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Peripheral blood stem cells

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https://www.readbyqxmd.com/read/29907809/killer-cell-immunoglobulin-like-receptor-ligand-mismatching-and-outcome-after-haploidentical-transplantation-with-post-transplant-cyclophosphamide
#1
Avichai Shimoni, Myriam Labopin, Francesca Lorentino, Maria Teresa Van Lint, Yener Koc, Zafer Gülbas, Johanna Tischer, Benedetto Bruno, Didier Blaise, Pietro Pioltelli, Boris Afanasyev, Fabio Ciceri, Mohamad Mohty, Arnon Nagler
Haploidentical stem cell transplantation with T cell-replete grafts and post-transplant cyclophosphamide (PTCy) is increasingly used with encouraging outcome. Natural killer (NK) cell alloreactivity, predicted by missing killer cell immunoglobulin-like receptor (KIR) ligands in the recipient that are present in their donor improves outcome of T cell-depleted haploidentical transplants. We explored the role of KIR ligand mismatching in 444 acute leukemia patients after T cell-replete transplants with PTCy. Thirty-seven percent of all patients had KIR ligand mismatching...
June 15, 2018: Leukemia: Official Journal of the Leukemia Society of America, Leukemia Research Fund, U.K
https://www.readbyqxmd.com/read/29907164/optimizing-the-method-for-generation-of-integration-free-induced-pluripotent-stem-cells-from-human-peripheral-blood
#2
Haihui Gu, Xia Huang, Jing Xu, Lili Song, Shuping Liu, Xiao-Bing Zhang, Weiping Yuan, Yanxin Li
BACKGROUND: Generation of induced pluripotent stem cells (iPSCs) from human peripheral blood provides a convenient and low-invasive way to obtain patient-specific iPSCs. The episomal vector is one of the best approaches for reprogramming somatic cells to pluripotent status because of its simplicity and affordability. However, the efficiency of episomal vector reprogramming of adult peripheral blood cells is relatively low compared with cord blood and bone marrow cells. METHODS: In the present study, integration-free human iPSCs derived from peripheral blood were established via episomal technology...
June 15, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29906526/promidis%C3%AE-a-tcr%C3%AE-signature-associated-with-immunodeficiencies-caused-by-v-d-j-recombination-defects
#3
Aurélie Berland, Jérémie Rosain, Sophie Kaltenbach, Vincent Allain, Nizar Mahlaoui, Isabelle Melki, Alice Fievet, Catherine Dubois d'Enghien, Marie Ouachée-Chardin, Laurence Perrin, Nathalie Auger, Funda Erol Cipe, Andrea Finocchi, Figen Dogu, Felipe Suarez, Despina Moshous, Thierry Leblanc, Alexandre Belot, Claire Fieschi, David Boutboul, Marion Malphettes, Lionel Galicier, Eric Oksenhendler, Stéphane Blanche, Alain Fischer, Patrick Revy, Dominique Stoppa-Lyonnet, Capucine Picard, Jean-Pierre de Villartay
- BACKGROUND: V(D)J recombination ensures the diversity of the adaptive immune system. While its complete defect causes Severe Combined Immunodeficiency (T-B-SCID), its suboptimal activity, is associated with a broad spectrum of immune manifestations such as late onset combined immunodeficiency and autoimmunity. The earliest molecular diagnosis of these patients is required to adopt the best therapy strategy, in particular when it involves myelo-ablative conditioning regimen for hematopoietic stem cell transplantation (HSCT)...
June 12, 2018: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29905053/-effect-of-human-adipose-derived-stem-cells-on-pressure-ulcer-healing-in-mouse
#4
Chengliang Deng, Zhiyuan Liu, Yuanzhen Yao, Ruichi Liu, Zairong Wei, Dali Wang
Objective: To investigate the effect of human adipose-derived stem cells (hADSCs) on pressure ulcers in mouse. Methods: The subcutaneous adipose tissue from voluntary donation was harvested. Then the hADSCs were isolated and cultured by mechanical isolation combined with typeⅠcollagenase digestion. The 3rd generation cells were identified by osteogenic, adipogenic, chondrogenic differentiations and flow cytometry. The platelet rich plasma (PRP) from peripheral blood donated by healthy volunteers was prepared by centrifugation...
June 1, 2018: Chinese Journal of Reparative and Reconstructive Surgery
https://www.readbyqxmd.com/read/29900128/residual-peripheral-blood-cd26-leukemic-stem-cells-in-chronic-myeloid-leukemia-patients-during-tki-therapy-and-during-treatment-free-remission
#5
Monica Bocchia, Anna Sicuranza, Elisabetta Abruzzese, Alessandra Iurlo, Santina Sirianni, Antonella Gozzini, Sara Galimberti, Lara Aprile, Bruno Martino, Patrizia Pregno, Federica Sorà, Giulia Alunni, Carmen Fava, Fausto Castagnetti, Luca Puccetti, Massimo Breccia, Daniele Cattaneo, Marzia Defina, Olga Mulas, Claudia Baratè, Giovanni Caocci, Simona Sica, Alessandro Gozzetti, Luigiana Luciano, Monica Crugnola, Mario Annunziata, Mario Tiribelli, Paola Pacelli, Ilaria Ferrigno, Emilio Usala, Nicola Sgherza, Gianantonio Rosti, Alberto Bosi, Donatella Raspadori
Chronic myeloid leukemia (CML) patients in sustained "deep molecular response" may stop TKI treatment without disease recurrence; however, half of them lose molecular response shortly after TKI withdrawing. Well-defined eligibility criteria to predict a safe discontinuation up-front are still missing. Relapse is probably due to residual quiescent TKI-resistant leukemic stem cells (LSCs) supposedly transcriptionally low/silent and not easily detectable by BCR-ABL1 qRT-PCR. Bone marrow Ph+ CML CD34+ /CD38- LSCs were found to specifically co-express CD26 (dipeptidylpeptidase-IV)...
2018: Frontiers in Oncology
https://www.readbyqxmd.com/read/29900044/cd133-directed-car-t-cells-for-advanced-metastasis-malignancies-a-phase-i-trial
#6
Yao Wang, Meixia Chen, Zhiqiang Wu, Chuan Tong, Hanren Dai, Yelei Guo, Yang Liu, Jianhua Huang, Haiyan Lv, Can Luo, Kai-Chao Feng, Qing-Ming Yang, Xiao-Lei Li, Weidong Han
Expressed by cancer stem cells of various epithelial cell origins, CD133 is an attractive therapeutic target for cancers. Autologous chimeric antigen receptor-modified T-cell directed CD133 (CART-133) was first tested in this trial. The anti-tumor specificity and the postulated toxicities of CART-133 were first assessed. Then, we conducted a phase I clinical study in which patients with advanced and CD133-positive tumors received CART-133 cell-infusion. We enrolled 23 patients (14 with hepatocellular carcinoma [HCC], 7 with pancreatic carcinomas, and 2 with colorectal carcinomas)...
2018: Oncoimmunology
https://www.readbyqxmd.com/read/29899859/prostate-stem-cell-antigen-mrna-in-blood-is-a-predictor-of-survival-after-radical-prostatectomy-in-patients-with-high-risk-prostate-cancer
#7
Yoon Seok Suh, Jae Young Joung, Sung Han Kim, Jeong Eun Kim, Moon Kyung Choi, Weon Seo Park, Sang-Jin Lee, Ho Kyung Seo, Jinsoo Chung, Kang Hyun Lee
Objectives: To investigate whether the preoperative detection of prostate stem cell antigen (PSCA) mRNA in blood has predictive value for biochemical recurrence, overall survival, and cancer-specific survival after radical prostatectomy in patients with high-risk prostate cancer. Results: Median age was 67 years (interquartile range: 63-71), and median follow-up was 41 months (interquartile range: 25-65). PSCA mRNA was detected in 151 patients (51.1%). Biochemical recurrence was developed in 101 patients (34...
May 29, 2018: Oncotarget
https://www.readbyqxmd.com/read/29896671/betaherpesvirus-complications-and-management-during-hematopoietic-stem-cell-transplantation
#8
Tetsushi Yoshikawa
Two of the four betaherpesviruses, Cytomegalovirus (CMV) and human herpesvirus 6B (HHV-6B), play an important role in opportunistic infections in hematopoietic stem cell transplant (HSCT) recipients. These viruses are ubiquitous in humans and can latently infect mononuclear lymphocytes, complicating the diagnosis of the diseases they cause. Although the detection of viral DNA in a patient's peripheral blood by real-time PCR is widely used for monitoring viral infection, it is insufficient for the diagnosis of virus-associated disease...
2018: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29895931/a-phase-iv-randomized-multicenter-open-label-trial-comparing-efficacy-and-systemic-exposure-for-a-standard-weight-based-dose-versus-a-fixed-dose-of-plerixafor-in-combination-with-g-csf-in-patients-with-non-hodgkin-s-lymphoma-weighing-%C3%A2-70-kg
#9
John Kuruvilla, Cheng-Hwai Tzeng, Seok-Goo Cho, Seok Jin Kim, Jih-Luh Tang, Yaming Su, Jingyang Wu, Rita Vargo, Peter Cheverton
A randomized, multicenter, open-label study explored the effect of a fixed-dose (FD) of plerixafor versus the approved weight-based (WB) dose for the mobilization of hematopoietic stem cells (HSCs) in patients with non-Hodgkin's lymphoma and a body weight of ≤70 kg. After mobilization with granulocyte colony-stimulating factor (G-CSF) 10 μg/kg/day for 4 days, patients were randomized 1:1 to either plerixafor FD 20 mg (n = 30) or WB 0.24 mg/kg (n = 31) on the evening of Day 4. Co-primary endpoints were the proportion of patients achieving ≥5 × 106 CD34+ cells/kg in ≤4 days of apheresis, and total systemic exposure to plerixafor (area under the concentration-time curve from 0 to 10 h [AUC0-10 ])...
June 12, 2018: Bone Marrow Transplantation
https://www.readbyqxmd.com/read/29893072/-process-in-menstrual-blood-derived-mesenchymal-stem-cells-for-treatment-of-central-nervous-system-diseases
#10
Mengmeng Liu, Xinran Cheng, Kaikai Li, Mingrui Xu, Yongji Wu, Mengli Wang, Qianru Zhang, Wenyong Yan, Chang Luo, Shanting Zhao
Stem cell research has become a frontier in the field of life sciences, and provides an ideal model for exploring developmental biology problems such as embryogenesis, histiocytosis, and gene expression regulation, as well as opens up new doors for clinical tissue defective and inheritance diseases. Among them, menstrual blood-derived stem cells (MenSCs) are characterized by wide source, multi-directional differentiation potential, low immune rejection characteristics. Thus, MenSCs can achieve individual treatment and have the most advantage of the clinical application...
May 25, 2018: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://www.readbyqxmd.com/read/29893031/human-primary-cd34-cells-transplantation-for-critical-limb-ischemia
#11
Weishuai Lian, Xiaoxiao Hu, Long Pan, Shilong Han, Chuanwu Cao, Zhongzhi Jia, Maoquan Li
BACKGROUND: The goal of this study was to characterize the properties of human CD34+ cells in culture and investigate the feasibility and efficacy of CD34+ transplantation in a mouse model of limb ischemia and in patients with no-option critical limb ischemia. METHODS: Human CD34+ cells isolated from peripheral blood and grown in culture for up to four passages stained positively for the surface markers CD34 and CD133 and showed high viability after cryopreservation and recovery...
June 11, 2018: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/29892010/lipoxin-a-4-attenuates-the-inflammatory-response-in-stem-cells-of-the-apical-papilla-via-alx-fpr2
#12
A Gaudin, M Tolar, O A Peters
Similar to the onset phase of inflammation, its resolution is a process that unfolds in a manner that is coordinated and regulated by a panel of mediators. Lipoxin A4 (LXA4 ) has been implicated as an anti-inflammatory, pro-resolving mediator. We hypothesized that LXA4 attenuates or prevents an inflammatory response via the immunosuppressive activity of Stem Cells of the Apical Papilla (SCAP). Here, we report for the first time in vitro that in a SCAP population, lipoxin receptor ALX/FPR2 was constitutively expressed and upregulated after stimulation with lipopolysaccharide and/or TNF-α...
June 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29891441/adverse-events-associated-with-infusion-of-hematopoietic-stem-cell-products-a-prospective-and-multicenter-surveillance-study
#13
REVIEW
Kazuhiko Ikeda, Hitoshi Ohto, Yoshiki Okuyama, Minami Yamada-Fujiwara, Heiwa Kanamori, Shin-Ichiro Fujiwara, Kazuo Muroi, Takehiko Mori, Kinuyo Kasama, Tohru Iseki, Tokiko Nagamura-Inoue, Nobuharu Fujii, Takashi Ashida, Kazuaki Kameda, Junya Kanda, Asao Hirose, Tsutomu Takahashi, Kazuhiro Nagai, Keiji Minakawa, Ryuji Tanosaki
Adverse events (AEs) associated with blood transfusions, including component-specific red cell, platelet, and plasma products, have been extensively surveyed. In contrast, surveillance of AEs associated with hematopoietic stem cell (HSC) products in HSC transplantation (HSCT) has been less rigorous, even though HSC products include a diversity of immature and mature hematopoietic cells, substantial plasma, and dimethyl sulfoxide (DMSO) in the case of cryopreserved HSC products. HSC infusion-related AEs have been attributed to DMSO toxicity, but AEs associated with the infusion of noncryopreserved HSC products are not uncommon...
June 1, 2018: Transfusion Medicine Reviews
https://www.readbyqxmd.com/read/29886240/s1p-promotes-migration-differentiation-and-immune-regulatory-activity-in-amniotic-fluid-derived-stem-cells
#14
Rita Romani, Giorgia Manni, Chiara Donati, Irene Pirisinu, Caterina Bernacchioni, Marco Gargaro, Matteo Pirro, Mario Calvitti, Giorgia Manni, Francesco Bagaglia, Amirhossein Sahebkar, Graziano Clerici, Davide Matino, Giovanni Pomili, Gian Carlo Di Renzo, Vincenzo Nicola Talesa, Paolo Puccetti, Francesca Fallarino
Stem cells have high potential for cell therapy in regenerative medicine. We previously isolated stem cell types from human amniotic fluid, derived from prenatal amniocentesis. One type, characterized by a fast doubling time, was designated as fast human amniotic stem cells (fHASCs). These cells exhibited high differentiation potential and immunoregulatory properties. Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid metabolite that influences stem-cell pluripotency, differentiation, mobility, and regulates immune functions...
June 7, 2018: European Journal of Pharmacology
https://www.readbyqxmd.com/read/29885947/a-transcriptomic-signature-predicting-septic-outcome-in-patients-undergoing-autologous-stem-cell-transplantation
#15
Labiad Yasmine, Venton Geoffroy, Farnault Laure, Baier Céline, Colle Julien, Mercier Cedric, Ivanov Vadim, Nicolino Corinne, Loriod Béatrice, Fernandez-Nunez Nicolas, Torres Magali, Mattei Jean-Camille, Rihet Pascal, Nguyen Catherine, Costello Régis
Autologous hematopoietic stem cell transplantation (auto-HSCT) is a standard treatment in multiple myeloma and relapsed or refractory lymphomas. After auto-HSCT, hematologic reconstitution and infectious complications are the main two critical issues. Though many patients develop infectious complications after therapeutic intensification, it remains impossible to predict infection for each individual. The goal of this work was to determine and identify a predictive transcriptomic signature of systemic inflammatory response syndrome (SIRS) and/or sepsis in patients receiving auto-HSCT...
June 7, 2018: Experimental Hematology
https://www.readbyqxmd.com/read/29884617/brain-targeted-stem-cell-gene-therapy-corrects-mucopolysaccharidosis-type-ii-via-multiple-mechanisms
#16
Hélène Fe Gleitz, Ai Yin Liao, James R Cook, Samuel F Rowlston, Gabriella Ma Forte, Zelpha D'Souza, Claire O'Leary, Rebecca J Holley, Brian W Bigger
The pediatric lysosomal storage disorder mucopolysaccharidosis type II is caused by mutations in IDS, resulting in accumulation of heparan and dermatan sulfate, causing severe neurodegeneration, skeletal disease, and cardiorespiratory disease. Most patients manifest with cognitive symptoms, which cannot be treated with enzyme replacement therapy, as native IDS does not cross the blood-brain barrier. We tested a brain-targeted hematopoietic stem cell gene therapy approach using lentiviral IDS fused to ApoEII (IDS...
June 8, 2018: EMBO Molecular Medicine
https://www.readbyqxmd.com/read/29884406/integrated-genomic-characterization-of-oral-carcinomas-in-post-hematopoietic-stem-cell-transplantation-survivors
#17
Glenn J Hanna, Eric R Kofman, Muhammad Ali Shazib, Sook-Bin Woo, Brendan Reardon, Nathaniel S Treister, Robert I Haddad, Corey S Cutler, Joseph H Antin, Eliezer M Van Allen, Ravindra Uppaluri, Robert J Soiffer
OBJECTIVES: Secondary oral squamous cell carcinoma (OSCC) is a late complication in allogeneic hematopoietic stem cell transplantation (HSCT) patients, but little is known about long-term outcomes and prognostication. Additionally, molecular alterations and immunologic insights unique to this disease remain largely unexplored. METHODS: We present a cohort of 31 patients with post-HSCT OSCC and reported on clinicopathologic predictors of survival. Whole-exome sequencing was performed on 6 (19%) matched pairs of peripheral blood (post-conditioning, pre-HSCT) and tumor samples...
June 2018: Oral Oncology
https://www.readbyqxmd.com/read/29884336/characterization-of-the-cellular-responses-of-dental-mesenchymal-stem-cells-to-the-immune-system
#18
Dean Whiting, Whasun Oh Chung, James D Johnson, Avina Paranjpe
INTRODUCTION: Dental stem cells have gained importance recently and are being used for various purposes in regenerative medicine and dentistry. Although much research has been done to show the various properties of these dental stem cells, the immunomodulatory properties of some of these stem cells are still unknown. This is important considering these cells are being used routinely. Therefore, the aim of this study was to investigate the interactions between the activated immune cells and 3 types of dental-derived mesenchymal stem cells: dental pulp stem cells, stem cells from human exfoliated deciduous teeth, and stem cells of the apical papilla (SCAP)...
June 5, 2018: Journal of Endodontics
https://www.readbyqxmd.com/read/29883886/ipsc-derived-neurons-of-crebbp-and-ep300-mutated-rubinstein-taybi-syndrome-patients-show-morphological-alterations-and-hypoexcitability
#19
Valentina Alari, Silvia Russo, Benedetta Terragni, Paola Francesca Ajmone, Alessandra Sironi, Ilaria Catusi, Luciano Calzari, Daniela Concolino, Rosa Marotta, Donatella Milani, Daniela Giardino, Massimo Mantegazza, Cristina Gervasini, Palma Finelli, Lidia Larizza
Rubinstein-Taybi syndrome (RSTS) is a rare neurodevelopmental disorder characterized by distinctive facial features, growth retardation, broad thumbs and toes and mild to severe intellectual disability, caused by heterozygous mutations in either CREBBP or EP300 genes, encoding the homologous CBP and p300 lysine-acetyltransferases and transcriptional coactivators. No RSTS in vitro induced Pluripotent Stem Cell (iPSC)-neuronal model is available yet to achieve mechanistic insights on cognitive impairment of RSTS patients...
May 30, 2018: Stem Cell Research
https://www.readbyqxmd.com/read/29882300/critical-assessment-of-the-efficiency-of-cd34-and-cd133-antibodies-for-enrichment-of-rabbit-hematopoietic-stem-cells
#20
Jaromír Vašíček, Medhat Shehata, Susanne Schnabl, Martin Hilgarth, Rainer Hubmann, Ulrich Jäger, Miroslav Bauer, Peter Chrenek
Rabbits have many hereditary diseases common to humans and are therefore a valuable model for regenerative disease and hematopoietic stem cell (HSC) therapies. Currently, there is no substantial data on the isolation and/or enrichment of rabbit HSCs. This study was initiated to evaluate the efficiency of the commercially available anti-CD34 and anti-CD133 antibodies for the detection and potential enrichment of rabbit HSCs from peripheral blood. PBMCs from rabbit and human blood were labelled with different clones of anti-human CD34 monoclonal antibodies (AC136, 581 and 8G12) and rabbit polyclonal CD34 antibody (pCD34) and anti-human CD133 monoclonal antibodies (AC133 and 293C3)...
June 8, 2018: Biotechnology Progress
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