Read by QxMD icon Read

food specific immunoglobulin G antibodies

Thomas B Martins, Michael E Bandhauer, Diane M Wilcock, Harry R Hill, Patricia R Slev
BACKGROUND: The clinical utility of serum IgG measurement in the diagnosis of allergy and food-induced hypersensitivity has been largely discredited. Recent studies, however, have shown that specific IgG can inhibit IgE mediated allergies, and may play a role in allergen specific desensitization. Accurate reference intervals for IgG specific allergens have not been widely established and are needed for better interpretation of serum antibody concentrations. In this study we established 64 IgG reference intervals for 48 common food allergens, 5 venoms, and 11 molds...
December 2016: Annals of Clinical and Laboratory Science
Jacek Gocki, Zbigniew Bartuzi
This paper presents current views on the role of immunoglobulin G (IgG) antibodies in the reactions with food antigens in the digestive tract and their role in the diagnosis of food allergy based on the assays of specific IgG class antibodies, with a special focus on contemporary practice guidelines. In the light of current scientific knowledge, the IgG-specific antibody-mediated reactions are a body's natural and normal defensive reactions to infiltrating food antigens, which are considered as pathogens. On the other hand, specific IgG antibodies against food allergens play a crucial role in the induction and maintaining of immunological tolerance to food antigens...
August 2016: Postȩpy Dermatologii i Alergologii
Xinjie Song, Shruti Shukla, Gibaek Lee, Sunhyun Park, Myunghee Kim
Cronobacter species (Cronobacter spp.) are hazardous foodborne pathogens associated with baby food, powdered infant formula (PIF). To develop a rapid and sensitive method for simultaneous detection of seven Cronobacter spp. in PIF, an indirect non-competitive enzyme-linked immunosorbent assay (INC-ELISA) was developed based on a novel immunoglobulin G (IgG), anti-Cronobacter IgG. The developed INC-ELISA was able to detect seven Cronobacter spp. at concentrations ranging from (5.6 ± 0.30) × 10(3) to (2.1 ± 0...
2016: Frontiers in Microbiology
Hanqing He, Pingping Yao, Yang Zhou, Xuan Deng, Jinren Pan
BACKGROUND: The resurgence of pertussis has occurred in many countries. However, the epidemiological profiles of pertussis cannot be well understood by the current surveillance system in China. This study was designed to investigate the age specific serologic evidence of antibodies against pertussis, and to offer information regarding the existence of pertussis infection in Zhejiang Province, China. METHODS: A cross-sectional serosurvey was carried out in 6 counties of Zhejiang Province during September and October of 2014...
2016: PloS One
Jianjie Li, Hao Yan, He Chen, Qiongmei Ji, Shengguang Huang, Pingchang Yang, Zhigang Liu, Bo Yang
BACKGROUND: Rheumatoid arthritis (RA) is a very complicated autoimmune disease with apparent synovial hyperplasia and cartilage and bone destruction. AIMS: In the present study, we aimed to determine whether the pathogenesis of RA correlates with food allergy and which allergen(s) are relevant. MATERIALS AND METHODS: We used type-II collagen (CII) to induce arthritis (collagen-induced arthritis, CIA) model in Wistar rats, and the development of arthritis was evaluated accordingly by scoring system...
January 2016: North American Journal of Medical Sciences
P Xepapadaki, A Fiocchi, L Grabenhenrich, G Roberts, K E C Grimshaw, A Fiandor, J I Larco, S Sigurdardottir, M Clausen, N G Papadopoulos, L Dahdah, A Mackie, A B Sprikkelman, A A Schoemaker, R Dubakiene, I Butiene, M L Kowalski, K Zeman, S Gavrili, T Keil, K Beyer
BACKGROUND: Parents and health staff perceive hen's egg allergy (HEA) as a common food allergy in early childhood, but the true incidence is unclear because population-based studies with gold-standard diagnostic criteria are lacking. OBJECTIVE: To establish the incidence and course of challenge-confirmed HEA in children, from birth until the age of 24 months, in different European regions. METHODS: In the EuroPrevall birth cohort study, children with a suspected HEA and their age-matched controls were evaluated in 9 countries, using a standardized protocol including measurement of HE-specific immunoglobulin E-antibodies in serum, skin prick tests, and double-blind, placebo-controlled food challenges (DBPCFC)...
March 2016: Allergy
Robert G Hamilton, Joerg Kleine-Tebbe
Application of purified native and recombinant allergenic molecules into IgE antibody assays can improve analytical sensitivity and specificity for selected allergen specificities. They enhance analytical sensitivity by allowing assays to detect IgE antibodies with a lower limit of quantification (LoQ) to missing or poorly represented allergens in diagnostic extracts that are commonly used in vivo and in vitro. Use of selected allergenic molecules can help improve the clinician's prediction of the risk of a serious allergic reaction to stable allergens...
September 2015: Current Allergy and Asthma Reports
Ondulla T Toomer, Andrew B Do, Tong J Fu, Kristina M Williams
UNLABELLED: Shellfish allergy affects 2% of the adult population in the United States. Identification of allergenic shrimp proteins is needed for improved management and assessment of shrimp allergy. We determined the temporal pepsin and pancreatin stability of total shrimp proteins using simulated physiological digestive conditions in vitro. Gel electrophoresis was used to determine protein stability, whereas immunoreactivity of protease stable proteins was determined using rabbit antigen-specific antibodies...
July 2015: Journal of Food Science
Michaela Čadková, Radovan Metelka, Lucie Holubová, Daniel Horák, Veronika Dvořáková, Zuzana Bílková, Lucie Korecká
Screen-printed platinum electrodes as transducer and magnetic beads as solid phase were combined to develop a particle-based electrochemical immunosensor for monitoring the serious food allergen ovalbumin. The standard arrangement of enzyme-linked immunosorbent assay became the basis for designing the immunosensor. A sandwich-type immunocomplex was formed between magnetic particles functionalized with specific anti-ovalbumin immunoglobulin G and captured ovalbumin molecules, and secondary anti-ovalbumin antibodies conjugated with the enzyme horseradish peroxidase were subsequently added as label tag...
September 1, 2015: Analytical Biochemistry
Ronald van Ree, Lars K Poulsen, Gary Wk Wong, Barbara K Ballmer-Weber, Zhongshan Gao, Xudong Jia
Food allergy is phenotypically an extremely heterogeneous group of diseases affecting multiple organs, sometimes in an isolated way, sometimes simultaneously, with the severity of reactions ranging from mild and local to full-blown anaphylaxis. Mechanistically, it is defined as a Th2-driven immune disorder in which food-specific IgE antibodies are at the basis of immediate-type adverse reactions. The sites of sensitization and symptoms do not necessarily overlap. Food allergy, which is the theme of this paper, is often confused with other adverse reactions to food of both animmune (e...
January 2015: Zhonghua Yu Fang Yi Xue za Zhi [Chinese Journal of Preventive Medicine]
T B Sentsova, I V Vorozhko, V A Isakov, S V Morozov, A K Shakhovskaia
OBJECTIVE: To develop an algorithm for evaluation of the immune status in IBS patients with food intolerance. MATERIALS AND METHODS: 42 patients with IBS were observed. The diagnosis is based Rome III criteria. Determination of the concentration of serum allergen-IgG-antibodies to food allergens, IgG, IgA, IgM, IgE and immunoregulatory substances IL-5, TGF β1, IL-10, IL-4 IL-2, IL-13 were determined by enzyme immunoassay. All the patients received rotational diet therapy based on immunoassay data in addition to medical treatment of the basis disease...
2014: Experimental & Clinical Gastroenterology
Astrid Hogenkamp, Leon M J Knippels, Johan Garssen, Betty C A M van Esch
BACKGROUND: The maternal environment and early life exposure affect immune development in offspring. OBJECTIVE: We investigated whether development of food allergy in offspring is affected by supplementing pregnant or lactating sensitized or nonsensitized mice with a mixture of nondigestible oligosaccharides. METHODS: Dams were sensitized intragastrically with ovalbumin before mating, with use of cholera toxin (CT) as an adjuvant. Nonsensitized dams received CT only...
May 2015: Journal of Nutrition
Yutaka Shimizu, Hiroshi Oda, Kohsuke Seiki, Hiroki Saeki
A novel enzyme-linked immunosorbent assay (ELISA) system has been established for selective detection of chum salmon (Oncorhynchus keta) yolk protein (SYP). Rabbit and rat polyclonal Immunoglobulin G antibodies to β'-component (the major allergic protein in fish roe; anti-β) were applied for designing the ELISA system. The sandwich ELISA using rabbit anti-β for the capture antibody and horseradish peroxidase-labeled F(ab')2 fragment of rat anti-β for the detection antibody obtained high sensitivity and narrow specificity for SYP...
August 15, 2015: Food Chemistry
Chad J Roy, Robert N Brey, Nicholas J Mantis, Kelly Mapes, Iliodora V Pop, Laurentiu M Pop, Stephen Ruback, Stephanie Z Killeen, Lara Doyle-Meyers, Heather S Vinet-Oliphant, Peter J Didier, Ellen S Vitetta
Ricin toxin (RT) is the second most lethal toxin known; it has been designated by the CDC as a select agent. RT is made by the castor bean plant; an estimated 50,000 tons of RT are produced annually as a by-product of castor oil. RT has two subunits, a ribotoxic A chain (RTA) and galactose-binding B chain (RTB). RT binds to all mammalian cells and once internalized, a single RTA catalytically inactivates all of the ribosomes in a cell. Administered as an aerosol, RT causes rapid lung damage and fibrosis followed by death...
March 24, 2015: Proceedings of the National Academy of Sciences of the United States of America
Mirko Faber, Susanne Schink, Anne Mayer-Scholl, Christoph Ziesch, Ralph Schönfelder, Heidi Wichmann-Schauer, Klaus Stark, Karsten Nöckler
BACKGROUND: Food safety authorities discovered that wild boar meat products contaminated with Trichinella spiralis had entered the food chain in Germany in March 2013. Public health authorities issued guidelines for health professionals including post-exposure prophylaxis (PEP) using mebendazole and advised the public to seek medical advice if exposed. Our objective was to identify factors associated with the development of trichinellosis and to evaluate post exposure prophylaxis. METHODS: Persons who reported to local public health departments as exposed were interviewed concerning exposure, symptoms, and medication...
June 15, 2015: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
Cherie M Pucheu-Haston, Petra Bizikova, Melissa N C Eisenschenk, Domenico Santoro, Tim Nuttall, Rosanna Marsella
BACKGROUND: Canine atopic dermatitis (AD) is considered to be an immunoglobulin E (IgE)-mediated hypersensitivity response to environmental allergens. The role of other antibody isotypes and nonenvironmental allergens in disease pathogenesis remains unclear. OBJECTIVES: The objective of this review is to provide an update on advances in the understanding of the relevance of specific antibody isotypes, autoallergens and nonenvironmental allergens in the pathogenesis of canine AD...
April 2015: Veterinary Dermatology
Kulveen Virdee, Jeannette Musset, Matthew Baral, Courtney Cronin, Jeffrey Langland
Asthma is one of the most common causes of office visits in the primary care and emergency care settings. Individuals are often able to maintain symptomatic control with long-term pharmacological therapy. Exacerbations of asthma commonly occur due to exposure to triggers such as viruses, pollutants, and allergens. While it is widely accepted that exposure to immunoglobulin E food allergens can exacerbate asthma symptoms, there is little evidence examining delayed immunoglobulin G-mediated reactions to food...
January 2015: Global Advances in Health and Medicine: Improving Healthcare Outcomes Worldwide
S Salamati, C Martins, B Kulseng
Baker's yeast (Saccharomyces cerevisiae) and its cell wall components have been used as one of the alternatives to antibiotic growth promoters in the feed industry. Antibodies to cell wall mannan of this yeast (ASCA) have been traditionally used in the study of Crohn's disease (CD). We applied ASCA in relation to obesity. This study aims (i) to determine the concentration of ASCA (immunoglobulin A [IgA] and immunoglobulin G [IgG]) in obese compared with normal weight individuals and (ii) to determine if there is a correlation between ASCA concentrations, obesity indices and C-reactive protein...
February 2015: Clinical Obesity
Aristo Vojdani
CONTEXT: Certain individuals are sensitive enough to react to peanuts and peanut oil, sometimes with deadly effect. It is thus crucial to have an accurate testing methodology for the assessment of allergies and immune reactivities to peanuts and their components, such as agglutinins and oleosins. Currently, skin-prick testing is performed only with the water-soluble components of peanut proteins and can produce false negatives. Testing with all possible food antigens and with both immunoglobulin G (IgG) and immunoglobulin E (IgE) antibodies may offer a more accurate alternative...
2015: Alternative Therapies in Health and Medicine
Aristo Vojdani
Lectins are carbohydrate-binding proteins present throughout nature that act as agglutinins. Approximately 30% of our food contains lectins, some of which may be resistant enough to digestion to enter the circulation. Because of their binding properties, lectins can cause nutrient deficiencies, disrupt digestion, and cause severe intestinal damage when consumed in excess by an individual with dysfunctional enzymes. These effects are followed by disruption of intestinal barrier integrity, which is the gateway to various autoimmunities...
2015: Alternative Therapies in Health and Medicine
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"