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https://www.readbyqxmd.com/read/28527844/evaluation-of-various-pretreatments-to-fiber-post-on-the-push-out-bond-strength-of-root-canal-dentin
#1
Ömer Kırmalı, Özlem Üstün, Alper Kapdan, Alper Kuştarcı
INTRODUCTION: The aim of this in vitro study was to compare the effect of different pretreatments (fiber post) with the laser-activated irrigation (LAI) technique (for removal of the smear layer) on root canal dentin in terms of push-out bond strength (PBS) in a fiber post. METHODS: Fifty freshly extracted mandibular single-rooted premolars were prepared, and LAI was applied to all roots (17% EDTA was 5 mL for 120 seconds with an erbium, chromium:yttrium-scandium-gallium-garnet laser [0...
May 17, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28527780/-youtube-as-a-source-of-spinal-anesthesia-epidural-anesthesia-and-combined-spinal-and-epidural-anesthesia
#2
Serkan Tulgar, Onur Selvi, Talat Ercan Serifsoy, Ozgur Senturk, Zeliha Ozer
INTRODUCTION: Social media as YouTube have become a part of daily life and many studies evaluated health-related YouTube videos. Our aim was to evaluate videos available on YouTube for the conformity to textbook information and their sufficiency as a source for patient information. MATERIAL AND METHOD: A search of the YouTube website was performed using the keywords "spinal anesthesia, epidural anesthesia, combined spinal epidural anesthesia". Firstly, 180 videos were evaluated and the characteristics of the video were noted, and the features of the video too were noted if the video was regarding neuraxial anesthesia...
May 17, 2017: Revista Brasileira de Anestesiologia
https://www.readbyqxmd.com/read/28527631/the-thiol-switch-c684-in-mitofusin-2-mediates-redox-induced-alterations-of-mitochondrial-shape-and-respiration
#3
Osamah Thaher, Christina Wolf, Partha Narayan Dey, Alireza Pouya, Verena Wüllner, Stefan Tenzer, Axel Methner
Mitofusin-2 (MFN2) is a GTPase in the outer mitochondrial membrane involved in the regulation of mitochondrial fusion and bioenergetics. MFN2 also plays a role in mitochondrial fusion induced by changes in the intracellular redox state. Adding oxidized glutathione (GSSG), the core cellular stress indicator, to mitochondrial preparations stimulates mitochondrial fusion by inducing disulphide bond-mediated oligomer formation of MFN2 and its homolog MFN1 which involve cysteine 684 (C684) of MFN2. Mitochondrial hyperfusion represents an adaptive stress response that confers transient protection by increasing mitochondrial ATP production but how this depends on the thiol switch C684 in MFN2 has not been investigated...
May 17, 2017: Neurochemistry International
https://www.readbyqxmd.com/read/28527527/small-scale-separation-of-isoxazole-structurally-related-analogues-by-chiral-supercritical-fluid-chromatography
#4
Yasmine Zehani, Lucas Lemaire, Regis Millet, Emmanuelle Lipka
Chromatographic preparative enantioseparation is now the preferred method to obtain milligram amounts of pure enantiomers in the first step of the development of a therapeutic molecule. Supercritical fluid chromatography has many advantages over liquid chromatography and was therefore chosen for the small scale enantioseparation of four original 3-carboxamido-5-aryl isoxazole molecules, ligands of the CB2 cannabinoid receptors. The preparation of about 10mg of each of the eight enantiomers was achieved successfully on a Chiralpak(®) AD-H (tris-3,5-dimethylphenylcarbamate of amylose) polysaccharide based stationary phase with various percentages of ethanol as a co-solvent, through mixed-stream injections and touching-band approach...
May 12, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28527427/photothermal-therapy-using-folate-conjugated-gold-nanoparticles-enhances-the-effects-of-6mv-x-ray-on-mouth-epidermal-carcinoma-cells
#5
Ali Neshastehriz, Mousa Tabei, Shayan Maleki, Samira Eynali, Ali Shakeri-Zadeh
The aim of this study was to develop an optimized method for preparation of folate conjugated gold nanoparticles (F-AuNPs) and to investigate its cytotoxic effects and cell apoptosis in combination with photothermal therapy (PTT) and radiotherapy (RT) for the treatment of mouth epidermal carcinoma cells KB. For this purpose, cells were treated with synthesized F-AuNPs at different concentrations for 6h and then irradiated them with laser beam (532nm, 0.5W/cm(2), 15min). After photothermal therapy, the cells were exposed to 6MV X-ray with a single dose of 2Gy...
May 12, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28527354/incorporation-of-silver-nanoparticles-into-magnetron-sputtered-calcium-phosphate-layers-on-titanium-as-an-antibacterial-coating
#6
Maria A Surmeneva, Anna A Sharonova, Svitlana Chernousova, Oleg Prymak, Kateryna Loza, Mikhail S Tkachev, Ivan A Shulepov, Matthias Epple, Roman A Surmenev
A three-layer system of nanocrystalline hydroxyapatite (first layer; 1000nm thick), silver nanoparticles (second layer; 1.5μg Ag cm(-2)) and calcium phosphate (third layer, either 150 or 1000nm thick) on titanium was prepared by a combination of electrophoretic deposition of silver nanoparticles and the deposition of calcium phosphate by radio frequency magnetron sputtering. Scanning electron microscopy showed that the silver nanoparticles were evenly distributed over the surface. The adhesion of multilayered coating on the substrate was evaluated using the scratch test method...
May 9, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28526348/chitosan-co-hyaluronic-acid-porous-cryogels-and-their-application-in-tissue-engineering
#7
Tuğçe Kutlusoy, Burcu Oktay, Nilhan Kayaman Apohan, Mediha Süleymanoğlu, Serap Erdem Kuruca
In this work, the usability of chitosan-co-hyaluronic acid cryogels as a tissue-engineering scaffold was investigated. Chitosan-co-hyaluronic acid cryogels were synthesized at subzero temperature. Cryogels which were composed of various compositions of chitosan and hyaluronic acid (0, 10, 20, 30 and 50wt % hyaluronic acid) was prepared. Morphological studies showed that the macroporous cryogels have have been developed with 90-95% porosity. Particularly, the mechanical and biomaterial property of pure chitosan was improved by making copolymer with hyaluronic acid in different concentration...
May 16, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28526346/release-study-and-inhibitory-activity-of-thyme-essential-oil-loaded-chitosan-nanoparticles-and-nanocapsules-against-foodborne-bacteria
#8
M Sotelo-Boyás, Z Correa-Pacheco, S Bautista-Baños, Y Gómez Y Gómez
The antibacterial property of thyme essential oil due to different volatile compounds, has been well documented in the literature. To overcome the high volatility of essential oil components, encapsulation has emerged as a new alternative. In this work, chitosan and thyme essential oil-loaded chitosan nanoparticles (TEO-CSNPs) and nanocapsules (TEO-CSNCs) were prepared by nanoprecipitation and nanoencapsulation, respectively. The morphology, encapsulation efficiency, release kinetics, and inhibitory activity were evaluated...
May 16, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28526278/biotherapeutics-in-chronic-rhinosinusitis-with-and-without-nasal-polyps
#9
Claus Bachert, Philippe Gevaert, Peter Hellings
In western countries, 85% of chronic rhinosinusitis with nasal polyp disease reveals a type 2 inflammatory pattern with expression of IL-4, -5, and -13 as well as increased concentrations of IgE, whereas chronic rhinosinusitis without nasal polyps merely expresses these biomarkers. The degree of type 2 inflammation furthermore is associated with disease severity, asthma comorbidity, and recurrence of disease after surgery. Therefore, these biomarkers also form the targets for innovative therapeutic approaches such as monoclonal antibodies directed against IgE, IL-5, and IL-4 receptor alpha, blocking the activity of IL-4 and -13...
May 16, 2017: Journal of Allergy and Clinical Immunology in Practice
https://www.readbyqxmd.com/read/28525862/application-of-nirs-coupled-with-pls-regression-as-a-rapid-non-destructive-alternative-method-for-quantification-of-kba-in-boswellia-sacra
#10
Ahmed Al-Harrasi, Najeeb Ur Rehman, Fazal Mabood, Muhammaed Albroumi, Liaqat Ali, Javid Hussain, Hidayat Hussain, René Csuk, Abdul Latif Khan, Tanveer Alam, Saif Alameri
In the present study, for the first time, NIR spectroscopy coupled with PLS regression as a rapid and alternative method was developed to quantify the amount of Keto-β-Boswellic Acid (KBA) in different plant parts of Boswellia sacra and the resin exudates of the trunk. NIR spectroscopy was used for the measurement of KBA standards and B. sacra samples in absorption mode in the wavelength range from 700-2500nm. PLS regression model was built from the obtained spectral data using 70% of KBA standards (training set) in the range from 0...
May 10, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28525809/one-step-preparation-of-nanostructured-martite-catalyst-and-graphite-electrode-by-glow-discharge-plasma-for-heterogeneous-electro-fenton-like-process
#11
Alireza Khataee, Saeed Sajjadi, Aliyeh Hasanzadeh, Behrouz Vahid, Sang Woo Joo
Natural Martite ore particles and graphite were modified by alternating current (AC) glow discharge plasma to form nanostructured catalyst and cathode electrode for using in the heterogeneous-electro Fenton-like (Het-EF-like) process. The performance of the plasma-treated martite (PTM) and graphite electrode (PTGE) was studied for the treatment of paraquat herbicide in a batch system. 85.78% degradation efficiency for 20 mg L(-1) paraquat was achieved in the modified process under desired operational conditions (i...
May 16, 2017: Journal of Environmental Management
https://www.readbyqxmd.com/read/28525340/detection-of-bordetella-avium-by-taqman-real-time-pcr-in-tracheal-swabs-from-wildlife-birds
#12
T Stenzel, D Pestka, B Tykałowski, M Śmiałek, A Koncicki, A Bancerz-Kisiel
Bordetella avium, the causing agent of bordetellosis, a highly contagious infection of the respiratory tract in young poultry, causes significant losses in poultry farming throughout the world. Wildlife birds can be a reservoir of various pathogens that infect farm animals. For this reason the studies were conducted to estimate the prevalence of Bordetella avium in wildlife birds in Poland. Tracheal swab samples were collected from 650 birds representing 27 species. The bacterial DNA was isolated directly from the swabs and screened for Bordetella avium by TaqMan real-time PCR...
March 28, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28525319/preparation-for-sexual-and-reproductive-self-care-in-iranian-adolescent-girls-a-qualitative-study
#13
Zainab Alimoradi, Nourossadat Kariman, Fazlollah Ahmadi, Masoumeh Simbar
Background Adolescence is one of the most important stages in every individual's life. Pubertal changes and acquiring reproduction capability require adolescents to perform special health care processes. Also the possibility of involvement in high-risk sexual behaviors endangers adolescent girls' sexual and reproductive health. Objective Increase and deepen the understanding and knowledge of the factors affecting Iranian adolescent girls' readiness to take care of their sexual and reproductive health. Materials and methods The present qualitative study was conducted using conventional content analysis method...
May 17, 2017: International Journal of Adolescent Medicine and Health
https://www.readbyqxmd.com/read/28524783/quantitative-analysis-of-veterinary-drugs-in-bovine-muscle-and-milk-by-liquid-chromatography-quadrupole-time-of-flight-mass-spectrometry
#14
Shizuka Saito-Shida, Takatoshi Sakai, Satoru Nemoto, Hiroshi Akiyama
A simple and reliable multiresidue method for quantitative determination of veterinary drugs in bovine muscle and milk using liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QTOF-MS) was developed. Critical MS parameters such as capillary voltage, cone voltage, collision energy, desolvation gas temperature, and extraction mass window were carefully optimised to obtain the best possible sensitivity. Analytical samples were prepared using extraction with acetonitrile and hexane in the presence of anhydrous sodium sulphate and acetic acid, followed by ODS cartridge clean-up...
May 19, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28524415/ndrg4-an-early-detection-marker-for-colorectal-cancer-is-specifically-expressed-in-enteric-neurons
#15
N Vaes, M H F M Lentjes, M J Gijbels, G Rademakers, K L Daenen, W Boesmans, K A D Wouters, A Geuzens, X Qu, H P J Steinbusch, B P F Rutten, S H Baldwin, K A Sharkey, R M W Hofstra, M van Engeland, P Vanden Berghe, V Melotte
BACKGROUND: Promoter methylation of N-myc Downstream-Regulated Gene 4 (NDRG4) in fecal DNA is an established early detection marker for colorectal cancer (CRC). Despite its connection to CRC, NDRG4 is predominantly studied in brain and heart, with little to no knowledge about its expression or role in other organs. In this study, we aimed to determine the whole-body expression of NDRG4, with a focus on the intestinal tract. METHODS: We investigated NDRG4 expression throughout the body by immunohistochemistry, Western Blotting and in situ mRNA hybridization using tissues from NDRG4 wild-type, heterozygous and knockout mice and humans...
May 19, 2017: Neurogastroenterology and Motility: the Official Journal of the European Gastrointestinal Motility Society
https://www.readbyqxmd.com/read/28524265/a-highly-sensitive-resonance-rayleigh-scattering-and-colorimetric-assay-for-the-recognition-of-propranolol-in-%C3%AE-adrenergic-blocker
#16
Xuanping Tan, Jidong Yang, Qiong Yang, Qin Li
In this work, a highly sensitive, citrate anion-capped gold nanoparticles (AuNPs)-based assay for the determination of propranolol in real samples with resonance Rayleigh scattering (RRS) and colorimetry was developed. When AuNPs were prepared by the sodium citrate reduction method, citrate anions self-assembled on the surface of AuNPs to form supramolecular complex anions. In BR 4.6 buffer solution, propranolol was positively charged and could bind with AuNPs to form larger aggregates through electrostatic force and hydrophobic effects...
May 19, 2017: Luminescence: the Journal of Biological and Chemical Luminescence
https://www.readbyqxmd.com/read/28524193/high-ph-instability-of-quaternary-ammonium-surfactant-coatings-in-capillary-electrophoresis
#17
Lisa Shulman, Lei Pei, Mahmoud F Bahnasy, Charles A Lucy
The two-tailed cationic surfactant dioctadecyldimethyl ammonium bromide (DODAB) produces semi-permanent coatings that yield strongly reversed electroosmotic flow (EOF), for example -0.31 ± 0.01 cm(2) kV(-1) s(-1) at pH 3.5. Moreover, these coatings are easy to prepare, regenerable, cost effective, and yield high efficiency (520 000-900 000 plates per m) separations of cationic proteins over many runs under acidic (pH 3.5) conditions. Given the quaternary amine functionality of DODAB, we were surprised to observe that DODAB coatings become unstable at pH > 7...
May 19, 2017: Analyst
https://www.readbyqxmd.com/read/28523910/dense-layer-of-bacteriophages-ordered-in-alternating-electric-field-and-immobilized-by-surface-chemical-modification-as-sensing-element-for-bacteria-detection
#18
Łukasz Richter, Krzysztof Bielec, Adam Lesniewski, Marcin Łoś, Jan Paczesny, Robert Holyst
Faster and more sensitive environmental monitoring should be developed to face the worldwide problem of bacterial infections. To remedy this issue we demonstrate bacteria sensing element, which utilizes dense and ordered layers of bacteriophages specific for given bacteria strain. We combine 1) chemical modification of the surface to increase the surface coverage of bacteriophages 2) with alternating electric field to greatly increase the number of properly oriented bacteriophages at the surface. Usually in sensing elements a random orientation of bacteriophages results in steric hindrances, which cause that no more than few percent of all receptors is available...
May 19, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28523846/a-novel-rapid-analysis-using-mass-spectrometry-to-evaluate-downstream-refolding-of-recombinant-human-insulin-like-growth-factor-1-mecasermin
#19
Kenichiro Furuki, Toshimasa Toyo'oka, Hideto Yamaguchi
RATIONALE: Mecasermin is used to treat elevated blood sugar as well as growth hormone-resistant Laron-type dwarfism. Mecasermin isolated from inclusion bodies in extracts of E.coli must be refolded to acquire sufficient activity. However, there is no rapid analytical method for monitoring refolding during the purification process. METHODS: We prepared mecasermin drug product, in-process samples during the oxidation of mecasermin, forced-reduced mecasermin, and aerially oxidized mecasermin after forced reduction...
May 19, 2017: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/28523615/a-facile-route-for-preparation-of-magnetic-biomass-activated-carbon-with-high-performance-for-removal-of-dye-pollutants
#20
Hao Luo, Shengxiao Zhang, Xiaoyan Li, Qiang Xu, Junshen Liu, Zhenhua Wang
A novel and simple method for preparing magnetic biomass activated carbon (BAC) was developed. The BAC was prepared by decomposing fallen leaves, and magnetic nanoparticles were grown in situ on BAC using solvothermal method. The prepared magnetic BAC was characterized with FT-IR, XRD, vibrating sample magnetometer, thermo-gravimetric apparatus, SEM, and high-resolution transmission electron microscope, and results indicate that BAC and magnetic nanoparticles were combined together successfully. To investigate the adsorption ability of the composites, several dyes were selected as sample pollutants, and the sorbent showed high adsorption capacity for the dyes...
May 18, 2017: Environmental Science and Pollution Research International
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