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flow cytometry immunophenotyping

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https://www.readbyqxmd.com/read/29762555/global-transcriptome-analysis-identifies-weight-regain-induced-activation-of-adaptive-immune-responses-in-white-adipose-tissue-of-mice
#1
D S Kyung, H R Sung, Y J Kim, K D Kim, S Y Cho, J H Choi, Y-H Lee, I Y Kim, J K Seong
OBJECTIVE: Studies have indicated that weight regain following weight loss predisposes obese individuals to metabolic disorders; however, the molecular mechanism of this potential adverse effect of weight regain is not fully understood. Here we investigated global transcriptome changes and the immune response in mouse white adipose tissue caused by weight regain. DESIGN: We established a diet switch protocol to compare the effects of weight regain with those of weight gain without precedent weight loss, weight loss maintenance and chow diet...
December 7, 2017: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/29754509/-new-and-traditional-directions-in-the-biology-and-management-of-childhood-acute-lymphoblastic-leukemia
#2
Bálint Egyed, Gábor Kovács, Nóra Kutszegi, Andrea Rzepiel, Judit Csányiné Sági, Dániel János Erdélyi, Judit Müller, Ágnes Félné Semsei
Owing to clinical trials and improvement over the past few decades, the majority of children with acute lymphoblastic leukemia (ALL) survive by first-line chemotherapy and combat with the problems of returning to community. However, many patients may have severe acute or late therapeutic side effects, and the survival rate in some groups (e.g., patients with MLL rearrangements, hypodiploidy, IKZF1 mutation or early precursor T cell phenotype) is far behind the average. Innovative strategies in medical attendance provide better clinical outcomes for them: complete gene diagnostics, molecularly targeted anticancer treatment, immuno-oncology and immune cell therapy...
May 2018: Orvosi Hetilap
https://www.readbyqxmd.com/read/29753948/gender-related-differences-observed-among-immune-cells-in-synovial-fluid-in-knee-osteoarthritis
#3
E Kriegova, G Manukyan, Z Mikulkova, G Gabcova, M Kudelka, P Gajdos, J Gallo
OBJECTIVE: There is no existing comprehensive report on the cellular composition of synovial fluids (SFs) from knee osteoarthritis (OA). We therefore aimed to characterise the immune cell composition in SFs from knee OA and in subgroups according to gender. DESIGN: The immunophenotyping of monocyte/macrophage lineage cells, T and B cells, NK cells, neutrophils, dendritic and mast cells present in SFs from 53 patients (24 males/29 females) with knee OA was performed using 6-colour flow cytometry...
May 10, 2018: Osteoarthritis and Cartilage
https://www.readbyqxmd.com/read/29752628/correlation-between-heat-shock-proteins-adiponectin-and-t-lymphocyte-cytokine-expression-in-type-2-diabetics
#4
Fadia F Mahmoud, David Haines, Ali A Dashti, Sherief El-Shazly, Fawzia Al-Najjar
Type 2 diabetes mellitus (T2DM) features insulin resistance, hyperglycemia, dyslipidemia, overproduction of inflammatory cytokines, and systemic oxidative stress. Here, heat shock proteins Hsp70 and Hsp 90, adiponectin, and heme oxygenase-1 (HO-1, Hsp32) are profiled in peripheral blood mononuclear cells (PBMC) and serum from 25 T2DM patients and 25 healthy control subjects. Cells cultured with phorbol 12-myristate 13-acetate/ionomycin were evaluated by three-color flow cytometry for immunophenotypic biomarkers...
May 11, 2018: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/29751821/improved-gmp-compliant-approach-to-manipulate-lipoaspirates-to-cryopreserve-stromal-vascular-fraction-and-to-expand-adipose-stem-cells-in-xeno-free-media
#5
Francesco Agostini, Francesca Maria Rossi, Donatella Aldinucci, Monica Battiston, Elisabetta Lombardi, Stefania Zanolin, Samuele Massarut, Pier Camillo Parodi, Alessandro Da Ponte, Giovanni Tessitori, Barbara Pivetta, Cristina Durante, Mario Mazzucato
BACKGROUND: The stromal vascular fraction (SVF) derived from adipose tissue contains adipose-derived stromal/stem cells (ASC) and can be used for regenerative applications. Thus, a validated protocol for SVF isolation, freezing, and thawing is required to manage product administration. To comply with Good Manufacturing Practice (GMP), fetal bovine serum (FBS), used to expand ASC in vitro, could be replaced by growth factors from platelet concentrates. METHODS: Throughout each protocol, GMP-compliant reagents and devices were used...
May 11, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29739448/associations-between-endothelial-progenitor-cells-clinical-characteristics-and-coronary-restenosis-in-patients-undergoing-percutaneous-coronary-artery-intervention
#6
Fernando S Montenegro, Marcelo Correia, Fabiana Muccillo, Christina G Souza E Silva, Andrea De Lorenzo
OBJECTIVE: Endothelial progenitor cells (EPCs) are produced in the bone marrow and mobilized to the peripheral blood playing a key role in endothelial repair. The objective of this study was to evaluate circulating EPC before and after percutaneous coronary intervention (PCI) with stent implantation and their associations with coronary restenosis and adverse cardiovascular events. Venous blood was obtained before and the day after PCI. Quantification of total white blood count and identification of EPCs (CD45- CD34+ CD31+ CD133/2+ CD309+ ) through immunophenotyping by flow cytometry was performed...
May 8, 2018: BMC Research Notes
https://www.readbyqxmd.com/read/29725528/-blastoid-variant-of-burkitt-lymphoma-with-additional-partial-1q-tetrasomy
#7
Ahsan Siddiqi, Sheshadri Madhusudhana, Alexey Glazyrin
Burkitt lymphoma is cytogenetically characterized by t(8;14)(q24;q32) translocation, sometimes accompanied by additional cytogenetic abnormalities. These abnormalities usually result in more aggressive clinical presentation and morphology of the disease. The current report presens a case of Burkitt lymphoma with t(8;14)(q24;q32) accompanied by partial tetrasomy of chromosome 1(47,XY,+1,i(1)(q10),t(8;14)(q24;q32)[2]/46,XY[18]). The patient was a 59-year-old male who presented with abdominal pain, leukocytosis and tumor lysis syndrome...
May 2018: Molecular and Clinical Oncology
https://www.readbyqxmd.com/read/29712419/correlation-of-cell-surface-biomarker-expression-levels-with-adhesion-contact-angle-measured-by-lateral-microscopy
#8
Jenna A Walz, Charles R Mace
Immunophenotyping is typically achieved using flow cytometry, but any influence this biomarker may have on adhesion or surface recognition cannot be determined concurrently. In this manuscript, we demonstrate the utility of lateral microscopy for correlating cell surface biomarker expression levels with quantitative descriptions of cell morphology. With our imaging system, we observed single cells from two T cell lines and two B cell lines adhere to antibody-coated substrates and quantified this adhesion using contact angle measurements...
April 30, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29705861/the-prognostic-role-of-e2a-pbx1-expression-detected-by-real-time-quantitative-reverse-transcriptase-polymerase-chain-reaction-rq-pcr-in-b-cell-acute-lymphoblastic-leukemia-after-allogeneic-hematopoietic-stem-cell-transplantation
#9
Yan Hong, Xiaosu Zhao, Yazhen Qin, Songhai Zhou, Yingjun Chang, Yu Wang, Xiaohui Zhang, Lanping Xu, Xiaojun Huang
The E2A-PBX1 rearrangement is common in B cell acute lymphoblastic leukemia (B-ALL). However, whether this fusion gene can be used as a reliable marker for minimal residual disease (MRD) following allogeneic hematopoietic stem cell transplantation (allo-HSCT) remains unknown. In this study, clinical data were collected from 28 consecutive B-ALL patients who received allo-HSCT. Their MRD was evaluated by E2A-PBX1 and leukemia-associated immunophenotype (LAIP). The median follow-up was 374 days (55-2342 days)...
April 28, 2018: Annals of Hematology
https://www.readbyqxmd.com/read/29704326/polyclonal-b-cell-lymphocytosis-report-of-three-cases
#10
Natália Aydos Marcondes, Laiana Schneider, Bianca Michel Spindler, Flavo Beno Fernandes, Gustavo Adolpho Moreira Faulhaber
BACKGROUND: Persistent polyclonal B-cell lymphocytosis (PPBL) is a rare benign condition characterized by a polyclonal B-cell lymphocytosis with binucleated lymphocytes. We hereby report three cases of PPBL. METHODS: Flow cytometry immunophenotyping was performed in peripheral blood samples from three patients with clinical suspicion of lymphoproliferative disease. RESULTS: Case 1 was a female middle-aged smoker; case 2 was an elderly male; and case 3 was a non-smoker female...
April 27, 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29692781/association-of-decreased-percentage-of-v%C3%AE-2-v%C3%AE-9-%C3%AE-%C3%AE-t-cells-with-disease-severity-in-multiple-sclerosis
#11
Guzailiayi Maimaitijiang, Koji Shinoda, Yuri Nakamura, Katsuhisa Masaki, Takuya Matsushita, Noriko Isobe, Ryo Yamasaki, Yasunobu Yoshikai, Jun-Ichi Kira
We recently reported that deletion-type copy number variations of the T cell receptor (TCR) γ, α, and δ genes greatly enhanced susceptibility to multiple sclerosis (MS). However, the effect of abnormal TCR γδ gene rearrangement on MS pathogenesis remains unknown. In the present study, we aimed to clarify γδ TCR repertoire alterations and their relationship to clinical and immunological parameters in MS patients by comprehensive flow cytometric immunophenotyping. Peripheral blood mononuclear cells obtained from 30 untreated MS patients in remission and 23 age- and sex-matched healthy controls (HCs) were stained for surface markers and intracellular cytokines after stimulation with phorbol 12-myristate 13-acetate and ionomycin, and analyzed by flow cytometry...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29690889/effect-of-colorectal-cancer-derived-extracellular-vesicles-on-the-immunophenotype-and-cytokine-secretion-profile-of-monocytes-and-macrophages
#12
Ineta Popēna, Artūrs Ābols, Līga Saulīte, Kārlis Pleiko, Elīna Zandberga, Kaspars Jēkabsons, Edgars Endzeliņš, Alicia Llorente, Aija Linē, Una Riekstiņa
BACKGROUND: Macrophages are one of the most important players in the tumor microenvironment. The polarization status of tumor associated macrophages into a pro-inflammatory type M1 or anti-inflammatory type M2 may influence cancer progression and patient survival. Extracellular vesicles (EVs) are membrane-bound vesicles containing different biomolecules that are involved in cell to cell signal transfer. Accumulating evidence suggests that cancer-derived EVs are taken up by macrophages and modulate their phenotype and cytokine profile...
April 24, 2018: Cell Communication and Signaling: CCS
https://www.readbyqxmd.com/read/29687448/stem-cell-like-populations-and-immunoregulatory-molecules-in-periodontal-granulation-tissue
#13
D A Apatzidou, C Nile, A Bakopoulou, A Konstantinidis, D F Lappin
BACKGROUND AND OBJECTIVES: Determine the presence of mesenchymal stem cells (MSCs) in healthy periodontal tissue and periodontal granulation tissue (GT) and explore associations between immuno-regulatory molecules and selected subgingival microorganisms. MATERIAL AND METHODS: Mesenchymal stem cells were isolated, propagated and characterised by flow cytometry from a region of healthy gingival tissue and inflamed GT of 10 systemically healthy non-smokers with chronic periodontitis...
April 23, 2018: Journal of Periodontal Research
https://www.readbyqxmd.com/read/29680307/prognostic-value-of-cd95-active-caspase-3-and-bcl-2-expression-in-adult-patients-with-de-novo-acute-lymphoblastic-leukemia
#14
Laura Arcelia Montiel-Cervantes, Elba Reyes-Maldonado, Jaime Garcia-Chavez, Alvaro Hernandez-Caballero, Rubiraida Molina-Aguilar, Miriam America Garcia-Ruiz Esparza, Jorge Vela-Ojeda
BACKGROUND: Acute lymphoblastic leukemia is an aggressive malignant disease with high mortality rates in adults. AIM OF THE STUDY: The expression levels of CD95, active caspase-3, and Bcl-2 were determined in 111 patients with de novo acute lymphoblastic leukemia (ALL) and correlated with overall survival (OS) and disease-free survival (DFS). METHODS: The immunophenotyped ok leukemia and the expression of CD95, active caspase-3, and Bcl-2, were determined by flow cytometry...
April 18, 2018: Archives of Medical Research
https://www.readbyqxmd.com/read/29678226/multispectral-fluorescence-activated-cell-sorting-of-b-and-t-cell-subpopulations-from-equine-peripheral-blood
#15
Joy E Tomlinson, Bettina Wagner, M Julia B Felippe, Gerlinde R Van de Walle
Immune phenotyping of equine peripheral blood mononuclear cells (PBMC) is commonly described by single or double marker labeling, which limits complex phenotypic descriptions and subpopulation identification. Our objective was to develop a new multispectral flow cytometry protocol to identify and sort equine lymphocyte subpopulations using commercially available, pre-conjugated monoclonal antibodies to cell surface markers. Two clones of anti-equine CD8 (CVS8 and CVS21) were compared in combination with CD3...
May 2018: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/29676377/expect-the-unexpected-loss-of-surface-cd3-on-flow-cytometry-in-hepatosplenic-t-cell-lymphoma-an-eye-opener
#16
Richa Chauhan, Seema Tyagi, Sumeet Mirgh, Priyanka Mishra, Tulika Seth, Manoranjan Mahapatra, Haraprasad Pati, Renu Saxena
Hepatosplenic T-cell lymphoma (HSTCL) is a rare extranodal T-cell lymphoma that shows preferential sinusoidal infiltration of spleen and liver. It usually shows bright expression of surface CD3 (sCD3) with restriction for γδ-T cell receptors (TCR). We present a case of a 34-year-old male who presented with hepatosplenomegaly and B symptoms. His peripheral blood and bone marrow (BM) was involved by atypical lymphoid cells that were CD2+, CD7+, CD56+, cytoplasmic CD3+, and sCD3- on immunophenotyping by flow cytometry...
April 2018: Indian Journal of Pathology & Microbiology
https://www.readbyqxmd.com/read/29671598/impact-of-oxygen-concentration-on-metabolic-profile-of-human-placenta-derived-mesenchymal-stem-cells-as-determined-by-chemical-isotope-labeling-lc-ms
#17
Dan Wang, Deying Chen, Jiong Yu, Jingqi Liu, Xiaowei Shi, Yanni Sun, Qiaoling Pan, Xian Luo, Jinfeng Yang, Yang Li, Hongcui Cao, Liang Li, Lanjuan Li
The placenta resides in a physiologically low oxygen microenvironment of the body. Hypoxia induces a wide range of stem cell cellular activities. Here, we report a workflow for exploring the role of physiological (hypoxic, 5% oxygen) and original cell culture (normoxic, 21% oxygen) oxygen concentrations in regulating the metabolic status of human placenta-derived mesenchymal stem cells (hPMSCs). The general biological characteristics of hPMSCs were assessed via a variety of approaches such as cell counts, flow cytometry and differentiation study...
May 4, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/29669178/the-utility-of-multiparametric-flow-cytometry-in-the-detection-of-primary-effusion-lymphoma-pel
#18
Jacobo Galán, Isaac Martin, Itziar Carmona, José María Rodriguez-Barbero, Esperanza Cuadrado, Luis García-Alonso, José Antonio García-Vela
Primary effusion lymphoma (PEL) is a rare B cell lymphoproliferative disorder that arises predominantly in body cavities causing malignant effusions.The incidence of PEL is very low, accounting for approximately 4% of all HIV-associated non-Hodgkin lymphomas. PEL has also been described in elderly patients and after solid-organ transplantation. It is associated in all cases with human herpesvirus 8 (HHV8). We describe a case of PEL in a 88-year-old HIV-negative woman who presented with progressive dyspnea and moderate right-sided pleural effusion without significate lymphadenopathies or other effusions...
April 18, 2018: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/29666557/the-impact-of-delayed-sample-handling-and-type-of-anticoagulant-on-the-interpretation-of-dysplastic-signs-detected-by-flow-cytometry
#19
Bettina Kárai, Zsófia Miltényi, Lajos Gergely, Marianna Száraz-Széles, János Kappelmayer, Zsuzsanna Hevessy
Introduction: A growing body of evidence supports the usefulness of dysplastic signs detected by flow cytometry in the diagnosis of myelodysplastic syndromes (MDS). Our aim was to assess the impact of pre-analytical variables (delayed sample handling, type of anticoagulant, and different clones of antibody) in the interpretation of flow cytometric results. Material and methods: Bone marrow samples were labelled and analysed immediately after aspiration and on two consecutive days...
June 15, 2018: Biochemia Medica: časopis Hrvatskoga Društva Medicinskih Biokemičara
https://www.readbyqxmd.com/read/29665896/-a-modified-protocol-for-rapid-establishing-mouse-model-with-ph-positive-acute-lymphoblastic-leukemia
#20
Na Qi, Xue Wang, Qing-Yun Wu, Zhi-Ling Yan, Lin Wang, Chong Chen, Kai-Lin Xu
OBJECTIVE: To investigate a modified protocol of establishing mouse Ph+ ALL model so as to provide a more convenient and more powerful tool for Ph+ ALL studies. METHODS: Immature B cells from BALB/c mice were transfected with the Mig190 retrovirus and infused into irradiated syngeneic mice. The immunophenotype was identified by flow cytometry, the BCR-ABL was identified by RT-PCR and Western blot. Leukemia cells isolated from sick mice were re-infused into syngeneic mice without irradiation...
April 2018: Zhongguo Shi Yan Xue Ye Xue za Zhi
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