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Bergljót Magnadóttir, Polly Hayes, Mariya Hristova, Birkir Þór Bragason, Anthony P Nicholas, Alister W Dodds, Sigríður Guðmundsdóttir, Sigrun Lange
Peptidylarginine deiminases (PADs) are calcium dependent enzymes with physiological and pathophysiological roles conserved throughout phylogeny. PADs promote post-translational deimination of protein arginine to citrulline, altering the structure and function of target proteins. Deiminated proteins were detected in the early developmental stages of cod from 11 days post fertilisation to 70 days post hatching. Deiminated proteins were present in mucosal surfaces and in liver, pancreas, spleen, gut, muscle, brain and eye during early cod larval development...
June 13, 2018: Developmental and Comparative Immunology
Helen Khan, Mariya Moosajee
No abstract text is available yet for this article.
June 15, 2018: Eye
Frank U Weiss, Mariya E Skube, Markus M Lerch
PURPOSE OF REVIEW: Genetic mutations in genes within and outside of the trypsin-dependent pathologic pathway have been found to be associated with chronic pancreatitis. This review highlights recent developments. RECENT FINDINGS: CTRB1-CTRB2 has been identified as a new risk locus for chronic pancreatitis and the disease mechanism may involve trypsin degradation. Misfolding mutations in PRSS1, CPA1, and CEL, as well as environmental stress factors like tobacco and alcohol can trigger endoplasmic reticulum stress (ER-Stress)...
June 8, 2018: Current Opinion in Gastroenterology
Mariya E Skube, Greg J Beilman
PURPOSE OF REVIEW: Patients suffering from chronic pancreatitis often require surgical intervention to treat their disease. This review discusses surgical options as well as reviews current trends and research in the operative management of chronic pancreatitis. RECENT FINDINGS: Relevant current topics in the field include the appropriate timing of surgery as well as the relative benefits of various procedures, particularly duodenum-preserving pancreatic head resection versus pancreaticoduodenectomy...
June 8, 2018: Current Opinion in Gastroenterology
Yasuyo Fukushi, Ayumi Nakamura, Chieko Itaki, Shinji Tokonami, Masatoshi Yamada, Yasushi Mariya
Following the accident at the Fukushima Daiichi Nuclear Power Plant in 2011, a number of evacuees were forced to live in temporary housing and suffered mental and physical stress. However, few reports have used objective or quantitative indicators to determine the evacuee's level of stress. The aim of the present study was to serially estimate the mental and physical stress of the evacuees from 2013 to 2015 by using the oxidative stress marker, urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG). A total of 773 evacuees from Namie town in Fukushima prefecture participated in the study...
July 2018: Experimental and Therapeutic Medicine
Sören Meyer, Mariya Markova, Gabriele Pohl, Talke A Marschall, Olga Pivovarova, Andreas F H Pfeiffer, Tanja Schwerdtle
Multi-element determination in human samples is very challenging. Especially in human intervention studies sample volumes are often limited to a few microliters and due to the high number of samples a high-throughput is indispensable. Here, we present a state-of-the-art ICP-MS/MS-based method for the analysis of essential (trace) elements, namely Mg, Ca, Fe, Cu, Zn, Mo, Se and I, as well as food-relevant toxic elements such as As and Cd. The developed method was validated regarding linearity of the calibration curves, method LODs and LOQs, selectivity and trueness as well as precision...
September 2018: Journal of Trace Elements in Medicine and Biology
Mariya Lazarova, Frank Lőhr, Ralf-Bernhardt Rues, Robin Kleebach, Volker Dötsch, Frank Bernhard
NMR studies of large proteins are complicated by pronounced spectral overlap and large line width. Re-ducing complexity by [13C, 1H] selective labelling of L-Val, L-Leu and/or L-Ile residues in combination with optional perdeuteration is therefore commonly approached by supplying labelled amino acid precursors into bacterial expression cultures, although often compromised by high label costs, precursor instability and label scrambling. Cell-free expres-sion combines efficient production of membrane proteins with significant advantages for protein labelling such as small reaction volumes, defined amino acid pools, and reliable label incorporation...
June 12, 2018: ACS Chemical Biology
Ekaterina V Pavlova, Galina S Alekseeva, Mariya N Erofeeva, Nina A Vasilieva, Andrey V Tchabovsky, Sergey V Naidenko
Blood analysis has recently become a popular tool to assess the welfare of the wild cats. However, the estimates of blood parameters may depend on the sampling method. We have tested (1) if the sampling procedure influences blood parameters and (2) what parameters are the most efficient in assessing the physiological status in wild cat species. We assessed the effect of handling time on red blood cells (RBC) and white blood cells (WBC) counts, the ratio of neutrophils to lymphocytes (N/L ratio), and serum cortisol level within 1 hr after the capture of the animal in six far-east wild cats (Prionailurus bengalensis euptilura)...
June 12, 2018: Journal of Experimental Zoology. Part A, Ecological and Integrative Physiology
Christian Njatcha, Mariya Farooqui, Adam Kornberg, Daniel E Johnson, Jennifer R Grandis, Jill M Siegfried
Constitutively activated STAT3 plays a critical role in non-small cell lung carcinoma (NSCLC) progression by mediating proliferation and survival. STAT 3 activation in normal cells is transient, making it an attractive target for NSCLC therapy. The therapeutic potential of blocking STAT3 in NSCLC was assessed utilizing a decoy approach by ligating a double-stranded 15-mer oligonucleotide that corresponds to the STAT3 response element of STAT3-target genes, to produce a cyclic STAT3 decoy (CS3D). The decoy was evaluated using NSCLC cells containing either wild-type (WT) EGFR (201T) or mutant EGFR with an additional EGFRi resistance mutation (H1975)...
June 11, 2018: Molecular Cancer Therapeutics
Irina Spacova, Mariya I Petrova, Astrid Fremau, Lore Pollaris, Jeroen Vanoirbeek, Jan L Ceuppens, Sven Seys, Sarah Lebeer
BACKGROUND: There is an increasing interest in targeted application of probiotic bacteria for prevention and treatment of airway diseases, including allergies. Here, we investigated the beneficial effects of preventive intranasal treatment with probiotics Lactobacillus rhamnosus GG and L. rhamnosus GR-1 in a mouse model of allergic asthma. METHODS: L. rhamnosus was administered intranasally eight times on days 1-4 and 8-11 at 5x108 CFU/dose, followed by a two-week asthma induction protocol with birch pollen extract on alternating days...
June 10, 2018: Allergy
Mariya P Dobreva, Vanesa Abon Escalona, Kirstie A Lawson, Marina N Sanchez, Ljuba C Ponomarev, Paulo N G Pereira, Agata Stryjewska, Nathan Criem, Danny Huylebroeck, Susana M Chuva de Sousa Lopes, Stein Aerts, An Zwijsen
Upon gastrulation, the mammalian conceptus transforms rapidly from a simple bilayer into a multi-layered embryo enveloped by its extraembryonic membranes. Impaired development of the amnion, the innermost membrane, causes major malformations. To clarify the origin of the mouse amnion, we used single cell-labelling and clonal analysis. We identified four clone types with distinct clonal growth patterns in amniotic ectoderm (AmEc). Two main types have progenitors in extreme proximal-anterior epiblast. Early descendants initiate and expand AmEc posteriorly, while descendants of cells remaining anteriorly later expand AmEc from its anterior side...
June 8, 2018: Development
Sergey Morozov, Vasily Isakov, Mariya Konovalova
AIM: To investigate the effect of dietary fiber on symptoms and esophageal function testing parameters in non-erosive gastroesophageal reflux disease (GERD) (NERD) patients. METHODS: Thirty-six NERD patients with low (< 20 g/d) dietary fiber intake were enrolled in the study. They were examined with the use of symptom questionnaire (GERD-Q), high-resolution esophageal manometry, 24-h esophageal pH-impedance examinations, and food frequency questionnaire before and after 10 d of usual diet supplemented by psyllium 5...
June 7, 2018: World Journal of Gastroenterology: WJG
Laura Thei, Eridan Rocha-Ferreira, Donald Peebles, Gennadij Raivich, Mariya Hristova
Hypoxia-ischemia (HI) is a major cause of neonatal brain injury resulting in cerebral palsy, epilepsy, cognitive impairment and other neurological disabilities. The role of Extracellular signal-Regulated Kinase (ERK) isoforms and their MEK-dependent phosphorylation in HI has previously been explored but remains unresolved at cellular level. This is pertinent given the growing awareness of the role of non-neuronal cells in neuroprotection. Using a modified Rice-Vannuccci model of HI in the neonatal mouse we observed time and cell-dependent ERK phosphorylation (pERK), with strongly up-regulated pERK immunoreactivity first in periventricular white matter axons within 15-45 min of HI, followed by forebrain astrocytes and neurons (1-4 h post HI), and return to baseline by 16 h...
June 6, 2018: Journal of Physiology
Emily Dauria, Marina Tolou-Shams, Halyna Skipalska, Mariya Bachmaha, Sara Hodgdon
Purpose Ukraine has one of the fastest growing HIV epidemics globally. Due to their engagement in high-risk behaviors, adolescents and emerging adults involved with the penitentiary system are at a particularly high risk of HIV-acquisition. To address the epidemic, young males (aged 14 to 20 years) in penitentiary institutions across Ukraine participated in a ten-week, group-based HIV-prevention intervention (STEPS). The paper aims to discuss these issues. Design/methodology/approach The authors analyzed clinical and programmatic services data collected as part of an evaluation of the STEPS intervention...
June 11, 2018: International Journal of Prisoner Health
Andrea N Niles, Mariya Smirnova, Joy Lin, Aoife O'Donovan
Depression and anxiety have been linked to elevated inflammation in cross-sectional and longitudinal studies. Yet, in terms of longitudinal studies, findings are inconsistent regarding whether depression predicts worsening inflammation or vice versa, and anxiety has been infrequently examined. Further, we know little about longitudinal relationships between inflammation and specific symptom profiles of depression and anxiety. The current study examined longitudinal associations between depression and anxiety symptoms and inflammation in 13,775 people (59% women, average age = 67) participating in the Health and Retirement Study - a population-based study focused on older adults...
May 28, 2018: Psychoneuroendocrinology
Marc Campo, Mariya P Shiyko, Mary Beth Kean, Lynne Roberts, Evangelos Pappas
BACKGROUND: Yoga is a popular complementary therapy for musculoskeletal pain. There are few studies however, that have examined the risks of recreational participation for causing musculoskeletal pain. OBJECTIVES: To examine the relationship between musculoskeletal pain and recreational yoga participation. METHODS: This was a prospective cohort study with one-year follow-up. Data were collected via electronic questionnaires, one year apart...
April 2018: Journal of Bodywork and Movement Therapies
Margot R F Reijnders, Kerry A Miller, Mohsan Alvi, Jacqueline A C Goos, Melissa M Lees, Anna de Burca, Alex Henderson, Alison Kraus, Barbara Mikat, Bert B A de Vries, Bertrand Isidor, Bronwyn Kerr, Carlo Marcelis, Caroline Schluth-Bolard, Charu Deshpande, Claudia A L Ruivenkamp, Dagmar Wieczorek, Diana Baralle, Edward M Blair, Hartmut Engels, Hermann-Josef Lüdecke, Jacqueline Eason, Gijs W E Santen, Jill Clayton-Smith, Kate Chandler, Katrina Tatton-Brown, Katelyn Payne, Katherine Helbig, Kelly Radtke, Kimberly M Nugent, Kirsten Cremer, Tim M Strom, Lynne M Bird, Margje Sinnema, Maria Bitner-Glindzicz, Marieke F van Dooren, Marielle Alders, Marije Koopmans, Lauren Brick, Mariya Kozenko, Megan L Harline, Merel Klaassens, Michelle Steinraths, Nicola S Cooper, Patrick Edery, Patrick Yap, Paulien A Terhal, Peter J van der Spek, Phillis Lakeman, Rachel L Taylor, Rebecca O Littlejohn, Rolph Pfundt, Saadet Mercimek-Andrews, Alexander P A Stegmann, Sarina G Kant, Scott McLean, Shelagh Joss, Sigrid M A Swagemakers, Sofia Douzgou, Steven A Wall, Sébastien Küry, Eduardo Calpena, Nils Koelling, Simon J McGowan, Stephen R F Twigg, Irene M J Mathijssen, Christoffer Nellaker, Han G Brunner, Andrew O M Wilkie
Next-generation sequencing is a powerful tool for the discovery of genes related to neurodevelopmental disorders (NDDs). Here, we report the identification of a distinct syndrome due to de novo or inherited heterozygous mutations in Tousled-like kinase 2 (TLK2) in 38 unrelated individuals and two affected mothers, using whole-exome and whole-genome sequencing technologies, matchmaker databases, and international collaborations. Affected individuals had a consistent phenotype, characterized by mild-borderline neurodevelopmental delay (86%), behavioral disorders (68%), severe gastro-intestinal problems (63%), and facial dysmorphism including blepharophimosis (82%), telecanthus (74%), prominent nasal bridge (68%), broad nasal tip (66%), thin vermilion of the upper lip (62%), and upslanting palpebral fissures (55%)...
May 22, 2018: American Journal of Human Genetics
Tatyana V Moskvina, Michail Yu Shchelkanov, Mariya A Begun
There have been few reports on the diversity and prevalence of parasitic fauna of the endangered Siberian tiger, which inhabits the territory of the Russian Far East. The present review attempts to summarize the information about the parasitic fauna of wild Siberian tigers, which includes 15 helminths and 3 protozoan species. The most prevalent parasitic species was found to be Toxocara cati, followed by Toxascaris leonina. Another commonly recorded Platyhelminth species is Paragonimus westermani, which causes a lethal infection of the lung parenchyma in Siberian tigers...
May 31, 2018: Integrative Zoology
Muhammad Ali, Shahid Y Khan, Shivakumar Vasanth, Mariya R Ahmed, Ruiqiang Chen, Chan Hyun Na, Jason J Thomson, Caihong Qiu, John D Gottsch, S Amer Riazuddin
Purpose: Corneal endothelial cells (CECs) are critical in maintaining clarity of the cornea. This study was initiated to develop peripheral blood mononuclear cell (PBMC)-originated, induced pluripotent stem cell (iPSC)-derived CECs. Methods: We isolated PBMCs and programmed the mononuclear cells to generate iPSCs, which were differentiated to CECs through the neural crest cells (NCCs). The morphology of differentiating iPSCs was examined at regular intervals by phase contrast microscopy...
May 1, 2018: Investigative Ophthalmology & Visual Science
Owen Rhys Vaughan, Carlo A Rossi, Yuval Ginsberg, Anthony White, Mariya Hristova, Neil J Sebire, John Martin, Ian C Zachary, Donald M Peebles, Anna L David
Uterine artery application of adenoviral vascular endothelial growth factor gene therapy (Ad.VEGF-A165 ) increases uterine blood flow and fetal growth in experimental animals with fetal growth restriction (FGR). Whether Ad.VEGF-A165 reduces lifelong cardiovascular disease risk imposed by FGR remains unknown. Here, pregnant guinea pigs fed 70% normal food intake to induce FGR received Ad.VEGF-A165 (1x1010 viral particles, n=15) or vehicle (n=10), delivered to the external surface of the uterine arteries, in mid-pregnancy...
May 30, 2018: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
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