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Cornea transplant

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https://www.readbyqxmd.com/read/28321999/factors-influencing-the-contamination-rate-of-human-organ-cultured-corneas
#1
Daniel Röck, Johanna Wude, Karl U Bartz-Schmidt, Efdal Yoeruek, Sebastian Thaler, Tobias Röck
PURPOSE: To assess the influence of donor, environment and storage factors on the contamination rate of organ-cultured corneas, to consider the microbiological species causing corneal contamination and to investigate the corresponding sensitivities. METHODS: Data from 1340 consecutive donor corneas were analysed retrospectively. Logistic regression analysis was used to assess the influence of different factors on the contamination rate of organ-cultured corneas for transplantation...
March 21, 2017: Acta Ophthalmologica
https://www.readbyqxmd.com/read/28306601/assessing-the-impact-of-diabetes-mellitus-on-donor-corneal-endothelial-cell-density
#2
Chase A Liaboe, Benjamin T Aldrich, Pamela C Carter, Jessica M Skeie, Kimberlee A Burckart, Gregory A Schmidt, Cynthia R Reed, M Bridget Zimmerman, Mark A Greiner
PURPOSE: To quantify changes in endothelial cell density (ECD) of donor corneal tissue in relation to the presence or absence of a medical history of diabetes mellitus diagnosis, treatment, and complications. METHODS: A retrospective review was performed for all corneas collected at Iowa Lions Eye Bank between January 2012 and December 2015. For purposes of analysis, donor corneas were divided into 4 groups: nondiabetic, non-insulin-dependent diabetic, insulin-dependent diabetic without medical complications due to diabetes, and insulin-dependent diabetic with medical complications due to diabetes...
March 16, 2017: Cornea
https://www.readbyqxmd.com/read/28303370/comparison-of-graft-survival-following-penetrating-keratoplasty-and-descemet-s-stripping-endothelial-keratoplasty-in-eyes-with-a-glaucoma-drainage-device
#3
Shawn M Iverson, Oriel Spierer, George C Papachristou, William J Feuer, Wei Shi, David S Greenfield, Terrence P O'Brien
PURPOSE: To compare corneal graft survival rates after penetrating keratoplasty (PK) and Descemet's stripping endothelial keratoplasty (DSEK) in patients with a glaucoma drainage device (GDD) or medically managed glaucoma. METHODS: A retrospective chart review was conducted on consecutive patients who underwent primary PK or primary DSEK. Inclusion criteria consisted of eyes with a diagnosis of glaucoma prior to corneal transplantation and a minimum of 6 months of follow-up...
March 16, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/28299010/systemic-immunomodulatory-strategies-in-high-risk-corneal-transplantation
#4
REVIEW
Tulio B Abud, Antonio Di Zazzo, Ahmad Kheirkhah, Reza Dana
The cornea is the most commonly transplanted tissue in the body. Although corneal grafts generally have high success rates, transplantation onto inflamed and vascularized host beds, or so-called high-risk corneal transplantation, has a high rate of graft rejection. The management of this high-risk corneal transplantation is challenging and involves numerous measures. One of the key measures to prevent graft rejection in these cases is the use of systemic immunosuppressive agents. In this article, we will review the systemic immunosuppressive agents most commonly used for high-risk corneal transplantation, which include corticosteroids, cysclosporine A, tacrolimus, mycophenolate mofetil, and rapamycin...
January 2017: Journal of Ophthalmic & Vision Research
https://www.readbyqxmd.com/read/28286571/we-are-not-alone-a-case-for-the-human-microbiome-in-extra-intestinal-diseases
#5
EDITORIAL
S Shivaji
BACKGROUND: "Dysbiosis" in the gut microbiome has been implicated in auto-immune diseases, in inflammatory diseases, in some cancers and mental disorders. The challenge is to unravel the cellular and molecular basis of dysbiosis so as to understand the disease manifestation. MAIN BODY: Next generation sequencing and genome enabled technologies have led to the establishment of the composition of gut microbiomes and established that "dysbiosis" is the cause of several diseases...
2017: Gut Pathogens
https://www.readbyqxmd.com/read/28273942/human-corneal-endothelial-cell-cultivation-from-old-donor-corneas-with-forced-attachment
#6
Mohit Parekh, Sajjad Ahmad, Alessandro Ruzza, Stefano Ferrari
Human corneal endothelial cells (HCEnCs) are responsible for maintaining the transparency of the cornea. Damaged or diseased HCEnCs may cause blindness. Replacement of the diseased cells with a healthy donor endothelium is the only currently available treatment. Tissue-engineering can serve as an alternative to conventional donor corneal transplantation. Due to the global shortage of donor corneas, a wide interest in the development of cultured graft substitutes and artificial corneas has increased. Availability of the old donor corneas is higher especially for research...
December 2017: Scientific Reports
https://www.readbyqxmd.com/read/28263034/analysis-of-the-learning-curve-for-pre-cut-corneal-specimens-in-preparation-for-lamellar-transplantation-a-prospective-single-centre-consecutive-case-series-prepared-at-the-lions-new-south-wales-nsw-eye-bank
#7
Aifric Isabel Martin, Rajnesh Devasahayam, Christopher Hodge, Simon Cooper, Gerard L Sutton
IMPORTANCE: This study represents the first paper to establish a learning curve by a single technician. BACKGROUND: Preparation of pre-cut corneal endothelial grafts commenced at Lions New South Wales Eye Bank in December 2014. The primary objective of this study was to review the safety and reliability of the preparation method during the first year of production. DESIGN: Hospital-based, prospective case series PARTICIPANTS: 234 consecutive donor corneal lenticules METHODS: Donor lenticules were prepared by a single operator using a linear cutting microkeratome...
March 6, 2017: Clinical & Experimental Ophthalmology
https://www.readbyqxmd.com/read/28260988/human-corneal-endothelial-cells-expanded-in-vitro-are-a-powerful-resource-for-tissue-engineering
#8
REVIEW
Yongsong Liu, Hong Sun, Min Hu, Min Zhu, Sean Tighe, Shuangling Chen, Yuan Zhang, Chenwei Su, Subo Cai, Ping Guo
Human corneal endothelial cells have two major functions: barrier function mediated by proteins such as ZO-1 and pump function mediated by Na-K-ATPase which help to maintain visual function. However, human corneal endothelial cells are notorious for their limited proliferative capability in vivo and are therefore prone to corneal endothelial dysfunction that eventually may lead to blindness. At present, the only method to cure corneal endothelial dysfunction is by transplantation of a cadaver donor cornea with normal corneal endothelial cells...
2017: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/28257425/corneal-stromal-stem-cells-reduce-corneal-scarring-by-mediating-neutrophil-infiltration-after-wounding
#9
Andrew J Hertsenberg, Golnar Shojaati, Martha L Funderburgh, Mary M Mann, Yiqin Du, James L Funderburgh
Corneal scarring limits vision for millions of individuals worldwide. Corneal transplantation (keratoplasty) is the standard of care for corneal opacity; however, it bears the risk of graft rejection and infection and is not universally available. Stem cell therapy holds promise as an alternative to keratoplasty. Stem cells from human corneal stroma (CSSC) induce regeneration of transparent corneal tissue in a mouse wound-healing model. In this study we investigated the mechanism by which CSSC prevent deposition of fibrotic tissue...
2017: PloS One
https://www.readbyqxmd.com/read/28251158/molecular-and-histopathological-changes-associated-with-keratoconus
#10
REVIEW
Mariam Lotfy Khaled, Inas Helwa, Michelle Drewry, Mutsa Seremwe, Amy Estes, Yutao Liu
Keratoconus (KC) is a corneal thinning disorder that leads to loss of visual acuity through ectasia, opacity, and irregular astigmatism. It is one of the leading indicators for corneal transplantation in the Western countries. KC usually starts at puberty and progresses until the third or fourth decade; however its progression differs among patients. In the keratoconic cornea, all layers except the endothelium have been shown to have histopathological structural changes. Despite numerous studies in the last several decades, the mechanisms of KC development and progression remain unclear...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28247507/development-of-retrocorneal-membrane-following-pig-to-monkey-penetrating-keratoplasty
#11
Whayoung Lee, Alex Mammen, Deepinder K Dhaliwal, Cassandra Long, Yuko Miyagawa, David Ayares, David K C Cooper, Hidetaka Hara
Recent reports of long-term survival after wild-type (WT) pig-to-monkey corneal xenotransplantation are encouraging. We experienced the rapid development of retrocorneal membranes, a rare complication after corneal allotransplantation (although seen in infants and young children). The original specific aim of the study was to determine the factors associated with successful (young) pig corneal transplantation in monkeys. However, when it was obvious that retrocorneal membranes rapidly developed, our aims became to determine the factors involved in its development after both WT and Genetically engineered (GE ) pig corneal xenotransplantation and to investigate the characteristics of the retrocorneal membrane...
January 2017: Xenotransplantation
https://www.readbyqxmd.com/read/28239777/-limbal-epithelial-stem-cell-transplantation-current-state-and-perspectives
#12
D Meller, H Thomasen
Homeostasis of the corneal surface is maintained by epithelial stem cells localized in the limbus. Multiple intrinsic factors or external injuries can destroy the delicate microenvironment of limbal epithelial stem cells causing a state which is termed limbal stem cell deficiency (LSCD). In such cases, re-epithelialization of the cornea is drastically impeded and conjunctival epithelium starts to extend beyond the limbus and to invade the corneal surface. In partial LSCD, a superficial keratectomy combined with an amniotic membrane is advised and helpful to restore an intact, healthy ocular surface...
February 26, 2017: Der Ophthalmologe: Zeitschrift der Deutschen Ophthalmologischen Gesellschaft
https://www.readbyqxmd.com/read/28224301/surgical-outcomes-of-perforated-and-unperforated-corneal-descemetocele
#13
Evin Singar Ozdemir, Ayse Burcu, Zuleyha Yalnız Akkaya, Firdevs Ornek
OBJECTIVE: To compare the surgical outcomes of amniotic membrane transplantation (AMT) and penetrating keratoplasty (PK) in perforated and non-perforated corneal descemetocele. METHOD: In this study, we retrospectively examined 48 eyes of 48 patients operated with AMT, and 32 eyes of 32 patients operated with PK for perforated and non-perforated descemetocele. RESULTS: There were no significant differences between the two groups with regard to age, sex, laterality, follow-up duration, cause of descemetocele, and localization (p > 0...
February 21, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/28215504/a-case-of-in-vivo-iontophoresis-assisted-corneal-collagen-cross-linking-for-keratoconus-an-immunohistochemical-study
#14
Mirko Manetti, Eleonora Favuzza, Eleonora Sgambati, Rita Mencucci, Mirca Marini
The standard corneal collagen cross-linking (CXL), that includes the removal of corneal epithelium to permit adequate penetration of riboflavin in the stroma, is an established procedure to halting keratoconus progression. However, as epithelial removal may cause postoperative pain and an increased risk of corneal infection, new therapeutic approaches have been proposed. Iontophoresis is a recently developed non-invasive technique which provides the use of electrical current during CXL to enhance transepithelial penetration of riboflavin into the corneal stroma...
February 15, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28199886/construction-of-tissue-engineered-full-thickness-cornea-substitute-using-limbal-epithelial-cell-like-and-corneal-endothelial-cell-like-cells-derived-from-human-embryonic-stem-cells
#15
Canwei Zhang, Liqun Du, Peng Sun, Lin Shen, Jing Zhu, Kunpeng Pang, Xinyi Wu
The aim of this study was to construct a full-thickness artificial cornea substitute in vitro by coculturing limbal epithelial cell-like (LEC-like) cells and corneal endothelial cell-like (CEC-like) cells derived from human embryonic stem cells (hESCs) on APCM scaffold. A 400 μm thickness, 11 mm diameter APCM lamella containing Bowman's membrane was prepared as the scaffold using trephine and a special apparatus made by ourselves. LEC-like cells and CEC-like cells, derived from hESCs as our previously described, were cocultured on the scaffold using a special insert of 24-well plates that enabled seeding both sides of the scaffold...
February 6, 2017: Biomaterials
https://www.readbyqxmd.com/read/28186681/skin-derived-precursors-as-a-source-of-progenitors-for-corneal-endothelial-regeneration
#16
Emi Inagaki, Shin Hatou, Kazunari Higa, Satoru Yoshida, Shinsuke Shibata, Hideyuki Okano, Kazuo Tsubota, Shigeto Shimmura
Corneal blindness is the fourth leading cause of blindness in the world. Current treatment is allogenic corneal transplantation, which is limited by shortage of donors and immunological rejection. Skin-derived precursors (SKPs) are postnatal stem cells, which are self-renewing, multipotent precursors that can be isolated and expanded from the dermis. Facial skin may therefore be an accessible autologous source of neural crest derived cells. SKPs were isolated from facial skin of Wnt1-Cre/Floxed EGFP mouse. After inducing differentiation with medium containing retinoic acid and GSK 3-β inhibitor, SKPs formed polygonal corneal endothelial-like cells (sTECE)...
March 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28181929/corneal-endothelial-cell-integrity-in-precut-human-donor-corneas-enhanced-by-autocrine-vasoactive-intestinal-peptide
#17
Shay-Whey M Koh, Timothy Coll, Dante Gloria, Nicholas Sprehe
PURPOSE: To demonstrate that vasoactive intestinal peptide (VIP), a corneal endothelial (CE) cell autocrine factor, maintains the integrity of corneal endothelium in human donor corneoscleral explants precut for endothelial keratoplasty. METHODS: Twelve paired human donor corneoscleral explants used as control versus VIP-treated explants (10 nM, 30 minutes, 37°C) were shipped (4°C) to the Lions Eye Institute for Transplantation and Research for precutting (Moria CBM-ALTK Keratome), shipped back to the laboratory, and cultured in ciliary neurotrophic factor (CNTF, 0...
April 2017: Cornea
https://www.readbyqxmd.com/read/28146239/combined-pi3k-akt-and-smad2-activation-promotes-corneal-endothelial-cell-proliferation
#18
Alfonso L Sabater, Enrique J Andreu, María García-Guzmán, Tania López, Gloria Abizanda, Victor L Perez, Javier Moreno-Montañés, Felipe Prósper
Purpose: The purpose of this study was to develop a culture method for expansion of corneal endothelial cells (CEC) based on the combined activation of PI3K/Akt and Smad2. Methods: Morphology, proliferation, and migration of cultured rabbit and nonhuman primate CEC were examined in the presence of the PI3K/Akt activators IGF-1 and heregulin beta in combination with the Smad2 activator activin A. Phenotypic characterization of CEC was performed at the RNA and protein levels...
February 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28114695/-functional-results-and-graft-failure-after-repeat-keratoplasty
#19
S Blug, B Seitz, A Langenbucher, N Szentmáry
Background and Purpose Keratoplasty is the oldest and most successful transplantation method in man. Despite the immunological privilege of the cornea, the graft fails in up to 10 % of patients, depending on corneal disease, quality of donor tissue and patient compliance, and repeat keratoplasty may then be necessary. The aim of this study was to verify factors which affect the functional result and graft survival after repeat keratoplasty. Patients and Methods In this single centre, consecutive retrospective case series, patients were included who were treated at Department of Ophthalmology, Saarland University Medical Centre with repeat keratoplasty between January 1st 2001 and December 31st 2010...
January 23, 2017: Klinische Monatsblätter Für Augenheilkunde
https://www.readbyqxmd.com/read/28106291/ocular-effects-of-sulfur-mustard-and-therapeutic-approaches-a-review
#20
Yunes Panahi, Seyyed Mahdi Rajaee, Amirhossein Sahebkar
Sulfur mustard (SM) is a strong blistering, highly reactive, lipophilic chemical war agent that causes injury in different organs including the skin, eyes and respiratory tract. The Eyes are especially susceptible to the consequences of SM poisoning because of the aqueous and mucosal nature of conjunctiva and cornea. DNA alkylation and depletion of glutathione, are the most important mechanisms of SM action in the eye injuries. Acute clinical symptoms are including decrease in visual acuity, dryness, photophobia, blepharospasm, conjunctivitis and complaints of foreign body sensation and soreness that gradually progress to severe ocular pain...
January 20, 2017: Journal of Cellular Biochemistry
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