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Limbal stem cell deficiency

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https://www.readbyqxmd.com/read/27914026/limbal-stem-cell-and-oral-mucosal-epithelial-transplantation-from-ex-vivo-cultivation-in-lscd-induced-rabbits-histology-and-immunologic-study-of-the-transplant-epithelial-sheet
#1
Napaporn Tananuvat, Kanokkan Bumroongkit, Chainarong Tocharusa, Umnat Mevatee, Aphisek Kongkaew, Somsanguan Ausayakhun
PURPOSE: To evaluate the results of cultivated limbal epithelial and oral mucosal epithelial transplantation (CLET and COMET) in limbal stem cell deficiency (LSCD)-induced rabbit model. MATERIALS AND METHODS: Six New Zealand white rabbits were divided into two groups of three rabbits each. Limbal tissue was harvested from the first group, and oral mucosal biopsy was obtained from the second group. The tissues were cultured using an explant technique with amniotic membrane as a substrate and co-culture with the 3T3 fibroblast and air-lifting method...
December 2, 2016: International Ophthalmology
https://www.readbyqxmd.com/read/27911610/successful-consecutive-expansion-of-limbal-explants-using-a-biosafe-culture-medium-under-feeder-layer-free-conditions
#2
Marina López-Paniagua, Teresa Nieto-Miguel, Ana de la Mata, Sara Galindo, José M Herreras, Rosa M Corrales, Margarita Calonge
PURPOSE: Transplantation of in vitro cultured limbal epithelial stem cells (LESCs) is a treatment widely used for LESC deficiency. However, the number of limbal tissue donors is limited, and protocols for LESC cultivation often include compounds and/or feeder layers that can induce side effects and/or increase the cost of the culture procedure. We investigated the feasibility of obtaining more than one limbal primary culture (LPC) from the same biopsy using a culture medium in which several potentially harmful compounds were replaced at the same time by biosafe supplements, allowing the LESC cultivation without feeder layers...
December 2, 2016: Current Eye Research
https://www.readbyqxmd.com/read/27911382/a-simple-mechanical-procedure-to-create-limbal-stem-cell-deficiency-in-mouse
#3
Neda Afsharkhamseh, Elham Ghahari, Medi Eslani, Ali R Djalilian
Limbal stem cell deficiency (LSCD) is a state of malfunction or loss of limbal epithelial stem cells, after which the corneal epithelium is replaced with conjunctiva. Patients suffer from recurrent corneal defects, pain, inflammation, and loss of vision. Previously, a murine model of LSCD was described and compared to two other models. The goal was to produce a consistent mouse model of LSCD that both mimics the phenotype in humans and lasts long enough to make it possible to study the disease pathophysiology and to evaluate new treatments...
November 17, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27909310/undiluted-serum-eye-drops-for-the-treatment-of-persistent-corneal-epitheilal-defects
#4
Kaevalin Lekhanont, Passara Jongkhajornpong, Thunyarat Anothaisintawee, Varintorn Chuckpaiwong
Several studies found that 50-100% serum eye drops provided greater benefits without inducing detrimental effects on the corneal epithelial healing. This study assessed the efficacy of undiluted serum eye drops for the treatment of persistent corneal epithelial defects (PED). A total of 109 eyes received 100% serum eye drops for PED were recruited into this study. The data were compared with an historical control group of 79 eyes with PED who received conventional treatments from 2006-2011 at the same institution...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27888184/amnion-assisted-conjunctival-epithelial-redirection-in-limbal-stem-cell-grafting
#5
Harminder S Dua, Ammar Miri, Mohamed S Elalfy, Anna Lencova, Dalia G Said
AIMS: To develop a technique using amniotic membrane (AM) to prevent admixture of conjunctival epithelial cells and limbal explant-derived corneal epithelial cells in patients undergoing limbal stem cell transplantation. To compare this technique with the current method of 'sequential sector conjunctival epitheliectomy' (SSCE). METHODS: 26 patients with total limbal stem cell deficiency who underwent ocular surface reconstruction with limbal stem cells transplantation were retrospectively studied...
November 25, 2016: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/27881591/vernal-keratoconjunctivitis-culmination-of-management-using-immunosuppression-surgical-and-prosthetic-therapy-over-quarter-century
#6
Shilpa Das, Anand S Pasari, Virender S Sangwan
A 22-year-old male patient presented in 1988 with active vernal keratoconjunctivitis. He was treated with topical mast cell stabilisers and corticosteroids. Chronic inflammation despite topical treatment necessitated oral immunosuppressants. Active disease came under control with this; however, the patient gradually developed limbal stem cell deficiency. He underwent bilateral pannus resection with amniotic membrane transplantation that resulted in improved ocular surface. In 2007, patient was found to have significant bilateral posterior subcapsular cataracts and underwent bilateral cataract surgery with intraocular lens implantation with good visual outcome...
November 23, 2016: BMJ Case Reports
https://www.readbyqxmd.com/read/27877075/the-limbal-epithelial-progenitors-in-the-limbal-niche-environment
#7
REVIEW
Yuan Zhang, Hong Sun, Yongsong Liu, Shuangling Chen, Subo Cai, Yingting Zhu, Ping Guo
Limbal epithelial progenitors are stem cells located in limbal palisades of vogt. In this review, we present the audience with recent evidence that limbal epithelial progenitors may be a powerful stem cell resource for the cure of human corneal stem cell deficiency. Further understanding of their mechanism may shed lights to the future successful application of stem cell therapy not only to the eye tissue, but also to the other tissues in the human body.
2016: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/27856177/diagnostic-criteria-for-limbal-stem-cell-deficiency-a-systematic-literature-review
#8
REVIEW
Lona Jawaheer, Deepa Anijeet, Kanna Ramaesh
PURPOSE: The diagnosis of limbal stem cell deficiency (LSCD) is often based on clinical manifestations with or without the use of tests to demonstrate the presence of goblet cells or of specific epithelial markers on the corneolimbal surface. This systematic review looks at the various diagnostic methods employed in the diagnosis of LSCD in published interventional studies. DESIGN: Systematic literature review METHODS: We did a systematic search on MEDLINE and PUBMED for articles published in English between January 1, 2003 and December 31, 2013...
November 14, 2016: Survey of Ophthalmology
https://www.readbyqxmd.com/read/27832940/concomitant-simple-limbal-epithelial-transplantation-after-surgical-excision-of-ocular-surface-squamous-neoplasia
#9
Swathi Kaliki, Faraz Ali Mohammad, Prerana Tahiliani, Virender S Sangwan
PURPOSE: To compare the surgical outcomes of ocular surface squamous neoplasia (OSSN) following wide excisional biopsy with and without primary simple limbal epithelial transplantation (p-SLET). DESIGN: Non-randomized clinical study with historical controls METHODS: Setting: Single-institutional study PATIENTS: 8 patients who underwent wide excisional biopsy of OSSN without p-SLET (historical controls) and 7 patients with p-SLET (cases) INTERVENTION: Wide excisional biopsy, p-SLET MAIN OUTCOME MEASURES: Limbal stem cell deficiency (LSCD) RESULTS: The tumor features between cases versus historical controls including mean number of limbal clock hours affected by OSSN (6 vs 4; p=0...
November 7, 2016: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/27777792/the-role-of-e-cadherin-in-maintaining-the-barrier-function-of-corneal-epithelium-after-treatment-with-cultured-autologous-oral-mucosa-epithelial-cell-sheet-grafts-for-limbal-stem-deficiency
#10
Fawzia Bardag-Gorce, Richard H Hoft, Andrew Wood, Joan Oliva, Hope Niihara, Andrew Makalinao, Jacquelyn Thropay, Derek Pan, Imara Meepe, Kumar Tiger, Julio Garcia, Amanda Laporte, Samuel W French, Yutaka Niihara
The role of E-cadherin in epithelial barrier function of cultured autologous oral mucosa epithelial cell sheet (CAOMECS) grafts was examined. CAOMECS were cultured on a temperature-responsive surface and grafted onto rabbit corneas with Limbal Stem Cell Deficiency (LSCD). E-cadherin levels were significantly higher in CAOMECS compared to normal and LSCD epithelium. Beta-catenin colocalized with E-cadherin in CAOMECS cell membranes while phosphorylated beta-catenin was significantly increased. ZO-1, occludin, and Cnx43 were also strongly expressed in CAOMECS...
2016: Journal of Ophthalmology
https://www.readbyqxmd.com/read/27774903/sulfur-mustard-induced-ocular-injuries-update-on-mechanisms-and-management
#11
Yunes Panahi, Danial Roshandel, Mohammad Mehdi Sadoughi, Mostafa Ghanei, Amirhossein Sahebkar
Sulfur mustard (SM; mustard gas) is a classic chemical warfare agent that has been used in several wars and is still a potential threat especially in the Middle-East region. Victims experience acute symptoms in air-exposed organs including skin, respiratory tract and the eyes. Survivors of the acute stage might develop chronic or delayed-onset complications in the exposed organs. The exact mechanism(s) of SM-induced tissue damage is still unknown, however DNA alkylation and oxidative damage are the most relevant mechanisms...
October 21, 2016: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/27760362/cellular-stiffness-as-a-novel-stemness-marker-in-the-corneal-limbus
#12
Tom Bongiorno, Jena L Chojnowski, James D Lauderdale, Todd Sulchek
Healthy eyes contain a population of limbal stem cells (LSCs) that continuously renew the corneal epithelium. However, each year, 1 million Americans are afflicted with severely reduced visual acuity caused by corneal damage or disease, including LSC deficiency (LSCD). Recent advances in corneal transplant technology promise to repair the cornea by implanting healthy LSCs to encourage regeneration; however, success is limited to transplanted tissues that contain a sufficiently high percentage of LSCs. Attempts to screen limbal tissues for suitable implants using molecular stemness markers are confounded by the poorly understood signature of the LSC phenotype...
October 18, 2016: Biophysical Journal
https://www.readbyqxmd.com/read/27742843/a-human-corneal-epithelial-cell-line-model-for-limbal-stem-cell-biology-and-limbal-immunobiology
#13
Bakiah Shaharuddin, Sajjad Ahmad, Nani Md Latar, Simi Ali, Annette Meeson
: : Limbal stem cell (LSC) deficiency is a visually debilitating condition caused by abnormal maintenance of LSCs. It is treated by transplantation of donor-derived limbal epithelial cells (LECs), the success of which depends on the presence and quality of LSCs within the transplant. Understanding the immunobiological responses of these cells within the transplants could improve cell engraftment and survival. However, human corneal rings used as a source of LSCs are not always readily available for research purposes...
October 14, 2016: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/27742136/-limbal-stem-cell-deficiency-management-a-review
#14
REVIEW
V Kocaba, O Damour, C Auxenfans, C Burillon
Limbal stem cell deficiency is predominantly caused by severe eye burns resulting in a decreased or a complete ablation of the regenerative potential of these stem cells. The inability to reconstruct the corneal epithelium further leads conjunctivalization of the gimbal-epithelial barrier. These abnormalities collectively result in the progressive opacification of the cornea responsible for blindness that is driven by chronic corneal ulceration and neovascularization. The underlying pathology of the cornea affects the homeostasis of the neighboring conjunctiva, eyelids, and tear film...
November 2016: Journal Français D'ophtalmologie
https://www.readbyqxmd.com/read/27702552/the-effect-of-culture-medium-and-carrier-on-explant-culture-of-human-limbal-epithelium-a-comparison-of-ultrastructure-keratin-profile-and-gene-expression
#15
Meeta Pathak, O K Olstad, Liv Drolsum, Morten C Moe, Natalia Smorodinova, Sarka Kalasova, Katerina Jirsova, Bjørn Nicolaissen, Agate Noer
Patients with limbal stem cell deficiency (LSCD) often experience pain and photophobia due to recurrent epithelial defects and chronic inflammation of the cornea. Successfully restoring a healthy corneal surface in these patients by transplantation of ex vivo expanded human limbal epithelial cells (LECs) may alleviate these symptoms and significantly improve their quality of life. The clinical outcome of transplantation is known to be influenced by the quality of transplanted cells. Presently, several different protocols for cultivation and transplantation of LECs are in use...
October 1, 2016: Experimental Eye Research
https://www.readbyqxmd.com/read/27701622/regeneration-of-corneal-epithelium-with-dental-pulp-stem-cells-using-a-contact-lens-delivery-system
#16
Evgeny Kushnerev, Susan G Shawcross, Shankari Sothirachagan, Fiona Carley, Arun Brahma, Julian M Yates, M Chantal Hillarby
Purpose: The corneal epithelium is sloughed off surface of the eye by the action of blinking and is continually replaced by division and maturation of the limbal stem cells (LSCs). In the case of injury or disease, LSCs can be lost or damaged to a point at which the corneal epithelial layer is no longer maintained. leading to LSC deficiencies (LSCDs). When this occurs, the opaque conjunctiva overgrows the anterior surface of the eye, leading to vision impairment or loss. Dental pulp stem cells (DPSCs) are promising candidates as autologous LSC substitutes...
October 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27693410/an-important-role-for-adenine-cholera-toxin-hydrocortisone-and-triiodothyronine-in-the-proliferation-self-renewal-and-differentiation-of-limbal-stem-cells-in%C3%A2-vitro
#17
Min Yu, Sanja Bojic, Gustavo S Figueiredo, Paul Rooney, Julian de Havilland, Anne Dickinson, Francisco C Figueiredo, Majlinda Lako
The cornea is a self-renewing tissue located at the front of the eye. Its transparency is essential for allowing light to focus onto the retina for visual perception. The continuous renewal of corneal epithelium is supported by limbal stem cells (LSCs) which are located in the border region between conjunctiva and cornea known as the limbus. Ex vivo expansion of LSCs has been successfully applied in the last two decades to treat patients with limbal stem cell deficiency (LSCD). Various methods have been used for their expansion, yet the most widely used culture media contains a number of ingredients derived from animal sources which may compromise the safety profile of human LSC transplantation...
November 2016: Experimental Eye Research
https://www.readbyqxmd.com/read/27668496/five-year-prose-treatment-for-aniridic-keratopathy
#18
Takashi Kojima, Asato Hasegawa, Tomoaki Nakamura, Naoki Isogai, Takahiro Kataoka, Kazuo Ichikawa
PURPOSE: Aniridic keratopathy is vision-threatening condition in patients with aniridia. The keratopathy occurs due to limbal stem cell deficiency. When conventional treatments fail, surgical treatments such as corneal limbal transplantation or cultivated oral mucosal epithelium transplantation are the alternatives. Here, we report our experience with the management and monitoring of the progress of a case with aniridic keratopathy treated with a scleral lens. CASE REPORT: We describe the case of a 30-year-old woman with aniridic keratopathy in both eyes...
October 2016: Optometry and Vision Science: Official Publication of the American Academy of Optometry
https://www.readbyqxmd.com/read/27665215/corneal-epithelial-stem-cells-repopulate-the-donor-area-within-1-year-from-limbus-removal-for-limbal-autograft
#19
Massimo Busin, Claudia Breda, Marina Bertolin, Cristina Bovone, Diego Ponzin, Stefano Ferrari, Vanessa Barbaro, Hossein Mostafa Elbadawy
PURPOSE: To determine whether limbal epithelial stem cells (LESCs) repopulate the site harvested for limbal autograft transplantation (LAT), the expression of LESCs markers was evaluated in bioptic specimens obtained from the donor area 12 months or more after surgery. DESIGN: Interventional case series. PARTICIPANTS: Patients who underwent LAT for unilateral acquired limbal stem cell deficiency after chemical burn. METHODS: Corneal limbal explants were obtained from 2 sites, the harvested area and the untouched control area, in the donor eyes of 6 patients who previously underwent LAT for unilateral acquired limbal stem cell deficiency after chemical burn...
September 21, 2016: Ophthalmology
https://www.readbyqxmd.com/read/27591548/correlation-between-the-existence-of-the-palisades-of-vogt-and-limbal-epithelial-thickness-in-limbal-stem-cell-deficiency
#20
Qihua Le, Yujing Yang, Sophie X Deng, Jianjiang Xu
BACKGROUND: The aims of the study were to investigate limbal epithelial thickness in subjects with limbal stem cell deficiency and to evaluate the correlation between the palisades of Vogt and limbal epithelial thickness. DESIGN: Cross-sectional observational study. PARTICIPANTS: Twenty-four subjects (39 eyes) with limbal stem cell deficiency and 20 normal controls (20 eyes). METHODS: Anterior segment optical coherence tomography and laser scanning confocal microscopy were performed to assess each quadrant of the limbus...
September 3, 2016: Clinical & Experimental Ophthalmology
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