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https://www.readbyqxmd.com/read/28248852/serosurveillance-of-hepatitis-a-in-a-region-which-adopted-the-universal-mass-vaccination
#1
Maria Filomena Gallone, Francesco Desiante, Maria Serena Gallone, Giovanna Barbuti, Silvio Tafuri, Cinzia Germinario
Hepatitis A is a common infectious disease worldwide that was endemic in many regions of Southern Italy, such as Apulia region. After a large hepatitis A outbreak occurred between 1996 and 1997, in Apulia an active-free immunization program that was targeted to new-borns and adolescents was started. The aim of this study is to investigate the hepatitis A seroprevalence in the adult Apulian population 18 years after the immunization program introduction, in order to evaluate the risk of new epidemics onset.The study was carried out from May 2011 to June 2012 among blood donors from Department of Transfusion Medicine and Blood Bank of Policlinico General Hospital in Bari...
March 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28182231/a-gain-of-function-mutation-in-the-cardiac-pacemaker-hcn4-channel-increasing-camp-sensitivity-is-associated-with-familial-inappropriate-sinus-tachycardia
#2
Mirko Baruscotti, Annalisa Bucchi, Raffaella Milanesi, Manuel Paina, Andrea Barbuti, Tomaso Gnecchi-Ruscone, Elisabetta Bianco, Laura Vitali-Serdoz, Riccardo Cappato, Dario DiFrancesco
No abstract text is available yet for this article.
January 21, 2017: European Heart Journal
https://www.readbyqxmd.com/read/28131876/modulating-the-function-of-atp-binding-cassette-subfamily-g-member-2-abcg2-with-inhibitor-cabozantinib
#3
Guan-Nan Zhang, Yun-Kai Zhang, Yi-Jun Wang, Anna Maria Barbuti, Xi-Jun Zhu, Xin-Yue Yu, Ai-Wen Wen, John N D Wurpel, Zhe-Sheng Chen
Cabozantinib (XL184) is a small molecule tyrosine kinase receptor inhibitor, which targets c-Met and VEGFR2. Cabozantinib has been approved by the Food and Drug Administration to treat advanced medullary thyroid cancer and renal cell carcinoma. In the present study, we evaluated the ability of cabozantinib to modulate the function of the ATP-binding cassette subfamily G member 2 (ABCG2) by sensitizing cells that are resistant to ABCG2 substrate antineoplastic drugs. We used a drug-selected resistant cell line H460/MX20 and three ABCG2 stable transfected cell lines ABCG2-482-R2, ABCG2-482-G2, and ABCG2-482-T7, which overexpress ABCG2...
May 2017: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/27918948/adult-autism-subthreshold-spectrum-adas-spectrum-validation-of-a-questionnaire-investigating-subthreshold-autism-spectrum
#4
L Dell'Osso, C Gesi, E Massimetti, I M Cremone, M Barbuti, G Maccariello, I Moroni, S Barlati, G Castellini, M Luciano, L Bossini, M Rocchetti, M Signorelli, E Aguglia, A Fagiolini, P Politi, V Ricca, A Vita, C Carmassi, M Maj
AIM: Increasing literature has shown the usefulness of a dimensional approach to autism. The present study aimed to determine the psychometric properties of the Adult Autism Subthreshold Spectrum (AdAS Spectrum), a new questionnaire specifically tailored to assess subthreshold forms of autism spectrum disorder (ASD) in adulthood. METHODS: 102 adults endorsing at least one DSM-5 symptom criterion for ASD (ASDc), 143 adults diagnosed with a feeding and eating disorder (FED), and 160 subjects with no mental disorders (CTL), were recruited from 7 Italian University Departments of Psychiatry and administered the following: SCID-5, Autism-Spectrum Quotient (AQ), Ritvo Autism and Asperger Diagnostic Scale 14-item version (RAADS-14), and AdAS Spectrum...
February 2017: Comprehensive Psychiatry
https://www.readbyqxmd.com/read/27800441/study-on-potential-clostridium-botulinum-growth-and-toxin-production-in-parma-ham
#5
Giuseppe Merialdi, Mattia Ramini, Giovanni Parolari, Silvana Barbuti, Maria Angela Frustoli, Roberta Taddei, Stefano Pongolini, Paolo Ardigò, Paolo Cozzolino
The objective of this study was to investigate Clostridium botulinum growth and toxin production in the industrially manufactured Italian Parma ham. The study focuses on the Parma ham production phase identified as maximum risk to C. botulinum proliferation, i.e. the transition from cold phase (salting and resting) to a phase carried out at temperature between 15 and 23°C (drying). A preliminary in vitro test was carried out in order to verify the capability of 6 C. botulinum strains (1 type A, 4 type B, and 1 type E strains) to grow in conditions of temperature, pH and NaCl concentration comparable to those of the beginning stage of ham drying...
April 19, 2016: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/27783346/microbiological-and-hydrogeological-assessment-of-groundwater-in-southern-italy
#6
Osvalda De Giglio, Giovanna Barbuti, Paolo Trerotoli, Silvia Brigida, Angelantonio Calabrese, Giuseppe Di Vittorio, Grazia Lovero, Giuseppina Caggiano, Vito Felice Uricchio, Maria Teresa Montagna
This study represents the first investigation of microbiological groundwater pollution as a function of aquifer type and season for the Apulia region of southern Italy. Two hundred and seven wells were randomly selected from those monitored by the Regional Agency for Environmental Protection for emergency use. Both compulsory (Escherichia coli, Total Coliform, and Enterococci) and optional (Pseudomonas aeruginosa, Salmonella spp., Heterotrophic Plate Count at 37 and 22 °C) microbiological parameters were assessed regularly at these wells...
November 2016: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/27599668/human-derived-cardiomyocytes-a-decade-of-knowledge-after-the-discovery-of-induced-pluripotent-stem-cells
#7
REVIEW
Andrea Barbuti, Patrizia Benzoni, Giulia Campostrini, Patrizia Dell'Era
Ten years ago Yamanaka's lab identified a way to reprogram terminally differentiated cells to a pluripotent state, similar to that of embryonic stem cell. This procedure opened the road for the generation of postmitotic human cells, that have completely lost the replication potential. The initial excitement waned when it was observed that the cells produced by this method are somehow immature and do not resemble the adult phenotype. In the absence of cellular markers that recognize the various maturation steps of induced pluripotent stem cell-derived human cardiomyocytes, we propose to follow their maturation looking at their electrophysiological profile...
December 2016: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/27422791/bone-turnover-response-is-linked-to-both-acute-and-established-metabolic-changes-in-ultra-marathon-runners
#8
Veronica Sansoni, Gianluca Vernillo, Silvia Perego, Andrea Barbuti, Giampiero Merati, Federico Schena, Antonio La Torre, Giuseppe Banfi, Giovanni Lombardi
Bone and energy metabolisms regulation depends on a two-way street aimed at regulating energy utilization. Mountain ultra-marathons are highly demanding aerobic performances that deeply affect the whole body homeostasis. In this study we aimed to investigate and characterize the metabolic profile (in terms of hormones involved in energy metabolism), the inflammatory adipokines, and the bone turnover; in particular the osteocalcin-mediated response has been compared in experienced mountain ultra-marathons runners versus control subjects...
July 15, 2016: Endocrine
https://www.readbyqxmd.com/read/27276530/plasminogen-activator-inhibitor-1-as-a-marker-of-cardiovascular-response-in-professional-mountain-ultra-marathon-runners
#9
LETTER
Giovanni Lombardi, Gianluca Vernillo, Veronica Sansoni, Silvia Perego, Andrea Barbuti, Giampiero Merati, Federico Schena, Giuseppe Banfi, Antonio La Torre
No abstract text is available yet for this article.
January 1, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/26985660/localizing-tortoise-nests-by-neural-networks
#10
Roberto Barbuti, Stefano Chessa, Alessio Micheli, Rita Pucci
The goal of this research is to recognize the nest digging activity of tortoises using a device mounted atop the tortoise carapace. The device classifies tortoise movements in order to discriminate between nest digging, and non-digging activity (specifically walking and eating). Accelerometer data was collected from devices attached to the carapace of a number of tortoises during their two-month nesting period. Our system uses an accelerometer and an activity recognition system (ARS) which is modularly structured using an artificial neural network and an output filter...
2016: PloS One
https://www.readbyqxmd.com/read/26980505/prevalence-and-characterization-of-methicillin-resistant-staphylococcus-aureus-isolates-in-food-industry-workers
#11
G Caggiano, A Dambrosio, F Ioanna, S Balbino, G Barbuti, O De Giglio, G Diella, G Lovero, S Rutigliano, G Scarafile, A Baldassarre, L Vimercati, M Musti, M T Montagna
BACKGROUND: Methicillin-resistant S. aureus (MRSA) is a pathogen spread not only in the hospital environment but also in the community and amongst livestock (LA-MRSA). LA-MRSA can be transmitted to humans that live in close contact with MRSA-colonized animals, and human colonization and/or infection has been reported worldwide, particularly among those involved with livestock farming. In this study the authors evaluated the prevalence of S. aureus and MRSA among healthy carriers who worked in the food industry in Apulia, Southern Italy...
January 2016: Annali di Igiene: Medicina Preventiva e di Comunità
https://www.readbyqxmd.com/read/26709802/higher-cardiogenic-potential-of-ipscs-derived-from-cardiac-versus-skin-stromal-cells
#12
Viviana Meraviglia, Jianyan Wen, Luca Piacentini, Giulia Campostrini, Cheng Wang, Maria Cristina Florio, Valerio Azzimato, Lorenzo Fassina, Martin Langes, Johnson Wong, Michele Miragoli, Carlo Gaetano, Giulio Pompilio, Andrea Barbuti, Dario DiFrancesco, Deborah Mascalzoni, Peter P Pramstaller, Gualtiero I Colombo, Huei-Sheng Vincent Chen, Alessandra Rossini
Prior studies have demonstrated that founder cell type could influence induced pluripotent stem cells (iPSCs) molecular and developmental properties at early passages after establishing their pluripotent state. Herein, we evaluated the persistence of a functional memory related to the tissue of origin in iPSCs from syngeneic cardiac (CStC) vs skin stromal cells (SStCs). We found that, at passages greater than 15, iPSCs from cardiac stromal cells (C-iPSCs) produced a higher number of beating embryoid bodies than iPSCs from skin stromal cells (S-iPSCs)...
January 1, 2016: Frontiers in Bioscience (Landmark Edition)
https://www.readbyqxmd.com/read/26633515/paclitaxel-through-the-ages-of-anticancer-therapy-exploring-its-role-in-chemoresistance-and-radiation-therapy
#13
REVIEW
Anna Maria Barbuti, Zhe-Sheng Chen
Paclitaxel (Taxol(®)) is a member of the taxane class of anticancer drugs and one of the most common chemotherapeutic agents used against many forms of cancer. Paclitaxel is a microtubule-stabilizer that selectively arrests cells in the G2/M phase of the cell cycle, and found to induce cytotoxicity in a time and concentration-dependent manner. Paclitaxel has been embedded in novel drug formulations, including albumin and polymeric micelle nanoparticles, and applied to many anticancer treatment regimens due to its mechanism of action and radiation sensitizing effects...
2015: Cancers
https://www.readbyqxmd.com/read/26553542/a-gain-of-function-mutation-in-the-cardiac-pacemaker-hcn4-channel-increasing-camp-sensitivity-is-associated-with-familial-inappropriate-sinus-tachycardia
#14
Mirko Baruscotti, Annalisa Bucchi, Raffaella Milanesi, Manuel Paina, Andrea Barbuti, Tomaso Gnecchi-Ruscone, Elisabetta Bianco, Laura Vitali-Serdoz, Riccardo Cappato, Dario DiFrancesco
AIMS: Inappropriate Sinus Tachycardia (IST), a syndrome characterized by abnormally fast sinus rates and multisystem symptoms, is still poorly understood. Because of the relevance of HCN4 channels to pacemaker activity, we used a candidate-gene approach and screened IST patients for the presence of disease-causing HCN4 mutations. METHODS AND RESULTS: Forty-eight IST patients, four of whom of known familial history, were enrolled in the study. We initially identified in one of the patients with familial history the R524Q mutation in HCN4...
November 9, 2015: European Heart Journal
https://www.readbyqxmd.com/read/26551156/mathematical-modeling-of-drug-resistance-due-to-kras-mutation-in-colorectal-cancer
#15
Sheema Sameen, Roberto Barbuti, Paolo Milazzo, Antonio Cerone, Marzia Del Re, Romano Danesi
The most challenging task in colorectal cancer research nowadays is to understand the development of acquired resistance to anti-EGFR drugs. The key reason for this problem is the KRAS mutations appearance after the treatment with monoclonal antibodies (moAb). Here we present a mathematical model for the analysis of KRAS mutations behavior in colorectal cancer with respect to moAb treatments. To evaluate the drug performance we have developed equations for two types of tumors cells, KRAS mutated and KRAS wild-type...
January 21, 2016: Journal of Theoretical Biology
https://www.readbyqxmd.com/read/26547215/anacardic-acid-and-thyroid-hormone-enhance-cardiomyocytes-production-from-undifferentiated-mouse-es-cells-along-functionally-distinct-pathways
#16
Agnese Re, Simona Nanni, Aurora Aiello, Serena Granata, Claudia Colussi, Giulia Campostrini, Francesco Spallotta, Stefania Mattiussi, Valentina Pantisano, Carmen D'Angelo, Annamaria Biroccio, Alessandra Rossini, Andrea Barbuti, Dario DiFrancesco, Francesco Trimarchi, Alfredo Pontecorvi, Carlo Gaetano, Antonella Farsetti
The epigenetics of early commitment to embryonal cardiomyocyte is poorly understood. In this work, we compared the effect of thyroid hormone and that of anacardic acid, a naturally occurring histone acetylase inhibitor, or both in combination, on mouse embryonic stem cells (mES) differentiating into embryonal cardiomyocyte by embryoid bodies (EBs) formation. Although the results indicated that anacardic acid (AA) and thyroid hormone were both efficient in promoting cardiomyocyte differentiation, we noticed that a transient exposure of mES to AA alone was sufficient to enlarge the beating areas of EBs compared to those of untreated controls...
September 2016: Endocrine
https://www.readbyqxmd.com/read/26335050/correlation-between-manchester-grading-scale-and-american-orthopaedic-foot-and-ankle-society-score-in-patients-with-hallux-valgus
#17
Kalliopi Iliou, George Paraskevas, Panagiotis Kanavaros, Alexandra Barbouti, Aristidis Vrettakos, Christos Gekas, Panagiotis Kitsoulis
OBJECTIVE: To evaluate the correlation between the Manchester Grading Scale and the American Orthopaedic Foot and Ankle Society (AOFAS) score in patients with a hallux valgus deformity. SUBJECTS AND METHODS: The study sample included 181 feet of 122 patients with hallux valgus and 424 feet of 212 individuals without hallux valgus deformity as the control group. The severity of hallux valgus, utilizing a relative nonmetric scale, the Manchester Grading Scale, and the metric AOFAS score, was determined for all individuals in the hallux valgus and control groups...
2016: Medical Principles and Practice: International Journal of the Kuwait University, Health Science Centre
https://www.readbyqxmd.com/read/26264759/acetylation-mediates-cx43-reduction-caused-by-electrical-stimulation
#18
Viviana Meraviglia, Valerio Azzimato, Claudia Colussi, Maria Cristina Florio, Anna Binda, Alice Panariti, Khaled Qanud, Silvia Suffredini, Laura Gennaccaro, Michele Miragoli, Andrea Barbuti, Paul D Lampe, Carlo Gaetano, Peter P Pramstaller, Maurizio C Capogrossi, Fabio A Recchia, Giulio Pompilio, Ilaria Rivolta, Alessandra Rossini
Communication between cardiomyocytes depends upon gap junctions (GJ). Previous studies have demonstrated that electrical stimulation induces GJ remodeling and modifies histone acetylase (HAT) and deacetylase (HDAC) activities, although these two results have not been linked. The aim of this work was to establish whether electrical stimulation modulates GJ-mediated cardiac cell-cell communication by acetylation-dependent mechanisms. Field stimulation of HL-1 cardiomyocytes at 0.5 Hz for 24 h significantly reduced connexin43 (Cx43) expression and cell-cell communication...
October 2015: Journal of Molecular and Cellular Cardiology
https://www.readbyqxmd.com/read/26215172/postexercise-autonomic-function-after-repeated-sprints-training
#19
RANDOMIZED CONTROLLED TRIAL
Gianluca Vernillo, Luca Agnello, Andrea Barbuti, Silvia Di Meco, Giovanni Lombardi, Giampiero Merati, Antonio La Torre
PURPOSE: We examined the effects of an 8-week repeated-sprint (RS) training protocol on postexercise parasympathetic reactivation (PNSr) in healthy adults. METHODS: Eighteen male adults (24.3 ± 3.7 years) were assigned to either of two groups. One group (n = 9) performed RS training (EXP, 3 times week(-1), 18 maximal all-out 15-m sprints interspersed with 17 s of passive recovery); the other served as the control group (CON, n = 9). Performance before, during, and after was assessed by measuring RS ability time (S dec) and total sprint time...
November 2015: European Journal of Applied Physiology
https://www.readbyqxmd.com/read/26064098/standard-triple-therapy-versus-sequential-therapy-in-helicobacter-pylori-eradication-a-double-blind-randomized-and-controlled-trial
#20
Jaime Natan Eisig, Tomás Navarro-Rodriguez, Ana Cristina Sá Teixeira, Fernando Marcuz Silva, Rejane Mattar, Decio Chinzon, Christiane Haro, Márcio Augusto Diniz, Joaquim Prado Moraes-Filho, Ronnie Fass, Ricardo Correa Barbuti
Aim. To compare 10-day standard triple therapy versus sequential therapy as first-line treatment in patients infected with H. pylori. Methods. One hundred H. pylori positive patients (diagnosed by rapid urease test and histology), with average age of 47.2, M/F = 28/72, were randomized to receive either standard triple treatment (TT) as follows: lansoprazole 30 mg, clarithromycin 500 mg, and amoxicillin 1 g, b.i.d. for ten days, or sequential treatment (ST) as follows: lansoprazole 30 mg, amoxicillin and placebo 1...
2015: Gastroenterology Research and Practice
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