keyword
MENU ▼
Read by QxMD icon Read
search

Limbal stem cell

keyword
https://www.readbyqxmd.com/read/28544131/sclerocorneal-graft-and-sequential-removal-of-melted-cornea-after-severe-corneal-burn-with-perforation
#1
Anja Viestenz, Berthold Seitz, Hans-Gert Struck, Arne Viestenz
INTRODUCTION: Corneal burn grade IV usually leads to blindness. Several different surgical techniques remain challenging owing to the extensive tissue damage. Here, we introduce a novel technique with a 15 mm corneoscleral and limbal homologous graft combined with sequential autologous corneal removal ab interno, with a vitrectomy probe to save the anterior chamber angle. In vivo anatomy with optical coherence tomography is the surgical key. METHOD: A large 15 mm sclerocorneal graft is sutured on top of the remainder of the destroyed cornea and sclera after removal of the epithelium and conjunctiva, with anterior synechiolysis if necessary, peripheral iridectomy and conjunctivoplasty...
May 22, 2017: Clinical Anatomy
https://www.readbyqxmd.com/read/28538612/scleral-contact-lenses-in-an-academic-oculoplastics-clinic-epidemiology-and-emerging-considerations
#2
Harinder S Chahal, Marcela Estrada, Christine W Sindt, Jacob A Boehme, Mark A Greiner, Jeffrey A Nerad, Keith D Carter, Richard C Allen, Erin M Shriver
PURPOSE: To describe the role and efficacy of scleral contact lenses (SCLs) in the treatment of progressive keratopathy in patients who have undergone periocular surgical procedures, to investigate the financial impact of these surgical interventions, and to demonstrate the role of oculoplastic surgery in improving scleral contact lens fit. METHODS: A retrospective medical record review was performed to identify patients who both received SCLs and were examined by the oculoplastics service at the University of Iowa between January 1990 and December 2015...
May 22, 2017: Ophthalmic Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28529022/effect-of-microgravity-on-the-mesenchymal-stem-cell-characteristics-of-limbal-fibroblasts
#3
Shu-I Pao, Ke-Hung Chien, Hsin-Ting Lin, Ming-Cheng Tai, Jiann-Torng Chen, Chang-Min Liang
BACKGROUND: Mesenchymal stem cells (MSCs) are important for regenerative medicine. Limbal fibroblasts (LFs), present in the corneal limbus, have been shown to possess MSC characteristics, and can differentiate into other cell types. The current study sought to investigate the effect of microgravity on the proliferation and differentiation of LFs, and identify culture conditions to obtain a high proportion of LFs possessing MSC characteristics. METHODS: A rotary cell culture system was used to generate microgravity...
May 18, 2017: Journal of the Chinese Medical Association: JCMA
https://www.readbyqxmd.com/read/28528957/proteasomes-in-corneal-epithelial-cells-and-cultured-autologous-oral-mucosal-epithelial-cell-sheet-caomecs-graft-used-for-the-ocular-surface-regeneration
#4
Fawzia Bardag-Gorce, Richard Hoft, Imara Meepe, Julio Garcia, Kumar Tiger, Andrew Wood, Amanda Laporte, Derek Pan, Andrew Makalinao, Robert Niihara, Joan Oliva, Arjie Florentino, Amber M Gorce, Jeremy Stark, Daileen Cortez, Samuel W French, Yutaka Niihara
PURPOSE: This study focuses on characterizing proteasomes in corneal epithelial cells (CEC) and in cultured autologous oral mucosal epithelial cell sheets (CAOMECS) used to regenerate the ocular surface. METHODS: Limbal stem cell deficiency (LSCD) was surgically induced in rabbit corneas. CAOMECS was engineered and grafted onto corneas with LSCD to regenerate the ocular surface. RESULTS: LSCD caused an increase in inflammatory cells in the ocular surface, an increase in the formation of immunoproteasomes (IPR), and a decrease in the formation of constitutive proteasome (CPR)...
May 18, 2017: Ocular Surface
https://www.readbyqxmd.com/read/28510772/prevalence-incidence-and-risk-factors-for-the-development-of-glaucoma-in-patients-with-aniridia
#5
Shantha Balekudaru, Nandhini Sankaranarayanan, Sumita Agarkar
PURPOSE: To assess the prevalence, incidence, and risk factors for the development of glaucoma in patients with aniridia. METHODS: Retrospective analysis of case records of patients diagnosed as having congenital aniridia between January 1986 and December 2011 was performed. Patients with a follow-up of more than 12 months were included. RESULTS: Ninety-one patients (180 eyes) with the diagnosis of aniridia were identified from the case records...
May 15, 2017: Journal of Pediatric Ophthalmology and Strabismus
https://www.readbyqxmd.com/read/28484851/-long-term-outcome-of-keratoprosthesis-with-biological-support
#6
K Hille
BACKGROUND: In severe Sicca syndrome and limbal stem cell deficiency, vision can be restored by an artificial optical implant into the cornea (keratoprosthesis, KPro). After multiple efforts to achieve long-lasting corneal fixation of such an optical implant, two methods have become established: KPro with biological support, such as osteo-odonto-keratoprosthesis (OOKP) made from the root of patient's own tooth or osteo-keratoprosthesis (OKP), made from cortical bone of the patient's tibia; and the Boston KPro...
May 8, 2017: Der Ophthalmologe: Zeitschrift der Deutschen Ophthalmologischen Gesellschaft
https://www.readbyqxmd.com/read/28469991/donor-transmitted-melanoma-after-limbal-stem-cell-transplantation
#7
Anas Alsara, Muhammad Rafi
Limbal Stem Cells are a unique cell line located at the corneal limbus. They are responsible for regenerating and restoring corneal epithelial layers. Limbal stem cell transplantation is a promising technique that has been used to treat several hereditary and acquired corneal diseases. Cornea tissue lack vascularity. Hence, there were no special restrictions on collecting ocular tissues from donors with a diagnosis of metastatic melanoma. We are reporting a case of a patient who developed an ocular melanoma after she had limbal stem cell transplantation from a donor with history of melanoma...
April 2017: Avicenna Journal of Medicine
https://www.readbyqxmd.com/read/28465692/safety-of-cultivated-limbal-epithelial-stem-cell-transplantation-for-human-corneal-regeneration
#8
REVIEW
J Behaegel, S NĂ­ Dhubhghaill, C Koppen, N Zakaria
Ex vivo cultivated limbal stem cell transplantation is a promising technique for the treatment of limbal stem cell deficiency. While the results of the clinical trials have been extensively reported since the introduction of the technique in 1997, little has been reported regarding the potential health risks associated with production processes and transplantation techniques. Culture procedures require the use of animal and/or human-derived products, which carry the potential of introducing toxic or infectious agents through contamination with known or unknown additives...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28440250/outcomes-of-deep-anterior-lamellar-keratoplasty-following-autologous-simple-limbal-epithelial-transplant-in-pediatric-unilateral-severe-chemical-injury
#9
Divya Singh, M Vanathi, Chanchal Gupta, Noopur Gupta, Radhika Tandon
AIMS: To evaluate outcomes of deep anterior lamellar keratoplasty (DALK) in pediatric eyes with unilateral severe chemical injury which have undergone autologous simple limbal epithelial transplant (SLET). SETTINGS AND DESIGN: The study design was a retrospective case series. MATERIALS AND METHODS: This retrospective, case series of all children <16 years of age that have undergone DALK following autologous SLET procedure in unilateral severe chemical injury evaluates the outcomes and complications in the setting of a tertiary care center in North India...
March 2017: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/28426442/amniotic-membrane-use-for-management-of-corneal-limbal-stem-cell-deficiency
#10
Alfonso L Sabater, Victor L Perez
PURPOSE OF REVIEW: The current article reviews the most recent surgical techniques for management of corneal limbal stem cell deficiency (LSCD) using amniotic membrane tissue. RECENT FINDINGS: Early successes with amniotic membrane transplantation (AMT) for the treatment of ocular surface disorders have encouraged clinicians to investigate new applications. The use of AMT as a temporary patch in emergency cases in which LSCD may develop has considerably improved the prognosis of these patients...
April 19, 2017: Current Opinion in Ophthalmology
https://www.readbyqxmd.com/read/28426441/diagnosis-of-corneal-limbal-stem-cell-deficiency
#11
Kyeong Hwan Kim, Shahzad I Mian
PURPOSE OF REVIEW: A state of limbal stem cell deficiency (LSCD) can be secondary to a number of etiologies, resulting in either a reduction in the total number of limbal stem cells or an abnormality in stem cell function. Initially, the epithelium becomes irregular and hazy; however, this condition may progress to persistent corneal epithelial defects, stromal scarring, ulceration, and even perforation. Since LSCD secondary to a variety of etiologies may be reversible, and various factors are prognostic of disease progression, timely diagnosis is important...
April 19, 2017: Current Opinion in Ophthalmology
https://www.readbyqxmd.com/read/28406800/simple-limbal-epithelial-transplantation
#12
Virender S Sangwan, John A H Sharp
PURPOSE OF REVIEW: Simple limbal epithelial transplant (SLET) is a technique for addressing limbal stem cell deficiency. Limbal tissue from a donor eye, typically the patient's fellow healthy eye, is transplanted onto an amniotic membrane attached to the surface of the diseased eye. SLET was developed to address limitations of other techniques. Specifically, the technical difficulty of ex-vivo expansion of cells required in some techniques and the larger amount of valuable limbal tissue harvested in techniques not relying on ex-vivo expansion...
April 12, 2017: Current Opinion in Ophthalmology
https://www.readbyqxmd.com/read/28402214/eyeing-autophagy-and-macropinocytosis-in-the-corneal-limbal-epithelia
#13
Han Peng, Jong Kook Park, Robert M Lavker
Macroautophagy/autophagy is vital for cellular homeostasis and helps cells respond to various stress situations. Macropinocytosis enables cells to nonselectively engulf and take up large volumes of fluid and is known to supply amino acids to cells. The stem cell-enriched limbal epithelium has the machinery necessary to carry out both autophagy and macropinocytosis; however, both processes are relatively understudied in this tissue. We have demonstrated that these processes are linked via MIR103-MIR107, a microRNA family that is limbal epithelial-preferred...
February 15, 2017: Autophagy
https://www.readbyqxmd.com/read/28399069/conjunctival-limbal-autograft
#14
Sheraz M Daya
PURPOSE OF REVIEW: To provide an overview of conjunctival-limbal autografting (CLAU) for ocular surface rehabilitation with emphasis on more recent literature detailing outcomes of the procedure over the last 2 decades as well as technique variations and adjuvant techniques. RECENT FINDINGS: Limbal autografting initially described in 1964 by Barraquer and Strampelli and later popularized by Kenyon and Tseng is considered the best option for restoration of corneal phenotype in unilateral limbal stem-cell deficiency...
April 8, 2017: Current Opinion in Ophthalmology
https://www.readbyqxmd.com/read/28399066/limbal-and-corneal-epithelial-homeostasis
#15
Ghasem Yazdanpanah, Sayena Jabbehdari, Ali R Djalilian
PURPOSE OF REVIEW: The aim of this review is to describe the underlying mechanisms of corneal epithelial homeostasis in addition to illustrating the vital role of the limbal epithelial stem cells (LESCs) and the limbal niche in epithelial regeneration and wound healing. RECENT FINDINGS: The shedded corneal epithelial cells are constantly replenished by the LESCs which give rise to epithelial cells that proliferate, differentiate, and migrate centripetally. While some recent studies have proposed that epithelial stem cells may also be present in the central cornea, the predominant location for the stem cells is the limbus...
April 8, 2017: Current Opinion in Ophthalmology
https://www.readbyqxmd.com/read/28399065/cultivated-limbal-epithelial-transplantation
#16
Paolo Rama, Giulio Ferrari, Graziella Pellegrini
PURPOSE OF REVIEW: To provide an updated literature review on the status of cultivated limbal (corneal) epithelial transplantation. Cultivated limbal stem-cell transplantation recently received regulatory approval. We provide a comprehensive overview of recent developments in the field. RECENT FINDINGS: The current article reviews and highlights recent developments in the field of cultivated limbal stem-cell transplantation as retrieved from a literature search for the last year...
April 8, 2017: Current Opinion in Ophthalmology
https://www.readbyqxmd.com/read/28386069/tissue-harvesting-site-and-culture-medium-affect-attachment-growth-and-phenotype-of-ex-vivo-expanded-oral-mucosal-epithelial-cells
#17
Rakibul Islam, Jon Roger Eidet, Reza A Badian, Marit Lippestad, Edward Messelt, May Griffith, Darlene A Dartt, Tor Paaske Utheim
Transplantation of cultured oral mucosal epithelial cells (OMECs) is a promising treatment strategy for limbal stem cell deficiency. In order to improve the culture method, we investigated the effects of four culture media and tissue harvesting sites on explant attachment, growth, and phenotype of OMECs cultured from Sprague-Dawley rats. Neither choice of media or harvesting site impacted the ability of the explants to attach to the culture well. Dulbecco's modified Eagle's medium/Ham's F12 (DMEM) and Roswell Park Memorial Institute 1640 medium (RPMI) supported the largest cellular outgrowth...
April 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28379858/keratolimbal-allograft
#18
Albert Y Cheung, Edward J Holland
PURPOSE OF REVIEW: Keratolimbal allograft (KLAL) transplants limbal tissue attached to a corneoscleral carrier from a cadaveric donor to deliver a large number of stem cells to the recipient. The present article will provide a review of KLAL focusing on the recent literature. RECENT FINDINGS: Recent findings pertain to tissue selection, immunosuppression and adverse event profiles, and postoperative complications (particularly related to immunologic rejection). SUMMARY: KLAL permits the treatment of limbal stem cell deficiency eyes when there is no available living-related or autograft tissue with minimal risk of irreversible toxicity from modern systemic immunosuppression...
April 4, 2017: Current Opinion in Ophthalmology
https://www.readbyqxmd.com/read/28378048/-new-biomaterials-and-alternative-stem-cell-sources-for-the-reconstruction-of-the-limbal-stem-cell-niche
#19
P Eberwein, T Reinhard
Reconstruction of the limbal stem cell niche in patients with limbal stem cell insufficiency remains one of the most challenging tasks in the treatment of ocular surface diseases. Ex vivo expansion of limbal stem cells still has potential for optimization despite positive reports in centers worldwide. New biomaterials as well as alternative cell sources for the reconstruction of the limbal stem cell niche have been published in recent years. The aim of this review is to provide insight into new biomaterials and cell sources which may find their way into clinical routine in the upcoming decades...
April 4, 2017: Der Ophthalmologe: Zeitschrift der Deutschen Ophthalmologischen Gesellschaft
https://www.readbyqxmd.com/read/28376025/simultaneous-keratolimbal-autograft-and-penetrating-autokeratoplasty-a-single-stage-procedure-to-restore-monocular-vision-of-a-blind-patient-with-limbal-stem-cell-deficiency
#20
Elias Jarade, Mazen Amro, Ali A Haydar, Adib Hemade
PURPOSE: To describe the technique of simultaneous keratolimbal autograft and penetrating autokeratoplasty to restore monocular vision of a blind patient with limbal stem cell deficiency (LSCD). METHODS: A patient with acquired monocular vision due to loss of the left eye owing to LSCD postblast injury presented with acute loss of vision in the right eye due to central retinal artery occlusion. Simultaneous keratolimbal autograft and penetrating autokeratoplasty were performed in attempt to restore his vision in the left eye...
June 2017: Cornea
keyword
keyword
83458
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"