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Limbal stem cell

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https://www.readbyqxmd.com/read/29149196/easy-xeno-free-and-feeder-free-method-for-isolating-and-growing-limbal-stromal-and-epithelial-stem-cells-of-the-human-cornea
#1
Djida Ghoubay-Benallaoua, Céline de Sousa, Raphaël Martos, Gaël Latour, Marie-Claire Schanne-Klein, Elisabeth Dupin, Vincent Borderie
Epithelial and stromal stem cells are required to maintain corneal transparency. The aim of the study was to develop a new method to isolate and grow both corneal stromal (SSC) and epithelial limbal (LSC) stem cells from small human limbal biopsies under culture conditions in accordance with safety requirements mandatory for clinical use in humans. Superficial limbal explants were retrieved from human donor corneo-scleral rims. Human limbal cells were dissociated by digestion with collagenase A, either after epithelial scraping or with no scraping...
2017: PloS One
https://www.readbyqxmd.com/read/29141906/keratolimbal-allograft-for-limbal-stem-cell-deficiency-after-severe-corneal-chemical-injury-a-systematic-review
#2
Swapna S Shanbhag, Hajirah N Saeed, Eleftherios I Paschalis, James Chodosh
PURPOSE: To review the published literature on outcomes of keratolimbal allograft (KLAL) for the surgical treatment of limbal stem cell deficiency (LSCD) and corneal blindness after severe corneal chemical injury. METHODS: Literature searches were conducted in the following electronic databases: MEDLINE, EMBASE, Science Citation Index, CINAHL, LILACS and the Cochrane Library. Standard systematic review methodology was applied. The main outcome measure was the proportion of eyes with best-corrected visual acuity (BCVA) ≥20/200 at last follow-up...
November 15, 2017: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/29135712/limbal-stem-cell-preservation-during-proton-beam-irradiation-for-diffuse-iris-melanoma
#3
Paul T Finger, Wolfgang A G Sauerwein
No abstract text is available yet for this article.
November 13, 2017: Cornea
https://www.readbyqxmd.com/read/29133636/diagnosis-of-limbal-stem-cell-deficiency-based-on-corneal-epithelial-thickness-measured-on-anterior-segment-optical-coherence-tomography
#4
Amit Mehtani, Mahesh Chandra Agarwal, Sushant Sharma, Santosh Chaudhary
PURPOSE: The purpose of this study is to investigate the epithelial thickness in the cornea and limbus in limbal stem cell deficiency (LSCD) using anterior segment optical coherence tomography (AS-OCT). METHODS: This was a cross-sectional, comparative study. OCT images of 30 eyes of 19 patients with LSCD collected by AS-OCT were scanned. Corneal epithelial thickness was recorded at the central cornea and the superior, nasal, inferior, and temporal limbus. Measurment of the same region of 30 normal eyes served as control...
November 2017: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/29133179/stage-related-central-corneal-epithelial-transformation-in-congenital-aniridia-associated-keratopathy
#5
Neil Lagali, Bogumil Wowra, Dariusz Dobrowolski, Tor Paaske Utheim, Per Fagerholm, Edward Wylegala
PURPOSE: To relate central corneal epithelial phenotype to degree of keratopathy in a limbal stem cell deficient population. METHODS: 37 patients (67 eyes) with aniridia-associated keratopathy (AAK) underwent corneal examination including slit lamp biomicroscopy to determine the Grade of AAK, Cochet-Bonnet esthesiometry, and in vivo confocal microscopy (IVCM) to assess morphology of the central corneal epithelium and subepithelial region. RESULTS: AAK Grade ranged from 1 (limbal involvement only) to 4 (total conjunctivalisation), with progression from Grade 1 occurring after the age of 20...
November 10, 2017: Ocular Surface
https://www.readbyqxmd.com/read/29127331/divergent-effects-of-wnt-%C3%AE-catenin-signaling-modifiers-on-the-preservation-of-human-limbal-epithelial-progenitors-according-to-culture-condition
#6
Hyun Jung Lee, J Mario Wolosin, So-Hyang Chung
Wnt signaling plays an important role in the regulation of self-renewal in stem cells. Here we investigated the effect of CHIR99021, the primary transducer of the Wnt signaling canonical pathway, and IWP2, a wide action Wnt signal blocker, on the growth and differentiation of the limbal epithelial progenitor cells when these cells are cultured in two different, common culture approaches, outgrowth from limbal biopsy explants and isolated cell seeded in low calcium medium. Consistent with their expected effects, irrespective of the culture system, IWP2 decreased total β-catenin while CHIR99021 increased it in nuclear localization...
November 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29116027/poly-ethylene-glycol-modified-silk-fibroin-membrane-as-a-carrier-for-limbal-epithelial-stem-cell-transplantation-in-a-rabbit-lscd-model
#7
Yijian Li, Yuli Yang, Lei Yang, Yuxiao Zeng, Xiaowei Gao, Haiwei Xu
BACKGROUND: Limbal epithelial stem cells (LESCs) play important roles in corneal epithelial homeostasis and regeneration, and damage to the limbus will lead to limbal stem cell deficiency (LSCD), with conjunctivalization and even visual impairment. Cultured LESCs have been used for ocular surface reconstruction, and silk fibroin (SF) membranes have shown potential as a substrate for LESC cultivation. Both culture methods and the carriers of LESCs affect outcomes following LESC transplantation...
November 7, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29113917/the-diagnosis-of-limbal-stem-cell-deficiency
#8
REVIEW
Qihua Le, Jianjiang Xu, Sophie X Deng
Limbal stem cells (LSCs) maintain the normal homeostasis and wound healing of corneal epithelium. Limbal stem cell deficiency (LSCD) is a pathologic condition that results from the dysfunction and/or an insufficient quantity of LSCs. The diagnosis of LSCD has been made mainly based on medical history and clinical signs, which often are not specific to LSCD. Methods to stage the severity of LSCD have been lacking. With the application of newly developed ocular imaging modalities and molecular methods as diagnostic tools, standardized quantitative criteria for the staging of LSCD can be established...
November 4, 2017: Ocular Surface
https://www.readbyqxmd.com/read/29109898/limbal-stem-cell-deficiency-and-treatment-with-stem-cell-transplantation
#9
REVIEW
Özlem Barut Selver, Ayşe Yağcı, Sait Eğrilmez, Mehmet Gürdal, Melis Palamar, Türker Çavuşoğlu, Utku Ateş, Ali Veral, Çağrı Güven, Jose Mario Wolosin
The cornea is the outermost tissue of the eye and it must be transparent for the maintenance of good visual function. The superficial epithelium of the cornea, which is renewed continuously by corneal stem cells, plays a critical role in the permanence of this transparency. These stem cells are localized at the cornea-conjunctival transition zone, referred to as the limbus. When this zone is affected/destroyed, limbal stem cell deficiency ensues. Loss of limbal stem cell function allows colonization of the corneal surface by conjunctival epithelium...
October 2017: Turkish Journal of Ophthalmology
https://www.readbyqxmd.com/read/29107055/native-and-synthetic-scaffolds-for-limbal-epithelial-stem-cell-transplantation
#10
REVIEW
Kim N Nguyen, Samantha Bobba, Alexander Richardson, Mijeong Park, Stephanie L Watson, Denis Wakefield, Nick Di Girolamo
Limbal stem cell deficiency (LSCD) is a complex blinding disease of the cornea, which cannot be treated with conventional corneal transplants. Instead, a stem cell (SC) graft is required to replenish the limbal epithelial stem cell (LESC) reservoir, which is ultimately responsible for regenerating the corneal epithelium. Current therapies utilize limbal tissue biopsies that harbor LESCs as well as tissue culture expanded cells. Typically, this tissue is placed on a scaffold that supports the formation of corneal epithelial cell sheets, which are then transferred to diseased eyes...
October 26, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/29105366/limbal-stem-cells-identity-developmental-origin-and-therapeutic-potential
#11
REVIEW
Gabriel Gonzalez, Yuzuru Sasamoto, Bruce R Ksander, Markus H Frank, Natasha Y Frank
The cornea is our window to the world and our vision is critically dependent on corneal clarity and integrity. Its epithelium represents one of the most rapidly regenerating mammalian tissues, undergoing full-turnover over the course of approximately 1-2 weeks. This robust and efficient regenerative capacity is dependent on the function of stem cells residing in the limbus, a structure marking the border between the cornea and the conjunctiva. Limbal stem cells (LSC) represent a quiescent cell population with proliferative capacity residing in the basal epithelial layer of the limbus within a cellular niche...
November 3, 2017: Wiley Interdisciplinary Reviews. Developmental Biology
https://www.readbyqxmd.com/read/29104070/the-role-of-amniotic-membrane-extract-eye-drop-ameed-in-in-vivo-cultivation-of-limbal-stem-cells
#12
Alireza Baradaran-Rafii, Niloufar Shayan Asl, Marzieh Ebrahimi, Sayena Jabbehdari, Shahram Bamdad, Danial Roshandel, Medi Eslani, Maryam Momeni
BACKGROUND: Limbal stem cell transplantation (LSCT) is the definitive treatment for total limbal stem cell deficiency (LSCD). This study evaluates the anatomical and visual outcomes of a surgical technique supplemented by amniotic membrane extract eye drop (AMEED) for in vivo cultivation of limbal stem cells (LSCs). METHODS: One small limbal block (2 × 1 mm) harvested from the contralateral healthy eye was transferred to the diseased eye, which had been already covered by cryopreserved amniotic membrane (N = 20)...
November 2, 2017: Ocular Surface
https://www.readbyqxmd.com/read/29099734/combined-conjunctival-limbal-autografts-and-living-related-conjunctival-limbal-allografts-for-severe-unilateral-ocular-surface-failure
#13
Albert Y Cheung, Enrica Sarnicola, Amit Govil, Edward J Holland
PURPOSE: To describe the technique and present 2 cases of a combined conjunctival limbal autograft (CLAU) and living-related conjunctival limbal allograft (lr-CLAL) procedure for treatment of severe unilateral ocular surface failure. METHODS: Interventional case series of 2 eyes of 2 patients sustaining severe thermal/chemical injuries from firework explosions. They both underwent the combined CLAU/lr-CLAL procedure followed by penetrating keratoplasty. Systemic immunosuppression consisted of oral tacrolimus and mycophenolate mofetil...
December 2017: Cornea
https://www.readbyqxmd.com/read/29099638/type-1-boston-keratoprosthesis-for-limbal-stem-cell-deficiency-in-epidermolysis-bullosa
#14
N Geetha Sravani, Ashik Mohamed, Virender S Sangwan
No abstract text is available yet for this article.
November 3, 2017: Ocular Immunology and Inflammation
https://www.readbyqxmd.com/read/29040119/prospective-clinical-trial-of-corneal-reconstruction-with-biomaterial-free-cultured-oral-mucosal-epithelial-cell-sheets
#15
Yu Jeong Kim, Hyun Ju Lee, Jin Suk Ryu, Yun Hee Kim, Saewha Jeon, Joo Youn Oh, Ho Kyung Choung, Sang In Khwarg, Won Ryang Wee, Mee Kum Kim
PURPOSE: To investigate the efficacy and safety of transplantation with biomaterial-free cultured oral mucosal epithelial cell sheets (COMECs) for ocular reconstruction in subjects with total limbal stem cell deficiency. METHODS: A prospective clinical trial (NCT02149732) was conducted in 8 subjects with total limbal stem cell deficiency after approval from the institutional review board of Seoul National University Hospital (H-0707-043-213) and the Ministry of Food and Drug Safety of Korea...
October 13, 2017: Cornea
https://www.readbyqxmd.com/read/29032107/long-term-outcomes-of-ocular-surface-stem-cell-allograft-transplantation
#16
Asadolah Movahedan, Albert Y Cheung, Medi Eslani, Gautham Mogilishetty, Amit Govil, Edward J Holland
PURPOSE: To investigate the long-term outcomes of ocular surface stem cell allograft transplantation (OSST) in patients with total limbal stem cell deficiency (LSCD) owing to various etiologies with a follow-up ≥ 5 years. DESIGN: Retrospective interventional cohort. METHODS: Setting: Single tertiary referral hospital. STUDY POPULATION: Patients who had (1) presence of total LSCD, (2) surgical treatment with at least 1 allograft OSST procedure, and (3) minimum follow-up ≥ 5 years after OSST...
October 13, 2017: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/29020119/human-adipose-derived-stem-cells-support-the-growth-of-limbal-stem-progenitor-cells
#17
Hua Mei, Sheyla González, Martin N Nakatsu, Elfren R Baclagon, Felix V Chen, Sophie X Deng
The most efficient method to expand limbal stem cells (LSCs) in vitro for clinical transplantation is to culture single LSCs directly on growth-arrested mouse fibroblast 3T3 cells. To reduce possible xenobiotic contamination from 3T3s, primary human adipose-derived stem cells (ASCs) were examined as feeder cells to support the expansion of LSCs in vitro. To optimize the ASC-supported culture, freshly isolated limbal epithelial cells in the form of single cells (SC-ASC) or cell clusters (CC-ASC) were cultured using three different methods: LSCs seeded directly on feeder cells, a 3-dimensional (3D) culture system and a 3D culture system with fibrin (fibrin 3D)...
2017: PloS One
https://www.readbyqxmd.com/read/29017529/short-review-on-human-umbilical-cord-lining-epithelial-cells-and-their-potential-clinical-applications
#18
REVIEW
Razwa Saleh, Hasan Mahmud Reza
BACKGROUND: The human umbilical cord has been studied extensively in the past two decades. It is free of ethical dilemmas, non-tumorigenic, and less immunogenic and thus provides a significant advantage over other stem cell sources. The cord lining yields both mesenchymal and epithelial stem cells. The mesenchymal cells have been appraised at length by many researchers, which led to the current review focusing on the cord lining epithelial cells (CLECs). These cells have high proliferative capacity and their superior harvest and multiplication, using the revolutionary CellOptima(TM) technology, makes them better candidates in comparison to contemporary adult stem cells...
October 10, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28966853/in-vivo-imaging-of-the-palisades-of-vogt-and-the-limbal-crypts-with-sub-micrometer-axial-resolution-optical-coherence-tomography
#19
Kostadinka Bizheva, Bingyao Tan, Benjamin MacLellan, Zohreh Hosseinaee, Erik Mason, Denise Hileeto, Luigina Sorbara
A research-grade OCT system was used to image in-vivo and without contact with the tissue, the cellular structure and microvasculature of the healthy human corneo-scleral limbus. The OCT system provided 0.95 µm axial and 4 µm (2 µm) lateral resolution in biological tissue depending on the magnification of the imaging objective. Cross-sectional OCT images acquired tangentially from the inferior limbus showed reflective, loop-like features that correspond to the fibrous folds of the palisades of Vogt (POV)...
September 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28959505/update-on-the-management-of-high-risk-penetrating-keratoplasty
#20
Sayena Jabbehdari, Alireza Baradaran Rafii, Ghasem Yazdanpanah, Pedram Hamrah, Edward J Holland, Ali R Djalilian
PURPOSE OF REVIEW: In this article, we review the indications and latest management of high-risk penetrating keratoplasty. RECENT FINDINGS: Despite the immune-privilege status of the cornea, immune-mediated graft rejection still remains the leading cause of corneal graft failure. This is particularly a problem in the high-risk graft recipients, namely patients with previous graft failure due to rejection and those with inflamed and vascularized corneal beds. A number of strategies including both local and systemic immunosuppression are currently used to increase the success rate of high-risk corneal grafts...
March 2017: Current Ophthalmology Reports
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