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https://www.readbyqxmd.com/read/29784000/the-homing-of-bone-marrow-stem-cells-is-differentially-activated-in-ischemic-and-valvular-heart-diseases-and-influenced-by-beta-blockers
#1
Melissa Kristocheck, Lucinara D Dias, Carine Ghem, Bruna Eibel, Renato A K Kalil, Melissa M Markoski
BACKGROUND: Cell homing is the mechanism by which an injury releases signaling molecules that cause recruitment, proliferation, migration and differentiation of progenitor cells. Stromal derived factor-1 (SDF-1) and its receptor CXCR4 are key molecules involved in homing and little is known about their activation in cardiopathies. Here, we assessed the homing activation status of bone marrow cells (BMC) concerning the SDF-1 and CXCR4 expression in ischemic (IHD) and valvular (VHD) heart diseases...
May 21, 2018: Journal of Translational Medicine
https://www.readbyqxmd.com/read/29783047/stroma-cell-derived-factor-1-and-connexins-37-and-43-are-preserved-after-vitrification-and-in-vitro-culture-of-goat-ovarian-cortex
#2
Nathalie Jiatsa Donfack, Kele Amaral Alves, Benner Geraldo Alves, Rebeca Magalhães Pedrosa Rocha, Jamily Bezzera Bruno, Marcelo Bertolini, Regiane Rodrigues Dos Santos, Sheyla Farhaydes Souza Domingues, José Ricardo De Figueiredo, Johan Smitz, Ana Paula Ribeiro Rodrigues
This study aimed to evaluate the follicular morphology and development (follicular activation, cell proliferation, and hormone production), as well as the distribution pattern of Connexins 37 and 43 and SDF-1α after vitrification and in vitro culture of goat ovarian tissue. The study involved four experimental groups: fresh control, vitrified control, fresh culture and vitrified culture. The ovarian fragments were vitrified by a solid surface technique using the Ovarian Tissue Cryosystem and subsequently in vitro cultured for 7 days...
May 12, 2018: Theriogenology
https://www.readbyqxmd.com/read/29779212/periodontal-regeneration-via-chemo-attractive-constructs
#3
Xinjie Cai, Fang Yang, X Frank Walboomers, Yining Wang, John A Jansen, Jeroen J J P van den Beucken, Adelina S Plachokova
AIM: Chemo-attractants, such as stromal cell-derived factor-1α (SDF-1α), can offer an advantage for periodontal regeneration by recruiting the patient's own stem cells to stimulate self-repair. We here developed a chemo-attractive construct for periodontal regeneration using SDF-1α and evaluated its efficacy in vivo. MATERIALS AND METHODS: SDF-1α was loaded on gelatin sponge and tested in vitro for SDF-1α release. Subsequently, SDF-1α constructs were implanted into rat periodontal defects for 1 and 6 weeks, with unloaded materials and empty defects as controls...
May 19, 2018: Journal of Clinical Periodontology
https://www.readbyqxmd.com/read/29777958/sssupramolecular-poly-acrylic-acid-f127-hydrogel-with-hydration-controlled-nitric-oxide-release-for-enhancing-wound-healing
#4
Mathilde Champeau, Valéria Póvoa, Lucas Militão, Flávia Cabrini, Guilherme F Picheth, Florian Meneau, Carlos P Jara, Eliana P de Araujo, Marcelo G de Oliveira
Topical nitric oxide (NO) delivery has been shown to accelerate wound healing. However, delivering NO to wounds at appropriate rates and doses requires new biomaterial-based strategies. Here, we describe the development of supramolecular interpolymer complex hydrogels comprising PEO-PPO-PEO (F127) micelles embedded in a poly(acrylic acid) (PAA) matrix, with S-nitrosoglutathione (GSNO) molecules dissolved in the hydrophilic domain. We show that PAA:F127/GSNO hydrogels start releasing NO upon hydration at rates controlled by their rates of water absorption...
May 16, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29776406/the-effect-of-dpp-4-inhibition-to-improve-functional-outcome-after-stroke-is-mediated-by-the-sdf-1%C3%AE-cxcr4-pathway
#5
Fausto Chiazza, Harald Tammen, Hiranya Pintana, Grazyna Lietzau, Massimo Collino, Thomas Nyström, Thomas Klein, Vladimer Darsalia, Cesare Patrone
BACKGROUND: Dipeptidyl peptidase-4 (DPP-4) inhibitors (gliptins) are approved drugs for the treatment of hyperglycemia in patients with type 2 diabetes. These effects are mainly mediated by inhibiting endogenous glucagon-like peptide-1 (GLP-1) cleavage. Interestingly, gliptins can also improve stroke outcome in rodents independently from GLP1. However, the underlying mechanisms are unknown. Stromal cell-derived factor-1α (SDF-1α) is a DPP-4 substrate and CXCR4 agonist promoting beneficial effects in injured brains...
May 19, 2018: Cardiovascular Diabetology
https://www.readbyqxmd.com/read/29771429/the-relationship-between-number-and-function-of-epcs-and-concentration-of-vegf165-and-sdf-1-in-coronary-artery-spasm
#6
Z-Q Sheng, Y-F Li, K-L Zheng, H-H Lu, J Xie, H Wu, B Xu
OBJECTIVE: We aimed at investigating the functions of endothelial progenitor cells (EPCs) in the pathophysiology of coronary artery spasm (CAS) and coronary artery disease (CAD), as well as to evaluate the correlation of these diseases with the number and function of EPCs, the plasma concentration of vascular endothelial growth factor 165 (VEGF165) and stromal cell-derived factor 1 (SDF-1). PATIENTS AND METHODS: Participants were recruited into three groups, CAS, CAD and the control...
May 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29768975/arresting-dentine-caries-with-silver-diamine-fluoride-what-s-behind-it
#7
M L Mei, E C M Lo, C H Chu
Unlike other fluoride-based caries preventive agents, silver diamine fluoride (SDF) can simultaneously prevent and arrest coronal and root dentine caries. The profound clinical success of SDF has drawn many clinicians and researchers to study the mechanism of SDF in arresting dentine caries. This critical review discusses how silver and fluoride contribute to caries arrest, in terms of their effects on bacteria as well as on the mineral and organic content of dentine. Silver interacts with bacterial cell membrane and bacterial enzymes, which can inhibit bacterial growth...
May 1, 2018: Journal of Dental Research
https://www.readbyqxmd.com/read/29767411/mek1-2-inhibition-by-binimetinib-is-effective-as-a-single-agent-and-potentiates-the-actions-of-venetoclax-and-abt-737-under-conditions-that-mimic-the-chronic-lymphocytic-leukaemia-cll-tumour-microenvironment
#8
Kyle Crassini, Yandong Shen, William S Stevenson, Richard Christopherson, Chris Ward, Stephen P Mulligan, O Giles Best
The survival and proliferation of chronic lymphocytic leukaemia (CLL) cells is driven by multiple signalling pathways, including those mediated by the B cell, Toll-like and chemokine receptors. Many of these pathways converge on the same signalling molecules, including those involved in the Raf-1/MEK/Erk1/2-MAPK pathway. We investigated the effects of the MEK1/2 (also termed MAP2K1/2) inhibitor, binimetinib, against CLL cells cultured under conditions that mimic aspects of the tumour microenvironment. Binimetinib blocked CLL cell survival induced by stroma-conditioned media and phorbol myristylate (PMA)...
May 16, 2018: British Journal of Haematology
https://www.readbyqxmd.com/read/29766675/interactions-between-monocytes-mesenchymal-stem-cells-and-implants-evaluated-using-flow-cytometry-and-gene-expression
#9
Maria Lennerås, Karin Ekström, Forugh Vazirisani, Furqan A Shah, Katarina Junevik, Peter Thomsen, Omar Omar
Monocytes and mesenchymal stem cells (MSC) are evident at the implants during early healing. However, when coexisting, their interactions at different implants have not been determined. This study uses an in vitro system, consisting of mono- and direct co-cultures of monocytes and MSC on screw-shaped machined and oxidized titanium implants in combination with scanning electron microscopy, ELISA, flow cytometry, cell sorting, and qPCR. The cell-specific adhesion and gene expression of monocytes and MSC was determined...
May 15, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29763775/glycosaminoglycan-based-hybrid-hydrogel-encapsulated-with-polyelectrolyte-complex-nanoparticles-for-endogenous-stem-cell-regulation-in-central-nervous-system-regeneration
#10
Wei-Hong Jian, Huan-Chih Wang, Chen-Hsiang Kuan, Ming-Hong Chen, Hsi-Chin Wu, Jui-Sheng Sun, Tzu-Wei Wang
The poor regenerative capability of stem cell transplantation in the central nervous system limits their therapeutic efficacy in brain injuries. The sustained inflammatory response, lack of structural support, and trophic factors deficiency restrain the integration and long-term survival of stem cells. Instead of exogenous stem cell therapy, here we described the synthesis of nanohybrid hydrogel containing sulfated glycosaminoglycan-based polyelectrolyte complex nanoparticles (PCN) to mimic the brain extracellular matrix and control the delivery of stromal-derived factor-1α (SDF-1α) and basic fibroblast factor (bFGF) in response to matrix metalloproteinase (MMP) for recruiting endogenous neural stem cells (NSC) and regulating their cellular fate...
May 8, 2018: Biomaterials
https://www.readbyqxmd.com/read/29758935/blood-urea-nitrogen-bun-independently-predicts-mortality-in-critically-ill-patients-admitted-to-icu-a-multicenter-study
#11
Bernhard Wernly, Michael Lichtenauer, Namkje A R Vellinga, E Christiaan Boerma, Can Ince, Malte Kelm, Christian Jung
BACKGROUND AND PURPOSE: The Microcirculatory Shock Occurrence in Acutely Ill Patients (micro-SOAP) study investigated associations of microcirculation and mortality. Risk stratification in critically ill patients is of utmost interest. Established score such as APACHE2 (Acute Physiology And Chronic Health Evaluation 2) are relatively complex and might therefore be of limited use. Blood urea nitrogen (BUN) was described to be associated with mortality in various diseases. We therefore aimed (i) to evaluate BUN for prediction of mortality in a cohort of critically ill patients and (ii) to investigate associations of BUN with microcirculation...
2018: Clinical Hemorheology and Microcirculation
https://www.readbyqxmd.com/read/29758548/stromal-cell-derived-factor-sdf-1-alpha-overexpression-promotes-bone-regeneration-by-osteogenesis-and-angiogenesis-in-osteonecrosis-of-the-femoral-head
#12
Fan Yang, Feng Xue, Junjie Guan, Zeng Zhang, Jimin Yin, Qingling Kang
BACKGROUND/AIMS: Osteonecrosis of the femoral head (ONFH) is a devastating orthopedic disease. Previous studies suggested that stromal-cell-derived factor (SDF)-1 was involved in osteogenesis and angiogenesis. However, whether SDF-1 potentiates the angiogenesis and osteogenesis of bone marrow-derived stromal stem cells (BMSCs) in ONFH is not clear. METHODS: BMSCs were transfected with green fluorescent protein (GFP) or the fusion gene encoding GFP and SDF-1α, and transgenic efficacy was monitored by immunofluorescence...
May 7, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29758486/poly-i-c-and-lps-induce-distinct-immune-responses-by-ovarian-stromal-fibroblasts
#13
Mickey V Patel, Zheng Shen, Charles R Wira
Despite its anatomical location, the ovary is a site of pathogen exposure in the human female reproductive tract (FRT). However, the role of ovarian stromal fibroblasts in immune protection is unclear. We generated a population of ovarian stromal fibroblasts derived from normal human ovaries that expressed the pattern recognition receptors TLR3, TLR4, RIG-I, & MDA5. Poly (I:C) and LPS, respective mimics of viral and bacterial infections, selectively upregulated antiviral gene expression and secretion of chemokines and antimicrobials...
May 9, 2018: Journal of Reproductive Immunology
https://www.readbyqxmd.com/read/29752864/%C3%AE-blockers-interfere-with-cell-homing-receptors-and-regulatory-proteins-in-a-model-of-spontaneously-hypertensive-rats
#14
Bruna Eibel, Melissa Kristochek, Thiago R Peres, Lucinara D Dias, Daniela R Dartora, Karina R Casali, Renato A K Kalil, Alexandre M Lehnen, Maria Claudia Irigoyen, Melissa M Markoski
AIM: To examine the interference of β-blockers with the chemokine stromal cell-derived factor-1 (SDF-1) found in cell homing receptors, C-X-C chemokine receptor type 4 (CXCR-4) and CXCR-7 and regulatory proteins of homing pathways, we administered atenolol, carvedilol, metoprolol and propranolol for 30 days using an orogastric tube to hypertensive rats. METHOD: We collected blood samples before and after treatment and quantified the levels of SDF-1 with enzyme linked immunosorbent assay (ELISA)...
May 12, 2018: Cardiovascular Therapeutics
https://www.readbyqxmd.com/read/29752300/intramyocardial-angiogenetic-stem-cells-and-epicardial-erythropoietin-save-the-acute-ischemic-heart
#15
Christian Klopsch, Anna Skorska, Marion Ludwig, Heiko Lemcke, Gabriela Maass, Ralf Gaebel, Martin Beyer, Cornelia Lux, Anita Toelk, Karina Müller, Christian Maschmeier, Sarah Rohde, Petra Mela, Brigitte Müller-Hilke, Stefan Jockenhoevel, Brigitte Vollmar, Robert Jaster, Robert David, Gustav Steinhoff
Ischemic heart failure still displays the highest mortality. An early boost of intracardiac regenerative key mechanisms and angiogenetic niche signaling in cardiac mesenchymal stem cells (MSCs) could improve myocardial infarction (MI) healing. Epicardial erythropoietin (EPO, 300U kg-1 ) was compared with intraperitoneal and intramyocardial EPO treatments after acute MI in rats (n=156). Real-time PCR and confocal microscopy revealed epicardial EPO treatment enhanced intracardiac regenerative key indicators (SDF-1, CXCR-4, CD34, Bcl-2, Cyclin D1, Cdc2, MMP2), induced TGF-β/WNT signaling in intramyocardial MSC niches through direct activation of AKT, upregulations of upstream signals FOS and Fzd7 and augmented intracardiac mesenchymal proliferation 24 hours after MI...
May 10, 2018: Disease Models & Mechanisms
https://www.readbyqxmd.com/read/29752108/surface-decorated-hydroxyapatite-scaffold-with-on-demand-delivery-of-dexamethasone-and-stromal-cell-derived-factor-1-for-enhanced-osteogenesis
#16
Bingjun Zhang, Hongyu Li, Lei He, Zhiwei Han, Teng Zhou, Wei Zhi, Xiaobo Lu, Xiong Lu, Jie Weng
In the process of bone regeneration, relatively early biological events including inflammatory response, angiogenesis, or stem cell homing, help the accompanying target actions of cell differentiation and calcification. Herein, we proposed a novel cell-guided tissue engineering system based on a surface-functionalized porous hydroxyapatite (HA) scaffolds with the ability to recruit cells and accelerate the differentiation of them along the osteoblastic lineage for optimizing large-sized bone defect repair. Inspired by microstructural properties of natural bone, HA scaffolds similar to the trabecular bone structure were prepared via a sugar sphere leaching technique, in which the inter-pore opening size was controllable...
August 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/29749473/effect-of-a-novel-orally-bioavailable-cxcr4-inhibitor-amd070-on-the-metastasis-of-oral-cancer-cells
#17
Daisuke Uchida, Nobuyuki Kuribayashi, Makoto Kinouchi, Yuta Sawatani, Michiko Shimura, Toshimitsu Mori, Tomonori Hasegawa, Youji Miyamoto, Hitoshi Kawamata
We have previously demonstrated that the stromal cell‑derived factor (SDF‑1)/CXCR4 system is involved in the metastasis of head and neck cancer. Additionally, it has been revealed that the blockade of CXCR4 by subcutaneous daily injection with AMD3100, a CXCR4 antagonist, may be effective in preventing metastasis in CXCR4‑related head and neck cancer. Recent investigations have suggested that AMD070, a novel orally bioavailable inhibitor of CXCR4, may be minimally invasive compared with AMD3100. In the present study, we examined the effect of AMD070 on metastasis induced by the SDF‑1/CXCR4 axis in B88‑SDF‑1 oral cancer cells, which express high levels of SDF‑1 and CXCR4...
April 25, 2018: Oncology Reports
https://www.readbyqxmd.com/read/29745251/amd3100-ameliorates-cigarette-smoke-induced-emphysema-like-manifestations-in-mice
#18
Daria Barwinska, Houssam Oueini, Christophe Poirier, Marjorie E Albrecht, Natalia V Bogatcheva, Matthew J Justice, Jacob Saliba, Kelly S Schweitzer, Hal E Broxmeyer, Keith L March, Irina Petrache
We have shown that cigarette smoke (CS)-induced pulmonary emphysema-like manifestations are preceded by marked suppression of the number and function of bone marrow hematopoietic progenitor cells (HPC). To investigate if a limited availability of HPC may contribute to CS-induced lung injury, we used an FDA-approved antagonist of the interactions of SDF-1 with its receptor CXCR4 to promote intermittent HPC mobilization and tested its ability to limit emphysema-like injury following chronic CS. We administered AMD3100 (5mg/kg) to mice during a chronic CS exposure protocol of up to 24 weeks...
May 10, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/29740450/a-novel-three-dimensional-immune-oncology-model-for-high-throughput-testing-of-tumoricidal-activity
#19
Hilary Sherman, Hannah J Gitschier, Ann E Rossi
The latest advancements in oncology research are focused on autologous immune cell therapy. However, the effectiveness of this type of immunotherapy for cancer remediation is not equivalent for all patients or cancer types. This suggests the need for better preclinical screening models that more closely recapitulate in vivo tumor biology. The established method for investigating tumoricidal activity of immunotherapies has been study of two-dimensional (2D) monolayer cultures of immortalized cancer cell lines or primary tumor cells in standard tissue culture vessels...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29738669/cypreact-a-software-tool-for-in-silico-reactant-prediction-for-human-cytochrome-p450-enzymes
#20
Siyang Tian, Yannick Djoumbou, Russ Greiner, David S Wishart
In silico metabolism prediction requires first predicting whether a specific molecule will interact with one or more specific metabolizing enzymes, then predicting the result of each enzymatic reaction. Here, we provide a computational tool, CypReact, for performing this first task of reactant prediction. Specically, CypReact takes as input an arbitrary molecule (specied as a SMILES string or a standard SDF file), and any one of the nine of the most important human cytochrome P450 (CYP450) enzymes -- CYP1A2, CYP2A6, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, CYP2E1 or CYP3A4 -- and accurately predicts whether the query molecule will react with that given CYP450 enzyme...
May 8, 2018: Journal of Chemical Information and Modeling
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