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https://www.readbyqxmd.com/read/29141579/a-missense-variant-in-the-coil1a-domain-of-the-keratin-25-gene-is-associated-with-the-dominant-curly-hair-coat-trait-crd-in-horse
#1
Caroline Morgenthaler, Mathieu Diribarne, Aurélien Capitan, Rachel Legendre, Romain Saintilan, Maïlys Gilles, Diane Esquerré, Rytis Juras, Anas Khanshour, Laurent Schibler, Gus Cothran
BACKGROUND: Curly horses present a variety of curl phenotypes that are associated with various degrees of curliness of coat, mane, tail and ear hairs. Their origin is still a matter of debate and several genetic hypotheses have been formulated to explain the diversity in phenotype, including the combination of autosomal dominant and recessive alleles. Our purpose was to map the autosomal dominant curly hair locus and identify the causal variant using genome-wide association study (GWAS) and whole-genome sequencing approaches...
November 15, 2017: Genetics, Selection, Evolution: GSE
https://www.readbyqxmd.com/read/29137821/equination-inoculation-of-horsepox-an-early-alternative-to-vaccination-inoculation-of-cowpox-and-the-potential-role-of-horsepox-virus-in-the-origin-of-the-smallpox-vaccine
#2
REVIEW
José Esparza, Livia Schrick, Clarissa R Damaso, Andreas Nitsche
For almost 150 years after Edward Jenner had published the "Inquiry" in 1798, it was generally assumed that the cowpox virus was the vaccine against smallpox. It was not until 1939 when it was shown that vaccinia, the smallpox vaccine virus, was serologically related but different from the cowpox virus. In the absence of a known natural host, vaccinia has been considered to be a laboratory virus that may have originated from mutational or recombinational events involving cowpox virus, variola viruses or some unknown ancestral Orthopoxvirus...
November 11, 2017: Vaccine
https://www.readbyqxmd.com/read/29134331/optimization-and-application-of-a-dna-launched-infectious-clone-of-equine-arteritis-virus
#3
Ting Qi, Xiaojun Wang
Reverse genetics is one of the most powerful tools in modern virology. Equine arteritis virus (EAV) is the prototype member of the Equartevirus. In this study, a new reverse genetics system for the recovery of equine arteritis virus from a cDNA plasmid, which contains viral cDNA sequence flanked by hammerhead ribozyme (HamRz) and hepatitis delta virus ribozyme (HdvRz) sequences in both terminals of the viral genome, was developed by optimization of the promoter and terminator regions. Cellular RNA polymerase II drove the transcription of the viral genome...
November 13, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29113823/genomic-comparison-of-bovine-papillomavirus-1-isolates-from-bovine-equine-and-asinine-lesional-tissue-samples
#4
C Koch, A S Ramsauer, M Drögemüller, M Ackermann, V Gerber, K Tobler
Several attempts have been made to categorize equid- and bovid-specific bovine papillomavirus 1 (BPV1) isolates based on sequence tags. This study includes newly determined sequence information from 33 BPV1 isolates of equine, asinine and bovine origin and investigates sequence bias due to host species. Twenty of the viral genomes were sequenced over their entire length and a further thirteen were sequenced, including flanking sequences, at two specific sites, the LCR and the E5 ORF. Alignment and analyses of the sequences did not reveal statistically significant site differences between the sequences of bovine and equid origin...
November 4, 2017: Virus Research
https://www.readbyqxmd.com/read/29103563/genetic-testing-as-a-tool-to-identify-horses-with-or-at-risk-for-ocular-disorders
#5
REVIEW
Rebecca R Bellone
Advances in equine genetics and genomics resources have enabled the understanding of some inherited ocular disorders and ocular manifestations. These ocular disorders include congenital stationary night blindness, equine recurrent uveitis, multiple congenital ocular anomalies, and squamous cell carcinoma. Genetic testing can identify horses with or at risk for disease and thus can assist in clinical management. In addition, genetic testing can identify horses that are carriers and thus can inform breeding decisions...
December 2017: Veterinary Clinics of North America. Equine Practice
https://www.readbyqxmd.com/read/29094242/molecular-characterization-of-a-genetically-divergent-equine-pegivirus-strain-identified-in-china
#6
Gang Lu, Cheng Fu, Ji Huang, Haibin Xu, Peixin Wu, Xiaokun Ping, Shujun Li
Equine pegivirus (EPgV) is a newly discovered equine virus, which is taxonomically classified in the Pegivirus genus of the Flaviviridae family. Until now, only the complete genome sequence of the first reported EPgV strain, from the USA (strain name: C0035) is available on online databases. Considering this, horse serum samples were collected from horses in China and screened for EPgV RNA by RT-PCR. One EPgV strain, LW/2016, was obtained and its near-complete genome sequence was acquired by standard PCR. Further analysis of its nucleotide sequence indicates LW/2016 is genetically divergent from C0035, with a nucleotide identity of 89...
November 2, 2017: Archives of Virology
https://www.readbyqxmd.com/read/29047258/identification-and-expression-analyses-of-equine-endogenous-retroviruses-in-horses
#7
Jeong-An Gim, Heui-Soo Kim
Endogenous retroviruses (ERVs) have been integrated into vertebrate genomes and have momentously affected host organisms. Horses (Equus caballus) have been domesticated and selected for elite racing ability over centuries. ERVs played an important role in the evolutionary diversification of the horse genome. In the present study, we identified six equine ERV families (EqERVs-E1, I1, M2, P1, S1, and Y4), their full-length viral open reading frames (ORFs), and elucidated their phylogenetic relationships. The divergence time of EqERV families assuming an evolutionary rate of 0...
October 2017: Molecules and Cells
https://www.readbyqxmd.com/read/29023800/to-pace-or-not-to-pace-a-pilot-study-of-four-and-five-gaited-icelandic-horses-homozygous-for-the-dmrt3-gait-keeper-mutation
#8
K Jäderkvist Fegraeus, I Hirschberg, T Árnason, L Andersson, B D Velie, L S Andersson, G Lindgren
The Icelandic horse is a breed known mainly for its ability to perform the ambling four-beat gait 'tölt' and the lateral two-beat gait pace. The natural ability of the breed to perform these alternative gaits is highly desired by breeders. Therefore, the discovery that a nonsense mutation (C>A) in the DMRT3 gene was the main genetic factor for horses' ability to perform gaits in addition to walk, trot and canter was of great interest. Although several studies have demonstrated that homozygosity for the DMRT3 mutation is important for the ability to pace, only about 70% of the homozygous mutant (AA) Icelandic horses are reported to pace...
October 11, 2017: Animal Genetics
https://www.readbyqxmd.com/read/29021424/detection-and-molecular-characterization-of-equine-infectious-anemia-virus-in-mongolian-horses
#9
Tumenjargal Sharav, Satoru Konnai, Nyamsuren Ochirkhuu, Erdene Ochir Ts, Hirohisa Mekata, Yoshihiro Sakoda, Takashi Umemura, Shiro Murata, Tungalag Chultemdorj, Kazuhiko Ohashi
The genetic characterization and actual prevalence of EIAV in Mongolian horse in the disease endemic region is currently unknown. Here, 11 of 776 horse serum samples from four Mongolian provinces tested positive on agar gel immunodiffusion test. Genomic DNA extracted from all seropositive samples was subjected to nested PCR assay. Among these, three samples tested positive with nested PCR assay and were identified by sequencing analysis based on long termination repeat and tat gene of the virus. Two of the three sequences were identical, with 94...
October 11, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29018124/straight-from-the-horse-s-mouth-genomic-epidemiology-of-an-icelandic-equine-epidemic
#10
Mark R Davies
Despite tight biosecurity measures, an outbreak of respiratory disease rapidly spread across the Icelandic equine population in 2010. Horse transportation was brought to a halt in order to contain the spread of the infectious agent. In a recent article, Björnsdóttir and colleagues (S. Björnsdóttir et al., mBio 8:e00826-17, 2017, https://doi.org/10.1128/mBio.00826-17) employ the power and resolution of "genomic epidemiology," the combination of whole genomic sequencing and epidemiological approaches, to examine the source and spread of the outbreak...
October 10, 2017: MBio
https://www.readbyqxmd.com/read/28993549/isolation-of-equine-papillomavirus-type-1-from-racing-horse-in-japan
#11
Jianbao Dong, Wei Zhu, Nanako Yamashita, James K Chambers, Kazuyuki Uchida, Atsutoshi Kuwano, Takeshi Haga
Although many studies have been conducted worldwide for Equus caballus papillomavirus (EcPV), limited information is available on the virus in Japan. We recently collected one classical viral papillomatosis sample (E150904) from a racing horse in Japan. Papillomavirus infection was confirmed by histopathology, immunohistochemistry and PCR assays, and the sample was diagnosed as epithelial papilloma. Full-length genome of the virus was cloned and sequenced. It was 7,613 bp in length and had the same genome organization with reported EcPV-1...
October 8, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/28946887/quadruplex-pcr-assay-for-identification-of-corynebacterium-pseudotuberculosis-differentiating-biovar-ovis-and-equi
#12
Sintia Almeida, Elaine M S Dorneles, Carlos Diniz, Vinícius Abreu, Cassiana Sousa, Jorianne Alves, Adriana Carneiro, Priscilla Bagano, Sharon Spier, Debmalya Barh, Andrey P Lage, Henrique Figueiredo, Vasco Azevedo
BACKGROUND: Corynebacterium pseudotuberculosis is classified into two biovars, nitrate-negative biovar Ovis which is the etiologic agent of caseous lymphadenitis in small ruminants and nitrate-positive biovar Equi, which causes abscesses and ulcerative lymphangitis in equines. The aim of this study was to develop a quadruplex PCR assay that would allow simultaneous detection and biovar-typing of C. pseudotuberculosis. METHODS: In the present study, genomes of C...
September 25, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28940727/whole-genome-sequencing-and-antigenic-analysis-of-the-first-equine-influenza-virus-identified-in-turkey
#13
Jacinta Gahan, Marie Garvey, Sarah Gildea, Gildea Gür, Anil Kagankaya, Ann Cullinane
BACKGROUND: In 2013 there was an outbreak of acute respiratory disease in racehorses in Turkey. The clinical signs were consistent with equine influenza (EI). OBJECTIVE: The aim was to confirm the cause of the outbreak and characterise the causal virus. METHODS: A pan-reactive influenza type A real time RT-PCR and a rapid antigen detection kit were used for confirmatory diagnosis of equine influenza virus (EIV). Immunological susceptibility to EIV was examined using Single Radial Haemolysis and ELISA...
September 21, 2017: Influenza and Other Respiratory Viruses
https://www.readbyqxmd.com/read/28929977/exposure-to-follicular-fluid-during-oocyte-maturation-and-oviductal-fluid-during-post-maturation-does-not-improve-in-vitro-embryo-production-in-the-horse
#14
Cécile Douet, Olivia Parodi, Nicola Antonio Martino, Giovanni Michele Lacalandra, Michele Nicassio, Fabrice Reigner, Stefan Deleuze, Maria Elena Dell'Aquila, Ghylène Goudet
Most wild equids and many domestic horse breeds are at risk of extinction, so there is an urgent need for genome resource banking. Embryos cryopreservation allows the preservation of genetics from male and female and is the fastest method to restore a breed. In the equine, embryo production in vitro would allow the production of several embryos per cycle. Intracytoplasmic sperm injection (ICSI) is used to generate horse embryos, but it requires expensive equipment and expertise in micromanipulation, and blastocyst development rates remain low...
September 20, 2017: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/28929497/genetic-diversity-and-paternal-origin-of-domestic-donkeys
#15
H Han, N Chen, J Jordana, C Li, T Sun, X Xia, X Zhao, C Ji, S Shen, J Yu, F Ainhoa, H Chen, C Lei, R Dang
Numerous studies have been conducted to investigate genetic diversity, origins and domestication of donkey using autosomal microsatellites and the mitochondrial genome, whereas the male-specific region of the Y chromosome of modern donkeys is largely uncharacterized. In the current study, 14 published equine Y chromosome-specific microsatellites (Y-STR) were investigated in 395 male donkey samples from China, Egypt, Spain and Peru using fluorescent labeled microsatellite markers. The results showed that seven Y-STRs-EcaYP9, EcaYM2, EcaYE2, EcaYE3, EcaYNO1, EcaYNO2 and EcaYNO4-were male specific and polymorphic, showing two to eight alleles in the donkeys studied...
September 19, 2017: Animal Genetics
https://www.readbyqxmd.com/read/28929022/development-and-evaluation-of-rapid-novel-isothermal-amplification-assays-for-important-veterinary-pathogens-chlamydia-psittaci-and-chlamydia-pecorum
#16
Martina Jelocnik, Md Mominul Islam, Danielle Madden, Cheryl Jenkins, James Branley, Scott Carver, Adam Polkinghorne
BACKGROUND: Chlamydia psittaci and Chlamydia pecorum are important veterinary pathogens, with the former also being responsible for zoonoses, and the latter adversely affecting koala populations in Australia and livestock globally. The rapid detection of these organisms is still challenging, particularly at the point-of-care (POC). In the present study, we developed and evaluated rapid, sensitive and robust C. psittaci-specific and C. pecorum-specific Loop Mediated Isothermal Amplification (LAMP) assays for detection of these pathogens...
2017: PeerJ
https://www.readbyqxmd.com/read/28888886/the-recombinant-ehv-1-vector-producing-cdv-hemagglutinin-as-potential-vaccine-against-canine-distemper
#17
Zihao Pan, Jin Liu, Jiale Ma, Qiuli Jin, Huochun Yao, Nikolaus Osterrieder
Canine distemper virus (CDV), is a pantropic agent of morbillivirus that causes fetal disease in dogs. Base on a broad host rang of CDV, the continued vaccines inoculation is unavoidable to pose gene recombination risk in vaccine virus and wild virus. The current study presents the construction of novel vectors, using equine herpesvirus type 1 (EHV-1) expressing the canine distemper virus (CDV). The recent field strain hemagglutinin protein and nucleoprotein were used for the construction of the viral vector vaccines...
October 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28884679/the-integration-of-a-macrophage-adapted-live-vaccine-strain-of-equine-infectious-anaemia-virus-eiav-in-the-horse-genome
#18
Qiang Liu, Xue-Feng Wang, Cheng Du, Yue-Zhi Lin, Jian Ma, Yu-Hong Wang, Jian-Hua Zhou, Xiaojun Wang
Integration is an important feature of retroviruses and retrovirus-based therapeutic transfection vectors. The non-primate lentivirus equine infectious anaemia virus (EIAV) primarily targets macrophages/monocytes in vivo. Investigation of the integration features of EIAVDLV121 strains, which are adapted to donkey monocyte-derived macrophages (MDMs), is of great interest. In this study, we analysed the integration features of EIAVDLV121 in equine MDMs during in vitro infection. Our previously published integration sites (IS) for EIAVFDDV13 in fetal equine dermal (FED) cells were also analysed in parallel as references...
September 7, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28846680/a-curated-catalog-of-canine-and-equine-keratin-genes
#19
Pierre Balmer, Anina Bauer, Shashikant Pujar, Kelly M McGarvey, Monika Welle, Arnaud Galichet, Eliane J Müller, Kim D Pruitt, Tosso Leeb, Vidhya Jagannathan
Keratins represent a large protein family with essential structural and functional roles in epithelial cells of skin, hair follicles, and other organs. During evolution the genes encoding keratins have undergone multiple rounds of duplication and humans have two clusters with a total of 55 functional keratin genes in their genomes. Due to the high similarity between different keratin paralogs and species-specific differences in gene content, the currently available keratin gene annotation in species with draft genome assemblies such as dog and horse is still imperfect...
2017: PloS One
https://www.readbyqxmd.com/read/28807043/an-equine-herpesvirus-type-1-ehv-1-vector-expressing-rift-valley-fever-virus-rvfv-gn-and-gc-induces-neutralizing-antibodies-in-sheep
#20
Abdelrahman Said, Mona Elmanzalawy, Guanggang Ma, Armando Mario Damiani, Nikolaus Osterrieder
Rift Valley fever virus (RVFV) is an arthropod-borne bunyavirus that can cause serious and fatal disease in humans and animals. RVFV is a negative-sense RNA virus of the Phlebovirus genus in the Bunyaviridae family. The main envelope RVFV glycoproteins, Gn and Gc, are encoded on the M segment of RVFV and known inducers of protective immunity. In an attempt to develop a safe and efficacious RVF vaccine, we constructed and tested a vectored equine herpesvirus type 1 (EHV-1) vaccine that expresses RVFV Gn and Gc...
August 14, 2017: Virology Journal
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