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https://www.readbyqxmd.com/read/29017490/germline-mutation-within-col2a1-associated-with-lethal-chondrodysplasia-in-a-polled-holstein-family
#1
Sina Reinartz, Hartmut Mohwinkel, Christian Sürie, Maren Hellige, Karsten Feige, Deborah Eikelberg, Andreas Beineke, Julia Metzger, Ottmar Distl
BACKGROUND: The bulldog calf syndrome is a lethal form of the inherited congenital chondrodysplasias. Among the progeny of the polled Holstein bull Energy P cases of lethal chondrodysplasia were observed. Pedigrees of the cases and the frequency of 3/8 cases among the offspring of Energy P at our teaching and experimental farm Ruthe (LuFG Ruthe) supported the assumption of a germline mutation with a mosaic of normal and defective sperm. RESULTS: All three malformed calves were examined using necropsy, histopathology and computed tomography scanning...
October 10, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28979232/beliefs-about-obedience-levels-in-studies-conducted-within-the-milgram-paradigm-better-than-average-effect-and-comparisons-of-typical-behaviors-by-residents-of-various-nations
#2
Tomasz Grzyb, Dariusz Dolinski
The article presents studies examining whether the better than average (BTA) effect appears in opinions regarding obedience of individuals participating in an experiment conducted in the Milgram paradigm. Participants are presented with a detailed description of the experiment, asked to declare at what moment an average participant would cease their participation in the study, and then asked to declare at what moment they themselves would quit the experiment. It turned out that the participants demonstrated a strong BTA effect...
2017: Frontiers in Psychology
https://www.readbyqxmd.com/read/28974708/embryological-evidence-substantiates-the-subcoxal-theory-on-the-origin-of-pleuron-in-insects
#3
Yuta Mashimo, Ryuichiro Machida
The lateral body plate pleuron is a significant structure in insects that contributes to the development and elaboration of wings and limbs (appendages). Although the pleuron is thought to originate from the proximal-most appendicular segment, the subcoxa, details remain unclear, and the morphological boundary between the dorsal body plate tergum and appendage (BTA) has not been clearly specified. Employing low-vacuum scanning electron microscopy (SEM) and the nano-suit method for SEM, we followed, in detail, the development of the thoracic segments of the two-spotted cricket Gryllus bimaculatus and succeeded in clearly defining the BTA...
October 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28966069/biotimer-assay-a-reliable-and-rapid-method-for-the-evaluation-of-central-venous-catheter-microbial-colonization
#4
Luigi Rosa, Antimo Cutone, Monica Coletti, Maria Stefania Lepanto, Mellani Scotti, Piera Valenti, Giammarco Raponi, Maria Cristina Ghezzi, Francesca Berlutti
Adherent bacteria and biofilm frequently colonize central venous catheters (CVCs). CVC colonization is correlated to infections and particularly to bloodstream ones. The classical microbiological methods to determine of CVC colonization are not fully reliable and are time-consuming. BioTimer Assay (BTA) is a biological method already used to count bacteria adherent to abiotic surfaces and biofilm without sample manipulation. BTA employs specific reagents whose color changed according to bacterial metabolism...
September 29, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28957330/application-of-single-step-genomic-blup-under-different-uncertain-paternity-scenarios-using-simulated-data
#5
Rafael Lara Tonussi, Rafael Medeiros de Oliveira Silva, Ana Fabrícia Braga Magalhães, Rafael Espigolan, Elisa Peripolli, Bianca Ferreira Olivieri, Fabieli Loise Braga Feitosa, Marcos Vinicíus Antunes Lemos, Mariana Piatto Berton, Hermenegildo Lucas Justino Chiaia, Angelica Simone Cravo Pereira, Raysildo Barbosa Lôbo, Luiz Antônio Framartino Bezerra, Cláudio de Ulhoa Magnabosco, Daniela Andressa Lino Lourenço, Ignácio Aguilar, Fernando Baldi
The objective of this study was to investigate the application of BLUP and single step genomic BLUP (ssGBLUP) models in different scenarios of paternity uncertainty with different strategies of scaling the G matrix to match the A22 matrix, using simulated data for beef cattle. Genotypes, pedigree, and phenotypes for age at first calving (AFC) and weight at 550 days (W550) were simulated using heritabilities based on real data (0.12 for AFC and 0.34 for W550). Paternity uncertainty scenarios using 0, 25, 50, 75, and 100% of multiple sires (MS) were studied...
2017: PloS One
https://www.readbyqxmd.com/read/28922408/the-role-of-the-pi3k-akt-signaling-pathway-in-the-developmental-competence-of-bovine-oocytes
#6
Gabriella Mamede Andrade, Juliano Coelho da Silveira, Claudia Perrini, Maite Del Collado, Samuel Gebremedhn, Dawit Tesfaye, Flávio Vieira Meirelles, Felipe Perecin
The ovarian follicle encloses oocytes in a microenvironment throughout their growth and acquisition of competence. Evidence suggests a dynamic interplay among follicular cells and oocytes, since they are constantly exchanging "messages". We dissected bovine ovarian follicles and recovered follicular cells (FCs-granulosa and cumulus cells) and cumulus-oocyte complexes (COCs) to investigate whether the PI3K-Akt signaling pathway impacted oocyte quality. Following follicle rupture, COCs were individually selected for in vitro cultures to track the follicular cells based on oocyte competence to reach the blastocyst stage after parthenogenetic activation...
2017: PloS One
https://www.readbyqxmd.com/read/28903352/analysis-of-bos-taurus-and-sus-scrofa-x-and-y-chromosome-transcriptome-highlights-reproductive-driver-genes
#7
Faheem Ahmed Khan, Hui Liu, Hao Zhou, Kai Wang, Muhammad Tahir Ul Qamar, Nuruliarizki Shinta Pandupuspitasari, Zhang Shujun
The biology of sperm, its capability of fertilizing an egg and its role in sex ratio are the major biological questions in reproductive biology. To answer these question we integrated X and Y chromosome transcriptome across different species: Bos taurus and Sus scrofa and identified reproductive driver genes based on Weighted Gene Co-Expression Network Analysis (WGCNA) algorithm. Our strategy resulted in 11007 and 10445 unique genes consisting of 9 and 11 reproductive modules in Bos taurus and Sus scrofa, respectively...
August 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28902440/real-time-control-of-the-enantioselectivity-of-a-supramolecular-catalyst-allows-selecting-the-configuration-of-consecutively-formed-stereogenic-centers
#8
Jeremy M Zimbron, Xavier Caumes, Yan Li, Christophe M Thomas, Matthieu Raynal, Laurent Bouteiller
The enantiomeric state of a supramolecular copper catalyst can be switched in situ in ca. five seconds. The dynamic property of the catalyst is provided by the non-covalent nature of the helical assemblies supporting the copper centers. These assemblies are formed by mixing an achiral benzene-1,3,5-tricarboxamide (BTA) phosphine ligand (for copper coordination) and both enantiomers of a chiral phosphine-free BTA co-monomer (for chirality amplification). The enantioselectivity of the hydrosilylation reaction is fixed by the BTA enantiomer in excess, which can be altered by simple BTA addition...
September 13, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28891795/-intravesical-botulinum-toxin-under-local-anestesia-as-ambulatory-procedure
#9
José Javier Fabuel Alcañiz, Laura Martínez Arcos, Miguel Jimenez Cidre, Francisco Javier Burgos Revilla
Bladder hyperactivity is described as the presence of "voiding urgency, generally associated with increased daytime frequency and nocturia, with or without urinary incontinence, in the absence of urinary tract infection or other obvious pathology". Onabotulinum toxin A (BTA) is a recommendable therapeutic option in case of failure, contraindication or refusal of the conservative therapy or other non-pharmacological therapies. The injection of BTA in the detrusor has been performed under local, regional or general anesthesia either in the conventional or major ambulatory surgery operative room or in the cystoscopy room...
September 2017: Archivos Españoles de Urología
https://www.readbyqxmd.com/read/28870843/phosphatidylserine-a-cancer-cell-targeting-biomarker
#10
REVIEW
Bhupender Sharma, Shamsher S Kanwar
Cancer is a leading cause of mortality and morbidity globally. Many prominent cancer-associated molecules have been identified over the recent years which include EGFR, CD44, TGFbRII, HER2, miR-497, NMP22, BTA, Fibrin/FDP etc. These biomarkers are often used for screening, detection, diagnosis, prognosis, prediction and monitoring of cancer development. Phosphatidylserine (PS) is an essential component in all human cells which is present on the inner leaflet of the cell membrane. The oxidative stress causes exposure of PS on the surface of the vascular endothelium in the cancer cells (lung, breast, pancreatic, bladder, skin, brain metastasis, rectal adenocarcinoma etc...
September 1, 2017: Seminars in Cancer Biology
https://www.readbyqxmd.com/read/28865853/fine-mapping-of-bta17-using-imputed-sequences-for-associations-with-de-novo-synthesized-fatty-acids-in-bovine-milk
#11
S I Duchemin, H Bovenhuis, H-J Megens, J A M Van Arendonk, M H P W Visker
The aim of this study was to fine-map a genomic region associated with milk fatty acids (FA) on Bos taurus autosome (BTA) 17. This genomic region has been discovered with 50,000 (50k) single nucleotide polymorphisms (SNP) imputed to 777,000 (777k) SNP. In this study, high-density genotypes were imputed to whole-genome sequences level to identify candidate gene(s) associated with milk FA composition on BTA17. Phenotypes and genotypes were available for 1,640 cows sampled in winter, and for 1,581 cows sampled in summer...
November 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28859894/the-prevalence-and-impact-of-urinary-marker-testing-in-patients-with-bladder-cancer
#12
Vikram M Narayan, Oluwakayode Adejoro, Ian Schwartz, Matthew Ziegelmann, Sean Elliott, Badrinath R Konety
PURPOSE: Novel urinary tumor markers for bladder cancer may permit early detection and improved oncologic outcomes, but data regarding their utilization is limited. We sought to identify trends in utilization of urinary markers and long-term outcomes of urinary tumor marker use in patients with bladder cancer. MATERIALS AND METHODS: Data from the Surveillance, Epidemiology, and End Results-Medicare (SEER-Medicare) database from 2001 to 2011 were used to identify a cohort of 64,450 patients with bladder cancer who underwent urinary marker testing (UMT) with UroVysion FISH, NMP22, or the BTA Stat tests...
August 28, 2017: Journal of Urology
https://www.readbyqxmd.com/read/28858275/-botulinus-toxin-in-complex-treatment-of-myofacial-pain-syndrome
#13
S N Fedotov, M A Gerasimova, S D Shorokhov, A I Tischenko
The aim of the study was to assess the efficacy of type A Botulinus toxin (BTA) in pain release by TMJ functional pain disorders. The study included 211 patients with TMJ functional pain disorder (20.4% males and 79.6% females; mean age 45.3 years). The patients underwent clinical examination and bioelectric activity assessment of masticatory muscles by electromyography (EMG). EMG specters of 20 healthy volunteers with intact dental arches served as a control. After examination BTA was injected in muscular pain trigger points...
2017: Stomatologii︠a︡
https://www.readbyqxmd.com/read/28844020/biocomputational-identification-and-validation-of-novel-micrornas-predicted-from-bubaline-whole-genome-shotgun-sequences
#14
H K Manku, J K Dhanoa, S Kaur, J S Arora, C S Mukhopadhyay
MicroRNAs (miRNAs) are small (19-25 base long), non-coding RNAs that regulate post-transcriptional gene expression by cleaving targeted mRNAs in several eukaryotes. The miRNAs play vital roles in multiple biological and metabolic processes, including developmental timing, signal transduction, cell maintenance and differentiation, diseases and cancers. Experimental identification of microRNAs is expensive and lab-intensive. Alternatively, computational approaches for predicting putative miRNAs from genomic or exomic sequences rely on features of miRNAs viz...
August 14, 2017: Computational Biology and Chemistry
https://www.readbyqxmd.com/read/28843680/genome-wide-association-and-pathway-based-analysis-using-latent-variables-related-to-milk-protein-composition-and-cheesemaking-traits-in-dairy-cattle
#15
Christos Dadousis, Sara Pegolo, Guilherme J M Rosa, Giovanni Bittante, Alessio Cecchinato
The aim of this study was to perform genome-wide associations (GWAS) and gene-set enrichment analyses with protein composition and cheesemaking-related latent variables (factors; F) in a cohort of 1,011 Italian Brown Swiss cows. Factor analysis was applied to identify latent structures of 26 phenotypes related to bovine milk quantity and quality, protein fractions [αS1-, αS2-, β-, and κ-casein (CN), β-lactoglobulin, and α-lactalbumin (α-LA)], coagulation and curd firming at time t (CFt) measures, and cheese properties [cheese yield (%CY) and nutrients recovery in the curd] of individual cows...
November 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28840969/intraprostatic-botulinum-toxin-type-a-injection-in-patients-with-benign-prostatic-hyperplasia-and-unsatisfactory-response-to-medical-therapy-a-randomized-double-blind-controlled-trial-using-urodynamic-evaluation
#16
Angelo Totaro, Francesco Pinto, Dario Pugliese, Matteo Vittori, Marco Racioppi, Nazario Foschi, Pier Francesco Bassi, Emilio Sacco
AIMS: The injection of botulinum neurotoxin A (BTA) into the prostate represents a minimally invasive treatment in patients with lower urinary tract symptoms (LUTS) associated to benign prostatic hyperplasia (BPH). We evaluated the effectiveness of BTA in treating patients with BPH unresponsive to combined medical therapy (CMT), using urodynamic investigations. METHODS: This is a randomized, placebo-controlled, double blind trial. Twenty consecutive patients were randomly assigned to receive intraprostatic BTA injection (n = 10) or saline solution (SS) (n = 10)...
August 25, 2017: Neurourology and Urodynamics
https://www.readbyqxmd.com/read/28829076/ultrathin-polydopamine-film-coated-gold-nanoparticles-a-sensitive-uniform-and-stable-shiners-substrate-for-detection-of-benzotriazole
#17
Weichun Ye, Hong Huang, Weiwei Yang, Xiang Wang, Cuiling Ren, Qiushi Hu, Yumin Li, Bin Ren
In shell-isolated nanoparticle (NP)-enhanced Raman spectroscopy (SHINERS), traditional metal oxide-based shells have inferior chemical inertness, they require strict preparation conditions, and lack specific groups, which lead to their poor selectivity toward target molecules. In this study, ultrathin and compact gold (Au)@polydopamine (PDA) SHINERS NPs were successfully fabricated by a simple self-polymerization technique. High wrapping tendency of PDA, a multifunctional biopolymer, favored the fabrication process...
August 22, 2017: Analyst
https://www.readbyqxmd.com/read/28812212/obesity-treatment-with-botulinum-toxin-a-is-not-effective-a-systematic-review-and-meta-analysis
#18
REVIEW
Fabio Bustamante, Vitor Ottoboni Brunaldi, Wanderley Marques Bernardo, Diogo Turiani H de Moura, Eduardo Turiani H de Moura, Manoel Galvão, Marco Aurélio Santo, Eduardo Guimarães H de Moura
The effectiveness of gastric injections of botulinum toxin-A (BTA) as primary treatment for obesity is not well known since results in literature are discrepant. Hence, we aimed to systematically review and meta-analyze the available data to assess the real effect of BTA therapy. We searched MEDLINE, Embase, Cochrane, SCOPUS, EBSCO, LILACS, and BVS. We considered eligible only randomized controlled trials enrolling obese patients comparing BTA versus saline injections. Our initial search identified 8811 records...
August 15, 2017: Obesity Surgery
https://www.readbyqxmd.com/read/28782787/in-situ-synthesized-3d-metal-organic-frameworks-mofs-constructed-from-transition-metal-cations-and-tetrazole-derivatives-a-family-of-insensitive-energetic-materials
#19
Yuangang Xu, Wei Liu, Dongxue Li, Houhe Chen, Ming Lu
The combination of the hydrothermal method with in situ synthesis has been successfully employed to prepare a family of tetrazole-based energetic metal-organic frameworks (EMOFs) ([Ag(Mtta)]n, 1; [Cd5(Mtta)9]n, 2; [Pb3(bta)2(O)2(H2O)]n, 3; and [Pb(tztr)2(H2O)]n, 4) through [2 + 3] cycloaddition of azide anions and nitrile groups. All the synthesized EMOFs were characterized by single crystal X-ray diffraction, IR spectroscopy, elemental analysis (EA), different scanning calorimetry (DSC), and thermogravimetry (TG)...
August 22, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/28776223/differential-expression-of-micrornas-associated-with-thermal-stress-in-frieswal-bos-taurus-x-bos-indicus-crossbred-dairy-cattle
#20
Gyanendra Singh Sengar, Rajib Deb, Umesh Singh, T V Raja, Rajiv Kant, Basavraj Sajjanar, Rani Alex, R R Alyethodi, Ashish Kumar, Sushil Kumar, Rani Singh, Subhash J Jakhesara, C G Joshi
Environmental temperature is one of the important abiotic factors that influence the normal physiological function and productive performance of dairy cattle. Temperature stress evokes complex responses that are essential for safeguarding of cellular integrity and animal health. Post-transcriptional regulation of gene expression by miRNA plays a key role cellular stress responses. The present study investigated the differential expression of miRNA in Frieswal (Holstein Friesian × Sahiwal) crossbred dairy cattle that are distinctly adapted to environmental temperature stress as they were evolved by using the temperate dairy breed Holstein Friesian...
August 3, 2017: Cell Stress & Chaperones
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