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Limbal stem cell deficiency

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https://www.readbyqxmd.com/read/28239777/-limbal-epithelial-stem-cell-transplantation-current-state-and-perspectives
#1
D Meller, H Thomasen
Homeostasis of the corneal surface is maintained by epithelial stem cells localized in the limbus. Multiple intrinsic factors or external injuries can destroy the delicate microenvironment of limbal epithelial stem cells causing a state which is termed limbal stem cell deficiency (LSCD). In such cases, re-epithelialization of the cornea is drastically impeded and conjunctival epithelium starts to extend beyond the limbus and to invade the corneal surface. In partial LSCD, a superficial keratectomy combined with an amniotic membrane is advised and helpful to restore an intact, healthy ocular surface...
February 26, 2017: Der Ophthalmologe: Zeitschrift der Deutschen Ophthalmologischen Gesellschaft
https://www.readbyqxmd.com/read/28179383/cultivated-limbal-epithelial-transplantation-and-penetrating-keratoplasty-postchemical-injury-a-14-year-follow-up
#2
Ritu Shah, Charuta Puranik, Ashik Mohamed, Virender S Sangwan
An 11-year-old girl presented to our tertiary eye care centre with a 9-month-old history of lime injury in the left eye in 1999 with vision of counting fingers close to the face. She initially underwent superficial keratectomy with amniotic membrane graft in 1999. Subsequently, cultivated limbal epithelial transplantation was performed in 2001 which improved her vision to 20/400. Following development of pannus and symblepharon in the left eye, she underwent pannus resection and conjunctival limbal autograft in 2002 and, a month later, optical penetrating keratoplasty (PK) following which her vision improved to 20/125...
February 8, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28106291/ocular-effects-of-sulfur-mustard-and-therapeutic-approaches-a-review
#3
Yunes Panahi, Seyyed Mahdi Rajaee, Amirhossein Sahebkar
Sulfur mustard (SM) is a strong blistering, highly reactive, lipophilic chemical war agent that causes injury in different organs including the skin, eyes and respiratory tract. The Eyes are especially susceptible to the consequences of SM poisoning because of the aqueous and mucosal nature of conjunctiva and cornea. DNA alkylation and depletion of glutathione, are the most important mechanisms of SM action in the eye injuries. Acute clinical symptoms are including decrease in visual acuity, dryness, photophobia, blepharospasm, conjunctivitis and complaints of foreign body sensation and soreness that gradually progress to severe ocular pain...
January 20, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28070718/first-in-human-trial-of-an-anti-5t4-antibody-monomethylauristatin-conjugate-pf-06263507-in-patients-with-advanced-solid-tumors
#4
Geoffrey I Shapiro, Ulka N Vaishampayan, Patricia LoRusso, Jeremy Barton, Steven Hua, Steven D Reich, Ronald Shazer, Carrie T Taylor, Dawei Xuan, Hossein Borghaei
Background The antibody-drug conjugate PF-06263507 targets the cell-surface, tumor-associated antigen 5T4 and consists of a humanized IgG1 conjugated to the microtubule-disrupting agent monomethylauristatin-F by a non-cleavable maleimidocaproyl linker. In this first-in-human, dose-finding trial (NCT01891669), we evaluated safety, pharmacokinetics, and preliminary antitumor activity of PF-06263507 in pretreated patients with advanced solid tumors, unselected for 5T4 expression. starting at 0.05 mg/kg, with 25, 56, and 95% dose increments, depending on observed dose-limiting toxicities (DLTs), applying a modified continual reassessment method...
January 9, 2017: Investigational New Drugs
https://www.readbyqxmd.com/read/28062196/radiotherapeutic-and-surgical-management-of-iris-melanoma-a-review
#5
REVIEW
Marko Popovic, Iqbal Ike K Ahmed, Julia DiGiovanni, Carol L Shields
We compare outcomes following radiotherapeutic and surgical management of iris melanoma. End points included local tumor recurrence, metastases, and common complications. From an initial search that yielded 231 articles, we found 17 relevant studies with 761 eyes. The gender distribution was balanced with a mean age of 52 years. Most studies focused on either proton beam (49.4%) or plaque (31.4%) radiotherapy. Rates of local recurrence (range: 0%-8%) and metastatic development (0%-5%) were favorable following radiotherapy; however, common complications included cataract (36%-73%), glaucoma (3%-92%), and corneal conditions (0%-33%)...
January 3, 2017: Survey of Ophthalmology
https://www.readbyqxmd.com/read/28025793/allogenic-cultivated-limbal-stem-cell-transplantation-versus-cadaveric-keratolimbal-allograft-in-ocular-surface-disorder-1-year-outcome
#6
Jitendra Kumar Singh Parihar, Avinash Singh Parihar, Vaibhav Kumar Jain, Jaya Kaushik, Pramod Nath
PURPOSE: To compare the outcome of allogenic ex vivo cultivated limbal stem cell transplantation (LSCT) versus cadaveric keratolimbal allograft in patients with limbal stem cell deficiency (LSCD). METHODS: In this prospective interventional study from Army Hospital Research and Referral in New Delhi, 50 eyes of 40 patients with ocular surface disorder having LSCD underwent either cultivated LSCT (group 1: 25 eyes of 20 patients) or keratolimbal allograft transplantation (group 2: 25 eyes of 20 patients)...
December 26, 2016: International Ophthalmology
https://www.readbyqxmd.com/read/27941384/characterization-of-the-corneal-subbasal-nerve-plexus-in-limbal-stem-cell-deficiency
#7
Pichaya Chuephanich, Chantaka Supiyaphun, Carolina Aravena, Tahir Kansu Bozkurt, Fei Yu, Sophie X Deng
PURPOSE: To quantify the changes in the subbasal nerve plexus in patients with limbal stem cell deficiency (LSCD) using in vivo laser scanning confocal microscopy. METHODS: In this retrospective cross-sectional comparative study, confocal images of 51 eyes of 37 patients with LSCD collected between 2010 and 2015 by the Heidelberg Retina Tomograph III Rostock Corneal Module Confocal Microscope were analyzed. Two independent observers evaluated the scans of the central cornea...
March 2017: Cornea
https://www.readbyqxmd.com/read/27937038/boston-keratoprosthesis-for-idiopathic-limbal-stem-cell-deficiency
#8
Ashik Mohamed, Ritu Shah, Virender S Sangwan
No abstract text is available yet for this article.
December 12, 2016: Ocular Immunology and Inflammation
https://www.readbyqxmd.com/read/27914026/limbal-stem-cell-and-oral-mucosal-epithelial-transplantation-from-ex-vivo-cultivation-in-lscd-induced-rabbits-histology-and-immunologic-study-of-the-transplant-epithelial-sheet
#9
Napaporn Tananuvat, Kanokkan Bumroongkit, Chainarong Tocharusa, Umnat Mevatee, Aphisek Kongkaew, Somsanguan Ausayakhun
PURPOSE: To evaluate the results of cultivated limbal epithelial and oral mucosal epithelial transplantation (CLET and COMET) in limbal stem cell deficiency (LSCD)-induced rabbit model. MATERIALS AND METHODS: Six New Zealand white rabbits were divided into two groups of three rabbits each. Limbal tissue was harvested from the first group, and oral mucosal biopsy was obtained from the second group. The tissues were cultured using an explant technique with amniotic membrane as a substrate and co-culture with the 3T3 fibroblast and air-lifting method...
December 2, 2016: International Ophthalmology
https://www.readbyqxmd.com/read/27911610/successful-consecutive-expansion-of-limbal-explants-using-a-biosafe-culture-medium-under-feeder-layer-free-conditions
#10
Marina López-Paniagua, Teresa Nieto-Miguel, Ana de la Mata, Sara Galindo, José M Herreras, Rosa M Corrales, Margarita Calonge
PURPOSE: Transplantation of in vitro cultured limbal epithelial stem cells (LESCs) is a treatment widely used for LESC deficiency. However, the number of limbal tissue donors is limited, and protocols for LESC cultivation often include compounds and/or feeder layers that can induce side effects and/or increase the cost of the culture procedure. We investigated the feasibility of obtaining more than one limbal primary culture (LPC) from the same biopsy using a culture medium in which several potentially harmful compounds were replaced at the same time by biosafe supplements, allowing the LESC cultivation without feeder layers...
December 2, 2016: Current Eye Research
https://www.readbyqxmd.com/read/27911382/a-simple-mechanical-procedure-to-create-limbal-stem-cell-deficiency-in-mouse
#11
Neda Afsharkhamseh, Elham Ghahari, Medi Eslani, Ali R Djalilian
Limbal stem cell deficiency (LSCD) is a state of malfunction or loss of limbal epithelial stem cells, after which the corneal epithelium is replaced with conjunctiva. Patients suffer from recurrent corneal defects, pain, inflammation, and loss of vision. Previously, a murine model of LSCD was described and compared to two other models. The goal was to produce a consistent mouse model of LSCD that both mimics the phenotype in humans and lasts long enough to make it possible to study the disease pathophysiology and to evaluate new treatments...
November 17, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27909310/undiluted-serum-eye-drops-for-the-treatment-of-persistent-corneal-epitheilal-defects
#12
Kaevalin Lekhanont, Passara Jongkhajornpong, Thunyarat Anothaisintawee, Varintorn Chuckpaiwong
Several studies found that 50-100% serum eye drops provided greater benefits without inducing detrimental effects on the corneal epithelial healing. This study assessed the efficacy of undiluted serum eye drops for the treatment of persistent corneal epithelial defects (PED). A total of 109 eyes received 100% serum eye drops for PED were recruited into this study. The data were compared with an historical control group of 79 eyes with PED who received conventional treatments from 2006-2011 at the same institution...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27888184/amnion-assisted-conjunctival-epithelial-redirection-in-limbal-stem-cell-grafting
#13
Harminder S Dua, Ammar Miri, Mohamed S Elalfy, Anna Lencova, Dalia G Said
AIMS: To develop a technique using amniotic membrane (AM) to prevent admixture of conjunctival epithelial cells and limbal explant-derived corneal epithelial cells in patients undergoing limbal stem cell transplantation. To compare this technique with the current method of 'sequential sector conjunctival epitheliectomy' (SSCE). METHODS: 26 patients with total limbal stem cell deficiency who underwent ocular surface reconstruction with limbal stem cells transplantation were retrospectively studied...
November 25, 2016: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/27881591/vernal-keratoconjunctivitis-culmination-of-management-using-immunosuppression-surgical-and-prosthetic-therapy-over-quarter-century
#14
Shilpa Das, Anand S Pasari, Virender S Sangwan
A 22-year-old male patient presented in 1988 with active vernal keratoconjunctivitis. He was treated with topical mast cell stabilisers and corticosteroids. Chronic inflammation despite topical treatment necessitated oral immunosuppressants. Active disease came under control with this; however, the patient gradually developed limbal stem cell deficiency. He underwent bilateral pannus resection with amniotic membrane transplantation that resulted in improved ocular surface. In 2007, patient was found to have significant bilateral posterior subcapsular cataracts and underwent bilateral cataract surgery with intraocular lens implantation with good visual outcome...
November 23, 2016: BMJ Case Reports
https://www.readbyqxmd.com/read/27877075/the-limbal-epithelial-progenitors-in-the-limbal-niche-environment
#15
REVIEW
Yuan Zhang, Hong Sun, Yongsong Liu, Shuangling Chen, Subo Cai, Yingting Zhu, Ping Guo
Limbal epithelial progenitors are stem cells located in limbal palisades of vogt. In this review, we present the audience with recent evidence that limbal epithelial progenitors may be a powerful stem cell resource for the cure of human corneal stem cell deficiency. Further understanding of their mechanism may shed lights to the future successful application of stem cell therapy not only to the eye tissue, but also to the other tissues in the human body.
2016: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/27856177/diagnostic-criteria-for-limbal-stem-cell-deficiency-a-systematic-literature-review
#16
Lona Jawaheer, Deepa Anijeet, Kanna Ramaesh
The diagnosis of limbal stem cell deficiency (LSCD) is often based on clinical manifestations with or without the use of tests to demonstrate the presence of goblet cells or of specific epithelial markers on the corneolimbal surface. This systematic review looks at the various diagnostic methods used in the diagnosis of LSCD in published interventional studies. The design is a systematic literature review. We did a systematic search on MEDLINE and PUBMED for articles published in English between January 1, 2003, and December 31, 2013...
November 14, 2016: Survey of Ophthalmology
https://www.readbyqxmd.com/read/27832940/concomitant-simple-limbal-epithelial-transplantation-after-surgical-excision-of-ocular-surface-squamous-neoplasia
#17
Swathi Kaliki, Faraz Ali Mohammad, Prerana Tahiliani, Virender S Sangwan
PURPOSE: To compare the surgical outcomes of ocular surface squamous neoplasia (OSSN) following wide excisional biopsy with and without primary simple limbal epithelial transplantation (p-SLET). DESIGN: Nonrandomized clinical study with historical controls. METHODS: setting: Single-institutional study. PATIENTS: Eight patients who underwent wide excisional biopsy of OSSN without p-SLET (historical controls) and 7 patients with p-SLET (cases)...
February 2017: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/27777792/the-role-of-e-cadherin-in-maintaining-the-barrier-function-of-corneal-epithelium-after-treatment-with-cultured-autologous-oral-mucosa-epithelial-cell-sheet-grafts-for-limbal-stem-deficiency
#18
Fawzia Bardag-Gorce, Richard H Hoft, Andrew Wood, Joan Oliva, Hope Niihara, Andrew Makalinao, Jacquelyn Thropay, Derek Pan, Imara Meepe, Kumar Tiger, Julio Garcia, Amanda Laporte, Samuel W French, Yutaka Niihara
The role of E-cadherin in epithelial barrier function of cultured autologous oral mucosa epithelial cell sheet (CAOMECS) grafts was examined. CAOMECS were cultured on a temperature-responsive surface and grafted onto rabbit corneas with Limbal Stem Cell Deficiency (LSCD). E-cadherin levels were significantly higher in CAOMECS compared to normal and LSCD epithelium. Beta-catenin colocalized with E-cadherin in CAOMECS cell membranes while phosphorylated beta-catenin was significantly increased. ZO-1, occludin, and Cnx43 were also strongly expressed in CAOMECS...
2016: Journal of Ophthalmology
https://www.readbyqxmd.com/read/27774903/sulfur-mustard-induced-ocular-injuries-update-on-mechanisms-and-management
#19
Yunes Panahi, Danial Roshandel, Mohammad Mehdi Sadoughi, Mostafa Ghanei, Amirhossein Sahebkar
Sulfur mustard (SM; mustard gas) is a classic chemical warfare agent that has been used in several wars and is still a potential threat especially in the Middle-East region. Victims experience acute symptoms in air-exposed organs including skin, respiratory tract and the eyes. Survivors of the acute stage might develop chronic or delayed-onset complications in the exposed organs. The exact mechanism(s) of SM-induced tissue damage is still unknown, however DNA alkylation and oxidative damage are the most relevant mechanisms...
October 21, 2016: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/27760362/cellular-stiffness-as-a-novel-stemness-marker-in-the-corneal-limbus
#20
Tom Bongiorno, Jena L Chojnowski, James D Lauderdale, Todd Sulchek
Healthy eyes contain a population of limbal stem cells (LSCs) that continuously renew the corneal epithelium. However, each year, 1 million Americans are afflicted with severely reduced visual acuity caused by corneal damage or disease, including LSC deficiency (LSCD). Recent advances in corneal transplant technology promise to repair the cornea by implanting healthy LSCs to encourage regeneration; however, success is limited to transplanted tissues that contain a sufficiently high percentage of LSCs. Attempts to screen limbal tissues for suitable implants using molecular stemness markers are confounded by the poorly understood signature of the LSC phenotype...
October 18, 2016: Biophysical Journal
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