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Limbal stem cell

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https://www.readbyqxmd.com/read/28926502/limbal-cysts-a-subset-exhibiting-cornea-specific-cytokeratins
#1
Norman C Charles, Frederick A Jakobiec, Fouad R Zakka, Ilyse D Haberman, Kishore Reddy Katikireddy, Ula V Jurkunas
Two cases of limbal cysts lined by nonkeratinizing epithelium were studied with a panel of cytokeratins. One was a long-standing lesion in a 30-year-old man, whereas the other was excised from a 40-year-old man following pterygium surgery. Each cyst was immunostained with a panel of cytokeratins that were specific exclusively and separately for corneal and conjunctival epithelia. The epithelial lining of each cyst was CK12 positive for corneal epithelium and CK13 negative for conjunctival epithelium. It is hypothesized that a subset of corneoscleral cysts contain corneal epithelium, probably derived from a type of limbal stem cell differentiation...
September 13, 2017: Ophthalmic Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28922332/evaluation-of-corneal-neovascularization-using-optical-coherence-tomography-angiography-in-patients-with-limbal-stem-cell-deficiency
#2
Yoshinori Oie, Kohji Nishida
PURPOSE: Detection of the exact area of corneal neovascularization using slit-lamp photography is often difficult. Thus, we evaluated corneal neovascularization in patients with limbal stem cell deficiency using optical coherence tomography angiography (OCTA). METHODS: Five patients with 5 eyes showing partial or total limbal stem cell deficiency were enrolled. Three eyes had severe corneal scarring. Five 6- × 6-mm images (frontal, upper, lower, nasal, and temporal) were obtained by OCTA...
September 14, 2017: Cornea
https://www.readbyqxmd.com/read/28906020/diagnostic-impact-of-anterior-segment-angiography-of-limbal-stem-cell-insufficiency-in-pax6-related-aniridia
#3
Barbara Käsmann-Kellner, Lorenz Latta, Fabian Fries, Arne Viestenz, Berthold Seitz
BACKGROUND: PAX6 is a master gene of ocular development and postnatal ocular equilibrium. Congenital aniridia is the hallmark of PAX6 gene haploinsufficiency (Chr. 11 p. 13), but PAX6-associated aniridia is a profound, progressive pan-ocular developmental disorder often leading to blindness. Limbal stem cell insufficiency and corneal findings in PAX6 syndrome: There is congenital visual impairment with advancing loss of vision mainly due to secondary glaucoma and to corneal blindness caused by limbal stem cell insufficiency (LSCI)...
September 14, 2017: Clinical Anatomy
https://www.readbyqxmd.com/read/28898916/-rehabilitation-following-limbal-stem-cell-deficiency-current-treatment-options-and-future-developments
#4
Henning Thomasen, Klaus P Steuhl, Daniel Meller
Limbal stem cell deficiency (LSCD) is a condition caused by the loss of corneal epithelial regenerative potential. The treatment of this condition is still a challenge. It results from various conditions both intrinsic as well as extrinsic. LSCD can be either uni- or bilateral and either partial or total. Today treatment options include a variety of techniques including transplantation of amniotic membrane and limbal tissue or tissue engineered cell sheets. This article summarizes the current techniques to treat LSCD and upcoming developments...
September 12, 2017: Klinische Monatsblätter Für Augenheilkunde
https://www.readbyqxmd.com/read/28894469/ex-vivo-expansion-of-human-limbal-epithelial-cells-using-human-placenta-derived-and-umbilical-cord-derived-mesenchymal-stem-cells
#5
Sang Min Nam, Yong-Sun Maeng, Eung Kweon Kim, Kyoung Yul Seo, Helen Lew
Ex vivo culture of human limbal epithelial cells (LECs) is used to treat limbal stem cell (LSC) deficiency, a vision loss condition, and suitable culture systems using feeder cells or serum without animal elements have been developed. This study evaluated the use of human umbilical cord or placenta mesenchymal stem cells (C-MSCs or P-MSCs, resp.) as feeder cells in an animal/serum-free coculture system with human LECs. C-/P-MSCs stimulated LEC colony formation of the stem cell markers (p63, ABCG2) and secreted known LEC clonal growth factors (keratinocyte growth factor, β-nerve growth factor)...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28878217/in-vivo-confocal-microscopy-evaluation-of-ocular-surface-with-graft-versus-host-disease-related-dry-eye-disease
#6
Jingliang He, Yoko Ogawa, Shin Mukai, Yumiko Saijo-Ban, Mizuka Kamoi, Miki Uchino, Mio Yamane, Nobuhiro Ozawa, Masaki Fukui, Takehiko Mori, Shinichiro Okamoto, Kazuo Tsubota
Dry eye disease (DED) is often elicited by graft-versus-host disease (GVHD), an extensive complication of hematopoietic stem cell transplantation (HSCT). To unravel the mechanism of this type of DED, in vivo confocal microscopy (IVCM) was used to investigate alterations in the state of the sub-basal nerves, dendritic cells (DCs) and globular immune cells (GICs) in the central cornea and limbal epithelia. In this study, we examined 12 HSCT recipients with GVHD-caused DED and 10 HSCT recipients without GVHD-associated DED and evaluated the clinical parameters in the 2 groups...
September 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28863216/hyaluronan-rich-microenvironment-in-the-limbal-stem-cell-niche-regulates-limbal-stem-cell-differentiation
#7
Tarsis F Gesteira, Mingxia Sun, Yvette M Coulson-Thomas, Yu Yamaguchi, Lung-Kun Yeh, Vincent Hascall, Vivien J Coulson-Thomas
Purpose: Limbal epithelial stem cells (LSCs), located in the basal layer of the corneal epithelium in the corneal limbus, are vital for maintaining the corneal epithelium. LSCs have a high capacity of self-renewal with increased potential for error-free proliferation and poor differentiation. To date, limited research has focused on unveiling the composition of the limbal stem cell niche, and, more important, on the role the specific stem cell niche may have in LSC differentiation and function...
September 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28860936/stem-cells-in-regenerative-medicine-from-laboratory-to-clinical-application-the-eye
#8
REVIEW
Anna M Dąbrowska, Piotr Skopiński
Stem cells are currently one of the most researched and explored subject in science. They consstitue a very promising part of regenerative medicine and have many potential clinical applications. Harnessing their ability to replicate and differentiate into many cell types can enable successful treatment of diseases that were incurable until now. There are numerous types of stem cells (e.g. ESCs, FSCs, ASCs, iPSCs) and many different methods of deriving and cultivating them in order to obtain viable material...
2017: Central-European Journal of Immunology
https://www.readbyqxmd.com/read/28855664/oral-mucosal-epithelial-cells-grown-on-porous-silicon-membrane-for-transfer-to-the-rat-eye
#9
Yazad D Irani, Sonja Klebe, Steven J P McInnes, Marek Jasieniak, Nicolas H Voelcker, Keryn A Williams
Dysfunction of limbal stem cells or their niche can result in painful, potentially sight-threatening ocular surface disease. We examined the utility of surface-modified porous-silicon (pSi) membranes as a scaffold for the transfer of oral mucosal cells to the eye. Male-origin rat oral mucosal epithelial cells were grown on pSi coated with collagen-IV and vitronectin, and characterised by immunocytochemistry. Scaffolds bearing cells were implanted into normal female rats, close to the limbus, for 8 weeks. Histology, immunohistochemistry and a multiplex nested PCR for sry were performed to detect transplanted cells...
August 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28844373/blood-derived-eye-drops-for-the-treatment-of-cornea-and-ocular-surface-diseases
#10
REVIEW
Giuseppe Giannaccare, Piera Versura, Marina Buzzi, Laura Primavera, Marco Pellegrini, Emilio C Campos
The use of blood derived eye drops for the treatment of ocular surface disorders has become increasingly popular in recent years. The mechanism of action is the stimulation of cellular proliferation and migration by supplying an active mixture of growth factors and cytokines at the ocular surface, thus mimicking the function of the lacking natural tears. Blood derived eye drops have been used in the last decades for the treatment of a variety of ocular surface diseases, including mainly dry eye disease, persistent corneal epithelial defect, corneal ulcer, ocular surface burn, recurrent corneal erosion and limbal stem-cell deficiency...
August 8, 2017: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/28834816/donor-derived-conjunctival-limbal-melanoma-after-a-keratolimbal-allograft
#11
Lorena Sepsakos, Albert Y Cheung, Jeffrey A Nerad, Gautham Mogilishetty, Edward J Holland
PURPOSE: To report a single case of donor-derived conjunctival-limbal melanoma that occurred after a keratolimbal allograft (KLAL). METHODS: Case report and literature review. RESULTS: A 56-year-old white woman with a history of bilateral limbal stem cell deficiency developed a donor-related melanoma after a KLAL. Three months after undergoing an uncomplicated KLAL, the patient presented with hemorrhagic nodules within her conjunctiva and transplanted tissue...
August 22, 2017: Cornea
https://www.readbyqxmd.com/read/28827194/midterm-outcomes-of-penetrating-keratoplasty-after-cultivated-oral-mucosal-epithelial-transplantation-in-chemical-burn
#12
Alireza Baradaran-Rafii, Siamak Delfazayebaher, Nasser Aghdami, Ehsan Taghiabadi, Shahram Bamdad, Danial Roshandel
PURPOSE: To evaluate the mid-term outcome of penetrating keratoplasty (PKP) after cultivated oral mucosal epithelial transplantation (COMET) in patients with bilateral total limbal stem cell deficiency (LSCD) due to chemical burn. METHODS: In this prospective interventional nonrandomized case series, optical PKP was performed in patients with severe stromal opacity after successful COMET. Main outcome measures were stability of the ocular surface, visual acuity improvement and corneal graft survival...
August 18, 2017: Ocular Surface
https://www.readbyqxmd.com/read/28816624/comparison-of-two-rabbit-models-with-deficiency-of-corneal-epithelium-and-limbal-stem-cells-established-by-different-methods
#13
Hening Zhang, Shaochun Lin, Min Zhang, Qijiong Li, Weihua Li, Wencong Wang, Minglei Zhao, Yaojue Xie, Zhiquan Li, Mian Huang, Zhichong Wang, Xiulan Zhang, Bing Huang
Limbal stem cell defect model is an important animal model that provides a basis for the study of ocular surface diseases. The rabbit cornea is of moderate size and is widely used in such studies as an experimental animal model. At present, the main modeling methods are alkali burns, and corneal limbus girdling and corneal epithelium doctoring. Each method has its own characteristics. In this study, we observed rabbit models with severe ocular surface defect established by the two methods and changes after amniotic membrane transplantation...
August 14, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28813511/differentiation-of-human-embryonic-stem-cells-into-corneal-epithelial-progenitor-cells-under-defined-conditions
#14
Canwei Zhang, Liqun Du, Kunpeng Pang, Xinyi Wu
The development of cell-based therapies using stem cells represents a significant breakthrough in the treatment of limbal stem cell deficiency (LSCD). The aim of this study was to develop a novel protocol to differentiate human embryonic stem cells (hESCs) into corneal epithelial progenitor cells (CEPCs), with similar features to primary cultured human limbal stem cells (LSCs), using a medium composed of DMEM/F12 and defined keratinocyte serum-free medium (KSFM) (1:1) under different carbon dioxide (CO2) levels in culture...
2017: PloS One
https://www.readbyqxmd.com/read/28810264/mesenchymal-stem-cells-promote-diabetic-corneal-epithelial-wound-healing-through-tsg-6-dependent-stem-cell-activation-and-macrophage-switch
#15
Guohu Di, Xianli Du, Xia Qi, Xiaowen Zhao, Haoyun Duan, Suxia Li, Lixin Xie, Qingjun Zhou
Purpose: To explore the role and mechanism of bone marrow-derived mesenchymal stem cells (BM-MSCs) in corneal epithelial wound healing in type 1 diabetic mice. Methods: Diabetic mice were treated with subconjunctival injections of BM-MSCs or recombinant tumor necrosis factor-α-stimulated gene/protein-6 (TSG-6). The corneal epithelial wound healing rate was examined by fluorescein staining. The mRNA and protein expression levels of TSG-6 were measured by quantitative RT-PCR and Western blot...
August 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28780431/thermosensitive-chitosan-based-hydrogels-releasing-stromal-cell-derived-factor-1-alpha-recruit-msc-for-corneal-epithelium-regeneration
#16
Qiaomei Tang, Chenqi Luo, Bing Lu, Qiuli Fu, Houfa Yin, Zhenwei Qin, Danni Lyu, Lifang Zhang, Zhi Fang, Yanan Zhu, Ke Yao
Corneal epithelium integrity depends on continuous self-renewing of epithelium and connections between adjacent cells or between the cells and the basement membrane. Self-renewing epithelium cells mainly arise from the continuous proliferation and differentiation of the basal layer and limbal stem cells. The aim of the present study was to generate a bioactive, thermosensitive chitosan-gelatin hydrogel (CHI hydrogel) by incorporating exogenous recombinant human stromal cell-derived factor-1 alpha (SDF-1 alpha) for corneal epithelium regeneration...
August 2, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28760619/gamma-irradiated-human-amniotic-membrane-decellularised-with-sodium-dodecyl-sulfate-is-a-more-efficient-substrate-for-the-ex-vivo-expansion-of-limbal-stem-cells
#17
G S Figueiredo, S Bojic, P Rooney, S-P Wilshaw, C J Connon, R M Gouveia, C Paterson, G Lepert, H S Mudhar, F C Figueiredo, M Lako
The gold standard substrate for the ex vivo expansion of human limbal stem cells (LSCs) remains the human amniotic membrane (HAM) but this is not a defined substrate and is subject to biological variability and the potential to transmit disease. To better define HAM and mitigate the risk of disease transmission, we sought to determine if decellularisation and/or γ-irradiation have an adverse effect on culture growth and LSC phenotype. Ex vivo limbal explant cultures were set up on fresh HAM, HAM decellularised with 0...
July 29, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28758321/therapeutic-effect-of-human-adipose-tissue-derived-mesenchymal-stem-cells-in-experimental-corneal-failure-due-to-limbal-stem-cell-niche-damage
#18
Sara Galindo, José M Herreras, Marina López-Paniagua, Esther Rey, Ana de la Mata, María Plata-Cordero, Margarita Calonge, Teresa Nieto-Miguel
Limbal stem cells are responsible for the continuous renewal of the corneal epithelium. The destruction or dysfunction of these stem cells or their niche induces limbal stem cell deficiency (LSCD) leading to visual loss, chronic pain, and inflammation of the ocular surface. To restore the ocular surface in cases of bilateral LSCD, an extraocular source of stem cells is needed to avoid dependence on allogeneic limbal stem cells that are difficult to obtain, isolate, and culture. The aim of this work was to test the tolerance and the efficacy of human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) to regenerate the ocular surface in two experimental models of LSCD that closely resemble different severity grades of the human pathology...
July 31, 2017: Stem Cells
https://www.readbyqxmd.com/read/28743889/limbal-stromal-cells-derived-from-porcine-tissue-demonstrate-mesenchymal-characteristics-in-vitro
#19
Julia Fernández-Pérez, Marcus Binner, Carsten Werner, Laura J Bray
Limbal stromal cells (LSCs) from the human ocular surface display mesenchymal stromal cell characteristics in vitro. In this study, we isolated cells from the porcine limbal stroma (pLSCs), characterised them, and evaluated their ability to support angiogenesis and the culture of porcine limbal epithelial stem cells (pLESCs). The isolated cells adhered to plastic and grew in monolayers in vitro using serum-supplemented or serum-free medium. The pLSCs demonstrated expression of CD29, and cross-reactivity with anti-human CD45, CD90, CD105, CD146, and HLA-ABC...
July 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28731936/case-series-keratolimbal-allograft-as-a-patch-graft-for-glaucoma-drainage-devices
#20
Sarah F Ahmed, Mason Schmutz, Sameh Mosaed
PURPOSE: Tube exposure remains one of the most common complications after glaucoma drainage device (GDD) implantation, despite various types of patch grafts available today. We present a 4 patient case series following the effectivity of the keratolimbal allograft (KLAL) as a patch graft for cases of tube exposure. Given its inherent population of stem cells, our hypothesis was that this highly replicative, biological tissue would provide an adequate means of glaucoma tube coverage. The subset of patients chosen for the KLAL patch graft all had a history of abnormally scarred conjunctiva or thin sclera...
July 20, 2017: Journal of Glaucoma
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