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https://www.readbyqxmd.com/read/29330789/fluorescence-lifetime-imaging-microscopy-flim-as-a-tool-to-investigate-hypoxia-induced-protein-protein-interaction-in-living-cells
#1
Vera Schützhold, Joachim Fandrey, Katrin Prost-Fingerle
Fluorescence resonance energy transfer (FRET) is widely used as a method to investigate protein-protein interactions in living cells. A FRET pair donor fluorophore in close proximity to an appropriate acceptor fluorophore transfers emission energy to the acceptor, resulting in a shorter lifetime of the donor fluorescence. When the respective FRET donor and acceptor are fused with two proteins of interest, a reduction in donor lifetime, as detected by fluorescence lifetime imaging microscopy (FLIM), can be taken as proof of close proximity between the fluorophores and therefore interaction between the proteins of interest...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29328372/differential-microrna-expression-profiles-and-bioinformatics-analysis-between-young-and-aging-spontaneously-hypertensive-rats
#2
Jingfeng Wang, Jingjing Zhang, Xuefeng Ding, Yanyan Wang, Zhiming Li, Weipeng Zhao, Jianguo Jia, Jingmin Zhou, Junbo Ge
MicroRNAs (miRNAs/miRs) serve a role as important regulators in cardiac hypertrophy. The present study aimed to reveal the differential expression profile of miRNAs between young and aging spontaneously hypertensive rats (SHRs) and studied the functional annotation of predicted targets. Briefly, 3‑month‑old and 12‑month‑old SHRs (n=3/group) were subjected to echocardiography, histopathological analysis and dihydroethidium staining. Subsequently, small RNA sequencing and data processing was conducted to identify the differentially expressed miRNAs between these two groups...
January 9, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29318974/-understanding-the-role-of-hypoxia-inducible-factor-during-neurodegeneration-for-new-therapeutics-opportunities
#3
Amalia Merelli, Julio Cesar Garcia Rodriguez, Jaume Folch, Marcelo R Regueiro, Antoni Camins, Lazarowski Alberto
Neurodegeneration (NDG) is linked with the progressive loss of neural function with intellectual and/or motor impairment. Several diseases affecting older individuals, including Alzheimer's disease, Amyotrophic Lateral Sclerosis, Huntington's disease, Parkinson's disease, stroke, Multiple Sclerosis and many others, are the most relevant disorders associated with NDG. Since other pathologies such as refractory epilepsy, brain infections, or hereditary diseases such as "neurodegeneration with brain iron accumulation", also lead to chronic brain inflammation with loss of neural cells, NDG can be said to affect all ages...
January 10, 2018: Current Neuropharmacology
https://www.readbyqxmd.com/read/29304733/cobalt-chloride-compromises-transepithelial-barrier-properties-of-caco-2-bbe-human-gastrointestinal-epithelial-cell-layers
#4
K M DiGuilio, M C Valenzano, E Rybakovsky, J M Mullin
BACKGROUND: Elevation of the transcription factor HIF-1 is a prominent mediator of not only processes that accompany hypoxia, but also the tumor microenvironment and tissue regeneration. This study uses mediators of "chemical hypoxia" to ask the question whether HIF-1α elevation in a healthy epithelial cell layer leads to leakiness in its tight junctional seals. METHODS: Transepithelial electrical resistance and transepithelial diffusion of 14C-D-mannitol and other radiolabeled probes are used as indicators of transepithelial barrier function of CaCo-2 BBe human gastrointestinal epithelial cell layers cultured on permeable supports...
January 5, 2018: BMC Gastroenterology
https://www.readbyqxmd.com/read/29300322/mutations-in-emt-related-genes-in-alk-positive-crizotinib-resistant-non-small-cell-lung-cancers
#5
Jiacong Wei, Anthonie J van der Wekken, Ali Saber, Miente M Terpstra, Ed Schuuring, Wim Timens, T Jeroen N Hiltermann, Harry J M Groen, Anke van den Berg, Klaas Kok
Crizotinib is an effective drug for patients with anaplastic lymphoma kinase (ALK)-positive non-small-cell lung cancer (NSCLC), but upon treatment, the tumors inevitably become crizotinib resistant in time. The resistance mechanisms are only partly understood. In this study, we aim to identify gene mutations associated with resistance in ALKpositive advanced non-squamous NSCLC treated with crizotinib. Four ALK positive patients with progressive disease following crizotinib treatment were identified with paired pre- and post-crizotinib tumor tissue from our previously published cohort...
January 4, 2018: Cancers
https://www.readbyqxmd.com/read/29298510/structural-activity-relationship-of-ginsenosides-from-steamed-ginseng-in-the-treatment-of-erectile-dysfunction
#6
Ang Ying, Qing-Tao Yu, Li Guo, Wen-Song Zhang, Jin-Feng Liu, Yun Li, Hong Song, Ping Li, Lian-Wen Qi, Ya-Zhong Ge, E-Hu Liu, Qun Liu
Ginseng has been reported to have diverse pharmacological effects. One of the therapeutic claims for ginseng is to enhance sexual function. Ginsenosides are considered as the major active constituents. A steaming process can alter the ginsenoside profile of ginseng products. The structure-function relationship of ginsenosides in the treatment of erectile dysfunction (ED) has not been investigated yet. In this work, 15 different processed ginsengs are produced by steaming, and 13 major ginsensosides are quantified by liquid chromatography with UV detection, including Rg1, Re, Rf, Rb1, Rc, Rb2, Rf, Rk3, Rh4, 20S-Rg3, 20R-Rg3, Rk1, and Rg5...
January 3, 2018: American Journal of Chinese Medicine
https://www.readbyqxmd.com/read/29295567/minoxidil-induction-of-vegf-is-mediated-by-inhibition-of-hif-prolyl-hydroxylase
#7
Soohwan Yum, Seongkeun Jeong, Dohoon Kim, Sunyoung Lee, Wooseong Kim, Jin-Wook Yoo, Jung-Ae Kim, Oh Sang Kwon, Dae-Duk Kim, Do Sik Min, Yunjin Jung
The topical application of minoxidil may achieve millimolar concentrations in the skin. We investigated whether millimolar minoxidil could induce vascular endothelial growth factor (VEGF), a possible effector for minoxidil-mediated hair growth, and how it occurred at the molecular level. Cell-based experiments were performed to investigate a molecular mechanism underlying the millimolar minoxidil induction of VEGF. The inhibitory effect of minoxidil on hypoxia-inducible factor (HIF) prolyl hydroxylase-2 (PHD-2) was tested by an in vitro von Hippel-Lindau protein (VHL) binding assay...
December 25, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29289482/the-correlation-between-hif-1-alpha-and-vegf-in-oral-squamous-cell-carcinomas-expression-patterns-and-quantitative-immunohistochemical-analysis
#8
Li-Tzu Lee, Yong-Kie Wong, Chan Man-Yee, Kuo-Wei Chang, Shyh-Chang Chen, Chiou-Tuz Chang, John Wang
BACKGROUND: The prognostic predict biomarkers are important in oral squamous cell carcinoma (OSCC). We investigated the expression patterns and quantitation of hypoxia-inducible factor-1 alpha (HIF-1α) and vascular endothelial growth factor (VEGF) in OSCC patients. Among OSCC patients with recurrence and metastasis, the expression percentages of HIF-1α and VEGF also were analyzed. METHODS: Thirty-eight patients (8 hyperkeratosis, 30 oral squamous cell carcinoma) were included in this study...
December 27, 2017: Journal of the Chinese Medical Association: JCMA
https://www.readbyqxmd.com/read/29285833/regulatory-mechanisms-of-hif-1-activity-two-decades-of-knowledge
#9
REVIEW
Sho Koyasu, Minoru Kobayashi, Yoko Goto, Masahiro Hiraoka, Hiroshi Harada
Hypoxia-inducible factor 1 (HIF-1) is a transcriptional activator of various genes related to cellular adaptive responses to hypoxia. Dysfunctions in the regulatory systems of HIF-1 activity have been implicated in the pathogenesis of various diseases including malignant tumors, and, thus, elucidating the molecular mechanisms underlying the activation of HIF-1 is eagerly desired for the development of novel anti-cancer strategies. The importance of the oxygen-dependent and ubiquitin-mediated proteolysis of the regulatory subunit of HIF-1 (HIF-1α) was first reported in 1997...
December 29, 2017: Cancer Science
https://www.readbyqxmd.com/read/29284117/knockdown-of-ampk%C3%AE-decreases-atm-expression-and-increases-radiosensitivity-under-hypoxia-and-nutrient-starvation-in-an-sv40-transformed-human-fibroblast-cell-line-lm217
#10
Yasuhiko Murata, Takuma Hashimoto, Yusuke Urushihara, Soichiro Shiga, Kazuya Takeda, Keiichi Jingu, Yoshio Hosoi
BACKGROUND: Presence of unperfused regions containing cells under hypoxia and nutrient starvation contributes to radioresistance in solid human tumors. It is well known that hypoxia causes cellular radioresistance, but little is known about the effects of nutrient starvation on radiosensitivity. We have reported that nutrient starvation induced decrease of mTORC1 activity and decrease of radiosensitivity in an SV40-transformed human fibroblast cell line, LM217, and that nutrient starvation induced increase of mTORC1 activity and increase of radiosensitivity in human liver cancer cell lines, HepG2 and HuH6 (Murata et al...
December 25, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29278273/hypoxia-inducible-factor-1-hif-1-inhibitors-from-the-last-decade-2007-to-2016-a-structure-activity-relationship-perspective
#11
REVIEW
Deepak Bhattarai, Xuezhen Xu, Kyeong Lee
Tumor hypoxia is a common feature in most solid tumors and is associated with overexpression of the hypoxia response pathway. Overexpression of the hypoxia-inducible factor (HIF-1) protein leads to angiogenesis, metastasis, apoptosis resistance, and many other pro-tumorigenic responses in cancer development. HIF-1 is a promising target in cancer drug development to increase the patient's response to chemotherapy and radiotherapy as well as the survival rate of cancer patients. Since up to 1% of genes are hypoxia-sensitive, a target-specific HIF-1 inhibitor may be a better clinical candidate in cancer drug discovery...
December 26, 2017: Medicinal Research Reviews
https://www.readbyqxmd.com/read/29260521/-research-on-relationship-of-hif-1-signaling-pathway-and-postmenstrual-osteoporosis
#12
Hang Zhong, Can Cao, Jing Yang, Qiang Huang
OBJECTIVE: Exploring the role of hypoxia-induceibal factor-1 (HIF-1) signaling pathway in postmenstrual osteoporosis (PMOP) and how to play a role in PMOP. METHODS: Sixty C57BL/6J female mouse were randomly divided into 4 groups: sham-operation group (A group),ovariectomized PMOP group (B group),HIF-1 alpha inhibitor 2-methoxy estradiol (2ME2) treatment group(D group) and solvent control group(C group),15 mice in each group. There months after modeling,the metabolism product of mouse bone tissue including serum propeptide of typeⅠ procollagen (PINP),C-terminal telopeptide-Ⅰ (CTX-1) and serum estrogen were quantified by enzyme-linked immunosorbent assay (ELISA)...
November 2017: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/29247718/important-signals-regulating-coronary-artery-angiogenesis
#13
REVIEW
Sara Shoeibi, Paul Mozdziak, Shabnam Mohammadi
Angiogenesis is a complex process of budding, the formation of new blood vessels from pre-existing microvessels, via migration, proliferation and survival. Vascular angiogenesis factors include different classes of molecules that have a fundamental role in blood vessel formation. Numerous inducers of angiogenesis, such as the members of the vascular endothelial growth factor (VEGF) family, basic fibroblast growth factor (bFGF), angiopoietin (Ang), hepatocyte growth factor (HGF), and hypoxia inducible factor-1 (HIF-1), have an important role in angiogenesis...
December 13, 2017: Microvascular Research
https://www.readbyqxmd.com/read/29245898/a-novel-small-molecule-arylsulfonamide-causes-energetic-stress-and-suppresses-breast-and-lung-tumor-growth-and-metastasis
#14
Xin Dai, Stefan Kaluz, Ying Jiang, Lei Shi, DeAngelo Mckinley, Yingzhe Wang, Binghe Wang, Erwin G Van Meir, Chalet Tan
Neoplastic cells display reprogrammed metabolism due to the heightened energetic demands and the need for biomass synthesis of a growing tumor. Targeting metabolic vulnerabilities is thus an important goal for cancer therapy. Here, we describe a novel small-molecule arylsulfonamide (N-cyclobutyl-N-((2,2-dimethyl-2H-pyrano[3,2-b]pyridin-6-yl)methyl)-3,4-dimethoxybenzenesulfonamide) that exerts potent cytotoxicity and energetic stress on tumor cells while largely sparing non-cancerous human cells. In tumor cells, it stimulates glycolysis and accelerates glucose consumption...
November 21, 2017: Oncotarget
https://www.readbyqxmd.com/read/29242064/hypoxia-inducible-factor-1-in-clinical-and-experimental-aortic-aneurysm-disease
#15
Wei Wang, Baohui Xu, Haojun Xuan, Yingbin Ge, Yan Wang, Lixin Wang, Jianhua Huang, Weiguo Fu, Sara A Michie, Ronald L Dalman
OBJECTIVE: Mural angiogenesis and macrophage accumulation are two pathologic hallmarks of abdominal aortic aneurysm (AAA) disease. The heterodimeric transcription factor hypoxia-inducible factor 1 (HIF-1) is an essential regulator of angiogenesis and macrophage function. In this study, we investigated HIF-1 expression and activity in clinical and experimental AAA disease. METHODS: Human aortic samples were obtained from 24 AAA patients and six organ donors during open abdominal surgery...
December 11, 2017: Journal of Vascular Surgery
https://www.readbyqxmd.com/read/29233858/enhancement-of-glycolysis-by-inhibition-of-oxygen-sensing-prolyl-hydroxylases-protects-alveolar-epithelial-cells-from-acute-lung-injury
#16
Kentaro Tojo, Nao Tamada, Yusuke Nagamine, Takuya Yazawa, Shuhei Ota, Takahisa Goto
Cellular bioenergetic failure caused by mitochondrial dysfunction is a key process of alveolar epithelial injury during acute respiratory distress syndrome (ARDS). Prolyl hydroxylases (PHDs) act as cellular oxygen sensors, and their inhibition activates hypoxia-inducible factor (HIF), resulting in enhanced cellular glycolytic activity, which could compensate for impaired mitochondrial function and protect alveolar epithelial cells from ARDS. Here, we evaluated the effects of pharmacological PHD inhibition with dimethyloxalylglycine (DMOG) on alveolar epithelial cell injury using in vitro and in vivo ARDS models...
December 12, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29230103/oxygen-supplementation-ameliorates-tibial-development-via-stimulating-vascularization-in-tibetan-chickens-at-high-altitudes
#17
Shucheng Huang, Xiaole Tong, Mujeeb Ur Rehman, Meng Wang, Lihong Zhang, Lei Wang, Jiakui Li, Shijin Yang
Tibetan chickens (TBCs) living in high-altitude hypoxic environment, are characterized by delayed growth and small size as compared to low-altitude broiler chickens. Increasing evidences signify the beneficial effect of oxygen (O2) supplementation in animal's body for regulating their body growth and organ development. However, it is still unclear that whether O2 supplementation has an ameliorative and protective role in TBCs living at high altitude. In this study, we first found that O2 supplementation not only increased the survival rate but also promoted the growth of TBCs associated with bone development...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/29225432/molecular-docking-based-screening-of-a-simulated-hif-1-protein-model-for-potential-inhibitors
#18
Mundla Sri Latha, Madhu Sudhana Saddala
Hypoxia inducible factor-1(HIF-1) is a bHLH-family transcription factor that control genes involved in glucolysis, angiogenesis, migration, as well as invasion factors that are important for tumor progression and metastasis. HIF-1, a hetero dimer of HIF-1α and HIF-1β, binds to the hypoxia responsive genes, such as vascular endothelial growth factor (VEGF). It is one the molecular target for angiogenesis. A series of Chalcone - like compounds described that preferentially inhibit HIF-1 dimer, which can interact with amino acids within the active site of the protein...
2017: Bioinformation
https://www.readbyqxmd.com/read/29209984/intravesical-thalidomide-boosts-bacillus-calmette-gu%C3%A3-rin-bcg-in-non-muscle-invasive-bladder-cancer-treatment
#19
Gabriela R Passos, Juliana A Camargo, Karen L Ferrari, Mário J A Saad, Amilcar C de Mattos, Leonardo O Reis
The aim of this study was to explore the efficacy of intravesical Thalidomide (immunomodulatory, anti-inflammatory and anti-angiogenic) added to BCG using an immune competent autochthonous orthotopic NMIBC animal model. Female Fischer 344 rats, 7 weeks of age, received every 2 weeks for four times, a dose of 1.5 mg/kg of N-methyl-N-nitrosourea (MNU) intravesically. The rats were randomized into four groups (n = 10 per group) to receive intravesical treatment once a week for 6 weeks as follows: control (0...
December 4, 2017: Medical Oncology
https://www.readbyqxmd.com/read/29189156/post-stroke-constraint-induced-movement-therapy-increases-functional-recovery-angiogenesis-and-neurogenesis-with-enhanced-expression-of-hif-1%C3%AE-and-vegf
#20
Ce Li, Bei Zhang, Yulian Zhu, Yingying Li, Peile Liu, Beiyao Gao, Shan Tian, Liang Du, Yu-Long Bai
BACKGROUND: Constraint-Induced Movement Therapy (CIMT) is one efficient approach to improve functional recovery after ischemic stroke. The underlying molecular mechanism remains unclear. In the current study, we investigated the effects of CIMT on angiogenesis and neurogenesis. To start linking our findings to molecular mediators, we further examined the expression of hypoxia-inducible factor-1α (HIF-1α), factor inhibiting HIF-1 (FIH-1) and vascular endothelial growth factor (VEGF)...
November 28, 2017: Current Neurovascular Research
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