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Limbal stem cell

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https://www.readbyqxmd.com/read/28440250/outcomes-of-deep-anterior-lamellar-keratoplasty-following-autologous-simple-limbal-epithelial-transplant-in-pediatric-unilateral-severe-chemical-injury
#1
Divya Singh, M Vanathi, Chanchal Gupta, Noopur Gupta, Radhika Tandon
AIMS: To evaluate outcomes of deep anterior lamellar keratoplasty (DALK) in pediatric eyes with unilateral severe chemical injury which have undergone autologous simple limbal epithelial transplant (SLET). SETTINGS AND DESIGN: The study design was a retrospective case series. MATERIALS AND METHODS: This retrospective, case series of all children <16 years of age that have undergone DALK following autologous SLET procedure in unilateral severe chemical injury evaluates the outcomes and complications in the setting of a tertiary care center in North India...
March 2017: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/28426442/amniotic-membrane-use-for-management-of-corneal-limbal-stem-cell-deficiency
#2
Alfonso L Sabater, Victor L Perez
PURPOSE OF REVIEW: The current article reviews the most recent surgical techniques for management of corneal limbal stem cell deficiency (LSCD) using amniotic membrane tissue. RECENT FINDINGS: Early successes with amniotic membrane transplantation (AMT) for the treatment of ocular surface disorders have encouraged clinicians to investigate new applications. The use of AMT as a temporary patch in emergency cases in which LSCD may develop has considerably improved the prognosis of these patients...
April 19, 2017: Current Opinion in Ophthalmology
https://www.readbyqxmd.com/read/28426441/diagnosis-of-corneal-limbal-stem-cell-deficiency
#3
Kyeong Hwan Kim, Shahzad I Mian
PURPOSE OF REVIEW: A state of limbal stem cell deficiency (LSCD) can be secondary to a number of etiologies, resulting in either a reduction in the total number of limbal stem cells or an abnormality in stem cell function. Initially, the epithelium becomes irregular and hazy; however, this condition may progress to persistent corneal epithelial defects, stromal scarring, ulceration, and even perforation. Since LSCD secondary to a variety of etiologies may be reversible, and various factors are prognostic of disease progression, timely diagnosis is important...
April 19, 2017: Current Opinion in Ophthalmology
https://www.readbyqxmd.com/read/28406800/simple-limbal-epithelial-transplantation
#4
Virender S Sangwan, John A H Sharp
PURPOSE OF REVIEW: Simple limbal epithelial transplant (SLET) is a technique for addressing limbal stem cell deficiency. Limbal tissue from a donor eye, typically the patient's fellow healthy eye, is transplanted onto an amniotic membrane attached to the surface of the diseased eye. SLET was developed to address limitations of other techniques. Specifically, the technical difficulty of ex-vivo expansion of cells required in some techniques and the larger amount of valuable limbal tissue harvested in techniques not relying on ex-vivo expansion...
April 12, 2017: Current Opinion in Ophthalmology
https://www.readbyqxmd.com/read/28402214/eyeing-autophagy-and-macropinocytosis-in-the-corneal-limbal-epithelia
#5
Han Peng, Jong Kook Park, Robert M Lavker
Macroautophagy/autophagy is vital for cellular homeostasis and helps cells respond to various stress situations. Macropinocytosis enables cells to nonselectively engulf and take up large volumes of fluid and is known to supply amino acids to cells. The stem cell-enriched limbal epithelium has the machinery necessary to carry out both autophagy and macropinocytosis; however, both processes are relatively understudied in this tissue. We have demonstrated that these processes are linked via MIR103-MIR107, a microRNA family that is limbal epithelial-preferred...
February 15, 2017: Autophagy
https://www.readbyqxmd.com/read/28399069/conjunctival-limbal-autograft
#6
Sheraz M Daya
PURPOSE OF REVIEW: To provide an overview of conjunctival-limbal autografting (CLAU) for ocular surface rehabilitation with emphasis on more recent literature detailing outcomes of the procedure over the last 2 decades as well as technique variations and adjuvant techniques. RECENT FINDINGS: Limbal autografting initially described in 1964 by Barraquer and Strampelli and later popularized by Kenyon and Tseng is considered the best option for restoration of corneal phenotype in unilateral limbal stem-cell deficiency...
April 8, 2017: Current Opinion in Ophthalmology
https://www.readbyqxmd.com/read/28399066/limbal-and-corneal-epithelial-homeostasis
#7
Ghasem Yazdanpanah, Sayena Jabbehdari, Ali R Djalilian
PURPOSE OF REVIEW: The aim of this review is to describe the underlying mechanisms of corneal epithelial homeostasis in addition to illustrating the vital role of the limbal epithelial stem cells (LESCs) and the limbal niche in epithelial regeneration and wound healing. RECENT FINDINGS: The shedded corneal epithelial cells are constantly replenished by the LESCs which give rise to epithelial cells that proliferate, differentiate, and migrate centripetally. While some recent studies have proposed that epithelial stem cells may also be present in the central cornea, the predominant location for the stem cells is the limbus...
April 8, 2017: Current Opinion in Ophthalmology
https://www.readbyqxmd.com/read/28399065/cultivated-limbal-epithelial-transplantation
#8
Paolo Rama, Giulio Ferrari, Graziella Pellegrini
PURPOSE OF REVIEW: To provide an updated literature review on the status of cultivated limbal (corneal) epithelial transplantation. Cultivated limbal stem-cell transplantation recently received regulatory approval. We provide a comprehensive overview of recent developments in the field. RECENT FINDINGS: The current article reviews and highlights recent developments in the field of cultivated limbal stem-cell transplantation as retrieved from a literature search for the last year...
April 8, 2017: Current Opinion in Ophthalmology
https://www.readbyqxmd.com/read/28386069/tissue-harvesting-site-and-culture-medium-affect-attachment-growth-and-phenotype-of-ex-vivo-expanded-oral-mucosal-epithelial-cells
#9
Rakibul Islam, Jon Roger Eidet, Reza A Badian, Marit Lippestad, Edward Messelt, May Griffith, Darlene A Dartt, Tor Paaske Utheim
Transplantation of cultured oral mucosal epithelial cells (OMECs) is a promising treatment strategy for limbal stem cell deficiency. In order to improve the culture method, we investigated the effects of four culture media and tissue harvesting sites on explant attachment, growth, and phenotype of OMECs cultured from Sprague-Dawley rats. Neither choice of media or harvesting site impacted the ability of the explants to attach to the culture well. Dulbecco's modified Eagle's medium/Ham's F12 (DMEM) and Roswell Park Memorial Institute 1640 medium (RPMI) supported the largest cellular outgrowth...
April 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28379858/keratolimbal-allograft
#10
Albert Y Cheung, Edward J Holland
PURPOSE OF REVIEW: Keratolimbal allograft (KLAL) transplants limbal tissue attached to a corneoscleral carrier from a cadaveric donor to deliver a large number of stem cells to the recipient. The present article will provide a review of KLAL focusing on the recent literature. RECENT FINDINGS: Recent findings pertain to tissue selection, immunosuppression and adverse event profiles, and postoperative complications (particularly related to immunologic rejection). SUMMARY: KLAL permits the treatment of limbal stem cell deficiency eyes when there is no available living-related or autograft tissue with minimal risk of irreversible toxicity from modern systemic immunosuppression...
April 4, 2017: Current Opinion in Ophthalmology
https://www.readbyqxmd.com/read/28378048/-new-biomaterials-and-alternative-stem-cell-sources-for-the-reconstruction-of-the-limbal-stem-cell-niche
#11
P Eberwein, T Reinhard
Reconstruction of the limbal stem cell niche in patients with limbal stem cell insufficiency remains one of the most challenging tasks in the treatment of ocular surface diseases. Ex vivo expansion of limbal stem cells still has potential for optimization despite positive reports in centers worldwide. New biomaterials as well as alternative cell sources for the reconstruction of the limbal stem cell niche have been published in recent years. The aim of this review is to provide insight into new biomaterials and cell sources which may find their way into clinical routine in the upcoming decades...
April 4, 2017: Der Ophthalmologe: Zeitschrift der Deutschen Ophthalmologischen Gesellschaft
https://www.readbyqxmd.com/read/28376025/simultaneous-keratolimbal-autograft-and-penetrating-autokeratoplasty-a-single-stage-procedure-to-restore-monocular-vision-of-a-blind-patient-with-limbal-stem-cell-deficiency
#12
Elias Jarade, Mazen Amro, Ali A Haydar, Adib Hemade
PURPOSE: To describe the technique of simultaneous keratolimbal autograft and penetrating autokeratoplasty to restore monocular vision of a blind patient with limbal stem cell deficiency (LSCD). METHODS: A patient with acquired monocular vision due to loss of the left eye owing to LSCD postblast injury presented with acute loss of vision in the right eye due to central retinal artery occlusion. Simultaneous keratolimbal autograft and penetrating autokeratoplasty were performed in attempt to restore his vision in the left eye...
April 3, 2017: Cornea
https://www.readbyqxmd.com/read/28366677/change-in-vault-during-scleral-lens-trials-assessed-with-anterior-segment-optical-coherence-tomography
#13
Varsha M Rathi, Preeji S Mandathara, Srikanth Dumpati, Virender S Sangwan
PURPOSE: To assess the change in vault during scleral lens fitting with anterior segment optical coherence tomography (AS-OCT). METHODS: This study comprised of patients who had AS-OCT performed after 1h and 4h of scleral lens wear (PROSE, prosthetic replacement of the ocular surface ecosystem, Boston Foundation for Sight, Needham Heights, MA, USA). Vault was measured on AS-OCT as the distance between the front surface of the cornea in the center and the back surface of the scleral lens...
March 30, 2017: Contact Lens & Anterior Eye: the Journal of the British Contact Lens Association
https://www.readbyqxmd.com/read/28347855/role-of-high-resolution-optical-coherence-tomography-in-diagnosing-ocular-surface-squamous-neoplasia-with-coexisting-ocular-surface-diseases
#14
Marwan Atallah, Madhura Joag, Anat Galor, Guillermo Amescua, Afshan Nanji, Jianhua Wang, Victor L Perez, Sander Dubovy, Carol L Karp
PURPOSE: Coexistence of an ocular surface disease can mask the typical features of ocular surface squamous neoplasia (OSSN). The purpose of this study was to evaluate high resolution optical coherence tomography (HR-OCT) as an adjunct in the detection and differentiation of OSSN within coexisting ocular surface pathologies. METHODS: Retrospective study of 16 patients with ocular surface disease and lesions suspicious for OSSN that were evaluated with HR-OCT. HR-OCT images of the lesions were taken to look for evidence of OSSN...
March 24, 2017: Ocular Surface
https://www.readbyqxmd.com/read/28346794/comparative-study-of-xenobiotic-free-media-for-the-cultivation-of-human-limbal-epithelial-stem-progenitor-cells
#15
Sheyla González, Luxia Chen, Sophie X Deng
The culture of human limbal epithelial stem/progenitor cells (LSCs) in the presence of animal components poses the risk of cross-species contamination in clinical applications. We quantitatively compared different xenobiotic-free culture media for the cultivation of human LSCs. LSCs were cultured from 2 × 2 mm limbal tissue explants on denuded human amniotic membrane with different xenobiotic-free culture media: CnT-Prime (CnT-PR) supplemented with 0%, 1%, 5%, and 10% human serum (HS), embryonic stem cell medium (ESCM) alone or in combination with the standard supplemented hormonal epithelium medium (SHEM, control) at a 1:1 dilution ratio, and modified SHEM (mSHEM), in which cholera toxin and dimethyl sulfoxide (DMSO) were removed, isoproterenol was added, and the epidermal growth factor concentration was reduced...
April 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28339301/cultivation-of-human-oral-mucosal-explants-on-contact-lenses
#16
Barbara Zsebik, László Ujlaky-Nagy, Gergely Losonczy, György Vereb, Lili Takács
Purpose/Aim: Autologous cultivated oral mucosal (OM) epithelial transplantation has been successfully used as corneal epithelial replacement in bilateral limbal stem cell deficiency. Recently, lotrafilcon A contact lens (CL) surface was described as a suitable carrier for cultured stem cells in corneal epithelial transplantation. Our aim was to establish explant cultures from human OM on CL carriers that are free of animal-derived materials and feeder cells. MATERIALS AND METHODS: Human cadaveric 2 mm OM explants were sutured onto CL surfaces and cultivated with fetal calf serum (FCS) or human serum (HS) supplemented culture medium without feeder cells...
March 24, 2017: Current Eye Research
https://www.readbyqxmd.com/read/28336223/paediatric-idiopathic-limbal-stem-cell-deficiency
#17
Stephen J Vincent, Graham A Lee
Acquired limbal stem cell deficiency (LSCD) describes a condition in which the corneal limbal stem cells are altered or destroyed, typically due to ocular trauma, chronic allergy or inflammation. Idiopathic LSCD is a term used to describe limbal stem cell failure in the absence of any identifiable causative factor. While several cases of adult-onset LSCD have been identified previously, this case report describes a rare presentation of bilateral asymmetric idiopathic paediatric limbal stem cell deficiency in a sixteen-year-old male with an otherwise unremarkable ocular history...
March 20, 2017: Contact Lens & Anterior Eye: the Journal of the British Contact Lens Association
https://www.readbyqxmd.com/read/28300738/preliminary-results-from-the-comparison-of-simple-limbal-epithelial-transplantation-with-conjunctival-limbal-autologous-transplantation-in-severe-unilateral-chronic-ocular-burns
#18
Ritu Arora, Pallavi Dokania, Aditi Manudhane, Jawahar Lal Goyal
PURPOSE: To compare the safety, efficacy, and clinical outcomes of simple limbal epithelial transplantation (SLET) with conjunctival-limbal autologous transplantation (CLAU) in severe unilateral ocular chemical burns. MATERIALS AND METHODS: Twenty patients of unilateral chronic ocular burns with more than 270° limbal stem cell deficiency and a healthy fellow eye were divided into two groups - ten patients of Group A underwent SLET while ten patients of Group B were operated for CLAU...
January 2017: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/28297580/a-human-corneal-epithelial-cell-line-model-for-limbal-stem-cell-biology-and-limbal-immunobiology
#19
Bakiah Shaharuddin, Sajjad Ahmad, Nani Md Latar, Simi Ali, Annette Meeson
Limbal stem cell (LSC) deficiency is a visually debilitating condition caused by abnormal maintenance of LSCs. It is treated by transplantation of donor-derived limbal epithelial cells (LECs), the success of which depends on the presence and quality of LSCs within the transplant. Understanding the immunobiological responses of these cells within the transplants could improve cell engraftment and survival. However, human corneal rings used as a source of LSCs are not always readily available for research purposes...
March 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28266180/serum-from-plasma-rich-in-growth-factors-regenerates-rabbit-corneas-by-promoting-cell-proliferation-migration-differentiation-adhesion-and-limbal-stemness
#20
Jaime Etxebarria, Sara Sanz-Lázaro, Raquel Hernáez-Moya, Vanesa Freire, Juan A Durán, María-Celia Morales, Noelia Andollo
PURPOSE: To evaluate the regenerating potential and the mechanisms through which the autologous serum derived from plasma rich in growth factors (s-PRGF) favours corneal wound healing in vitro and in vivo. METHODS: We compared the effect of various concentrations of s-PRGF versus fetal bovine serum (FBS) and control treatment in rabbit primary corneal epithelial and stromal cells and wounded rabbit corneas. Cell proliferation was measured using an enzymatic colorimetric assay...
March 7, 2017: Acta Ophthalmologica
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