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https://www.readbyqxmd.com/read/29229149/transfusion-of-ethylene-carbodiimide-fixed-donor-splenocytes-prolongs-survival-of-vascularized-skin-allografts
#1
Jianke Ding, Shiqiang Liu, Dongliang Zhang, Yajuan Song, Xianjie Ma, Chenggang Yi, Baoqiang Song, Bo Xiao, Yingjun Su, Shuzhong Guo
BACKGROUND: Allograft rejection is a major obstacle to the widespread clinical application of vascularized composite allotransplantation. Recent studies revealed a noncytoreductive strategy to protect allografts by the transfusion of ethylene carbodiimide-fixed donor splenocytes (ECDI-SPs). To determine whether this approach offers advantages in protecting skin allografts, we examined the immunological protection of infusing ECDI-SPs with a 30-d administration of rapamycin on the skin allografts of mice...
January 2018: Journal of Surgical Research
https://www.readbyqxmd.com/read/29226476/differences-in-the-activity-of-endogenous-bmp-signalling-impact-on-the-ability-of-induced-pluripotent-stem-cells-to-differentiate-to-corneal-epithelial-like-cells
#2
Taty Anna Kamarudin, Sanja Bojic, Joseph Collin, Min Yu, Sameer Alharthi, Harley Buck, Alex Shortt, Lyle Armstrong, Francisco C Figueiredo, Majlinda Lako
Cornea is a clear outermost layer of the eye which enables transmission of light onto the retina. The transparent corneal epithelium is regenerated by limbal stem cells (LSCs), whose loss/dysfunction results in limbal stem cell deficiency (LSCD). Ex vivo expansion of autologous LSCs obtained from patient's healthy eye followed by transplantation onto the LSCs damaged/deficient eye, has provided a successful treatment for unilateral LSCD. This however is not applicable to patient with total bilateral LSCD, where LSCs are lost/damaged from both eyes...
December 11, 2017: Stem Cells
https://www.readbyqxmd.com/read/29224017/the-il-2-anti-il-2-complex-attenuates-cardiac-ischaemia-reperfusion-injury-through-expansion-of-regulatory-t-cells
#3
Junhui Xiao, Kunwu Yu, Ming Li, Chuanyin Xiong, Yuzhen Wei, Qiutang Zeng
BACKGROUND/AIMS: Regulatory T cells (Tregs) can suppress immunologic damage in myocardial ischaemia/reperfusion injury (MIRI), however, the isolation and ex vivo expansion of these cells for clinical application remains challenging. Here, we investigated whether the IL-2/anti-IL-2 complex (IL-2C), a mediator of Treg expansion, can attenuate MIRI in mice. METHODS: Myocardial I/R was surgically induced in male C57BL/6 mice, aged 8-10 weeks, that were randomly assigned to 1) sham group (Sham), 2) Phosphate Buffered Saline (PBS), 3) IL-2-anti-IL-2 Ab complex (IL-2C), or 4) sham group, 5) PBS, 6) IL-2C after MIRI, or 7) IL-2C, 8) IL-2C+anti-CD25 mAbs, or 9) IL-2C; 10) IL-2C+anti-TGF-β1 mAbs, 11) IL-2C+anti-IL-10 mAbs...
December 7, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29222313/developing-t-cell-therapies-for-lymphoma-without-receptor-engineering
#4
REVIEW
Melanie Grant, Catherine M Bollard
T-cell therapy has emerged from the bench for the treatment of patients with lymphoma. Responses to T-cell therapeutics are regulated by multiple factors, including the patient's immune system status and disease stage. Outside of engineering of chimeric antigen receptors and artificial T-cell receptors, T-cell therapy can be mediated by ex vivo expansion of antigen-specific T cells targeting viral and/or nonviral tumor-associated antigens. These approaches are contributing to enhanced clinical responses and overall survival...
December 8, 2017: Hematology—the Education Program of the American Society of Hematology
https://www.readbyqxmd.com/read/29222267/new-therapeutic-targets-in-transfusion-dependent-and-independent-thalassemia
#5
REVIEW
M Domenica Cappellini, Irene Motta
β-Thalassemias are characterized by reduced production of β-globin chain, resulting in α/β-chain unbalance and precipitation of α-globin-heme complexes and determining ineffective erythropoiesis. Ineffective erythropoiesis, chronic hemolytic anemia, and compensatory hematopoietic expansion are the disease hallmarks, and they are related to the severity of the chain unbalance. Several clinical forms of β-thalassemia, including the coinheritance of β-thalassemia with hemoglobin E resulting in hemoglobin E/β-thalassemia, have been described...
December 8, 2017: Hematology—the Education Program of the American Society of Hematology
https://www.readbyqxmd.com/read/29215634/expansion-of-patient-derived-circulating-tumor-cells-from-liquid-biopsies-using-a-ctc-microfluidic-culture-device
#6
Bee Luan Khoo, Gianluca Grenci, Ying Bena Lim, Soo Chin Lee, Jongyoon Han, Chwee Teck Lim
The development of personalized cancer therapy depends on a robust system to monitor the patient's individual response to anticancer treatment. Anticancer drug efficacy has been tested on circulating tumor cells (CTCs) derived from patient blood samples after ex vivo expansion into CTC clusters. Current attempts to culture these primary cancer cells focus on long-term maintenance under growth factor supplements into cell lines, which usually takes >6 months and results in a CTC expansion efficiency of <20%...
January 2018: Nature Protocols
https://www.readbyqxmd.com/read/29211118/venous-blood-derivatives-as-fbs-substitutes-for-mesenchymal-stem-cells-a-systematic-scoping-review
#7
Luiz A Chisini, Marcus C M Conde, Guillermo Grazioli, Alissa S San Martin, Rodrigo Varella de Carvalho, Jacques E Nör, Flávio F Demarco
Although the biological properties of mesenchymal stem cells (MSC) are well-characterized in vitro, MSC clinical application is still far away to be achieved, mainly due to the need of xenogeneic substances for cell expansion, such as fetal bovine serum (FBS). FBS presents risks regarding pathogens transmissions and internalization of animal's proteins, which can unleash antigenic responses in patients after MSC implantation. A wide range of venous blood derivatives (VBD) has been reported as FBS substitutes showing promising results...
November 2017: Brazilian Dental Journal
https://www.readbyqxmd.com/read/29208744/severity-of-acute-infectious-mononucleosis-correlates-with-cross-reactive-influenza-cd8-t-cell-receptor-repertoires
#8
Nuray Aslan, Levi B Watkin, Anna Gil, Rabinarayan Mishra, Fransenio G Clark, Raymond M Welsh, Dario Ghersi, Katherine Luzuriaga, Liisa K Selin
Fifty years after the discovery of Epstein-Barr virus (EBV), it remains unclear how primary infection with this virus leads to massive CD8 T-cell expansion and acute infectious mononucleosis (AIM) in young adults. AIM can vary greatly in severity, from a mild transient influenza-like illness to a prolonged severe syndrome. We questioned whether expansion of a unique HLA-A2.01-restricted, cross-reactive CD8 T-cell response between influenza virus A-M158 (IAV-M1) and EBV BMLF1280 (EBV-BM) could modulate the immune response to EBV and play a role in determining the severity of AIM in 32 college students...
December 5, 2017: MBio
https://www.readbyqxmd.com/read/29208671/cxcr4-is-a-potential-target-for-diagnostic-pet-ct-imaging-in-barrett-s-dysplasia-and-esophageal-adenocarcinoma
#9
Hsin-Yu Fang, Natasha Stephens Münch, Margret Schottelius, Jonas Ingermann, Haibo Liu, Michael Schauer, Stefan Stangl, Gabriele Multhoff, Katja Steiger, Carlos Gerngroß, Moritz Jesinghaus, Wilko Weichert, Anja A Kühl, Antonia R Sepulveda, Hans-Jürgen Wester, Timothy C Wang, Michael Quante
INTRODUCTION: Barrett's Esophagus (BE) represents an early stage in carcinogenesis leading to esophageal adenocarcinoma (EAC). Considerable evidence supports a major role for chronic inflammation and diverse chemokine pathways in the development of BE and EAC. METHODS: Here we utilized a IL-1b transgenic mouse model of BE and EAC and human patient imaging to analyse the importance of CXCR4 expressing cells during esophageal carcinogenesis. RESULTS: IL-1b overexpression induces chronic esophageal inflammation and recapitulates the progression to BE and EAC...
December 5, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/29206661/the-need-and-opportunity-for-donation-after-circulatory-death-worldwide
#10
Martí Manyalich, Helen Nelson, Francis L Delmonico
PURPOSE OF REVIEW: The global shortage of organ donors will not be resolved solely by relying on deceased donation following a brain death determination (DBD). Expansion of deceased donation after circulatory death (DCD) will be needed to address the shortfall of organs for transplantation. Approximately 120 000 organ transplants are performed each year; however, the WHO estimates that this number of transplants only resolves 10% of the annual worldwide transplant need. RECENT FINDINGS: The report addresses the opportunity of DCD expansion by evaluating the DCD potential that is not being realized, the utility of DCD enabling DBD to emerge in some clinical situations, by the effectiveness of a donor registry in achieving DCD, and by the current clinical research of heart, lung, and liver transplantation from DCD...
December 4, 2017: Current Opinion in Organ Transplantation
https://www.readbyqxmd.com/read/29198753/cell-therapies-for-hematological-malignancies-don-t-forget-non-gene-modified-t-cells
#11
REVIEW
Melanie L Grant, Catherine M Bollard
Cell therapy currently performs an important role in the treatment of patients with various hematological malignancies. The response to the cell therapy is regulated by multiple factors including the patient's immune system status, genetic profile, stage at diagnosis, age, and underlying disease. Cell therapy that does not require genetic manipulation can be mediated by donor lymphocyte infusion strategies, selective depletion in the post-transplant setting and the ex vivo expansion of antigen-specific T cells...
November 27, 2017: Blood Reviews
https://www.readbyqxmd.com/read/29196968/nk-cell-therapy-after-hematopoietic-stem-cell-transplantation-can-we-improve-anti-tumor-effect
#12
REVIEW
Catharina H M J Van Elssen, Stefan O Ciurea
After decades since the discovery of natural killer (NK) cells as potential effector cells fighting malignantly transformed and virally infected cells, little progress has been made in their clinical application. This yet unrealized therapeutic effect is presumably, at least in part, due to low numbers of functional NK cells that could be obtained from the peripheral blood relative to tumor burden. Our group hypothesized that a relatively small NK cell number to targeted malignant cells is the cause of a lack of clinical effect...
December 1, 2017: International Journal of Hematology
https://www.readbyqxmd.com/read/29196891/sodium-valproate-modulates-immune-response-by-alternative-activation-of-monocyte-derived-macrophages-in-systemic-lupus-erythematosus
#13
Saeed Mohammadi, Marie Saghaeian-Jazi, Sima Sedighi, Ali Memarian
The anti-inflammatory role of macrophages in apoptotic cells (ACs) clearance is involved in Systemic Lupus Erythematosus (SLE) pathogenesis. The efferocytic capability of macrophages is altered by M1/M2 polarization. Histone deacetylase inhibitors (HDACi) are proposed to enhance the expansion of M2 macrophages. Sodium valproate (VPA) is an HDACi with different anti-inflammatory properties. Here, we aimed to investigate the effects of HDACi by VPA on the polarization of monocyte-derived macrophages (MDMs) and regulating the expression of anti-inflammatory cytokines in SLE...
December 1, 2017: Clinical Rheumatology
https://www.readbyqxmd.com/read/29189387/ex-vivo-expanded-natural-killer-cells-derived-from-long-term-cryopreserved-cord-blood-are-cytotoxic-against-primary-breast-cancer-cells
#14
Tina Nham, Sophie M Poznanski, Isabella Y Fan, Fatemeh Vahedi, Mira M Shenouda, Amanda J Lee, Marianne V Chew, Richard T Hogg, Dean A Lee, Ali A Ashkar
With over 600,000 units of umbilical cord blood (CB) stored on a global scale, it is important to elucidate the therapeutic abilities of this cryopreserved reservoir. In the advancing field of natural killer (NK) cell cancer immunotherapy, CB has proven to be a promising and noninvasive source of therapeutic NK cells. Although studies have proven the clinical efficacy of using long-term cryopreserved CB in the context of hematopoietic stem cell transplantations, little is known about its use for the ex vivo expansion of effector immune cells...
November 17, 2017: Journal of Immunotherapy
https://www.readbyqxmd.com/read/29178199/hematopoietic-niche-exploring-biomimetic-cues-to-improve-the-functionality-of-hematopoietic-stem-progenitor-cells
#15
REVIEW
Marta H G Costa, António M de Soure, Joaquim M S Cabral, Frederico Castelo Ferreira, Cláudia L da Silva
The adult bone marrow (BM) niche is a complex entity where a homeostatic hematopoietic system is maintained through a dynamic crosstalk between different cellular and non-cellular players. Signaling mechanisms triggered by cell-cell, cell-extracellular matrix (ECM), cell-cytokine interactions and local microenvironment parameters are involved in controlling quiescence, self-renewal, differentiation and migration of hematopoietic stem/progenitor cells (HSPC). A promising strategy to more efficiently expand HSPC numbers and tune their properties ex-vivo is to mimic the hematopoietic niche through integration of adjuvant stromal cells, soluble cues and/or biomaterial-based approaches in HSPC culture systems...
November 27, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/29174976/deposition-pattern-of-aerosolized-legionella-using-an-ex-vivo-human-porcine-respiratory-model
#16
Sophie Perinel, Valérie Forest, Mathilde Landraud, Jérémie Pourchez, Françoise Girardot, Serge Riffard, Magalie Stauffert, Jean-Michel Vergnon, Séverine Allegra
Legionella are bacteria responsible for severe lung pathologies. However how they enter and are deposited within the respiratory tract remains poorly documented. Data using animal testing led to the establishment of mathematical models allowing the estimation of aerosol dispersion risks. But direct extrapolation to humans is questionable and experimental models more physiologically representative of the inhalation route are welcome. The aim of this study was to develop a model as close as possible to the human anatomy and physiology allowing determining the deposition pattern of aerosolized Legionella while limiting in vivo experiments...
November 20, 2017: International Journal of Hygiene and Environmental Health
https://www.readbyqxmd.com/read/29168461/study-of-stem-cell-homing-self-renewal-marker-gene-profile-of-ex-vivo-expanded-human-cd34-cells-manipulated-with-a-mixture-of-cytokines-stromal-cell-derived-factor-1
#17
Jyoti Kode, Navin Khattry, Ashish Bakshi, Vasanti Amrutkar, Bhausaheb Bagal, Rohini Karandikar, Pallavi Rane, Nobutaka Fujii, Shubhada Chiplunkar
BACKGROUND & OBJECTIVES: Next generation transplantation medicine aims to develop stimulating cocktail for increased ex vivo expansion of primitive hematopoietic stem and progenitor cells (HSPC). The present study was done to evaluate the cocktail GF (Thrombopoietin + Stem Cell factor + Flt3-ligand) and homing-defining molecule Stromal cell-derived factor 1 (SDF1) for HSPC ex vivo expansion. METHODS: Peripheral blood stem cell (n=74) harvests were analysed for CD34hiCD45lo HSPC...
July 2017: Indian Journal of Medical Research
https://www.readbyqxmd.com/read/29167227/pericardial-adipose-tissue-regulates-granulopoiesis-fibrosis-and-cardiac-function-after-myocardial-infarction
#18
Michael Horckmans, Mariaelvy Bianchini, Donato Santovito, Remco T A Megens, Jean-Yves Springael, Irene Negri, Michele Vacca, Marco Di Eusanio, Antonio Moschetta, Christian Weber, Johan Duchene, Sabine Steffens
Background -The pericardial adipose tissue (AT) contains a high density of lymphoid clusters. Is unknown whether these clusters play a role in post-myocardial infarction (MI) inflammatory responses and cardiac outcome. Methods -Lymphoid clusters were examined in epicardial AT of humans with or without coronary artery disease (CAD). Murine pericardial lymphoid clusters were visualized in mice subjected to coronary artery ligation. To study the relevance of pericardial clusters during inflammatory responses after MI, we surgically removed the pericardial AT, performed B cell depletion and GM-CSF blockade...
November 22, 2017: Circulation
https://www.readbyqxmd.com/read/29164490/immunotherapy-with-interferon-%C3%AE-induced-dendritic-cells-for-chronic-hcv-infection-the-results-of-pilot-clinical-trial
#19
Elena Chernykh, Olga Leplina, Ekaterina Oleynik, Marina Tikhonova, Tamara Tyrinova, Natalia Starostina, Alexandr Ostanin
The key role of T cells in hepatitis C virus (HCV) elimination and the ability of dendritic cells (DCs) to induce antiviral T cell responses suggest that DC vaccines could be a promising approach in the treatment of chronic HCV infection. The aim of our study was to evaluate, whether immunotherapy with DCs is safe and elicits anti-HCV T cell responses. Ten patients with HCV (genotype 1) were vaccinated with monocyte-derived DCs, generated in the presence of IFN-α (IFN-DCs) and pulsed with recombinant HCV Core and NS3 proteins...
November 21, 2017: Immunologic Research
https://www.readbyqxmd.com/read/29154990/exosomes-derived-from-human-umbilical-vein-endothelial-cells-promote-neural-stem-cell-expansion-while-maintain-their-stemness-in-culture
#20
Yi-Zhe Zhang, Fan Liu, Chang-Geng Song, Xiu-Li Cao, Yu-Fei Zhang, Hai-Ning Wu, Chen-Jun Guo, Yong-Qiang Li, Qi-Jun Zheng, Min-Hua Zheng, Hua Han
The neural stem cell (NSC) niche in subventricular zone (SVZ) of adult mammalian brain contains dense vascular plexus, where endothelial cells (ECs) regulate NSCs by releasing plenty of angiocrine factors. However, the role of ECs-derived exosomes, a novel type of mediators of intercellular communications, in the regulation of NSCs remains unclear. In the current study, primary NSCs isolated from embryonic mouse brains form more neurospheres when cultured in the presence of human umbilical vein endothelial cells (HUVECs)...
November 15, 2017: Biochemical and Biophysical Research Communications
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