keyword
MENU ▼
Read by QxMD icon Read
search

Limbal stem cell

keyword
https://www.readbyqxmd.com/read/27911382/a-simple-mechanical-procedure-to-create-limbal-stem-cell-deficiency-in-mouse
#1
Neda Afsharkhamseh, Elham Ghahari, Medi Eslani, Ali R Djalilian
Limbal stem cell deficiency (LSCD) is a state of malfunction or loss of limbal epithelial stem cells, after which the corneal epithelium is replaced with conjunctiva. Patients suffer from recurrent corneal defects, pain, inflammation, and loss of vision. Previously, a murine model of LSCD was described and compared to two other models. The goal was to produce a consistent mouse model of LSCD that both mimics the phenotype in humans and lasts long enough to make it possible to study the disease pathophysiology and to evaluate new treatments...
November 17, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27909310/undiluted-serum-eye-drops-for-the-treatment-of-persistent-corneal-epitheilal-defects
#2
Kaevalin Lekhanont, Passara Jongkhajornpong, Thunyarat Anothaisintawee, Varintorn Chuckpaiwong
Several studies found that 50-100% serum eye drops provided greater benefits without inducing detrimental effects on the corneal epithelial healing. This study assessed the efficacy of undiluted serum eye drops for the treatment of persistent corneal epithelial defects (PED). A total of 109 eyes received 100% serum eye drops for PED were recruited into this study. The data were compared with an historical control group of 79 eyes with PED who received conventional treatments from 2006-2011 at the same institution...
December 2, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27888184/amnion-assisted-conjunctival-epithelial-redirection-in-limbal-stem-cell-grafting
#3
Harminder S Dua, Ammar Miri, Mohamed S Elalfy, Anna Lencova, Dalia G Said
AIMS: To develop a technique using amniotic membrane (AM) to prevent admixture of conjunctival epithelial cells and limbal explant-derived corneal epithelial cells in patients undergoing limbal stem cell transplantation. To compare this technique with the current method of 'sequential sector conjunctival epitheliectomy' (SSCE). METHODS: 26 patients with total limbal stem cell deficiency who underwent ocular surface reconstruction with limbal stem cells transplantation were retrospectively studied...
November 25, 2016: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/27881591/vernal-keratoconjunctivitis-culmination-of-management-using-immunosuppression-surgical-and-prosthetic-therapy-over-quarter-century
#4
Shilpa Das, Anand S Pasari, Virender S Sangwan
A 22-year-old male patient presented in 1988 with active vernal keratoconjunctivitis. He was treated with topical mast cell stabilisers and corticosteroids. Chronic inflammation despite topical treatment necessitated oral immunosuppressants. Active disease came under control with this; however, the patient gradually developed limbal stem cell deficiency. He underwent bilateral pannus resection with amniotic membrane transplantation that resulted in improved ocular surface. In 2007, patient was found to have significant bilateral posterior subcapsular cataracts and underwent bilateral cataract surgery with intraocular lens implantation with good visual outcome...
November 23, 2016: BMJ Case Reports
https://www.readbyqxmd.com/read/27877075/the-limbal-epithelial-progenitors-in-the-limbal-niche-environment
#5
REVIEW
Yuan Zhang, Hong Sun, Yongsong Liu, Shuangling Chen, Subo Cai, Yingting Zhu, Ping Guo
Limbal epithelial progenitors are stem cells located in limbal palisades of vogt. In this review, we present the audience with recent evidence that limbal epithelial progenitors may be a powerful stem cell resource for the cure of human corneal stem cell deficiency. Further understanding of their mechanism may shed lights to the future successful application of stem cell therapy not only to the eye tissue, but also to the other tissues in the human body.
2016: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/27872138/micrornas-103-107-coordinately-regulate-macropinocytosis-and-autophagy
#6
Jong Kook Park, Han Peng, Julia Katsnelson, Wending Yang, Nihal Kaplan, Ying Dong, Joshua Z Rappoport, CongCong He, Robert M Lavker
Macropinocytosis, by which cells ingest large amounts of fluid, and autophagy, the lysosome-based catabolic process, involve vesicular biogenesis (early stage) and turnover (end stage). Much is known about early-stage events; however, our understanding of how the end stages of these processes are governed is incomplete. Here we demonstrate that the microRNA-103/107(miR-103/107) family, which is preferentially expressed in the stem cell-enriched limbal epithelium, coordinately regulates aspects of both these activities...
November 21, 2016: Journal of Cell Biology
https://www.readbyqxmd.com/read/27862636/a-novel-method-for-a-high-enrichment-of-human-corneal-epithelial-stem-cells-for-genomic-analysis
#7
Jhansi Rani Kasinathan, Venkatesh Prajna Namperumalsamy, Muthukkaruppan Veerappan, Gowri Priya Chidambaranathan
Understanding the molecular mechanisms that regulate the corneal epithelial stem cells (CESCs) in maintaining corneal homeostasis remains elusive largely due to the lack of a specific marker for their isolation. This study aims to enrich CESCs from human donor limbal epithelium and to evaluate the level of enrichment based on expression of ΔNp63α, a putative CESC marker. A two-stage enrichment of CESCs was carried out. (a) The limbal basal epithelial cells were isolated by differential enzymatic treatment and five-fold enrichment was achieved from 2% of CESCs present in the total limbal epithelium...
December 2016: Microscopy Research and Technique
https://www.readbyqxmd.com/read/27856177/diagnostic-criteria-for-limbal-stem-cell-deficiency-a-systematic-literature-review
#8
REVIEW
Lona Jawaheer, Deepa Anijeet, Kanna Ramaesh
PURPOSE: The diagnosis of limbal stem cell deficiency (LSCD) is often based on clinical manifestations with or without the use of tests to demonstrate the presence of goblet cells or of specific epithelial markers on the corneolimbal surface. This systematic review looks at the various diagnostic methods employed in the diagnosis of LSCD in published interventional studies. DESIGN: Systematic literature review METHODS: We did a systematic search on MEDLINE and PUBMED for articles published in English between January 1, 2003 and December 31, 2013...
November 14, 2016: Survey of Ophthalmology
https://www.readbyqxmd.com/read/27837564/preservation-of-ocular-epithelial-limbal-stem-cells-the-new-frontier-in-regenerative-medicine
#9
Zala Lužnik, Marina Bertolin, Claudia Breda, Barbara Ferrari, Vanessa Barbaro, Petra Schollmayer, Stefano Ferrari
Significant advances have been made in the field of ocular regenerative medicine. Promising stem cell-based therapeutic strategies have been translated into the clinical practice over the last few decades. These new stem cell-based therapies offer the possibility of permanently restoring corneal epithelium in patients with severe disabling and blinding ocular surface disease. The European Union has already classified stem cell-based therapies as "medicinal products". Therefore, manipulation is strictly regulated according to the defined conditions of good manufacturing practice, with the production of stem cell therapeutics at only accredited production sites authorized by the national regulatory agencies...
2016: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/27832940/concomitant-simple-limbal-epithelial-transplantation-after-surgical-excision-of-ocular-surface-squamous-neoplasia
#10
Swathi Kaliki, Faraz Ali Mohammad, Prerana Tahiliani, Virender S Sangwan
PURPOSE: To compare the surgical outcomes of ocular surface squamous neoplasia (OSSN) following wide excisional biopsy with and without primary simple limbal epithelial transplantation (p-SLET). DESIGN: Non-randomized clinical study with historical controls METHODS: Setting: Single-institutional study PATIENTS: 8 patients who underwent wide excisional biopsy of OSSN without p-SLET (historical controls) and 7 patients with p-SLET (cases) INTERVENTION: Wide excisional biopsy, p-SLET MAIN OUTCOME MEASURES: Limbal stem cell deficiency (LSCD) RESULTS: The tumor features between cases versus historical controls including mean number of limbal clock hours affected by OSSN (6 vs 4; p=0...
November 7, 2016: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/27777792/the-role-of-e-cadherin-in-maintaining-the-barrier-function-of-corneal-epithelium-after-treatment-with-cultured-autologous-oral-mucosa-epithelial-cell-sheet-grafts-for-limbal-stem-deficiency
#11
Fawzia Bardag-Gorce, Richard H Hoft, Andrew Wood, Joan Oliva, Hope Niihara, Andrew Makalinao, Jacquelyn Thropay, Derek Pan, Imara Meepe, Kumar Tiger, Julio Garcia, Amanda Laporte, Samuel W French, Yutaka Niihara
The role of E-cadherin in epithelial barrier function of cultured autologous oral mucosa epithelial cell sheet (CAOMECS) grafts was examined. CAOMECS were cultured on a temperature-responsive surface and grafted onto rabbit corneas with Limbal Stem Cell Deficiency (LSCD). E-cadherin levels were significantly higher in CAOMECS compared to normal and LSCD epithelium. Beta-catenin colocalized with E-cadherin in CAOMECS cell membranes while phosphorylated beta-catenin was significantly increased. ZO-1, occludin, and Cnx43 were also strongly expressed in CAOMECS...
2016: Journal of Ophthalmology
https://www.readbyqxmd.com/read/27774903/sulfur-mustard-induced-ocular-injuries-update-on-mechanisms-and-management
#12
Yunes Panahi, Danial Roshandel, Mohammad Mehdi Sadoughi, Mostafa Ghanei, Amirhossein Sahebkar
Sulfur mustard (SM; mustard gas) is a classic chemical warfare agent that has been used in several wars and is still a potential threat especially in the Middle-East region. Victims experience acute symptoms in air-exposed organs including skin, respiratory tract and the eyes. Survivors of the acute stage might develop chronic or delayed-onset complications in the exposed organs. The exact mechanism(s) of SM-induced tissue damage is still unknown, however DNA alkylation and oxidative damage are the most relevant mechanisms...
October 21, 2016: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/27770270/gadolinium-induced-effects-on-mammalian-cell-motility-adherence-and-chromatin-structure
#13
Gabor Nagy, Viktoria Baksa, Alexandra Kiss, Melinda Turani, Gaspar Banfalvi
The toxicity of gadolinium is reduced by chelating agents that render this heavy metal into contrast complexes used for medical magnetic resonance imaging. However, the dissociation of gadolinium chelates is known to generate Gd(3+) ions, the cellular toxicity of which has not been tested in details. The cytotoxic effects of Gd(III) ions were evaluated by monitoring the proliferation, measuring the cellular motility and following chromatin changes in various cell lines upon Gd(3+) treatment. Measurements applied long-term scanning microscopy and a perfusion platform that replaced the medium with test solutions, bypassed physical contact with the cell culture during experiments, and provided uninterrupted high time-resolution time-lapse photomicrography for an extended period of time...
October 22, 2016: Apoptosis: An International Journal on Programmed Cell Death
https://www.readbyqxmd.com/read/27768117/long-term-results-of-allogenic-penetrating-limbo-keratoplasty-20-years-of-experience
#14
S J Lang, D Böhringer, G Geerling, T Reinhard
AimThe objective of the study was to evaluate the long-term results of allogenic penetrating limbo-keratoplasy. This method allows simultaneous transplantation of a corneal graft and limbal stem cells of the donor by means of eccentric trephination of the donor button.MethodThe data of 192 consecutive cases of allogenic penetrating limbo-keratoplasty from 1995 to 2015 were reviewed. These had been performed exclusively in eyes with complete failure of the limbal stem cells, in combination with deep corneal scarring...
October 21, 2016: Eye
https://www.readbyqxmd.com/read/27760642/-attention-to-the-impression-cytology-in-the-evaluation-of-ocular-surface-tumors
#15
B Li, Q F Liang
Impression cytology (IC) has been widely used as a method for evaluating the ocular surface and superficial cells layers in the diagnosis and follow-up after treatment of several ocular surface tumors of both epithelial and melanocytic origin. Compared with exfoliative cytology with spatula, IC is less traumatic to the patient's eye. It provides a precise location of the area being studied and allows accurate observation of the cells the way they exist in vivo. The additional advantage of IC is the preservation of limbal stem cells responsible for continuous corneal epithelium renewal...
October 11, 2016: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/27760362/cellular-stiffness-as-a-novel-stemness-marker-in-the-corneal-limbus
#16
Tom Bongiorno, Jena L Chojnowski, James D Lauderdale, Todd Sulchek
Healthy eyes contain a population of limbal stem cells (LSCs) that continuously renew the corneal epithelium. However, each year, 1 million Americans are afflicted with severely reduced visual acuity caused by corneal damage or disease, including LSC deficiency (LSCD). Recent advances in corneal transplant technology promise to repair the cornea by implanting healthy LSCs to encourage regeneration; however, success is limited to transplanted tissues that contain a sufficiently high percentage of LSCs. Attempts to screen limbal tissues for suitable implants using molecular stemness markers are confounded by the poorly understood signature of the LSC phenotype...
October 18, 2016: Biophysical Journal
https://www.readbyqxmd.com/read/27755196/limbal-stem-cell-preservation-during-proton-beam-irradiation-for-diffuse-iris-melanoma
#17
Arun D Singh, William J Dupps, Charles V Biscotti, John H Suh, Kira L Lathrop, John P Nairn, Helen Shih
PURPOSE: To report the outcome after limbal stem cell preservation during proton beam irradiation for diffuse iris melanoma. METHODS: This is a single-case report of diffuse iris melanoma that was managed with proton beam radiation (53 Gy), wherein preemptively harvested superior and inferior limbal stem cells before radiation were replaced after irradiation. Regeneration of the palisades of Vogt and the limbal stem cells was documented by an optical coherence tomography-based imaging protocol...
October 14, 2016: Cornea
https://www.readbyqxmd.com/read/27752366/swept-source-optical-coherence-tomography-angiography-for-contact-lens-related-corneal-vascularization
#18
Marcus Ang, Yijun Cai, Anna C S Tan
Purpose. To describe a novel technique of adapting a swept-source optical coherence tomography angiography (OCTA) to image corneal vascularization. Methods. In this pilot cross-sectional study, we obtained 3 × 3 mm scans, where 100,000 A-scans are acquired per second with optical axial resolution of 8 μm and lateral resolution of 20 μm. This was performed with manual "XYZ" focus without the anterior segment lens, until the focus of the corneoscleral surface was clearly seen and the vessels of interest were in focus on the corresponding red-free image...
2016: Journal of Ophthalmology
https://www.readbyqxmd.com/read/27742843/a-human-corneal-epithelial-cell-line-model-for-limbal-stem-cell-biology-and-limbal-immunobiology
#19
Bakiah Shaharuddin, Sajjad Ahmad, Nani Md Latar, Simi Ali, Annette Meeson
: : Limbal stem cell (LSC) deficiency is a visually debilitating condition caused by abnormal maintenance of LSCs. It is treated by transplantation of donor-derived limbal epithelial cells (LECs), the success of which depends on the presence and quality of LSCs within the transplant. Understanding the immunobiological responses of these cells within the transplants could improve cell engraftment and survival. However, human corneal rings used as a source of LSCs are not always readily available for research purposes...
October 14, 2016: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/27742136/-limbal-stem-cell-deficiency-management-a-review
#20
REVIEW
V Kocaba, O Damour, C Auxenfans, C Burillon
Limbal stem cell deficiency is predominantly caused by severe eye burns resulting in a decreased or a complete ablation of the regenerative potential of these stem cells. The inability to reconstruct the corneal epithelium further leads conjunctivalization of the gimbal-epithelial barrier. These abnormalities collectively result in the progressive opacification of the cornea responsible for blindness that is driven by chronic corneal ulceration and neovascularization. The underlying pathology of the cornea affects the homeostasis of the neighboring conjunctiva, eyelids, and tear film...
November 2016: Journal Français D'ophtalmologie
keyword
keyword
83458
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"