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https://www.readbyqxmd.com/read/28346372/-bligh-and-dyer-and-folch-methods-for-solid-liquid-liquid-extraction-of-lipids-from-microorganisms-comprehension-of-solvatation-mechanisms-and-towards-substitution-with-alternative-solvents
#1
Cassandra Breil, Maryline Abert Vian, Thomas Zemb, Werner Kunz, Farid Chemat
Bligh and Dyer (B & D) or Folch procedures for the extraction and separation of lipids from microorganisms and biological tissues using chloroform/methanol/water have been used tens of thousands of times and are "gold standards" for the analysis of extracted lipids. Based on the Conductor-like Screening MOdel for realistic Solvatation (COSMO-RS), we select ethanol and ethyl acetate as being potentially suitable for the substitution of methanol and chloroform. We confirm this by performing solid-liquid extraction of yeast (Yarrowia lipolytica IFP29) and subsequent liquid-liquid partition-the two steps of routine extraction...
March 27, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28345893/fluorescent-recognition-of-zn-2-by-two-diastereomeric-salicylaldimines-dramatically-different-responses-and-spectroscopic-investigation
#2
Ting Song, Yuan Cao, Gang Zhao, Lin Pu
Fluorescence responses of two BINOL-based diastereomeric salicylaldimines toward a variety of metal cations have been studied in methanol solution. It is revealed that both compounds show great fluorescence enhancements in the presence of Zn(2+) but not with any other metal ions. Moreover, these two diastereomers exhibit dramatically different responses toward Zn(2+) under the same conditions. That is, one can produce much stronger fluorescence enhancement also at a longer wavelength than the other. This fluorescence recognition of Zn(2+) also shows distinctive color changes under a UV lamp...
March 27, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28345446/inter-species-comparative-antioxidant-assay-and-hptlc-analysis-of-sakuranetin-in-the-chloroform-and-ethanol-extracts-of-aerial-parts-of-rhus-retinorrhoea-and-rhus-tripartita
#3
Perwez Alam, Mohammad Khalid Parvez, Ahmed Hassan Arbab, Nasir Ali Siddiqui, Mohammed Salem Al-Dosary, Adnan Jathlan Al-Rehaily, Sarfaraz Ahmed, Mohd Abul Kalam, Mohammad Shamim Ahmad
CONTEXT: Extensive research on Rhus (Anacardiaceae) shows their antioxidant potential, which warrants further evaluation of its other species. OBJECTIVE: To perform a comparative antioxidant assay on extracts of R. retinorrhoea and R. tripartita, including sakuranetin quantification by a validated HPTLC method. MATERIALS AND METHODS: In vitro antioxidant assay was performed on chloroform and ethanol extracts of R. retinorrhoea Steud. ex Oliv...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28345304/chemical-evidence-for-the-effect-of-urochloa-ruziziensis-on-glyphosate-resistant-soybeans
#4
Mariluce Nepomuceno, Nuria Chinchilla, Rosa M Varela, José M G Molinillo, Rodney Lacret, Pedro L C A Alves, Francisco A Macias
BACKGROUND: Soybean (Glycine max) is an important oleaginous legume that has been cultivated in new areas in Brazil, including pastures. Problems of reduced production yields have been reported by soybean growers when the crop is sown immediately after desiccation of pastures of Urochloa spp. using glyphosate. The objective of this work was to extract, isolate, and identify the major chemicals from U. ruziziensis that have phytotoxic activity and to evaluate the possible relation between this effect and the reduced soybean yield...
March 26, 2017: Pest Management Science
https://www.readbyqxmd.com/read/28344636/development-of-an-efficient-glucosinolate-extraction-method
#5
T Doheny-Adams, K Redeker, V Kittipol, I Bancroft, S E Hartley
BACKGROUND: Glucosinolates, anionic sulfur rich secondary metabolites, have been extensively studied because of their occurrence in the agriculturally important brassicaceae and their impact on human and animal health. There is also increasing interest in the biofumigant properties of toxic glucosinolate hydrolysis products as a method to control agricultural pests. Evaluating biofumigation potential requires rapid and accurate quantification of glucosinolates, but current commonly used methods of extraction prior to analysis involve a number of time consuming and hazardous steps; this study aimed to develop an improved method for glucosinolate extraction...
2017: Plant Methods
https://www.readbyqxmd.com/read/28344489/utilization-of-gel-electrophoreses-for-the-quantitative-estimation-of-digestive-enzyme-papain
#6
Magdy M Muharram, Maged S Abdel-Kader
SDS-PAGE densitometric method for analysis of papain in pharmaceutical formulations was developed and validated for the first time. Standard and samples were mixed with SDS sample buffer and denatured at 95 °C for 5 min and the gel was run at 20 mA and 200 V for 30-40 min in SDS-PAGE buffer. Gels were stained in Coomassie blue solution and distained by 5% methanol and 10% acetic acid. Destained gels were imaged and analyzed using the ChemiDoc™ XRS+ System. Bands of papain appeared at Rf value 0.78 ± 0...
March 2017: Saudi Pharmaceutical Journal: SPJ: the Official Publication of the Saudi Pharmaceutical Society
https://www.readbyqxmd.com/read/28344474/biologically-guided-isolation-of-leishmanicidal-secondary-metabolites-from-euphorbia-peplus-l
#7
Elham Amin, Abeer Moawad, Hossam Hassan
Leishmaniasis is a worldwide health problem, highly endemic in developing countries. Moreover, the severe side effects and the reported drug resistance make it an urgent need to search for effective drugs that can replace or supplement those currently used. In a research program designed to investigate the antileishmanial activity of plants collected from the Egyptian flora, twenty extracts from fifteen plants growing in Egypt have been investigated for in vitro leishmanicidal activity against Leishmania donovani promastigotes...
February 2017: Saudi Pharmaceutical Journal: SPJ: the Official Publication of the Saudi Pharmaceutical Society
https://www.readbyqxmd.com/read/28344406/attribution-of-antibacterial-and-antioxidant-activity-of-cassia-tora-extract-toward-its-growth-promoting-effect-in-broiler-birds
#8
Jyoti Sahu, K M Koley, B D Sahu
AIM: The study was conducted to evaluate the attribution of antibacterial and antioxidant activity of methanolic extract of Cassia tora toward its growth promoting effect in broiler birds. MATERIALS AND METHODS: A limit test was conducted for C. tora extract in Wistar albino rats. Phytochemical screening of methanolic extract of leaves of C. tora was carried out. In-vitro antibacterial activity was measured by disc diffusion method. 1-day-old Ven Cobb broiler birds (n=90) were randomly allocated into three groups consisting of three replicates with 10 birds in each group...
February 2017: Veterinary World
https://www.readbyqxmd.com/read/28344362/positive-serum-ethanol-concentration-on-admission-to-hospital-as-the-factor-predictive-of-treatment-outcome-in-acute-methanol-poisoning
#9
Sergey Zakharov, Olga Nurieva, Katerina Kotikova, Jaromir Belacek, Tomas Navratil, Daniela Pelclova
ABSTRACT: Mass methanol poisonings present a serious problem for health systems worldwide, with poor outcome associated with delayed treatment. Positive pre-hospital serum ethanol concentration may have predictive value as the prognostic factor of the treatment outcome. We studied the effect of positive serum ethanol level on admission to hospital on survival in patients treated during the Czech methanol outbreak during 2012-2014. Cross-sectional cohort study was performed in 100 hospitalized patients with confirmed methanol poisoning...
2017: Monatshefte Für Chemie
https://www.readbyqxmd.com/read/28344084/a-rapid-procedure-for-the-in-situ-assay-of-periplasmic-pqq-dependent-methanol-dehydrogenase-in-intact-single-bacterial-colonies
#10
Venkata Ramana Vemuluri, Shreya Shaw, Caroline Autenrieth, Robin Ghosh
Mechanistic details of methanol oxidation catalyzed by the periplasmically-located pyrroloquinoline quinone-dependent methanol dehydrogenase of methylotrophs can be elucidated using site-directed mutants. Here, we present an in situ colony assay of methanol dehydrogenase, which allows robotic screening of large populations of intact small colonies, and regrowth of colonies for subsequent analysis.
March 23, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28343347/bioanalysis-of-farnesyl-pyrophosphate-in-human-plasma-by-high-performance-liquid-chromatography-coupled-to-triple-quadrupole-tandem-mass-spectrometry-and-hybrid-quadrupole-orbitrap-high-resolution-mass-spectrometry
#11
Hiroshi Sugimoto, Mie Iguchi, Fumihiro Jinno
The isoprenoids farnesyl pyrophosphate (FPP) and geranylgeranyl pyrophosphate (GGPP) are pivotal intermediates for cholesterol homeostasis and cell signaling in the mevalonate pathway. We developed a sensitive and selective high-performance liquid chromatography tandem triple quadrupole mass spectrometry (LC-QQQ-MS) method for FPP in human plasma without the need for a derivatization process. We optimized the sample preparation procedure to extract FPP and (13)C5-FPP (as internal standard) from sample fluids using methanol...
March 25, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28343077/separation-and-characterization-of-unknown-impurities-and-isomers-in-flomoxef-sodium-by-lc-it-tof-ms-and-study-of-their-negative-ion-fragmentation-regularities
#12
Xu Yu, Fan Wang, Jiani Li, Weiguang Shan, Bingqi Zhu, Jian Wang
Thirteen unknown impurities in flomoxef sodium were separated and characterized by liquid chromatography coupled with high resolution ion trap/time-of-flight mass spectrometry (LC-IT-TOF MS)with positive and negative modes of electrospray ionization method for further improvement of official monographs in pharmacopoeias. The fragmentation patterns of impurities in flomoxef in the negative ion mode were studied in detail, and new negative-ion fragmentation regularities were discovered. Chromatographic separation was performed on a Kromasil C18 column (250mm×4...
March 19, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28343034/bioactivities-of-achillea-phrygia-and-bupleurum-croceum-based-on-the-composition-of-phenolic-compounds-in-vitro-and-in-silico-approaches
#13
Gokhan Zengin, Gizem Bulut, Adriano Mollica, Mehmet Zeki Haznedaroglu, Ahmet Dogan, Abdurrahman Aktumsek
This study presents the effects of the Achillea phrygia Boiss. et Bal. and Bupleurum croceum Fenzl. extracts obtained by different solvents (ethyl acetate, methanol and water) on selected enzyme inhibitory effects and antioxidant ability with screening bioactive compounds. Total and individual bioactive compounds were detected by spectrophotometric and HPLC-DAD techniques. Antioxidant abilities were evaluated by different methods including free radical scavenging (ABTS and DPPH), reducing power (CUPRAC and FRAP), phosphomolybdenum and metal chelating...
March 22, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28342857/antinociceptive-and-anti-inflammatory-effects-of-the-methanolic-stem-bark-extract-of-antrocaryon-klaineanum-pierre-anacardiaceae-in-mice-and-rats
#14
Annie Laure Magne Fongang, Edwige Laure Nguemfo, Yolande Djouatsa Nangue, Calvin Bogning Zangueu, Yannick Fouokeng, Anatole Guy Blaise Azebaze, Eulogio José Llorent-Martínez, Maria Luisa Fernández-de Córdova, Alain Bertrand Dongmo, Wolfgang Vierling
ETHNOPHARMACOLOGICAL RELEVANCE: Antrocaryon klaineanum is used by traditional healers to treat many disorders including pain and inflammatory diseases. This study aimed to evaluate the analgesic and antiinflammatory activities of methanol extract of A. klaineanum in mice and rats. MATERIALS AND METHODS: Reverse phase high-performance liquid chromatography (RP-HPLC) was performed to establish the chromatographic fingerprint and to identify various chemical components of the plant extract...
March 22, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28342369/sensing-properties-of-monolayer-borophane-nanosheet-towards-alcohol-vapors-a-first-principles-study
#15
V Nagarajan, R Chandiramouli
The electronic properties of borophane nanosheet and adsorption behavior of three distinct alcohol vapors namely methanol, ethanol and 1-propanol on borophane nanosheet is studied using density functional theory method for the first time. The state-of-the-art provides insights on to the development of new two dimensional materials with the surface passivation on boron nanostructures. The density of states spectrum provides a clear perception on charge transfer upon adsorption of alcohol vapors on borophane nanosheets...
February 8, 2017: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/28342292/ultra-fast-liquid-chromatography-with-tandem-mass-spectrometry-determination-of-eight-bioactive-components-of-kai-xin-san-in-rat-plasma-and-its-application-to-a-comparative-pharmacokinetic-study-in-normal-and-alzheimer-s-disease-rats
#16
Xiaotong Wang, Yue Zhang, Huibin Niu, Yajing Geng, Bing Wang, Xiaomei Yang, Pengyu Yan, Qing Li, Kaishun Bi
A method of ultra-fast liquid chromatography with tandem mass spectrometry was developed and validated for the simultaneous quantitation of eight bioactive components, including polygalaxanthone III, sibiricaxanthone B, tenuifolin, sibiricose A5, sibiricose A6, tenuifoliside A, ginsenoside Re and ginsenoside Rb1 in rat plasma after oral administration of Kai-Xin-San. The plasma samples were extracted by liquid-liquid extraction using digoxin as internal standard. Chromatographic separation was performed on a Venusil MP C18 column (100 mm × 2...
March 25, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28342171/expressing-anti-hiv-vrc01-antibody-using-the-murine-igg1-secretion-signal-in-pichia-pastoris
#17
Rochelle Aw, Paul F McKay, Robin J Shattock, Karen M Polizzi
The use of the recombinant expression platform Pichia pastoris to produce pharmaceutically important proteins has been investigated over the past 30 years. Compared to mammalian cultures, expression in P. pastoris is cheaper and faster, potentially leading to decreased costs and process development times. Product yields depend on a number of factors including the secretion signal chosen for expression, which can influence the host cell response to recombinant protein production. VRC01, a broadly neutralising anti-HIV antibody, was expressed in P...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28342023/biogenic-synthesis-of-marsilea-quadrifolia-gold-nanoparticles-a-study-of-improved-glucose-utilization-efficiency-on-3t3-l1-adipocytes
#18
Anindita Chowdhury, Selvaraj Kunjiappan, Chiranjib Bhattacharjee, Balasubramanian Somasundaram, Theivendren Panneerselvam
This study aims mainly to provide an insight and understanding of the effect of glucose utilization efficiency of biogenic gold nanoparticles (GNPs) synthesized through the mediation of Marsilea quadrifolia (M. quadrifolia) methanol extract on 3T3-L1 adipocytes. The biosynthesized GNPs were characterized by UV visible spectrophotometry and FTIR. Simultaneously, the nature, stability, and morphological characteristics were analyzed by XRD, TG-DTA, SEM-EDS, HRTEM, and SAED. The results of characterization studies were used to assess the properties of GNPs...
March 24, 2017: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/28341989/a-fully-validated-bioanalytical-method-using-an-uhplc-ms-ms-system-for-quantification-of-dna-and-rna-oxidative-stress-biomarkers
#19
Barbora Cervinkova, Lenka Kujovska Krcmova, Veronika Sestakova, Dagmar Solichova, Petr Solich
A new, rapid and effective ultra-high-performance liquid chromatography method with mass spectrometry detection is described for the separation and quantification of 8-hydroxy-2-deoxyguanosine, 8-hydroxyguanosine and creatinine in human urine. The present study uses an isotope-labelled internal standard ([(15)N]5-8-hydroxy-2-deoxyguanosine), a BIO core-shell stationary phase and an isocratic elution of methanol and water. Sample preparation of human urine was performed by solid-phase extraction (SPE) on Oasis HLB cartridges with methanol/water 50:50 (v/v) elution...
March 24, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28341432/development-validation-and-evaluation-of-an-analytical-method-for-the-determination-of-monomeric-and-oligomeric-procyanidins-in-apple-extracts
#20
Wendy J Hollands, Stefan Voorspoels, Griet Jacobs, Kjersti Aaby, Ane Meisland, Rocio Garcia-Villalba, Francisco Tomas-Barberan, Mariusz K Piskula, Deborah Mawson, Irena Vovk, Paul W Needs, Paul A Kroon
There is a lack of data for individual oligomeric procyanidins in apples and apple extracts. Our aim was to develop, validate and evaluate an analytical method for the separation, identification and quantification of monomeric and oligomeric flavanols in apple extracts. To achieve this, we prepared two types of flavanol extracts from freeze-dried apples; one was an epicatechin-rich extract containing ∼30% (w/w) monomeric (-)-epicatechin which also contained oligomeric procyanidins (Extract A), the second was an oligomeric procyanidin-rich extract depleted of epicatechin (Extract B)...
March 18, 2017: Journal of Chromatography. A
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