keyword
MENU ▼
Read by QxMD icon Read
search

Metabonomics

keyword
https://www.readbyqxmd.com/read/27881338/-plasma-metabonomics-of-guifu-dihuang-wan-in-the-treatment-of-yang-deficiency
#1
Ya Xiao, Yuan Jing, Jie-Yu Chen, Fei Li, Jing-Ru Cheng, Jian-Lu Bi, Ren Luo, Xiao-Shan Zhao
OBJECTIVE: To assess the effect of Guifu Dihuang Wan (GFDHW) in the treatment of yang deficiency and explore the underlying molecular mechanism. METHODS: Sixty-two participants without diseases were randomized into control group (n=31) and experimental group (n=31) and were given lifestyle intervention additional GFDHW treatment for a month. NMR technology was used for metabonomics analysis. RESULTS: Intervention with GFDHW resulted in significantly decreased conversion scores of yang deficiency in the experimental group compared with the control group (P<0...
November 20, 2016: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/27859443/metabolic-characterization-of-the-early-stage-of-hepatic-fibrosis-in-rat-using-gc-tof-ms-and-multivariate-data-analyses
#2
Hui Jiang, Jun-Mei Song, Peng-Fei Gao, Xiu-Juan Qin, Shuang-Zhi Xu, Jia-Fu Zhang
The aim of this study was to explore the changes in the urine metabolic spectrum in rats with the early stage of liver fibrosis using gas chromatography-time of flight/mass spectrometry (GC-TOF/MS), try to search for potential biomarkers and elucidate the probably metabonomic pathogenesis. The early stage of liver fibrosis was established with a single subcutaneously injection carbon tetrachloride (CCl4 ) twice each week for 4 weeks continuously. At the end of the experiment, GC-TOF/MS technology with multivariate statistical approaches such as principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) was used to analyze the changes in the metabolic spectrum trajectory and identify potential biomarkers...
November 17, 2016: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/27856104/the-investigation-of-anti-inflammatory-activity-of-yi-guanjian-decoction-by-serum-metabonomics-approach
#3
Sufang Shui, Xiaorong Cai, Rongqing Huang, Bingkun Xiao, Jianyun Yang
Yi Guanjian (YGJ), one of the Chinese herbal medicines most commonly used in western countries, reported to possess significant anti-inflammatary effects that inhibit the process of inflammation. However, the mechanisms underlying its anti-inflammation effects remain largely unresolved. This study was aimed to investigate the anti-inflammatory activity of YGJ and to explore its potential anti-inflammatory mechanisms by serum metabonomics approach. An xylene-induced mouse right-ear-edema model was used as an inflammatory response in vivo model...
January 30, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/27845778/identification-of-sex-specific-urinary-biomarkers-for-major-depressive-disorder-by-combined-application-of-nmr-and-gc-ms-based-metabonomics
#4
P Zheng, J-J Chen, C-J Zhou, L Zeng, K-W Li, L Sun, M-L Liu, D Zhu, Z-H Liang, P Xie
Women are more vulnerable to major depressive disorder (MDD) than men. However, molecular biomarkers of sex differences are limited. Here we combined gas chromatography-mass spectrometry (GC-MS)- and nuclear magnetic resonance (NMR)-based metabonomics to investigate sex differences of urinary metabolite markers in MDD, and further explore their potential of diagnosing MDD. Consequently, the metabolite signatures of women and men MDD subjects were significantly different from of that in their respective healthy controls (HCs)...
November 15, 2016: Translational Psychiatry
https://www.readbyqxmd.com/read/27845029/establishment-and-optimization-of-nmr-based-cell-metabonomics-study-protocols-for-neonatal-sprague-dawley-rat-cardiomyocytes
#5
Ming Zhang, Bo Sun, Qi Zhang, Rong Gao, Qiao Liu, Fangting Dong, Haiqin Fang, Shuangqing Peng, Famei Li, Xianzhong Yan
A quenching, harvesting, and extraction protocol was optimized for cardiomyocytes NMR metabonomics analysis in this study. Trypsin treatment and direct scraping cells in acetonitrile were compared for sample harvesting. The results showed trypsin treatment cause normalized concentration increasing of phosphocholine and metabolites leakage, since the trypsin-induced membrane broken and long term harvesting procedures. Then the intracellular metabolite extraction efficiency of methanol and acetonitrile were compared...
November 11, 2016: Analytical Biochemistry
https://www.readbyqxmd.com/read/27823631/pharmacokinetics-and-pharmacodynamics-study-of-rhein-treating-renal-fibrosis-based-on-metabonomics-approach
#6
Hao Sun, Guangwen Luo, Zheng Xiang, Xiaojun Cai, Dahui Chen
BACKGROUND: The selection of effect indicators in the pharmacokinetic/ pharmacodynamic study of complex diseases to describe the relationship between plasma concentration and effect indicators is difficult. PURPOSE: Three effect indicators of renal fibrosis were successfully determined. The relationship between pharmacokinetics and pharmacodynamics of rhein in rhubarb was elucidated. STUDY DESIGN: The study was a metabolomics analysis of rat plasma and pharmacokinetics/ pharmacodynamics of rhein...
December 1, 2016: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/27810280/-1-h-nmr-based-metabonomic-study-on-the-effects-of-epimedium-on-glucocorticoid-induced-osteoporosis
#7
Sina Pan, Ali Chen, Zhihui Han, Yaling Wang, Xin Lu, Yongxia Yang
Glucocorticoids are widely used in clinical practice for the treatment of many immune-mediated and inflammatory diseases, and glucocorticoid-induced osteoporosis (GIO) is the most common type of secondary osteoporosis. Epimedium is one of the most commonly used traditional Chinese medicines for treating osteoporosis. In the present study, we systematically analysed the metabonomic characteristics of GIO model rats and elucidated the therapeutic effect of Epimedium by using a (1)H NMR-based metabonomic approach in conjunction with multivariate data analysis...
October 14, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/27769393/simultaneous-profiling-of-eicosanoid-metabolome-in-plasma-by-uplc-ms-ms-method-application-to-identify-potential-makers-for-rheumatoid-arthritis
#8
Nannan Wang, Ronghua Dai, Weihui Wang, Yan Peng, Xiaoning Zhao, Kaishun Bi
To evaluate the potential relationship between rheumatoid arthritis and arachidonic acid (AA) metabonomics via cyclooxygenase (COX) and lipoxygenase (LOX) pathways, a UPLC-MS/MS method has been developed and validated for simultaneous and quantitative profiling of eicosanoid metabolome in rat plasma. The analytes were extracted from plasma samples by protein precipitation procedure, and then separated on a Shim-pack XR-ODS column with mobile phase A (0.05% formic acid in water, pH=3.3 adjusted with dilute ammonium hydroxide) and mobile phase B [methanol: acetonitrile (20:80, v/v)]...
December 1, 2016: Talanta
https://www.readbyqxmd.com/read/27746335/nmr-based-metabonomics-study-on-the-effect-of-gancao-in-the-attenuation-of-toxicity-in-rats-induced-by-fuzi
#9
Bo Sun, Xubin Wang, Ruili Cao, Qi Zhang, Qiao Liu, Meifeng Xu, Ming Zhang, Xiangbo Du, Fangting Dong, Xianzhong Yan
ETHNOPHARMACOLOGICAL RELEVANCE: Fuzi, the processed lateral root of Aconitum carmichaelii Debeaux, is a traditional Chinese medicine used for its analgesic, antipyretic, anti-rheumatoid arthritis and anti-inflammation effects; however, it is also well known for its toxicity. Gancao, the root of Glycyrrhiza uralensis Fisch., is often used concurrently with Fuzi to alleviate its toxicity. However, the mechanism of detoxication is still not well clear. AIM OF THE STUDY: In this study, the effect of Gancao on the metabolic changes induced by Fuzi was investigated by NMR-based metabonomic approaches...
October 13, 2016: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/27723511/perturbation-of-metabonome-of-embryo-larvae-zebrafish-after-exposure-to-fipronil
#10
Lu Yan, Chenxue Gong, Xiaofeng Zhang, Quan Zhang, Meirong Zhao, Cui Wang
The escalating demand for fipronil by the increasing insects' resistance to synthetic pyrethroids placed a burden on aquatic vertebrates. Although awareness regarding the toxicity of fipronil to fish is arising, the integral alteration caused by fipronil remains unexplored. Here, we investigated on the development toxicity of fipronil and the metabolic physiology perturbation at 120h post fertilization through GC-MS metabolomics on zebrafish embryo. We observed that fipronil dose-dependently induced malformations including uninflated swim bladder and bent spine...
December 2016: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/27721983/decreased-glutathione-biosynthesis-contributes-to-egfr-t790m-driven-erlotinib-resistance-in-non-small-cell-lung-cancer
#11
Hongde Li, William Stokes, Emily Chater, Rajat Roy, Elza de Bruin, Yili Hu, Zhigang Liu, Egbert F Smit, Guus Jje Heynen, Julian Downward, Michael J Seckl, Yulan Wang, Huiru Tang, Olivier E Pardo
Epidermal growth factor receptor (EGFR) inhibitors such as erlotinib are novel effective agents in the treatment of EGFR-driven lung cancer, but their clinical impact is often impaired by acquired drug resistance through the secondary T790M EGFR mutation. To overcome this problem, we analysed the metabonomic differences between two independent pairs of erlotinib-sensitive/resistant cells and discovered that glutathione (GSH) levels were significantly reduced in T790M EGFR cells. We also found that increasing GSH levels in erlotinib-resistant cells re-sensitised them, whereas reducing GSH levels in erlotinib-sensitive cells made them resistant...
2016: Cell Discovery
https://www.readbyqxmd.com/read/27697573/nmr-based-metabonomics-and-correlation-analysis-reveal-potential-biomarkers-associated-with-chronic-atrophic-gastritis
#12
Jiajia Cui, Yuetao Liu, Yinghuan Hu, Jiayu Tong, Aiping Li, Tingli Qu, Xuemei Qin, Guanhua Du
Chronic atrophic gastritis (CAG) is one of the most important pre-cancerous states with a high prevalence. Exploring of the underlying mechanism and potential biomarkers is of significant importance for CAG. In the present work, (1)H NMR-based metabonomics with correlative analysis was performed to analyze the metabolic features of CAG. 19 plasma metabolites and 18 urine metabolites were enrolled to construct the circulatory and excretory metabolome of CAG, which was in response to alterations of energy metabolism, inflammation, immune dysfunction, as well as oxidative stress...
September 28, 2016: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/27696270/new-technologies-new-insights-into-the-pathogenesis-of-hepatic-encephalopathy
#13
Luisa Baker, Bernard Lanz, Fausto Andreola, Javier Ampuero, Anisha Wijeyesekera, Elaine Holmes, Nicolaas Deutz
Hepatic encephalopathy (HE) is a neuropsychiatric syndrome which frequently accompanies acute or chronic liver disease. It is characterized by a variety of symptoms of different severity such as cognitive deficits and impaired motor functions. Currently, HE is seen as a consequence of a low grade cerebral oedema associated with the formation of cerebral oxidative stress and deranged cerebral oscillatory networks. However, the pathogenesis of HE is still incompletely understood as liver dysfunction triggers exceptionally complex metabolic derangements in the body which need to be investigated by appropriate technologies...
September 30, 2016: Metabolic Brain Disease
https://www.readbyqxmd.com/read/27670847/staging-research-of-human-lung-cancer-tissues-by-high-resolution-magic-angle-spinning-proton-nuclear-magnetic-resonance-spectroscopy-hrmas-1-h-nmr-and-multivariate-data-analysis
#14
Wenxue Chen, Shaohua Lu, Guifang Wang, Fener Chen, Chunxue Bai
AIM: High-resolution magic-angle spinning proton nuclear magnetic resonance (HRMAS (1) H NMR) spectroscopy technique was employed to analyze the metabonomic characterizations of lung cancer tissues in hope to identify potential diagnostic biomarkers for malignancy detection and staging research of lung tissues. METHODS: HRMAS (1) H NMR spectroscopy technique can rapidly provide important information for accurate diagnosis and staging of cancer tissues owing to its noninvasive nature and limited requirement for the samples, and thus has been acknowledged as an excellent tool to investigate tissue metabolism and provide a more realistic insight into the metabonomics of tissues when combined with multivariate data analysis (MVDA) such as component analysis and orthogonal partial least squares-discriminant analysis in particular...
September 27, 2016: Asia-Pacific Journal of Clinical Oncology
https://www.readbyqxmd.com/read/27664398/efficacy-and-role-of-inulin-in-mitigation-of-enteric-sulfur-containing-odor-in-pigs
#15
Yuan-Fan Deng, Yuan-Yuan Liu, Yi-Tao Zhang, Yan Wang, Juan-Boo Liang, Vincenzo Tufarelli, Vito Laudadio, Xin-Di Liao
BACKGROUND: The efficacy and role of inulin in the mitigation of enteric sulfur-containing odor gases hydrogen sulfide (H2 S) and methyl mercaptan (CH3 SH) in pigs were examined in this study. Twelve Duroc × Landrace × Yorkshire male finisher pigs (60.7 ± 1.9 kg), housed individually in open-circuit respiration chambers, were randomly assigned to two dietary groups, namely basal diet (control) and basal diet supplemented with 1% (w/w) inulin. At the end of the 45 day experiment, pigs were slaughtered and volatile fatty acid (VFA) concentration, sulfate radical (SO4(2)(-) ) concentration, population of sulfate-reducing bacteria (SRB) and expression of methionine gamma-lyase (MGL) gene were determined in contents from the caecum, colon (two segments) and rectum...
September 24, 2016: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/27660611/from-metabonomics-to-pharmacometabonomics-the-role-of-metabolic-profiling-in-personalized-medicine
#16
REVIEW
Jeremy R Everett
Variable patient responses to drugs are a key issue for medicine and for drug discovery and development. Personalized medicine, that is the selection of medicines for subgroups of patients so as to maximize drug efficacy and minimize toxicity, is a key goal of twenty-first century healthcare. Currently, most personalized medicine paradigms rely on clinical judgment based on the patient's history, and on the analysis of the patients' genome to predict drug effects i.e., pharmacogenomics. However, variability in patient responses to drugs is dependent upon many environmental factors to which human genomics is essentially blind...
2016: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/27633843/global-metabolic-stress-of-isoeffort-continuous-and-high-intensity-interval-aerobic-exercise-a-comparative-1-h-nmr-metabonomic-study
#17
Andreas Zafeiridis, Anastasia Chrysovalantou Chatziioannou, Haralambos Sarivasiliou, Antonios Kyparos, Michalis G Nikolaidis, Ioannis S Vrabas, Alexandros Pechlivanis, Panagiotis Zoumpoulakis, Constantinos Baskakis, Konstantina Dipla, Georgios A Theodoridis
The overall metabolic/energetic stress that occurs during an acute bout of exercise is proposed to be the main driving force for long-term training adaptations. Continuous and high-intensity interval exercise protocols (HIIE) are currently prescribed to acquire the muscular and metabolic benefits of aerobic training. We applied (1)H NMR-based metabonomics to compare the overall metabolic perturbation and activation of individual bioenergetic pathways of three popular aerobic exercises matched for effort/strain...
October 4, 2016: Journal of Proteome Research
https://www.readbyqxmd.com/read/27610185/a-novel-method-for-evaluating-the-cardiotoxicity-of-traditional-chinese-medicine-compatibility-by-using-support-vector-machine-model-combined-with-metabonomics
#18
Yubo Li, Haonan Zhou, Jiabin Xie, Mayassa Salum Ally, Zhiguo Hou, Yanyan Xu, Yanjun Zhang
Traditional biochemical and histopathological tests have been used to evaluate the safety of traditional Chinese medicine (TCM) compatibility for a long time. But these methods lack high sensitivity and specificity. In the previous study, we have found ten biomarkers related to cardiotoxicity and established a support vector machine (SVM) prediction model. Results showed a good sensitivity and specificity. Therefore, in this study, we used SVM model combined with metabonomics UPLC/Q-TOF-MS technology to build a rapid and sensitivity and specificity method to predict the cardiotoxicity of TCM compatibility...
2016: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/27598867/plasma-metabolic-profiling-analysis-of-toxicity-induced-by-brodifacoum-using-metabonomics-coupled-with-multivariate-data-analysis
#19
Hui Yan, Zheng Qiao, Baohua Shen, Ping Xiang, Min Shen
Brodifacoum is one of the most widely used rodenticides for rodent control and eradication; however, human and animal poisoning due to primary and secondary exposure has been reported since its development. Although numerous studies have described brodifacoum induced toxicity, the precise mechanism still needs to be explored. Gas chromatography mass spectrometry (GC-MS) coupled with an ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was applied to characterize the metabolic profile of brodifacoum induced toxicity and discover potential biomarkers in rat plasma...
October 2016: Forensic Science International
https://www.readbyqxmd.com/read/27545408/metabolic-mechanism-of-a-polysaccharide-from-schisandra-chinensis-to-relieve-chronic-fatigue-syndrome
#20
Aiping Chi, Yang Zhang, Yijiang Kang, Zhimei Shen
Schisandra chinensis fruits are a famous traditional Chinese medicine to treat all kinds of fatigue. This study aimed to investigate the therapeutic effect and metabolic mechanism of a polysaccharide (SCP) from Schisandra chinensis fruits on chronic fatigue syndrome (CFS). SCP was isolated and the physicochemical properties were analyzed. A CFS model of rats was established and the urinary metabonomic studies were performed using gas chromatography time-of-flight mass spectrometry (GC-TOF-MS) in combination with multivariate statistical analysis...
August 18, 2016: International Journal of Biological Macromolecules
keyword
keyword
8337
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"