the blood-brain barrier (BBB). Identifying PgP regulatory mechanisms is key to developing agents to modulate PgP activity. Previously, we found that PgP trafficking was altered concomitant with increased PgP activity and disassembly of high molecular weight PgP-containing complexes during acute peripheral inflammatory pain...
July 2015: Journal of Neurochemistry
https://www.readbyqxmd.com/read/25588833/cavin3-interacts-with-cavin1-and-caveolin1-to-increase-surface-dynamics-of-caveolae
#9
Jagan Mohan, Björn Morén, Elin Larsson, Mikkel R Holst, Richard Lundmark
Caveolae are invaginations of the cell surface thought to regulate membrane tension, signalling, adhesion and lipid homeostasis owing to their dynamic behaviour ranging from stable surface association to dynamic rounds of fission and fusion with the plasma membrane. The caveolae coat is generated by oligomerisation of the membrane protein caveolin and the family of cavin proteins. Here, we show that cavin3 (also known as PRKCDBP) is targeted to caveolae by cavin1 (also known as PTRF) where it interacts with the scaffolding domain of caveolin1 and promote caveolae dynamics...
March 1, 2015: Journal of Cell Science
https://www.readbyqxmd.com/read/25530524/-bone-and-calcium-research-update-2015-intracellular-ca2-regulation-in-endothelial-cells
#10
REVIEW
Masashi Isshiki
Endothelial cells regulates vascular tonus and exerts anti-atherosclerotic effects in response to external stimulation via Ca2+-dependent production of vasoactive substances such as NO. Agonists such as ATP hydrolyze membrane PIP2 and release soluble IP3 into the cell. IP3 then binds to the IP3 receptor and mobilizes Ca2+ from endoplasmic reticulum Ca2+ stores, followed by Ca2+ influxes from the extracellular space. Such store-operated Ca2+ entry comprises organization of a Ca2+ signal complex at the local subplasmalemmal domain involving TRPC4, caveolin1, and STIM1, a Ca2+ sensor protein for intracellular Ca2+ stores...
January 2015: Clinical Calcium
https://www.readbyqxmd.com/read/25434996/influence-of-membrane-cholesterol-in-the-molecular-evolution-and-functional-regulation-of-trpv4
#11
Shikha Kumari, Ashutosh Kumar, Puspendu Sardar, Manoj Yadav, Rakesh Kumar Majhi, Abhishek Kumar, Chandan Goswami
TRPV4 is involved in several physiological and sensory functions as well as with several diseases and genetic disorders, though the molecular mechanisms for these are unclear. In this work we have analyzed molecular evolution and structure-function relationship of TRPV4 using sequences from different species. TRPV4 has evolved during early vertebrate origin (450million years). Synteny analysis confirms that TRPV4 has coevolved with two enzymes involved in sterol biosynthesis, namely MVK and GLTP. Cholesterol-recognizing motifs are present within highly conserved TM4-Loop4-TM5 region of TRPV4...
January 2, 2015: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/25312970/aberrant-mir199a-5p-caveolin1-ppar%C3%AE-axis-in-hepatic-steatosis
#12
Bo Li, Zhiguo Zhang, Huizhi Zhang, Kai Quan, Yan Lu, Dongsheng Cai, Guang Ning
The prevalence of non-alcoholic fatty liver disease (NAFLD), a condition characterized by an excessive accumulation of triglycerides (TGs) in hepatocytes, has dramatically increased globally during recent decades. MicroRNAs (miRs) have been suggested to play crucial roles in many complex diseases and lipid metabolism. Our results indicated that miR199a-5p was remarkably upregulated in free fatty acid (FA)-treated hepatocytes. To investigate the role of miR199a-5p in the pathogenesis of fatty liver and the potential mechanism by which miR199a-5p regulates NAFLD, we first transfected two hepatocyte cell lines, HepG2 and AML12 cells, with agomiR199a-5p or antagomiR199a-5p...
December 2014: Journal of Molecular Endocrinology
https://www.readbyqxmd.com/read/24917030/a-microchip-filter-device-incorporating-slit-arrays-and-3-d-flow-for-detection-of-circulating-tumor-cells-using-cav1-epcam-conjugated-microbeads
#13
Yeon Jeong Kim, Gi-Bang Koo, June-Young Lee, Hui-Sung Moon, Dong-Gun Kim, Da-Gyum Lee, Ju-Yeon Lee, Jin Ho Oh, Jong-Myeon Park, Minseok S Kim, Hyun Goo Woo, Seung-Il Kim, Pilsung Kang, Wonshik Choi, Tae Seok Sim, Woong-Yang Park, Jeong-Gun Lee, You-Sun Kim
Circulating tumor cells (CTCs) are rare cells and the presence of these cells may indicate a poor prognosis and a high potential for metastasis. Despite highly promising clinical applications, CTCs have not been investigated thoroughly, due to many technical limitations faced in their isolation and identification. Current CTC detection techniques mostly take the epithelial marker epithelial cell adhesion molecule (EpCAM), however, accumulating evidence suggests that CTCs show heterogeneous EpCAM expression due to the epithelial-to-mesenchymal transition (EMT)...
August 2014: Biomaterials
https://www.readbyqxmd.com/read/24844382/uptake-of-shiga-toxigenic-escherichia-coli%C3%A2-subab-by-hela-cells-requires-an-actin-and-lipid-raft-dependent-pathway
#14
Sayaka Nagasawa, Kohei Ogura, Hiroyasu Tsutsuki, Hisako Saitoh, Joel Moss, Hirotaro Iwase, Masatoshi Noda, Kinnosuke Yahiro
The novel cytotoxic factor subtilase cytotoxin (SubAB) is produced mainly by non-O157 Shiga-toxigenic Escherichia coli (STEC). SubAB cleaves the molecular chaperone BiP/GRP78 in the endoplasmic reticulum (ER), leading to activation of RNA-dependent protein kinase (PKR)-like ER kinase (PERK), followed by caspase-dependent cell death. However, the SubAB uptake mechanism in HeLa cells is unknown. In this study, a variety of inhibitors and siRNAs were employed to characterize the SubAB uptake process. SubAB-induced BiP cleavage was inhibited by high concentrations of Dynasore, and methyl-β-cyclodextrin (mβCD) and Filipin III, but not suppressed in clathrin-, dynamin I/II-, caveolin1- and caveolin2-knockdown cells...
October 2014: Cellular Microbiology
https://www.readbyqxmd.com/read/24747515/polymerase-i-and-transcript-release-factor-acts-as-an-essential-modulator-of-glioblastoma-chemoresistance
#15
Xin Wang, Tianzhu Liu, Yifeng Bai, Hongzhan Liao, Shengcong Qiu, Zhenhua Chang, Yanting Liu, Xiaohui Yan, Hongbo Guo
OBJECTIVES: This study is to investigate if polymerase I and transcript release factor (PTRF) acts as a modulator in glioblastoma (GBM) chemoresistance. METHODS: Multidrug resistant (MDR) GBM cell line U251AR was established by exposing the U251 cell line to imatinib. The 2D-DIGE and MALDI-TOF/TOF-MS were performed on U251 and U251AR cell lines to screen MDR-related proteins. The expression of PTRF was determined by Western blot and quantitative RT-PCR analyses...
2014: PloS One
https://www.readbyqxmd.com/read/24628852/expression-and-activity-of-rac1-is-negatively-affected-in-the-dehydroepiandrosterone-induced-polycystic-ovary-of-mouse
#16
Vineet Kumar Maurya, Chadchan Sangappa, Vijay Kumar, Sahil Mahfooz, Archana Singh, Singh Rajender, Rajesh Kumar Jha
BACKGROUND: Polycystic ovarian syndrome (PCOS) is characterized by the presence of multiple follicular cysts, giving rise to infertility due to anovulation. This syndrome affects about 10% of women, worldwide. The exact molecular mechanism leading to PCOS remains obscure. RhoGTPase has been associated with oogenesis, but its role in PCOS remains unexplored. Therefore, we attempted to elucidate the Vav-Rac1 signaling in PCOS mice model. METHODS: We generated a PCOS mice model by injecting dehydroepiandrosterone (DHEA) for a period of 20 days...
2014: Journal of Ovarian Research
https://www.readbyqxmd.com/read/23843039/the-dioxin-receptor-has-tumor-suppressor-activity-in-melanoma-growth-and-metastasis
#17
María Contador-Troca, Alberto Alvarez-Barrientos, Eva Barrasa, Eva M Rico-Leo, Inmaculada Catalina-Fernández, Mauricio Menacho-Márquez, Xosé R Bustelo, José C García-Borrón, Aurea Gómez-Durán, Javier Sáenz-Santamaría, Pedro M Fernández-Salguero
Melanoma is a highly metastatic and malignant skin cancer having poor rates of patient survival. Since the incidence of melanoma is steadily increasing in the population, finding prognostic and therapeutic targets are crucial tasks in cancer. The dioxin receptor (AhR) is required for xenobiotic-induced toxicity and carcinogenesis and for cell physiology and organ homeostasis. Yet, the mechanisms by which AhR affects tumor growth and dissemination are largely uncharacterized. We report here that AhR contributes to the tumor-stroma interaction, blocking melanoma growth and metastasis when expressed in the tumor cell but supporting melanoma when expressed in the stroma...
December 2013: Carcinogenesis
https://www.readbyqxmd.com/read/23634221/cavin1-a-regulator-of-lung-function-and-macrophage-phenotype
#18
Praveen Govender, Freddy Romero, Dilip Shah, Jesus Paez, Shi-Ying Ding, Libin Liu, Adam Gower, Elizabeth Baez, Sherif Shawky Aly, Paul Pilch, Ross Summer
Caveolae are cell membrane invaginations that are highly abundant in adipose tissue, endothelial cells and the lung. The formation of caveolae is dependent on the expression of various structural proteins that serve as scaffolding for these membrane invaginations. Cavin1 is a newly identified structural protein whose deficiency in mice leads to loss of caveolae formation and to development of a lipodystrophic phenotype. In this study, we sought to investigate the functional role of Cavin1 in the lung. Cavin1 deficient mice possessed dramatically altered distal lung morphology and exhibited significant physiological alterations, notably, increased lung elastance...
2013: PloS One
https://www.readbyqxmd.com/read/23430896/a-patient-with-congenital-generalized-lipodystrophy-due-to-a-novel-mutation-in-bscl2-indications-for-secondary-mitochondrial-dysfunction
#19
Ellen H Jeninga, Monique de Vroede, Nicole Hamers, Johannes M P J Breur, Nanda M Verhoeven-Duif, Ruud Berger, Eric Kalkhoven
BACKGROUND: Congenital generalized lipodystrophy (CGL) results from mutations in AGPAT2, encoding 1-acyl-glycerol-3-phosphate-acyltransferase 2 (CGL1; MIM 608594), BSCL2, encoding seipin (CGL2; MIM 269700), CAV1, encoding caveolin1 (CGL3; MIM 612526) or PTRF, encoding polymerase I and transcript release factor (CGL4; MIM 613327). This study aims to investigate the genotype/phenotype relationship and search for a possible pathogenic mechanism in a patient with CGL. DESIGN: Case report...
2012: JIMD Reports
https://www.readbyqxmd.com/read/23203809/impairment-of-trpc1-stim1-channel-assembly-and-aqp5-translocation-compromise-agonist-stimulated-fluid-secretion-in-mice-lacking-caveolin1
#20
Biswaranjan Pani, Xibao Liu, Sunitha Bollimuntha, Kwong Tai Cheng, Ingrid R Niesman, Changyu Zheng, Virginia R Achen, Hemal H Patel, Indu S Ambudkar, Brij B Singh
Neurotransmitter regulation of salivary fluid secretion is mediated by activation of Ca(2+) influx. The Ca(2+)-permeable transient receptor potential canonical 1 (TRPC1) channel is crucial for fluid secretion. However, the mechanism(s) involved in channel assembly and regulation are not completely understood. We report that Caveolin1 (Cav1) is essential for the assembly of functional TRPC1 channels in salivary glands (SG) in vivo and thus regulates fluid secretion. In Cav1(-/-) mouse SG, agonist-stimulated Ca(2+) entry and fluid secretion are significantly reduced...
January 15, 2013: Journal of Cell Science
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