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https://www.readbyqxmd.com/read/28105254/disease-monitoring-of-hepatocellular-carcinoma-through-metabolomics
#1
REVIEW
Asem I Fitian, Roniel Cabrera
We elucidate major pathways of hepatocarcinogenesis and accurate diagnostic metabolomic biomarkers of hepatocellular carcinoma (HCC) identified by contemporary HCC metabolomics studies, and delineate a model HCC metabolomics study design. A literature search was carried out on Pubmed for HCC metabolomics articles published in English. All relevant articles were accessed in full text. Major search terms included "HCC", "metabolomics", "metabolomics", "metabonomic" and "biomarkers". We extracted clinical and demographic data on all patients and consolidated the lead candidate biomarkers, pathways, and diagnostic performance of metabolomic expression patterns reported by all studies in tables...
January 8, 2017: World Journal of Hepatology
https://www.readbyqxmd.com/read/28095303/gd-dtpa-induced-dynamic-metabonomic-changes-in-rat-biofluids
#2
Chuanling Wan, Youyang Zhan, Rong Xue, Yijie Wu, Xiaojing Li, Fengkui Pei
OBJECTIVES: The purposes of this study were (1) to detect the dynamic metabonomic changes induced by gadopentetate dimeglumine (Gd-DTPA) and (2) to investigate the potential metabolic disturbances associated with the pathogenesis of nephrogenic systemic fibrosis (NSF) at the early stage. METHODS: A nuclear magnetic resonance (NMR)-based metabolomics approach was used to investigate the urinary and serum metabolic changes induced by a single tail vein injection of Gd-DTPA (dosed at 2 and 5mmol/kg body weight) in rats...
January 14, 2017: Magnetic Resonance Imaging
https://www.readbyqxmd.com/read/28093038/metabonomic-strategy-to-the-evaluation-of-chinese-medicine-salvia-miltiorrhiza-and-dalbergia-odorifera-interfering-myocardial-ischemia-reperfusion-injury-in-rats
#3
Fei Mu, Jialin Duan, Haixu Bian, Xiaohu Zhai, Peijin Shang, Rui Lin, Meina Zhao, Dongmei Hu, Ying Yin, Aidong Wen, Miaomiao Xi
Extract of Salvia miltiorrhiza and Dalbergia Odorifera (SM-DOO) has been traditionally used for the prevention and treatment of cardiovascular diseases. However, information regarding the pharmacodyamic material basis and potential mechanism remain unknown. Male Sprague-Dawley rats were divided into four groups: Sham, Model, Diltiazem and SM-DOO group, n=6. Rats were pretreated with homologous drugs for 7 days, then subjected to 30 min of ischemia followed by 180 min of reperfusion. Cardioprotection effects of SM-DOO on myocardial ischemia/reperfusion (MI/R) injury rats were examined by hemodynamic, infarct area, histopathology, biochemical indicators and western blot analysis...
January 16, 2017: Rejuvenation Research
https://www.readbyqxmd.com/read/28092089/time-dependent-lactate-production-and-amino-acid-utilization-in-cultured-astrocytes-under-high-glucose-exposure
#4
Dan Wang, Liangcai Zhao, Hong Zheng, Minjian Dong, Linlin Pan, Xi Zhang, Huajie Zhang, Hongchang Gao
Accumulating investigations have focused on the severity of central nervous system (CNS) complications in diabetic patients. The effects of the high glucose (HG) probably attribute to the metabolic disturbances in CNS. Astrocytes, with powerful ability of metabolic regulation, play crucial roles in physiological and pathological processes in CNS. Hence, an in-depth analysis as to metabolic alterations of astrocytes exposure to HG would facilitate to explore the underlying pathogenesis. In this study, the (1)H NMR-based metabonomic approach was performed to characterize the metabolic variations of intracellular metabolites and corresponding culture media in a time-dependent manner...
January 16, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28063302/metabonomics-study-of-the-therapeutic-mechanism-of-fenugreek-galactomannan-on-diabetic-hyperglycemia-in-rats-by-ultra-performance-liquid-chromatography-coupled-with-quadrupole-time-of-flight-mass-spectrometry
#5
Wenyue Jiang, Lu Gao, Pengdong Li, Hong Kan, Jiale Qu, Lihui Men, Zhiqiang Liu, Zhongying Liu
Fenugreek is a traditional plant for the treatment of diabetes. Galactomannan, an active major component in fenugreek seeds, has shown hypoglycemic activity. The present study was performed to investigate the therapeutic mechanism underlying fenugreek galactomannan (F-GAL) in treating diabetes, using a metabonomics approach based on ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS). The F-GAL used for study was highly purified, and its yield, purity, and galactose/mannose ratio were characterized by capillary zone electrophoresis (CZE) and a modified phenol-sulfuric acid method...
December 29, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28045162/biochemical-effects-of-venlafaxine-on-astrocytes-as-revealed-by-1-h-nmr-based-metabolic-profiling
#6
Lu Sun, Liang Fang, Bin Lian, Jin-Jun Xia, Chan-Juan Zhou, Ling Wang, Qiang Mao, Xin-Fa Wang, Xue Gong, Zi-Hong Liang, Shun-Jie Bai, Li Liao, Yu Wu, Peng Xie
As a serotonin-norepinephrine reuptake inhibitor [SNRI], venlafaxine is one of the most commonly prescribed clinical antidepressants, with a broad range of antidepressant effects. Accumulating evidence shows that venlafaxine may target astrocytes to exert its antidepressant activity, although the underlying pharmacological mechanisms remained largely unknown. Here, we used a (1)H nuclear magnetic resonance (NMR)-based metabonomics method coupled with multivariate statistical analysis to characterize the metabolic profiling of astrocytes treated with venlafaxine to explore the potential mechanism of its antidepressant effect...
January 3, 2017: Molecular BioSystems
https://www.readbyqxmd.com/read/28045158/metabolomics-and-proteomics-technologies-to-explore-the-herbal-preparation-affecting-metabolic-disorders-using-high-resolution-mass-spectrometry
#7
Aihua Zhang, Xiaohang Zhou, Hongwei Zhao, Shiyu Zou, Chung Wah Ma, Qi Liu, Hui Sun, Liang Liu, Xijun Wang
An integrative metabolomics and proteomics approach can provide novel insights in the understanding of biological systems. We have integrated proteome and metabolome data sets for a holistic view of the molecular mechanisms in disease. Using quantitative iTRAQ-LC-MS/MS proteomics coupled with UPLC-Q-TOF-HDMS based metabolomics, we determined the protein and metabolite expression changes in the kidney-yang deficiency syndrome (KYDS) rat model and further investigated the intervention effects of the Jinkui Shenqi Pill (JSP)...
January 3, 2017: Molecular BioSystems
https://www.readbyqxmd.com/read/28028715/study-on-the-intervention-effects-of-pinggan-prescription-on-spontaneously-hypertensive-rats-based-on-metabonomic-combined-with-pharmacodynamic-methods
#8
Jun Xie, Hai-Qiang Jiang, Yun-Lun Li, Lei Nie, Hong-Lei Zhou, Wen-Qing Yang
OBJECTIVE: To investigate the effects of Pinggan Prescription (, PGP) on hypertension by the associated methods of metabonomic and pharmacodynamic. METHODS: A total of 32 male spontaneously hypertensive rats (SHRs) were randomly divided into two groups by using the random number table method: a treatment group (n=18) and a model group (n=14). The Wistar rats (n=14) were used as the normal group. Different prescription were used to intervene three groups: the treatment group in which PGP extract was administered orally at a dose of 18...
December 27, 2016: Chinese Journal of Integrative Medicine
https://www.readbyqxmd.com/read/28017698/metabonomics-of-ageing-towards-understanding-metabolism-of-a-long-and-healthy-life
#9
REVIEW
Francois-Pierre J Martin, Ivan Montoliu, Martin Kussmann
Systems biology approaches have been increasingly employed in clinical studies to enhance our understanding of the role of genetics, environmental factors and their interactions on nutritional, health and disease status. Amongst the new omics technologies, metabonomics has emerged as a robust platform to capture metabolic and nutritional requirements by enabling, in a minimally invasive fashion, the monitoring of a wide range of biochemical compounds. Their variations reflect comprehensively the various molecular regulatory processes, which are tightly controlled and under the influence of genetics, diet, gut microbiota and other environmental factors...
December 23, 2016: Mechanisms of Ageing and Development
https://www.readbyqxmd.com/read/27978472/study-the-therapeutic-mechanism-of-amomum-compactum-in-gentamicin-induced-acute-kidney-injury-rat-based-on-a-back-propagation-neural-network-algorithm
#10
Xiye Wang, Hongmei Chen, Chun Chang, Mingyang Jiang, Xiulan Wang, Liang Xu
Acute kidney injury (AKI) is a major global public health problems, as it causes high morbidity and serious injury to renal function. However, the etiology for AKI is not very clear. In this study, a serum metabolite profile analysis was performed to identify potential biomarkers for gentamicin-induced AKI and to investigate the mechanism of action of Amomum compactum (AC) used for treatment. A metabonomics approach by ultra-performance liquid chromatography together with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was applied to perform the analysis...
January 1, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/27930812/assessing-2d-electrophoretic-mobility-spectroscopy-2d-mosy-for-analytical-applications
#11
Yuan Fang, Pavel V Yushmanov, István Furó
Electrophoretic displacement of charged entities phase modulates the spectrum acquired in electrophoretic NMR experiments and this modulation can be presented via 2D FT transformation as 2D MOSY (MObility SpectroscopY) spectra. We compare in various mixed solutions the chemical selectivity provided by 2D MOSY spectra to that provided by 2D DOSY (Diffusion Ordered SpectroscopY) spectra and demonstrate, under the conditions explored, a superior performance of the former method. 2D MOSY compares also favourably to closely related LC-NMR methods...
December 8, 2016: Magnetic Resonance in Chemistry: MRC
https://www.readbyqxmd.com/read/27881338/-plasma-metabonomics-of-guifu-dihuang-wan-in-the-treatment-of-yang-deficiency
#12
Ya Xiao, Yuan Jing, Jie-Yu Chen, Fei Li, Jing-Ru Cheng, Jian-Lu Bi, Ren Luo, Xiao-Shan Zhao
OBJECTIVE: To assess the effect of Guifu Dihuang Wan (GFDHW) in the treatment of yang deficiency and explore the underlying molecular mechanism. METHODS: Sixty-two participants without diseases were randomized into control group (n=31) and experimental group (n=31) and were given lifestyle intervention additional GFDHW treatment for a month. NMR technology was used for metabonomics analysis. RESULTS: Intervention with GFDHW resulted in significantly decreased conversion scores of yang deficiency in the experimental group compared with the control group (P<0...
November 20, 2016: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/27859443/metabolic-characterization-of-the-early-stage-of-hepatic-fibrosis-in-rat-using-gc-tof-ms-and-multivariate-data-analyses
#13
Hui Jiang, Jun-Mei Song, Peng-Fei Gao, Xiu-Juan Qin, Shuang-Zhi Xu, Jia-Fu Zhang
The aim of this study was to explore the changes in the urine metabolic spectrum in rats with the early stage of liver fibrosis using gas chromatography-time of flight/mass spectrometry (GC-TOF/MS), try to search for potential biomarkers and elucidate the probably metabonomic pathogenesis. The early stage of liver fibrosis was established with a single subcutaneously injection carbon tetrachloride (CCl4 ) twice each week for 4 weeks continuously. At the end of the experiment, GC-TOF/MS technology with multivariate statistical approaches such as principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) was used to analyze the changes in the metabolic spectrum trajectory and identify potential biomarkers...
November 17, 2016: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/27856104/the-investigation-of-anti-inflammatory-activity-of-yi-guanjian-decoction-by-serum-metabonomics-approach
#14
Sufang Shui, Xiaorong Cai, Rongqing Huang, Bingkun Xiao, Jianyun Yang
Yi Guanjian (YGJ), one of the Chinese herbal medicines most commonly used in western countries, reported to possess significant anti-inflammatary effects that inhibit the process of inflammation. However, the mechanisms underlying its anti-inflammation effects remain largely unresolved. This study was aimed to investigate the anti-inflammatory activity of YGJ and to explore its potential anti-inflammatory mechanisms by serum metabonomics approach. An xylene-induced mouse right-ear-edema model was used as an inflammatory response in vivo model...
January 30, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/27845778/identification-of-sex-specific-urinary-biomarkers-for-major-depressive-disorder-by-combined-application-of-nmr-and-gc-ms-based-metabonomics
#15
P Zheng, J-J Chen, C-J Zhou, L Zeng, K-W Li, L Sun, M-L Liu, D Zhu, Z-H Liang, P Xie
Women are more vulnerable to major depressive disorder (MDD) than men. However, molecular biomarkers of sex differences are limited. Here we combined gas chromatography-mass spectrometry (GC-MS)- and nuclear magnetic resonance (NMR)-based metabonomics to investigate sex differences of urinary metabolite markers in MDD, and further explore their potential of diagnosing MDD. Consequently, the metabolite signatures of women and men MDD subjects were significantly different from of that in their respective healthy controls (HCs)...
November 15, 2016: Translational Psychiatry
https://www.readbyqxmd.com/read/27845029/establishment-and-optimization-of-nmr-based-cell-metabonomics-study-protocols-for-neonatal-sprague-dawley-rat-cardiomyocytes
#16
Ming Zhang, Bo Sun, Qi Zhang, Rong Gao, Qiao Liu, Fangting Dong, Haiqin Fang, Shuangqing Peng, Famei Li, Xianzhong Yan
A quenching, harvesting, and extraction protocol was optimized for cardiomyocytes NMR metabonomics analysis in this study. Trypsin treatment and direct scraping cells in acetonitrile were compared for sample harvesting. The results showed trypsin treatment cause normalized concentration increasing of phosphocholine and metabolites leakage, since the trypsin-induced membrane broken and long term harvesting procedures. Then the intracellular metabolite extraction efficiency of methanol and acetonitrile were compared...
January 15, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/27823631/pharmacokinetics-and-pharmacodynamics-study-of-rhein-treating-renal-fibrosis-based-on-metabonomics-approach
#17
Hao Sun, Guangwen Luo, Zheng Xiang, Xiaojun Cai, Dahui Chen
BACKGROUND: The selection of effect indicators in the pharmacokinetic/ pharmacodynamic study of complex diseases to describe the relationship between plasma concentration and effect indicators is difficult. PURPOSE: Three effect indicators of renal fibrosis were successfully determined. The relationship between pharmacokinetics and pharmacodynamics of rhein in rhubarb was elucidated. STUDY DESIGN: The study was a metabolomics analysis of rat plasma and pharmacokinetics/ pharmacodynamics of rhein...
December 1, 2016: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/27810280/-1-h-nmr-based-metabonomic-study-on-the-effects-of-epimedium-on-glucocorticoid-induced-osteoporosis
#18
Sina Pan, Ali Chen, Zhihui Han, Yaling Wang, Xin Lu, Yongxia Yang
Glucocorticoids are widely used in clinical practice for the treatment of many immune-mediated and inflammatory diseases, and glucocorticoid-induced osteoporosis (GIO) is the most common type of secondary osteoporosis. Epimedium is one of the most commonly used traditional Chinese medicines for treating osteoporosis. In the present study, we systematically analysed the metabonomic characteristics of GIO model rats and elucidated the therapeutic effect of Epimedium by using a (1)H NMR-based metabonomic approach in conjunction with multivariate data analysis...
December 1, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/27769393/simultaneous-profiling-of-eicosanoid-metabolome-in-plasma-by-uplc-ms-ms-method-application-to-identify-potential-makers-for-rheumatoid-arthritis
#19
Nannan Wang, Ronghua Dai, Weihui Wang, Yan Peng, Xiaoning Zhao, Kaishun Bi
To evaluate the potential relationship between rheumatoid arthritis and arachidonic acid (AA) metabonomics via cyclooxygenase (COX) and lipoxygenase (LOX) pathways, a UPLC-MS/MS method has been developed and validated for simultaneous and quantitative profiling of eicosanoid metabolome in rat plasma. The analytes were extracted from plasma samples by protein precipitation procedure, and then separated on a Shim-pack XR-ODS column with mobile phase A (0.05% formic acid in water, pH=3.3 adjusted with dilute ammonium hydroxide) and mobile phase B [methanol: acetonitrile (20:80, v/v)]...
December 1, 2016: Talanta
https://www.readbyqxmd.com/read/27746335/nmr-based-metabonomics-study-on-the-effect-of-gancao-in-the-attenuation-of-toxicity-in-rats-induced-by-fuzi
#20
Bo Sun, Xubin Wang, Ruili Cao, Qi Zhang, Qiao Liu, Meifeng Xu, Ming Zhang, Xiangbo Du, Fangting Dong, Xianzhong Yan
ETHNOPHARMACOLOGICAL RELEVANCE: Fuzi, the processed lateral root of Aconitum carmichaelii Debeaux, is a traditional Chinese medicine used for its analgesic, antipyretic, anti-rheumatoid arthritis and anti-inflammation effects; however, it is also well known for its toxicity. Gancao, the root of Glycyrrhiza uralensis Fisch., is often used concurrently with Fuzi to alleviate its toxicity. However, the mechanism of detoxication is still not well clear. AIM OF THE STUDY: In this study, the effect of Gancao on the metabolic changes induced by Fuzi was investigated by NMR-based metabonomic approaches...
December 4, 2016: Journal of Ethnopharmacology
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