keyword
https://read.qxmd.com/read/38481825/lesson-learned-from-mass-antibody-rapid-diagnostic-used-in-the-early-covid-19-pandemic-in-indonesia-contributors
#1
JOURNAL ARTICLE
Agnes Rengga Indrati, Luhung Budiailmiawan, Louisa Markus, Johanis Johanis, Verina Logito, Aryati
INTRODUCTION: Laboratory examination is extremely important in handling the COVID-19 pandemic. In the first era of the pandemic, the molecular and antigen tests were limited. Hence, at that time, it was necessary to carry out antibody Rapid Diagnostic Tests (RDT). However, many antibody RDTs were yet to obtain Food and Drug Authorization (FDA)'s approval. PURPOSE: Therefore, The Indonesian Association of Clinical Pathology and Medical Laboratory (PDS PatKLIn) decided to conduct a validity test of RDT antibodies to find out the quality of SARS-CoV-2 diagnosis performance based on these RDTs used...
2024: Medical Devices: Evidence and Research
https://read.qxmd.com/read/38469303/gene-edited-pigs-a-translational-model-for-human-food-allergy-against-alpha-gal-and-anaphylaxis
#2
JOURNAL ARTICLE
Ying Wang, Miriam Hils, Andrea Fischer, Florian Wölbing, Tilo Biedermann, Angelika Schnieke, Konrad Fischer
The prevalence of food allergy is rising and is estimated to approach 10%. Red meat allergy is the first known food allergy elicited by immunoglobulin E (IgE) antibodies recognizing a carbohydrate. Due to the loss of function of the alpha-1,3-galactosyltransferase ( GGTA1) gene in humans, the disaccharide galactose-α-1,3-galactose (α-Gal) cannot be synthesized and therefore became immunogenic. IgE sensitization is elicited through the skin by repetitive tick bites transmitting α-Gal. The underlying mechanisms regarding innate and adaptive immune cell activation, including the B-cell isotype switch to IgE, are poorly understood, requiring further research and physiologically relevant animal models...
2024: Frontiers in Immunology
https://read.qxmd.com/read/38351430/development-of-a-competitive-chemiluminescent-assay-for-quantitative-determination-of-tp53-fusion-protein-using-reagent-strips
#3
JOURNAL ARTICLE
Linyu Wang, Zhifang He, Jianye Li
Recent studies have shown that almost half of all cancers occur due to DNA damage. For the early diagnosis of cancer, a highly sensitized and swift identification for TP53 is needed since the corresponding TP53 protein is effectively recognized as "the guardian of the genome." To improve the detection sensitivity, numerous analytical methods were previously used for the determination of the TP53 protein, including denaturing high-performance liquid chromatography and enzyme-linked immunosorbent assay (ELISA)...
February 14, 2024: Applied Biochemistry and Biotechnology
https://read.qxmd.com/read/38346116/defining-the-cross-reactivity-between-peanut-allergens-ara-h-2-and-ara-h-6-using-monoclonal-antibodies
#4
JOURNAL ARTICLE
Orlee Marini-Rapoport, Monica L Fernández-Quintero, Tarun Keswani, Guangning Zong, Jane Shim, Lars C Pedersen, Geoffrey A Mueller, Sarita U Patil
In peanut allergy, Arachis hypogaea 2 (Ara h 2) and Arachis hypogaea 6 (Ara h 6) are two clinically relevant peanut allergens with known structural and sequence homology and demonstrated cross-reactivity. We have previously utilized X-ray crystallography and epitope binning to define the epitopes on Ara h 2. We aimed to quantitatively characterize the cross-reactivity between Ara h 2 and Ara h 6 on a molecular level using human monoclonal antibodies (mAbs) and structural characterization of allergenic epitopes...
February 12, 2024: Clinical and Experimental Immunology
https://read.qxmd.com/read/38318181/the-%C3%AE-gal-epitope-the-cause-of-a-global-allergic-disease
#5
REVIEW
Marija Perusko, Jeanette Grundström, Maria Eldh, Carl Hamsten, Danijela Apostolovic, Marianne van Hage
The galactose-α-1,3-galactose (α-Gal) epitope is the cause of a global allergic disease, the α-Gal syndrome (AGS). It is a severe form of allergy to food and products of mammalian origin where IgE against the mammalian carbohydrate, α-Gal, is the cause of the allergic reactions. Allergic reactions triggered by parenterally administered α-Gal sources appear immediately, but those triggered via the oral route appear with a latency of several hours. The α-Gal epitope is highly immunogenic to humans, apes and old-world monkeys, all of which produce anti-α-Gal antibodies of the IgM, IgA and IgG subclasses...
2024: Frontiers in Immunology
https://read.qxmd.com/read/38270478/coated-sodium-butyrate-and-vitamin-d-3-supplementation-improve-gut-health-through-influencing-intestinal-immunity-barrier-and-microflora-in-early-stage-broilers
#6
JOURNAL ARTICLE
Hang Gao, Xingkai Zhao, Yizhe Guo, Zhendong Li, Zhenlei Zhou
BACKGROUND: Intestinal development and function are critical to maintaining sustained broiler growth. The present study aimed to evaluate the effects of coated sodium butyrate (CSB) and vitamin D3 (VD3 ) on the intestinal immunity, barrier, oxidative stress and microflora in early-stage broilers. In total, 192 one-day-old broilers were assigned to a 2 × 2 factorial design including two dietary supplements at two different levels, in which the main effects were VD3 (3000 or 5000 IU kg-1 ) and CSB (0 or 1 g kg-1 )...
January 25, 2024: Journal of the Science of Food and Agriculture
https://read.qxmd.com/read/38163625/potential-of-lactobacillus-plantarum-a56-in-relieving-food-allergy-through-immunoregulation-antioxidation-and-reshaping-intestinal-microbiota
#7
JOURNAL ARTICLE
Cuicui Duan, Lin Ma, Mengchun Qin, Lingfang Zhang, Shunan Hu, Lifan Liu, Yixue Sun, Fumin Ma, Dan Li
Food allergy is an abnormal immune reaction triggered by food protein antigens. Relevant studies have suggested that probiotic supplementation was with the potential to alleviate food allergy. This study aimed to explore the effects of Lactobacillus plantarum A56 on the alleviation of ovalbumin (OVA)-induced food allergy via immunomodulatory function, antioxidation and modification of intestinal microbiota. Balb/c mice were sensitized with OVA (20μg/mouse) by intraperitoneal injection for three weeks and accompanied by oral administration of L...
December 30, 2023: Journal of Nutritional Biochemistry
https://read.qxmd.com/read/38027884/seroprevalence-of-hepatitis-e-virus-hev-among-male-craft-and-manual-workers-in-qatar-2020-2021
#8
JOURNAL ARTICLE
Nadin Younes, Hadi M Yassine, Parveen Banu Nizamuddin, Katerina Kourentzi, Patrick Tang, Houssein H Ayoub, Makiyeh Khalili, Peter V Coyle, Dmitri Litvinov, Richard C Willson, Laith J Abu-Raddad, Gheyath K Nasrallah
BACKGROUND: The rapid growth of Qatar in the last two decades has attracted a large influx of immigrant craft and manual workers (CMWs) seeking employment in jobs associated with food handling, domestic service, and construction. Nearly 60 % of Qatar's population are expatriates CMWs, including many from hyperendemic countries for HEV. Thus, estimating the seroprevalence of HEV in Qatar and understanding its epidemiology is essential for public health efforts to control HEV transmission in Qatar...
November 2023: Heliyon
https://read.qxmd.com/read/37981502/local-nasal-immunotherapy-with-birch-pollen-galactomannan-conjugate-containing-ointment-in-mice-and-humans
#9
JOURNAL ARTICLE
Keiko Komatsuzaki, Hiroki Kageshima, Yuki Sekino, Yasuhiro Suzuki, Tsukasa Ugajin, Meiyo Tamaoka, Ryoichi Hanazawa, Akihiro Hirakawa, Yasunari Miyazaki
BACKGROUND: Allergen immunotherapy (AIT) is the only disease-modifying treatment for immunoglobulin (Ig) E-mediated allergy. Owing to the high prevalence and early onset of hay fever and pollen-food allergy syndrome (PFAS), a safer and simpler treatment method than conventional AIT is needed. To develop a local nasal immunotherapy using an ointment containing hypoallergenic pollen and assess its efficacy in mice and healthy humans. METHODS: Hypoallergenicity was achieved by combining pollen and galactomannan through the Maillard reaction to create birch pollen-galactomannan conjugate (BP-GMC)...
November 17, 2023: Allergology International: Official Journal of the Japanese Society of Allergology
https://read.qxmd.com/read/37943604/serological-and-molecular-detection-of-toxoplasma-gondii-in-domestic-pigs-intended-for-human-consumption-and-potential-occupational-hazard-to-pig-farmers-in-india
#10
JOURNAL ARTICLE
Megha Roy, Veena Mishra, Pallabi Mitra, Yeshwant Umbardand, Vaishnavi Sapate, Waqar Khan, Abhijit S Deshmukh
Toxoplasma gondii , an important food-borne zoonotic parasite, poses a worldwide public health hazard. Domestic pigs are considered one of the main intermediate hosts in the zoonotic transmission of T. gondii . To date, seroepidemiological information on T. gondii in domestic pigs in India is very scarce, and there are no reports of occupational hazards to pig farmers in this country. Here, we aimed at estimating the occurrence of T. gondii (antibodies and parasite DNA) in slaughtered pigs and pig farmers in Central India...
November 9, 2023: Foodborne Pathogens and Disease
https://read.qxmd.com/read/37943403/anti-cryptosporidium-oocysts-polyclonal-antibodies-for-cryptosporidiosis-diagnosis-and-protection
#11
JOURNAL ARTICLE
Alyaa Farid, Silvia Aiad, Gehan Safwat
Cryptosporidiosis is an intestinal infection that is triggered by the protozoan parasite Cryptosporidium spp. Cryptosporidium oocysts can spread from one host to another either through direct contact with infected hosts' faeces or through indirect means (consumption of contaminated water or food). Significant numbers of oocysts are produced as a result of the rapid growth of the parasite within the infected hosts. For proper care of cryptosporidiosis, a laboratory diagnosis is necessary...
November 9, 2023: AMB Express
https://read.qxmd.com/read/37941425/efficient-and-rapid-linker-optimization-with-heterodimeric-coiled-coils-improves-the-response-of-fluorescent-biosensors-comprising-antibodies-and-protein-m
#12
JOURNAL ARTICLE
Kana Sasamoto, Takanobu Yasuda, Bo Zhu, Hiroshi Ueda, Tetsuya Kitaguchi
We developed a coiled Q-probe (CQ-probe), a fluorescent probe containing a coiled-coil peptide pair E4/K4, to convert antibodies into biosensors for homogeneous immunoassays. This probe consists of an antibody-binding protein, protein M (PM) with the E4 peptide and the K4 peptide with a fluorescent dye. Compared to PM Q-probes, which are generated by modifying the C-terminus of PM with a fluorescent dye, CQ-probe variants with various linkers are easy to prepare and therefore enable the establishment of biosensors with a significant fluorescence response by localizing the fluorescent dye at the optimal position for quenching and antigen-dependent release...
November 9, 2023: Analyst
https://read.qxmd.com/read/37918164/an-automatic-and-smart-platform-for-rapid-detection-of-cadmium-and-lead-simultaneously-in-rice-using-triple-amplified-chemiluminescence-immunoassay
#13
JOURNAL ARTICLE
Wei Tian, Songxue Wang, Xiaomeng Li, Minghui Zhou, Yanxiang Wu, Jieqiong Zhang, Xi Chen
Rapid detection of trace ions is urgently needed for large-scale screening to ensure food safety. This study developed an innovative and automatic strategy, based on a smart-designed platform for rapid detection of cadmium and lead in rice. As bridge antibody, the antigen was conjugated with goat anti-mouse immunoglobulin G labeled alkaline phosphatase. Meanwhile, a biotin-streptavidin system was introduced to micromagnetic particles, thus providing a triple-amplified chemiluminescence immunoassay with high sensitivity, accuracy and specificity...
October 30, 2023: Food Chemistry
https://read.qxmd.com/read/37868190/development-and-evaluation-of-an-immunochromatography-based-point-of-care-test-kit-for-a-rapid-diagnosis-of-human-cysticercosis
#14
JOURNAL ARTICLE
Lakkhana Sadaow, Patcharaporn Boonroumkaew, Rutchanee Rodpai, Penchom Janwan, Oranuch Sanpool, Tongjit Thanchomnang, Yasuyuki Morishima, Marcello Otake Sato, Yasuhito Sako, Kaoru Kobayashi, Misako Iwai, Wanchai Maleewong, Hiroshi Yamasaki, Pewpan M Intapan
Human cysticercosis is a life-threatening zoonotic disease caused by infection with larvae (cysticerci) of the pork tapeworm, Taenia solium . This can affect the nervous system causing chronic headache and intracranial hypertension, potentially leading to epileptic seizures and paralysis. The disease is found in developing countries, especially in Southeast and South Asia, Sub-Saharan Africa, and Central and South America where porcine cysticercosis is endemic and people have a habit of eating undercooked pork...
December 2023: Food and waterborne parasitology
https://read.qxmd.com/read/37833865/assessment-of-the-immunoprotective-efficacy-of-recombinant-14-3-3-protein-and-dense-granule-protein-10-gra10-as-candidate-antigens-for-rabbit-vaccines-against-eimeria-intestinalis
#15
JOURNAL ARTICLE
Changming Xiong, Wei He, Jie Xiao, Ge Hao, Jiayan Pu, Hao Chen, Liwen Xu, Yuhua Zhu, Guangyou Yang
Eimeria intestinalis infects rabbits, causing severe intestinal coccidiosis. Prolonged anticoccidial drug use might lead to coccidia resistance and drug residues in food. Thus, vaccines are required to control rabbit coccidiosis. In this study, recombinant E. intestinalis 14-3-3 and GRA10 proteins (r Ei -14-3-3 and r Ei -GRA10) were obtained via prokaryotic expression and used as recombinant subunit vaccines. Fifty 30-day-old rabbits were randomly grouped as follows: PBS-uninfected group, PBS-infected group, Trx-His-S control group, and r Ei -14-3-3 and r Ei -GRA10 immunized groups...
September 22, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/37815010/influence-of-antigen-density-and-tlr-ligands-on-preclinical-efficacy-of-a-vlp-based-vaccine-against-peanut-allergy
#16
JOURNAL ARTICLE
Pascal S Krenger, Romano Josi, Jan Sobczak, Thalia L C Velazquez, Ina Balke, Murray A Skinner, Matthias F Kramer, Callum J W Scott, Simon Hewings, Matthew D Heath, Andris Zeltins, Martin F Bachmann
BACKGROUND: Virus-like particle (VLP) Peanut is a novel immunotherapeutic vaccine candidate for the treatment of peanut allergy. The active pharmaceutical ingredient represents cucumber mosaic VLPs (CuMVTT -VLPs) that are genetically fused with one of the major peanut allergens, Ara h 2 (CuMVTT -Ara h 2). We previously demonstrated the immunogenicity and the protective capacity of VLP Peanut-based immunization in a murine model for peanut allergy. Moreover, a Phase I clinical trial has been initiated using VLP Peanut material manufactured following a GMP-compliant manufacturing process...
October 10, 2023: Allergy
https://read.qxmd.com/read/37726342/serum-food-specific-igg-antibodies-are-associated-with-small-bowel-inflammation-in-patients-with-crohn-s-disease
#17
JOURNAL ARTICLE
Jintong Chen, Huaning Chen, Yanni Huang, Hongchai Xie, Silin Li, Chengdang Wang
BACKGROUND/AIMS: Food antigens are thought to play a vital role in the initiation and perpetuation of Crohn's disease (CD). The main purpose of this study was to evaluate the potential association of serum food specific IgG antibodies and small bowel (SB) inflammation in CD patients. METHODS: We conducted a prospective observational study with 96 CD patients. Demographic, disease-related data and inflammatory parameters were collected. Serum food IgG antibodies were measured using enzyme-linked immunosorbent assay (ELISA)...
September 19, 2023: European Journal of Clinical Nutrition
https://read.qxmd.com/read/37719690/human-gnathostomiasis-a-review-on-the-biology-of-the-parasite-with-special-reference-on-the-current-therapeutic-management
#18
REVIEW
Kathyleen Nogrado, Poom Adisakwattana, Onrapak Reamtong
Gnathostoma is a parasitic nematode that can infect a wide range of animal species, but human populations have become accidental hosts because of their habit of eating raw or undercooked meat from a wide variety of intermediate hosts. While gnathostomiasis is considered an endemic disease, cases of human gnathostomiasis have been increasing over time, most notably in nonendemic areas. There are several complexities to this parasitic disease, and this review provides an update on human gnathostomiasis, including the life cycle, diagnosis, treatment, and treatment strategies used to combat drug resistance...
December 2023: Food and waterborne parasitology
https://read.qxmd.com/read/37628047/insights-into-the-mechanism-underlying-the-influence-of-glycation-with-different-saccharides-and-temperatures-on-the-igg-ige-binding-ability-immunodetection-in-vitro-digestibility-of-shrimp-litopenaeus-vannamei-tropomyosin
#19
JOURNAL ARTICLE
Jinlong Zhao, Jin Wang, Lili Xu, Hao Wang, Ziye Zhang, Hong Lin, Zhenxing Li
Tropomyosin (TM) is a heat-stable protein that plays a crucial role as a major pan-allergen in crustacean shellfish. Despite the high thermal stability of the TM structure, its IgG/IgE binding ability, immunodetection, and in vitro digestibility can be negatively influenced by glycation during food processing, and the underlying mechanism remains unclear. In this study, TM was subjected to glycosylation using various sugars and temperatures. The resulting effects on IgG/IgE-binding capacity, immunodetection, and in vitro digestibility were analyzed, meanwhile, the structural alterations and modifications using spectroscopic and LC-MS/MS analysis were determined...
August 14, 2023: Foods (Basel, Switzerland)
https://read.qxmd.com/read/37497566/immunoglobulin-g4-in-eosinophilic-esophagitis-immune-complex-formation-and-correlation-with-disease-activity
#20
JOURNAL ARTICLE
Jonathan G Medernach, Rung-Chi Li, Xiao-Yu Zhao, Bocheng Yin, Emily A Noonan, Elaine F Etter, Shyam S Raghavan, Larry C Borish, Jeffrey M Wilson, Barrett H Barnes, Thomas A E Platts-Mills, Sarah E Ewald, Bryan G Sauer, Emily C McGowan
BACKGROUND: Recent studies have shown deposition of immunoglobulin G4 (IgG4) and food proteins in the esophageal mucosa of eosinophilic esophagitis (EoE) patients. Our aims were to assess whether co-localization of IgG4 and major cow's milk proteins (CMPs) was associated with EoE disease activity and to investigate the proteins enriched in proximity to IgG4 deposits. METHODS: This study included adult subjects with EoE (n = 13) and non-EoE controls (n = 5)...
July 27, 2023: Allergy
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