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https://www.readbyqxmd.com/read/27911390/a-method-for-extracting-pigments-from-squid-doryteuthis-pealeii
#1
Christopher W DiBona, Thomas L Williams, Sean R Dinneen, Stephanie F Jones Labadie, Leila F Deravi
Cephalopods can undergo rapid and adaptive changes in dermal coloration for sensing, communication, defense, and reproduction purposes. These capabilities are supported in part by the areal expansion and retraction of pigmented organs known as chromatophores. While it is known that the chromatophores contain a tethered network of pigmented granules, their structure-function properties have not been fully detailed. We describe a method for isolating the nanostructured granules in squid Doryteuthis pealeii chromatophores and demonstrate how their associated pigments can be extracted in acidic solvents...
November 9, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27911021/a-rapid-and-simple-liquid-chromatography-tandem-mass-spectrometry-method-for-the-measurement-of-testosterone-androstenedione-and-dehydroepiandrosterone-in-human-serum
#2
Wenjuan Xu, Huijun Li, Qing Guan, Ying Shen, Liming Cheng
BACKGROUND: The assay of androgens plays an important role for the differential diagnosis of androgen-related endocrine diseases. We developed a rapid and simple liquid chromatography tandem mass spectrometry (LC-MS/MS) method with small sample volume and simple sample preparation for simultaneous determination of serum testosterone, androstenedione and dehydroepiandrosterone (DHEA) in adults. METHODS: We spiked 100 μL of serum with 200 μL of internal standard solution, which was prepared in methanol, for matrix effect relief and protein precipitation...
December 2, 2016: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/27910970/exploration-of-hydrogen-bond-networks-and-potential-energy-surfaces-of-methanol-clusters-using-a-two-stage-clustering-algorithm
#3
Po-Jen Hsu, Kun-Lin Ho, Sheng-Hsien Lin, Jer-Lai Kuo
The potential energy surface (PES), structures and thermal properties of methanol clusters (MeOH)n with n = 8-15 were explored by replica-exchange molecular dynamics (REMD) simulations with an empirical model and refined using density functional theory (DFT) methods. For a given size, local minima structures were sampled from REMD trajectories and archived by a newly developed molecular database via a two-stage clustering algorithm (TSCA). Our TSCA utilizes both the topology of O-HO hydrogen bonding networks and the similarity of the shapes to filter out duplicates...
December 2, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27910773/giant-oyster-mushroom-pleurotus-giganteus-agaricomycetes-enhances-adipocyte-differentiation-and-glucose-uptake-via-activation-of-ppar%C3%AE-and-glucose-transporters-1-and-4-in-3t3-l1-cells
#4
Puvaneswari Paravamsivam, Chua Kek Heng, Sri Nurestri Abdul Malek, Vikineswary Sabaratnam, Ravishankar Ram M, Umah Rani Kuppusamy
The edible mushroom Pleurotus giganteus was tested for its effect on adipocyte differentiation and glucose uptake activity in 3T3-L1 cells. The basidiocarps of P. giganteus were soaked in methanol to obtain a crude methanol extract and then fractionated to obtain an ethyl acetate extract. In this study, cell proliferation was measured using an MTT assay, lipid accumulation using an Oil Red O assay, and glucose uptake using a fluorescence glucose uptake assay. Gene expression was measured via real-time polymerase chain reaction analysis with TaqMan primer...
2016: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/27910224/separating-and-identifying-the-four-stereoisomers-of-methyl-jasmonate-by-rp-hplc-and-using-cyclodextrins-in-a-novel-way
#5
Adrián Matencio, Mario J Bermejo-Gimeno, Francisco García-Carmona, José Manuel López-Nicolás
INTRODUCTION: Several authors have reported on the different bioactivities of methyl jasmonate (MeJA) stereoisomers. However, no simple, precise and cheap method for separating and identifying them using reversed-phase high performance liquid chromatography (RP-HPLC) has been developed. OBJECTIVE: (1) To create a simple, precise and cheap method for separating and identifying the four stereoisomers present in commercial racemic mixtures of MeJA and (2) to identify the four stereoisomers using molecular docking techniques and coinjection...
December 2, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27910199/reverse-phase-compatible-tlc-bioautography-for-detection-of-tyrosinase-inhibitors
#6
Paula García, I Ayelen Ramallo, Ricardo L E Furlan
INTRODUCTION: Reverse phase chromatography and bioautographic assays are key tools for natural product bioguided isolation; however, their direct coupling has not been fully achieved. OBJECTIVES: To develop a bioautographic assay to detect tyrosinase inhibitors present in complex matrices sorbed on reverse phase (RP) TLC-plates that can be used for bioguided isolation of bioactive compounds. METHODS: Enzyme gel entrapment with an amphiphilic copolymer was used for assay development...
December 2, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27910197/a-stable-manganese-pincer-catalyst-for-the-selective-dehydrogenation-of-methanol
#7
María Andérez-Fernández, Lydia K Vogt, Steffen Fischer, Wei Zhou, Haijun Jiao, Marcel Garbe, Saravanakumar Elangovan, Kathrin Junge, Henrik Junge, Ralf Ludwig, Matthias Beller
For the first time, structurally defined manganese pincer complexes catalyze the dehydrogenation of aqueous methanol to hydrogen and carbon dioxide, which is a transformation of interest with regard to the implementation of a hydrogen and methanol economy. Excellent long-term stability was demonstrated for the Mn-PNPiPr catalyst, as a turnover of more than 20 000 was reached. In addition to methanol, other important hydrogen carriers were also successfully dehydrogenated.
December 2, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/27910164/hplc-uv-and-lc-ms-analyses-of-acylquinic-acids-in-geigeria-alata-dc-oliv-hiern-and-their-contribution-to-antioxidant-and-antimicrobial-capacity
#8
Dimitrina Zheleva-Dimitrova, Reneta Gevrenova, Maya M Zaharieva, Hristo Najdenski, Silviya Ruseva, Valentin Lozanov, Vessela Balabanova, Sakina Yagi, Georgi Momekov, Vanio Mitev
INTRODUCTION: Geigeria alata is a traditional plant used in Sudanese folk medicine for treatment of diabetes, cough, epilepsy and intestinal complaints. OBJECTIVE: To analyze phenolic acids in Geigeria alata roots and leaves and to evaluate their antioxidant and antimicrobial activities. METHODOLOGY: Phenolic acids in the aqueous-methanol extracts were identified by LC-MS. Major compounds were isolated using low-pressure liquid chromatography...
December 1, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27910158/hptlc-bioautography-guided-isolation-of-%C3%AE-glucosidase-inhibiting-compounds-from-justicia-secunda-vahl-acanthaceae
#9
Barbara A Theiler, Stefanie Istvanits, Martin Zehl, Laurence Marcourt, Ernst Urban, Lugardo O Espinoza Caisa, Sabine Glasl
INTRODUCTION: α-Glucosidase inhibitors form an essential basis for the development of novel drugs in diabetes type 2 treatment. Searching for α-glucosidase inhibitors in plants, TLC bioautographic assays have been established and improved within the last years. In traditional medicine, extracts from the leaves of Justicia secunda Vahl are used to treat diabetes mellitus symptoms. OBJECTIVE: To screen for α-glucosidase inhibitors in J. secunda via HPTLC bioautography...
December 2, 2016: Phytochemical Analysis: PCA
https://www.readbyqxmd.com/read/27909778/it-is-all-about-the-solvent-on-the-importance-of-the-mobile-phase-for-zic-hilic-glycopeptide-enrichment
#10
Kathirvel Alagesan, Sana Khan Khilji, Daniel Kolarich
Glycopeptide enrichment is a crucial step in glycoproteomics for which hydrophilic interaction chromatography (HILIC) has extensively been applied due to its low bias towards different glycan types. A systematic evaluation of applicable HILIC mobile phases on glycopeptide enrichment efficiency and selectivity is, to date, however, still lacking. Here, we present a novel, simplified technique for HILIC enrichment termed "Drop-HILIC", which was applied to systematically evaluate the mobile phase effect on ZIC-HILIC (zwitterionic type of hydrophilic interaction chromatography) glycopeptide enrichment...
December 1, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27908201/cocos-nucifera-coir-mediated-green-synthesis-of-pd-nps-and-its-investigation-against-larvae-and-agricultural-pest
#11
Ganesh Elango, Selvaraj Mohana Roopan, Naif Abdullah Al-Dhabi, Mariadhas Valan Arasu, Kasinathan Irukatla Damodharan, Kuppuswamy Elumalai
In recent decades, several scientists focused their process towards nanoparticles synthesis by using various sustainable approaches. Cocos nucifera (C. nucifera) was one of the versatile trees in tropical regions which also can act as a thrust quencher in all over the world. Cocos nucifera coir was one of the waste by-products in all coconut-refining industries and with the help C. nucifera coir, Palladium nanoparticles (Pd NPs) were synthesized. Green-synthesized spherical-shape Pd NPs were over layered by secondary metabolites from C...
December 1, 2016: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/27907855/lc-ms-ms-assay-for-the-quantitation-of-the-tyrosine-kinase-inhibitor-neratinib-in-human-plasma
#12
Brian F Kiesel, Robert A Parise, Alvin Wong, Kiana Keyvanjah, Samuel Jacobs, Jan H Beumer
Neratinib is an orally available tyrosine kinase inhibitor targeting HER2 (ERBB2) and EGFR (ERBB). It is being clinically evaluated for the treatment of breast and other solid tumors types as a single agent or in combination with other chemotherapies. In support of several phase I/II clinical trials investigating neratinib combinations, we developed and validated a novel LC-MS/MS assay for the quantification of neratinib in 100μL of human plasma with a stable isotopic internal standard. Analytes were extracted from plasma using protein precipitation and evaporation of the resulting supernatant followed by resuspension...
November 22, 2016: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/27907844/oxidation-of-atrazine-in-aqueous-media-by-solar-enhanced-fenton-like-process-involving-persulfate-and-ferrous-ion
#13
Marina Khandarkhaeva, Agniya Batoeva, Denis Aseev, Marina Sizykh, Oyuna Tsydenova
The oxidation of s-triazines (using atrazine (ATZ) as a model compound) by a solar-enhanced Fenton-like process involving persulfate and ferrous ion was studied. A flow-through tubular photoreactor was employed for the experiments. The solar-enhanced oxidative system involving ferrous ion and persulfate (Solar/S2O8(2-)/Fe(2+)) showed the highest ATZ degradation efficiency when compared with other treatments (unactivated S2O8(2-), Solar - sunlight only, S2O8(2-)/Fe(2+), Solar/S2O8(2-)). Complete degradation of ATZ and 20% reduction in total organic carbon (TOC) content were observed after 30min of the treatment...
November 28, 2016: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/27907132/a-simplified-and-efficient-process-for-insulin-production-in-pichia-pastoris
#14
Sulena Polez, Domenico Origi, Sotir Zahariev, Corrado Guarnaccia, Sergio G Tisminetzky, Nataša Skoko, Marco Baralle
A significant barrier to insulin is affordability. In this manuscript we describe improvements to key steps in the insulin production process in Pichia pastoris that reduce cost and time. The strategy for recovery and processing of human insulin precursor has been streamlined to two steps from bioreactor to the transpeptidation reaction. In the first step the insulin precursor secreted during the methanol induction phase is recovered directly from the culture broth using Tangential Flow Filtration with a Prostak™ module eliminating the laborious and time-consuming multi-step clarification, including centrifugation...
2016: PloS One
https://www.readbyqxmd.com/read/27906402/one-step-synthesis-of-ultrathin-ptxpb-nerve-like-nanowires-as-robust-catalysts-for-enhanced-methanol-electrooxidation
#15
Liang Huang, Yujie Han, Xueping Zhang, Youxing Fang, Shaojun Dong
Ultrathin PtxPb nerve-like nanowires (NNWs) with a diameter of only around 3.6 nm were synthesized by a one-step wet-chemical strategy, and they served as robust catalysts for greatly enhancing methanol electrooxidation both under acidic and alkaline conditions. Due to the high CO-poisoning tolerance, superior electrocatalytic activity and stability endowed by the Pt-Pb alloyed composition and the unique structure, the Pt3.5Pb NNWs showed the highest specific activity of 2.78 mA cm(-2) in acidic media and 6...
December 1, 2016: Nanoscale
https://www.readbyqxmd.com/read/27906138/larvicidal-activity-of-green-synthesized-silver-nanoparticles-using-excoecaria-agallocha-l-euphorbiaceae-leaf-extract-against-aedes-aegypti
#16
Vundru Anil Kumar, Kandru Ammani, Rajkumari Jobina, Paramanandham Parasuraman, Busi Siddhardha
Green synthesis of silver nanoparticles (AgNPs) using plant extracts has been achieved by eco-friendly reducing and capping agents. The present study was conducted to evaluate the larvicidal efficacies of AgNPs synthesized using aqueous leaf extracts of Excoecaria agallocha against dengue vector, Aedes aegypti. The 3(rd) and 4(th) instar larvae of A. aegypti were exposed to various concentrations of aqueous extracts of E. agallocha, synthesized AgNPs and also crude solvent extracts (methanol and chloroform) for 24 h...
December 2016: IET Nanobiotechnology
https://www.readbyqxmd.com/read/27905591/ceo2-modified-%C3%AE-moo3-nanorods-as-a-synergistic-support-for-pt-nanoparticles-with-enhanced-coads-tolerance-during-methanol-oxidation
#17
Yanying Liu, Chuntao Liu, Xuefeng Yu, Hannah Osgood, Gang Wu
A new type of Ce-doped α-MoO3 (Ce0.2Mo0.8O3-δ) nanorod support was synthesized using a two-step hydrothermal method. The mixed oxide solid solution was employed as a novel non-carbon support for Pt catalysts used for the methanol electrooxidation in acidic media. The ratio of Ce to Mo in the solid-solution was systematically optimized in terms of the performance as a support for methanol oxidation. Pt nanoparticles with an average diameter of 2 nm were evenly deposited on Ce0.2Mo0.8O3-δ nanorods. Compared to the Pt/MoO3 and commercial Pt/C catalysts, the optimal Pt/Ce0...
December 1, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27905552/protonation-enhancement-by-dichloromethane-doping-in-low-pressure-photoionization
#18
Jinian Shu, Yao Zou, Ce Xu, Zhen Li, Wanqi Sun, Bo Yang, Haixu Zhang, Peng Zhang, Pengkun Ma
Doping has been used to enhance the ionization efficiency of analytes in atmospheric pressure photoionization, which is based on charge exchange. Compounds with excellent ionization efficiencies are usually chosen as dopants. In this paper, we report a new phenomenon observed in low-pressure photoionization: Protonation enhancement by dichloromethane (CH2Cl2) doping. CH2Cl2 is not a common dopant due to its high ionization energy (11.33 eV). The low-pressure photoionization source was built using a krypton VUV lamp that emits photons with energies of 10...
December 1, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27904047/protective-effect-of-deer-bone-oil-on-cartilage-destruction-in-rats-with-monosodium-iodoacetate-mia-induced-osteoarthritis
#19
Hyeon-Son Choi, Suji Im, Je Won Park, Hyung Joo Suh
The anti-osteoarthritic activity of the methanol fraction of deer bone oil extract (DBO-M) was evaluated in interleukin (IL)-1β-inflamed primary rabbit chondrocytes and in rats with monosodium iodoacetate (MIA)-induced osteoarthritis. The active compound in DBO-M was analyzed using a direct infusion liquid chromatography quadrupole (LCQ) ion-trap electrospray ionization (ESI)-mass spectrometer (MS). DBO-M significantly suppressed the IL-1β-induced sulfated-glycosaminoglycan (s-GAG) release from chondrocyte, and lowered mRNA levels of the collagen-degrading enzymes matrix metalloproteinase (MMP)-1 and MMP-3 in a dose-dependent manner...
2016: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/27903992/a-brief-review-on-toxic-alcohols-management-strategies
#20
Hossein Hassanian-Moghaddam, Nasim Zamani
The information on burden of alcohol abuse in Iran is scarce. However, the available data show that mortality rates and frequency of its use have increased in the Iranian community. In particular, Iran occupies the 1st rank in the number of outbreak incidents and victims of toxic alcohols such as methanol in the Middle East. Mortality and morbidity of toxic alcohols are high if prompt diagnosis and treatment are not initiated rapidly. On-time diagnosis, proper case finding, and standard treatment have an essential role to reduce mortality and morbidity of toxic alcohols particularly blindness and other physical and psychological disabilities...
November 2016: Iranian Journal of Kidney Diseases
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