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https://www.readbyqxmd.com/read/29454253/charge-transfer-complex-between-2-3-diaminopyridine-with-chloranilic-acid-synthesis-characterization-and-dft-td-dft-computational-studies
#1
Khairia M Al-Ahmary, Moustafa M Habeeb, Areej H Al-Obidan
New charge transfer complex (CTC) between the electron donor 2,3-diaminopyridine (DAP) with the electron acceptor chloranilic (CLA) acid has been synthesized and characterized experimentally and theoretically using a variety of physicochemical techniques. The experimental work included the use of elemental analysis, UV-vis, IR and 1 H NMR studies to characterize the complex. Electronic spectra have been carried out in different hydrogen bonded solvents, methanol (MeOH), acetonitrile (AN) and 1:1 mixture from AN-MeOH...
February 10, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29453917/comparative-chemical-investigation-and-evaluation-of-antioxidant-and-tyrosinase-inhibitory-effects-of-withania-somnifera-l-dunal-and-solanum-nigrum-l-berries
#2
Abdul Hameed, Naveed Akhtar
In the present study, berries of two different species of Solanaceae family, Withania somnifera (WS) and Solanum nigrum (SN), were extracted in methanol and then fractionated with solvents, ranging from non-polar to polar, for their phytochemical profiling and investigation of antioxidant and tyrosinase enzyme inhibition capacity. The methanolic extract and n-hexane, ethyl acetate (WSEA, SNEA) and aqueous fractions were chemically analyzed and evaluated for biological activity. Total flavonoids and total phenolics were quantified in WSEA (96...
March 1, 2018: Acta Pharmaceutica
https://www.readbyqxmd.com/read/29453912/hptlc-determination-of-diosgenin-in-fenugreek-seeds
#3
Barbara Król-Kogus, Khenifi Mohammed Lamine, Piotr Migas, Messaoud Boudjeniba, Mirosława Krauze-Baranowska
A new HPTLC-densitometric method for diosgenin determination in fenugreek seeds was established after optimization of the conditions for efficient saponin extraction and acid hydrolysis. Several procedures were tested, the best of which was a three-step Soxhlet extraction, followed by hydrolysis of the obtained methanolic extract with 2 mol L-1 H2SO4. Best diosgenin separation from other hydrolysis products was obtained on HPTLC Si60F254 plates u sing a mixture of n-heptane/ethyl acetate (7:3, V/V) and modified anisaldehyde as a spraying reagent...
March 1, 2018: Acta Pharmaceutica
https://www.readbyqxmd.com/read/29453649/reinereins-a-and-b-new-onocerane-triterpenoids-from-reinwardtiodendron-cinereum
#4
Alfarius Eko Nugroho, Daisuke Inoue, Chin Piow Wong, Yusuke Hirasawa, Toshio Kaneda, Osamu Shirota, A Hamid A Hadi, Hiroshi Morita
Bioactivity guided separation of Reinwardtiodendron cinereum barks methanol extract led to the isolation of two new onocerane triterpenoids, reinereins A and B (1 and 2), together with three known onocerane triterpenoids. Their structures were elucidated on the basis of NMR spectroscopic data. In vitro cytotoxic activities of the isolated compounds against several type of cancer cells were evaluated.
February 16, 2018: Journal of Natural Medicines
https://www.readbyqxmd.com/read/29453373/dormancy-release-and-germination-of-taxus-yunnanensis-seeds-during-wet-sand-storage
#5
Fangyuan Bian, Jianrong Su, Wande Liu, Shuaifeng Li
Dormancy is an innate constraint on germination that occurs across all life forms. In this study, we investigated the seed dormancy release and germination characters of Taxus yunnanensis by exploring the seed morphology, permeability, germination inhibitors, endogenous hormones, and embryo germination in vitro during wet sand storage. Our results showed that seeds and embryos grew to a critical size to germination and permeability increased with the extension of storage. Seed coat and kernel methanol extracts reduced Brassica campestris seed vigor index...
February 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29453063/-1-h-nmr-based-metabolomics-reveals-interactive-effects-between-the-carrier-solvent-methanol-and-a-pharmaceutical-mixture-in-an-amphibian-developmental-bioassay-with-limnodynastes-peronii
#6
Steven D Melvin, Oliver A H Jones, Anthony R Carroll, Frederic D L Leusch
Organic carrier solvents are used in aquatic toxicity testing to improve chemical solubility and facilitate the exploration of dose-response relationships. Both water- and solvent-control groups are normally included in these scenarios to ensure that the solvent itself has no effect on the test organism, but this fails to consider possible interactive effects between carrier solvents and contaminants of interest. We explored this topic by exposing Limnodynastes peronii tadpoles to a mixture of common water-soluble pharmaceuticals (diclofenac, metformin and valproic acid) in the presence and absence of the carrier solvent methanol, according to standard developmental bioassay methodology...
February 10, 2018: Chemosphere
https://www.readbyqxmd.com/read/29453015/metabolomic-profiling-of-campylobacter-jejuni-with-resistance-gene-ermb-by-ultra-high-performance-liquid-chromatography-quadrupole-time-of-flight-mass-spectrometry-and-tandem-quadrupole-mass-spectrometry
#7
Qin Fu, Dejun Liu, Yingyu Wang, Xiaowei Li, Lina Wang, Fugen Yu, Jianzhong Shen, Xi Xia
The metabolome changes of Campylobacter jejuni with resistant gene ermB remain unclear. Here, we described an untargeted metabolomic workflow based on ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry to investigate the metabolites perturbations mediated by ermB in C. jejuni. After optimization of extractants and chromatographic conditions, the combination of 100% methanol extraction with a 12 min gradient by C18 column was adopted for untargeted metabolomic profiling in reversed phase separation...
February 10, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29452270/high-level-extracellular-production-of-rhizopus-oryzae-lipase-in-pichia-pastoris-via-a-strategy-combining-optimization-of-gene-copy-number-with-co-expression-of-erad-related-proteins
#8
Liangcheng Jiao, Qinghua Zhou, Zhixin Xu, Li Xu, Yunjun Yan
Rhizopus oryzae lipase (ROL) is an important industrial enzyme limited in application due to its low production in native strains. Here, we used a new combined strategy to overexpress ROL in Pichia pastoris. An efficient method based on bio-brick was developed to construct a series of vectors harboring different copy numbers of ROL gene cassettes, which were then transformed into P. pastoris GS115 to generate a strain with specific copy numbers of ROL. An optimized gene-dosage recombinant strain of GS115/pAOα-5ROL 11# harboring five copies of ROL was screened, revealing production of the highest activity (2700 U/mL), which was 8-fold higher than that of the strain harboring one copy...
February 13, 2018: Protein Expression and Purification
https://www.readbyqxmd.com/read/29451787/effect-of-methyl-substitution-on-the-n-h%C3%A2-%C3%A2-%C3%A2-o-interaction-in-complexes-of-pyrrole-with-water-methanol-and-dimethyl-ether-matrix-isolation-infrared-spectroscopy-and-ab-initio-computational-studies
#9
Shubhra Sarkar, Nagarajan Ramanathan, Kalyanasundaram Sundararajan
Hydrogen bonded interactions of pyrrole with water and methanol have been studied using matrix isolation infrared spectroscopy and compared with the calculation performed on dimethyl ether. Computations carried out at MP2/aug-cc-pVDZ level of theory yielded two minima for the pyrrole-water and pyrrole-methanol complexes. The global and local minima correspond to the N-H∙∙∙O and O-H∙∙∙π complexes, respectively, where the N-H group of pyrrole interacts with oxygen of water/methanol and O-H of water and methanol interacts with the π cloud of pyrrole...
February 16, 2018: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/29450982/application-of-off-line-two-dimensional-high-performance-countercurrent-chromatography-on-the-chloroform-soluble-extract-of-cuscuta-auralis-seeds
#10
Taewoong Rho, Kee Dong Yoon
In this study, the chloroform-soluble extract of C. auralis was separated successfully using off-line two-dimensional high-performance countercurrent chromatography, yielding a γ-pyrone, two alkaloids, a flavonoid and four lignans. The first-dimensional countercurrent separation using a methylene chloride/methanol/water (11:6:5, v/v/v) system yielded three sub-fractions (fractions I-III). The second-dimensional countercurrent separations, conducted on fractions I-III using n-hexane/ethyl and acetate/methanol/acetic acid (5:5:5:5:0, 3:7:3:7:0 and 1:9:1:9:0...
February 16, 2018: Journal of Separation Science
https://www.readbyqxmd.com/read/29449874/an-efficient-modified-method-for-plant-leaf-lipid-extraction-results-in-improved-recovery-of-phosphatidic-acid
#11
Sunitha Shiva, Regina Enninful, Mary R Roth, Pamela Tamura, Krishna Jagadish, Ruth Welti
Background: Lipidomics plays an important role in understanding plant adaptation to different stresses and improving our knowledge of the genes underlying lipid metabolism. Lipidomics involves lipid extraction, sample preparation, mass spectrometry analysis, and data interpretation. One of the practical challenges for large-scale lipidomics studies on plant leaves is the requirement of an efficient and rapid extraction method. Results: A single-extraction method with a polar solvent mixture gives results comparable to a widely used, multi-extraction method when tested on both Arabidopsis thaliana and Sorghum bicolor leaf tissue...
2018: Plant Methods
https://www.readbyqxmd.com/read/29449808/suppressive-effects-of-clerodendrum-volubile-p-beauv-labiatae-methanolic-extract-and-its-fractions-on-type-2-diabetes-and-its-complications
#12
Ochuko L Erukainure, Rahman M Hafizur, Nurul Kabir, M Iqbal Choudhary, Olubunmi Atolani, Priyanka Banerjee, Robert Preissner, Chika I Chukwuma, Aliyu Muhammad, Eric O Amonsou, Md Shahidul Islam
Type 2 diabetes is the most prominent of all diabetes types, contributing to global morbidity and mortality. Availability and cost of treatment with little or no side effect especially in developing countries, remains a huge burden. This has led to the search of affordable alternative therapies especially from medicinal plants. In this study, the antidiabetic effect of the methanolic extract, dichloromethane (DCM), butanol (BuOH) and aqueous fractions of Clerodendrum volubile leaves were investigated in type 2 diabetic rats for their effect on glucose homeostasis, serum insulin level and hepatic biomarkers, lipid profile, pancreatic redox balance and Ca 2+ levels, and β-cell distribution and function...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29449637/facile-emulsion-mediated-synthesis-of-phase-pure-diopside-nanoparticles
#13
Elena Tajuelo Rodriguez, Lawrence M Anovitz, Caleb D Clement, Adam J Rondinone, Michael C Cheshire
Diopside is a common natural pyroxene that is rarely found in a pure state, since magnesium is often partially substituted by iron, and other elements (sodium and aluminum) are often present. This pyroxene, along with feldspars and olivines, is common in concrete. As the prospective license renewal of light water reactors to 80 years of operation has raised concerns on the effects of radiation in the concrete biological shield surrounding the reactors, mineral nanoparticles can be valuable to perform amorphization studies to inform predictive models of mechanical properties of irradiated concrete...
February 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29449464/response-to-comment-on-selective-anaerobic-oxidation-of-methane-enables-direct-synthesis-of-methanol
#14
Vitaly L Sushkevich, Dennis Palagin, Marco Ranocchiari, Jeroen A van Bokhoven
Labinger argues that stepwise reaction of methane with water to produce methanol and hydrogen will never be commercially feasible because of its substoichiometric basis with respect to the active site and the requirement of a large temperature swing. This comment is not touching any new ground, beyond describing the thermodynamic feasibility, thermal cycling, and the role of water as discussed previously. Most important, it does not have a solid numerical basis.
February 16, 2018: Science
https://www.readbyqxmd.com/read/29449463/comment-on-selective-anaerobic-oxidation-of-methane-enables-direct-synthesis-of-methanol
#15
Jay A Labinger
The comment and response concerning the report of oxidation of methane to methanol by water (Reports, 5 May 2017, p. 523) do not fully capture the implications of thermodynamic limitations. A nonisothermal process in which each cycle requires a large temperature swing and permits only substoichiometric methane conversion surely could not be carried out on any practical scale.
February 16, 2018: Science
https://www.readbyqxmd.com/read/29449344/defeating-major-contaminants-in-fe-3-imac-phosphopeptide-enrichment
#16
Clement M Potel, Miao-Hsia Lin, Albert J R Heck, Simone Lemeer
Here we demonstrate that biomolecular contaminants, such as nucleic acid molecules, can seriously interfere with IMAC based phosphopeptide enrichments. We address and largely solve this issue, developing a robust protocol implementing methanol/chloroform protein precipitation and enzymatic digestion using benzonase, which degrades all forms of DNA and RNA, prior to IMAC-column loading. This simple procedure resulted in a drastic increase of enrichment sensitivity, enabling the identification of around 17,000 unique phosphopeptides and 12,500 unambiguously localized phosphosites in human cell-lines from a single LC-MS/MS run, constituting a 50% increase when compared to the standard protocol...
February 15, 2018: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/29449024/surrogate-alcohol-or-nonbeverage-alcohol-consumption-the-surrogate-alcohol-questionnaire-saq
#17
Ariela Green, Daniel Neff, Greg Giuliano, Nathan Lee, Rachel Turchin, Elisabeth J S Kunkel
BACKGROUND: Diagnosis and treatment of alcohol use disorders and their sequelae are common clinical questions for the consultation-liaison psychiatrist. At an urban, academic medical center, the authors consulted on several patients whose consumption of alcohol included nonbeverage forms of alcohol, (described in the literature as surrogate alcohols, nonbeverage alcohols, e.g., mouthwash). METHODS: The authors describe 4 patients who presented with surrogate alcohol consumption...
January 4, 2018: Psychosomatics
https://www.readbyqxmd.com/read/29448642/nanocomposite-membranes-comprising-crosslinked-polymer-blends-of-poly-vinyl-alcohol-poly-styrene-sulfonic-acid-co-maleic-acid-and-fumed-silica-nanoparticles
#18
Moon-Sung Kang, Yong Soo Kang, Hyoung-Juhn Kim, Hak-Soo Han, Jin-Soo Park
Nanocomposite polymer electrolyte membranes comprising a crosslinked polymer blend of poly(vinyl alcohol)/poly(styrene sulfonic acid-co-maleic acid) (PVA/PSSA-co-MA) and fumed silica nanoparticles were prepared for direct methanol fuel cell (DMFC) applications. Silica nanoparticles could be incorporated well uniformly in the completely miscible system, which can form a three-dimensional network structure to achieve the enhancement of mechanical properties as well as the additional reduction of methanol permeability...
March 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29448192/impact-of-enzymatic-hydrolysis-on-the-quantification-of-total-urinary-concentrations-of-chemical-biomarkers
#19
Prabha Dwivedi, Xiaoliu Zhou, Tolar G Powell, Antonia M Calafat, Xiaoyun Ye
Human exposure to consumer and personal care products chemicals such as phenols, including parabens and other antimicrobial agents, can be assessed through biomonitoring by quantifying urinary concentrations of the parent chemical or its metabolites, often after hydrolysis of phase II conjugates. Developing suitable analytical methods for the concurrent quantification of multiple exposure biomarkers is challenging because optimal conditions for the hydrolysis of such conjugates (e.g., O-glucuronides, N-glucuronides, sulfates) may differ depending on the biomarker...
February 12, 2018: Chemosphere
https://www.readbyqxmd.com/read/29447011/the-genotoxic-and-antigenotoxic-potential-of-the-methanolic-root-extract-of-glycyrrhiza-glabra-l-on-human-peripheral-blood-lymphocytes
#20
Ayşe Yavuz Kocaman, Müge Güzelkokar
Glycyrrhiza glabra L. (licorice) is one of the most important medicinal plants, which is widely used throughout the world both in traditional and contemporary medical industries. This study was undertaken to investigate the potential genotoxic activity of G. glabra methanolic root extract, and its possible antigenotoxic properties against mitomycin C (MMC)-induced DNA damage in in vitro chromosome aberrations (CAs) and cytokinesis-block micronucleus (CBMN) assays in human peripheral blood lymphocytes (PBLs)...
February 15, 2018: Drug and Chemical Toxicology
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