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Stem cell breast cancer

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https://www.readbyqxmd.com/read/29330806/fluorescence-activated-cell-sorting-of-murine-mammary-cancer-stem-like-cell-subpopulations-with-hif-activity
#1
Danielle L Brooks, Tiffany N Seagroves
Fluorescence-activated cell sorting (FACS) is a common method to identify and to isolate subpopulations within a complex mixture of cells based on their light scatter and fluorescent staining profiles. FACS is widely used to enrich for normal tissue and tumor cells that have stem cell potential. Whereas FACS protocols using conventional breast cancer cell lines are relatively routine, additional technical challenges are encountered when sorting for cell populations from freshly digested solid tumors, particularly for use in downstream cancer stem cell (CSC) assays...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29330805/in-vitro-assays-of-breast-cancer-stem-cells
#2
Debangshu Samanta, Gregg L Semenza
Aldehyde dehydrogenase and mammosphere assays enable the cost-effective quantification and characterization of cancer stem cells (CSCs) from cancer cell lines as well as cancer tissue. Here we describe the quantification of CSCs in breast cancer cell lines using aldehyde dehydrogenase and mammosphere assays under hypoxic (1% O2) and non-hypoxic (20% O2) culture conditions. Using this method, a significant enrichment of CSCs compared to bulk populations is observed when breast cancer cells are exposed to 1% O2 for 72 h...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29330486/new-generation-of-drug-delivery-systems-based-on-ginsenoside-rh2-lysine-and-arginine-treated-highly-porous-graphene-for-improving-anticancer-activity
#3
Hadi Zare-Zardini, Asghar Taheri-Kafrani, Ahmad Amiri, Abdol-Khalegh Bordbar
In this study, Rh2-treated graphene oxide (GO-Rh2), lysine-treated highly porous graphene (Gr-Lys), arginine-treated Gr (Gr-Arg), Rh2-treated Gr-Lys (Gr-Lys-Rh2) and Rh2-treated Gr-Arg (Gr-Arg-Rh2) were synthesized. MTT assay was used for evaluation of cytotoxicity of samples on ovarian cancer (OVCAR3), breast cancer (MDA-MB), Human melanoma (A375) and human mesenchymal stem cells (MSCs) cell lines. The percentage of apoptotic cells was determined by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29329589/enhanced-metastatic-capacity-of-breast-cancer-cells-after-interaction-and-hybrid-formation-with-mesenchymal-stroma-stem-cells-msc
#4
Catharina Melzer, Juliane von der Ohe, Ralf Hass
BACKGROUND: Fusion of breast cancer cells with tumor-associated populations of the microenvironment including mesenchymal stroma/stem-like cells (MSC) represents a rare event in cell communication whereby the metastatic capacity of those hybrid cells remains unclear. METHODS: Functional changes were investigated in vitro and in vivo following spontaneous fusion and hybrid cell formation between primary human MSC and human MDA-MB-231 breast cancer cells. Thus, lentiviral eGFP-labeled MSC and breast cancer cells labeled with mcherry resulted in dual-fluorescing hybrid cells after co-culture...
January 5, 2018: Cell Communication and Signaling: CCS
https://www.readbyqxmd.com/read/29328395/bioinformatic-identification-of-chemoresistance-associated-micrornas-in-breast-cancer-based-on-microarray-data
#5
Ya-Wen Wang, Weiguo Zhang, Rong Ma
Breast cancer is the most commonly diagnosed cancer among females, and chemoresistance constitutes a major clinical obstacle to the treatment of this disease. MicroRNAs (miRNAs) are related to human cancer development, progression and drug resistance. To identify breast cancer chemoresistance-associated miRNAs, miRNA microarray dataset GSE71142, including five chemoresistant breast cancer tissues and five chemosensitive tissues, was downloaded from the Gene Expression Omnibus (GEO) database. Differentially expressed miRNAs (DE-miRNAs) were obtained by t-test and the potential target genes were predicted by miRWalk2...
January 10, 2018: Oncology Reports
https://www.readbyqxmd.com/read/29326438/conditional-knockout-of-n-myc-and-stat-interactor-disrupts-normal-mammary-development-and-enhances-metastatic-ability-of-mammary-tumors
#6
Hawley C Pruitt, Brandon J Metge, Shannon E Weeks, Dongquan Chen, Shi Wei, Robert A Kesterson, Lalita A Shevde, Rajeev S Samant
The process of organ development requires a delicate balance between cellular plasticity and differentiation. This balance is disrupted in cancer initiation and progression. N-Myc and STAT interactor (NMI: human or Nmi: murine) has emerged as a relevant player in the etiology of breast cancer. However, a fundamental understanding of its relevance to normal mammary biology is lacking. To gain insight into its normal function in mammary gland, we generated a mammary-specific Nmi knockout mouse model. We observed that Nmi protein expression is induced in mammary epithelium at the onset of pregnancy, in luminal cells and persists throughout lactation...
January 12, 2018: Oncogene
https://www.readbyqxmd.com/read/29323439/a-synthetic-binder-of-breast-cancer-stem-cells
#7
Luxi Chen, Chao Long, Kha Andy Minh Tran, Jiyong Lee
Cancer stem cells (CSCs) are associated with drug resistance, metastasis and recurrence of cancer. A synthetic binder of CSCs can provide a valuable tool to study the biology of CSCs and a lead to develop imaging, diagnostic and therapeutic agents targeting CSCs. Herein, a synthetic ligand (1) that specifically binds to CSCs over non-CSCs of breast cancer cells was identified for the first time via a cell-binding screening of a chemical library. The ligand 1 showed specific biding to CD24-/CD44+/ALDH+ CSC population of MCF-7 and MDA-MB-231...
January 11, 2018: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29321644/cox-2-induces-oncogenic-micro-rna-mir655-in-human-breast-cancer
#8
Mousumi Majumder, Leanna Dunn, Ling Liu, Asma Hasan, Krista Vincent, Muriel Brackstone, David Hess, Peeyush K Lala
We show that Cyclooxygenase-2 over-expression induces an oncogenic microRNA miR655 in human breast cancer cells by activation of EP4. MiR655 expression positively correlated with COX-2 in genetically disparate breast cancer cell lines and increased in all cell lines when grown as spheroids, implicating its link with stem-like cells (SLCs). Ectopic miR655 over-expression in MCF7 and SKBR3 cells resulted in increased proliferation, migration, invasion, spheroid formation and Epithelial to Masenchymal transition (EMT)...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29321622/evaluation-of-a-developmental-hierarchy-for-breast-cancer-cells-to-assess-risk-based-patient-selection-for-targeted-treatment
#9
Sarah A Bliss, Sunirmal Paul, Piotr W Pobiarzyn, Seda Ayer, Garima Sinha, Saumya Pant, Holly Hilton, Neha Sharma, Maria F Cunha, Daniel J Engelberth, Steven J Greco, Margarette Bryan, Magdalena J Kucia, Sham S Kakar, Mariusz Z Ratajczak, Pranela Rameshwar
This study proposes that a novel developmental hierarchy of breast cancer (BC) cells (BCCs) could predict treatment response and outcome. The continued challenge to treat BC requires stratification of BCCs into distinct subsets. This would provide insights on how BCCs evade treatment and adapt dormancy for decades. We selected three subsets, based on the relative expression of octamer-binding transcription factor 4 A (Oct4A) and then analysed each with Affymetrix gene chip. Oct4A is a stem cell gene and would separate subsets based on maturation...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29321615/functional-isolation-of-tumor-initiating-cells-using-microfluidic-based-migration-identifies-phosphatidylserine-decarboxylase-as-a-key-regulator
#10
Yu-Chih Chen, Brock Humphries, Riley Brien, Anne E Gibbons, Yu-Ting Chen, Tonela Qyli, Henry R Haley, Matthew E Pirone, Benjamin Chiang, Annie Xiao, Yu-Heng Cheng, Yi Luan, Zhixiong Zhang, Jason Cong, Kathryn E Luker, Gary D Luker, Euisik Yoon
Isolation of tumor-initiating cells currently relies on markers that do not reflect essential biologic functions of these cells. We proposed to overcome this limitation by isolating tumor-initiating cells based on enhanced migration, a function tightly linked to tumor-initiating potential through epithelial-to-mesenchymal transition (EMT). We developed a high-throughput microfluidic migration platform with automated cell tracking software and facile recovery of cells for downstream functional and genetic analyses...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29316250/dual-inhibition-of-wnt-and-yes-associated-protein-signaling-retards-the-growth-of-triple-negative-breast-cancer-in-both-mesenchymal-and-epithelial-states
#11
Andrew Sulaiman, Sarah McGarry, Li Li, Deyong Jia, Sarah Ooi, Christina Addison, Jim Dimitroulakos, Angel Arnaout, Carolyn Nessim, Zemin Yao, Guang Ji, Haiyan Song, Suresh Gadde, Xuguang Li, Lisheng Wang
Triple negative breast cancer (TNBC), the most refractory subtype of breast cancer to current treatments, accounts disproportionately for the majority of breast cancer related deaths. This is largely due to cancer plasticity and the development of cancer stem cells (CSCs). Recently, distinct yet interconvertible mesenchymal-like and epithelial-like states have been revealed in breast CSCs. Thus, strategies capable of simultaneously inhibiting bulk and CSC populations in both mesenchymal and epithelial states have yet to be developed...
January 9, 2018: Molecular Oncology
https://www.readbyqxmd.com/read/29311580/lsd1-activation-promotes-inducible-emt-programs-and-modulates-the-tumour-microenvironment-in-breast-cancer
#12
T Boulding, R D McCuaig, A Tan, K Hardy, F Wu, J Dunn, M Kalimutho, C R Sutton, J K Forwood, A G Bert, G J Goodall, L Malik, D Yip, J E Dahlstrom, A Zafar, K K Khanna, S Rao
Complex regulatory networks control epithelial-to-mesenchymal transition (EMT) but the underlying epigenetic control is poorly understood. Lysine-specific demethylase 1 (LSD1) is a key histone demethylase that alters the epigenetic landscape. Here we explored the role of LSD1 in global epigenetic regulation of EMT, cancer stem cells (CSCs), the tumour microenvironment, and therapeutic resistance in breast cancer. LSD1 induced pan-genomic gene expression in networks implicated in EMT and selectively elicits gene expression programs in CSCs whilst repressing non-CSC programs...
January 8, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29306324/stromal-cd10-expression-in-breast-cancer-correlates-with-tumor-invasion-and-cancer-stem-cell-phenotype
#13
Tahani Louhichi, Hanene Saad, Myriam Ben Dhiab, Sonia Ziadi, Mounir Trimeche
BACKGROUND: Previous investigations have indicated that CD10 is associated with biological aggressivity in human cancers, but the use of this marker for diagnosis and prognosis is more complex. The aim of this study was to evaluate the expression of CD10 in breast cancer and its association with the clinicopathological features. In addition, we investigated whether a relationship exists between CD10 expression and cancer stem cells. METHODS: CD10 expression was examined by the immunohistochemistry in a series of 133 invasive breast carcinoma cases...
January 6, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29305809/correction-to-tumour-suppressor-ep300-a-modulator-of-paclitaxel-resistance-and-stemness-is-downregulated-in-metaplastic-breast-cancer
#14
Muhammad Asaduzzaman, Stephanie Constantinou, Haoxiang Min, John Gallon, Meng-Lay Lin, Poonam Singh, Selina Raguz, Simak Ali, Sami Shousha, R Charles Coombes, Eric W-F Lam, Yunhui Hu, Ernesto Yagüe
In the original publication, Fig. 1 depicting the blot for EP300 in CAL51 cells (Fig. 1c) was unintentionally duplicated with that from MDA-MB-231 cells (Fig. 1d). The new figure given in this erratum depicts the correct EP300 blot in Fig. 1c.
January 5, 2018: Breast Cancer Research and Treatment
https://www.readbyqxmd.com/read/29305325/modeling-trastuzumab-related-cardiotoxicity-in-vitro-using-human-stem-cell-derived-cardiomyocytes
#15
Yosuke K Kurokawa, Michael R Shang, Rose T Yin, Steven C George
Trastuzumab (Herceptin®), a monoclonal antibody against the ErbB2 (HER2) receptor, has significantly improved clinical outcomes for HER2+ breast cancer patients. However, the drug also has known cardiotoxic side effects through mechanisms that are not fully understood. Here we utilized human induced pluripotent stem cell-derived cardiomyocytes (iPS-CMs) to model trastuzumab-related cardiotoxicity in vitro. We demonstrate that cardiotoxic effects of ErbB2 inhibition by trastuzumab can be recapitulated only when the cardioprotective effects of ErbB2/4 signaling is observed...
January 2, 2018: Toxicology Letters
https://www.readbyqxmd.com/read/29303076/utilizing-cancer-functional-gene-set-compound-networks-to-identify-putative-drugs-for-breast-cancer
#16
Tzu-Hung Hsiao, Yu-Chiao Chiu, Yu-Heng Chen, Yu-Ching Hsu, Hung-I Harry Chen, Eric Y Chuang, Yidong Chen
The number of anticancer drugs available currently is limited, and some of them have low treatment response rates. Moreover, developing a new drug for cancer therapy is labor intensive and sometimes cost prohibitive. Therefore, "repositioning" of known cancer treatment compounds can speed up the development time and potentially increase the response rate of cancer therapy. This study proposes a systems biology method for identifying new compound candidates for cancer treatment in two separate procedures. First, a "gene set-compound" network was constructed by conducting gene set enrichment analysis on the expression profile of responses to a compound...
January 5, 2018: Combinatorial Chemistry & High Throughput Screening
https://www.readbyqxmd.com/read/29302732/the-effects-of-platelet-rich-plasma-and-adipose-derived-stem-cells-on-neovascularization-and-fat-graft-survival
#17
Bing-Jun Xiong, Qiu-Wen Tan, Yu-Juan Chen, Yi Zhang, Di Zhang, Shen-Li Tang, Shu Zhang, Qing Lv
BACKGROUND: Adipose-derived stem cell (ADSCs)-assisted and platelet-rich plasma (PRP)-assisted lipofilling aim to enhance angiogenesis and cell proliferation and are promising techniques for lipofilling. This study aimed to compare the outcomes of ADSCs-assisted and PRP-assisted lipofilling. METHODS: Adipose tissue and human venous blood were obtained from women with early breast cancer. Human ADSCs were isolated and amplified in vitro. PRP was extracted through double centrifugation...
January 4, 2018: Aesthetic Plastic Surgery
https://www.readbyqxmd.com/read/29301945/modulation-of-glutathione-promotes-apoptosis-in-triple-negative-breast-cancer-cells
#18
Tara Miran, Andreas T J Vogg, Natascha Drude, Felix M Mottaghy, Agnieszka Morgenroth
Triple-negative breast cancer has an extremely high rate of relapse. This is particularly due to the existence and survival of cancer stem cells (CSCs) characterized by increased amounts of glutathione (GSH). In this study, we evaluated the potential of pharmacological GSH depletion to sensitize CSCs to ionizing radiotherapy with an I-125-labeled nucleoside analog, 5-iodo-4'-thio-2'-deoxyuridine (ITdU). CSCs were isolated using CD24-- and CD44+-specific microbeads. GSH and reactive oxygen species (ROS) were evaluated by fluorescence-activated cell sorting...
January 4, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29301415/ph-sensitive-polymeric-nanoparticles-of-mpeg-plga-pglu-with-hybrid-core-for-simultaneous-encapsulation-of-curcumin-and-doxorubicin-to-kill-the-heterogeneous-tumour-cells-in-breast-cancer
#19
Jian-Dong Yuan, De-Li ZhuGe, Meng-Qi Tong, Meng-Ting Lin, Xia-Fang Xu, Xing Tang, Ying-Zheng Zhao, He-Lin Xu
Most breast tumours are heterogeneous and not only contain the bulk of differentiated tumour cells but also a small population of highly tumorigenic and intrinsically drug-resistant cancer stem cells (CSCs). Herein, a pH-sensitive nanoparticle with simultaneous encapsulation of curcumin and doxorubicin (CURDOX-NPs) was prepared by using monomethoxy (polyethylene glycol)-b-P (D,L-lactic-co-glycolic acid)-b-P (L-glutamic acid) polymer to simultaneously target the differentiated tumor cells and CSCs. CURDOX-NPs had a mean diameter of 107...
January 4, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29300112/zoledronic-acid-overcomes-chemoresistance-by-sensitizing-cancer-stem-cells-to-apoptosis
#20
H Rouhrazi, N Turgan, G Oktem
Unlike low tumorigenic bulk tumor cells (non-CSCs), cancer stem cells (CSCs) are a subset of tumor cells that can self-renew and differentiate into different cancer subtypes. CSCs are considered responsible for tumor recurrence, distant metastasis, angiogenesis, and drug or radiation resistance. CSCs also are resistant to apoptosis. Zoledronic acid (ZA) is a third generation bisphosphonate that reduces cell proliferation and exhibits anti-tumor effects by inducing cell death in some malignancies; however, the effects of ZA on CSCs are unclear...
January 4, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
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