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Limbal stem cell

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https://www.readbyqxmd.com/read/29777801/computer-simulation-of-neutral-drift-among-limbal-epithelial-stem-cells-of-mosaic-mice
#1
John D West, Richard L Mort, Robert E Hill, Steven D Morley, J Martin Collinson
The use of mice that are mosaic for reporter gene expression underlies many lineage-tracing studies in stem cell biology. For example, using mosaic LacZ reporter mice, it was shown that limbal epithelial stem cells (LESCs) around the periphery of the cornea maintain radial sectors of the corneal epithelium and that radial stripe numbers declined with age. Originally, the corneal results were interpreted as progressive, age-related loss or irreversible inactivation of some LESC clones. In this study we used computer simulations to show that these results could also be explained by stochastic replacement of LESCs by neighbouring LESCs, leading to neutral drift of LESC populations...
May 13, 2018: Stem Cell Research
https://www.readbyqxmd.com/read/29777047/in-vivo-confocal-microscopy-indicates-an-inverse-relationship-between-the-sub-basal-corneal-plexus-and-the-conjunctivalisation-in-patients-with-limbal-stem-cell-deficiency
#2
Manuel Caro-Magdaleno, Asunción Alfaro-Juárez, Jesús Montero-Iruzubieta, Ana Fernández-Palacín, Ana Muñoz-Morales, Manuel Alberto Castilla-Martino, Consuelo Spínola-Muñoz, Enrique Rodríguez-de-la-Rúa
BACKGROUND/AIMS: Limbal stem cell deficiency (LSCD) is characterised by a marked decrease in limbal stem cells. It is classified primarily using subjective slit-lamp observations. In vivo confocal microscopy (IVCM) can non-invasively provide objective information on the condition of the limbal niche, the corneal epithelial basal cell density and the corneal sub-basal nerve plexus density (SND). We here used IVCM to evaluate changes in SND to improve LSCD classification. METHODS: We evaluated and classified 38 patients (76 eyes, 44 with LSC and 32 control eyes) using the Rama, López-García and Deng (clinical and confocal) classifications and evaluated the concordance of the confocal and clinical classifications...
May 18, 2018: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/29750121/-in-vivo-confocal-microscopic-observations-of-vortex-keratopathy-in-patients-with-amiodarone-induced-keratopathy-and-fabry-disease
#3
Yasuhito Ikegawa, Atsushi Shiraishi, Yasuhito Hayashi, Akiyoshi Ogimoto, Yuichi Ohashi
Purpose: To compare the morphology of two types of vortex keratopathy: amiodarone-induced keratopathy and the Fabry disease-associated keratopathy. Patients and Methods: Eight patients who were receiving oral amiodarone therapy and 3 patients with Fabry disease, a mother and her 2 daughters, were examined by slit-lamp biomicroscopy and in vivo confocal microscopy (IVCM) regularly. Results: Amiodarone-induced keratopathy developed in 7 of the 8 patients, and it was detected as early as 7 days by IVCM and 14 days by slit-lamp biomicroscopy...
2018: Journal of Ophthalmology
https://www.readbyqxmd.com/read/29707747/comparison-of-human-denuded-amniotic-membrane-and-porcine-small-intestine-submucosa-as-scaffolds-for-limbal-mesenchymal-stem-cells
#4
Liliana I Sous Naasani, Cristiano Rodrigues, Jéssica Gonçalves Azevedo, Aline F Damo Souza, Silvio Buchner, Márcia R Wink
Blinding corneal scarring is usually treated with allogeneic graft tissue. Nevertheless, the global shortage of donors leaves millions of patients in need of therapy. Traditional tissue engineering strategies involves the combination of cells, growth factors, and scaffolds that can supply cellular biological components allowing to restore the tissue function. The mesenchymal stem cells found in the limbal stroma (L-MSCs) have a self-renewal potential for multilineage differentiation. Thus, in this work we compared the potential of human amniotic membrane (hAM) and porcine small intestine submucosa (SIS) as scaffolds for L-MSCs, aiming at potential applications in corneal regeneration...
April 29, 2018: Stem Cell Reviews
https://www.readbyqxmd.com/read/29700361/human-limbal-niche-cells-are-a-powerful-regenerative-source-for-the-prevention-of-limbal-stem-cell-deficiency-in-a-rabbit-model
#5
Guigang Li, Yuan Zhang, Subo Cai, Ming Sun, Juan Wang, Shen Li, Xinyu Li, Sean Tighe, Shuangling Chen, Huatao Xie, Yingting Zhu
In this article, human limbal niche cells (LNC) or bone marrow derived mesenchymal stem cells (BMMSC) were used to prevent limbal stem cell deficiency (LSCD) in an alkali burn rabbit model and their results were compared. The epithelial cell defect area, corneal neovascularization, and the print cell cytometry were quantified to grade the severity of LSCD. Three months after the alkali burn, a partial LSCD was observed in the control group (no treatment) indicated by chronic corneal epithelial defects, positive corneal fluorescein staining, neovascularization and goblet cell migration...
April 26, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29684677/human-stem-cell-based-corneal-tissue-mimicking-structures-using-laser-assisted-3d-bioprinting-and-functional-bioinks
#6
Anni Sorkio, Lothar Koch, Laura Koivusalo, Andrea Deiwick, Susanna Miettinen, Boris Chichkov, Heli Skottman
There is a high demand for developing methods to produce more native-like 3D corneal structures. In the present study, we produced 3D cornea-mimicking tissues using human stem cells and laser-assisted bioprinting (LaBP). Human embryonic stem cell derived limbal epithelial stem cells (hESC-LESC) were used as a cell source for printing epithelium-mimicking structures, whereas human adipose tissue derived stem cells (hASCs) were used for constructing layered stroma-mimicking structures. The development and optimization of functional bioinks was a crucial step towards successful bioprinting of 3D corneal structures...
April 16, 2018: Biomaterials
https://www.readbyqxmd.com/read/29679460/limbal-niche-cells-are-a-potent-resource-of-adult-mesenchymal-progenitors
#7
REVIEW
Ping Guo, Hong Sun, Yuan Zhang, Sean Tighe, Shuangling Chen, Chen-Wei Su, Yongsong Liu, Hongxia Zhao, Min Hu, Yingting Zhu
Limbal niche cells located in the limbal Palisades of Vogt are mesenchymal stem cells that reside next to limbal basal epithelial cells. Limbal niche cells are progenitors that express embryonic stem cell markers such as Nanog, Nestin, Oct4, Rex1, Sox2 and SSEA4, mesenchymal cell markers such as CD73, CD90 and CD105, and angiogenesis markers such as Flk-1, CD31, CD34, VWF, PDGFRβ and α-SMA, but negative for CD45. In addition, the stemness of limbal niche cells can be maintained during their cell culture in a three-dimension environment...
April 20, 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29678720/a-completely-human-derived-biomaterial-mimicking-limbal-niche-platelet-rich-fibrin-gel
#8
Gamze Dereli Can, Özge Ekin Akdere, Mehmet Erol Can, Bahri Aydın, Nurullah Cagil, Menemşe Gümüşderelioğlu
Platelet-rich fibrin (PRF) is a natural biomaterial and has excellent biochemical and physical properties with a history of proven biocompatibility in the field of tissue engineering and regenerative medicine. Recent reports of fibrin-based matrices have offered new opportunities to apply PRF as a supplement for in vitro cell culture. Here, custom-modified human-derived PRF (HPRF) was produced via different centrifugation protocols, then, characterized by morphologically and chemically and utilized as a substrate and as a conditioned medium for limbal explant culture for the first time...
April 17, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29648915/response-to-modabber-and-harissi-dagher-s-letter-type-1-boston-keratoprosthesis-for-limbal-stem-cell-deficiency-in-epidermolysis-bullosa
#9
N Geetha Sravani, Ashik Mohamed, Virender S Sangwan
No abstract text is available yet for this article.
April 12, 2018: Ocular Immunology and Inflammation
https://www.readbyqxmd.com/read/29625489/visualizing-the-fate-of-transplanted-k14-confetti-corneal-epithelia-in-a-mouse-model-of-limbal-stem-cell-deficiency
#10
Alexander Richardson, Mijeong Park, Stephanie L Watson, Denis Wakefield, Nick Di Girolamo
Purpose: Therapies for limbal stem cell deficiency (LSCD) include stem cell (SC) grafts that regenerate the damaged ocular surface. However, the fate of transplanted cells is ill-defined. We addressed this limitation using primary corneal epithelial cells from K14-Confetti mice. Methods: Cultures of primary corneo-limbal epithelia were generated from K14-Confetti (n = 6) and wild-type (WT) (n = 3) mice. Cell phenotype and function was ascertained by immunofluorescence, flow cytometry, quantitative PCR and colony formation...
March 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29624196/highly-concentrated-collagen-solutions-leading-to-transparent-scaffolds-of-controlled-three-dimensional-organizations-for-corneal-epithelial-cell-colonization
#11
Aurélien Tidu, Djida Ghoubay-Benallaoua, Claire Teulon, Sophie Asnacios, Kate Grieve, François Portier, Marie-Claire Schanne-Klein, Vincent Borderie, Gervaise Mosser
This study aimed at controlling both the organization and the transparency of dense collagen scaffolds making use of the lyotropic mesogen properties of collagen. Cholesteric or plywood-like liquid crystal phases were achieved using mixtures of acetic and hydrochloric acids as solvents. The critical pH at which the switch between the two phases occurred was around pH = 3. The use of the two acids led to fibrillated collagen I scaffolds, whose visual aspect ranged from opaque to transparent. Rheological investigations showed that viscoelastic properties of the plywood-like solutions were optimized for molding due to faster recovery...
April 6, 2018: Biomaterials Science
https://www.readbyqxmd.com/read/29621511/spectral-domain-optical-coherence-tomography-in-limbal-stem-cell-deficiency-a-case-control-study
#12
Nilly Banayan, Cristina Georgeon, Kate Grieve, Vincent M Borderie
PURPOSE: To evaluate the efficacy of optical coherence tomography as a non-contact method for imaging the ocular surface in limbal stem cell deficiency (LSCD) and normal eyes. DESIGN: Retrospective case-control study. METHODS: SETTING: Institutional. STUDY POPULATION: 22 eyes with LSCD (study group, 22 patients) and 10 normal eyes (control group, 10 patients). OBSERVATION PROCEDURES: SD-OCT and confocal microscopy in both the limbal and central corneal zones...
April 2, 2018: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/29613762/synthetic-nanofiber-reinforced-amniotic-membrane-via-interfacial-bonding
#13
Huanhuan Liu, Zhengbing Zhou, Hui Lin, Juan Wu, Brian Ginn, Ji Suk Choi, Xuesong Jiang, Liam Chung, Jennifer H Elisseeff, Samuel Yiu, Hai-Quan Mao
Severe damage to the ocular surface can result in limbal stem cell (LSC) deficiency, which contributes to loss of corneal clarity, potential vision loss, chronic pain, photophobia, and keratoplasty failure. Human amniotic membrane (AM) is the most effective substrate for LSC transplantation to treat patients with LSC deficiency. However, the widespread use of the AM in the clinic remains a challenge because of the high cost for preserving freshly prepared AM and the weak mechanical strength of lyophilized AM...
April 19, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29597010/boston-keratoprosthesis-type-1-for-limbal-stem-cell-deficiency-after-severe-chemical-corneal-injury-a-systematic-review
#14
REVIEW
Swapna S Shanbhag, Hajirah N Saeed, Eleftherios I Paschalis, James Chodosh
PURPOSE: To systematically review the published literature on outcomes of Boston keratoprosthesis type 1 for the treatment of limbal stem cell deficiency secondary to severe chemical corneal injury. METHODS: Literature searches were conducted in MEDLINE (Ovid), Embase, Web of Science, and the Cochrane Central Register. The main outcome measures assessed were the proportion of eyes with best-corrected visual acuity (BCVA) ≥ 20/200 and the proportion retaining their original keratoprosthesis, both at the last recorded visit...
March 26, 2018: Ocular Surface
https://www.readbyqxmd.com/read/29576892/amniotic-membrane-transplantation-in-surgical-treatment-of-conjunctival-melanoma-long-term-results
#15
Melis Palamar, Banu Yaman, Taner Akalın, Ayse Yağcı
Objectives: To investigate the long-term efficacy and results of surgical management of conjunctival melanoma reconstructed with amniotic membrane transplantation. Materials and Methods: Conjunctival melanoma in 10 patients (5 female, 5 male) was totally excised with adjunctive cryotherapy to the surgical margins, corneal epitheliectomy with absolute alcohol in cases of corneal involvement, lamellar sclerectomy in cases with episcleral involvement, and ocular surface grafting with cryopreserved amniotic membrane...
February 2018: Turkish Journal of Ophthalmology
https://www.readbyqxmd.com/read/29573222/concise-review-altered-versus-unaltered-amniotic-membrane-as-a-substrate-for-limbal-epithelial-cells
#16
REVIEW
Tor Paaske Utheim, Øygunn Aass Utheim, Panagiotis Salvanos, Catherine J Jackson, Stefan Schrader, Gerd Geerling, Amer Sehic
Limbal stem cell deficiency (LSCD) can result from a variety of corneal disorders, including chemical and thermal burns, infections, and autoimmune diseases. The symptoms of LSCD may include irritation, epiphora, blepharospasms, photophobia, pain, and decreased vision. There are a number of treatment options, ranging from nonsurgical treatments for mild LSCD to various forms of surgery that involve different cell types cultured on various substrates. Ex vivo expansion of limbal epithelial cells (LEC) involves the culture of LEC harvested either from the patient, a living relative, or a cadaver on a substrate in the laboratory...
March 23, 2018: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/29572164/surgical-management-of-severe-ocular-surface-injury-due-to-roman-candle-explosion-accidents
#17
Albert Y Cheung, Brad M Genereux, Brittney Dautremont, Amit Govil, Edward J Holland
PURPOSE: To report the clinical features, management, and outcomes of patients with ocular surface damage secondary to Roman candle explosion accidents. METHODS: Retrospective, noncomparative, interventional case series of 11 patients with Roman candle explosion-related ocular surface injuries referred to the Cincinnati Eye Institute between 2007 and 2016. RESULTS: Eleven patients (10 male, 1 female, mean age 22.4 years) sustained unilateral ocular surface injuries with presenting visual acuity ranging from count fingers to light perception...
March 20, 2018: Ocular Surface
https://www.readbyqxmd.com/read/29572074/advanced-biotherapy-for-the-treatment-of-sulfur-mustard-poisoning
#18
REVIEW
Mingxue Sun, Yuyan Yang, Wenqi Meng, Qingqiang Xu, Fengwu Lin, Yongchun Chen, Jie Zhao, Kai Xiao
CONTEXT: Sulfur mustard (SM), a bifunctional alkylating agent, can react with a variety of biochemical molecules (DNA, RNA, proteins and other cell components) to cause a series of serious health issues or even death. Although a plethora of research has been done, the pathogenesis of SM poisoning has yet to be fully understood due to its high complexity. As a consequence, a specific antidote has not yet been developed and the treatment of SM poisoning remains a medical challenge. In recent years, various biological products and cell transplantation in the treatment of SM poisoning offered a significant clinical treatment progress...
March 20, 2018: Chemico-biological Interactions
https://www.readbyqxmd.com/read/29543561/letter-to-the-editor-type-1-boston-keratoprosthesis-for-limbal-stem-cell-deficiency-in-epidermolysis-bullosa
#19
Milad Modabber, Mona Harissi-Dagher
No abstract text is available yet for this article.
March 15, 2018: Ocular Immunology and Inflammation
https://www.readbyqxmd.com/read/29543204/-surgical-treatment-of-pterygium
#20
A V Petrayevsky, K S Trishkin
Pterygium is one of the modern problems of ophthalmology. The exact etiology and pathogenesis of this disease has not been reliably established and, as a result, there is no universal method for its recurrence-free treatment. The literature data on various methods of pterygium surgical treatment such as 'bare sclera', conjunctival autograft, limbal stem cells transplantation techniques, amniotic membrane plasty and other methods was analyzed. The advantages and disadvantages of those surgery methods were identified...
2018: Vestnik Oftalmologii
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