keyword
MENU ▼
Read by QxMD icon Read
search

Tear cytokines

keyword
https://www.readbyqxmd.com/read/29914680/survey-of-cytokines-in-normal-canine-tears-by-multiplex-analysis-a-pilot-study
#1
Paoul S Martinez, Eric S Storey, Cherie M Pucheu-Haston
OBJECTIVES: The purpose of this pilot study was to use a multiplexed assay to measure cytokines in normal stimulated canine tears. METHODS: 25 healthy dogs were included in the study. Stimulated tears were collected in capillary tubes from the right (OD) and left (OS) eyes and stored at -80 °C until batch sample analysis was performed. The samples were analyzed utilizing Luminex® canine-validated multiplex beads on a Bio-Rad multiplex analyzer for IL-2, IL-6, IL-7, IL-8, IL-10, TNF-α, and IFN-γ...
July 2018: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/29874670/il-1%C3%AE-and-mmp-9-tear-levels-of-patients-with-active-ocular-rosacea-before-and-after-treatment-with-systemic-azithromycin-or-doxycycline
#2
Lorena Lam-Franco, Yocanxochitl Perfecto-Avalos, Beatriz E Patiño-Ramírez, Alejandro Rodríguez García
AIMS: The purpose of this paper was to determine the lacrimal concentration of IL-1α and MMP-9 in patients with active ocular rosacea before and after systemic treatment with azithromycin or doxycycline. METHODS: After 4 weeks of therapy with azithromycin (500 mg/day, 3 days a week PO) or doxycycline (200 mg/day PO), lacrimal samples were analyzed using an enzyme-linked immunosorbent assay multiplex. RESULTS: There was a significant difference between baseline IL-1α (37...
June 6, 2018: Ophthalmic Research
https://www.readbyqxmd.com/read/29849773/extended-low-oxygen-transmissibility-contact-lens-use-induces-alterations-in-the-concentration-of-proinflammatory-cytokines-enzymes-and-electrolytes-in-tear-fluid
#3
Luis Fernando Barba Gallardo, Martín Humberto Muñoz Ortega, Javier Ventura Juarez, Liseth Rubí Aldaba Muruato, Esperanza Sánchez Alemán, Eduardo Emmanuel Valdez Morales, Sugela Susana Blancas Zugarazo, Jose Rafael Villafan Bernal
Prolonged and continuous use of contact lenses for as long as 3 or 4 weeks is common in Mexico due to the low socioeconomic status, poor patient education and self-neglect. Furthermore, wearing contact lenses with low oxygen permeability is common due to their low cost. Thus, patients seek ophthalmologic evaluation due to signs and symptoms of overuse such as red eye, discomfort and tearing. In the present study, the effect of wearing soft contact lenses with a low oxygen permeability on the tear fluid composition after 1 day, 1 week and 1 month without removing them was examined...
May 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29803660/level-of-tear-cytokines-in-population-level-participants-and-correlation-with-clinical-features
#4
L Tong, T Y Wong, Y Cheng
AIMS: Tear cytokine levels indicate severity of ocular surface inflammation. Previous reports of cytokine concentrations were based on hospital-based studies or non-Chinese populations. We determine the range of tear concentration of cytokines in a representative adult Chinese population. METHODS: Thirty-nine participants were recruited from a population-based study of Chinese adults in Singapore, and standardized clinical ocular surface/eyelid features evaluated...
May 23, 2018: Cytokine
https://www.readbyqxmd.com/read/29801153/the-level-of-inflammatory-tear-cytokines-is-elevated-in-congenital-aniridia-and-associated-with-meibomian-gland-dysfunction
#5
Erlend C S Landsend, Øygunn A Utheim, Hilde R Pedersen, Hans Christian D Aass, Neil Lagali, Darlene A Dartt, Rigmor C Baraas, Tor P Utheim
Purpose: To investigate the tear cytokine profile in congenital aniridia, and correlate cytokine levels with ophthalmologic findings. Methods: We examined 35 patients with aniridia and 21 healthy controls. Tear fluid was collected with Schirmer I test and capillary tubes from each eye, and the concentration of 27 inflammatory cytokines determined using multiplex bead assay. Eyes of all participants were examined with tests for dry eye disease, including evaluation of meibomian glands (meibography)...
April 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29777869/subconjunctival-dendrimer-drug-therapy-for-the-treatment-of-dry-eye-in-rabbits-with-induced-autoimmune-dacryoadenitis
#6
Hui Lin, Ying Liu, Siva P Kambhampati, Chih-Chien Hsu, Rangaramanujam M Kannan, Samuel C Yiu
PURPOSE: To investigate the efficacy of a single subconjunctival injection of dendrimer-dexamethasone to treat dry eye in a rabbit model of induced autoimmune dacryoadenitis (AID). METHOD: Dendrimer biodistribution after subconjuntival injection in AID animals was evaluated using Cy5-labelled dendrimer (D-Cy5) and confocal microscopy. Diseased animals were treated with free dexamethasone (Free-Dex), dendrimer-dexamethasone (D-Dex), or saline via a single subconjunctival injection...
May 16, 2018: Ocular Surface
https://www.readbyqxmd.com/read/29723628/tear-inflammatory-mediators-and-protein-in-eyes-of-post-allogenic-hematopoeitic-stem-cell-transplant-patients
#7
Sridevi Nair, Murugesan Vanathi, Manoranjan Mahapatra, Tullika Seth, Jasbir Kaur, T Velpandian, Alok Ravi, Jeevan Singh Titiyal, Radhika Tandon
AIM: To analyze tear cytokines levels and their correlation to ocular surface parameters in allogenic hematopoietic stem cell transplants (allo-HSCT) patients. METHODS: Prospective longitudinal study of allo-HSCT patients and controls for ocular surface evaluation (OSDI, TBUT, Schirmer's test, staining scores), tear biochemical analysi for protein, cytokines [IL-10, IL-12, IL-2, IL-4, IL-6, IL-17, interferon (IFN)-gamma, tumor necrosis factor (TNF)-alpha, VEGF], MMPs [MMP 2, 9, 7, 13, 10 and chemokine (IL-8)], & VEGF on three consecutive follow up visits (at three monthly interval) was done...
April 30, 2018: Ocular Surface
https://www.readbyqxmd.com/read/29610847/the-imbalance-of-lymphocyte-subsets-and-cytokines-potential-immunologic-insights-into-the-pathogenesis-of-chronic-dacryocystitis
#8
Xiaoxuan Yang, Lizhen Wang, Lunhao Li, Zhang Yu, Caiwen Xiao
Purpose: To explore the quantitative distributions of different lymphocyte subsets in the lacrimal sac mucosa and identify changes of Th1- and Th2-associated cytokines in the tears of patients with chronic dacryocystitis. Methods: Lacrimal sac mucosal specimens from patients with chronic dacryocystitis were analyzed. Hematoxylin-eosin staining and Masson staining were performed for pathologic analysis, and immunohistochemical staining was performed for the detection of CD3+, CD4+, CD8+, CD20+, Th1, and Th2 lymphocytes...
April 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29555477/zidovudine-protects-hyperosmolarity-stressed-human-corneal-epithelial-cells-via-antioxidant-pathway
#9
Hui Liu, Frank Gambino, Crystal Algenio, Charles Bouchard, Liang Qiao, Ping Bu, Shaozhen Zhao
Dry Eye Disease (DED) is a very common disorder that can result in severe disability and vision loss. Although the pathogenesis of DED is not fully understood, hyperosmolarity, inflammation, and tear film instability are recognized as hallmarks of DED. Recently, Nucleoside Reverse Transcriptase Inhibitors (NRTIs), a class of medication used to treat HIV, have been shown to inhibit inflammation in a mouse model of retinal atrophy. In this study, we investigated whether Zidovudine (AZT) can inhibit human corneal epithelial cell (HCEC) inflammatory responses under hyperosmotic conditions...
May 5, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29499288/nlrp3-as-a-potentially-novel-biomarker-for-the-management-of-osteoarthritis
#10
REVIEW
M J McAllister, M Chemaly, A J Eakin, D S Gibson, V E McGilligan
Osteoarthritis (OA) was previously thought of as 'wear and tear' as humans age, however there is increasing evidence to support an inflammatory theory. The nucleotide-binding and oligomerization domain-like receptor containing protein 3 (NLRP3) inflammasome has been implicated in the pathogenesis of a number of arthritic disorders, producing proinflammatory cytokines and degradative enzymes such as Interleukin-1 beta (IL-1β), Tumour necrosis factor alpha (TNF-α) and Matrix metalloproteinase-3 (MMP-3) which drive cartilage degeneration and synovial inflammation...
May 2018: Osteoarthritis and Cartilage
https://www.readbyqxmd.com/read/29481958/erratum-to-tear-cytokine-profile-of-glaucoma-patients-treated-with-preservative-free-or-preserved-latanoprost
#11
Jose M Martinez-de-la-Casa, Francisco Perez-Bartolome, Elena Urcelay, Jose L Santiago, Javier Moreno-Montanes, Pedro Arriola-Villalobos, Jose M Benitez-Del-Castillo, Julian Garcia-Feijoo
No abstract text is available yet for this article.
April 2018: Ocular Surface
https://www.readbyqxmd.com/read/29439091/evaluation-of-galectin-1-and-galectin-3-as-prospective-biomarkers-in-keratoconus
#12
Frans Eberth Costa Andrade, Joyce Luciana Covre, Lisandra Ramos, Rossen Mihaylov Hazarbassanov, Myrna Serapião Dos Santos, Mauro Campos, José Álvaro Pereira Gomes, Cristiane Damas Gil
AIMS: To evaluate the expression of β-galactoside-binding proteins galectin (Gal)-1 and Gal-3 in patients with keratoconus (KC) and postcorneal collagen cross-linking (CXL) treatment in vitro. METHODS: Tear fluid, cornea samples and conjunctival impression cytology specimens from control and KC patients were used to evaluate Gal-1 and Gal-3 expressions. Primary keratocytes were isolated by collagenase digestion from surgically removed corneas of five normal or KC human corneal buttons and cultured in Dulbecco's modified eagle medium/Ham's F12 medium supplemented with 2% fetal bovine serum...
May 2018: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/29411322/the-role-of-biologic-therapy-in-rotator-cuff-tears-and-repairs
#13
REVIEW
Michael D Charles, David R Christian, Brian J Cole
PURPOSE OF REVIEW: The purpose of this review was to establish the foundation of the major biologic adjuvants to rotator cuff repairs and review recent scientific findings. RECENT FINDINGS: Platelet-rich plasma (PRP) overall has no significant impact on functional outcomes and repair integrity, but may be more advantageous in small to medium tears. Further studies should focus on leukocyte-rich versus poor preparations and the use of PRP in patients that are high risk for repair failure...
March 2018: Current Reviews in Musculoskeletal Medicine
https://www.readbyqxmd.com/read/29364702/synovial-fluid-profile-at-the-time-of-anterior-cruciate-ligament-reconstruction-and-its-association-with-cartilage-matrix-composition-3-years-after-surgery
#14
Keiko Amano, Janet L Huebner, Thomas V Stabler, Matthew Tanaka, Charles E McCulloch, Iryna Lobach, Nancy E Lane, Virginia B Kraus, C Benjamin Ma, Xiaojuan Li
BACKGROUND: Anterior cruciate ligament tears can lead to posttraumatic osteoarthritis. In addition to biomechanical factors, changes in biochemical profiles within the knee joint after injury and anterior cruciate ligament reconstruction (ACLR) may play a role in accelerating joint degeneration. Hypothesis/Purpose: It was hypothesized that cartilage matrix composition after ACLR is associated with the degree of inflammatory response after initial injury. This study evaluated the association between the inflammatory response after injury-as indicated by cytokine, metalloproteinase, and cartilage degradation marker concentrations in synovial fluid-and articular cartilage degeneration, measured by T1ρ and T2 quantitative magnetic resonance imaging up to 3 years after ACLR...
March 2018: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/29332250/the-levels-of-12-cytokines-and-growth-factors-in-tears-hyperthyreosis-vs-euthyreosis
#15
Jelena Juri Mandić, Ana Kozmar, Sanja Kusačić-Kuna, Anamarija Jazbec, Krešimir Mandić, Danijela Mrazovac, Nenad Vukojević
PURPOSE: Simultaneous analyses of the contents and ratios of 12 cytokines and growth factors in single samples of human tears were performed, and the results were compared between a group of healthy subjects and a group of patients with Graves' hyperthyreosis (GH) without thyroid-associated orbitopathy (TAO). METHODS: Determinations and concentration measurements of interleukins (IL-2, IL4, IL-6, IL-8, IL-10, IL-1α, and IL-1β) interferon (IFN-γ), tumor necrosis factor (TNF-α), monocyte chemoattractant protein (MCP-1), vascular endothelial growth factor (VEGF), and epidermal growth factor (EGF) were performed with single tear samples from 21 patients with hyperthyreosis and 22 healthy subjects...
April 2018: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/29303154/a-novel-and-less-invasive-technique-to-assess-cytokine-profile-of-vitreous-in-patients-of-diabetic-macular-oedema
#16
G Srividya, M Jain, K Mahalakshmi, S Gayathri, R Raman, N Angayarkanni
PurposeA pilot study to validate the collection of vitreous reflux (VR) after intravitreal injection using Schirmers tear strips was carried out. We assessed its efficiency for proteomics studies by estimating the differential expression of 27 cytokines using multiplexed bead array in diabetic macular oedema and proliferative diabetic retinopathy. To set, validate and assess the efficacy of Schirmer tear strips for collecting VR in patients undergoing intravitreal injections for diabetic macular oedema (DME)...
April 2018: Eye
https://www.readbyqxmd.com/read/29284427/study-design-and-baseline-findings-from-the-progression-of-ocular-findings-proof-natural-history-study-of-dry-eye
#17
MULTICENTER STUDY
Peter J McDonnell, Stephen C Pflugfelder, Michael E Stern, David R Hardten, Taryn Conway, Linda Villanueva, David A Hollander
BACKGROUND: The aim of this research is to initiate a 5-year natural history study of dry eye disease (DED) using objectively assessed and patient-reported outcomes, to explore the hypothesis that DED is a progressive condition that has substantive and measurable impacts not only on the ocular surface, but on quality of life and visual functioning. Our objective for this report is to examine the baseline data. METHODS: A multicenter, prospective, controlled, observational study of Level 2 (mild-to-moderate) DED patients based on International Task Force Delphi Panel severity grading, and controls, documented baseline measures (including tear film biomarkers and quality of life)...
December 28, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/29283120/correlation-of-interleukin-33-with-th-cytokines-and-clinical-severity-of-dry-eye-disease
#18
Guoping Luo, Yan Xin, Dajun Qin, Aihua Yan, Zhiyan Zhou, Zhaoxia Liu
PURPOSE: The purpose of this study is to determine the tear and serum protein levels of interleukin-33 (IL-33) and its correlation with Th cytokines and disease severity in dry eye (DE) syndrome. METHODS: Tear and serum samples were collected from 30 healthy volunteers, 30 DE patients with non-Sjogren's syndrome DE (NSSDE) and 30 DE patients with primary SSDE. The eight most frequent symptoms of DE were scored. All patients underwent corneal and conjunctival staining, tear film breakup time (TBUT), and Schirmer I test...
January 2018: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/29279263/tear-osteopontin-level-and-its-relationship-with-local-th1-th2-th17-treg-cytokines-in-children-with-allergic-conjunctivitis
#19
A Yan, G Luo, Z Zhou, W Hang, D Qin
BACKGROUND: Allergic conjunctivitis (AC) is one of the most common allergic ocular diseases worldwide. Osteopontin (OPN), as a recently described Th2 inflammation related protein, may play a role in the pathogenesis of AC. The aim of this study was to identify the expression of OPN in children with AC. METHODS: Eighty AC children (seasonal and perennial AC) and twenty controls were enrolled in this study. Serum and tears of different time points (during and out of the pollen season) were collected and used for enzyme-linked immunosorbent assay (ELISA) of OPN and T-help cell related cytokines, respectively...
March 2018: Allergologia et Immunopathologia
https://www.readbyqxmd.com/read/29235530/tri-microspheres-prevent-key-signs-of-dry-eye-disease-in-a-murine-inflammatory-model
#20
Michelle L Ratay, Stephen C Balmert, Abhinav P Acharya, Ashlee C Greene, Thiagarajan Meyyappan, Steven R Little
Dry eye disease (DED) is a highly prevalent, ocular disorder characterized by an abnormal tear film and ocular surface. Recent experimental data has suggested that the underlying pathology of DED involves inflammation of the lacrimal functional unit (LFU), comprising the cornea, conjunctiva, lacrimal gland and interconnecting innervation. This inflammation of the LFU ultimately results in tissue deterioration and the symptoms of DED. Moreover, an increase of pathogenic lymphocyte infiltration and the secretion of pro-inflammatory cytokines are involved in the propagation of DED-associated inflammation...
December 13, 2017: Scientific Reports
keyword
keyword
83463
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"