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https://www.readbyqxmd.com/read/28063951/transcriptomic-analysis-of-spleen-infected-with-infectious-salmon-anemia-virus-reveals-distinct-pattern-of-viral-replication-on-resistant-and-susceptible-atlantic-salmon-salmo-salar
#1
Phillip Dettleff, Thomas Moen, Nina Santi, Victor Martinez
The infectious salmon anemia virus (ISAv) produces a systemic infection in salmonids, causing large losses in salmon production. However, little is known regarding the mechanisms exerting disease resistance. In this paper, we perform an RNA-seq analysis in Atlantic salmon challenged with ISAv (using individuals coming from families that were highly susceptible or highly resistant to ISAv infection). We evaluated the differential expression of both host and ISAv genes in a target organ for the virus, i.e. the spleen...
January 4, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28060917/a-novel-mouse-segmentation-method-based-on-dynamic-contrast-enhanced-micro-ct-images
#2
Dongmei Yan, Zhihong Zhang, Qingming Luo, Xiaoquan Yang
With the development of hybrid imaging scanners, micro-CT is widely used in locating abnormalities, studying drug metabolism, and providing structural priors to aid image reconstruction in functional imaging. Due to the low contrast of soft tissues, segmentation of soft tissue organs from mouse micro-CT images is a challenging problem. In this paper, we propose a mouse segmentation scheme based on dynamic contrast enhanced micro-CT images. With a homemade fast scanning micro-CT scanner, dynamic contrast enhanced images were acquired before and after injection of non-ionic iodinated contrast agents (iohexol)...
2017: PloS One
https://www.readbyqxmd.com/read/28046606/tu-ab-bra-01-abdominal-synthetic-ct-generation-in-support-of-liver-sbrt-dose-calculation
#3
J S Bredfeldt, L Liu, M Feng, Y Cao, J Balter
PURPOSE: To demonstrate and validate a technique for generating MRI-derived synthetic CT volumes (MRCTs) in support of adaptive liver SBRT. METHODS: Under IRB approval, ten hepatocellular carcinoma patients were scanned using a single MR sequence (T1 Dixon-VIBE), yielding inherently-registered water, fat, and T1-weighted images. Air-containing voxels were identified by intensity thresholding. The envelope of the anterior vertebral bodies was segmented from the fat image by fitting a shape model to vertebral body candidate voxels, then using level sets to expand the contour outward...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28018701/metastatic-inflammatory-myofibroblastic-tumor-of-the-spleen-a-case-report-and-review-of-the-literature
#4
Luca Koechlin, Andreas Zettl, Dieter Koeberle, Markus von Flüe, Martin Bolli
Introduction. Inflammatory myofibroblastic tumors (IMT) of the spleen are rare neoplasms and only little is known about the origin and behavior of these tumors. Here we report the case of a 37-year-old woman with an atypical spindle cell neoplasm showing features strongly suggesting an IMT of the spleen with hepatic metastasis. Methods. A 37-year-old patient had been complaining about pain in the left upper abdomen for the last two months. A CT scan revealed a tumor mass in her spleen and liver. After complete staging, a splenectomy and atypical liver resection of segments VII and VIII were performed...
2016: Case Reports in Surgery
https://www.readbyqxmd.com/read/27982440/disseminated-infection-due-to-exophiala-pisciphila-in-cardinal-tetra-paracheirodon-axelrodi
#5
J Řehulka, M Kolařík, V Hubka
Cardinal tetra, Paracheirodon axelrodi (Schultz, 1956), kept in an ornamental tank, was found to be affected by severe invasive mycosis. Externally, the disease manifested as abdominal swelling, and internally, the anterior part of the intestine was extremely bloated with abundant dematiaceous septate hyphae and an accumulation of fluid. Histopathologically, a granulomatous inflammatory response was observed in the intestine wall, kidney and spleen. We assume that the mycotic agent was primarily deposited in the intestine and was then gradually disseminated to the other organs...
December 16, 2016: Journal of Fish Diseases
https://www.readbyqxmd.com/read/27955619/hierarchical-imaging-a-new-concept-for-targeted-imaging-of-large-volumes-from-cells-to-tissues
#6
Irene Wacker, Waldemar Spomer, Andreas Hofmann, Marlene Thaler, Stefan Hillmer, Ulrich Gengenbach, Rasmus R Schröder
BACKGROUND: Imaging large volumes such as entire cells or small model organisms at nanoscale resolution seemed an unrealistic, rather tedious task so far. Now, technical advances have lead to several electron microscopy (EM) large volume imaging techniques. One is array tomography, where ribbons of ultrathin serial sections are deposited on solid substrates like silicon wafers or glass coverslips. RESULTS: To ensure reliable retrieval of multiple ribbons from the boat of a diamond knife we introduce a substrate holder with 7 axes of translation or rotation specifically designed for that purpose...
December 12, 2016: BMC Cell Biology
https://www.readbyqxmd.com/read/27894962/clinico-pathology-and-hemato-biochemistry-responses-in-buffaloes-infected-with-pasteurella-multocida-type-b-2-immunogen-outer-membrane-protein
#7
Eric Lim Teik Chung, Faez Firdaus Jesse Abdullah, Ali Dhiaa Marza, Wessam Monther Mohammed Saleh, Hayder Hamzah Ibrahim, Yusuf Abba, Mohd Zamri-Saad, Abd Wahid Haron, Abdul Aziz Saharee, Mohd Azmi Mohd Lila, Mohd Jefri Norsidin
The aim of this study was to investigate the clinico-pathology and haemato-biochemistry alterations in buffaloes inoculated with Pasteurella multocida type B:2 immunogen outer membrane protein via subcutaneous and oral routes. Nine buffalo heifers were divided equally into 3 treatment groups. Group 1 was inoculated orally with 10 mL of phosphate buffer saline (PBS); Group 2 and 3 were inoculated with 10 mL of outer membrane protein broth subcutaneously and orally respectively. Group 2 buffaloes showed typical haemorrhagic septicaemia clinical signs and were only able to survive for 72 h of the experiment...
January 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/27885540/automatic-abdominal-multi-organ-segmentation-using-deep-convolutional-neural-network-and-time-implicit-level-sets
#8
Peijun Hu, Fa Wu, Jialin Peng, Yuanyuan Bao, Feng Chen, Dexing Kong
PURPOSE: Multi-organ segmentation from CT images is an essential step for computer-aided diagnosis and surgery planning. However, manual delineation of the organs by radiologists is tedious, time-consuming and poorly reproducible. Therefore, we propose a fully automatic method for the segmentation of multiple organs from three-dimensional abdominal CT images. METHODS: The proposed method employs deep fully convolutional neural networks (CNNs) for organ detection and segmentation, which is further refined by a time-implicit multi-phase evolution method...
November 24, 2016: International Journal of Computer Assisted Radiology and Surgery
https://www.readbyqxmd.com/read/27872541/splenic-metastasis-from-cancer-of-uterine-cervix-a-rare-case
#9
Jagannath Dixit, Noor Mohammed, Preethi Shetty
Cancer of uterine cervix is one of the most common malignancies in women. Metastatic recurrence of cervical cancer in spleen is rare, mostly seen in autopsies. Splenic metastasis from cervical cancer in a living patient without disseminated metastases is rarer. Here, we present the case of a lady aged 46 years with cervical cancer-squamous cell carcinoma-who had undergone radical hysterectomy and postoperative 50 Gy of intensity modulated radiation therapy in 25 fractions and 50 mg of weekly cisplatin for 6 weeks...
December 2016: Indian Journal of Surgical Oncology
https://www.readbyqxmd.com/read/27861351/small-amounts-of-tissue-preserve-pancreatic-function-long-term-follow-up-study-of-middle-segment-preserving-pancreatectomy
#10
Zipeng Lu, Jie Yin, Jishu Wei, Cuncai Dai, Junli Wu, Wentao Gao, Qing Xu, Hao Dai, Qiang Li, Feng Guo, Jianmin Chen, Chunhua Xi, Pengfei Wu, Kai Zhang, Kuirong Jiang, Yi Miao
Middle-segment preserving pancreatectomy (MPP) is a novel procedure for treating multifocal lesions of the pancreas while preserving pancreatic function. However, long-term pancreatic function after this procedure remains unclear.The aims of this current study are to investigate short- and long-term outcomes, especially long-term pancreatic endocrine function, after MPP.From September 2011 to December 2015, 7 patients underwent MPP in our institution, and 5 cases with long-term outcomes were further analyzed in a retrospective manner...
November 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27858212/hepatosplenic-volumetric-assessment-at-mdct-for-staging-liver-fibrosis
#11
Perry J Pickhardt, Kyle Malecki, Oliver F Hunt, Claire Beaumont, John Kloke, Timothy J Ziemlewicz, Meghan G Lubner
PURPOSE: To investigate hepatosplenic volumetry at MDCT for non-invasive prediction of hepatic fibrosis. METHODS: Hepatosplenic volume analysis in 624 patients (mean age, 48.8 years; 311 M/313 F) at MDCT was performed using dedicated software and compared against pathological fibrosis stage (F0 = 374; F1 = 48; F2 = 40; F3 = 65; F4 = 97). The liver segmental volume ratio (LSVR) was defined by Couinaud segments I-III over segments IV-VIII. All pre-cirrhotic fibrosis stages (METAVIR F1-F3) were based on liver biopsy within 1 year of MDCT...
November 17, 2016: European Radiology
https://www.readbyqxmd.com/read/27846664/laparoscopic-splenectomy-in-children-a-modified-technique-with-joint-sealing-of-segmental-splenic-arteries-and-veins-favorably-compared-with-the-classic-technique
#12
Sara Silvaroli, Marianne De Montalembert, Frédérique Sauvat, Valentine Brousse, Sabine Sarnacki, Sabine Irtan
Introduction Splenectomy in children is nowadays widely performed by laparoscopy, the major risk being operative hemorrhage. We hereby compare the results of two different techniques of vascular control. Materials and Methods We retrospectively reviewed all patients undergoing a laparoscopic splenectomy (LS) for hematologic disease at a single institution from 2004 to 2013. LS was performed by a lateral approach. The 10-mm optic port was placed in the umbilicus and two 5-mm working ports were placed in the left lower quadrant and in the right upper quadrant near the midline...
November 15, 2016: European Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/27806771/-surgical-treatment-of-pancreatic-cystic-neoplasms-follow-up-or-surgical-intervention
#13
X D Tian, Y M Yang
Pancreatic cystic neoplasm(PCN) are common and increasingly detected in recent years including serous cystic neoplasms, mucinous cystic neoplasms, intraductal papillary mucinous neoplasms, solid pseudopapillary neoplasms and cystic pancreatic neuroendocrine tumors.Some of PCN have a low risk of malignancy while others have a high risk and need interventions, even in the same type of cystic neoplasms. The management of PCN requires risk stratification for malignant potential, and clinicians should have a systematic approach for establishing a diagnosis and determining which patients require surgical treatment...
November 1, 2016: Zhonghua Wai Ke za Zhi [Chinese Journal of Surgery]
https://www.readbyqxmd.com/read/27778153/phenotypic-characterization-of-a-novel-ho-1-depletion-model-in-the-rat
#14
Vassilios Atsaves, Maria G Detsika, Elpida Poulaki, Hara Gakiopoulou, Elias A Lianos
Although the protective role of HO-1 induction in various forms of kidney disease is well established, mechanisms other than heme catabolism to biliverdin, bilirubin and carbon monoxide have recently been identified. Unraveling these mechanisms requires the generation of appropriate animal models. The present study describes the generation of a HO-1 deficient Hmox1 (-/-) rat model and characterizes its renal and extrarenal phenotype. Hmox1 (-/-) rats had growth retardation and splenomegaly compared to their Hmox1 (+/+) littermates...
February 2017: Transgenic Research
https://www.readbyqxmd.com/read/27777473/changes-seen-on-computed-tomography-of-the-chest-in-mildly-symptomatic-adult-patients-with-sickle-cell-disease
#15
Ursula David Alves, Agnaldo José Lopes, Maria Christina Paixão Maioli, Andrea Ribeiro Soares, Pedro Lopes de Melo, Roberto Mogami
OBJECTIVE: To describe and quantify the main changes seen on computed tomography of the chest in mildly symptomatic adult patients with sickle cell disease, as well as to evaluate the radiologist accuracy in determining the type of hemoglobinopathy. MATERIALS AND METHODS: A prospective study involving 44 adult patients with sickle cell disease who underwent inspiration and expiration computed tomography of the chest. The frequency of tomography findings and the extent of involvement are reported...
July 2016: Radiologia Brasileira
https://www.readbyqxmd.com/read/27726419/effects-of-in-ovo-supplementation-of-probiotics-on-performance-and-immunocompetence-of-broiler-chicks-to-an-eimeria-challenge
#16
C M Pender, S Kim, T D Potter, M M Ritzi, M Young, R A Dalloul
Coccidiosis is regarded as the parasitic disease with the greatest economic impact on the poultry industry due to reduced performance and increased mortality. A study was conducted to investigate the effects of in ovo administration of probiotics on hatchability, performance, immune organ weights, and lesion scores in broiler chicks during a mixed Eimeria infection. At embryonic day 18, 210 eggs were injected with either sterile water or 1×10(6) cfu probiotic bacteria. On day 3 post-hatch, half of the chicks from each treatment group were challenged with a mixed inoculum of Eimeria acervulina, Eimeria maxima and Eimeria tenella...
November 30, 2016: Beneficial Microbes
https://www.readbyqxmd.com/read/27698167/hernia-of-the-swim-bladder-aerocystocele-with-concurrent-mycotic-granulomatous-inflammation-and-swim-bladder-carcinoma-in-a-wild-mullet-mugil-cephalus
#17
Rubina Sirri, Carlo Bianco, Gaetano Zuccaro, Maria Elena Turba, Luciana Mandrioli
We describe a hernia of the swim bladder, with a concurrent mycotic granulomatous inflammation, and carcinoma of the swim bladder in a wild mullet (Mugil cephalus) referred for an exophytic dorsal mass. Grossly, the mass was white, soft, and composed of multiple cystic gas-containing chambers connected by a funnel-shaped tissue segment to the coelomic swim bladder. Histologically, the mass was characterized by cysts of variable size, multifocally contiguous with the subepithelial rete mirabile, supported by abundant fibrous tissue...
November 2016: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/27663650/rib-fractures-and-their-association-with-solid-organ-injury-higher-rib-fractures-have-greater-significance-for-solid-organ-injury-screening
#18
Jack W Rostas, Timothy B Lively, Sidney B Brevard, Jon D Simmons, Mohammad A Frotan, Richard P Gonzalez
BACKGROUND: The purpose of this study was to identify patients with rib injuries who were at risk for solid organ injury. METHODS: A retrospective chart review was performed of all blunt trauma patients with rib fractures during the period from July 2007 to July 2012. Data were analyzed for association of rib fractures and solid organ injury. RESULTS: In all, 1,103 rib fracture patients were identified; 142 patients had liver injuries with 109 (77%) associated right rib fractures...
August 28, 2016: American Journal of Surgery
https://www.readbyqxmd.com/read/27660169/nonfluorodeoxyglucose-avid-persistent-splenomegaly-at-time-of-transplantation-delays-neutrophil-and-platelets-engraftment-without-affecting-survival-in-patients-with-lymphomas-undergoing-allogeneic-hematopoietic-cell-transplantation
#19
Farhad Khimani, Daniel K Jeong, Branko Miladinovic, Taiga Nishihori, Ernesto Ayala, Frederick Locke, Asmita Mishra, Julio Chavez, Bijal Shah, Kenneth Gage, Mohamed A Kharfan-Dabaja
It is unclear if persistent splenomegaly in the presence of a negative positron emission tomography (PET) scan before allogeneic hematopoietic cell transplantation (HCT) influences post-transplantation outcomes in patients with lymphoma. We retrospectively reviewed records of 152 patients who underwent allogeneic HCT for various lymphomas. Centralized review of pretransplantation computed tomography (CT) and PET images was performed. Spleen volume (SV) was measured using the freehand volume segmentation tool in AW Workstation software (General Electric, Waukesha, WI)...
September 19, 2016: Biology of Blood and Marrow Transplantation
https://www.readbyqxmd.com/read/27541630/3d-statistical-shape-models-incorporating-landmark-wise-random-regression-forests-for-omni-directional-landmark-detection
#20
Tobias Norajitra, Klaus H Maier-Hein
3D Statistical Shape Models (3D-SSM) are widely used for medical image segmentation. However, during segmentation, they typically perform a very limited unidirectional search for suitable landmark positions in the image, relying on weak learners or use-case specific appearance models that solely take local image information into account. As a consequence, segmentation errors arise, and results in general depend on the accuracy of a previous model initialization. Furthermore, these methods become subject to a tedious and use-case dependent parameter tuning in order to obtain optimized results...
January 2017: IEEE Transactions on Medical Imaging
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