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Minimally invasive preparation

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https://www.readbyqxmd.com/read/29025646/nanographene-oxide-as-a-switch-for-cw-pulsed-nir-laser-triggered-drug-release-from-liposomes
#1
Abhishek Sahu, Manse Kim, Jooyeon Ryu, Joon-Gon Son, Eunji Lee, Do Young Noh, Giyoong Tae
The application of pulsed and continuous wave (CW) lasers in nanomedicine has increased significantly over the last decade. Near infrared (NIR) lasers can be used for the precise control of drug release at the target site in a non-invasive manner. In this study, we have prepared nanographene oxide (nGO, size ~40nm) integrated liposomes (size ~900nm). The nGOs were not simply adsorbed onto the liposome surface but was embedded inside the liposomes as characterized by cryo-TEM, selected area electron diffraction (SAED), and fluorescence quenching studies...
January 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28994784/technique-of-minimally-invasive-transverse-aortic-constriction-in-mice-for-induction-of-left-ventricular-hypertrophy
#2
Reza Tavakoli, Simona Nemska, Peiman Jamshidi, Max Gassmann, Nelly Frossard
Transverse aortic constriction (TAC) in mice is one of the most commonly used surgical techniques for experimental investigation of pressure overload-induced left ventricular hypertrophy (LVH) and its progression to heart failure. In the majority of the reported investigations, this procedure is performed with intubation and ventilation of the animal which renders it demanding and time-consuming and adds to the surgical burden to the animal. The aim of this protocol is to describe a simplified technique of minimally invasive TAC without intubation and ventilation of mice...
September 25, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28994030/gelc-ms-a-sample-preparation-method-for-proteomics-analysis-of-minimal-amount-of-tissue
#3
Manousos Makridakis, Antonia Vlahou
Application of various proteomics methodologies have been implemented for the global and targeted proteome analysis of many different types of biological samples such as tissue, urine, plasma, serum, blood, and cell lines. Among the aforementioned biological samples, tissue has an exceptional role into clinical research and practice. Disease initiation and progression is usually located at the tissue level of different organs, making the analysis of this material very important for the understanding of the disease pathophysiology...
October 10, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28993303/the-lived-experience-of-being-evaluated-for-organ-donation-focus-groups-with-living-kidney-donors
#4
Camilla S Hanson, Angelique F Ralph, Karine E Manera, John S Gill, John Kanellis, Germaine Wong, Jonathan C Craig, Jeremy R Chapman, Allison Tong
BACKGROUND AND OBJECTIVES: Comprehensive evaluations are required to safeguard voluntarism and minimize harm to living kidney donors. This process is lengthy, invasive, and emotionally challenging, with up to one fifth of potential donors opting out. We aimed to describe donors' experiences of the evaluation process. DESIGN, SETTING, PARTICIPANTS, & MEASUREMENTS: We conducted 14 focus groups involving 123 kidney donors who completed donation from three transplant centers (Australia and Canada)...
October 9, 2017: Clinical Journal of the American Society of Nephrology: CJASN
https://www.readbyqxmd.com/read/28993084/perioperative-fractures-in-cementless-total-hip-arthroplasty-using-the-direct-anterior-minimally-invasive-approach-reduced-risk-with-short-stems
#5
Michael Dietrich, Method Kabelitz, Claudio Dora, Patrick O Zingg
BACKGROUND: The direct minimally invasive anterior approach (DMIAA) and the use of uncemented stems demonstrated an increase in intraoperative fractures in recent literature. Whether the different design of the stems additionally influences the incidence of perioperative local complications, was the goal of this study. METHODS: From January 2008 until June 2010, all patients undergoing primary cementless total hip arthroplasty, using a DMIAA, were consecutively included...
September 19, 2017: Journal of Arthroplasty
https://www.readbyqxmd.com/read/28987833/training-of-intra-axial-brain-tumor-resection-using-a-self-made-simple-device-with-agar-and-gelatin
#6
Toshihiro Mashiko, Hirohumi Oguma, Takehiko Konno, Akira Gomi, Takashi Yamaguchi, Rie Nagayama, Makoto Sato, Ryo Iwase, Kensuke Kawai
Self-made devices composed of agar and gelatin gel have been used for resident training of intra-axial brain tumor resection. The mixture gel of agar and gelatin is retractable and can be suctioned. Hardness of the gel depends on the concentration of the solution. Therefore, by changing the concentration, it is easy to make gels of various hardness. In this study, a mass of gel that looked like a tumor was put into another gel that looked like the brain. A part of the "brain" was regarded as the eloquent area...
October 4, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/28983530/ceramic-laminate-veneers-clinical-procedures-with-a-multidisciplinary-approach
#7
Marco Veneziani
Complex cases with high esthetic needs represent a challenge for clinicians. A multidisciplinary approach is vital to achieve the planned result. New technological devices are needed to facilitate the collaboration between the clinical team members and to develop a fluent and effective diagnostic and therapeutic pathway. This article describes a well-defined protocol for the treatment of complex esthetic cases with the use of ceramic laminate veneers. The protocol involves different branches of dentistry: periodontal therapy, mucogingival surgery, restorative dentistry, orthodontics, and prosthodontics...
2017: International Journal of Esthetic Dentistry
https://www.readbyqxmd.com/read/28972736/design-and-functionalization-of-the-nir-responsive-photothermal-semiconductor-nanomaterials-for-cancer-theranostics
#8
Xiaojuan Huang, Wenlong Zhang, Guoqiang Guan, Guosheng Song, Rujia Zou, Junqing Hu
Despite the development of medical technology, cancer still remains a great threat to the survival of people all over the world. Photothermal therapy (PTT) is a minimally invasive method for selective photothermal ablation of cancer cells without damages to normal cells. Recently, copper chalcogenide semiconductors have emerged as a promising photothermal agent attributed to strong absorbance in the near-infrared (NIR) region and high photothermal conversion efficiency. An earlier study witnessed a rapid increase in their development for cancer therapy, including CuS, Cu2-xSe and CuTe nanocrystals...
October 3, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28966833/successful-secure-high-definition-streaming-telecytology-for-remote-cytologic-evaluation
#9
Sahussapont Joseph Sirintrapun, Dorota Rudomina, Allix Mazzella, Rusmir Feratovic, Oscar Lin
BACKGROUND: The use of minimally invasive procedures to obtain material for diagnostic purposes has become more prevalent in recent years. As such, there is increased demand for immediate cytologic adequacy assessment of minimally invasive procedures. The array of different locations in which rapid on-site evaluation (ROSE) is expected requires an ever-increasing number of cytology personnel to provide support for adequacy assessment. In our study, we describe the implementation process of a telecytology (TC) system in a high case volume setting and evaluate the performance of this activity...
2017: Journal of Pathology Informatics
https://www.readbyqxmd.com/read/28956933/injectable-anisotropic-nanocomposite-hydrogels-direct-in-situ-growth-and-alignment-of-myotubes
#10
Kevin J De France, Kevin G Yager, Katelyn J W Chan, Brandon Corbett, Emily D Cranston, Todd Hoare
While injectable in situ cross-linking hydrogels have attracted increasing attention as minimally invasive tissue scaffolds and controlled delivery systems, their inherently disorganized and isotropic network structure limits their utility in engineering oriented biological tissues. Traditional methods to prepare anisotropic hydrogels are not easily translatable to injectable systems given the need for external equipment to direct anisotropic gel fabrication and/or the required use of temperatures or solvents incompatible with biological systems...
October 11, 2017: Nano Letters
https://www.readbyqxmd.com/read/28950194/one-pot-synthesis-of-aie-based-bismuth-sulfide-nanotheranostics-for-fluorescence-imaging-and-photothermal-therapy
#11
Ke Wang, Jialang Zhuang, Liang Chen, Dazhuang Xu, Xiaoyong Zhang, Zuanguang Chen, Yen Wei, Yuanqing Zhang
Photothermal therapy (PTT) has been widely investigated as a minimally invasive strategy for cancer therapy due to its favorable biosafety. Nevertheless, there is only limited success in the exploration of nanotheranostics composing of both fluorescent dyes and PTT agents since their fluorescence would always be quenched. Herein, a facile one-pot synthesis approach of Aggregation Induced Emission (AIE) based Bi2S3 nanotheranostics is described, and their effectiveness for fluorescence imaging and simultaneous PTT has been demonstrated...
September 19, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28947889/laparoscopic-distal-resection-of-the-pancreas-can-be-all-resections-of-body-and-tail-of-the-pancreas-called-the-same
#12
Audrius Šileikis, Augustas Beiša, Virgilijus Beiša, Mindaugas Kvietkauskas, Marius Kryžauskas, Kęstutis Strupas
INTRODUCTION: Distal resection of the pancreas is a routine procedure in high-volume centres. However, the volume of this procedure can vary. This variation plays a very important role in laparoscopic approach of pancreatic surgery and can be a real challenge if the anatomical situation is underestimated. AIM OF THE STUDY: To present our experience in minimally invasive treatment of the pancreatic tumours and to discuss different approaches to different anatomical situations...
2017: Contemporary Oncology Współczesna Onkologia
https://www.readbyqxmd.com/read/28945791/use-of-real-time-cellular-analysis-and-plackett-burman-design-to-develop-the-serum-free-media-for-pc-3-prostate-cancer-cells
#13
Ai Zhao, Fahai Chen, Chunhong Ning, Haiming Wu, Huanfang Song, Yanqing Wu, Rong Chen, Kaihua Zhou, Xiaoling Xu, Yinxiang Lu, Jimin Gao
In this study, we developed a rapid strategy to screen a serum-free medium for culturing the anchorage-dependent PC-3 prostate cancer cells, which was going to be prepared in large scale to generate GM-CSF/TNFα-surface-modified whole cell prostate cancer vaccine. Automated real-time cellular analysis as a rapid and non-invasive technology was used to monitor the growth of PC-3 cells in 16-well plates. At the same time, Plackett-Burman design was employed to identify the most influential formulation by integrating relevant information statistically...
2017: PloS One
https://www.readbyqxmd.com/read/28932737/technical-intraoperative-maneuvers-for-the-management-of-inferior-vena-cava-thrombus-in-renal-cell-carcinoma
#14
Dionysios Dellaportas, Nikolaos Arkadopoulos, Ioannis Tzanoglou, Evgenios Bairamidis, George Gemenetzis, Pantelis Xanthakos, Constantinos Nastos, Georgia Kostopanagiotou, Ioannis Vassiliou, Vassilios Smyrniotis
INTRODUCTION: Renal vein or inferior vena cava (IVC) invasion by neoplastic thrombus in patients with renal cell carcinoma (RCC) is not an obstacle for radical oncological treatment. The aim of this study is to present our technical maneuvers for complete removal of the intracaval thrombus without compromising hemodymanic stability of the patient. MATERIALS AND METHODS: Between 2000 and 2014, 15 RCC patients with IVC involvement of levels I-III were treated with curative intent and were prospectively studied...
2017: Frontiers in Surgery
https://www.readbyqxmd.com/read/28904906/a-step-by-step-guide-to-office-based-sperm-retrieval-for-obstructive-azoospermia
#15
REVIEW
Robert M Coward, Jesse N Mills
A variety of surgical options exists for sperm retrieval in the setting of obstructive azoospermia (OA). With appropriate preparation, the majority of these techniques can safely be performed in the office with local anesthesia and with or without monitored anesthesia care (MAC). The available techniques include percutaneous options such as percutaneous epididymal sperm aspiration (PESA) and testicular sperm aspiration (TESA), as well as open techniques that include testicular sperm extraction (TESE) and microsurgical epididymal sperm aspiration (MESA)...
August 2017: Translational Andrology and Urology
https://www.readbyqxmd.com/read/28880433/the-effect-of-hydroxyapatite-nanoparticles-on-mechanical-behavior-and-biological-performance-of-porous-shape-memory-polyurethane-scaffolds
#16
Juhong Yu, Hong Xia, Akira Teramoto, Qing-Qing Ni
The scaffold which provides space for cell growth, proliferation and differentiation, is a key factor in bone tissue engineering. However, improvements in scaffold design are needed to precisely match the irregular boundaries of bone defects as well as facilitate clinical application. In this study, controllable three-dimensional (3D) porous shape memory polyurethane/nano-hydroxyapatite (SMPU/nHAP) composite scaffold was successfully fabricated for bone defect reparation. Detailed studies were performed to evaluate its structure, apparent density, porosity and mechanical properties, emphasizing the contribution of nHAP particles on shape recovery behaviors and biological performance in vitro...
September 7, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28876680/registrar-perceptions-on-general-surgical-training-in-south-africa-a-report-by-the-south-african-society-of-surgeons-in-training-sassit
#17
N Patel, A Leusink, N Singh, M Z Koto, T Luvhengo
BACKGROUND: Surgical training varies significantly even amongst universities within the same country. This trend is reflected in South Africa and provides an opportunity for innovation to improve the quality of general surgical training. METHOD: A prospective descriptive study was performed by means of a confidential questionnaire distributed to general surgical registrars at all 8 training centres in South Africa. Participants were asked to give comments regarding adequacy of formal academic teaching, level of supervision during surgical procedures, exposure to and training in minimally invasive surgery (MIS), and preparation for examinations...
June 2017: South African Journal of Surgery. Suid-Afrikaanse Tydskrif Vir Chirurgie
https://www.readbyqxmd.com/read/28875730/liquid-biopsy-for-brain-tumors
#18
Ganesh M Shankar, Leonora Balaj, Shannon L Stott, Brian Nahed, Bob S Carter
Minimally invasive methods will augment the clinical approach for establishing the diagnosis or monitoring treatment response of central nervous system tumors. Liquid biopsy by blood or cerebrospinal fluid sampling holds promise in this regard. Areas covered: In this literature review, the authors highlight recent studies describing the analysis of circulating tumor cells, cell free nucleic acids, and extracellular vesicles as strategies to accomplish liquid biopsy in glioblastoma and metastatic tumors. The authors then discuss the continued efforts to improve signal detection, standardize the liquid biopsy handling and preparation, develop platforms for clinical application, and establish a role for liquid biopsies in personalized medicine...
September 6, 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/28856133/pioneering-and-interprofessional-pediatric-dentistry-programs-aimed-at-reducing-oral-health-disparities
#19
Francisco Ramos-Gomez, Hamida Askaryar, Cambria Garell, Jennifer Ogren
Early Childhood Caries (ECC) is the most chronic childhood disease and more predominant in low-income and underserved children. Although easily transmitted, ECC is entirely preventable. Dr. Ramos-Gomez and his team at the University of California, Los Angeles put together an interprofessional curriculum where both medical and dental knowledge and practice is integrated to prepare dentists and primary care providers to more cost effectively address ECC and thereby reduce disparities in oral health. The curriculum, known as the Strategic Partnership for Interprofessional Collaborative Education in Pediatric Dentistry (SPICE-PD), consists of nine evidence-based training modules: applied statistics and research, community partners, interprofessional education/training, quality improvement, policy and advocacy, disease management/risk assessment, ethics/professionalism, cultural competency and children with special heath-care needs...
2017: Frontiers in Public Health
https://www.readbyqxmd.com/read/28851638/ultrasound-triggered-plga-microparticle-destruction-and-degradation-for-controlled-delivery-of-local-cytotoxicity-and-drug-release
#20
Kee W Jang, Dongrim Seol, Lei Ding, Dong Nyoung Heo, Sang Jin Lee, James A Martin, Il Keun Kwon
In this study, we investigated the low intensity ultrasound (US)-controlled delivery of local cytotoxicity and drug release via induced destruction and degradation of microparticles (MPs) made of poly(lactic-co-glycolic acid) (PLGA). This study was conducted in vitro with potential application towards tumor treatment in conjunction with direct injection. MPs, either loaded with or without doxorubicin (DOX) or blank, were prepared using a double-emulsion solvent-evaporation technique. First, the MPs were exposed to US with duty cycle (DC)-modulation...
August 26, 2017: International Journal of Biological Macromolecules
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