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https://www.readbyqxmd.com/read/28326334/bactericide-immunomodulating-and-wound-healing-properties-of-transgenic-kalanchoe-pinnata-synergize-with-antimicrobial-peptide-cecropin-p1-in-vivo
#1
A A Lebedeva, N S Zakharchenko, E V Trubnikova, O A Medvedeva, T V Kuznetsova, G A Masgutova, M V Zylkova, Y I Buryanov, A S Belous
Procedure of manufacturing K. pinnata water extracts containing cecropin P1 (CecP1) from the formerly described transgenic plants is established. It included incubation of leaves at +4°C for 7 days, mechanical homogenization of leaves using water as extraction solvent, and heating at +70°C for inactivating plant enzymes. Yield of CecP1 (after heating and sterilizing filtration) was 0.3% of total protein in the extract. The water extract of K. pinnata + CecP1 exhibits favorable effect on healing of wounds infected with S...
2017: Journal of Immunology Research
https://www.readbyqxmd.com/read/28223215/bufadienolides-from-kalanchoe-daigremontiana-as-thrombin-inhibitors-in-vitro-and-in-silico-study
#2
Joanna Kolodziejczyk-Czepas, Malgorzata Sieradzka, Barbara Moniuszko-Szajwaj, Łukasz Pecio, Michal B Ponczek, Pawel Nowak, Anna Stochmal
Thrombin is an active plasma coagulation factor II, critical for the formation of fibrin clot during blood coagulation. For that reason, this protein is also a crucial target for different anti-thrombotic therapies. The work is based on in vitro evaluation of the inhibitory effect of bufadienolide-rich fraction, isolated from roots of Kalanchoe daigremontiana (1-50μg/ml) on enzymatic properties of a serine proteinase - thrombin. The efficacy of the inhibition of amidolytic activity of thrombin (measured as a hydrolysis of the chromogenic substrate S-2238, Chromogenix) attained about 10 and 66%, respectively...
February 20, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28207897/correction-inhibitory-effects-of-hydroethanolic-leaf-extracts-of-kalanchoe-brasiliensis-and-kalanchoe-pinnata-crassulaceae-against-local-effects-induced-by-bothrops-jararaca-snake-venom
#3
Júlia Morais Fernandes, Juliana Félix-Silva, Lorena Medeiros da Cunha, Jacyra Antunes Dos Santos Gomes, Emerson Michell da Silva Siqueira, Luisa Possamai Gimenes, Norberto Peporine Lopes, Luiz Alberto Lira Soares, Matheus de Freitas Fernandes-Pedrosa, Silvana Maria Zucolotto
[This corrects the article DOI: 10.1371/journal.pone.0168658.].
2017: PloS One
https://www.readbyqxmd.com/read/27975681/chemical-characterization-and-in-vitro-antibacterial-activity-of-kalanchoe-brasiliensis-cambess-crassulaceae
#4
Oas Mayorga, J R Florencio, Jdg de Santana, C Feres-Netto, D M Pedrosa, Yfg Costa, Ncc Pinto, E Scio, O V Sousa, M S Alves
No abstract text is available yet for this article.
December 2016: Planta Medica
https://www.readbyqxmd.com/read/27975578/kalanchoe-pinnata-modulates-production-of-tnf-%C3%AE-in-neutrophils
#5
F Sobreira, A R de Almeida, L da, S Borges, R F Zambonatto, F R Lourenço, E Hatanaka, E M Bacchi
No abstract text is available yet for this article.
December 2016: Planta Medica
https://www.readbyqxmd.com/read/27874020/friedelin-synthase-from-maytenus-ilicifolia-leucine-482-plays-an-essential-role-in-the-production-of-the-most-rearranged-pentacyclic-triterpene
#6
Tatiana M Souza-Moreira, Thaís B Alves, Karina A Pinheiro, Lidiane G Felippe, Gustavo M A De Lima, Tatiana F Watanabe, Cristina C Barbosa, Vânia A F F M Santos, Norberto P Lopes, Sandro R Valentini, Rafael V C Guido, Maysa Furlan, Cleslei F Zanelli
Among the biologically active triterpenes, friedelin has the most-rearranged structure produced by the oxidosqualene cyclases and is the only one containing a cetonic group. In this study, we cloned and functionally characterized friedelin synthase and one cycloartenol synthase from Maytenus ilicifolia (Celastraceae). The complete coding sequences of these 2 genes were cloned from leaf mRNA, and their functions were characterized by heterologous expression in yeast. The cycloartenol synthase sequence is very similar to other known OSCs of this type (approximately 80% identity), although the M...
November 22, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27826829/phytoextraction-of-heavy-metals-by-potential-native-plants-and-their-microscopic-observation-of-root-growing-on-stabilised-distillery-sludge-as-a-prospective-tool-for-in-situ-phytoremediation-of-industrial-waste
#7
Ram Chandra, Vineet Kumar
The safe disposal of post-methanated distillery sludge (PMDS) in the environment is challenging due to high concentrations of heavy metals along with other complex organic pollutants. The study has revealed that PMDS contained high amounts of Fe (2403), Zn (210), Mn (126), Cu (73.62), Cr (21.825), Pb (16.33) and Ni (13.425 mg kg(-1)) along with melanoidins and other co-pollutants. The phytoextraction pattern in 15 potential native plants growing on sludge showed that the Blumea lacera, Parthenium hysterophorous, Setaria viridis, Chenopodium album, Cannabis sativa, Basella alba, Tricosanthes dioica, Amaranthus spinosus L...
November 8, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27682168/35s-promoter-methylation-in-kanamycin-resistant-kalanchoe-kalanchoe-pinnata-l-plants-expressing-the-antimicrobial-peptide-cecropin-p1-transgene
#8
T V Shevchuk, N S Zakharchenko, S V Tarlachkov, O V Furs, O V Dyachenko, Y I Buryanov
Transgenic kalanchoe plants (Kalanchoe pinnata L.) expressing the antimicrobial peptide cecropin P1 gene (cecP1) under the control of the 35S cauliflower mosaic virus 35S RNA promoter and the selective neomycin phosphotransferase II (nptII) gene under the control of the nopaline synthase gene promoter were studied. The 35S promoter methylation and the cecropin P1 biosynthesis levels were compared in plants growing on media with and without kanamycin. The low level of active 35S promoter methylation further decreases upon cultivation on kanamycin-containing medium, while cecropin P1 synthesis increases...
September 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/27488985/antioxidant-efficacy-of-kalanchoe-daigremontiana-bufadienolide-rich-fraction-in-blood-plasma-in-vitro
#9
Joanna Kolodziejczyk-Czepas, Pawel Nowak, Barbara Wachowicz, Justyna Piechocka, Rafał Głowacki, Barbara Moniuszko-Szajwaj, Anna Stochmal
CONTEXT: The main source of bufadienolides is toad venom; however, plants such as members of Kalanchoe Adans. (Crassulaceae) genus may also synthesize these bioactive substances. OBJECTIVE: This is the first study on antioxidant effects and cytotoxicity of bufadienolide-rich fraction isolated from Kalanchoe daigremontiana Raym.-Hamet & H. Perrier. MATERIALS AND METHODS: The methanolic fraction was extracted from the plant roots and contained 0...
December 2016: Pharmaceutical Biology
https://www.readbyqxmd.com/read/27472283/anti-hsv-1-and-hsv-2-flavonoids-and-a-new-kaempferol-triglycoside-from-the-medicinal-plant-kalanchoe-daigremontiana
#10
Fernanda Gouvêa Gomes Ürményi, Georgia do Nascimento Saraiva, Livia Marques Casanova, Amanda Dos Santos Matos, Luiza Maria de Magalhães Camargo, Maria Teresa Villela Romanos, Sônia Soares Costa
Kalanchoe daigremontiana (Crassulaceae) is a medicinal plant native to Madagascar. The aim of this study was to investigate the flavonoid content of an aqueous leaf extract from K. daigremontiana (Kd), and assess its antiherpetic potential. The major flavonoid, kaempferol 3-O-β-d-xylopyranosyl-(1 → 2)-α-l-rhamnopyranoside (1), was isolated from the AcOEt fraction (Kd-AC). The BuOH-soluble fraction afforded quercetin 3-O-β-d-xylopyranosyl-(1 → 2)-α-l-rhamnopyranoside (2) and the new kaempferol 3-O-β-d-xylopyranosyl-(1 → 2)-α-l-rhamnopyranoside-7-O-β-d-glucopyranoside (3), named daigremontrioside...
December 2016: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/27393455/report-antimicrobial-activity-of-kalanchoe-laciniata
#11
Maria Manan, Liaqat Hussain, Hira Ijaz, Muhammad Imran Qadir
This study was conducted to identify antimicrobial potential of Kalanchoe laciniata. The plants were extracted with 30-70% aqueous-methanol and n-hexane. The antimicrobial activities were examined using agar well diffusion method against bacteria (Staphylococcus aureus, Escherichia coli) and fungi (Candidaalbicans). Results showed that E. coli were more sensitive than Staphylococcus aureus and Candida albicans. The largest zone of inhibition (52 mm) was recorded against E. coli with the n-hexane extract of Kalanchoe laciniata...
July 2016: Pakistan Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/27386095/mating-system-population-growth-and-management-scenario-for-kalanchoe-pinnata-in-an-invaded-seasonally-dry-tropical-forest
#12
Salvador González de León, Ileana Herrera, Roger Guevara
Ecological invasions are a major issue worldwide, where successful invasion depends on traits that facilitate dispersion, establishment, and population growth. The nonnative succulent plant Kalanchoe pinnata, reported as invasive in some countries, is widespread in remnants of seasonally dry tropical forest on a volcanic outcrop with high conservation value in east-central Mexico where we assessed its mating system and demographic growth and identified management strategies. To understand its local mating system, we conducted hand-pollination treatments, germination, and survival experiments...
July 2016: Ecology and Evolution
https://www.readbyqxmd.com/read/27282662/natural-remedies-used-by-bunong-people-in-mondulkiri-province-northeast-cambodia-with-special-reference-to-the-treatment-of-11-most-common-ailments
#13
François Chassagne, Sovanmoly Hul, Eric Deharo, Geneviève Bourdy
ETHNOPHARMACOLOGICAL RELEVANCE: In this paper we present a comprehensive ethnomedicinal study conducted in Mondulkiri province. Traditional knowledge about natural medicine (plants, animals, mushrooms) was investigated in Cambodia's largest indigenous community: the Bunong people. The survey aims to document the medicinal plant use of this ethnic, by focusing on the eleven most frequent diseases encountered in the area, in order to highlight species that could be recommended in public health programs...
September 15, 2016: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/27220081/bryophyllum-pinnatum-and-related-species-used-in-anthroposophic-medicine-constituents-pharmacological-activities-and-clinical-efficacy
#14
REVIEW
Karin Fürer, Ana Paula Simões-Wüst, Ursula von Mandach, Matthias Hamburger, Olivier Potterat
Bryophyllum pinnatum (syn. Kalanchoe pinnata) is a succulent perennial plant native to Madagascar that was introduced in anthroposophic medicine in the early 20th century. In recent years, we conducted a large collaborative project to provide reliable data on the chemical composition, pharmacological properties, and clinical efficacy of Bryophyllum. Here, we comprehensively review the phytochemistry, as well as the pharmacological and clinical data. As to the pharmacology, special emphasis is given to properties related to the use in anthroposophic medicine as a treatment for "hyperactivity diseases", such as preterm labor, restlessness, and sleep disorders...
July 2016: Planta Medica
https://www.readbyqxmd.com/read/27102163/potential-health-risks-posed-by-plant-derived-cumulative-neurotoxic-bufadienolides-in-south-africa
#15
Christo Botha
Bufadienolide-type cardiac glycosides have a worldwide distribution and are mainly synthesized by plants, but there are also animal sources. In South Africa, members of three genera of the Crassulaceae (Cotyledon, Tylecodon and Kalanchoe) cause a unique chronic form of cardiac glycoside poisoning, predominantly in small stock. This paretic/paralytic condition is referred to as "krimpsiekte", cotyledonosis or "nenta". "Krimpsiekte" is a plant poisoning only reported from South Africa and is regarded as the most important plant poisoning of small stock in the semi-arid Little Karoo and southern fringes of the Great Karoo...
March 16, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27034597/oxidative-stress-parameters-and-erythrocyte-membrane-adenosine-triphosphatase-activities-in-streptozotocin-induced-diabetic-rats-administered-aqueous-preparation-of-kalanchoe-pinnata-leaves
#16
Nikhil Menon, Jean Sparks, Felix O Omoruyi
BACKGROUND: Diabetes mellitus is a chronic metabolic disease that according to the World Health Organization affects more than 382 million people. The rise in diabetes mellitus coupled with the lack of an effective treatment has led many to investigate medicinal plants to identify a viable alternative. OBJECTIVE: To evaluate red blood cell (RBC) membrane adenosine triphosphatase (ATPase) activities and antioxidant levels in streptozotocin-induced diabetic rats administered aqueous preparation of Kalanchoe pinnata leaves...
April 2016: Pharmacognosy Research
https://www.readbyqxmd.com/read/26988894/chemotaxonomic-approach-to-the-central-balkan-sedum-species-based-on-distribution-of-triterpenoids-in-their-epicuticular-waxes
#17
Snežana Č Jovanović, Bojan K Zlatković, Gordana S Stojanović
Triterpenoid distribution in epicuticular waxes of 20 central Balkan Sedum L. species and four out-groups of genera: Hylotelephium H. Ohba, Crassula L., Echeveria DC., and Kalanchoe Adans. were investigated for chemotaxonomic purposes. Identification and quantification of wax triterpenoids were performed by GC/MS and GC-FID analyses. Distribution of identified triterpenoids (oleanane, lupane, and taraxerane series), as a pattern in statistical analysis, indicated very good agreement with phylogeny and systematics, except members of series Rupestria Berger, in which case the distribution of triterpenoids did not support known classification in a satisfactory manner...
April 2016: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/26927036/new-bufadienolides-isolated-from-the-roots-of-kalanchoe-daigremontiana-crassulaceae
#18
Barbara Moniuszko-Szajwaj, Łukasz Pecio, Mariusz Kowalczyk, Anna Stochmal
An aqueous extract from the roots of Kalanchoe daigremontiana turned out to be a rich source of bufadienolides. The existing literature data relate mainly to the aerial parts of Kalanchoe but there is no information about the metabolic profile of the roots, which are also used in traditional medicine. Our investigation concerning the roots of K. daigremontiana led to the isolation and characterization of eight new bufadienolides, namely 1β,3β,5β,14β,19-pentahydroxybufa-20,22-dienolide (1), 19-(acetyloxy)-1β,3β,5β,14β-tetrahydroxybufa-20,22-dienolide (2), 3β-O-α-l-rhamno-pyranosyl-5β,11α,14β,19-tetrahydroxybufa-20,22-dienolide (3), 19-(acetyloxy)-3β,5β,11α,14β-tetrahydroxybufa-20,22-dienolide (4), 3β,5β,11α,14β,19-pentahydroxy-12-oxo-bufa-20,22-dienolide (5), 19-(acetyloxy)-3β,5β,11α,14β-tetrahydroxy-12-oxo-bufa-20,22-dienolide (6), 19-(acetyloxy)-1β,3β,5β,11α,14β-pentahydroxy-12-oxo-bufa-20,22-dienolide (7) and 1β-(acetyloxy)-3β,5β,11α,14β,19-pentahydroxy-12-oxo-bufa-20,22-dienolide (8), together with seven known compounds: 11α,19-dihydroxytelocinobufagin (9), bersaldegenin-1-acetate (10), daigredorigenin-3-acetate (11), bersaldegenin-1,3,5-orthoacetate (12), bryotoxin B (13), bryophyllin B (14) and bersaldegenin (15)...
February 24, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/26909971/key-kdsoc1-gene-expression-profiles-during-plantlet-morphogenesis-under-hormone-photoperiod-and-drought-treatments
#19
C Liu, C Zhu, H M Zeng
Kalanchoe daigremontiana utilizes plantlet formation between its zigzag leaf margins as its method of asexual reproduction. In this study, K. daigremontiana SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (KdSOC1), a key intermediate in the transition from vegetative to asexual growth, was cloned. Furthermore, its expression profiles during plantlet formation under different environmental and hormone induction conditions were analyzed. The full-KdSOC1 cDNA sequence length was 1410 bp with 70% shared homology with Carya cathayensis SOC1...
February 11, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/26658852/application-of-succulent-plant-leaves-for-agrobacterium-infiltration-mediated-protein-production
#20
Richard W Jones
When expressing plant cell wall degrading enzymes in the widely used tobacco (Nicotiana benthamiana) after Agrobacterium infiltration, difficulties arise due to the thin leaf structure. Thick leaved succulents, Kalanchoe blossfeldiana and Hylotelephium telephium, were tested as alternatives. A xyloglucanase, as well as a xyloglucanase inhibitor protein was successfully produced.
January 2016: Journal of Microbiological Methods
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