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food specific immunoglobulin G antibodies

Cecilia Lourdudoss, Sofia Ajeganova, Johan Frostegård
OBJECTIVES: Immunoglobulin M (IgM) antibodies against phosphorylcholine (anti-PC) and malondialdehyde (anti-MDA) are implicated in systemic lupus erythematosus (SLE) as markers with potential protective properties. Low IgM anti-PC levels are more common in SLE than in control population. Little is known what influences the levels of these antibodies. We here studied associations between dietary and metabolic factors and levels of IgM anti-PC and anti-MDA. METHODS: This study included 109 SLE patients and 106 controls from SLE Vascular Investigation Cohort (SLEVIC)...
May 8, 2018: Clinical and Experimental Rheumatology
Baifen Song, Hongyu Cui, Longzhu Ju, Li Song, Lijie Tang, Yijing Li
We previously developed a stable and marker-free Lactobacillus casei strain (PPαT Δupp) that contained a chromosomally integrated expression cassette (PPαT) that enabled the surface expression of the Clostridium perfringens alpha toxin. To measure immune responses against the alpha toxin, specific-pathogen-free BALB/c mice were inoculated with L. casei PPαT Δupp by oral gavage. Then, specific immunoglobulin A (IgA) and immunoglobulin G (IgG) antibodies and cytokines were measured by enzyme-linked immunosorbent assay (ELISA) and flow cytometry (FCM)...
March 7, 2018: Microbial Pathogenesis
Camille Martin, Claire Kizlik-Masson, André Pèlegrin, Hervé Watier, Marie-Claude Viaud-Massuard, Nicolas Joubert
The annual "Antibody Industrial Symposium", co organized by LabEx MAbImprove, MabDesign and Polepharma, was held in Tours, France on June 27-28, 2017. The focus was on antibody-drug-conjugates (ADCs), new entities which realize the hope of Paul Ehrlich's magic bullet. ADCs result from the bioconjugation of a highly cytotoxic drug to a selective monoclonal antibody, which acts as a vector. Building on knowledge gained during the development of three approved ADCs, brentuximab vedotin (Adcetris®), ado trastuzumab emtansine (Kadcyla®) and inotuzumab ozogamicin (Besponsa®), and the many ADCs in development, this meeting addressed strategies and the latest innovations in the field from fundamental research to manufacturing...
February 2018: MAbs
Przemyslaw Zdziarski, Andrzej Gamian, Jacek Majda, Agnieszka Korzeniowska-Kowal
BACKGROUND: Allergic, especially anaphylactic, reactions during immunoglobulin replacement therapy are rare, but their pathophysiology and classification remain ambiguous. Recent findings show positive results of skin tests with commercially available immunoglobulins, but target antigens and responsible compounds of the tested immunoglobulins have not been strictly identified. CASE DESCRIPTION AND FINDINGS: Four adult patients with recently diagnosed common variable immunodeficiency qualified for standard subcutaneous immunoglobulin replacement therapy regimen...
2017: Allergy, Asthma, and Clinical Immunology
Roberto Papini, Pierluigi di Ciccio, Marianna Marangi, Sergio Ghidini, Emanuela Zanardi, Alberto Vergara, Annunziata Giangaspero, Simona Nardoni, Guido Rocchigiani, Francesca Mancianti, Adriana Ianieri
To evaluate the occurrence of Toxoplasma gondii and to genetically characterize its isolates in carcasses of industrial fattening pigs, blood, diaphragm, and heart samples were collected from 375 carcasses of pigs slaughtered to be processed for Parma ham production. Pigs had been bred on approved farms (n = 75) located in the so-called Food Valley in Italy. Sera were examined for immunoglobulin G antibodies to T. gondii by modified agglutination test (MAT). Both heart and diaphragm samples from seropositive carcasses were processed for the presence of T...
March 2017: Journal of Food Protection
Laura K Allred, Katherine Lesko, Diane McKiernan, Cynthia Kupper, Stefano Guandalini
Enzymatic digestion, or hydrolysis, has been proposed for treating gluten-containing foods and beverages to make them safe for persons with celiac disease (CD). There are no validated testing methods that allow the quantitation of all the hydrolyzed or fermented gluten peptides in foods and beverages that might be harmful to CD patients, making it difficult to assess the safety of hydrolyzed products. This study examines an ELISA-based method to determine whether serum antibody binding of residual peptides in a fermented barley-based product is greater among active-CD patients than a normal control group, using commercial beers as a test case...
March 1, 2017: Journal of AOAC International
Thomas B Martins, Michael E Bandhauer, Diane M Wilcock, Harry R Hill, Patricia R Slev
BACKGROUND: The clinical utility of serum IgG measurement in the diagnosis of allergy and food-induced hypersensitivity has been largely discredited. Recent studies, however, have shown that specific IgG can inhibit IgE mediated allergies, and may play a role in allergen specific desensitization. Accurate reference intervals for IgG specific allergens have not been widely established and are needed for better interpretation of serum antibody concentrations. In this study we established 64 IgG reference intervals for 48 common food allergens, 5 venoms, and 11 molds...
December 2016: Annals of Clinical and Laboratory Science
Jacek Gocki, Zbigniew Bartuzi
This paper presents current views on the role of immunoglobulin G (IgG) antibodies in the reactions with food antigens in the digestive tract and their role in the diagnosis of food allergy based on the assays of specific IgG class antibodies, with a special focus on contemporary practice guidelines. In the light of current scientific knowledge, the IgG-specific antibody-mediated reactions are a body's natural and normal defensive reactions to infiltrating food antigens, which are considered as pathogens. On the other hand, specific IgG antibodies against food allergens play a crucial role in the induction and maintaining of immunological tolerance to food antigens...
August 2016: Postȩpy Dermatologii i Alergologii
Xinjie Song, Shruti Shukla, Gibaek Lee, Sunhyun Park, Myunghee Kim
Cronobacter species (Cronobacter spp.) are hazardous foodborne pathogens associated with baby food, powdered infant formula (PIF). To develop a rapid and sensitive method for simultaneous detection of seven Cronobacter spp. in PIF, an indirect non-competitive enzyme-linked immunosorbent assay (INC-ELISA) was developed based on a novel immunoglobulin G (IgG), anti-Cronobacter IgG. The developed INC-ELISA was able to detect seven Cronobacter spp. at concentrations ranging from (5.6 ± 0.30) × 10(3) to (2.1 ± 0...
2016: Frontiers in Microbiology
Hanqing He, Pingping Yao, Yang Zhou, Xuan Deng, Jinren Pan
BACKGROUND: The resurgence of pertussis has occurred in many countries. However, the epidemiological profiles of pertussis cannot be well understood by the current surveillance system in China. This study was designed to investigate the age specific serologic evidence of antibodies against pertussis, and to offer information regarding the existence of pertussis infection in Zhejiang Province, China. METHODS: A cross-sectional serosurvey was carried out in 6 counties of Zhejiang Province during September and October of 2014...
2016: PloS One
Jianjie Li, Hao Yan, He Chen, Qiongmei Ji, Shengguang Huang, Pingchang Yang, Zhigang Liu, Bo Yang
BACKGROUND: Rheumatoid arthritis (RA) is a very complicated autoimmune disease with apparent synovial hyperplasia and cartilage and bone destruction. AIMS: In the present study, we aimed to determine whether the pathogenesis of RA correlates with food allergy and which allergen(s) are relevant. MATERIALS AND METHODS: We used type-II collagen (CII) to induce arthritis (collagen-induced arthritis, CIA) model in Wistar rats, and the development of arthritis was evaluated accordingly by scoring system...
January 2016: North American Journal of Medical Sciences
P Xepapadaki, A Fiocchi, L Grabenhenrich, G Roberts, K E C Grimshaw, A Fiandor, J I Larco, S Sigurdardottir, M Clausen, N G Papadopoulos, L Dahdah, A Mackie, A B Sprikkelman, A A Schoemaker, R Dubakiene, I Butiene, M L Kowalski, K Zeman, S Gavrili, T Keil, K Beyer
BACKGROUND: Parents and health staff perceive hen's egg allergy (HEA) as a common food allergy in early childhood, but the true incidence is unclear because population-based studies with gold-standard diagnostic criteria are lacking. OBJECTIVE: To establish the incidence and course of challenge-confirmed HEA in children, from birth until the age of 24 months, in different European regions. METHODS: In the EuroPrevall birth cohort study, children with a suspected HEA and their age-matched controls were evaluated in 9 countries, using a standardized protocol including measurement of HE-specific immunoglobulin E-antibodies in serum, skin prick tests, and double-blind, placebo-controlled food challenges (DBPCFC)...
March 2016: Allergy
Robert G Hamilton, Joerg Kleine-Tebbe
Application of purified native and recombinant allergenic molecules into IgE antibody assays can improve analytical sensitivity and specificity for selected allergen specificities. They enhance analytical sensitivity by allowing assays to detect IgE antibodies with a lower limit of quantification (LoQ) to missing or poorly represented allergens in diagnostic extracts that are commonly used in vivo and in vitro. Use of selected allergenic molecules can help improve the clinician's prediction of the risk of a serious allergic reaction to stable allergens...
September 2015: Current Allergy and Asthma Reports
Ondulla T Toomer, Andrew B Do, Tong J Fu, Kristina M Williams
UNLABELLED: Shellfish allergy affects 2% of the adult population in the United States. Identification of allergenic shrimp proteins is needed for improved management and assessment of shrimp allergy. We determined the temporal pepsin and pancreatin stability of total shrimp proteins using simulated physiological digestive conditions in vitro. Gel electrophoresis was used to determine protein stability, whereas immunoreactivity of protease stable proteins was determined using rabbit antigen-specific antibodies...
July 2015: Journal of Food Science
Michaela Čadková, Radovan Metelka, Lucie Holubová, Daniel Horák, Veronika Dvořáková, Zuzana Bílková, Lucie Korecká
Screen-printed platinum electrodes as transducer and magnetic beads as solid phase were combined to develop a particle-based electrochemical immunosensor for monitoring the serious food allergen ovalbumin. The standard arrangement of enzyme-linked immunosorbent assay became the basis for designing the immunosensor. A sandwich-type immunocomplex was formed between magnetic particles functionalized with specific anti-ovalbumin immunoglobulin G and captured ovalbumin molecules, and secondary anti-ovalbumin antibodies conjugated with the enzyme horseradish peroxidase were subsequently added as label tag...
September 1, 2015: Analytical Biochemistry
Ronald van Ree, Lars K Poulsen, Gary Wk Wong, Barbara K Ballmer-Weber, Zhongshan Gao, Xudong Jia
Food allergy is phenotypically an extremely heterogeneous group of diseases affecting multiple organs, sometimes in an isolated way, sometimes simultaneously, with the severity of reactions ranging from mild and local to full-blown anaphylaxis. Mechanistically, it is defined as a Th2-driven immune disorder in which food-specific IgE antibodies are at the basis of immediate-type adverse reactions. The sites of sensitization and symptoms do not necessarily overlap. Food allergy, which is the theme of this paper, is often confused with other adverse reactions to food of both animmune (e...
January 2015: Zhonghua Yu Fang Yi Xue za Zhi [Chinese Journal of Preventive Medicine]
T B Sentsova, I V Vorozhko, V A Isakov, S V Morozov, A K Shakhovskaia
OBJECTIVE: To develop an algorithm for evaluation of the immune status in IBS patients with food intolerance. MATERIALS AND METHODS: 42 patients with IBS were observed. The diagnosis is based Rome III criteria. Determination of the concentration of serum allergen-IgG-antibodies to food allergens, IgG, IgA, IgM, IgE and immunoregulatory substances IL-5, TGF β1, IL-10, IL-4 IL-2, IL-13 were determined by enzyme immunoassay. All the patients received rotational diet therapy based on immunoassay data in addition to medical treatment of the basis disease...
2014: Experimental & Clinical Gastroenterology
Astrid Hogenkamp, Leon M J Knippels, Johan Garssen, Betty C A M van Esch
BACKGROUND: The maternal environment and early life exposure affect immune development in offspring. OBJECTIVE: We investigated whether development of food allergy in offspring is affected by supplementing pregnant or lactating sensitized or nonsensitized mice with a mixture of nondigestible oligosaccharides. METHODS: Dams were sensitized intragastrically with ovalbumin before mating, with use of cholera toxin (CT) as an adjuvant. Nonsensitized dams received CT only...
May 2015: Journal of Nutrition
Yutaka Shimizu, Hiroshi Oda, Kohsuke Seiki, Hiroki Saeki
A novel enzyme-linked immunosorbent assay (ELISA) system has been established for selective detection of chum salmon (Oncorhynchus keta) yolk protein (SYP). Rabbit and rat polyclonal Immunoglobulin G antibodies to β'-component (the major allergic protein in fish roe; anti-β) were applied for designing the ELISA system. The sandwich ELISA using rabbit anti-β for the capture antibody and horseradish peroxidase-labeled F(ab')2 fragment of rat anti-β for the detection antibody obtained high sensitivity and narrow specificity for SYP...
August 15, 2015: Food Chemistry
Chad J Roy, Robert N Brey, Nicholas J Mantis, Kelly Mapes, Iliodora V Pop, Laurentiu M Pop, Stephen Ruback, Stephanie Z Killeen, Lara Doyle-Meyers, Heather S Vinet-Oliphant, Peter J Didier, Ellen S Vitetta
Ricin toxin (RT) is the second most lethal toxin known; it has been designated by the CDC as a select agent. RT is made by the castor bean plant; an estimated 50,000 tons of RT are produced annually as a by-product of castor oil. RT has two subunits, a ribotoxic A chain (RTA) and galactose-binding B chain (RTB). RT binds to all mammalian cells and once internalized, a single RTA catalytically inactivates all of the ribosomes in a cell. Administered as an aerosol, RT causes rapid lung damage and fibrosis followed by death...
March 24, 2015: Proceedings of the National Academy of Sciences of the United States of America
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