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https://www.readbyqxmd.com/read/29740226/phenolic-acid-content-antioxidant-and-cytotoxic-activities-of-four-kalancho%C3%A3-species
#1
Anna Bogucka-Kocka, Christian Zidorn, Małgorzata Kasprzycka, Grażyna Szymczak, Katarzyna Szewczyk
Phenolic acid composition, antioxidant, and cytotoxic activities in leaves of four Kalanchoe (Crassulaceae) species were evaluated. Determination of phenolic acid contents were conducted by an optimized LC-ESI-MS/MS method. The results show that Kalanchoe daigremontiana Raym.-Hamet & H. Perrier (using ASE extraction) and Kalanchoe pinnata (Lam.) Pers. contain the highest amounts of phenolic acids, while Kalanchoe nyikae Engl. the lowest ones. Among phenolic acids ferulic, caffeic and protocatechuic acids were occurring in the highest quantities in the analysed species...
May 2018: Saudi Journal of Biological Sciences
https://www.readbyqxmd.com/read/29730133/cytotoxic-effect-of-kalanchoe-flammea-and-induction-of-intrinsic-mitochondrial-apoptotic-signaling-in-prostate-cancer-cells
#2
Iván Arias-González, Alejandro M García-Carrancá, Jorge Cornejo-Garrido, Cynthia Ordaz-Pichardo
Ethnopharmacological importance: Kalanchoe flammea Stapf (Crassulaceae) is a medicinal plant grown in the South of Mexico (State of Tabasco), which is commonly used in traditional medicine for the treatment of fever, wounds, inflammation, and cancer. AIM OF THE STUDY: To establish the potential of K. flammea for the treatment of prostate cancer, evaluating its cytotoxic activity, its probable mechanism of action, and carrying out some toxicological safety studies. MATERIALS AND METHODS: The cytotoxic activity of the ethyl acetate extract of K...
May 3, 2018: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29695040/gastroprotective-and-antioxidant-activity-of-kalanchoe-brasiliensis-and-kalanchoe-pinnata-leaf-juices-against-indomethacin-and-ethanol-induced-gastric-lesions-in-rats
#3
Edilane Rodrigues Dantas de Araújo, Gerlane Coelho Bernardo Guerra, Daline Fernandes de Souza Araújo, Aurigena Antunes de Araújo, Júlia Morais Fernandes, Raimundo Fernandes de Araújo Júnior, Valéria Costa da Silva, Thaís Gomes de Carvalho, Leandro de Santis Ferreira, Silvana Maria Zucolotto
Kalanchoe brasiliensis and Kalanchoe pinnata are used interchangeably in traditional medicine for treating peptic ulcers and inflammatory problems. In this context, this study aims to characterize the chemical constituents and evaluate the gastroprotective activity of the leaf juices of the two species in acute gastric lesions models. Thin Layer Chromatography (TLC) and Ultra High Performance Liquid Chromatography coupled to Mass Spectrometer (UHPLC-MS) were performed for chemical characterization. Wistar rats were pre-treated orally with leaf juices (125, 250 and 500 mg/kg) or ranitidine (50 mg/kg)...
April 24, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29593788/-in-vivo-and-in-vitro-toxicity-evaluation-of-hydroethanolic-extract-of-kalanchoe-brasiliensis-crassulaceae-leaves
#4
Aldilane Gonçalves Fonseca, Luzia Leiros Sena Fernandes Ribeiro Dantas, Júlia Morais Fernandes, Silvana Maria Zucolotto, Adley Antoninni Neves Lima, Luiz Alberto Lira Soares, Hugo Alexandre Oliveira Rocha, Telma Maria Araújo Moura Lemos
The species Kalanchoe brasiliensis , known as "Saião , " has anti-inflammatory, antimicrobial, and antihistamine activities. It also has the quercetin and kaempferol flavonoids, which exert their therapeutic activities. With extensive popular use besides the defined therapeutical properties, the study of possible side effects is indispensable. The objective of this study is to evaluate the toxicity in vitro and in vivo from the hydroethanolic extract of the leaves of K. brasiliensis . The action of the extract (concentrations from 0...
2018: Journal of Toxicology
https://www.readbyqxmd.com/read/29516033/antioxidant-activities-of-traditional-plants-in-sri-lanka-by-dpph-free-radical-scavenging-assay
#5
Kotaro Hara, Takao Someya, Katsura Sano, Yoshimasa Sagane, Toshihiro Watanabe, R G S Wijesekara
This article describes free radical-scavenging activities of extracts of several plants harvested in Sri Lanka through the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. These plants have traditionally been used in the indigenous systems of medicine in Sri Lanka, such as Ayurveda, as described below. (English name, "local name in Sri Lanka," (scientific name)). bougainvillea plant, "bouganvilla," ( Bougainvillea grabla ), purple fruited pea eggplant,"welthibbatu," ( Solanum trilobatum ) [1], country borage plant, "kapparawalliya," ( Plectranthus amboinicus ) [2], malabar nut plant, "adhatoda," ( Justicia adhatoda ) [3], long pepper plant,"thippili," ( Piper longum ) [4], holy basil plant, "maduruthala," ( Ocimum tenuiflorum ) [5], air plant, "akkapana," ( Kalanchoe pinnata ) [6], plumed cockscomb plant, "kiri-henda," ( Celosia argentea ) [7], neem plant,"kohomba," ( Azadirachta indica ) [8], balipoovu plant, "polpala," ( Aerva lanata ) [9], balloon-vine plant, "wel penera," ( Cardiospermum halicacabum ) [10], emblic myrobalan plant, "nelli," ( Phyllanthus emblica ) [11], indian copperleaf plant, "kuppameniya," ( Acalypha indica ) [12], spreading hogweed plant, "pita sudu sarana," ( Boerhavia diffusa ) [13], curry leaf plant, "karapincha," ( Murraya koenigii ) [14], indian pennywort plant, "gotukola," ( Centera asiatica ) [15], jewish plum plant, "ambarella,"( Spondias dulcis ) [16]...
April 2018: Data in Brief
https://www.readbyqxmd.com/read/29453613/in-vitro-antioxidant-and-cytotoxic-activities-of-18-plants-from-the-erkowit-region-eastern-sudan
#6
Manar Adam, Gihan O M Elhassan, Sakina Yagi, Fatma Sezer Senol, Ilkay Erdogan Orhan, Abdel Azim Ahmed, Thomas Efferth
We investigated the antioxidant potential and cytotoxicity towards human CCRF-CEM leukemia cells of 57 extracts obtained from 18 plants collected in the Erkowit region, eastern Sudan. The antioxidant activity was determined by measuring the radical scavenging effects against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and N,N-dimethyl-p-phenylendiamine (DMPD), metal-chelation capacity, ferric-reducing (FRAP) and phosphomolibdenum-reducing antioxidant power (PRAP) methods using ELISA microtiter assays. Total phenol and flavonoid amounts of the extracts were determined spectrophotometrically...
April 2018: Natural Products and Bioprospecting
https://www.readbyqxmd.com/read/29416051/a-novel-gene-of-kalanchoe-daigremontiana-confers-plant-drought-resistance
#7
Li Wang, Chen Zhu, Lin Jin, Aihua Xiao, Jie Duan, Luyi Ma
Kalanchoe (K.) daigremontiana is important for studying asexual reproduction under different environmental conditions. Here, we describe a novel KdNOVEL41 (KdN41) gene that may confer drought resistance and could thereby affect K. daigremontiana development. The detected subcellular localization of a KdN41/Yellow Fluorescent Protein (YFP) fusion protein was in the nucleus and cell membrane. Drought, salt, and heat stress treatment in tobacco plants containing the KdN41 gene promoter driving β-glucuronidase (GUS) gene transcription revealed that only drought stress triggered strong GUS staining in the vascular tissues...
February 7, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29349835/optimization-of-aqueous-extraction-from-kalanchoe-pinnata-leaves-to-obtain-the-highest-content-of-an-anti-inflammatory-flavonoid-using-a-response-surface-model
#8
Luana Beatriz Dos Santos Nascimento, Paula Fernandes de Aguiar, Marcos Vinicius Leal-Costa, Marcela Araújo Soares Coutinho, Maria Paula Gonçalves Borsodi, Bartira Rossi-Bergmann, Eliana Schwartz Tavares, Sônia Soares Costa
INTRODUCTION: The medicinal plant Kalanchoe pinnata is a phenolic-rich species used worldwide. The reports on its pharmacological uses have increased by 70% in the last 10 years. The leaves of this plant are the main source of an unusual quercetin-diglycosyl flavonoid (QAR, quercetin arabinopyranosyl rhamnopyranoside), which can be easily extracted using water. QAR possess a strong in vivo anti-inflammatory activity. OBJECTIVE: To optimize the aqueous extraction of QAR from K...
May 2018: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/29200987/bufadienolides-of-kalanchoe-species-an-overview-of-chemical-structure-biological-activity-and-prospects-for-pharmacological-use
#9
REVIEW
Joanna Kolodziejczyk-Czepas, Anna Stochmal
Toad venom is regarded as the main source of bufadienolides; however, synthesis of these substances takes also place in a variety of other animal and plant organisms, including ethnomedicinal plants of the Kalanchoe genus. Chemically, bufadienolides are a group of polyhydroxy C-24 steroids and their glycosides, containing a six-membered lactone (α-pyrone) ring at the C-17β position. From the pharmacological point of view, bufadienolides might be a promising group of steroid hormones with cardioactive properties and anticancer activity...
2017: Phytochemistry Reviews: Proceedings of the Phytochemical Society of Europe
https://www.readbyqxmd.com/read/29196618/the-kalancho%C3%A3-genome-provides-insights-into-convergent-evolution-and-building-blocks-of-crassulacean-acid-metabolism
#10
Xiaohan Yang, Rongbin Hu, Hengfu Yin, Jerry Jenkins, Shengqiang Shu, Haibao Tang, Degao Liu, Deborah A Weighill, Won Cheol Yim, Jungmin Ha, Karolina Heyduk, David M Goodstein, Hao-Bo Guo, Robert C Moseley, Elisabeth Fitzek, Sara Jawdy, Zhihao Zhang, Meng Xie, James Hartwell, Jane Grimwood, Paul E Abraham, Ritesh Mewalal, Juan D Beltrán, Susanna F Boxall, Louisa V Dever, Kaitlin J Palla, Rebecca Albion, Travis Garcia, Jesse A Mayer, Sung Don Lim, Ching Man Wai, Paul Peluso, Robert Van Buren, Henrique Cestari De Paoli, Anne M Borland, Hong Guo, Jin-Gui Chen, Wellington Muchero, Yanbin Yin, Daniel A Jacobson, Timothy J Tschaplinski, Robert L Hettich, Ray Ming, Klaus Winter, James H Leebens-Mack, J Andrew C Smith, John C Cushman, Jeremy Schmutz, Gerald A Tuskan
Crassulacean acid metabolism (CAM) is a water-use efficient adaptation of photosynthesis that has evolved independently many times in diverse lineages of flowering plants. We hypothesize that convergent evolution of protein sequence and temporal gene expression underpins the independent emergences of CAM from C3 photosynthesis. To test this hypothesis, we generate a de novo genome assembly and genome-wide transcript expression data for Kalanchoë fedtschenkoi, an obligate CAM species within the core eudicots with a relatively small genome (~260 Mb)...
December 1, 2017: Nature Communications
https://www.readbyqxmd.com/read/29148773/in-situ-nondestructive-analysis-of-kalanchoe-pinnata-leaf-surface-structure-by-polarization-modulation-infrared-reflection-absorption-spectroscopy
#11
Tetsuya Hama, Akira Kouchi, Naoki Watanabe, Shinichi Enami, Takafumi Shimoaka, Takeshi Hasegawa
The outermost surface of the leaves of land plants is covered with a lipid membrane called the cuticle that protects against various stress factors. Probing the molecular-level structure of the intact cuticle is highly desirable for understanding its multifunctional properties. We report the in situ characterization of the surface structure of Kalanchoe pinnata leaves using polarization-modulation infrared reflection-absorption spectroscopy (PM-IRRAS). Without sample pretreatment, PM-IRRAS measures the IR spectra of the leaf cuticle of a potted K...
December 14, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/29147934/an-outbreak-of-poisoning-by-kalanchoe-blossfeldiana-in-cattle-in-northeastern-brazil
#12
Fábio S Mendonça, Naiara C F Nascimento, Valdir M Almeida, Thaiza C Braga, Daniele P Ribeiro, Hisadora A S Chaves, Givaldo B Silva Filho, Franklin Riet-Correa
In the world, Kalanchoe species are primarily ornamentals and houseplants, but some have escaped cultivation and can be found in the field. In Latin America, there are no reports of spontaneous poisoning by Kalanchoe species in animals. This study aimed to describe the epidemiological, clinical, and pathological aspects of an outbreak of poisoning by Kalanchoe blossfeldiana in cattle in the semiarid region of Pernambuco, Brazil. Epidemiological and clinical data were obtained from the owner and veterinarian during technical visits...
March 2018: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28917388/improvised-hand-injury-treatment-using-traditional-veterinary-medicine-in-ethiopia
#13
Raf Aerts, Eva J J November, Maissa Rayyan
In remote wilderness environments, local people with traditional knowledge of medicinal plants are potentially important first-line health care providers. We present a case of a 31-year-old man who fell off a horse while trekking through a remote mountain landscape in Ethiopia and sustained blunt force trauma to the hand. A local mountain hut keeper examined the patient's hand and used heated leaves of the succulent plant Kalanchoe petitiana to treat a suspected metacarpal fracture. As first responder in a low-resource setting, the hut keeper relied on his traditional knowledge of ethnoveterinary medicine to improvise a treatment for a human injury in a remote mountain environment...
December 2017: Wilderness & Environmental Medicine
https://www.readbyqxmd.com/read/28887405/phosphorylation-of-phospho-enol-pyruvate-carboxylase-is-essential-for-maximal-and-sustained-dark-co-2-fixation-and-core-circadian-clock-operation-in-the-obligate-crassulacean-acid-metabolism-species-kalancho%C3%A3-fedtschenkoi
#14
Susanna F Boxall, Louisa V Dever, Jana Kneřová, Peter D Gould, James Hartwell
Phospho enol pyruvate carboxylase (PPC; EC 4.1.1.31) catalyzes primary nocturnal CO2 fixation in Crassulacean acid metabolism (CAM) species. CAM PPC is regulated posttranslationally by a circadian clock-controlled protein kinase called phospho enol pyruvate carboxylase kinase (PPCK). PPCK phosphorylates PPC during the dark period, reducing its sensitivity to feedback inhibition by malate and thus enhancing nocturnal CO2 fixation to stored malate. Here, we report the generation and characterization of transgenic RNAi lines of the obligate CAM species Kalanchoë fedtschenkoi with reduced levels of KfPPCK1 transcripts...
October 2017: Plant Cell
https://www.readbyqxmd.com/read/28717174/over-expression-of-kdsoc1-gene-affected-plantlet-morphogenesis-in-kalanchoe-daigremontiana
#15
Chen Zhu, Li Wang, Jinhua Chen, Chenglan Liu, Huiming Zeng, Huafang Wang
Kalanchoe daigremontiana reproduces asexually by producing plantlets along the leaf margin. The aim of this study was to identify the function of the SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 gene in Kalanchoe daigremontiana (KdSOC1) during plantlet morphogenesis. In this study, KdSOC1 gene expression was detected at stem cell niche during in vitro somatic embryogenesis and plantlet morphogenesis. Disrupting endogenous auxin transportation suppressed the KdSOC1 gene response. Knockdown of the KdSOC1 gene caused a defect in cotyledon formation during the early heart stage of somatic embryogenesis...
July 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28694760/genotoxic-and-cytotoxic-potential-of-whole-plant-extracts-of-kalanchoe-laciniata-by-ames-and-mtt-assay
#16
Ali Sharif, Muhammad Furqan Akhtar, Bushra Akhtar, Ammara Saleem, Maria Manan, Maryam Shabbir, Muneeb Ashraf, Sohaib Peerzada, Shoaib Ahmed, Moosa Raza
Lack of data on safety of herbal medicines have endangered human health and life. The present study evaluated the genotoxic and mutagenic effect of Kalanchoe laciniata to access the safety and usefulness of the medicinal plant. Aqua-methanolic and n-hexane extracts of K. laciniata were evaluated for the genotoxic potential using Ames assay and cytotoxicity was evaluated using MTT assay. Ames assay was conducted using two strains of Salmonella typhimurium TA-100 and TA-102 whereas MTT assay was performed on baby hamster kidney cell line BHK-21...
2017: EXCLI Journal
https://www.readbyqxmd.com/read/28545532/cytotoxicity-of-the-methanol-extracts-of-elephantopus-mollis-kalanchoe-crenata-and-4-other-cameroonian-medicinal-plants-towards-human-carcinoma-cells
#17
Victor Kuete, Fabrice W Fokou, Oğuzhan Karaosmanoğlu, Veronique P Beng, Hülya Sivas
BACKGROUND: Cancer still constitutes one of the major health concerns globally, causing serious threats on patients, their families, and the healthcare system. METHODS: In this study, the cytotoxicity of the methanol extract of Elephantopus mollis whole plant (EMW), Enantia chlorantha bark (ECB), Kalanchoe crenata leaves (KCL), Lophira alata bark (LAB), Millettia macrophylla leaves (MML) and Phragmanthera capitata leaves (PCL) towards five human solid cancer cell lines and normal CRL2120 fibroblasts, was evaluated...
May 25, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28395583/isolation-and-identification-of-compounds-from-kalanchoe-pinnata-having-human-alphaherpesvirus-and-vaccinia-virus-antiviral-activity
#18
Matthew Cryer, Kyle Lane, Mary Greer, Rex Cates, Scott Burt, Merritt Andrus, Jiping Zou, Paul Rogers, Marc D H Hansen, Jillybeth Burgado, Subbian Satheshkumar Panayampalli, Craig W Day, Donald F Smee, Brent F Johnson
CONTEXT: Kalanchoe pinnata (Lam.) Pers. (Crassulaceae) is a succulent plant that is known for its traditional antivirus and antibacterial usage. OBJECTIVE: This work examines two compounds identified from the K. pinnata plant for their antivirus activity against human alphaherpesvirus (HHV) 1 and 2 and vaccinia virus (VACV). MATERIALS AND METHODS: Compounds KPB-100 and KPB-200 were isolated using HPLC and were identified using NMR and MS. Both compounds were tested in plaque reduction assay of HHV-2 wild type (WT) and VACV...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28393374/nitrate-enhancement-of-cam-activity-in-two-kalancho%C3%A3-species-is-associated-with-increased-vacuolar-proton-transport-capacity
#19
Paula Natália Pereira, James Andrew Charles Smith, Helenice Mercier
Among species that perform CAM photosynthesis, members of the genus Kalanchoë have been studied frequently to investigate the effect of environmental factors on the magnitude of CAM activity. In particular, different nitrogen sources have been shown to influence the rate of nocturnal CO2 fixation and organic-acid accumulation in several species of Kalanchoë. However, there has been little investigation of the interrelationship between nitrogen source (nitrate versus ammonium), concentration and the activity of the vacuolar proton pumps responsible for driving nocturnal organic-acid accumulation in these species...
August 2017: Physiologia Plantarum
https://www.readbyqxmd.com/read/28326334/bactericide-immunomodulating-and-wound-healing-properties-of-transgenic-kalanchoe-pinnata-synergize-with-antimicrobial-peptide-cecropin-p1-in-vivo
#20
COMPARATIVE STUDY
A A Lebedeva, N S Zakharchenko, E V Trubnikova, O A Medvedeva, T V Kuznetsova, G A Masgutova, M V Zylkova, Y I Buryanov, A S Belous
Procedure of manufacturing K. pinnata water extracts containing cecropin P1 (CecP1) from the formerly described transgenic plants is established. It included incubation of leaves at +4°C for 7 days, mechanical homogenization of leaves using water as extraction solvent, and heating at +70°C for inactivating plant enzymes. Yield of CecP1 (after heating and sterilizing filtration) was 0.3% of total protein in the extract. The water extract of K. pinnata + CecP1 exhibits favorable effect on healing of wounds infected with S...
2017: Journal of Immunology Research
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