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Food specific immunoglobulin g

Baifen Song, Hongyu Cui, Longzhu Ju, Li Song, Lijie Tang, Yijing Li
We previously developed a stable and marker-free Lactobacillus casei strain (PPαT Δupp) that contained a chromosomally integrated expression cassette (PPαT) that enabled the surface expression of the Clostridium perfringens alpha toxin. To measure immune responses against the alpha toxin, specific-pathogen-free BALB/c mice were inoculated with L. casei PPαT Δupp by oral gavage. Then, specific immunoglobulin A (IgA) and immunoglobulin G (IgG) antibodies and cytokines were measured by enzyme-linked immunosorbent assay (ELISA) and flow cytometry (FCM)...
March 7, 2018: Microbial Pathogenesis
Qian Sun, Yao Du, Elizabeth A H Hall, Dong Luo, Gleb B Sukhorukov, Alexander F Routh
Colloidosomes have attracted considerable attention in recent years because of their potential applications in a range of industries, such as food, bioreactors and medicine. However, traditional polymer shell colloidosomes leak low molecular weight encapsulated materials due to their intrinsic shell permeability. Here, we report aqueous core colloidosomes coated with a gold shell, which make the capsules impermeable. The shells can be ruptured using ultrasound. The gold coated colloidosomes are prepared by making an aqueous core capsule with a polymer shell and then adding HAuCl4 , surfactant and l-ascorbic acid to form a second shell...
February 21, 2018: Soft Matter
Marilyn B Nourse, Kate Engel, Samartha G Anekal, Jocelyn A Bailey, Pradeep Bhatta, Devayani P Bhave, Shekar Chandrasekaran, Yutao Chen, Steven Chow, Ushati Das, Erez Galil, Xinwei Gong, Steven F Gessert, Kevin D Ha, Ran Hu, Laura Hyland, Arvind Jammalamadaka, Karthik Jayasurya, Timothy M Kemp, Andrew N Kim, Lucie S Lee, Yang Lily Liu, Alphonso Nguyen, Jared O'Leary, Chinmay H Pangarkar, Paul J Patel, Ken Quon, Pradeep L Ramachandran, Amy R Rappaport, Joy Roy, Jerald F Sapida, Nikolay V Sergeev, Chandan Shee, Renuka Shenoy, Sharada Sivaraman, Bernardo Sosa-Padilla, Lorraine Tran, Amanda Trent, Thomas C Waggoner, Dariusz Wodziak, Amy Yuan, Peter Zhao, Daniel L Young, Channing R Robertson, Elizabeth A Holmes
The ability to perform laboratory testing near the patient and with smaller blood volumes would benefit patients and physicians alike. We describe our design of a miniaturized clinical laboratory system with three components: a hardware platform (ie, the miniLab) that performs preanalytical and analytical processing steps using miniaturized sample manipulation and detection modules, an assay-configurable cartridge that provides consumable materials and assay reagents, and a server that communicates bidirectionally with the miniLab to manage assay-specific protocols and analyze, store, and report results (i...
January 2018: Bioengineering & Translational Medicine
Xiaoxiao Su, Mengyao Wang, Yue Wu, Yong He, Zhifeng Fu
A novel dual-site recognition protocol was developed for chemiluminescent (CL) detection of Streptococcus mutans (S. mutans) based on a designed antibiotic-affinity strategy. Teicoplanin, a broad-spectrum antibiotic against Gram-positive bacteria, was adopted to functionalize magnetic particles and recognize S. mutans utilizing the strong affinity between this agent and D-Alanyl-D-Alanine peptide moieties in the bacterial cell wall. To achieve ideal specificity for S. mutans detection, rat immunoglobulin G2a (rat IgG2a) tagged with horseradish peroxidase (HRP) was used as the second recognition agent and signal tracer since Fab region of rat IgG2a could bind with streptococcal protein G highly expressed in the cell wall of S...
March 1, 2018: Talanta
Eishi Makita, Noriyuki Yanagida, Sakura Sato, Tomoyuki Asaumi, Motohiro Ebisawa
BACKGROUND: Anaphylaxis and immediate-type fish roe allergies have been reported worldwide, and, in Japan, fish roe is the sixth most common food allergen. No oral food challenges (OFCs) have used pollock roe (PR), which is reported to have high cross-reactivity with salmon roe (SR). Therefore, we administered an OFC using cooked PR to evaluate PR- and SR-specific immunoglobulin E (IgE) levels and allergic reactions in patients with PR sensitivity. METHODS: This retrospective study evaluating patient characteristics and responses to OFCs was conducted with 10-20 g of cooked PR, between April 2006 and November 2016...
December 11, 2017: Allergology International: Official Journal of the Japanese Society of Allergology
Camille Martin, Claire Kizlik-Masson, André Pèlegrin, Hervé Watier, Marie-Claude Viaud-Massuard, Nicolas Joubert
The annual "Antibody Industrial Symposium", co organized by LabEx MAbImprove, MabDesign and Polepharma, was held in Tours, France on June 27-28, 2017. The focus was on antibody-drug-conjugates (ADCs), new entities which realize the hope of Paul Ehrlich's magic bullet. ADCs result from the bioconjugation of a highly cytotoxic drug to a selective monoclonal antibody, which acts as a vector. Building on knowledge gained during the development of three approved ADCs, brentuximab vedotin (Adcetris®), ado trastuzumab emtansine (Kadcyla®) and inotuzumab ozogamicin (Besponsa®), and the many ADCs in development, this meeting addressed strategies and the latest innovations in the field from fundamental research to manufacturing...
December 14, 2017: MAbs
F Ahmadizar, S J H Vijverberg, H G M Arets, A de Boer, J E Lang, J Garssen, A Kraneveld, A H Maitland-van der Zee
This study systematically reviewed and quantified the relationship between exposure to antibiotics during the first 2 years of life and the risk of allergies/atopies including hay fever, eczema, food allergy, positive skin prick testing (SPT), or elevated allergen-specific serum/plasma immunoglobulin (Ig) E levels later in life. PubMed and Web of Science databases were searched for observational studies published from January 1966 through November 11, 2015. Overall pooled estimates of the odds ratios (ORs) were obtained using fixed or random-effects models...
November 6, 2017: Allergy
Przemyslaw Zdziarski, Andrzej Gamian, Jacek Majda, Agnieszka Korzeniowska-Kowal
BACKGROUND: Allergic, especially anaphylactic, reactions during immunoglobulin replacement therapy are rare, but their pathophysiology and classification remain ambiguous. Recent findings show positive results of skin tests with commercially available immunoglobulins, but target antigens and responsible compounds of the tested immunoglobulins have not been strictly identified. CASE DESCRIPTION AND FINDINGS: Four adult patients with recently diagnosed common variable immunodeficiency qualified for standard subcutaneous immunoglobulin replacement therapy regimen...
2017: Allergy, Asthma, and Clinical Immunology
H Guo, Y Zheng, X Cai, H Yang, Y Zhang, L Hao, Y Jin, G Yang
BACKGROUND: Low vitamin D status is linked to increased incidence of food allergy and intestinal inflammation. Whether vitamin D status is associated with immunological changes in children with gastrointestinal food allergy (GFA) remains unclear. METHODS: Forty-nine GFA children (aged 2-11 years old) were enrolled in this study. Serum 25-hydroxyvitamin D (25OHD) level, total immunoglobulin E (IgE), specific IgE against allergens, circulating regulatory T lymphocytes (Tregs), and blood eosinophil numbers were measured...
July 27, 2017: Allergologia et Immunopathologia
Noriyuki Yanagida, Sakura Sato, Tomoyuki Asaumi, Kiyotake Ogura, Motohiro Ebisawa
BACKGROUND: Severe anaphylactic symptoms can occur during oral food challenges (OFCs). Thus, high-risk patients (e.g., patients with a history of anaphylaxis or high antigen-specific immunoglobulin E [IgE] levels) must carefully undergo OFCs in hospitals. We attempted to identify the risk factors for severe symptoms during OFC testing among high-risk patients. METHODS: We retrospectively evaluated patients' characteristics and severe symptoms that were experienced during a double-blind placebo-controlled food challenge test performed before the patients underwent oral immunotherapy between June 2008 and June 2012...
2017: International Archives of Allergy and Immunology
Roberto Papini, Pierluigi di Ciccio, Marianna Marangi, Sergio Ghidini, Emanuela Zanardi, Alberto Vergara, Annunziata Giangaspero, Simona Nardoni, Guido Rocchigiani, Francesca Mancianti, Adriana Ianieri
To evaluate the occurrence of Toxoplasma gondii and to genetically characterize its isolates in carcasses of industrial fattening pigs, blood, diaphragm, and heart samples were collected from 375 carcasses of pigs slaughtered to be processed for Parma ham production. Pigs had been bred on approved farms (n = 75) located in the so-called Food Valley in Italy. Sera were examined for immunoglobulin G antibodies to T. gondii by modified agglutination test (MAT). Both heart and diaphragm samples from seropositive carcasses were processed for the presence of T...
March 2017: Journal of Food Protection
Masahiro Satake, Keiji Matsubayashi, Yuji Hoshi, Rikizo Taira, Yasumi Furui, Norihiro Kokudo, Nobuhisa Akamatsu, Tomoharu Yoshizumi, Nobuhiro Ohkohchi, Hiroaki Okamoto, Masato Miyoshi, Akinori Tamura, Kyoko Fuse, Kenji Tadokoro
BACKGROUND: The high prevalence of specific immunoglobulin G for hepatitis E virus (HEV) in Japanese people raises the possibility of a high incidence of HEV-viremic blood donors and therefore frequent transfusion-transmitted HEV (TT-HEV). STUDY DESIGN AND METHODS: TT-HEV cases established in Japan through hemovigilance and those published in the literature were collected. Infectivity of HEV-contaminated blood components and disease severity in relation to immunosuppression were investigated...
February 2017: Transfusion
Mitat Aricigil, Nuray Bayar Muluk, Engin Umut Sakarya, Emine Güven Sakalar, Mehmet Senturk, William R Reisacher, Cemal Cingi
OBJECTIVES: Allergen immunotherapy is the only cure for immunoglobulin E mediated type I respiratory allergies. Subcutaneous immunotherapy (SCIT) and sublingual immunotherapy (SLIT) are the most common treatments. In this article, we reviewed new routes of allergen immunotherapy. METHODS: Data on alternative routes to allow intralymphatic immunotherapy (ILIT), epicutaneous immunotherapy (EPIT), local nasal immunotherapy (LNIT), oral immunotherapy (OIT), and oral mucosal immunotherapy (OMIT) were gathered from the literature and were discussed...
November 1, 2016: American Journal of Rhinology & Allergy
Laura K Allred, Katherine Lesko, Diane McKiernan, Cynthia Kupper, Stefano Guandalini
Enzymatic digestion, or hydrolysis, has been proposed for treating gluten-containing foods and beverages to make them safe for persons with celiac disease (CD). There are no validated testing methods that allow the quantitation of all the hydrolyzed or fermented gluten peptides in foods and beverages that might be harmful to CD patients, making it difficult to assess the safety of hydrolyzed products. This study examines an ELISA-based method to determine whether serum antibody binding of residual peptides in a fermented barley-based product is greater among active-CD patients than a normal control group, using commercial beers as a test case...
March 1, 2017: Journal of AOAC International
Thomas B Martins, Michael E Bandhauer, Diane M Wilcock, Harry R Hill, Patricia R Slev
BACKGROUND: The clinical utility of serum IgG measurement in the diagnosis of allergy and food-induced hypersensitivity has been largely discredited. Recent studies, however, have shown that specific IgG can inhibit IgE mediated allergies, and may play a role in allergen specific desensitization. Accurate reference intervals for IgG specific allergens have not been widely established and are needed for better interpretation of serum antibody concentrations. In this study we established 64 IgG reference intervals for 48 common food allergens, 5 venoms, and 11 molds...
December 2016: Annals of Clinical and Laboratory Science
Laura Kwiatkowski, Jessica Mitchell, Jeffrey Langland
Context • Allergies are a common affliction, whether they are respiratory, food related, or dermatological. People often resort to continuous use of over-the-counter medications, such as antihistamines, to manage their symptoms. Controversy still remains over testing serum immunoglobulin (Ig) G to diagnose food allergies. Objective • This study intended to examine the benefits of treatment of a pediatric patient with natural supplements and an elimination diet for IgG food allergies. Design • The research team reported a case study...
October 2016: Alternative Therapies in Health and Medicine
Seyed Hossein Hoseinifar, Fazel Zoheiri, Carlo C Lazado
Immunostimulation through the use of sustainable and eco-friendly dietary additives is one of the current prophylactic strategies in fish husbandry. Plant-based immunostimulants are highly considered for this intent, both for their scientific and practical advantages. Persian hogweed (Heracleum persicum) is a flower-bearing herb that possesses interesting pharmacological importance due to its bioactive compounds. It is commonly used as spice, food additive, dietary supplement and traditional remedy. The present study evaluated the potential of H...
December 2016: Fish & Shellfish Immunology
Jacek Gocki, Zbigniew Bartuzi
This paper presents current views on the role of immunoglobulin G (IgG) antibodies in the reactions with food antigens in the digestive tract and their role in the diagnosis of food allergy based on the assays of specific IgG class antibodies, with a special focus on contemporary practice guidelines. In the light of current scientific knowledge, the IgG-specific antibody-mediated reactions are a body's natural and normal defensive reactions to infiltrating food antigens, which are considered as pathogens. On the other hand, specific IgG antibodies against food allergens play a crucial role in the induction and maintaining of immunological tolerance to food antigens...
August 2016: Postȩpy Dermatologii i Alergologii
Venkatachalam Sukumaran, Se Chang Park, Sib Sankar Giri
This study evaluated the effects of ginger (Zingiber officinale) as a feeding supplement on the growth, skin mucus immune parameters, and cytokine-related gene expression of Labeo rohita, and its susceptibility to Aeromonas hydrophila infection. Diets containing six different concentrations of dried ginger (0% [basal diet], 0.2% [G2], 0.4% [G4], 0.6% [G6], 0.8% [G8], and 1.0% [G10] were fed to fish (average weight: 12.3 g) for 60 days. Growth parameters were examined at 30 and 60 days post-feeding. Skin mucosal immune responses and gene expression were examined 60 days post-feeding...
October 2016: Fish & Shellfish Immunology
Xinjie Song, Shruti Shukla, Gibaek Lee, Sunhyun Park, Myunghee Kim
Cronobacter species (Cronobacter spp.) are hazardous foodborne pathogens associated with baby food, powdered infant formula (PIF). To develop a rapid and sensitive method for simultaneous detection of seven Cronobacter spp. in PIF, an indirect non-competitive enzyme-linked immunosorbent assay (INC-ELISA) was developed based on a novel immunoglobulin G (IgG), anti-Cronobacter IgG. The developed INC-ELISA was able to detect seven Cronobacter spp. at concentrations ranging from (5.6 ± 0.30) × 10(3) to (2.1 ± 0...
2016: Frontiers in Microbiology
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