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Xiaowei Lou, Yangfang Ye, Ying Wang, Yangying Sun, Daodong Pan, Jinxuan Cao
The effect of high-pressure (HP) (0.1, 150 and 300MPa, 15min) on taste profiles of vinasse-cured ducks was investigated; the metabolite profiles were determined using 1H NMR. HP at 150MPa increased the taste intensity of products compared with the controls, while HP at 300MPa did not further improve their taste compared with 150MPa treated samples. The metabonome of vinasse-cured ducks was dominated by 27 metabolites. HP increased amino acids, glucose, alkaloids and organic acids, but decreased inosine monophosphate and its derivatives, compared with the controls...
March 2018: Food Research International
Lei Zhu, Zhi Jun Zhao, Xiao Bin Ren, Qiang Li, Hua Ding, Zhou Sun, Qing Jun Kao, Li Hua Wang
The molecular pathogenesis of T-2 toxin-induced cartilage destruction has not been fully unraveled yet. The aim of this study was to detect changes in serum metabolites in a rat anomaly model with articular cartilage destruction. Thirty healthy male Wistar rats were fed a diet containing T-2 toxin (300 ng/kg chow) for 3 months. Histopathological changes in femorotibial cartilage were characterized in terms of chondrocyte degeneration/necrosis and superficial cartilage defect, and the endogenous metabolite profile of serum was determined by UPLC/Q-TOF MS...
January 2018: Biomedical and Environmental Sciences: BES
Andrea Dória Batista, Carlos Jonnatan Pimentel Barros, Tássia Brena Barroso Carneiro Costa, Michele Maria Gonçalves de Godoy, Ronaldo Dionísio Silva, Joelma Carvalho Santos, Mariana Montenegro de Melo Lira, Norma Thomé Jucá, Edmundo Pessoa de Almeida Lopes, Ricardo Oliveira Silva
AIM: To develop metabonomic models (MMs), using 1 H nuclear magnetic resonance (NMR) spectra of serum, to predict significant liver fibrosis (SF: Metavir ≥ F2), advanced liver fibrosis (AF: METAVIR ≥ F3) and cirrhosis (C: METAVIR = F4 or clinical cirrhosis) in chronic hepatitis C (CHC) patients. Additionally, to compare the accuracy of the MMs with the aspartate aminotransferase to platelet ratio index (APRI) and fibrosis index based on four factors (FIB-4). METHODS: Sixty-nine patients who had undergone biopsy in the previous 12 mo or had clinical cirrhosis were included...
January 27, 2018: World Journal of Hepatology
Kuo Gao, Ran Yang, Jian Zhang, Zhiyong Wang, Caixia Jia, Feilong Zhang, Shaojing Li, Jinping Wang, Ghulam Murtaza, Hua Xie, Huihui Zhao, Wei Wang, Jianxin Chen
Qijian mixture, a new traditional Chinese medicine (TCM) formula comprising of Astragalus membranaceus, Ramulus euonymi, Coptis chinensis and Pueraria lobata, was designed to ameliorate the type 2 diabetes (T2D), and its safety and efficacy were evaluated in the research by metabonomics, gut microbiota and system pharmacology. To study the hypoglycemic effect of Qijian mixture, male KKay mice (28-30 g, 8-9 week) and C57/BL6 mice (18-19 g, 8-9 week) were used. Thirty KKay diabetic mice were randomly distributed into 5 groups, abbreviated as Model group (Model), Low Qijian Mixture group (QJM(L)), High Qijian Mixture group (QJM(H)), Chinese Medicine (Gegen Qinlian Decoction) Positive group (GGQL), and Western Medicine (Metformin hydrochloride) Positive group (Metformin)...
January 29, 2018: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
Lin Sun, Biao Xie, Qiuju Zhang, Yupeng Wang, Xinyu Wang, Bing Gao, Meina Liu, Maoqing Wang
Background: In children with Henoch-Schonlein purpura (HSP), the severity of Henoch-Schonlein purpura nephritis (HSPN) is considered responsible for the prognosis of HSP. The pathological process from HSP to HSPN is not clear yet and current diagnostic tools have shortcomings in accurate diagnosis of HSPN. This study aims to assess clinical characteristics of HSP and HSPN, to identify metabolic perturbations involved in HSP progress, and to combine metabolic biomarkers and clinical features into a better prediction for HSPN...
December 26, 2017: Oncotarget
Kelei Li, Xiaotian Zhang, Gong Chen, Lijun Pei, Hailong Xiao, Jiajing Jiang, Jiaomei Li, Xiaoying Zheng, Duo Li
The aim of the present study was to evaluate the association of fatty acids and lipids metabolism in placenta with early spontaneous pregnancy loss (ESPL) in Chinese women. Seventy women with ESPL and 29 healthy pregnant women who asked for legal induced abortion were included in the case and control groups, respectively. The gestational age of the subject foetuses in both the case and control groups ranged from 4 to 10 weeks. The total fatty acids composition in the decidual and villous tissues was detected by gas-liquid chromatography using a standard method...
January 25, 2018: Food & Function
Hai-Tao Gao, Run Xu, Wei-Xin Cao, Qian-Nan Di, Rui-Xian Li, Lingeng Lu, Qian Xu, Shu-Qin Yu
Human beings are inevitably exposed to ubiquitous phthalate esters (PEs), and simultaneously ingesting high quantities of food emulsifiers via daily diet. Glycerin monostearate (GMS) is a widely used food emulsifier. The purposes of this study were to investigate the combined effects between the mixture of six frequently used PEs (MIXPs) and GMS on male rat reproductive system, and further to explore the underlying mechanisms. Male rats were orally administered either sodium carboxymethyl cellulose as controls or MIXPs at three different low-doses with or without GMS (200mg/kg/d) by gavage...
January 20, 2018: Toxicology and Applied Pharmacology
Wenping Xie, Wenpeng Zhang, Juan Ren, Wentao Li, Lili Zhou, Yuan Cui, Huiming Chen, Wenlian Yu, Xiaomei Zhuang, Zhenqing Zhang, Guolin Shen, Haishan Li
Triclocarban (TCC) has been identified as a new environmental pollutant that is potentially hazardous to human health; however, the effects of short-term TCC exposure on cardiac function are not known. The aim of this study was to use metabonomics and molecular biology techniques to systematically elucidate the molecular mechanisms of the TCC-induced effects on cardiac function in mice. Our results show that TCC inhibited the uptake, synthesis and oxidation of fatty acids, suppressed the TCA cycle, and increased aerobic glycolysis levels in heart tissue after short-term TCC exposure...
January 24, 2018: Journal of Agricultural and Food Chemistry
Yuan Zhou, Lihui Men, Zifeng Pi, Mengying Wei, Fengrui Song, Chunfang Zhao, Zhiqiang Liu
Modern studies have indicated Gardenia jasminoides Ellis (G. jasminoides) showed positive effect in treating type 2 diabetes mellitus (T2DM). In this study, sixty streptozotocin induced T2DM rats were divided into four groups: type 2 diabetes control group, geniposide treated group, total iridoid glycosides treated group, and crude extraction of gardenlae fructus treated group, other ten healthy rats were the healthy control group. During 12 weeks treatment, rat's feces samples were collected for the metabolomics study based on mass spectrometry technique...
January 24, 2018: Journal of Agricultural and Food Chemistry
Xiaozheng Du, Bo Yuan, Jinhai Wang, Xinghua Zhang, Liang Tian, Tingzhuo Zhang, Xinglan Li, Fengfan Zhang
OBJECTIVE: To explore heat-reinforcing needling for the metabolite profiling changes in serum of rheumatoid arthritis (RA) rabbits with liquid chromatograph-mass spectrometer (LC-MS) technique, and to investigate its mechanisms. METHODS: Forty clean purple blue rabbits were randomized into a normal group, a model group, a reinforcing-reducing needling (RRN) group, a twirling-reinforcing needling (TRN) group, and a heat-reinforcing needling (HRN) group, 8 cases in each group...
September 12, 2017: Zhongguo Zhen Jiu, Chinese Acupuncture & Moxibustion
Hua-Hui Hu, Xiao-Long Huang, Ren-Fu Quan, Zong-Bao Yang, Jing-Jing Xu
OBJECTIVE: To establish the rat model of acute spinal cord injury, followed by aprimary study on this model with ¹H NMR based on metabonomics and to explore the metabonomics and biomarkers of spinal cord injury rat. METHODS: Twenty eight-week-old adult male SD rats of clean grade, with body weight of (200±10) g, were divided into sham operation group and model group in accordance with the law of random numbers, and every group had 10 rats. The rats of sham operation group were operated without damaging the spinal cord, and rats of model group were made an animal model of spinal cord incomplete injury according to the modified Allen's method...
February 25, 2017: Zhongguo Gu Shang, China Journal of Orthopaedics and Traumatology
Yazhou Zhao, Jianmei Zhang, Yanping Chen, Zhiguo Li, Hongyi Nie, Wenjun Peng, Songkun Su
To improve our understanding of the disturbed metabolic pathways and cellular responses triggered by honeybee venom stimulation, we compared the changes in serum metabolites rats, either stimulated or not by honeybee venom by performing 1H NMR spectrometry-based metabonomics to identify potential biomarkers. In this study, 65 metabolites were structurally confirmed and quantified and the following results were obtained. Firstly, by pattern recognition analysis, 14 metabolites were selected as potential biomarkers 3 hrs after venom stimulation...
January 11, 2018: Journal of Agricultural and Food Chemistry
Bingyu Yang, Chang Wang, Yiyu Xie, Liangjing Xu, Xiaojin Wu, Depei Wu
To investigate the potential biomarkers associated with Chronic Myeloid Leukemia (CML), reveal the metabolite changes related to the continuous phases of tyrosine kinase inhibitors (TKI) and find the potential biomarkers associated with treatment effects. 52 patients with CML and matched 26 healthy people were enrolled as discovery set. Another 194 randomly selected CML patients treated with TKI were chosen as external validation set. Plasma samples from the patients and controls were profiled by using gas chromatography-mass spectrometry (GC-MS) based metabonomic approach...
January 8, 2018: Cancer Science
Yang Lu, Hui Li, Yue Geng
δ-Tocopherol (δ-TOH) is a form of vitamin E with higher bioactivity. In this study, we studied the bioactivity of δ-TOH using the IC50 of δ-TOH on RAW264.7 (80 μM) and K562 (110 μM) cells. We compared the differential metabolites from the cell lines with and without δ-TOH treatment by 1H-NMR metabonomics analysis. It was found that δ-TOH affected the protein biosynthesis, betaine metabolism, and urea cycle in various ways in both cell lines. Metabolic levels of the cell lines were changed after treatment with δ-TOH as differential metabolites were produced...
January 9, 2018: Journal of Agricultural and Food Chemistry
Mengjie Yu, Tianxin Xiang, Xiaoping Wu, Shouhua Zhang, Wenlong Yang, Yu Zhang, Qiang Chen, Shuilin Sun, Baogang Xie
BACKGROUND: The discovery of new metabolic markers may be helpful for early diagnosis of acute pediatric appendicitis (APA). However, no studies have been reported regarding identification of potential metabolic markers for the APA diagnosis by metabonomics. METHODS: Serum samples of APA (n=32), non-appendicitis inflammation (NAI, n=32) and healthy children (HS, n=65) were analyzed by the 1H NMR-based metabonomics. A logistic regression model was established to screen the most efficient markers combinations for classification...
January 8, 2018: Clinical Chemistry and Laboratory Medicine: CCLM
Yan Zhang, Haitao Li, Tao Hu, Hui Li, Gang Jin, Yang Zhang
This study was performed to evaluate the hepatoprotective effect of FVP on carbon tetrachloride induced acute liver injury rats. Transaminase levels, antioxidant effect and histopathology were used to assess the recovery of FVP treated acute liver injury rats. Metabolomic analysis of liver homogenate based on GC-MS was used to obtain a global understanding of metabolic change between acute liver injury model rats and FVP-treated rats in order to assess the underlying mechanisms. Multivariate statistical approaches such as principal component analysis and orthogonal projection to latent structure square-discriminant analysis revealed distinctions among the normal, liver injury model, and FVP groups...
January 2, 2018: International Journal of Biological Macromolecules
Jingcheng Wang, Yuanlong Hou, Zhiying Jia, Xie Xie, Jiajian Liu, Yani Kang, Xin Wang, Xiaoyan Wang, Wei Jia
Different components of P. ginseng have different properties and medicinal effects. Metabonomics was a perspective approach to analyze global endogenous metabolites response to physiological and pathological processe. In this study, untargeted -metabonomics method using GC/TOFMS combined with multivariate statistical techniques was applied to compare entire metabolite differences and the anti-stress variations among four components of P. ginseng, total ginsenosides (TG), panaxadiol (PD), panaxatriol (PT) and ginseng polysaccharide (PS), in Sprague Dawley rats...
January 5, 2018: Journal of Proteome Research
Yanyan Xu, Yiwei Zhao, Jiabin Xie, Xue Sheng, Yubo Li, Yanjun Zhang
Psoraleae Fructus is the dry and mature fruit of leguminous plant Psoralea corylifolia L., with the activity of warming kidney and enhancing yang, warming spleen, and other effects. However, large doses can cause liver and kidney toxicity. Therefore, it is necessary to evaluate the toxicity of Psoraleae Fructus systematically. Although traditional biochemical indicators and pathological tests have been used to evaluate the safety of drug, these methods lack sensitivity and specificity, so a fast and sensitive analytical method is urgently needed...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
Yu Guo, Zhongfeng Li, Xinfeng Liu, Xiaolan Su, Yijie Li, Jiajie Zhu, Yilin Song, Ping Zhang, Jiande D Z Chen, Ruhan Wei, Jianqin Yang, Wei Wei
1H NMR-based metabolic profiling combined with multivariate data analysis was used to explore the metabolic phenotype of functional dyspepsia (FD) in stressed rats and evaluate the intervention effects of the Chinese medicine Weikangning (WKN). After a 7-day period of model establishment, a 14-day drug administration schedule was conducted in a WKN-treated group of rats, with the model and normal control groups serving as negative controls. Based on 1H NMR spectra of urine and serum from rats, PCA, PLS-DA, and OPLS-DA were performed to identify changing metabolic profiles...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
H Yan, X Y Zhuo, B H Shen, P Xiang, M Shen
OBJECTIVES: To analyse the metabolic changes in urine of rats with brodifacoum intoxication, and to reveal the molecular mechanism of brodifacoum-induced toxicity on rats. METHODS: By establishing a brodifacoum poisoning rats model, the urine metabolic profiling data of rats were acquired using high performance liquid chromatography-time of flight mass spectrometry (HPLC-TOF-MS). The orthogonal partial least squares analysis-discrimination analysis (OPLS-DA) was applied for the multivariate statistics and the discovery of differential metabolites closely related to toxicity of brodifacoum...
June 2017: Fa Yi Xue za Zhi
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