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https://www.readbyqxmd.com/read/28822945/development-of-an-accurate-and-sensitive-method-for-lactate-analysis-in-exhaled-breath-condensate-by-lc-ms-ms
#1
Thomas C Jackson, Yan Victoria Zhang, Patricia J Sime, Richard P Phipps, R Matthew Kottmann
Exhaled breath condensate (EBC) is easily obtained for clinical diagnosis and prognosis for pulmonary diseases and has gained much interest in biomarker discovery research and studies. Lactate, a physiological material, is found in EBC and has been demonstrated to be a potential indicator of chronic obstructive pulmonary disease and other lung diseases. Several assays are available to detect lactate in human body fluids, and yet none is suitable for detecting lactate in EBC. Due to the very low concentration of lactate in EBC and low volume of EBC, it is very important to develop an assay to measure lactate with high sensitivity, accuracy and easy sample processing...
July 24, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28822886/concurrent-spectrometry-of-annihilation-radiation-and-characteristic-gamma-rays-for-activity-assessment-of-selected-positron-emitters
#2
S G Dolley, G F Steyn, T J van Rooyen, F Szelecsényi, Z Kovács, C Vermeulen, N P van der Meulen
A method is described to determine the activity of non-pure positron emitters in a radionuclide production environment by assessing the 511keV annihilation radiation concurrently with selected γ-lines, using a single High-Purity Germanium (HPGe) detector. Liquid sources of (22)Na, (52)Fe, (52m)Mn, (61)Cu, (64)Cu, (65)Zn, (66)Ga, (68)Ga, (82)Rb, (88)Y, (89)Zr and (132)Cs were prepared specifically for this study. Acrylic absorbers surrounding the sources ensured that the emitted β(+)-particles could not escape and annihilate away from the source region...
August 1, 2017: Applied Radiation and Isotopes
https://www.readbyqxmd.com/read/28822638/modified-anterolateral-portals-in-elbow-arthroscopy-a-cadaveric-study-on-safety
#3
Stephen Thon, Peter Gold, Lane Rush, Michael J O'Brien, Felix H Savoie
PURPOSE: To evaluate the proximity to the radial nerve on cadaveric specimens of 2 modified anterolateral portals used for elbow arthroscopy. METHODS: Ten fresh cadaveric elbow specimens were prepared. Four-millimeter Steinman pins were inserted into 3 anterolateral portal sites in relation to the lateral epicondyle: (1) the standard distal anterolateral portal, (2) a modified direct anterolateral portal, and (3) a modified proximal anterolateral portal. These were defined as follows: direct portals 2 cm directly anterior to the lateral epicondyle, and proximal portals 2 cm proximal and 2 cm directly anterior to the lateral epicondyle...
August 16, 2017: Arthroscopy: the Journal of Arthroscopic & related Surgery
https://www.readbyqxmd.com/read/28822582/antibiofilm-properties-of-model-composites-containing-quaternary-ammonium-methacrylates-after-surface-texture-modification
#4
Guilherme Ferreira Rego, Marina Lermen Vidal, Gil Mendes Viana, Lucio Mendes Cabral, Luis Felipe Jochims Schneider, Maristela Barbosa Portela, Larissa Maria Cavalcante
OBJECTIVE: Investigate antimicrobial properties and surface texture of model composites with different concentration and alkyl chain length of quaternary ammonium monomers (QAS). METHODS: Monomers derived from QAS salts with alkyl chain lengths of 12 carbons ((dimethylaminododecyl methacrylate) DMADDM) and 16 carbons (dimethylaminohexadecyl methacrylate-DMAHDM) were obtained from the reactions of their respective organo-halides with the tertiary amine 2-(dimethylamino)ethyl methacrylate (DMAEMA)...
August 16, 2017: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/28822574/analysis-of-individual-and-total-microcystins-in-surface-water-by-on-line-preconcentration-and-desalting-coupled-to-liquid-chromatography-tandem-mass-spectrometry
#5
Gabriel Munoz, Sung Vo Duy, Audrey Roy-Lachapelle, Barry Husk, Sébastien Sauvé
A fast and high-throughput method is proposed for the determination of total microcystins (ΣMC) in environmental surface waters. After a 1-h Lemieux-von Rudloff oxidation step to yield the 2-methyl-3-methoxy-4-phenylbutyric acid (MMPB) moiety, samples were quenched, filtered, and directly analyzed. This was achieved via solid phase extraction (SPE) coupled on-line to ultra-high performance liquid chromatography electrospray ionization triple stage quadrupole mass spectrometry. The choice of on-line SPE settings was conducted using experimental designs...
August 5, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28822566/human-pulp-responses-to-partial-pulpotomy-treatment-with-theracal-as-compared-with-biodentine-and-proroot-mta-a-clinical-trial
#6
Hengameh Bakhtiar, Mohammad Hossein Nekoofar, Pouyan Aminishakib, Fatemeh Abedi, Fereshteh Naghi Moosavi, Ehsan Esnaashari, Arash Azizi, Samar Esmailian, Mohammad Reza Ellini, Vahid Mesgarzadeh, Mehdi Sezavar, Imad About
INTRODUCTION: Questions exist regarding the efficacy of resin-containing materials such as TheraCal directly applied on the pulp. This study sought to investigate the clinical efficacy of TheraCal as compared with Biodentine and ProRoot mineral trioxide aggregate (MTA) for partial pulpotomy. METHODS: In this clinical trial, partial pulpotomy was performed for 27 sound human maxillary and mandibular third molars scheduled for extraction. The teeth were randomly divided into 3 groups (n = 9) and underwent partial pulpotomy with TheraCal, Biodentine, and ProRoot MTA...
August 16, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28821259/the-effect-of-storage-at-ambient-temperature-on-the-feline-fecal-microbiota
#7
Moran Tal, Adronie Verbrugghe, Diego E Gomez, Charlotte Chau, J Scott Weese
BACKGROUND: Feline fecal microbiota analyses can potentially be impacted by a variety of factors such as sample preparation, sequencing method and bioinformatics analyses. Another potential influence is changes in the microbiota from storage of samples prior to processing. This study examined the effect of ambient temperature exposure on the feline fecal microbiota composition. Fecal samples were collected from 12 healthy cats, within 15 min after defecation. Samples were aliquoted and the first aliquot was frozen at -80 °C within 1 hour of defecation...
August 18, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28821222/impact-of-blood-collection-and-processing-on-peripheral-blood-gene-expression-profiling-in-type-1-diabetes
#8
Linda Yip, Rebecca Fuhlbrigge, Mark A Atkinson, C Garrison Fathman
BACKGROUND: The natural history of type 1 diabetes (T1D) is challenging to investigate, especially as pre-diabetic individuals are difficult to identify. Numerous T1D consortia have been established to collect whole blood for gene expression analysis from individuals with or at risk to develop T1D. However, with no universally accepted protocol for their collection, differences in sample processing may lead to variances in the results. Here, we examined whether the choice of blood collection tube and RNA extraction kit leads to differences in the expression of genes that are changed during the progression of T1D, and if these differences could be minimized by measuring gene expression directly from the lysate of whole blood...
August 18, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28821088/microparticles-prepared-with-50-190kda-chitosan-as-promising-non-toxic-carriers-for-pulmonary-delivery-of-isoniazid
#9
Paula M Oliveira, Breno N Matos, Priscilla A T Pereira, Taís Gratieri, Lucia H Faccioli, Marcílio S S Cunha-Filho, Guilherme M Gelfuso
Chitosan biocompatibility and mucoadhesiveness make it an ideal polymer for antituberculotic drugs microcapsulation for pulmonary delivery. Yet, previous study indicated toxicity problems to J-774.1-cells treated with some medium molecular weight (190-310kDa) chitosan microparticles. As polymer molecular weight is a crucial factor to be considered, this paper describes the preparation and characterization of chitosan (50-190kDa) microparticles containing isoniazid (INH). Cytotoxicity assays were also performed on murine peritoneal (J-774...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28821057/a-cell-penetrating-peptide-mediated-chitosan-nanocarriers-for-improving-intestinal-insulin-delivery
#10
Lei Li, Liaoqing Yang, Manman Li, Liefeng Zhang
To overcome barriers for oral delivery of insulin, the chitosan(CS)-based nanocarriers with a novel cell penetrating peptide (SAR6EW) have been prepared and evaluated in this study. Characterization measurements showed that SAR6EW/CS/insulin-NPs displayed global particles with smooth surfaces and an average diameter about 150nm. The entrapment efficiency and loading rates of insulin were 75.36% and 7.58%, respectively. Insulin could be released constantly from SAR6EW/CS/insulin-NPs in vitro. Furthermore, SAR6EW/CS/insulin-NPs could facilitate the uptake of insulin and induce a significantly higher internalization of insulin via adding clathrin and caveolae mediated endocytosis...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28821046/water-sorption-in-microfibrillated-cellulose-mfc-the-effect-of-temperature-and-pretreatment
#11
Çağlar Meriçer, Matteo Minelli, Marco Giacinti Baschetti, Tom Lindström
Water sorption behavior of two different microfibrillated cellulose (MFC) films, produced by delamination of cellulose pulp after different pretreatment methods, is examined at various temperatures (16-65°C) and up to 70% RH. The effect of drying temperature of MFC films on the water uptake is also investigated. The obtained solubility isotherms showed the typical downward curvature at moderate RH, while no upturn is observed at higher RH; the uptakes are in line with characteristic values for cellulose fibers...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28821038/the-inhibition-of-lps-induced-inflammation-in-raw264-7-macrophages-via-the-pi3k-akt-pathway-by-highly-n-acetylated-chitooligosaccharide
#12
Qingsong Xu, Meisi Liu, Qishun Liu, Wenxia Wang, Yuguang Du, Heng Yin
Chitooligosaccharide (COS) has been shown to regulate many biological functions, such as antimicrobial effect and antitumor activity. In the present study, highly N-acetylated chitooligosaccharide (NACOS) was prepared by N-acetylation of COS, and the anti-inflammatory activity of NACOS in macrophages were evaluated. The results indicated NACOS significantly suppressed the LPS-induced pro-inflammatory cytokines interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) expression. Furthermore, the increased levels of reactive oxygen species (ROS) and nitric oxide (NO) were repressed by NACOS in a dose dependent manner...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28821030/preparation-and-characterization-of-a-photocatalytic-antibacterial-material-graphene-oxide-tio2-bacterial-cellulose-nanocomposite
#13
Ling-Pu Liu, Xiao-Ning Yang, Li Ye, Dong-Dong Xue, Miao Liu, Shi-Ru Jia, Ying Hou, Li-Qiang Chu, Cheng Zhong
In this study, bacterial cellulose (BC) was used as a matrix to synthesize graphene oxide/Titanium dioxide (GOTiO2)-based hybrid materials. It was indicated by X-ray diffraction and selected area electron diffraction that the crystal structure of GOTiO2 was a mixed phase containing anatase and rutile. TiO2 nanoparticles were of 10-30nm diameters and densely anchored on graphene oxide sheets. Superior photocatalytic performance of the GOTiO2 was achieved under near UV excitation. The photocatalytic efficiency was optimized through controlling an appropriate calcined temperature...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28820982/direct-analysis-no-sample-preparation-of-bioavailable-cortisol-in-human-plasma-by-weak-affinity-chromatography-wac
#14
Sten Ohlson, Jagjit Kaur, Manfred Raida, Ulf Niss, Tim Bengala, Chester Lee Drum, Bernhard Boehm, Anthony R Torres
Pre-analytical treatment of blood plasma is a time consuming and often rate limiting step in the workflow of LC/MS analysis. We present in this pilot study a new approach for quantitative LC/MS based on weak affinity chromatography (WAC) of crude plasma. The steroid hormone cortisol was selected as a clinically relevant biomarker, as it currently requires extensive pre-analytical preparation. A WAC unit with saturating, immobilized albumin as a prototypic weak binder was used in combination with an ion-funnel MS/MS detector to perform zonal affinity chromatography of cortisol directly from a plasma sample, followed by quantitative multiple reaction monitoring (MRM)...
July 21, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28820303/rapid-ventricular-pacing-for-clip-reconstruction-of-complex-unruptured-intracranial-aneurysms-results-of-an-interdisciplinary-prospective-trial
#15
Juergen Konczalla, Johannes Platz, Stephan Fichtlscherer, Haitham Mutlak, Ulrich Strouhal, Volker Seifert
OBJECTIVE To date, treatment of complex unruptured intracranial aneurysms (UIAs) remains challenging. Therefore, advanced techniques are required to achieve an optimal result in treating these patients safely. In this study, the safety and efficacy of rapid ventricular pacing (RVP) to facilitate microsurgical clip reconstruction was investigated prospectively in a joined neurosurgery, anesthesiology, and cardiology study. METHODS Patients with complex UIAs were prospectively enrolled. Both the safety and efficacy of RVP were evaluated by recording cardiovascular events and outcomes of patients as well as the amount of aneurysm occlusion after the surgical clip reconstruction procedure...
August 18, 2017: Journal of Neurosurgery
https://www.readbyqxmd.com/read/28820049/receptor-binding-assay-for-the-detection-of-paralytic-shellfish-poisoning-toxins-comparison-to-the-mouse-bioassay-and-applicability-under-regulatory-use
#16
Shiyamalie R Ruberu, Gregg W Langlois, Melisa Masuda, Clive Kittredge, S Kusum Perera, Raphael M Kudela
The receptor-binding assay (RBA) method for the detection of paralytic shellfish poisoning (PSP) toxins was evaluated for its overall performance in comparison with the mouse bioassay (MBA). An initial study to evaluate the effects of filtering shellfish extracts prior to running the RBA indicated no significant difference between filtered and unfiltered extracts on the determined saxitoxin concentrations. Next, we tested the RBA assay on 295 naturally contaminated mussel tissue samples, ranging in concentrations from 320 µg STX equiv...
August 18, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28819975/compatibility-of-nitric-oxide-release-with-implantable-enzymatic-glucose-sensors-based-on-osmium-iii-ii-mediated-electrochemistry
#17
Kyoung Ha Cha, Mark E Meyerhoff
The compatibility of nitric oxide (NO) release coatings with implantable enzymatic glucose sensors based on osmium (III/II) mediated electrochemical detection is examined for the first time. NO-releasing osmium-mediated glucose sensors are prepared using a S-nitrosothiol impregnated outer tubing and are tested in vitro in both phosphate buffer (pH 7.4) and within whole porcine blood. It is demonstrated that after 3 days of continuous NO release at or above physiological levels, there are no negative effects on the osmium mediated elec-trochemical currents...
August 18, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28819677/direct-analysis-of-terpenes-from-biological-buffer-systems-using-sesi-and-ir-maldesi
#18
Milad Nazari, Alexandra A Malico, Måns Ekelöf, Sean Lund, Gavin J Williams, David C Muddiman
Terpenes are the largest class of natural products with a wide range of applications including use as pharmaceuticals, fragrances, flavorings, and agricultural products. Terpenes are biosynthesized by the condensation of a variable number of isoprene units resulting in linear polyisoprene diphosphate units, which can then be cyclized by terpene synthases into a range of complex structures. While these cyclic structures have immense diversity and potential in different applications, their direct analysis in biological buffer systems requires intensive sample preparation steps such as salt cleanup, extraction with organic solvents, and chromatographic separations...
August 17, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28819502/pattern-and-outcome-of-dog-bite-injuries-among-children-in-ado-ekiti-southwest-nigeria
#19
Ezra Olatunde Ogundare, Oladele Simeon Olatunya, Isaac Oluwadare Oluwayemi, Adekoya Joshua Inubile, Adekunle Bamidele Taiwo, Oyinkansola Tolulope Agaja, Alfred Airemionkhale, Adewale Fabunmi
INTRODUCTION: Dog bites in humans are a major public health problem. Globally, millions of people are bitten by dogs but most of the fatal cases occur in children. There is paucity of data on dog bite related diseases among Nigerian children. Objectives: to determine the pattern of dog bite injuries and associated health problems among children seen at Ekiti State University Teaching Hospital. METHODS: This is a retrospective study on the clinical data of patients managed for dog bite related injuries between January 2010 and June 2014...
2017: Pan African Medical Journal
https://www.readbyqxmd.com/read/28819242/the-saliva-microbiome-profiles-are-minimally-affected-by-collection-method-or-dna-extraction-protocols
#20
Yenkai Lim, Makrina Totsika, Mark Morrison, Chamindie Punyadeera
Saliva has attracted attention as a diagnostic fluid due to the association of oral microbiota with systemic diseases. However, the lack of standardised methods for saliva collection has led to the slow uptake of saliva in microbiome research. The aim of this study was to systematically evaluate the potential effects on salivary microbiome profiles using different methods of saliva collection, storage and gDNA extraction. Three types of saliva fractions were collected from healthy individuals with or without the gDNA stabilising buffer...
August 17, 2017: Scientific Reports
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